BEHR S5 User manual

249101
behr S5 fully automatic steam distillation apparatus
Operating Manual

249101
Please read this operating manual carefully before starting use of your new behr S 5 steam distillation apparatus!
The manual introduces you to the use of the apparatus with clear and simple guidance.
For reasons of safety in use of the system, please follow all safety warnings carefully. These are designated in the
text by a warning symbol .
Additional information, which is useful and important for an understanding of the manner of operation of the
apparatus, is denoted by a stripe in the margin.
We wish you success in the use of the
behr
S 5 Steam Distillation Apparatus

249101
Safety Warnings
Danger of electric shock! Make sure that no liquids get into the apparatus housing or come in
contact with the electrical wires and connections. There are no components inside the appliance
which you need to operate. Repairs to electrical equipment must be carried out only by suitably
qualified and authorized personnel. Unplug the mains plug before opening the appliance.
Glass can break and cause injury! In working with glass components, observe all appropriate
safety precautions.
Risk of burns and injuries! When working with corrosive substances, follow the safety guidance
in the pertinent Safety Data Sheets.
Caution: reaction vessels get hot and can cause burns! During the distillation and for a short time
thereafter, do not touch any parts of the distillation system behind the Plexiglas door!
Do not operate the S5 steam distillation apparatus in damp or explosive atmospheres!
The maximum allowable humidity is 80%
The maximum allowable room temperature is 50 °C
Safety

249101
Contents
Scope of Delivery ...................................................................................................................................................... 5
Parts List 5
Use of the behr S 5in Chemical Analyses................................................................................................................ 5
Operating Conditions 5
Rear view of the behr S 5 .......................................................................................................................................... 7
Installing the behr S 5................................................................................................................................................ 8
Emplacing the steam distillation apparatus 8
Connecting the hoses and tubing 8
Electrical connections 9
Connecting to the electrical power line 9
Calibrating the pH electrode 9
Filling the Steam Generator 10
Front view of the behr S 5 ....................................................................................................................................... 12
Turning the steam distillation apparatus on ......................................................................................................... 13
Preparations 13
Switching the S 5 on 13
Distilling with the behr S 5 ...................................................................................................................................... 14
The operating control unit 14
Programming the S 5 14
Modifying a distillation program 15
Distilling 17
Before shutting the S 5 down ................................................................................................................................. 23
Conserving the pH electrode 23
Rinsing the tubes 23
Maintenance............................................................................................................................................................. 24
Checking the steam delivery rate 24
Checking the Reagents Pumps 25
Cleaning the NaOH Lines 25
Cleaning the Distillation Head 26
Changing the Viton Stopper................................................................................................................................... 27
Changing the Steam Tubing ................................................................................................................................... 28
Emptying the Steam Generator 30
Adapting the behr S 5 to your personal requirements......................................................................................... 31
Choosing the language 31
Configuring the level sensor 31
Setting the time 31
Selecting a titration unit 31
Cooling Water Source Setting 31
Setting the Date 31
What to do if ............................................................................................................................................................ 32
How to Display Current Operating Data 32
Error messages 32
Spare Parts............................................................................................................................................................... 36
Technical Specifications......................................................................................................................................... 36
Support .................................................................................................................................................................... 36
Overwiew: Menus and displays of the behr S 5.................................................................................................... 37

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© behr Labor-Technik 2013
249101
Scope of Delivery
Immediately on receipt, check the contents of the deli-
very for absence of damage and completeness.
A claim for damage in transport, which is evident on the
outside of the packing, must be immediately submitted
to the carrier (postal , rail or road haulage carrier) – see
the shipping label on the package.
If components are damaged, but no damage to the
external packing was evident (concealed transport
damage), contact the behr customer service immedia-
tely (also in the event of other complaints). The address
is:
behr Labor-Technik GmbH
Spangerstraße 8
D-40599 Düsseldorf
Téléphone : ([phone]
Télécopie : ([phone]
E-Mail: [e-mail]
Please check the contents against the following list.
Parts List
behr S5 distillation apparatus, completely assembled
Hose set:
1 Water inlet hose, 10/17, with ½“ and ¾“ standard fit-
tings, 2 m
Hoses marked with numbers:
, , : 3 x PVC tubing, 4/7, 2 m
, : 2 x PVC tubing, 8/12, 2 m
: Verprene tubing, 8/12, 2 m
(The cooling-water inlet hose, the cooling-water outlet
hose and the steam-generator feed hose come in
a separate hose package)
3 PVC tube, 6 x 1, 420 mm
1 PVC tube, 10 x 1, 420 mm
1 PP tube 10 x 2, 400 mm
1 Electrical (mains) cable
1 Crucible tongs
1 spare for steam hoses
1 spare Viton stopper
1 screwdriver 3 mm
1 screwdriver 2.5 mm
Use of the behr S5
in Chemical Analyses
The behr S 5 is an effective automatic distillation appa-
ratus for use in various applications. Principally, it finds
application in the steam distillation of Kjeldahl ammonia
solutions. In this application, the automatic addition of
NaOH, H2O and H3BO3, as well as the automatic drain-
ing of spent sample waste, provide for a high degree of
safety and convenience in use.
If you want to use the device for other kinds of anal-
yses, please ask the behr customer service if it is fit
for use with the chemicals needed for this purpose.
Be certain to carefully implement the following guidance
for the sake of safety in operation and the greatest pos-
sible working life of the behr S 5:
— Operate the apparatus always in accordance with
the instructions and warnings in this operating
manual!
Modification or changes to the apparatus are
unauthorized and lead to termination of the guar-
antee. Modifications may result in serious opera-
tional safety hazards and /or reduced reliability of
the S5.
— Do not expose the steam distillation unit to cor-
rosive vapors, such as acids, bases or organic
solvents!
Operating Conditions
Operate the behr S 5 steam distillation apparatus
under normal laboratory conditions.
The S 5 steam distillation apparatus may be con-
nected to a cold water tap having a standard ½“
pipe thread.
Scope of Delivery

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behr S 5
249101
overpressure - steam outlet
RS232-1 Titration Unit
Parallel-PrinterRS232-2 / Titr.Unit
USB
Level Detectors Power
1.1
1.5
1.2
1.3
1.4
Figure. 1: behr S 5 – rear view
See
Fig. 3
See
Fig. 2

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© behr Labor-Technik 2013
249101
RS232-1 Titration Unit
Parallel-PrinterRS232-2 / Titr.Unit
USB
Level Detectors Power
3.13.2
3.3
3.4
3.5
3.6 3.7
H BO3 3
1
Water outlet
4
Cooling water inlet
with filter
H O Sample2
2
Pump for suction
sample
5
NaOH
3
H O for steam
generator
2
6
Rear view of the behr S5
Fig. 1: Overview of the rear side
1.1 Air vent
1.2 Overpressure steam release
1.3 Steam generator overflow outlet
1.4 Steam generator drain
1.5 Excessive temperature circuit breaker
Fig. 2: Tubing connections
-- Cooling water inlet connection with filter
sieve; ¾“ connection for 10/17 PVC hose
1Boric acid; Connection nipple for 4/7 PVC tub-
ing
2sample dilution water; Connection nipple for 4/7
PVC tubing
3NaOH.
Connection nipple for 8/12 PVC-tubing
4Cooling water outlet, 8/12 PVC tubing
5sample waste disposal;-Connection nipple for
8/12 Verprene tubing
6H2O for steam generation; Connection nipple
for 4/7 PVC-tubing
Fig. 3: Electrical connections
3.1 Socket for electrical (Mains) power cable
3.2 Socket for level monitoring
3.3 Reserved for future use
3.4 RS 232 port for titrator
3.5 RS 232 port for software updates
3.6 25-pin port for titrator
3.7 Printer port
Rear view of the behr S 5
Figure 3: Electrical connectors
Figure 2: Hose connectors

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behr S 5
249101
Installing the behr S5
In installing the S 5, be certain to adhere to all buil-
ding codes pertaining to plumbing and to waste
water disposal regulations.
In selecting the location for installation, keep in mind
that the hoses and tubing provided have a length of
2 meters.
Place the behr S 5 steam distillation apparatus on a
sturdy horizontal flat laboratory bench top, with access
to both water tap and drain and sufficient space for the
reagent containers below the bench top.
Select a cold water tap to which you can permanently
attach the hose for the cooling water supply.
Faucets providing a mixture of hot and/or cold
water should not be used.
The water pressure must be at least 0.5 bar for
the proper functioning of the water pressure
safety monitor.
The steam distillation unit is completely assembled by
the manufacturer. Please unpack it carefully.




Emplacing the steam distillation
apparatus
Place the apparatus on the bench top.
Unpack the accessory parts.
First, put the reagent containers in place:
Container for distilled water
Container for sodium hydroxide (NaOH)
Container for boric acid (H3BO3)
Container for sample waste
The optimal location for these containers is directly
beneath the laboratory bench top.
In no case should the reagent containers be
placed at a level higher than the steam distilla-
tion apparatus.
Connecting the hoses and tubing
Pay careful attention to the labels of the hose and
tube connections found on the back of the S 5.
Insert the ends of the three 6 x 1 PVC tubes into the
three 4/7 PVC tubing segments numbered , ,
and .
Connect the free ends of the PVC tubing segments to
the following connection nipples on the rear of the S 5:
H2O for steam generator (),
H2O sample ().
Insert the PVC tubes into the container for distilled
water.
Insert the end of the 10 x 1 PVC tube numbered into
an 8/12 PVC tubing segment. Insert the 10x1 PVC tube
into the container for NaOH, and connect the free end
of the PVC tubing segment to the connection nipple
labelled NaOH () on the rear of the S 5.
Make certain that the tubes are securely inserted
in the PVC tubing segments.
Insert the third 6x1 PVC tube into the container for the
boric acid and connect the free end of the PVC tubing
segment to the connection nipple labelled H3BO3()
on the back of the S 5.
Using the pressure-resistant fabric-reinforced hose, con-
nect the cold water tap ½“ pipe thread to the ¾“ fitting
labelled cooling water inlet with filter on the rear of
the S 5.
Finally, connect the two discharge tubing segments:
Insert an end of the Verprene hose into the PP tube.
Connect the Verprene tubing segment to the connec-
tion nipple labelled pump for suction sample () and
insert the PP tube into the container for sample waste
discharge.








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© behr Labor-Technik 2013
249101
H BO3 3
1
Water outlet
4
Cooling water inlet
with filter
H O Sample2
2
Pump for suction
sample
5
NaOH
3
H O for steam
generator
2
6
Installing the behr S 5
Connect the 8/12 PVC tube segment to the connection
nipple labelled water outlet () and lead the other end
to a drain.
File the tubing of the titration device and the electrode
cable through the opening (4.9, cf. page 10).
Screw the tubing to the titration device. Connect the
other side to the tirator (see user’s manual of the tira-
tor).
Make sure the hoses are laid out without kinks.
Otherwise the suction pumps cannot deliver cor-
rectly. Analysis errors may occur as a result, or
distillation may be aborted due to lack of water
in the steam generator. Also make sure the waste
water from the discharge tubes can discharge
freely. This is most important for the distillate
discharge hose. If there are bends (siphons) in
the hose, discharging liquid might suck contents
from the distillation vessel, thus causing analyti-
cal errors.
Electrical connections
Connect the titrator connection cable to the appropriate
port of the S 5: Depending on the type of your titrator, it
will fit into the RS 232 socket (3.4) or the 25-pin socket
(3.6).
Screw the pH electrode to the electrode cable.
Connect the other sides of the electrode cable and of
the connection cable to the titrator.
Fill some KCl solution (KCl 3 mol/l) into the electrode
protection vessel.
Gently pull the protection cap off the electrode and put
the electrode into the electrode protection vessel.
When you are not doing analyses, always keep the
electrode in the protection vessel. Make sure there is
enough KCl solution in the vessel so as to keep the
electrode membrane wet. Do not leave the electrode
dry; it would dry out and become useless.
In case you are using the container set KAS 40:
Connect the individual sensor cables from the vari-
ous reagent containers to the central junction box.
Then connect the cable from the central junction box
to the socket labelled LEVEL DETECTORS on the
rear of the S 5.
Connecting to the electrical power line
First, insure that the voltage specification stated on the
model identification sticker of your S 5 corresponds to
the electrical line voltage.
Insure that the electrical power MAIN SWITCH on the
front of the S 5 is set to “0“.
Connect the electrical power cable to the socket on the
rear of the S 5 identified as MAINS.
Connect the mains plugs of the S 5 and of the titrator to
a grounded electrical power socket.
Calibrating the pH electrode
See user’s manual of your titrator.


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behr S 5
249101
~ 40 mg NaCl
H2O for
Steam
Generator
1.
2.
3.
I
0
Filling the Steam Generator
If you fill the steam generator with pure
distilled / demineralised water the level
sensor cannot work. The steam generator
would overfill. Add a little NaCl to the first
water filling, as described here.
The level sensor is a conductivity sensor; it cannot
work if the water has not a conductivity of about 100
µS/cm. This is achieved by addition of NaCl. During
distillation, the salt will remain in the steam genera-
tor; it won’t affect distillation. Only after long use, the
salt may wear out. Then the steam generator must
be refilled, adding a little NaCl to the first filling again.
In a 1-liter beaker, dissolve about 40 mg of NaCl in
some distilled water, and fill up to 1 liter with distilled
water.
Put the suction lance of the water-supply hose into
the beaker.
Switch the steam distiller on.
The device will fill the steam generator from the beaker.
It needs about 0.7 liters. The pump will not draw this
amount in one step, but will stop at 0.2 liters and display
an error message.
If the pump has drawn considerably less than 0.7 lit-
ers and now displays an error message:
Switch the device off and, after a few seconds, on
again.
Repeat these steps until the pump will not deliver any
more and no error message is displayed.
If the pump does not stop delivering at all, causing
the steam generator to flow over, the level sensor is
defective or has been connected incorrectly.
As soon as the steam generator is full, the pump will
stop delivering and the steam generator will start heat-
ing.
Leave the suction lance in the beaker still; this way you
can check if the device is working properly.
Fill the beaker with distilled water.
Insert the titration vessel.
Perform some distillations and make sure the device
is working properly.









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© behr Labor-Technik 2013
249101
4.1
4.4
4.2
4.3
4.5
4.6
4.10
4.11
4.13
4.14
4.15
4.16
4.21
4.22
4.23
4.24
4.25
4.27
4.28
4.29
4.30 4.31 4.32
4.12
4.7
4.8
4.18 4.174.19
4.20
4.26
4.9
Figure 4: behr S5 – Front view

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behr S 5
249101
Front view of the behr S5
Figure 3: Diagram legend
4.1 Operating control unit: Chemical-resistant
plastic foil
4.17 Distillate level sensors (the 2nd is hidden
behind the stirrer)
4.2 Liquid crystal display (LCD) 4.18 Protection vessel for pH electrode
4.3 Control knob 4.19 Drip tray
4.4 Cooling water outlet 4.20 PTFE steam delivery tube
4.5 Screw-on connectors GL 14 4.21 Notch for positioning the steam delivery tube
between distillations
4.6 Cooling water inlet 4.22 Quick release fastener for holding the sample
vessels during distillation
4.7 Distillate suction tube, silicone 4.23 Plexiglass protective door (not shown)
4.8 Silicone delivery tube for H3BO34.24 Fastening screws for glassware mounting
4.9 Opening for electrode cable and titration tube 4.25 Glassware mounting
4.10 Distillate delivery tube, silicone 8/12 4.26 Viton stopper for sealing vessel mouth
4.11 Air release valve 4.27 GL 18 screw-on connectors with silicone gaskets
4.12 Titration device 4.28 PTFE delivery tube for NaOH
4.13 Stirrer 4.29 Distillation head, glass
4.14 Titration vessel 4.30 Polypropylene steam/water inlet junction with
polypropylene nut
4.15 Electrical power switch 4.31 GL 32 screw-on connector with silicone gasket
4.16 Lid opening for pH electrode 4.32 Condenser
Front view

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© behr Labor-Technik 2013
249101
Turning the steam distillation
apparatus on
Preparations
Acids and bases can cause burns and
other injuries!
In working with acids and bases, observe
the safety precautions prescribed for these
substances in the Material Safety Data
Sheets!
Fill the reagent containers with the appropriate chemi-
cals.
Verify by checking the tubing connections which rea-
gents are to be filled into which containers. Then fill the
containers accordingly:
Distilled or deionized water (e.g. from a behropur®
water deionizer)
NaOH, 32%
H3BO3, 2 - 4%
If it has not yet been done, empty the waste-water
container, disposing of the contents in accordance to
regulations.
Provide the cooling water supply
Open the water tap.
Switching the S5 on
Turn the power on by switching the MAIN SWITCH
(3.15) on the front of the S 5 to the position „I“.
The electrical power switch of the S 5 fulfills two func-
tions. It serves to turn the apparatus on and off and
also has an electrical circuit-breaker built in.
This circuit breaker operates on a similar principal to those used in homes. If too much current is drawn, it
shuts off.
The operation of this circuit breaker requires that a spring within it be cocked. For this reason, a some-
what greater pressure is required on the switch to
turn it on than is required in a similar switch without
circuit breaker.
Switch your titration unit and - if installed - your printer
on.



After turning the apparatus on, the display on the front
will briefly show an initialization notification. Immediately
thereafter, the pump begins filling the steam generator
and you see the following notification on the display:
steam generator filling
An hourglass symbolizes the ongoing process.
Next, the following notification appears:
steam generator heating
Again, the hour glass is seen on the display.
These two procedures can take a bit of time, especially
when the apparatus is switched on for the first time.
These procedures have been completed when the dis-
play switches to the „Start“ mode
START
OPTIONS
PROGRAM 01
The S 5 steam distillation apparatus is now ready for
operation.

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behr S 5
249101
behr
S5
Press button for
enter / stop
–+
Figure 3: Operating control unit
Distilling with the behr S5
The operating control unit
Programming the S5
After filling and heating of the steam generator you will
see the following display:
START
OPTIONS
PROGRAM 01
This is the main menu. From here you come to all other
menus and displays.
The option START is pre-programmed as a default set-
ting.
Distilling
Your behr S 5 steam distillation apparatus is pro-
grammed and operated easily with a single knob.
The principle remains in all cases the same:
Turning the knob enables you to choose an option. The
currently addressable option is recognized by being
highlighted with green text on a black background.
The knob may be turned in both directions. In doing this,
you will pass all possible options in the display shown
and will always again find the desired option on contin-
ued turning. Try this for yourself.
The desired option is implemented by pressing on the
knob.
The complete procedure of choosing by turning and
implementing by pressing will henceforth simply be
called “selecting”.
The following pages will show you how to program and
operate you steam distillation apparatus.

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© behr Labor-Technik 2013
249101
Modifying a distillation program
With the S 5, you can define 99 different distillation
programs. This allows you to define the appropriate
conditions and the appropriate quantities of reagents for
different analyses and different sample concentration
ranges.
The 100th programm, program no. 00, is a test program
to check the steam delivery rate of the device.
When defining a program, make a note of its purpose
and the settings you made.
START
OPTIONS
PROGRAM 01
Turn the operating control knob clockwise or counter-
clockwise until the menu item Program is highlighted.
Then press the knob once. You will now see the follow-
ing display:
Prog.Number
01
<<== ==>>
You can now select which of the 99 programs of the S 5
you wish to enter or modify. Simply turn the operating
control knob until Prog.Number appears in green on a
black background. If you now press the knob once, you
will be able to dial to another number. Turning the knob
clockwise advances the display to the higher numbers,
while turning counterclockwise brings you to the lower
numbers. At both ends of the number range, continued
turning of the knob will bring you to the other end of the
range, specifically
turning clockwise beyond 99 will bring you
back to 00
turning counterclockwise from 00 will bring you
to 99.
By pressing on the operating control knob you will then
enter the programming options of the selected program
number.
If you are working with the S 5 for the first time, then
leave the program number selection on 01.
Pump delivery rates depend on the position of the
chemical vessels (desk or floor) and the length of
hose lines.
By turning the operating control knob and pressing,
select the option ==>>.
You will now see the following menu displayed:
NaOH 3.0
Dist.Time 060
Dil. H2O 3.0
==>><<== back
Prog.Number 01
Read the menu items as follows:
Prog.Number 01 You can modify the settings of pro-
gram no. 01
DIL.H2ODelivery time for dilution water in
seconds. Possible entry values
range from 0.0 sec to 99.9 sec.
The factory default setting is
3.0 sec.
Delivery of the pump approx.
16 ml/sec.
80 ml/5 sec
965 ml/min
NaOH Delivery time for sodium hydro-
xide in seconds. Possible entry
values range from 0.0 sec to 99.9
sec. The factory default setting is
3.0 sec.
Delivery of the pump approx.
10 ml/sec.
51 ml/5 sec
610 ml/min
Dist.Time Distillation time in seconds. Possible
entry values range from 0 sec to 999
sec. The factory default setting is
300 sec.
<<== back
==>> further
If you wish to change an entry, simply turn the operat-
ing control knob to highlight the value of interest and
press the knob to access the entry value. Then dial the
desired value by turning the operating control knob and
enter it in memory storage by pressing the knob again.
Once all entry values have been entered according to
your wishes, select ==>> and press the operating con-
trol knob to bring up the following program menu.
Power 80%
DrainSample 010
Reac.time 010
Prog.Number 01
==>><<== back

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behr S 5
249101
Explanation:
Prog.Number 01 You can modify the settings of pro-
gram no. 01
REAC.TIME Delay time in seconds between
the end of NaOH addition and the
start of distillation. Possible entry
values range from 0 sec to 999
sec. The factory default setting
is 0 sec. For the distillation in the
Kjeldahl nitrogen determination,
do not change the preset default
of 000.
POWER Steam generator power. Possible
entry values range from 30% to
100%. The factory preset default
value is 80%. You can change the
electrical power for steam gene-
ration and thereby the intensity of
steam generation.
The steam generator is swit-
ched on and off on by a pressure
sensor. The on/off set points of
the pressure sensor cannot be
adjusted by the user. It is only
possible for the user to adjust the
steam generation power.
DRAINSAMPLE Run-time in seconds for the pump
draining the sample waste after
distillation. Possible entry values
range from 0 sec to 999 sec. The
factory default setting is 30 sec.
<<== back
==>> further
If you wish to change any of the values, select the
desired parameter by turning the operating control knob.
Press on the control knob to access the numerical entry
value. Then dial the desired value by turning the oper-
ating control knob and enter it in memory storage by
pressing the knob again.
Once all entry values have been entered according to
your wishes, select ==>> and press the operating con-
trol knob to bring up the following program menu:
DrainTitr 010
H3BO3 3.0
AutoEndPt Off
==>><<==
Prog.Number 01
Explanation:
AutoEndPt Automatic endpoint determination.
Before destillation, the S 5 deter-
mines the pH of the boric acid;
afterwards it titrates back to this
pH. If AutoEndPt is Off, titration
goes to a fixed endpoint. Not all
titrators allow automatic endpoint
determination!
DrainTitr Run-time in seconds for the pump
draining the distillate after titration.
Possible entry values range from
0 sec to 999 sec. The factory
default setting is 30 sec.
H3BO3 Delivery time for boric acid into the
titration vessel in seconds.
Possible entry values range from
0.0 sec to 99.9 sec. The factory
default setting is 3.0 sec.
Delivery of the pump approx.
14 ml/sec.
70 ml/5 sec
840 ml/min
<<== back
==>> further
If you wish to change any of the values, select the
desired parameter by turning the operating control knob.
Press on the control knob to access the numerical entry
value. Then dial the desired value by turning the oper-
ating control knob and enter it in memory storage by
pressing the knob again.
Once all entry values have been entered according to
your wishes, select ==>> and press the operating con-
trol knob to bring up the following program menu:
Meas.Time 020
Stirring 40%
SetPoint 4.6
<<==
Prog.Number 01
Explanation:
SetPoint Endpoint preset for the titra-
tion. Titration will go to this pH if
AutoEndPt is off.
Not all titrators allow endpoint
setting from the S 5. With some
titrators the endpoint must be set
on the titrator itself.
Meas.Time Time in seconds for the endpoint
determination if AutoEndPt is on
and the titrator allows automatic
endpoint determination. The fac-
tory default setting is 10 sec.
Distilling

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© behr Labor-Technik 2013
249101
Stirring Stirring power in % of maximum.
The factory default setting is 40%.
<== back
==> further
If you wish to change any of the values, select the
desired parameter by turning the operating control knob.
Press on the control knob to access the numerical entry
value. Then dial the desired value by turning the oper-
ating control knob and enter it in memory storage by
pressing the knob again.
You have now completed program no. 01.
Select <<==. By repeatedly pressing <<== you will
return to the main menu.
Distilling
Danger of electric shock! Make sure that
no liquids get into the cable connections or
the inside of the equipment.
Glass can break and cause injury! In work-
ing with glass components, observe all
appropriate safety precautions.
Risk of burns and injuries! When working
with acids and bases, follow the safety
guidance in the pertinent Safety Data
Sheets.
Caution: reaction vessels get hot and can
cause burns! During the distillation and for
a short time thereafter, do not touch any
parts of the distillation system behind the
Plexiglas door!
Do not operate the S 5 steam distillation ap-
paratus in damp or explosive atmospheres!
The maximum allowable humidity is 80%.
The maximum allowable room temperature
is 50 °C.
(Re)filling the reagent lines
Before you use your S 5 for the first time or after longer
periods of disuse, the delivery pumps and tubing should
be filled with the respective reagent, replacing any air or
distilled water in the lines. By this you make sure all rea-
gents will be delivered in the correct amounts.
— Inspect the reagent containers and the tubing
connections.
— Open the water tap.
Inserting the sample vessel
Guide the PTFE steam delivery tube (4.20) into the
mouth of an empty sample vessel. Seat the mouth of
the vessel properly against the Viton stopper above
(4.26) and secure the vessel in place from below by
seating it in the recess of the quick-release fastener
(4.22). Check for firm and complete seal of the vessel
mouth against the Viton stopper.
Take the pH electrode out of the protection vessel.
Rinse it with some distilled water over a beaker. Insert it
into the free opening in the titration vessel (4.16).
Switching the steam distillation apparatus on
Close the protective door and turn the apparatus on
using the MAIN SWITCH.
In the main menu, select Options.

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249101
Performing the (re)filling
The following menu appears:
Device
Manual entry
<<==
Select MANUAL ENTRY. The display will then appear
as follows:
NaOH
Steam
Dil. H2O
<<== ==>>
Select DIL.H2Oand hold the operating control knob
depressed until water flows into the sample vessel.
Select NaOH and hold the operating control knob
depressed until sodium hydroxide flows into the sample
vessel.
Now select ==>> to proceed to the next menu.
Select H3BO3and hold the operating control knob
depressed until boric acid flows into the titration vessel.
Select DrainSample and hold the operating control
knob depressed until the liquid in the sample vessel has
been drained.
Select DrainTitr and hold the operating control knob
depressed until the liquid in the titratiopn vessel has
been drained.
Press the quick-release fastener (4.22) down and
remove the sample vessel.
Push the PTFE steam delivery tube into its retaining
notch (4.21).
By repeatedly pressing <<== you will return to the main
menu.
Test run
Especially reliable and reproducible results will be
obtained if you perform a „warm-up“ distillation without
sample before starting your daily analyses.
In the course of this first „warm-up“ distillation, the
steam will come in contact with cold glass compo-
nents, leading to a higher degree of condensate
formation. This could lead to excessive dilution of an
actual sample and to an excessive volume of liquid in
the sample vessel.
The introduction of steam at a temperture of approx. 106°C results in noises, which can be loud. These
noises are no cause for concern. With utilization of
our high-quality behrotest®sample digestion vessels,
models SR 3i, KJ 500 or KJ 750, the risk of glass
breakage is minimal.
Open the water tap.
Inserting the glass vessel
Glass can break and cause injury! In work-
ing with glass components, observe all
appropriate safety precautions.
Risk of burns and injuries! When working
with acids and bases, follow the safety
guidance in the pertinent Safety Data
Sheets.
Insert the steam delivery tube into an empty sample
vessel. Press the quick release fastener (4.22) down
and insert the sample vessel.
Check to insure that the sample vessel is firmly seated
against the Viton stopper (4.26).
Switching the steam distillation apparatus on
Close the protective door and turn the apparatus on
using the MAIN SWITCH.
Following the initialization, the main menu appears:
START
OPTIONS
PROGRAM 01
The menu selection Start is highlighted.
Starting the test run
Caution! Sample vessel is hot! During the
distillation and for a short time thereafter,
do not touch any parts of the distillation
system behind the plexiglass door.
Select Options in the main menu.
Distilling

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249101
The following menu appears:
Device
Manual entry
<<==
Select MANUAL ENTRY. The display will then appear
as follows:
NaOH
Steam
Dil. H2O
==>><<==
Select Steam. In this function the operating control knob
acts as an on/off switch.
The introduction of steam is started by a short press
of the operating control knob. Steam will continue to
flow into the sample vessel until the operating control
knob is pressed again.
Press the operating control knob briefly to start the
steam delivery. Continue the introduction of steam until
approximately 1 cm of condensate has accumulated in
the titration vessel.
Ending the test run
Press the operating control knob to end the distillation.
Now select DRAINSAMPLE. Hold the operating control
knob depressed until there is no more water in the sam-
ple vessel.
Select Drain Titr.Cell. Hold the operating control knob
depressed until there is no more water in the titration
cell.
Caution! Sample vessel is hot!
After distillation do not touch the sample
vessel by hand. Use the crucible tongs or a
suitable kind of protection.
Depress the quick-release fastener remove the sample
vessel.
Push the PTFE steam delivery tube into its retaining
notch.
By repeatedly pressing <<==, you will return to the main
menu.
Automatic Distillation with the behr S5
Glass can break and cause injury! In work-
ing with glass components, observe all
appropriate safety precautions.
Risk of burns and injuries! When working
with acids and bases, follow the safety
guidance in the pertinent Safety Data
Sheets.
Open the water tap.
Inserting the sample vessel
Insert the steam delivery tube (4.20) into the sample
vessel containing the digested sample material. Press
the quick release fastener (4.22) down and insert the
sample vessel.
Check for firm and complete seal of the vessel mouth
against the Viton stopper (4.26).
Switching the steam distillation apparatus on
Close the protective door and turn the apparatus on
using the MAIN SWITCH.
Following the initialization, the main menu appears:
START
OPTIONS
PROGRAM 01
The menu selection Start is highlighted.
Switching the titrator on
Make sure the titrator and - if installed - the printer is
switched on.
Insert the pH electrode in the lid opening (4.16).
Enter the sample data (e. g. sample weight) on your
titrator. Depending on the type of your titrator, you may
be able to enter the sample data beforehand, or have
them transmitted from a balance. See user‘s manual of
your titrator.

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249101
Starting the automatic distillation
Caution! Sample vessel is hot! During the
distillation and for a short time thereafter,
do not touch any parts of the distillation
system behind the plexiglass door.
Press the operating control knob briefly to start the dis-
tillation.
The display shows the status of the program execution
and the remaining time.
Interrupting or terminating the
distillation program
You can interrupt or terminate the distillation program
at any time during the course of program execution by
pressing the operating control knob.
Distillation will be interrupted, and this menu will appear:
ManualEntry
Function
terminate
<<== back ==>>
Now the device waits for your decision. You can abol-
ish this distillation; but you can also try to correct some
mistake and then go on distilling. In order to do the cor-
rections, you can even access the ManualEntry menu,
perform some manual entries and then return to this
menu.
Select
Manual
Entry
in order to go to the ManualEntry
menu, e. g. to deliver an additional
amount of a reagent. When you have
finished there, return to this menu by
selecting <<== until you are back here.
==>> In order to permanently terminate the
program. Your will be returned to the
main menu.
<<== In order to continue the program.
Ending the automatic distillation
Caution! Sample vessel is hot!
After distillation do not touch the sample
vessel by hand. Use the crucible tongs or a
suitable kind of protection.
After completion of the distillation program, depress the
quick-release fastener and remove the sample vessel.
Push the PTFE steam delivery tube into its retaining
notch.
— Sodium hydroxide and - to a lesser extent – boric
acid attack the pumps and tubing. It is therefore
advisable, following the last distillation every day
to rinse the delivery lines (pumps and tubing).
The pH electrode must not be left standing dry;
it would dry out and become useless. The proce-
dures you should follow are described on
page 22 in the section „Before shutting the S 5
down.“
Do not forget to refill the reagent lines and pumps
before using the apparatus again.
Distilling