Beckman Coulter J-Lite JLA 16.250 User manual

J-Lite
JLA-16.250
Fixed Angle Rotor
Used In Beckman Coulter J2
and Avanti J Series Centrifuges
J-TB-072JB
June 2018
© 2018 Beckman Coulter, Inc.

2
J-Lite JLA-16.250 Rotor
Trademarks
Beckman Coulter, the stylized logo, and the Beckman Coulter prod-
uct and service marks mentioned herein are trademarks or registered
trademarks of Beckman Coulter, Inc. in the United States and other
countries.
All other trademarks, service marks, products, or services are trade-
marks or registered trademarks of their respective holders.

3
J-Lite JLA-16.250 Rotor
SAFETY NOTICE
This safety notice summarizes information basic to the safe use of the rotor described in this manual.
The international symbol displayed above is a reminder to the user that all safety instructions should be
read and understood before operation or maintenance of this equipment is attempted. When you see the
symbol on other pages of this publication, pay special attention to the safety information presented.
Observance of safety precautions will also help to avoid actions that could damage or adversely affect
the performance of the rotor. This rotor was developed, manufactured, and tested for safety and
reliability as part of a Beckman Coulter centrifuge/rotor system. Its safety or reliability cannot be
assured if used in a non-Beckman Coulter centrifuge or in a Beckman Coulter centrifuge that has been
modified without Beckman Coulter’s approval.
Handle body fluids with care because they can transmit disease. No known test offers
complete assurance that such fluids are free of micro-organisms. Some of the most
virulent—Hepatitis (B and C) viruses, HIV (I–V), atypical mycobacteria, and certain
systemic fungi—further emphasize the need for aerosol protection. Handle other infectious
samples according to good laboratory procedures and methods to prevent spread of disease.
Because spills may generate aerosols, observe proper safety precautions for aerosol contain-
ment. Do not run toxic, pathogenic, or radioactive materials in this rotor without taking
appropriate safety precautions. Biosafe containment should be used when Risk Group II
materials (as identified in the World Health Organization Laboratory Biosafety Manual)
are handled; materials of a higher group require more than one level of protection.
The rotor and accessories are not designed for use with materials capable of developing
flammable or explosive vapors. Do not centrifuge such materials in nor handle or store them
near the centrifuge.
Although rotor components and accessories made by other manufacturers may fit in the
JLA-16.250 rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of
other manufacturers’ components or accessories in the JLA-16.250 rotor may void the rotor
warranty and should be prohibited by your laboratory safety officer. Only the components
and accessories listed in this publication should be used in this rotor.
Make sure that filled containers are loaded symmetrically into the rotor and that opposing
bottles or tubes are filled to the same level with liquid of the same density.
If disassembly reveals evidence of leakage, you should assume that some fluid escaped the
rotor. Apply all appropriate safety and decontamination procedures to the centrifuge and
accessories as required.
Never exceed the maximum rated speed of the rotor and labware in use. Refer to the section
on
RUN SPEEDS .
Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion
begins in scratches and may open fissures in the rotor with continued use.
!
!
!
!
!
!
!
!

*
The critical speed range is the range of speeds over which the rotor shifts so as to rotate about
its center of mass. Passing through the critical speed range is characterized by some vibration.
†
Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified
radius and speed (r
ω
2
) to the standard acceleration of gravity (g) according to the following
formula:
where ris the radius in millimeters,
ω is the angular velocity in radians per second (2 π RPM /
60), and gis the standard acceleration of gravity (9807 mm/s
2
). After substitution:
RCF rω
2
g
---------=
RCF 1.12 rRPM
1000
------------


2
=
4
J-Lite JLA-16.250 FIXED ANGLE ROTOR
SPECIFICATIONS
Maximum speed (Avanti J series centrifuges) . . . . . . . . . . . . . . 16 000 rpm
Maximum speed (J2 series centrifuges) . . . . . . . . . . . . . . . . . . . 14 000 rpm
Critical speed range* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 600 to 800 rpm
Maximum solution density . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 g/mL
Relative Centrifugal Field†at maximum speed
at rmax (134 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 400 ×g
at rav (90 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 800 × g
at rmin (46 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 200 ×g
kfactor at max. speed in Avanti J series centrifuges. . . . . . . . . . . . . . . 1090
kfactor at max. speed in J2 series centrifuges . . . . . . . . . . . . . . . . . . . 1400
Conditions requiring speed reduction . . . . . . . . . . . . . . . . see
RUN SPEEDS
Maximum allowable imbalance of opposing loads. . . . . . . . . . . . . 10 grams
Number of bottle cavities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Available bottles and tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . see Table 3
Tube angle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25°
Nominal tube capacity of largest tube. . . . . . . . . . . . . . . . . . . . . . . . 250 mL
Nominal rotor capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1500 mL
Rotor weight, empty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.3 kg (22.7 lb)
Rotor weight, maximum sample mass . . . . . . . . . . . . . . . . 13.2 kg (29.1 lb)
Approximate acceleration time to maximum speed
(rotor fully loaded)
in an Avanti J centrifuge (to 16 000 rpm) . . . . . . . . . . . . . . . . 3
1
/2min
in a J2 series centrifuge (to 14 000 rpm) . . . . . . . . . . . . . . . . . 4
1
/2 min
Approximate deceleration time from maximum speed
(rotor fully loaded)
in an Avanti J centrifuge (from 16 000 rpm) . . . . . . . . . . . . . . 2
3
/4min
in a J2 series centrifuge (from 14 000 rpm) . . . . . . . . . . . . . . . . . 4 min
Rotor material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . aluminum
Rotor entry code for Avanti J-E centrifuges . . . . . . . . . . . . . . . . . . . . . 16.25
Rotor entry code for
microprocessor-controlled J2 series centrifuges . . . . . . . . . . . . . . . . 14
25°
rmin
Axis of Rotation
rav
rmax
U.S. Patent No. 5,584,790

5
J-Lite JLA-16.250 Rotor
DESCRIPTION
This rotor has been manufactured in a registered ISO 9001 or 13485
facility for use in Beckman Coulter Avanti J and J2 series centrifuges.
The J-Lite JLA-16.250 fixed angle rotor (see Figure 1), rated for
16 000 rpm in Beckman Coulter Avanti J series centrifuges and
14 000 rpm in Beckman Coulter J2 series centrifuges, is designed
to hold up to six 250-mL bottles at a 25-degree angle to the axis of
rotation. The JLA-16.250 rotor develops centrifugal forces that can
efficiently pellet cells from large volumes, or cell particles from tissue
homogenates. Short column methods (such as partially filled tubes)
may also be used to purify large quantities of virus in a cushion
gradient.
The rotor and lid are made of aluminum and are anodized for corro-
sion resistance—the rotor is black and the lid is blue. The tie-down
knob in the rotor lid secures the rotor to the centrifuge drive hub.
O-rings made of Buna N rubber, located in the rotor lid, help to main-
tain atmospheric pressure inside the rotor during centrifugation if the
O-rings are properly lubricated. Pins in the rotor drive hole mate with
grooves in the centrifuge drive hub (older model centrifuges), or with
drive hub teeth (newer model centrifuges), to prevent the rotor from
slipping during acceleration and deceleration.
Figure 1. The JLA-16.250 Fixed Angle Rotor (dual-locking lid shown)
Lid
Small O-Ring
(893503)
Large
O-Ring
(893502)
Rotor
Body
Fluid
Containment
Annulus

6
J-Lite JLA-16.250 Rotor
The dual-locking lid assembly allows the rotor to be loaded into and
removed from the centrifuge with the lid secured. The rotor may be
placed under a safety hood before the lid is secured or removed. The
JLA-16.250 rotor with dual-locking lid was tested
1
to demonstrate
containment of microbiological aerosols under normal operating
conditions of the associated Beckman Coulter centrifuge, when used
and maintained as instructed. The rotor is also available with a single-
locking lid.
The JLA-16.250 rotor has a patented fluid-containment annulus,
located below the O-ring sealing surface. This feature helps to
prevent the escape of liquid into the centrifuge chamber if tubes or
bottles leak during centrifugation.
The JLA-16.250 rotor is warranted for 7 years (see the
WARRANTY ).
PREPARATION AND USE
Specific information about the JLA-16.250 rotor is given here. Use
the Rotors and Tubes for J Series Centrifuges manual (publication
JR-IM), and the centrifuge instruction manual along with this manual
for complete rotor and accessory information.
NOTE
Although rotor components and accessories
made by other manufacturers may fit in the
JLA-16.250 rotor, their safety in this rotor
cannot be ascertained by Beckman Coulter.
Use of other manufacturers’ components in the
JLA-16.250 rotor may void the rotor warranty
and should be prohibited by your laboratory
safety officer. Only the components and
accessories listed in this publication should be
used in this rotor.
1
Validation of microbiological containment was done at an independent third-party testing facility (CAMR, Porton Down, UK, or USAMRIID,
Ft. Detrick, MD, U.S.A.). Improper use or maintenance may affect seal integrity and thus containment.

7
J-Lite JLA-16.250 Rotor
PRERUN SAFETY CHECKS
Read the
SAFETY NOTICE at the front of this manual before using
the rotor.
1. Make sure that the rotor, lid, and all tubes or bottles and accesso-
ries are clean and show no signs of corrosion or cracking.
2. Inspect the rotor drive pins to ensure that they are not damaged
(see instructions on page 20). If the drive pins appear to be
damaged, contact Beckman Coulter Field Service.
3. Verify that the bottles or tubes being used are listed in Table 3.
4. Check the chemical compatibilities of all materials used. (Refer
to Appendix A in Rotors and Tubes, or Chemical Resistances,
publication IN-175).
ROTOR PREPARATION
For runs at other than room temperature, refrigerate or warm the
rotor before the run for fast equilibration.
1. Be sure that the metal threads in the rotor are clean and lightly
lubricated with Spinkote lubricant (306812). Also ensure that lid
O-rings are lightly but evenly coated with silicone vacuum grease
(335148).
2. Load filled containers symmetrically into the rotor. (Refer
to
BOTTLES AND TUBES on page 15 for information about
containers.) If fewer than six bottles or tubes are being run,
they must be arranged symmetrically in the rotor (see Figure 2).
Opposing containers must be filled to the same level with liquid
of the same density.
OPERATION
• Precool the rotor in the centrifuge or in a refrigerator before use—
especially before short runs—to ensure that the rotor reaches the
set temperature. A suggested precooling cycle is a minimum of
30 minutes at 2000 rpm at the required temperature.

8
J-Lite JLA-16.250 Rotor
Figure 2. Typical Examples of Arranging Bottles or Tubes in the Rotor.
Two, three, four, or six containers can be centrifuged per run,
2if they are arranged in the rotor as shown.
• If fluid containment is required, use capped bottles or tubes. It is
strongly recommended that all containers carrying physiological
fluids be capped, and not overfilled, to prevent leakage.
• If you are using an Avanti J series centrifuge, select the
JLA-16.250 rotor.
• If you are using a microprocessor-controlled J2 series centrifuge,
enter rotor code 14. Refer to your centrifuge instruction manual
for additional information. See
BEFORE USING THE JLA-16.250
ROTOR IN A MICROPROCESSOR-CONTROLLED J2 SERIES
CENTRIFUGE
, below, if you are using the rotor for the first time.
BEFORE USING THE JLA-l6.250 ROTOR IN A MICROPROCESSOR-CONTROLLED
J2 SERIES CENTRIFUGE
If you have a microprocessor-controlled Beckman Coulter J2 series
centrifuge (model J2-MI, J2-21M, J2-21M/E, or J2-MC), you will
need to enter the JLA-16.250 rotor identification code (14) and
maximum speed (l4 000 rpm) into the instrument memory before the
first use of the JLA-16.250 rotor in that instrument. Use the following
instructions.
1. On the centrifuge panel, press and hold the [ROTOR] key. While
holding [ROTOR], press [PROG SAVE]. Release both keys. The rotor
display area will show 1 – – . – . This indicates that you are ready
to program the first entry, which is the rotor identification code 14.
Enter 14, again press and hold the [ROTOR] key, press [PROG SAVE],
and then release both keys.

9
J-Lite JLA-16.250 Rotor
2. The rotor display should now show 2 – – . – . You are now ready
to program the second entry, the JLA-16.250 rotor’s maximum
speed in J2 series centrifuges, which is l4 000 rpm. Enter 14, again
press and hold [ROTOR], press [PROG SAVE], and release both keys.
3. The rotor display should now show 3 – – . – . The third entry
programs the temperature compensation for the rotor. It is not
possible, however, to enter a compensation value at this step; you
must enter 0. Entering temperature compensation is discussed
below (see
TEMPERATURE ) since it is programmed differently
and can be entered only after these three steps have been
completed.
4. To complete the entries, press and hold [ROTOR], then press
[ENTER/RECALL], then release both keys. The display should now
return to normal.
If you make an error, or if you wish to review the entered information
press both [ROTOR] and [PROG SAVE] repeatedly to cycle through
levels 1, 2, and 3. The program can only be logged into memory from
level 3, and only when all three entries are complete.
Once this procedure is complete, the microprocessor memory will
retain the information and it will not have to be entered again. Each
time you run the JLA-16.250 rotor, enter code 14 as described in the
centrifuge instruction manual.
INSTALLING THE ROTOR
1. To prevent the rotor from sticking to the centrifuge drive hub,
apply a thin coat of Spinkote lubricant to the rotor drive pins and
to the lid knob threads.
2. Lightly coat the large and small lid O-rings with silicone vacuum
grease.
3. Dual-locking lid only. Place filled tubes into the rotor, then place
the lid on the rotor and turn the daisy knob to the right (clockwise)
until secure. Do not overtighten.
4. Two v-shaped indicator marks on either side of the rotor drive hole
(see Figure 3) indicate orientation of the rotor drive pins, which
are located in the drive hole. To avoid damaging the drive pins as
you install the rotor, use the indicator marks to help you align the
pins with the centrifuge drive spindle hub.
Tie-down Knob
Daisy
Knob
Knob Threads
Dual-Locking Lid

10
J-Lite JLA-16.250 Rotor
5. Once the rotor is properly aligned carefully lower it straight down
onto the hub. Do not drop the rotor onto the hub.
6. Slowly turn the rotor around the drive spindle to make sure that it
is properly seated. In rare cases, the rotor pins can rest on top of
the spindle hub pins or teeth, which prevents the rotor from being
secured to the drive spindle. Turning the rotor after installing it
will move the pins into proper position.
Figure 3. Rotor Top View: Indicator Marks Show Drive Pin Orientation
7. Secure the rotor to the centrifuge drive spindle as follows:
Dual-locking lid: press the lid knob down and turn it to the right
(clockwise) until secure, no more than two full turns. Do not
overtighten.
Single-locking lid: place filled tubes into the rotor, then place the
lid on the rotor. Press the lid knob down and turn it to the right
(clockwise) until secure. Do not overtighten.
WARNING
!
If the rotor is left in the centrifuge between
runs, make sure that the rotor is seated on the
drive hub and that the tie-down knob is tight
before each run.
Tie-down Knob
Knob Threads
Single-Locking Lid
Indicator Marks

11
J-Lite JLA-16.250 Rotor
TEMPERATURE
To ensure that the JLA-16.250 rotor reaches the required temperature
during the run, follow the appropriate instructions below for the
J centrifuge model being used.
Avanti J Series Centrifuges (Except Avanti J-E)
Enter the run temperature according to the instructions in your centri-
fuge instruction manual. No additional input is required.
For Avanti J-E Series Centrifuge
Use the following temperature offset table to set the desired sample
temperature.
Table 1. Temperature Settings for Model J-E High Performance
Centrifuge. At speeds above 13,000 rpm, use the indicated temperature
setting for the desired sample temperature. Interpolate if intermediate values
are required. At some speed and temperature combinations, the centrifuge
may be unable to achieve the desired sample temperature.
“N” indicates that the rotor cannot achieve the required temperature at this speed.
Rotor
Speed
(rpm)
Required Sample Temperature (°C)
–10 –4 2 4 6 8 10 20
16 000
14 000
13 000
N
N
–10
N
–6
–4
N
0
2
N
2
4
N
6
6
6
8
8
8
10
10
20
20
20

12
J-Lite JLA-16.250 Rotor
J2 Series Centrifuges
Enter the required run temperature and the appropriate temperature
compensation units (see Tables 1 and 2) on the centrifuge control
panel following the instructions below.
Table 2. Temperature Compensation Settings for Analog
and Microprocessor-Controlled Centrifuges (Models J2-HS,
J2-21, J2-MI, J2-21M, J2-MC, and J2-21M/E).
Interpolate if intermediate values are required.
“N” indicates that the rotor cannot achieve the required temperature at this speed.
Table 3. Temperature Compensation Settings for the Model J2-HC
Analog Centrifuge. Interpolate if intermediate values are required.
“N” indicates that the rotor cannot achieve the required temperature at this speed.
Analog J2 Series Centrifuges (Models J2-HS, J2-21, and J2-HC)
1. Turn the SET knob on the centrifuge control panel to the required
sample temperature.
2. Find the compensation value in Table 1 or Table 2 that corre-
sponds to the required temperature and run speed. Set the COMP
dial to that setting. (Interpolate if intermediate values are
required.)
Rotor
Speed
(rpm)
Required Sample Temperature (°C)
–10 2 5 10 20 40
14 000
10 000
5 000
N
–4
0
–7
–3
0
–5
–3
0
–4
–2
0
–3
–1
0
–3
N
N
Rotor
Speed
(rpm)
Required Sample Temperature (°C)
–10 2 5 10 20 40
14 000
10 000
5 000
N
–4
0
–7
–3
0
–7
–3
–3
–7
–2
–3
–5
–1
–3
–4
N
N

13
J-Lite JLA-16.250 Rotor
Microprocessor-Controlled J2 Series Centrifuges (Models J2-MI,
J2-21M, J2-MC, and J2-21M/E)
Enter rotor code 14, then follow the steps below.
1. Press the [TEMP] key on the centrifuge control panel and then use
the keypad to enter the sample temperature.
2. Find the compensation value in Table 1 that corresponds with the
set temperature and run speed.
3. Press [COMP ADJ]. The word “COMP” flashes below the
TEMPERATURE display and the display flashes.
4. Use the keypad to enter the desired compensation value. Press the
[±.] key to enter a minus sign; pressing it again will remove the
minus sign.
5. Check the temperature display. (If the entry is incorrect, press [CE]
and reenter the digits.)
6. When the entry is correct, press [ENTER/RECALL].
NOTE
To clear a COMP ADJ entry, press [COMP ADJ],
[0], and [ENTER/RECALL].
RUN PROCEDURE
WARNING
!
Operator error or tube failure may generate
aerosols. Toxic, pathogenic, or other
hazardous materials must not be run in
this rotor unless all appropriate safety
precautions are taken. Also see the Safety
Notice at the beginning of this manual.

14
J-Lite JLA-16.250 Rotor
!
CAUTION
Make sure that the rotor is properly seated on
and securely fastened to the drive hub before
each run.
When the rotor is properly loaded and installed in the centrifuge,
you are ready to perform the run. Refer to the applicable centrifuge
instruction manual for operating instructions.
NOTE
Line voltage fluctuations can cause variations in
acceleration and deceleration times.
REMOVAL AND SAMPLE RECOVERY
!CAUTION
If disassembly reveals evidence of leakage, you
should assume that some fluid escaped the rotor.
Apply appropriate decontamination procedures
to the centrifuge and accessories, as required.
Dual-Locking Lid
1. Place one hand on the rotor to keep it from turning. Grasp the
tie-down knob with your other hand and turn it to the left (counter-
clockwise), while pulling up, until the stem disengages from the
drive hub.
2. Place your hands on the rotor and lift the rotor straight up off the
drive hub. Do not lift the rotor by the tie-down knob.
3. Place the rotor on a counter or bench top under a safety hood
(if required).

15
J-Lite JLA-16.250 Rotor
4. Grasp the daisy knob and turn it to the left (counterclockwise)
until it disengages.
5. Remove the lid and set it aside. If the lid cannot be removed, first
pull up on the tie-down knob to release vacuum inside the rotor,
and then remove the lid.
!CAUTION
Always loosen the round tie-down knob to
disengage the rotor from the centrifuge drive
hub before attempting to loosen the daisy knob.
The dual-locking lid assembly can be damaged if
the daisy knob is loosened while the rotor is still
engaged with the hub.
Single-Locking Lid
1. Unscrew the tie-down knob to release the rotor from the drive hub.
2. Remove the lid and set it aside.
3. Lift the rotor straight up off the drive hub. Do not lift the rotor by
the tie-down knob.
NOTE
Lubricating the centrifuge drive hub with
Spinkote should prevent the rotor from sticking
in J2 series centrifuges. In Avanti J series centri-
fuges, the drive hubs do not need lubrication
because Delrin rings on the hubs prevent
sticking.
BOTTLES AND TUBES
The JLA-16.250 rotor uses the bottles and tubes listed in Table 3.
Be sure to use only those items listed and observe the maximum
fill volumes and speed limits shown. (Maximum fill volume is the
maximum amount of fluid that can be centrifuged in the listed

16
J-Lite JLA-16.250 Rotor
container.) To minimize the possibility of leakage from capped tubes or
bottles, load tubes or bottles with sample, secure the caps, and precool
the loaded, sealed labware to run temperature before beginning the run.
Refer to Appendix A in Rotors and Tubes for information on the chem-
ical resistances of tube and accessory materials.
Temperature Limits
• Plastic containers have been tested for use at temperatures between 2
and 25°C. For centrifugation at other temperatures, pretest
containers under anticipated run conditions.
• If plastic containers are frozen before use, make sure that they are
thawed to at least 2°C before centrifugation.

17
J-Lite JLA-16.250 Rotor
Table 4. Available Bottles and Tubes for the JLA-16.250 Rotor.
Use only the items listed here and observe fill volumes and maximum run speeds.
a
Maximum speeds listed are guidelines only. These speeds have been achieved in reliability tests at Beckman Coulter, but because of
manufacturing variances no guarantee of performance or fit is expressed or implied. (The maximum rotor speed in J2 series centrifuges is
14 000 rpm.)
b
Polypropylene.
c
Commercially available. Observe manufacturer’s maximum speed and fill guidelines.
d
Delrin. Delrin is a registered trademark of E.I. DuPont de Nemours & Company.
Dimensions
and Volume
Tube
Description
Part
Number
Max.
Fill
Volume
(mL)
Required Accessory
Max
Speed
a
/
RCF/
kFactor
Description
Part
Number
Tubes
per
Adapter
62 ×120 mm
250 mL
polypropylene bottle
w/cap assembly
356011 250 none 16 000 rpm
38 500 ×g
1 070
62 ×120 mm
250 mL
polycarbonate bottle
w/cap assembly
356013 250 none 16 000 rpm
38 500 ×g
1 090
29 ×104 mm
50 mL
polypropylene bottle
w/cap assembly
357001 40 adapter 356997
b
1 16 000 rpm
32 200 ×g
650
29 ×104 mm
50 mL
polycarbonate bottle
w/cap assembly
357000 40 adapter 356997 1 16 000 rpm
32 400 ×g
660
29 ×104 mm
50 mL
polycarbonate bottle,
screw-on cap
357002 38.5 adapter 356997 1 16 000 rpm
32 200 ×g
660
29 ×104 mm
50 mL
polypropylene bottle,
screw-on cap
357003 38.5 adapter 356997 1 16 000 rpm
32 200 ×g
650
29 ×104 mm
50 mL
thickwall polycarbonate,
snap-on cap
363664 36 adapter 356997 1 16 000 rpm
32 100 ×g
400
29 ×104 mm
50 mL
thickwall polypropylene,
snap-on cap
357005 36 adapter 356997 1 16 000 rpm
32 100 ×g
400
17 ×119 mm
15 mL
conical tube
c
15 adapter 356964
b
4
c
Bio-vials
4 mL
polypropylene 566353 4 adapter 342098
d
9 4 000 rpm
2 300 ×g
10 700

18
J-Lite JLA-16.250 Rotor
Thickwall Tubes
Thickwall polypropylene and polycarbonate tubes can be run partially
filled (at least half filled) with or without caps, but all opposing tubes
for a run must be filled to the same level with liquid of the same den-
sity. Do not overfill capless tubes; be sure to note the fill volumes and
run speeds shown in Table 3.
Polycarbonate and Polypropylene Bottles
Capped polycarbonate and polypropylene bottles may be centrifuged
completely or partially filled (not less than half full). Again, all
opposing containers for a run must be filled to the same level.
RUN TIMES
The kfactor is a measure of a rotor’s pelleting efficiency. Beckman
Coulter has calculated the kfactor for all of its rotors, at maximum
speed with full bottles or tubes, using the following formula:
(1)
Where ωis the angular velocity of the rotor in radians per second
(ω= 0.105 ×rpm), r
max
is the maximum radius, and r
min
is the
minimum radius.
After substitution:
(2)
Use the kfactor in the following equation to estimate the run time t
(in hours) required to pellet particles of known sedimentation coeffi-
cient s(in Svedberg units, S).
kr
max
r
min
⁄()ln
ω
2
-------------------------------------10
13
3600
------------×=
k2.533 10
11
×()r
max
r⁄
min
()ln
rpm
2
--------------------------------------------------------------------------=

19
J-Lite JLA-16.250 Rotor
(3)
For centrifugation below maximum speed, estimate the run time
required by adjusting the kfactor as follows:
(4)
Run times can also be estimated from data established in prior
experiments using a different rotor if the kfactor of the previous rotor
is known. For any two rotors, a and b:
(5)
Where the kfactors have been adjusted for the actual run speed used.
For more information on kfactors, see Use of kFactor for
Estimating Run Times from Previously Established Run Conditions
(publication DS-719).
RUN SPEEDS
The centrifugal force at a given radius in a rotor is a function of
run speed. Comparisons of forces between different rotors are made
by comparing the rotors’ relative centrifugal fields (RCF). When
rotational speed is adjusted so that identical samples are subjected
to the same RCF in two different rotors, the samples are subjected
to the same force. The RCF at a number of rotor speeds is provided
in Table 4.
tk
s
--=
k
adj
kmaximum speed
actual run speed
-----------------------------------------


2
=
t
a
t
b
-----
k
a
k
b
------=
SPEED

20
J-Lite JLA-16.250 Rotor
Table 5. Relative Centrifugal Fields for the JLA-16.250 Rotor.
Entries in this table are calculated from the formula
RCF = 1.12r (RPM/1000)
2
and are then rounded to three significant digits.
Rotor
Speed
(rpm)
Relative Centrifugal Field (×g)
at r
max
(134 mm)
at r
av
(90 mm)
at r
min
(46 mm)
16 000
15 000
14 000
13 000
12 000
38 400
33 800
29 400
25 400
21 600
25 800
22 700
19 800
17 000
14 500
13 200
11 600
10 100
8 710
7 420
11 000
10 000
9 000
8 000
18 200
15 000
12 200
9 610
12 200
10 100
8 160
6 450
6 230
5 150
4 170
3 300
7 000
6 000
5 000
4 000
7 350
5 400
3 750
2 400
4 940
3 630
2 520
1 610
2 520
1 860
1 290
824
3 000
2 000
1 000
500
1 350
600
150
38
907
403
100
25
464
206
52
13
0
5 000
10 000
15 000
20 000
25 000
30 000
35 000
40 000
0 4 000 8 000 12 000 16 000
rmax
rmin
rav
Relative Centrifugal Fields for the JLA-16.250 Rotor
Speed (rpm)
RCF (x g)
2 000 6 000 10 000 14 000