Beckman Coulter CytoFLEX Series User manual

B49006AH
March 2017
Instructions for Use
CytoFLEX Series
CytoFLEX, CytoFLEX S, and CytoFLEX LX Flow Cytometers
For Research Use Only. Not for use in diagnostic procedures.
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

CytoFLEX Series
Flow Cytometer
PN B49006AH (March2017)
© 2017 Beckman Coulter, Inc.
All rights reserved.
Beckman Coulter, the stylized logo, and the Beckman
Coulter product and service marks mentioned herein are
trademarks or registered trademarks of Beckman Coulter,
Inc. in the United States and other countries.
Windows 7, Windows 8, and Windows 10 are registered
trademarks or trademarks of Microsoft Corporation in the
United States and/or other countries.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Contact Us
If you have any questions, contact our Customer Support
Center.
• Worldwide, find us via our website at
www.beckmancoulter.com/customersupport/support.
• In the USA and Canada, call us at 1-800-369-0333.
• Outside of the USA and Canada, contact your local
Beckman Coulter Representative.
Beckman Coulter Eurocenter S.A.
22, rue Juste-Olivier
Case Postale 1044
CH - 1260 Nyon 1, Switzerland
Tel: [phone]

B49006AH iii
Revision History
Initial Issue AA, 09/2014
Software Version 1.0
Issue AB, 12/2014
Software Version 1.0
Updates were made to the following sections: Symbol Explanations, Figure 1.10, Figure 1.11, Figure 9.3,
Figure 9.5, Figure 9.6, Figure 9.7, Disposal Of Electrical Instrumentation, Lifting and Carrying Instructions,
Replacing the Sheath Fluid Harness and/or Waste Harness, and Installing the Instrument and Connecting the
Equipment.
Issue AC, 02/2015
Software Version 1.1
Updates were made to the following sections:
Safety Notices
Symbol Explanations
CHAPTER 1, System Overview
Optical Fiber
Fluidics Module
Plate Loader Components
System Configuration
Instrument Specifications
Performance Characteristics
CHAPTER 2, Using the CytExpert Software
Start Page
Acquisition Screen
Test Tubes
Plot area
Status Bar
Analysis Screen
Compensation Experiment Screen
QC Experiment Screen
Software Menu
Graphic and Gating Styles
Software Settings
CHAPTER 3, Daily Startup
Opening the Software
Selecting the Plate Loader Sample Injection Mode [With Plate Loader]
Running the System Startup Program
Running the System Startup Program [With Plate Loader]
This document applies to the latest software listed and higher versions. When a subsequent software version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckmancoulter.com and download the latest version of the manual or system help for your instrument.

B49006AH
iv
Revision History
CHAPTER 4, Instrument Quality Control
Preparing the QC Sample [With Plate Loader]
Collecting QC Data
Confirming Results
Collecting QC Data [With Plate Loader]
Creating Levey-Jennings Charts
QC Result Manager
CHAPTER 5, Data Acquisition and Sample Analysis
Creating an Experiment
Creating an Experiment [With Plate Loader]
Sampling and Collecting Data
Setting the Channel and Label
Adjusting the Threshold
Creating Plots and Gates
Setting Customized Parameters
Setting Custom Statistics
Analyzing and Exporting Data
Exporting FCS Files
Exporting Plots or the Statistics Table of Multiple Tubes as Picture Files
Printing Graphics
CHAPTER 6, Compensation
Creating a Compensation Experiment
Creating a Compensation Experiment [With Plate Loader]
Manually Adjusting Compensation
Managing the Compensation Library
CHAPTER 7, Data Review
Calculating Sample Injection Volume and Concentration
Importing Previously Acquired Data
CHAPTER 9, Troubleshooting
Plate Loader Hazard Labels and Location
Table 9.2, Troubleshooting [With Plate Loader]
CHAPTER 10, Cleaning Procedures
Daily Clean [With Plate Loader]
Preparing the Instrument for Transport or Storage
This document applies to the latest software listed and higher versions. When a subsequent software version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckmancoulter.com and download the latest version of the manual or system help for your instrument.

B49006AH v
Revision History
CHAPTER 11, Replacement/Adjustment Procedures
Adding the Deep Clean Solution
Replacing the Sample Probe Assembly [With Plate Loader]
Changing the Sample Probe from the Single Tube Sample Station to the Plate Loader [With Plate Loader]
Changing the Sample Probe from the Plate Loader to the Single Tube Sample Station [With Plate Loader]
Replacing the Plate Holder [With Plate Loader]
Plate Loader Module Removal and Reinstallation [With Plate Loader]
Calibrating the Sample Flow Rate
Calibrating the Sample Flow Rate [With Plate Loader]
Changing Sample Mixing and Backflush Settings
Calibrating the Plate Position [With Plate Loader]
APPENDIX A, Instrument Installation
Power Source
Unpacking the Instrument and Inspecting the Materials for Defects or Omissions
Installing the CytExpert Software
Issue AD, 04/2015
Software Version 1.1
Updates were made to the following sections:
CHAPTER 1, System Overview
Cytometer
CHAPTER 2, Using the CytExpert Software
Analysis Screen
CHAPTER 8, Daily Shutdown
Shutting Down the Instrument
CHAPTER 11, Replacement/Adjustment Procedures
Changing the Event Rate Setting
Calibrating the Plate Position [With Plate Loader]
Issue AE, 11/2015
Software Version 1.1
Updates were made to the following sections:
CHAPTER 1, System Overview
Plate Loader Components
Plate Holder Components
Dimensions [CytoFLEX]
Acoustic Noise Level
Performance Characteristics
CHAPTER 4, Instrument Quality Control
Confirming Results
This document applies to the latest software listed and higher versions. When a subsequent software version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckmancoulter.com and download the latest version of the manual or system help for your instrument.

B49006AH
vi
Revision History
CHAPTER 9, Troubleshooting
Plate Loader Hazard Labels and Location
CHAPTER 10, Cleaning Procedures
Daily Clean
Daily Clean [With Plate Loader]
Preparing the Instrument for Transport or Storage
CHAPTER 11, Replacement/Adjustment Procedures
Replacing the Sample Probe Assembly [With Plate Loader]
Replacing the Plate Holder [With Plate Loader]
Plate Loader Module Removal and Reinstallation [With Plate Loader]
APPENDIX A, Instrument Installation
Installing the Instrument and Connecting the Equipment
Issue AF, 10/2016
Software Version 1.1
Updates were made to the following sections:
Safety Notices
Safety Precautions
Introduction
About this Manual
CHAPTER 11, Replacement/Adjustment Procedures
Changing the Sample Probe from the Single Tube Sample Station to the Plate Loader [With Plate Loader]
Changing the Sample Probe from the Plate Loader to the Single Tube Sample Station [With Plate Loader]
CytoFLEX Plate Loader Upgrade Kit
CytoFLEX Plate Loader Upgrade Kit Components
Changing the Sample Probe from the Single Tube Sample Station to the Plate Loader [With CytoFLEX Plate
Loader Upgrade Kit]
Changing the Sample Probe from the Plate Loader to the Single Tube Sample Station [With CytoFLEX Plate
Loader Upgrade Kit]
Issue AG, 1/2017
Software Version 2.0
Complete Revision
Issue AH, 3/2017
Software Version 2.0
Updates were made to the following sections:
Introduction
About this Manual
This document applies to the latest software listed and higher versions. When a subsequent software version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckmancoulter.com and download the latest version of the manual or system help for your instrument.

B49006AH vii
Revision History
CHAPTER 1, System Overview
Performance Characteristics [CytoFLEX]
Performance Characteristics [CytoFLEX LX]
CHAPTER 3, Daily Startup
Selecting the Plate Loader Sample Injection Mode [With Plate Loader]
CHAPTER 11, Replacement/Adjustment Procedures
Managing the Maintenance Reminder
Replacing the Sample Probe and/or the Sample Peristaltic Pump Tubing
Replacing the Sample Probe Assembly [With Plate Loader]
Changing the Sample Probe from the Single Tube Sample Station to the Plate Loader [CytoFLEX With Plate
Loader]
Changing the Sample Probe from the Plate Loader to the Single Tube Sample Station [CytoFLEX With Plate
Loader]
Changing Sample Mixing and Backflush Settings
APPENDIX A, Instrument Installation
Installing the Software [CytoFLEX]
Upgrading the CytExpert Software
Reinstalling the CytExpert Software
Added the following Chapter:
APPENDIX C, Sample Injection Mode Control Kit
This document applies to the latest software listed and higher versions. When a subsequent software version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckmancoulter.com and download the latest version of the manual or system help for your instrument.

B49006AH
viii
Revision History

B49006AH ix
Safety Notices
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to
how to proceed in any situation, contact us.
Beckman Coulter, Inc. urges its customers to comply with all national health and safety standards
such as the use of barrier protection. This may include, but it is not limited to, protective eyewear,
gloves, and suitable laboratory attire when operating or maintaining this or any other automated
laboratory analyzer.
This manual assumes that users have basic knowledge of the Windows operating system, as well as
experience working with laboratory testing technology. Users are invited to consult the appropriate
documentation for such information.
Alerts for Danger, Warning, and Caution
DANGER
DANGER indicates an imminently hazardous situation which, if not avoided, will
result in death or serious injury.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, could
result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe
practices.

B49006AH
x
Safety Notices
Safety Precautions
Safety Precautions
WARNING
Risk of operator injury if:
• All doors, covers and panels are not closed and secured in place prior to and
during instrument operation.
• The integrity of safety interlocks and sensors is compromised.
• Instrument alarms and error messages are not acknowledged and acted upon.
• You contact moving parts.
• You mishandle broken parts.
• Doors, covers and panels are not opened, closed, removed and/or replaced
with care.
• Improper tools are used for troubleshooting.
To avoid injury:
• Keep doors, covers and panels closed and secured in place while the
instrument is in use.
• Take full advantage of the safety features of the instrument.
• Acknowledge and act upon instrument alarms and error messages.
• Keep away from moving parts.
• Report any broken parts to your Beckman Coulter Representative.
• Open/remove and close/replace doors, covers and panels with care.
• Use the proper tools when troubleshooting.
CAUTION
System integrity could be compromised and operational failures could occur if:
• This equipment is used in a manner other than specified. Operate the
instrument as instructed in the product manuals.
• You introduce software that is not authorized by Beckman Coulter into your
computer. Only operate your system’s software with software authorized by
Beckman Coulter.
• You install software that is not an original copyrighted version. Only use
software that is an original copyrighted version to prevent virus
contamination.
CAUTION
If you purchased this product from anyone other than Beckman Coulter or an
authorized Beckman Coulter distributor, and, it is not presently under a Beckman
Coulter service maintenance agreement, Beckman Coulter cannot guarantee that
the product is fitted with the most current mandatory engineering revisions or
that you will receive the most current information bulletins concerning the
product. If you purchased this product from a third party and would like further
information concerning this topic, contact us.

B49006AH xi
Safety Notices
Symbol Explanations
WARNING
California Proposition 65:
This product may contain chemicals known to the State of California to cause
cancer and birth defects or other reproductive harm.
Symbol Explanations
Symbol meanings
Caution / Warning
Electrical shock hazard. Operate with caution!
Possible laser radiation inside.
Biohazard
Potential pinch point.a
a. This label only appears on the Plate Loader Module.
Potential puncture hazard.a
CE = Conformité Européenne
A “CE” mark indicates that a product has been assessed before being placed on the market,
and has been found to meet European Union safety, health, and/or environmental
protection
requirements. It is a mandatory marking for various product categories that are placed into
service within the European Economic area.
Consider all materials (specimens, reagents, controls, and monoclonal antibodies) and
areas these materials come into contact with as being potentially infectious.
Wear appropriate barrier protection and follow safe laboratory procedures when handling
any material in the laboratory.

B49006AH
xii
Safety Notices
Symbol Explanations

xiii
Contents
Revision History,iii
Safety Notices,ix
Alerts for Danger, Warning, and Caution,ix
Safety Precautions,x
Symbol Explanations,xi
Introduction,xxvii
Overview,xxvii
How to Use Your Manual,xxvii
About this Manual,xxvii
Conventions Used,xxix
Graphics, xxix
CHAPTER 1: System Overview,1-1
Overview,1-1
Product Description,1-1
Main Components,1-2
Optical Components,1-4
Wavelength Division Multiplexer (WDM),1-5
Optical Fiber,1-9
Fluidics System,1-10
Fluid Containers/Cubitainers,1-11
Fluidics Module,1-12
Sample Station,1-14
Sample Tube Holder Positions,1-15
Plate Loader Components,1-16
Plate Holder Components,1-18
System Configuration,1-20
System Configuration [CytoFLEX],1-20
System Configuration [CytoFLEX LX],1-22
Consumables and Supplies,1-24
Reagents,1-24
Material Safety Data Sheets (SDS/MSDS),1-24
Ordering Information,1-24

xiv
Contents
Instrument Specifications,1-25
Dimensions [CytoFLEX],1-25
Dimensions [CytoFLEX LX],1-25
Installation Category,1-25
Pollution Degree,1-25
Acoustic Noise Level,1-25
Electrical Ratings,1-25
Cytometer,1-26
Performance Characteristics,1-28
Performance Characteristics [CytoFLEX],1-28
Performance Characteristics [CytoFLEX LX],1-29
Reagent Limitations,1-29
CHAPTER 2: Using the CytExpert Software,2-1
Overview,2-1
Launching the Software,2-1
Main Software Screen,2-1
Start Page,2-2
Acquisition Screen,2-3
Acquisition Screen Navigation,2-4
Collection,2-5
Collection [With Plate Loader],2-6
Test Tubes,2-7
Plot area,2-8
Status Bar,2-9
Analysis Screen,2-9
Compensation Experiment Screen,2-10
QC Experiment Screen,2-12
QC Report Screen,2-12
QC Experiment Screen,2-13
QC Screen Navigation,2-13
Software Menu,2-14
Acquisition and Analysis Screen Menu,2-15
User Management,2-22
Creating, Deleting, and Modifying Users in User Manager,2-24
Creating a New User in User Manager,2-24
Deleting Users in User Manager,2-25
Modifying Users in User Manager,2-25
Unlocking a User Account,2-26
Resetting a User Passwords,2-26
Changing a User Password,2-26
Role Management,2-26
Creating, Deleting, and Modifying User Roles in Role Manager,2-28
Creating New User Roles in Role Manager,2-28
Deleting User Roles in Role Manager,2-29
Modifying User Roles in the Role Window,2-29

xv
Contents
Account Policies,2-30
User Management Operation Log,2-31
Viewing and Exporting User Logs,2-31
Graphic and Gating Styles,2-32
Plots,2-32
Gates,2-33
Software Settings,2-34
Language Settings,2-37
Setting Up CytExpert Application Programming Interface (API) Test
Client,2-37
CHAPTER 3: Daily Startup,3-1
Overview,3-1
Pre-Startup Inspection,3-1
Check Waste and Reagent Levels [4 L Fluid Containers],3-2
Check Waste and Reagent Levels [10 L Fluid Cubitainers],3-4
Power Source Inspection,3-5
Workstation Connections Inspection,3-5
Turning On the Instrument,3-6
Logging Into the Software,3-6
Logging Out of the Software,3-9
Selecting the Proper Sample Injection Mode,3-9
Using Semi-Automatic Injection Mode,3-10
Using the Manual Injection Mode,3-11
Selecting the Plate Loader Sample Injection Mode [With Plate
Loader],3-12
Using Plate Loader Injection Mode,3-12
Running the System Startup Program with the Single Tube
Loader,3-14
Running the System Startup Program [With Plate Loader],3-17
Selecting Experiments from the Start Page,3-24
Initializing the Instrument,3-25
CHAPTER 4: Instrument Quality Control and Standardization,4-1
Overview,4-1
Preparing the QC Sample,4-3
Required Materials,4-3
Preparation Process CytoFLEX Daily QC Fluorospheres,4-3
Preparation Process CytoFLEX IR Daily QC Fluorospheres,4-3
Preparing the QC Sample [With Plate Loader],4-4
Required Materials,4-4
Preparation Process CytoFLEX Daily QC Fluorospheres,4-4
Preparation Process CytoFLEX IR Daily QC Fluorospheres,4-5
Importing Lot-Specific Target Values,4-6

xvi
Contents
Collecting QC Data,4-10
Collecting QC Data [With Plate Loader],4-12
Confirming Results,4-16
Creating Levey-Jennings Charts,4-19
QC Result Manager,4-21
Standardization,4-22
Adding, Editing, Importing, and Deleting Standardization Laser
Target Values in the QC Experiment,4-23
Adding a New Standardization Item,4-24
Editing Standardization Item Parameters,4-25
Importing a Standardization Item,4-26
Deleting Standardization Items,4-26
Applying the Standardization in QC,4-26
CHAPTER 5: Data Acquisition and Sample Analysis,5-1
Overview,5-1
Creating an Experiment,5-1
Creating an Experiment [With Plate Loader],5-3
Setting Sample Wells,5-7
Modifying Well Settings,5-11
Applying Existing Well Settings to Additional Wells,5-12
Copying, Cutting, and Pasting Wells,5-12
Moving the Location of a Well,5-13
Running Samples,5-13
Creating a Heat Map,5-15
Refreshing a Heat Map,5-21
Modifying Existing Heat Map Settings,5-22
Deleting an Existing Heat Map,5-23
Exporting a Heat Map,5-23
Load Sample and Record Data,5-24
Before Running Samples,5-24
Verifying, Selecting, Editing, and Creating Detector
Configuration,5-25
Setting Up Violet Side Scatter (VSSC) Channel,5-29
Sampling and Collecting Data,5-32
Configuring Acquisition Settings,5-36
Changing the Tube Name,5-36
Setting the Channel and Label,5-36
Creating Plots and Gates,5-39
Creating and Adjusting Auto Gates,5-48
Turning Auto Recalculate On/Off,5-50
Adjusting Autogate Movement and Extent,5-51
Laser Settings,5-53
Setting Laser Target Power Settings [CytoFLEX LX Only],5-54
Adjusting the Gain,5-55
Adjusting the Threshold,5-57

xvii
Contents
Setting Collection Conditions,5-58
Setting Plot Display Conditions,5-60
Setting Customized Parameters,5-61
Setting Custom Statistics,5-62
Analyzing and Exporting Data,5-64
Exporting FCS Files,5-70
Exporting Single Tube Files,5-70
Exporting Multiple FCS Files,5-72
Exporting Plots or the Statistics Table of Multiple Tubes as Picture
Files,5-73
Importing and Exporting Instrument Settings,5-73
Importing Instrument Settings,5-74
Exporting Instrument Settings,5-74
Importing and Exporting Compensation Settings,5-75
Printing Graphics,5-75
Saving the Experiment,5-78
Concluding the Experiment,5-78
CHAPTER 6: Compensation,6-1
Overview,6-1
Creating a Compensation Experiment,6-2
Preparing the Compensation Sample,6-4
Using Control Samples to Generate the Compensation Matrix,6-4
Defining the Negative Population Using Unstained Samples,6-4
Running the Single Positive Control Samples,6-6
Calculating Compensation Values,6-8
Creating a Compensation Experiment [With Plate Loader],6-10
Preparing the Compensation Sample,6-13
Using Control Samples to Generate the Compensation Matrix,6-13
Calculating Compensation Values,6-13
Creating the Compensation Matrix from Previously Acquired Data,6-14
Adjusting Compensation,6-16
Manually Adjusting Compensation,6-16
Importing and Exporting Compensation,6-16
Importing Compensation Settings from Compensation Matrix
Files,6-16
Importing Compensation Settings from the Compensation
Library,6-17
Exporting Compensation Settings,6-19
Managing the Compensation Library,6-20
Adding Channels for Compensation,6-21
CHAPTER 7: Data Review,7-1
Overview,7-1
Copying Experiments and Importing Data,7-1

xviii
Contents
Copying a Previously Acquired Experiment,7-1
Importing Previously Acquired Data,7-1
Setting the Plots and Statistics,7-3
Opening the Analysis Screen,7-3
Creating Histogram and Dot Plot Overlays,7-5
Calculating Sample Volume and Concentration,7-7
Adjusting Compensation Settings,7-8
Exporting Results,7-9
CHAPTER 8: Daily Shutdown,8-1
Overview,8-1
Preparing the Cleaning Solution,8-1
Shutting Down the Instrument,8-1
Auto Shutdown [CytoFLEX LX Only],8-2
CHAPTER 9: Troubleshooting,9-1
Overview,9-1
Precautions/Hazards,9-1
Laser Related Hazards,9-1
Laser Beam Hazards,9-2
Laser Warning Labels,9-2
Hazard Labels and Locations,9-6
Biohazard Label and Location,9-6
Electrical Shock Hazard Label and Location,9-7
Caution Labels and Location,9-7
Plate Loader Hazard Labels and Location,9-8
Disposal Of Electrical Instrumentation,9-8
RoHS Notice,9-9
RoHS Caution Label,9-9
RoHS Environmental Label,9-9
Disposal Precaution,9-9
Troubleshooting Table,9-10
Backup and Restore,9-23
Backup,9-23
Restore,9-25
CHAPTER 10: Cleaning Procedures,10-1
Overview,10-1
Routine Cleaning, 10-1
Daily Clean,10-1
Daily Clean [With Plate Loader], 10-5

xix
Contents
Cleaning the Sample Station,10-7
Cleaning the Sample Probe,10-8
Deep Clean Procedure, 10-8
Cleaning the 4 L Sheath Fluid Container, 10-10
Cleaning the 4 L Waste Container,10-11
Nonscheduled Cleaning,10-13
Surface Cleaning and Disinfection, 10-13
Preparing the Instrument for Transport or Storage, 10-14
Lifting and Carrying Instructions,10-18
CHAPTER 11: Replacement/Adjustment Procedures,11-1
Overview,11-1
Routine Replacement/Adjustment,11-2
Front Cover Removal and Reinstallation,11-2
Removal, 11-2
Reinstallation,11-3
Right-Side Cover Removal and Reinstallation, 11-4
Removal, 11-4
Reinstallation,11-5
Filling the 4 L Sheath Fluid Container [CytoFLEX],11-6
Replacing the 10 L Sheath Fluid Cubitainer,11-7
Emptying the 4 L Waste Container [CytoFLEX],11-9
Emptying the 10 L Waste Cubitainer, 11-10
Managing the Maintenance Reminder, 11-14
Adding the Deep Clean Solution,11-17
Replacing the Sheath Fluid Filter,11-18
Replacing the Sample Probe and/or the Sample Peristaltic Pump
Tubing, 11-22
Replacing the Sample Probe Assembly [With Plate Loader], 11-28
Changing the Sample Probe from the Single Tube Sample Station to
the Plate Loader [CytoFLEX With Plate Loader],11-34
Changing the Sample Probe from the Plate Loader to the Single
Tube Sample Station [CytoFLEX With Plate Loader], 11-39
Inspecting the Liquid Flow Path for Leaks, 11-42
Priming the Flow Cell,11-43
Replacing the Plate Holder [With Plate Loader], 11-45
Plate Loader Module Removal and Reinstallation [With Plate
Loader],11-46
Removal, 11-46
Installation,11-50
Changing the Event Rate Setting, 11-55
Nonscheduled Replacement/Adjustment,11-56
Calibrating the Sample Flow Rate, 11-56
Calibrating the Sample Flow Rate [With Plate Loader],11-59
Setting Laser Delay,11-63
Replacing the Optical Filter,11-64
Replacing the Fuse,11-67
Replacing the Sheath Fluid Harness and/or Waste Harness, 11-69

xx
Contents
Changing Sample Mixing and Backflush Settings, 11-71
Calibrating the Plate Position [With Plate Loader],11-73
APPENDIX A: Instrument Installation,A-1
Overview,A-1
Instrument Transportation and Storage,A-1
Installation Environment Validation,A-2
Worktable,A-2
Ventilation and Cleaning,A-2
Power Source,A-3
Temperature and Humidity,A-4
Waste Disposal,A-4
Unpacking the Instrument and Inspecting the Materials for Defects or
Omissions [CytoFLEX],A-4
Installing the Instrument and Connecting the Equipment
[CytoFLEX],A-5
Installing the Instrument and Connecting the Equipment
[CytoFLEX LX],A-11
CytExpert Software Installation Options,A-12
Installing the Software [CytoFLEX],A-12
Required Materials,A-13
Installing the CytExpert Software,A-13
Installing the Instrument Configuration File,A-21
Starting the Software,A-24
Upgrading the CytExpert Software,A-24
Reinstalling the CytExpert Software,A-29
APPENDIX B: CytExpert Electronic Record Management,B-1
Overview,B-1
Software Menu,B-1
Experiment Management,B-2
Closed File System,B-2
Experiment Directory Management,B-3
Setting Up the Experiment Directory,B-3
Folder Hierarchy Management,B-4
Experiment Related Operations,B-5
Importing an Experiment/Template,B-6
Exporting an Experiment/Template,B-8
Log,B-11
Experiment Operation Log,B-11
System Operation Log,B-14
User Management Operation Log,B-15
Electronic Signature,B-16