Beckman Coulter CytoFLEX User manual

B49006AS
December 2021
Instructions for Use
CytoFLEX Platform
CytoFLEX, CytoFLEX S, and CytoFLEX LX Flow Cytometers
For Research Use Only. Not for use in diagnostic procedures.
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

CytoFLEX Platform
Flow Cytometer
PN B49006AS (December 2021)
© 2021 Beckman Coulter, Inc.
All rights reserved.
Beckman Coulter, the stylized logo, and the Beckman
Coulter product and service marks mentioned herein are
trademarks or registered trademarks of Beckman Coulter,
Inc. in the United States and other countries.
Windows 7, Windows 8, and Windows 10 are registered
trademarks or trademarks of Microsoft Corporation in the
United States and/or other countries.
All other trademarks are the property of their respective
owners.
Contact Us
If you have any questions, contact our Customer Support
Center.
• Worldwide, find us via our website at
www.beckman.com/support/technical
• In the USA and Canada, call us at 1-800-369-0333.
• In Austria, call us at 0810 300484.
• In Germany, call us at 02151 333999.
• In Sweden, call us at [phone].
• In Netherlands, call us at [phone].
• In France, call us at 0825838306 6.
• In the UK, call us at [phone].
• In Ireland, call us at [phone].
• In Italy, call us at [phone].
• In other locales, contact your local Beckman Coulter
Representative.
Beckman Coulter (UK) Ltd.
Oakley Court
Kingsmead Business Park, London Road
High Wycombe
United Kingdom HP11 1JU
01494 441181
Glossary of Symbols is available at beckman.com/
techdocs (PN C24689).
May be covered by one or more pat. - see
www.beckman.com/patents
Original Instructions

B49006AS iii
Revision History
Initial Issue AA, 09/2014
Software Version 1.0
Issue AB, 12/2014
Software Version 1.0
Updates were made to the following sections: Symbol Explanations, Figure 1.10, Figure 1.11, Figure 9.3,
Figure 9.5, Figure 9.6, Figure 9.7, Disposal Of Electrical Instrumentation, Lifting and Carrying Instructions,
Replacing the Sheath Fluid Harness and/or Waste Harness, and Installing the Instrument and Connecting the
Equipment.
Issue AC, 02/2015
Software Version 1.1
Updates were made to the following sections:
Safety Notices
Symbol Explanations
CHAPTER 1, System Overview
Optical Fiber
Fluidics Module
Plate Loader Components
System Configuration
Instrument Specifications
Performance Characteristics
CHAPTER 2, Using the CytExpert Software
Start Page
Acquisition Screen
Test Tubes
Plot area
Status Bar
Analysis Screen
Compensation Experiment Screen
QC Experiment Screen
Software Menu
Graphic and Gating Styles
Software Settings
CHAPTER 3, Daily Startup
Opening the Software
Selecting the Plate Loader Sample Injection Mode [With Plate Loader]
Running the System Startup Program
Running the System Startup Program [With Plate Loader]
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

B49006AS
iv
Revision History
CHAPTER 4, Instrument Quality Control
Preparing the QC Sample [With Plate Loader]
Collecting QC Data
Confirming Results
Collecting QC Data [With Plate Loader]
Creating Levey-Jennings Charts
QC Result Manager
CHAPTER 5, Data Acquisition and Sample Analysis
Creating an Experiment
Creating an Experiment [With Plate Loader]
Sampling and Collecting Data
Setting the Channel and Label
Adjusting the Threshold
Creating Plots and Gates
Setting Customized Parameters
Setting Custom Statistics
Analyzing and Exporting Data
Exporting FCS Files
Exporting Plots or the Statistics Table of Multiple Tubes as Picture Files
Printing Graphics
CHAPTER 6, Compensation
Creating a Compensation Experiment
Creating a Compensation Experiment [With Plate Loader]
Manually Adjusting Compensation
Managing the Compensation Library
CHAPTER 7, Data Review
Calculating Sample Injection Volume and Concentration
Importing Previously Acquired Data
CHAPTER 9, Troubleshooting
Plate Loader Hazard Labels and Location
Table 9.2, Troubleshooting [With Plate Loader]
CHAPTER 10, Cleaning Procedures
Daily Clean [With Plate Loader]
Preparing the Instrument for Transport or Storage
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

B49006AS v
Revision History
CHAPTER 11, Replacement/Adjustment Procedures
Adding the Deep Clean Solution
Replacing the Sample Probe Assembly [With Plate Loader]
Changing the Sample Probe from the Single Tube Sample Station to the Plate Loader [With Plate Loader]
Changing the Sample Probe from the Plate Loader to the Single Tube Sample Station [With Plate Loader]
Replacing the Plate Holder [With Plate Loader]
Plate Loader Module Removal and Reinstallation [With Plate Loader]
Calibrating the Sample Flow Rate
Calibrating the Sample Flow Rate [With Plate Loader]
Changing Sample Mixing and Backflush Settings
Calibrating the Plate Position [With Plate Loader]
APPENDIX A, Instrument Installation
Power Source
Unpacking the Instrument and Inspecting the Materials for Defects or Omissions
Installing the CytExpert Software
Issue AD, 04/2015
Software Version 1.1
Updates were made to the following sections:
CHAPTER 1, System Overview
Cytometer
CHAPTER 2, Using the CytExpert Software
Analysis Screen
CHAPTER 8, Daily Shutdown
Shutting Down the Instrument
CHAPTER 11, Replacement/Adjustment Procedures
Changing the Event Rate Setting
Calibrating the Plate Position [With Plate Loader]
Issue AE, 11/2015
Software Version 1.1
Updates were made to the following sections:
CHAPTER 1, System Overview
Plate Loader Components
Plate Holder Components
Dimensions [CytoFLEX]
Acoustic Noise Level
Performance Characteristics
CHAPTER 4, Instrument Quality Control
Confirming Results
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

B49006AS
vi
Revision History
CHAPTER 9, Troubleshooting
Plate Loader Hazard Labels and Location
CHAPTER 10, Cleaning Procedures
Daily Clean
Daily Clean [With Plate Loader]
Preparing the Instrument for Transport or Storage
CHAPTER 11, Replacement/Adjustment Procedures
Replacing the Sample Probe Assembly [With Plate Loader]
Replacing the Plate Holder [With Plate Loader]
Plate Loader Module Removal and Reinstallation [With Plate Loader]
APPENDIX A, Instrument Installation
Installing the Instrument and Connecting the Equipment
Issue AF, 10/2016
Software Version 1.1
Updates were made to the following sections:
Safety Notices
Safety Precautions
Introduction
About this Manual
CHAPTER 11, Replacement/Adjustment Procedures
Changing the Sample Probe from the Single Tube Sample Station to the Plate Loader [With Plate Loader]
Changing the Sample Probe from the Plate Loader to the Single Tube Sample Station [With Plate Loader]
CytoFLEX Plate Loader Upgrade Kit
CytoFLEX Plate Loader Upgrade Kit Components
Changing the Sample Probe from the Single Tube Sample Station to the Plate Loader [With CytoFLEX Plate
Loader Upgrade Kit]
Changing the Sample Probe from the Plate Loader to the Single Tube Sample Station [With CytoFLEX Plate
Loader Upgrade Kit]
Issue AG, 1/2017
Software Version 2.0
Complete Revision
Issue AH, 3/2017
Software Version 2.0
Updates were made to the following sections:
Introduction
About this Manual
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

B49006AS vii
Revision History
CHAPTER 1, System Overview
Performance Characteristics [CytoFLEX]
Performance Characteristics [CytoFLEX LX]
CHAPTER 3, Daily Startup
Selecting the Plate Loader Sample Injection Mode [With Plate Loader]
CHAPTER 11, Replacement/Adjustment Procedures
Managing the Maintenance Reminder
Replacing the Sample Probe and/or the Sample Peristaltic Pump Tubing
Replacing the Sample Probe Assembly [With Plate Loader]
Changing the Sample Probe from the Single Tube Sample Station to the Plate Loader [CytoFLEX With Plate
Loader]
Changing the Sample Probe from the Plate Loader to the Single Tube Sample Station [CytoFLEX With Plate
Loader]
Changing Sample Mixing and Backflush Settings
APPENDIX A, Instrument Installation
Installing the Software [CytoFLEX]
Upgrading the CytExpert Software
Reinstalling the CytExpert Software
Added the following Chapter:
APPENDIX C, Sample Injection Mode Control Kit
Issue AJ, 5/2017
Software Version 2.0
Updates were made to the following sections:
CHAPTER 1, System Overview
Consumables and Supplies
CHAPTER 4, Instrument Quality Control and Standardization
Preparing the QC Sample
Preparing the QC Sample [With Plate Loader]
Importing Lot-Specific Target Values
CHAPTER 5, Data Acquisition and Sample Analysis
Setting Sample Wells
CHAPTER 9, Troubleshooting
Table 9.1
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

B49006AS
viii
Revision History
Issue AK, 8/2017
Software Version 2.1
Updates were made to the following sections:
CHAPTER 1, System Overview
Product Description
Wavelength Division Multiplexer (WDM)
WDM Optical Filter Mount Color Codes [CytoFLEX LX]
Optical Fiber
System Configuration
Performance Characteristics
CHAPTER 2, Using the CytExpert Software
Start Page
Acquisition Screen
Software Menu
Acquisition and Analysis Screen Menu
Advanced Menu
Account Menu
Log Menu
Signature Menu
Backup/Restore Menu
Viewing and Exporting User Logs
CHAPTER 3, Daily Startup
Logging into the Software
Selecting the Proper Sample Injection Mode
CHAPTER 4, Instrument Quality Control and Standardization
Overview
Importing Lot-Specific Target Values
Confirming Results
CHAPTER 5, Data Acquisition and Sample Analysis
Creating an Experiment [With Plate Loader]
Add Plate from Layout
Setting Sample Wells
Creating a Heat Map
Modifying Existing Heat Map Settings
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

B49006AS ix
Revision History
Creating Plots and Gates
Sampling and Collecting Data
Setting the Channel and Label
Setting Collection Conditions
Laser Settings
Analyzing and Exporting Data
Printing Graphics
CHAPTER 6, Compensation
Creating a Compensation Experiment
Manually Adjusting Compensation
CHAPTER 8, Daily Shutdown
Shutting Down the Instrument
CHAPTER 9, Troubleshooting
Laser Beam Hazards
Laser Warning Labels
Backup and Restore
CHAPTER 11, Replacement/Adjustment Procedures
Calibrating the Sample Flow Rate
APPENDIX A, Instrument Installation
Unpacking the Instrument and Inspecting the Materials for Defects or Omissions [CytoFLEX]
APPENDIX B, CytExpert Electronic Record Management
Figure B.3
Printing an Experiment Signature
APPENDIX D, Table of Hazardous Substances
APPENDIX D, Table of Hazardous Substances Name and Concentration [CytoFLEX 355]
Issue AL, 11/2017
Software Version 2.2
Updates were made to the following sections:
Safety Notices
Symbol Explanations
Introduction
Conventions Used
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

B49006AS
x
Revision History
CHAPTER 1, System Overview
Product Description
Sample Station
Plate Loader Components
Instrument Specifications
Performance Characteristics
CHAPTER 2, Using the CytExpert Software
Start Page
Software Menu
Advanced Menu
Plate Type Library
CHAPTER 3, Daily Startup
Selecting the Plate Loader Sample Injection Mode [With Plate Loader]
Running the System Startup Program [with the Single Tube Loader]
Running the System Startup Program [With Plate Loader]
CHAPTER 4, Instrument Quality Control and Standardization
Preparing the QC Sample [With Plate Loader]
Collecting QC Data [With Plate Loader]
Confirming Results
CHAPTER 9, Troubleshooting
Plate Loader Hazard Labels and Location
Troubleshooting Table
CHAPTER 10, Cleaning Procedures
Surface Cleaning and Disinfection
CHAPTER 11, Replacement/Adjustment Procedures
Managing the Maintenance Reminder
Replacing the Sheath Fluid Filter
Replacing the Plate Holder [With Plate Loader]
Calibrating the Plate Position [With Plate Loader]
APPENDIX A, Instrument Installation
Power Source
APPENDIX C, Sample Injection Mode Control Kit
Performance Characteristics [With the Sample Injection Mode Control Knob]
APPENDIX D, Deep Well Plate
Specimen Collection Plate Specifications
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

B49006AS xi
Revision History
Issue AM, 07/2018
Software Version 2.3
Updates were made to the following sections:
Safety Notices
Safety Precautions
Symbol Explanations
CHAPTER 1, System Overview
Wavelength Division Multiplexer (WDM)
Fluidics Module
Consumables and Supplies
Instrument Specifications
Performance Characteristics
CHAPTER 2, Using the CytExpert Software
Start Page
Software Menu
Settings Menu
Signature Menu
Role Management
Account Policies
User Management Operation Log
Plate Type Library
CHAPTER 3, Daily Startup
Logging into the Software
Logging Out of the Software
Locking the Account
Running the System Startup Program [with the Single Tube Loader]
Running the System Startup Program [With Plate Loader]
CHAPTER 4, Instrument Quality Control and Standardization
Overview
Importing Lot-Specific Target Values
Confirming Results
CHAPTER 5, Data Acquisition and Sample Analysis
Verifying, Selecting, Editing, and Creating Detector Configuration
Creating Plots and Gates
Printing Graphics
CHAPTER 10, Cleaning Procedures
Preparing the Instrument for Transport or Storage
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

B49006AS
xii
Revision History
CHAPTER 11, Replacement/Adjustment Procedures
Replacing the Sample Probe and/or the Sample Peristaltic Pump Tubing
Calibrating the Plate Position [With Plate Loader]
CHAPTER A, Instrument Installation
Installing the Instrument and Connecting the Equipment [CytoFLEX]
Installing the CytExpert Software
APPENDIX B, CytExpert Electronic Record Management
Experiment Management
Electronic Signature
User Management
APPENDIX E, Custom Optical Filters
Overview
Installing a Custom Optical Filter
Issue AN, 07/2019
Software Version 2.3
Updates were made to the following sections:
CHAPTER 1, System Overview
Main Components
Plate Holder Components
Sample Station
System Configuration
Instrument Specifications
CHAPTER 5, Data Acquisition and Sample Analysis
Creating Plots and Gates
Creating and Adjusting Auto Gates
Analyzing and Exporting Data
CHAPTER 6, Compensation
Creating a Compensation Experiment
Creating a Compensation Experiment [With Plate Loader]
CHAPTER 9, Troubleshooting
Biohazard Label and Location
CHAPTER 10, Cleaning Procedures
Daily Clean
Daily Clean [With Plate Loader]
Cleaning the Sample Station
CHAPTER 11, Replacement/Adjustment Procedures
Replacing the Sample Probe and/or the Sample Peristaltic Pump Tubing
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

B49006AS xiii
Revision History
CHAPTER A, Instrument Installation
Instrument Transportation and Storage
Installing the Software [CytoFLEX Platform]
APPENDIX E, Custom Optical Filters
Installing a Custom Optical Filter
Issue AP, 11/2019
Software Version 2.4
Updates were made to the following sections:
CHAPTER 1, System Overview
Wavelength Division Multiplexer (WDM)
Plate Holder Components
Cytometer
Reagent Limitations
CHAPTER 2, Using the CytExpert Software
Start Page
Software Menu
CHAPTER 3, Operation Principles
Overview
Sample Flow
Laser Beam Shaping
Cell Illumination
Light Collection, Separation and Measurement
Signal Processing
Data Storage
Automated Software Features
Parameters
Plot Display
Statistics
CHAPTER 4, Daily Startup
Logging into the Software
CHAPTER 5, Instrument Quality Control and Standardization
Overview
Preparing the QC Sample
Preparing the QC Sample [With Plate Loader]
Importing Lot-Specific Target Values
Collecting QC Data
Collecting QC Data [With Plate Loader]
Confirming Results
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

B49006AS
xiv
Revision History
Preparing the Standardization Sample
Generating Target Median Values
Adding a New Standardization Item
Performing the Standardization
Applying the Standardized Acquisition Settings
Standardization Target Library
CHAPTER 6, Data Acquisition and Sample Analysis
Creating an Experiment
Adjusting the Threshold
Verifying, Selecting, Editing, and Creating Detector Configuration
Sampling and Collecting Data [Without Plate Loader]
Sampling and Collecting Data [With Plate Loader]
Exporting Plots or the Statistics Table of Multiple Tubes as Picture Files
CHAPTER 7, Compensation
Creating a Compensation Experiment
CHAPTER 12, Replacement/Adjustment Procedures
Replacing the Plate Holder [With Plate Loader]
CHAPTER A, Instrument Installation
Installing the Instrument and Connecting the Equipment [CytoFLEX]
APPENDIX F, WDM Beam Splitter
Overview
Modifying Detector Configuration [With WDM Beam Splitter]
APPENDIX G, Cyber Security
Good Practices for Cyber Security
Issue AR 08/2021
Software Version 2.4
Updates were made to the following sections:
CHAPTER 1, System Overview
Consumables and Supplies
Performance Characteristics
CHAPTER 5, Instrument Quality Control and Standardization
Overview
Preparing the QC Sample
Preparing the QC Sample [With Plate Loader]
Importing Lot-Specific Target Values
Collecting QC Data
Collecting QC Data [With Plate Loader]
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

B49006AS xv
Revision History
CHAPTER 10, Troubleshooting
Hazard Labels and Locations
Troubleshooting Table
CHAPTER 12, Replacement/Adjustment Procedures
Changing Sample Mixing and Backflush Settings
APPENDIX C, Sample Injection Mode Control Kit
Performance Characteristics [With the Sample Injection Mode Control Knob]
Issue AS 12/2021
Software Version 2.5
Updates were made to the following sections:
CHAPTER 1, System Overview
Cytometer
CHAPTER 2, Using the CytExpert Software
Start Page
Software Menu
CHAPTER 4, Daily Startup
Logging into the Software
CHAPTER 5, Instrument Quality Control and Standardization
Collecting QC Data
Confirming Results
Applying the Standardized Acquisition Settings
CHAPTER 6, Data Acquisition and Sample Analysis
Creating Plots and Gates
Verifying, Selecting, Editing, and Creating Detector Configuration
Importing and Exporting Instrument Settings
APPENDIX A, Instrument Installation
Installing the CytExpert Software
APPENDIX G, Good Practices for Cyber Security
Drive Encryption
Protection from Malware Software
Operating System Updates
System Hardening
Note: Changes that are part of the most recent revisions are indicated in text by a bar in the margin of the
amended page.
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

B49006AS
xvi
Revision History

B49006AS xvii
Safety Notices
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate the instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact us.
Beckman Coulter, Inc. urges its customers to comply with all national health and safety standards
such as the use of barrier protection. This may include, but it is not limited to, protective eyewear,
gloves, and suitable laboratory attire when operating or maintaining this or any other automated
laboratory analyzer.
This manual assumes that users have basic knowledge of the Windows operating system, as well as
experience working with laboratory testing technology. Users are invited to consult the
appropriate documentation for such information.
Alerts for Danger, Warning, and Caution
DANGER
DANGER indicates an imminently hazardous situation which, if not avoided, will
result in death or serious injury.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, could
result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe
practices.

B49006AS
xviii
Safety Notices
Safety Precautions
Safety Precautions
WARNING
Risk of operator injury if:
• All doors, covers and panels are not closed and secured in place prior to and
during instrument operation.
• The integrity of safety interlocks and sensors is compromised.
• Instrument alarms and error messages are not acknowledged and acted upon.
•Youcontactmovingparts.
• You mishandle broken parts.
• Doors, covers and panels are not opened, closed, removed and/or replaced
with care.
• Improper tools are used for troubleshooting.
To avoid injury:
• Keep doors, covers and panels closed and secured in place while the
instrument is in use.
• Take full advantage of the safety features of the instrument.
• Acknowledge and act upon instrument alarms and error messages.
• Keep away from moving parts.
• Report any broken parts to your Beckman Coulter Representative.
• Open/remove and close/replace doors, covers and panels with care.
• Use the proper tools when troubleshooting.
CAUTION
System integrity could be compromised and operational failures could occur if:
• This equipment is used in a manner other than specified. Operate the
instrument as instructed in the product manuals.
• Installation of software that is not authorized by Beckman Coulter onto your
CytoFLEX workstation. Only operate the CytoFLEX with software authorized
by Beckman Coulter.
• Installation of software that is not an original copyrighted version. Only use
software that is an original copyrighted version to prevent virus
contamination.
CAUTION
If you purchased this product from anyone other than Beckman Coulter or an
authorized Beckman Coulter distributor, and, it is not presently under a Beckman
Coulter service maintenance agreement, Beckman Coulter cannot guarantee that
the product is fitted with the most current mandatory engineering revisions or
that you will receive the most current information bulletins concerning the
product. If you purchased this product from a third party and would like further
information concerning this topic, contact us.

B49006AS xix
Safety Notices
Symbol Explanations
CAUTION
Risk of instrument damage. This device is intended for indoor use only. To avoid
device damage, do not install the instrument outdoors.
WARNING
Risk of personal injury. Safety protection can be impaired if used in a manner not
specified by the manufacturer. To avoid personal injury, use the instrument
according to the manufacturer's instructions only.
Symbol Explanations
Symbol Warning Condition Action
Biohazard Symbol Consider all materials (specimens, reagents, controls,
and monoclonal antibodies) and areas these materials
come into contact with as being potentially infectious.
Wear appropriate barrier protection and follow safe
laboratory procedures when handling any material in
the laboratory.
Caution To indicate that caution is necessary when operating
the device or control close to where the symbol is
placed, or to indicate that the current situation needs
operator awareness or operator action in order to
avoid undesirable consequences.
Caution, Risk of Electric
Shock
To indicate hazards arising from dangerous voltages.
Pinch To warn of a closing motion of mechanical parts of
equipment.

B49006AS
xx
Safety Notices
Symbol Explanations
Recycling Symbol
WEEE Wheeled Bin
Symbol
The symbol of a crossed-out wheeled bin on the
product is required in accordance with the Waste
Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of
this marking on the product indicates:
1. that the device was put on the European Market
after August 13, 2005 and
2. that the device is not to be disposed via the
municipal waste collection system of any member
state of the European Union.
For products under the requirement of WEEE
directive, please contact your dealer or local
Beckman Coulter office for the proper
decontamination information and take-back
program which will facilitate the proper
collection, treatment, recovery, recycling, and
safe disposal of device.
For the Japan market:
This system is considered an industrial waste, subject
to special controls for infectious waste. Before disposal
of the system, refer to the Waste Disposal and Public
Cleaning Law for compliance procedures.
Laser Radiation The laser radiation symbol indicates that there
can be laser light radiation in the area. Take
precautions to prevent exposure.
RoHS Caution Symbol This label indicates that the electronic
information product contains certain toxic or
hazardous substances. The center number is the
Environmentally Friendly Use Period (EFUP) date,
and indicates the number of calendar years the
product can be in operation. Upon the expiration
of the EFUP, the product must be immediately
recycled. The circling arrows indicate the product
is recyclable. The date code on the label or
product indicates the date of manufacture.
Laser Class I This label indicates that this product is a Class I
Laser product. Take precautions to prevent
exposure.
Symbol Warning Condition Action
C
LA
SS
1 LA
S
ER PR
O
DU
C
CLASS 1 LASER PRODUCT
PRODUIT LASER CLASSE 1