Beckman Coulter JA-14.50 User manual

PN B32164AC
October 2016
Instructions For Use
JA-14.50 Fixed-Angle Rotor
For Use in Beckman Coulter Avanti J Series
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

JA-14.50 Fixed-Angle Rotor
PN B32164AC (October 2016)
© 2016 Beckman Coulter, Inc.
All rights reserved.
Beckman Coulter, the stylized logo, and the Beckman
Coulter product and service marks mentioned herein are
trademarks or registered trademarks of Beckman Coulter,
Inc. in the United States and other countries.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Contact Us
If you have any questions, contact our Customer Support
Center.
• Worldwide, find us via our website at
www.beckmancoulter.com/customersupport/support.
• In the USA and Canada, call us at 1-800-369-0333.
• Outside of the USA and Canada, contact your local
Beckman Coulter Representative.”
Original Instructions

PN B32164AC iii
Revision History
For labeling updates, go to www.beckmancoulter.com and download the latest version of the manual or
system help for your instrument.
Issue AC, 07/2016 — Updates were made to the following sections: Safety Information for the JA-14.50
Rotor, Specifications, Description, Preparation and Use, Tubes, Replacement Rotor Parts, Supplies.
Note: Changes that are part of the most recent revision are indicated in text by a bar in the margin of the
amended page.

PN B32164AC
iv
Revision History

PN B32164AC v
Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to use this equipment. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to
how to proceed in any situation, contact your Beckman Coulter Representative.
Alerts for Warning, Caution, and Note
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.
NOTE
NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.

PN B32164AC
vi
Safety Notice
Safety Information for the JA-14.50 Rotor
Safety Information for the JA-14.50 Rotor
This rotor was developed, manufactured, and tested for safety and reliability as part of a
Beckman Coulter centrifuge/rotor system. Its safety or reliability cannot be assured if used in a
centrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter centrifuge that has been
modified without Beckman Coulter’s approval.
Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that such fluids are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) viruses, HIV (I-V), atypical mycobacteria, and certain systemic fungi—further emphasize the
need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe
proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive
materials in this rotor without taking appropriate safety precautions. Biosafe containment should
be used when Risk Group II materials (as identified in the World Health Organization Laboratory
Biosafety Manual) are handled; materials of a higher group require more than one level of
protection.
The rotor and accessories are not designed for use with materials capable of developing flammable
or explosive vapors, or a hazardous chemical reaction. Do not centrifuge such materials in nor
handle or store them near the centrifuge.
Although rotor components and accessories made by other manufacturers may fit in the JA-14.50
rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other
manufacturers’ components or accessories in the JA-14.50 rotor, excluding the tubes referenced in
Table 1 and Table 3 may void the rotor warranty and should be prohibited by your laboratory safety
officer. Only the components and accessories listed in this publication should be used in this rotor.
Do not run an empty rotor. Place filled tubes in at least two opposing cavities. Make sure that filled
containers are loaded symmetrically into the rotor and that opposing tubes are filled to the same
level with liquid of the same density.
If disassembly reveals evidence of leakage, and pathogenic or radioactive materials are involved,
you should assume that some fluid escaped the rotor. Apply appropriate decontamination
procedures to the centrifuge and accessories.
Never exceed the maximum rated speed of the rotor and labware in use. Refer to the section on Run
Speeds, and derate the run speed as appropriate.
Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins
in scratches and may open fissures in the rotor with continued use.

vii
Contents
Revision History,iii
Safety Notice,v
Alerts for Warning, Caution, and Note,v
Safety Information for the JA-14.50 Rotor,vi
JA-14.50
Fixed-Angle Rotor,1
Specifications,1
Description,2
Preparation and Use,4
Prerun Safety Checks,4
Rotor Preparation,4
Operation,6
Installing the Rotor,6
Rotor Drive Pin Orientation,6
Rotor Lid Configurations,8
Rotor Installation,9
Run Procedure,10
Removal and Sample Recovery,10
Dual-Locking Lid,10
Single-Locking Lid,11
If the Rotor Sticks to the Drive Hub,12
Tubes,13
Tubes for JA-14.50 Rotors Manufactured before October 2016,13
Tubes for JA-14.50 Rotors Manufactured after October 2016,16
Temperature Limits,17
Run Times,18
Run Speeds,20
Care and Maintenance,21
Maintenance,21
Cleaning,22
Decontamination,23
Sterilization and Disinfection,24
Storage,24
Returning a Rotor,25
Supply List,25
Replacement Rotor Parts,25
Supplies,26

viii
Contents
Beckman Coulter, Inc.
J Series Rotor Warranty

ix
Illustrations
Illustrations
1TheJA-14.50FixedAngleRotor,2
2 Fluid-Containment Annulus,3
3 Typical Examples of Arranging Sixteen or Fewer Tubes in the
Rotor,5
4 Rotor Drive Pin Location and Orientation,7
5 Centrifuge Drive Hub Configurations,7
6 Installing the Rotor,8
7 Using the Rotor Removal Tool,12
8 Rotor Serial Number with T,13
9 Rotor Lid Serial Number with T,13
10 Examples of Tubes with Minimum and Moderate Crazing,18
11 Relative Centrifugal Fields Chart for the JA-14.50 Rotor,21

x
Tables
Tables
1 Adapters to support tubes from other manufacturers for the JA-
14.50 Rotor manufactured before October 2016,13
2 Available Beckman Coulter Bottles and Tubes for the JA-14.50
Rotor manufactured prior to October 2016,14
3 Adapters to support tubes from other manufacturers for the JA-
14.50 Rotor manufactured after October 2016,16
4 Available Beckman Coulter Bottles and Tubes for the JA-14.50
Rotor manufactured after October 2016 ,17
5 Relative Centrifugal Fields for the JA-14.50 Rotor,20

PN B32164AC 1
JA-14.50
Fixed-Angle Rotor
Specifications
NOTE At 14,000 RPM and at an ambient temperature of 25°C or below, the minimum rotor temperature is
4°C. Above 38°C ambient, 4°C may not be achievable at 14,000 RPM.
1. Axis of Rotation
Maximum speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14,000 RPM
Critical speed range
a
. . . . . . . . . . . . . . . . . . . . . . . . . . . .400 to 1000 RPM
Density rating at maximum speed. . . . . . . . . . . . . . . . . . . . . . . 1.2 g/mL
Relative Centrifugal Field
b
at maximum speed
at r
max
(160 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35,000 g
at r
av
(124 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27,100 g
at r
min
(87 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19,100 g
kfactors at maximum speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 787
Conditions requiring speed reductions . . . . . . . . . . . . . see Run Speeds
Number of tube cavities. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Available tubes see Table 1, Table 2, Table 3 and Table 4
Nominal dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 115 mm
Nominal tube capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 mL
Nominal rotor capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 800 mL
Approximate acceleration time to maximum
speed (rotor fully loaded) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
½ min
Approximate deceleration time from maximum
speed (rotor fully loaded, max. break) . . . . . . . . . . . . . . . . . 2
½ min
Rotor weight, unloaded . . . . . . . . . . . . . . . . . . . . . . . 10.5 kg (23 lbs 3 oz.)
Rotor material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . aluminum
a. The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass. Passing through the critical
speed range is characterized by some vibration.
b. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (r
2
) to the standard acceleration of
gravity (g) according to the following formula: RCF = r
2
/g— where ris the radius in millimeters, is the angular velocity in radians per second
(2 RPM /60), and gis the standard acceleration of gravity (9807 mm/s
2
). After substitution: RCF = 1.12r(RPM/1000)
2

PN B32164AC
2
JA-14.50 Fixed-Angle Rotor
Description
Description
Beckman Coulter JA-14.50 rotors are manufactured in a facility that maintains certifications to both
ISO 9001:2008 and ISO 13485:2003. They are for use with the specified Beckman Coulter centrifuges.
The JA-14.50 fixed-angle rotor is designed to hold up to sixteen 50-mL tubes at a 35-degree angle
(see Figure 1). Used in Beckman Coulter Avanti J series centrifuges, the JA-14.50 rotor develops
centrifugal forces that can efficiently pellet cells, bacteria, and food products; separate proteins,
viruses, and subcellular fractions, and do phase separation and binding studies.
The JA-14.50 rotor with dual-locking lid was tested
*
to demonstrate containment of microbiological
aerosols under normal operating conditions of the associated Beckman Coulter centrifuge, when
used and maintained as instructed. The rotor is also available with a single-locking lid.
Figure 1 The JA-14.50 Fixed Angle Rotor
The JA-14.50 rotor has a patented fluid-containment annulus, located below the O-ring sealing
surface (see Figure 2). The annulus helps to retain liquid that may escape from leaking or overfilled
tubes, thereby minimizing the escape of liquid into the instrument chamber during centrifugation.
1. Dual-Locking Rotor Lid
2. Small O-ring
3. Large O-ring
4. Rotor Body
* Validation of microbiological containment was done at an independent third-party testing facility. Improper use or
maintenance may affect seal integrity and thus containment.
1. Single-Locking Lid
2. Rotor Body
3. O-Ring
4. Dual-Locking Lid

PN B32164AC 3
JA-14.50 Fixed-Angle Rotor
Description
Figure 2 Fluid-Containment Annulus
The rotor and lid are made of anodized aluminum. Two O-rings, one in the rotor lid made of Buna-N
rubber, and one in the rotor body made of ethylene propylene rubber, help to maintain atmospheric
pressure inside the rotor during centrifugation when they are properly lubricated. Pins in the rotor
drive hole mate with the centrifuge drive hub to prevent the rotor from slipping during acceleration
and deceleration.
JA-14.50 rotors manufactured after October 2016 have a slightly tapered cell profile, which improves
performance of most commercially available 50 mL conical tubes. However, some tubes and
adapters that were compatible prior to October 2016 are no longer compatible. Please consult Tables
1-4 for compatible tubes and adapters.
NOTE
JA14.50 rotors and lids manufactured after October 2016 include the letter “T” after the serial number
(see Figure 8 and Figure 9).
See the Warranty at the back of this manual for warranty information.
1. Annulus

PN B32164AC
4
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Preparation and Use
Specific information about the JA-14.50 rotor is given here. Use the Rotors and Tubes for
Beckman Coulter J Series Centrifuges manual (publication JR-IM-10) and the centrifuge instruction
manual along with this manual for complete rotor and accessory operation information.
NOTE
Although rotor components and accessories made by other manufacturers may fit in the JA-14.50
rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’
components or accessories in the JA-14.50 rotor, excluding the tubes referenced in Ta b l e 1 and Ta b l e 3 ,
may void the rotor warranty and should be prohibited by your laboratory safety officer. Only the
components and accessories listed in this publication should be used in this rotor.
Prerun Safety Checks
Read the Safety Notice section at the front of this manual before using the rotor.
1 Make sure that the rotor, lid, and all tubes or accessories are clean and show no signs of
corrosion or cracking.
2 Verify that the tubes being used are listed in Table 1, Table 2, Table 3 and Table 4.
3 Refer to Chemical Resistances (publication IN-175), included in the Rotors and Tubes CD-ROM for
the chemical compatibilities of all materials used.
Rotor Preparation
For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration.
1 Be sure that metal threads in the rotor are clean and lightly but evenly lubricated with Spinkote
lubricant (306812).
Also ensure that O-rings are lightly but evenly coated with silicone vacuum grease (335148).
2 Load the filled containers symmetrically into the rotor.
• (Refer to Tubes for information about containers.)
• If fewer than sixteen tubes are being run they must be arranged symmetrically in the rotor
(see Figure 3).
• Opposing tubes must be filled to the same level with liquid of the same density.

PN B32164AC 5
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Figure 3 Typical Examples of Arranging Sixteen or Fewer Tubes in the Rotor

PN B32164AC
6
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Operation
1 Precool the rotor in the centrifuge or in a refrigerator before use—especially before short
runs—to ensure that the rotor reaches the set temperature.
A suggested precooling cycle is a minimum of 30 minutes at 2000 RPM at the required
temperature.
2 If fluid containment is required, use capped tubes.
It is strongly recommended that all containers carrying physiological fluids be capped to
prevent leakage.
3 Select the JA-14.50 rotor.
4 For additional operating information, see the following:
• Run Times for using kfactors to adjust run durations.
• Run Speeds for information about speed limitations.
Installing the Rotor
Rotor Drive Pin Orientation
Two metal pins inside the rotor drive hole engage with teeth on the centrifuge drive hub to prevent
the rotor from slipping during acceleration and deceleration (see Figure 4). When the rotor is
properly installed, the pins sit either in the drive hub grooves or on top of the hub surface (Figure 5).
The rotor pins are positioned parallel to the Beckman Coulter name engraved at the center of the
rotor body (see Figure 6). The markings on the lifting ring, located near the top periphery of the
rotor, also are indicators of the drive pin position. Knowing the pin orientation before you install
the rotor will help to ensure that you position the rotor properly on the hub, minimizing the chance
of hub damage. Follow the steps below.

PN B32164AC 7
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Figure 4 Rotor Drive Pin Location and Orientation
Figure 5 Centrifuge Drive Hub Configurations
1. Rotor Drive Hole
2. Drive Pins
3. Avanti J Centrifuge
Drive Spindle Hub
1. Drive Hub Grooves (4)
2. Rotor Pins (2)
3. Drive Hub
4. Drive Spindle Assembly
5. Older Model Centrifuges
Be sure the pins in the rotor drive hole
are seated in the grooves of the drive
spindle hub as shown
6. Te e t h ( 2 )
7. Drive Hub
8. Drive Spindle Assembly
9. Newer Model Centrifuges
Be sure the pins in the rotor drive
hole are not sitting on top of the
teeth on the drive spindle hub
16
4
3
2
7
8
59

PN B32164AC
8
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Figure 6 Installing the Rotor
Rotor Lid Configurations
The rotor has two available lid configurations.
• The dual-locking lid (B19947) allows the lid to be attached to and removed from the rotor
outside the centrifuge, so that tubes can be loaded and unloaded under a safety hood. The dual-
locking lid consists of a daisy knob that attaches the lid to the rotor body, and a tie-down knob
that attaches the rotor to the centrifuge drive hub.
1. To p V i e w 2. Side View
1. Tie-down Knob
2. Daisy Knob
3. Lubricate Threads
4. Dual-Locking Lid
1
4
3
2

PN B32164AC 9
JA-14.50 Fixed-Angle Rotor
Preparation and Use
• The single-locking lid (B19952) has a tie-down knob that secures the rotor to the centrifuge
drive spindle. The rotor must be placed in the centrifuge before the lid is attached.
Rotor Installation
1 To prevent the rotor from sticking to the centrifuge drive hub, apply a thin coat of Spinkote
lubricant to the rotor drive pins and to the lid knob threads.
2 Lightly coat the large and small O-rings with silicone vacuum grease.
3 Dual-locking lid only. Place filled tubes into the rotor, then place the lid on the rotor and turn the
daisy knob to the right (clockwise) until secure.
Do not overtighten.
4 Turn the rotor until the Beckman Coulter name or the engraved drive pin alignment indicators
are either horizontal or perpendicular to the drive hub pins or teeth (see Figure 4).
Or, rotate the drive spindle to align the hub pins or teeth with the rotor drive pins.
5 Carefully lower the rotor straight down onto the centrifuge drive spindle hub.
Do not drop the rotor onto the spindle hub.
6 Slowly turn the rotor around the drive spindle to make sure that it is properly seated.
In rare cases, the rotor pins can rest on top of the spindle hub pins or teeth, which prevents the
rotor from being secured to the drive spindle.
Turning the rotor after installing it will move the pins into proper position.
1. Tie-down Knob
2. Lubricate Threads
3. Single-Locking Lid
1
3
2

PN B32164AC
10
JA-14.50 Fixed-Angle Rotor
Preparation and Use
7 Secure the rotor to the centrifuge drive spindle as follows:
a. Dual-locking lid: press the lid knob down and turn it to the right (clockwise) until secure,
no more than two full turns.
Do not overtighten.
b. Single-locking lid: place filled tubes into the rotor, then place the lid on the rotor.
Press the lid knob down and turn it to the right (clockwise) until secure.
Do not overtighten.
CAUTION
If the rotor is left in the centrifuge between runs, make sure that the rotor is
seated on the drive hub and that the tie-down knob is tight before each run.
Run Procedure
When the rotor is properly loaded and installed in the centrifuge, you are ready to perform the run.
Refer to the applicable centrifuge instruction manual for operating instructions.
WARNING
Operator error or tube failure may generate aerosols. Toxic, pathogenic, or other
hazardous materials must not be run in this rotor unless all appropriate safety
precautions are taken. Also see the Safety Notice at the beginning of this manual.
CAUTION
Make sure that the rotor is properly seated on and securely fastened to the drive
hub before each run.
Removal and Sample Recovery
CAUTION
If disassembly reveals evidence of leakage, you should assume that some fluid
escaped the rotor. Apply all appropriate safety and decontamination procedures
to the centrifuge and accessories.
Dual-Locking Lid
1 Place one hand on the rotor to keep it from turning.