Beckman Coulter COULTER EPICS XL User manual

COULTER®EPICS®XL™Flow Cytometer
COULTER®EPICS®XL-MCL™Flow Cytometer
Service Manual
PN 4237029F (October 2002)
Beckman Coulter, Inc.
Fullerton, CA 92835
..
Epics XL • MCL
Coulter

WARNINGS AND PRECAUTIONS
Beckman Coulter, Inc. makes no representation that, upon furnishing this service manual, the holder of the manual will have
the necessary technical capabilities and know-how to properly troubleshoot and repair any of the equipment specified in the
manual. Beckman Coulter, Inc. assumes no liability whatsoever, including consequential and incidental damages, resulting
from improper operation of Beckman Coulter instruments after maintenance of Beckman Coulter instruments has been
performed by persons not employed by Beckman Coulter, Inc. Furthermore, Beckman Coulter, Inc. assumes no liability
whatsoever for any personal injury or property damage resulting from maintenance and/or repair of Beckman Coulter
instruments performed by persons not employed by Beckman Coulter, Inc.
READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL BEFORE ATTEMPTING
TO OPERATE INSTRUMENT.
Beckman Coulter, Inc. urges its customers to comply with all national health and safety standards such as the use of barrier
protection. This may include, but it is not limited to, protective eyewear, gloves, and suitable laboratory attire when
operating or maintaining this or any other automated laboratory analyzer.
"This Service Manual contains confidential information of Beckman Coulter, Inc. and its receipt or possession does not
convey any rights to reproduce, disclose its contents, or to manufacture, use, or sell anything it may describe.
Reproduction, disclosure, or use without specific written authorization of Beckman Coulter, Inc. is strictly forbidden."
Copyright © Beckman Coulter, Inc. 1993 - 2002
All rights reserved.
HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS
WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:
WARNING - Can cause injury.
CAUTION - Can cause damage to the instrument.
IMPORTANT - Can cause misleading results.

iii
PN 4237029F
REVISION STATUS
Initial Issue, 7/93
Software Version 1.0.
Revision B, 6/94
Software Version 1.5.
Added fluidic schematics, electronic block diagrams, and procedures, and updated parts for MCL. Pages
changed or added: i, iii to vii, 1-1, 2-6 to 2-19, 4-14 to 4-35, 5-1, 5,7, 5-8, 8-1 to 8-6, 8-11 to 8-16.
Revision C, 10/94
Software Version 1.5.
Corrected text on pages 4-15, 4-21 and 4.22. Updated the PMI procedure on pages 5-7 and 5-8. Added to
and corrected the parts list, pages 8-1 to 8-24. Updated Figures 2.9 and 2.10.
Revision D, 3/98
Released by CN 306660-0561
Software Version 2.1.
Complete revision to update manual to software version 2.1 and reformat manual to new numbering
system and style.
Revision E, 9/00
Released by CN 312810-0012
Software Versions: System II version 3.0
EXPO32 version 1.0
EXPO32ADC version 1.1
Revision of Parts List and IPL based on new logos, bezels, covers, and panels. Check digits for all part
numbers were deleted. Updated illustrations for title page and cytometer in Figure 8.2-6.
Revision F, 10/2002
Released by CN 306660-0797
Software Versions:
SYSTEM II™ version 3.0
Prefinal version F
EXPO32™ ADC version 1.1
Complete revision that includes information released by Service Memos 1664, 1722, 1776, 1982, 3045,
3061, 3066, 3067, 3095, 3131 and Technical Updates TU2000M027 and TU2000M084.
This document applies to the latest software listed and higher versions. When a subsequent software version affects the
information in this document, the changes will be included on minor revision change pages or summarized on a Notice of
Information Update form and will be released by service memo.

PN 4237029F
iv
REVISION STATUS

PN 4237029F v
CONTENTS
WARNINGS AND PRECAUTIONS
REVISION STATUS, iii
CONTENTS,v
1 INTRODUCTION,1.1-1
1.1 MANUAL DESCRIPTION,1.1-1
Scope,1.1-1
Notification of Updates,1.1-1
Intended Audience,1.1-1
Organization,1.1-2
Numbering Format,1.1-3
Special Headings,1.1-3
WARNING,1.1-4
CAUTION,1.1-4
IMPORTANT,1.1-4
ATTENTION,1.1-4
Note,1.1-4
Conventions,1.1-4
1.2 SAFETY PRECAUTIONS,1.2-1
Special,1.2-1
Electronic,1.2-1
Biological,1.2-2
Laser,1.2-2
Laser Beam,1.2-2
Radiation Hazards,1.2-3
Troubleshooting,1.2-7
2 INSTRUMENT DESCRIPTION,2.1-1
2.1 SYSTEM OVERVIEW,2.1-1
Function,2.1-1
Sheath Flow,2.1-1
Sample Flow,2.1-1
Segmenting Valve/Positions Flow,2.1-1
Components,2.1-1
Cytometer (All Signal Processing Electronics),2.1-2
Power Supply Module,2.1-2
Computer Workstation,2.1-3
MCL Option - Theory of Operation,2.1-3
2.2 CYTOMETER,2.2-1
Optics,2.2-2
Laser,2.2-2
Flow Cell,2.2-2
Pinhole Assembly,2.2-3
Forward Scatter (FS) Detector,2.2-4
Laser Intersection,2.2-4
Signal Processing,2.2-4

PN 4237029F
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CONTENTS
Data Acquisition Card Cage,2.2-4
Amplifier (Amp)/Signal Conditioner Card,2.2-6
Trans Data Acquisition Card,2.2-8
System Interface Card,2.2-9
Cyto Transputer Card,2.2-10
MCL Interface Card,2.2-11
Optical Transprocessor Extended Memory Cards,2.2-12
Opto Transprocessor EXMEM Card,2.2-12
Opto Transprocessor EXMEM II Card,2.2-12
Transputers,2.2-12
Fiber Optic Interface Card,2.2-14
Sensor Card,2.2-14
Solenoid Power Distribution Card,2.2-14
2.3 POWER SUPPLY MODULE,2.3-1
Description,2.3-1
Components,2.3-1
Linear Power Supply,2.3-4
2.4 WORKSTATION,2.4-1
Components,2.4-1
Standard Configuration,2.4-1
Options,2.4-2
2.5 NETWORKING,2.5-1
Purpose,2.5-1
Introduction,2.5-1
Topolo gies,2.5-2
Mesh,2.5-2
Star,2.5-2
Linear Bus,2.5-3
Ring,2.5-4
Bridges, Repeaters, Routers,2.5-4
Specifications,2.5-5
Dedicated vs. Non-Dedicated Server,2.5-5
LANtastic™ Network Operating System,2.5-5
3 INSTALLATION PROCEDURES,3.1-1
PART A: INSTRUMENT INSTALLATION
3.1 PRE-SITE INSPECTION,3.1-1
Space and Accessibility,3.1-1
Power Requirements,3.1-2
Optional Computer Peripherals,3.1-3
Environment,3.1-3
Unloading and Moving the Instrument,3.1-3
Installation Assistance,3.1-4
Inspection Report,3.1-4

PN 4237029F vii
CONTENTS
3.2 PREINSTALLATION CHECKS,3.2-1
Pre-Site Inspection Compliance,3.2-1
Education Center Training,3.2-1
Condition of Cartons Received,3.2-1
Optional Computer Peripherals,3.2-1
Supplies,3.2-1
3.3 INITIAL INSTALLATION,3.3-1
Tools/Supplies Needed,3.3-1
Unpacking,3.3-1
Power Supply Module,3.3-1
Cytometer,3.3-1
Power Supply Installation,3.3-2
Cytometer Installation,3.3-3
Remove Covers to Provide Needed Access,3.3-3
Remove the Optical Bench Shipping Tie-Down in the Front,3.3-8
Remove the Optical Bench Shipping Tie-Down in the Back (Behind the
Laser),3.3-9
Prepare Components inside the Lower Pneumatics Drawer for
Operation,3.3-12
Reinstall the MCL CPU Card (if removed earlier),3.3-12
Prepare the Data Acquisition Card Cage for Operation,3.3-12
Prepare the Segmenting Valve for Operation,3.3-13
Prepare Components inside the Upper Pneumatic Drawer for
Operation,3.3-14
Fill the Reagent Containers,3.3-15
Fill the Sheath Container,3.3-15
Fill the Cleaning Agent Container,3.3-16
3.4 WORKSTATION INSTALLATION,3.4-1
Initial Setup,3.4-1
Mouse Connection,3.4-2
Keyboard Connection,3.4-2
Monitor Connections - Overview,3.4-2
Monitor Connections: 17-inch Flat Panel Thin Film Transistor (TFT)
Monitor,3.4-2
Connecting the Monitor Cables,3.4-2
Monitor Connections: 22-inch CRT Monitor,3.4-3
Connecting the Monitor Cables,3.4-3
Printer Option,3.4-3
3.5 CABLE/TUBING CONNECTIONS,3.5-1
Connect Cables,3.5-1
Laser Umbilical Cable,3.5-1
Logic Cable,3.5-3
Analog Cable,3.5-3
Power Module Control Cable,3.5-3
Make Appropriate MCL Connections,3.5-3
Fiber Optic Interface Cables,3.5-4
CYT12 Cable,3.5-5

PN 4237029F
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CONTENTS
Power Cords,3.5-5
Connect Tubings,3.5-5
Prepare the Waste Container for Tubing Connection,3.5-5
Tubing Connections,3.5-6
3.6 SOFTWARE INSTALLATION,3.6-1
Tools/Supplies Needed,3.6-1
SYSTEM II Software Installation,3.6-1
3.7 OPERATIONAL CHECKS,3.7-1
Tools/Supplies Needed,3.7-1
Preliminary Operational Checks,3.7-1
Purge Air Bubbles from the Sheath Filter,3.7-1
Initial System Verification,3.7-3
Power Supply Verification,3.7-3
Trans Data Acquisition Card Offset Verification,3.7-3
Functional Verification,3.7-3
Reinstall Covers and Panels,3.7-4
Lower the Data Acquisition Card Cage,3.7-4
Upper Pneumatic Drawer,3.7-4
Front Covers,3.7-4
Right-Side Cover,3.7-5
Left-Side Cover,3.7-5
Top Cove r,3.7-5
Power Supply Cover,3.7-5
Clean Up,3.7-6
Complete the System Verification Procedure (SVP),3.7-6
PART B: UPGRADES AND OPTIONS INSTALLATION
3.8 MCL OPTION UPGRADE,3.8-1
Purpose,3.8-1
Tools/Supplies Needed,3.8-1
Preparation,3.8-1
Cytometer Upgrade,3.8-1
Power Supply Module Upgrade,3.8-2
Power Supply Voltage Verification and Adjustment,3.8-3
MCL Alignment,3.8-3
Operational Verification,3.8-3
Reinstall Covers,3.8-3
System Verification,3.8-4
3.9 4-COLOR PMT UPGRADE,3.9-1
Purpose,3.9-1
Tools/Supplies Needed,3.9-1
Preparation,3.9-1
Hardware Installation,3.9-1
Amp/Signal Conditioner Card Calibration,3.9-3
PMT Operational Alignment,3.9-3
Reinstall Covers,3.9-3
System Verification,3.9-3

PN 4237029F ix
CONTENTS
3.10 FlowCentre™II MULTIMEDIA WORKSTATION UPGRADE,3.10-1
Purpose,3.10-1
Tools/Supplies Needed,3.10-1
Preparation,3.10-1
Opto Transprocessor EXMEM or EXMEM II Card Retrieval and Installation,3.10-1
Mouse Connection,3.10-1
Keyboard Connection,3.10-2
Monitor Connection (New or Existing),3.10-2
Cable Connections,3.10-3
Fiber Optic Interface Cables,3.10-3
CYT12 Cable,3.10-4
Power Cord,3.10-4
SYSTEM II Software Installation,3.10-4
EXPO32™ Software Option Installation,3.10-5
System Verification,3.10-5
3.11 BAR-CODE PRINTER OPTION,3.11-1
Purpose,3.11-1
Tools/Supplies Needed,3.11-1
Printer Setup,3.11-1
Media Calibration,3.11-3
XL-MCL Flow Cytometer Connection,3.11-4
Printer Testing (through SYSTEM II Software),3.11-4
Label Tear-Off Position Adjustment, 3.11-4
Printer Thermal Head Adjustment,3.11-5
Top-of-Label Position Adjustment,3.11-5
Ribbon Replacement,3.11-5
3.12 BAR-CODE HAND-HELD SCANNER OPTION,3.12-1
Purpose,3.12-1
Tools/Supplies Needed,3.12-1
Installation,3.12-1
Verification,3.12-2
3.13 SERIAL/PARALLEL ADAPTER INTERFACE CARD OPTION,3.13-1
Purpose,3.13-1
Tools/Supplies Needed,3.13-1
Removal,3.13-1
Installation,3.13-1
Verification,3.13-2
3.14 NETWORK SETUP USING MICROSOFT WINDOWS 95 OR 98,3.14-1
Purpose,3.14-1
Tools/Supplies Needed,3.14-1
Software Installation,3.14-1
Cable Connections,3.14-2
Set Up Hard Drives and Printers,3.14-2
Define the Computer Name and Workgroup,3.14-3
Establish Control,3.14-3
Map a Network Shared Drive, if desired,3.14-3

PN 4237029F
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CONTENTS
3.15 LANtastic®CLIENT/SERVER SOFTWARE OPTION, VERSION 8.0,3.15-1
Purpose,3.15-1
Tools/Supplies Needed,3.15-1
Preparation,3.15-1
Hardware Installation,3.15-1
CD-ROM Usage in DOS,3.15-1
Software Installation, Client Setup,3.15-1
Software Installation, Server Setup,3.15-5
Software and Hardware Validation,3.15-5
3.16 LANtastic®CLIENT/SERVER SOFTWARE OPTION, VERSION 7.0,3.16-1
Purpose,3.16-1
Tools/Supplies Needed,3.16-1
LANtastic Client/Server Version for Windows™ 95 Installation,3.16-1
LANtastic Client Version for MS-DOS Installation,3.16-2
Sybase Client/Server SQL Software Option,3.16-3
3.17 LANtastic®CLIENT/SERVER SOFTWARE OPTION, VERSION 6.0,3.17-1
Purpose,3.17-1
Tools/Supplies Needed,3.17-1
Installation,3.17-1
Config.sys and startnet.bat File Editing,3.17-2
LANtastic File Server Software Connection,3.17-2
Sybase Client/Server SQL Software Option,3.17-3
3.18 Sybase®CLIENT/SERVER SQL SOFTWARE OPTION,3.18-1
Purpose,3.18-1
Tools/Supplies Needed,3.18-1
Preparation,3.18-1
Software Installation, Client Setup,3.18-1
Software Installation, Server Setup,3.18-2
Software and Hardware Validation,3.18-3
3.19 EXPO32™ SOFTWARE OPTIONS,3.19-1
Purpose,3.19-1
Tools/Supplies Needed,3.19-1
Preparation,3.19-1
For EXPO32 ADC Installation Option,3.19-1
For Printer Installation,3.19-1
Software Installation,3.19-1
Verification,3.19-1
4 SERVICE AND REPAIR PROCEDURES,4.1-1
4.1 GUIDELINES FOR SERVICING THE INSTRUMENT,4.1-1
General Guidelines,4.1-1
Safety Precautions,4.1-1
Instrument Access,4.1-1
Laser Safety,4.1-1
Electronic Precautions,4.1-1

PN 4237029F xi
CONTENTS
Procedures,4.1-2
Tools and Supplies,4.1-3
Instrument Performance Verification,4.1-3
Power Down / Power Up Using SYSTEM II™ Software,4.1-3
Power Down Using SYSTEM II Software,4.1-3
Stage 1: Power Off the Cytometer (system using SYSTEM II software),4.1-3
Stage 2: Power Off the Workstation (system using SYSTEM II software),4.1-4
Stage 3: System Power Down (system using SYSTEM II software),4.1-4
Power Up Using SYSTEM II Software,4.1-4
Stage 1: Restoring Power to the System (system using SYSTEM II
software),4.1-4
Stage 2: Restoring Power to the Workstation (system using SYSTEM II
software),4.1-4
Restoring Power to the Cytometer Only (system using SYSTEM II
software),4.1-5
Power Down / Power Up Using EXPO32™ ADC Software,4.1-6
Power Down Using EXPO32 ADC Software,4.1-6
Stage 1: Power Off the Cytometer (system using EXPO32 ADC
software),4.1-6
Stage 2: Power Off the Workstation (system using EXPO32 ADC
software),4.1-6
Stage 3: System Power Down (system using EXPO32 ADC software),4.1-7
Power Up Using EXPO32 ADC Software,4.1-7
Stage 1: Restoring Power to the System (system using EXPO32 ADC
software),4.1-7
Stage 2: Restoring Power to the Workstation (system using EXPO32 ADC
software),4.1-7
Restoring Power to the Cytometer Only (system using EXPO32 ADC
software),4.1-8
4.2 PREFINAL SERVICE SOFTWARE INSTALLATION AND OPERATION,4.2-1
Purpose,4.2-1
Tools/Supplies Needed,4.2-1
Preparation,4.2-1
If Using SYSTEM II™ Software,4.2-1
If Using EXPO32™ ADC Software,4.2-2
Installation,4.2-2
Operation,4.2-3
Wrap Up,4.2-3
If SYSTEM II is the Customer’s Preferred Operating System,4.2-3
If EXPO32 ADC is the Customer’s Preferred Operating System,4.2-4
4.3 COVER REMOVAL AND REINSTALLATION,4.3-1
Purpose,4.3-1
Overview,4.3-1
Locating a Procedure,4.3-1
Exterior Cytometer Covers,4.3-2
Access Inside the Cytometer,4.3-3
Power Supply Module Covers,4.3-4

PN 4237029F
xii
CONTENTS
Procedure 1, 4.3-5
How to Remove and Reinstall the Cytometer Top Cover,4.3-5
Tools/Supplies Needed,4.3-5
Removing the Cytometer Top Cover,4.3-5
Cytometer Interlock Switch,4.3-6
Reinstalling the Cytometer Top Cover,4.3-6
Procedure 2, 4.3-7
How to Remove and Reinstall the Cytometer Right-Side Cover,4.3-7
Tools/Supplies Needed,4.3-7
Removing the Cytometer Right-Side Cover,4.3-7
Reinstalling the Cytometer Right-Side Cover,4.3-8
Procedure 3, 4.3-9
How to Remove and Reinstall the Center Front Cover (Filter Cover),4.3-9
Tools/Supplies Needed,4.3-9
Removing the Center Front Cover (Filter Cover),4.3-9
Reinstalling the Center Front Cover (Filter Cover),4.3-9
Procedure 4, 4.3-10
How to Properly Open and Close the Cytometer Reagent Drawer,4.3-10
Tools/Supplies Needed,4.3-10
Opening the Reagent Drawer,4.3-10
Closing the Reagent Drawer,4.3-12
Procedure 5, 4.3-13
How to Remove and Reinstall the Cytometer Upper Rear Cover,4.3-13
Tools/Supplies Needed,4.3-13
Removing the Cytometer Upper Rear Cover,4.3-13
Reinstalling the Cytometer Upper Rear Cover,4.3-14
Procedure 6, 4.3-15
How to Remove and Reinstall the Cytometer Lower Rear Cover,4.3-15
Tools/Supplies Needed,4.3-15
Removing the Cytometer Lower Rear Cover,4.3-15
Reinstalling the Cytometer Lower Rear Cover,4.3-16
Procedure 7, 4.3-17
How to Remove and Reinstall the Manual Sample Station, Cytometer with MCL
Option,4.3-17
Tools/Supplies Needed,4.3-17
Removing the Manual Sample Station on a Cytometer with the MCL Option
Installed,4.3-17
Reinstalling the Manual Sample Station on a Cytometer with the MCL Option
Installed,4.3-19
Procedure 8, 4.3-20
How to Remove and Reinstall the Manual Sample Station, Cytometer without MCL
Option,4.3-20
Tools/Supplies Needed,4.3-20
Removing the Manual Sample Station on a Cytometer without the MCL
Option,4.3-20
Reinstalling the Manual Sample Station on a Cytometer without the MCL
Option,4.3-22

PN 4237029F xiii
CONTENTS
Procedure 9, 4.3-23
How to Open and Close the MCL Door,4.3-23
Tools/Supplies Needed,4.3-23
Opening the MCL Door,4.3-23
MCL Interlock Switch,4.3-23
Closing the MCL Door,4.3-24
Procedure 10, 4.3-25
How to Unlatch and Relatch the MCL Covers,4.3-25
Tools/Supplies Needed,4.3-25
Unlatching the MCL Covers,4.3-25
Relatching the MCL Covers,4.3-26
Procedure 11, 4.3-27
How to Remove and Reinstall MCL Covers,4.3-27
Tools/Supplies Needed,4.3-27
Removing the MCL Covers,4.3-27
Reinstalling the MCL Covers,4.3-29
Procedure 12, 4.3-30
How to Remove and Reinstall the MCL Probe Housing,4.3-30
Tools/Supplies Needed,4.3-30
Removing the MCL Probe Housing Cover,4.3-31
Reinstalling the MCL Probe Housing Cover,4.3-33
Procedure 13, 4.3-34
How to Remove and Reinstall the Cytometer Left-Side Cover
(Cytometer with MCL Option Installed),4.3-34
Tools/Supplies Needed,4.3-34
Removing the Cytometer Left-Side Cover (Cytometer with MCL Option
Installed),4.3-34
Reinstalling the Cytometer Left-Side Cover (Cytometer with MCL Option
Installed),4.3-35
Procedure 14, 4.3-36
How to Remove and Reinstall the Cytometer Left-Side Cover
(Cytometer without MCL Option),4.3-36
Tools/Supplies Needed,4.3-36
Removing the Cytometer Left-Side Cover (Cytometer without MCL
Option),4.3-36
Reinstalling the Cytometer Left-Side Cover (Cytometer without MCL
Option),4.3-37
Procedure 15, 4.3-38
How to Remove and Reinstall the Filter Shield,4.3-38
Tools/Supplies Needed,4.3-38
Removing the Filter Shield,4.3-38
Reinstalling the Filter Shield,4.3-41
Procedure 16, 4.3-43
How to Remove and Reinstall the Data Acquisition Card Cage,4.3-43
Tools/Supplies Needed,4.3-43
Removing the Data Acquisition Card Cage from the Cytometer Center
Cavity,4.3-43
Reinstalling the Data Acquisition Card Cage Back in the Cytometer Center
Cavity,4.3-45

PN 4237029F
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CONTENTS
Procedure 17, 4.3-46
How to Remove and Reinstall the Lower Pneumatics Drawer,4.3-46
Tools/Supplies Needed,4.3-46
Removing the Lower Pneumatics Drawer on a Cytometer with the MCL
Option Installed,4.3-46
Removing the Lower Pneumatics Drawer on a Cytometer without the MCL
Option,4.3-49
Reinstalling the Lower Pneumatics Drawer,4.3-50
Procedure 18, 4.3-52
How to Remove and Reinstall the Upper Pneumatics Drawer EMC Shield,4.3-52
Tools/Supplies Needed,4.3-52
Removing the Upper Pneumatics Drawer EMC Shield,4.3-52
Reinstalling the Upper Pneumatics Drawer EMC Shield,4.3-53
Procedure 19, 4.3-54
How to Remove and Reinstall the Power Supply Module Three-Side Cover,4.3-54
Tools/Supplies Needed,4.3-54
Removing the Power Supply Module’s Three-Sided Cover,4.3-54
Interlock Switch for the Power Supply Module,4.3-54
Reinstalling the Power Supply Module’s Three-Sided Cover,4.3-55
Procedure 20, 4.3-56
How to Open and Close the Power Supply Module Front Door,4.3-56
Tools/Supplies Needed,4.3-56
Open the Front Door,4.3-56
Close the Front Door,4.3-56
4.4 ARGON LASER HEAD OR POWER SUPPLY REPLACEMENT,4.4-1
Purpose,4.4-1
Tools/Supplied Needed,4.4-1
Preparation,4.4-1
Laser Power Supply,4.4-1
Removal,4.4-1
Installation,4.4-2
Laser Head,4.4-2
Removal,4.4-2
Installation,4.4-3
Wrap Up,4.4-3
4.5 FLOW CELL AND BEAMSHAPER REPLACEMENT AND/OR ALIGNMENT,4.5-1
Purpose,4.5-1
Tools/Supplies Needed,4.5-1
Preparation,4.5-1
Flow Cell Replacement,4.5-2
Beamshaper Assembly Removal, Replacement, and Alignment,4.5-3
Install Alignment Tools,4.5-4
Alignment,4.5-4
Reinstall Covers,4.5-6
System Verification,4.5-6

PN 4237029F xv
CONTENTS
4.6 OPTICAL ALIGNMENT,4.6-1
Purpose,4.6-1
Tools/Supplies Needed,4.6-1
Preparation,4.6-1
Laser Beam Steering,4.6-1
Forward Scatter (FS) Mask Alignment,4.6-2
How to Proceed,4.6-4
4.7 SAMPLE AND SHEATH SENSOR CALIBRATION,4.7-1
Purpose,4.7-1
Tools/Supplies Needed,4.7-1
Preparation,4.7-1
Preliminary Voltage Checks,4.7-1
Calibration,4.7-2
Operational Verification,4.7-4
Reinstall Assemblies and Covers,4.7-5
System Verification,4.7-5
4.8 CARRYOVER TEST,4.8-1
Purpose,4.8-1
Tools/Supplies Needed,4.8-1
Preparation,4.8-1
Procedure,4.8-2
Using the Manual Sample Station,4.8-2
Using the MCL Sample Station,4.8-2
4.9 PEEK TUBING REPLACEMENT,4.9-1
Purpose,4.9-1
Tools/Supplies Needed,4.9-1
Preparation,4.9-1
Removal,4.9-1
Installation,4.9-3
Reinstall Covers,4.9-5
System Verification,4.9-5
4.10 SHEATH PRESSURE/SAMPLE STATION VACUUM CALIBRATION,4.10-1
Purpose,4.10-1
Tools/Supplies Needed,4.10-1
Preparation,4.10-1
Calibration,4.10-1
Verification,4.10-1
Reinstall Covers,4.10-1
System Verification,4.10-1
4.11 SEGMENTING VALVE CLEANING,4.11-1
Purpose,4.11-1
Tools/Supplies Needed,4.11-1
Preparation,4.11-1

PN 4237029F
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CONTENTS
Removal,4.11-1
Front Segmenting Pad,4.11-1
Middle Segmenting Pad,4.11-2
Rear Segmenting Pad,4.11-2
Clean the Shaft,4.11-3
Installation,4.11-3
Rear Segmenting Pad,4.11-3
Middle Segmenting Pad,4.11-3
Front Segmenting Pad,4.11-4
Complete the Installation,4.11-4
Reinstall Covers,4.11-4
Verification,4.11-4
4.12 MCL OPTION FIELD ADJUSTMENT,4.12-1
Purpose,4.12-1
Tools/Supplies Needed,4.12-1
Preparation,4.12-1
MCL TERMINAL Program Setup,4.12-1
Carousel Tube-Position Sensor Adjustment,4.12-2
Finger Adjustment,4.12-3
MCL Sample Probe Adjustment, 4.12-4
How to Proceed,4.12-4
4.13 VORTEXER FOOT ASSEMBLY REPLACEMENT,4.13-1
Purpose,4.13-1
Tools/Supplies Needed,4.13-1
Preparation,4.13-1
Removal,4.13-1
Installation,4.13-2
Reinstall Covers,4.13-3
Verification,4.13-3
4.14 TRANS DATA ACQUISITION CARD REPLACEMENT AND/OR
CALIBRATION,4.14-1
Purpose,4.14-1
Tools/Supplies Needed,4.14-1
Preparation,4.14-1
If Replacing the Trans Data Acquisition Card,4.14-1
If Calibrating Without Replacing the Trans Data Acquisition Card,4.14-2
Access the Prefinal Menu,4.14-2
Calibration, 4.14-2
Reinstall Cover,4.14-4
Load the Customer’s Preferred Operating System,4.14-4
System Verification,4.14-4
4.15 AMP/SIGNAL CONDITIONER CARD REPLACEMENT AND/OR
CALIBRATION,4.15-1
Purpose,4.15-1
Tools/Supplies Needed,4.15-1
If Replacing the Amp/Signal Conditioner Card,4.15-1

PN 4237029F xvii
CONTENTS
If Calibrating Without Replacing the Amp/Signal Conditioner Card,4.15-2
Calibration,4.15-2
Verify Acceptable Histograms,4.15-4
Reinstall Cover,4.15-6
System Verification,4.15-6
4.16 PREPARING INSTRUMENT FOR SHIPMENT,4.16-1
Purpose,4.16-1
Tools/Supplies Needed,4.16-1
Procedure,4.16-1
4.17 INSTRUMENT SHUTDOWN,4.17-1
Purpose,4.17-1
Tools/Supplies Needed,4.17-1
Procedure,4.17-1
4.18 THREE-WIRE CIRCUIT ANALYZER TEST,4.18-1
Purpose,4.18-1
Tools/Supplies Needed,4.18-1
Procedure,4.18-1
Reverse/Shorted Ground/Neutral Test,4.18-2
Tools/Supplies Needed,4.18-2
Procedure,4.18-2
4.19 POWER SUPPLY REPLACEMENT,4.19-1
Purpose,4.19-1
Tools/Supplies Needed,4.19-1
Preparation,4.19-1
Removal,4.19-2
Replacement,4.19-2
System Verification,4.19-2
4.20 POWER SUPPLY VOLTAGE VERIFICATION AND ADJUSTMENT,4.20-1
Purpose,4.20-1
Tools/Supplies Needed,4.20-1
+24 Vdc Power Supply,4.20-1
Preparation,4.20-1
Voltage Verification,4.20-2
Voltage Adjustment,4.20-3
+5 Vdc Power Supply,4.20-4
Preparation,4.20-4
Voltage Verification,4.20-4
Voltage Adjustment,4.20-4
±15 Vdc Power Supply,4.20-4
Preparation,4.20-4
+15 Vdc Voltage Verification,4.20-5
-15 Vdc Voltage Verification,4.20-5
Voltage Adjustment,4.20-5

PN 4237029F
xviii
CONTENTS
MCL Power Supply,4.20-6
Preparation,4.20-6
Voltage Verification,4.20-6
Voltage Adjustment,4.20-7
4.21 RE-IMAGING FlowCentre™ COMPUTERS,4.21-1
Purpose,4.21-1
Tools/Supplies Needed,4.21-1
Preparation,4.21-1
Re-imaging a FlowCentre II Tower Computer,4.21-1
Re-imaging a FlowCentre Desktop Computer without a Cache Controller
Card,4.21-1
Re-imaging a FlowCentre Desktop Computer with a Cache Controller Card,4.21-2
4.22 UNIVERSAL CD-ROM DRIVER SETUP,4.22-1
Purpose,4.22-1
Tools/Supplies Needed,4.22-1
Preparation,4.22-1
Driver Installation,4.22-1
4.23 BAR-CODE SCANNER EEPROM CUSTOM PROGRAMMING,4.23-1
Purpose,4.23-1
Tools/Supplies Needed,4.23-1
Preparation,4.23-1
Computer Setup,4.23-2
Custom Programming Sequence, 4.23-3
If You Want to Store this Custom Configuration (Optional),4.23-4
If You Do Not Want to Store this Custom Configuration,4.23-4
Reinstall Covers,4.23-5
Verification,4.23-5
5 MAINTENANCE PROCEDURES,5.1-1
5.1 SYSTEM VERIFICATION PROCEDURE (SVP),5.1-1
Purpose,5.1-1
Required Service Form,5.1-1
Tools/Supplies Needed,5.1-1
Preparation,5.1-1
Static Tests,5.1-1
Power Up Tests,5.1-2
Trans Data Acquisition Card Offset Verification,5.1-2
Acquisition, CV Analysis and Carryover Check,5.1-2
Using the Manual Sample Station,5.1-2
Using the MCL Sample Station,5.1-4
Interlock Checks,5.1-5
Workstation Computer Test,5.1-6
On-Site Close Out,5.1-6

PN 4237029F xix
CONTENTS
5.2 PREVENTATIVE MAINTENANCE INSPECTION (PMI),5.2-1
Purpose,5.2-1
Tools/Supplies Needed,5.2-1
Procedure,5.2-1
System Verification,5.2-2
6SCHEMATICS,6.1-1
6.1 ENGINEERING SCHEMATICS,6.1-1
7 TROUBLESHOOTING,7.1-1
7.1 LEVEL SENSE INDICATORS AND ERROR MESSAGES,7.1-1
Level Sense Indicators,7.1-1
Cleanse Low,7.1-1
Sheath Low and Waste Full,7.1-1
Error Messages,7.1-1
About the Error Messages Table,7.1-2
7.2 PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL,7.2-1
Purpose,7.2-1
Tools/Supplies Needed,7.2-1
Preparation,7.2-1
ADC Zero Adjust,7.2-1
Purpose,7.2-1
Circuit Card Checked in this Test,7.2-2
Response if Test Fails,7.2-2
Amp Gain Control,7.2-2
Purpose,7.2-2
Circuit Cards Checked in this Test,7.2-2
Response if Test Fails,7.2-2
Amp Saturation Test,7.2-2
Purpose,7.2-2
Circuit Cards Checked in this Test,7.2-2
Response if Test Fails,7.2-2
Attenuator Control,7.2-3
Purpose,7.2-3
Circuit Cards Checked in this Test,7.2-3
Response if Test Fails,7.2-3
Beeper Test,7.2-3
Purpose,7.2-3
Circuit Card Checked in this Test,7.2-3
Response if Test Fails,7.2-3
Canyon Jumper Test,7.2-4
Purpose,7.2-4
Circuit Cards Checked in this Test,7.2-4
Response if Test Fails,7.2-4
Count Rate Test,7.2-4
Purpose,7.2-4
Circuit Card Checked in this Test,7.2-4
Response if Test Fails,7.2-4

PN 4237029F
xx
CONTENTS
DMA Acquisition - Not Used,7.2-4
Front Panel Test,7.2-4
Purpose,7.2-4
Circuit Cards Checked in this Test,7.2-5
Response if Test Fails,7.2-5
General Information,7.2-5
Grand Canyon Adjust,7.2-6
Purpose,7.2-6
Circuit Cards Checked in this Test,7.2-6
Response if Test Fails,7.2-6
Grand Canyon Test,7.2-6
Purpose,7.2-6
Circuit Cards Checked in this Test,7.2-6
Response if Test Fails,7.2-6
Histogram Test,7.2-7
Purpose,7.2-7
Initialize System,7.2-7
Purpose,7.2-7
Circuit Cards Checked in this Test,7.2-7
Response if Test Fails,7.2-7
Laser Control,7.2-8
Purpose,7.2-8
Options,7.2-8
Components Checked in this Test,7.2-8
Response if Test Fails,7.2-8
Laser Warm Up,7.2-9
Purpose,7.2-9
Lock Up Test - Not Used,7.2-9
MCL Bar Code Head Test,7.2-9
Purpose,7.2-9
MCL Bar Code Test,7.2-9
Purpose,7.2-9
MCL Burn In,7.2-10
Purpose,7.2-10
Error Index Numbers,7.2-10
MCL Carousel Label,7.2-10
Purpose,7.2-10
MCL Carousel Status,7.2-11
Purpose,7.2-11
Test Sequence,7.2-11
Response if Test Fails,7.2-11
MCL Door Switch Test,7.2-11
Purpose,7.2-11
Response if Test Fails,7.2-11
MCL Finger Test,7.2-11
Purpose,7.2-11
MCL Home Align,7.2-11
Purpose,7.2-11
Summary of Test Sequence,7.2-11