BestScope BS-2081F User manual

BS-2081F BS-2083F
This manual is for users of BS-2081F,2083F Scientific Fluorescent Microscope in laboratory. To
ensure your safety, obtain optimum performance, and to familiarize yourself fully with the use of
this microscope, we recommended that you study the instruction manual carefully.
BS-2081F,2083F Scientific Fluorescent
Microscope Instruction Manual

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Contents
Microscope Part ................................................................................................................................2
Notice For Use...................................................................................................................................2
1.Components Name ........................................................................................................................4
2.Structure Overview........................................................................................................................5
3.1 Open Power Source(fig.1) ...................................................................................................7
3.2 Adjust Lighting and Switch Light Source (fig 2)...................................................................7
3.4 Mount Specimen And Adjust Stage( ...................................................................................8
3.5 Adjust Interpupillary Distance( ...........................................................................................9
3.6 Three Light Path Switching(fig 7) ........................................................................................9
3.7 Focusing( .............................................................................................................................9
3.8 Handwheel For Adjusting The Tightness Of Focusing (fig.9).............................................10
3.9 The Limit of Focusing ........................................................................................................10
3.10 Adjust The Pendulum Type Condenser...........................................................................10
3-11 Using Frosted Sheet(figure 13) .......................................................................................12
3-12 The Using Of Extensional Function Board (fig 14) ..........................................................12
3-13 “ECO” Function Key(fig 15) .............................................................................................12
3-14 Replace The Fuse ............................................................................................................13
3-15 “ECO” Device(fig 18) .......................................................................................................13
4.Installation ...................................................................................................................................14
4.1 Installation Diagram..........................................................................................................14
4-2-1 Install the Mechanical Slide Bracket (fig 1, fig 2) ...................................................15
4-2-2 Install The Mechanical Object Stage (fig 3, fig 4)...................................................15
4-2-3 Install Nosepiece....................................................................................................16
4-2-4 Install Camera Lens And Photo tube/0.5X C-mount (fig 6)....................................16
4-2-5 Install Eyepiece(fig 7).............................................................................................17
4-2-6 Install Objectives(fig 8) ..........................................................................................17
4-2-7 Install the Electric Condenser (fig 9)......................................................................18
4-2-8 Replace the Camera Interface (optional) (fig 10,11)..............................................18
4-2-9 Install The LED Lamp Chamber(fig 12)...................................................................19
6.Trouble shooting guide.................................................................................................................22
6.1 Optical Part .......................................................................................................................22
6.2 Mechanical System ...........................................................................................................24
6.3 Electrical System ...............................................................................................................25
FL-900 Epi- fluorescent Attachment................................................................................................26

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Microscope Part
Notice For Use BS-2081F,2083F
I. Safety Precaution
Symbol
Description
During use, the power box of microscope become very hot, so should not
be touched with bare hands.
Before using, please read the user manual carefully. If can not use
inappropriately, it will cause an accident that harm operators or damage
instrument
Power switch is open.
Power switch is closed.
II. Safety Precaution
1. Be careful before opening box. Don’t attach fingerprint and Perspiration on the camera
lens. Prevent camera and some accessories from dropping and damaging.
2. Don’t place microscope in direct sunlight, high temperature, high humidity or dusty, and
vulnerable to strong vibration. Ensure that the objective table is flat ,level and strong enough.
3. When working, near the microscope lamp room will be a little fever. Please ensure that
there is enough heat dissipation space around the room light.
4. Place the microscope on the grounding, avoid lightning strike.
5. To ensure safety, before change halogen lamp or fuse ,please guarantee the main
power switch is “O”, cut off light source, and wait for the light and lamp room are cooling. Check
input voltage: the input voltage indicated on the back of microscope and the power supply
voltage is consistent, otherwise it will lead to serious damage to the microscope.
6. Please use the special wire of the company to provide.

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III. Maintenance and Storage
1. All camera lenses are proofread and adjusted. Please tear open outfit
2. Revolving nosepiece and coarse micro focusing mechanism, which structure are
precise. Please don’t remove easily.
3. Instruments shall be kept clean, always remove dust. Please care about that don’t
contaminate optic components.
4. Smears on the optic lens, such as fingerprint，oil and so on ,which are wiped by
cotton，lens paper or gauze dipping anhydrous alcohol，xylene and ether.( Either,
xylene and alcohol are highly flammable, which can not near open fire. Please use
these chemicals in a well ventilated room.)
5. Don’t use organic solvent to wipe any optic components of microscope. If
cleaning ,please use neutral detergent.
6. When using, if the microscope are wet by liquid, please close power source and wipe
dry right now.
7. Don’t open any component parts of the microscope. Because it will degrade the
performance of the microscope.
8. Place instrument in a shady and dry space. If do not use microscope , please cover
with dust cover. Before covering, please ensure that the light box has cooled.

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1.Components Name BS-2081F,2083F
Eyepiece
Viewing head
Nosepiece
Objective
Stage
Condenser
Mainframe

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2.Structure Overview BS-2081F,2083F
1.Filter switching knob
2.Coarse and fine focusing handwheel
3.Focusing limiting and locking
Handwheel
4.Condenser height adjustment wheel
5.Lighting switching button
6.Lighting adjustment handwheel
7.Condenser
8.”ECO”infrared sensor
9.Coaxial pinion for X stage movement
10.Coaxial pinion for Y stage movement
11.Condenser disk
12.Biopsy clip
13.Screw for fastening extensional board
14. Extensional board

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BS-2081F,2083F
1.pushing rod for switching light path
2.Condenser fastening screw
3.Condenser height adjustment
wheel
4.”ECO”button
5.Aperture size adjustment knob
6.Fine focusing handwheel
7.Coarse focusing handwheel
8.Focusing limiting and locking
handwheel
9.Screw for fastening lamp chamber
10.Socket of lamp chamber
11.Grounding bar
12.Plug of lamp chamber
13.Lamp chamber
14.Allen wrench

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3.Adjustment And Operation BS-2081F,2083F
Fig 1
Fig 2
Fig 3
3.1 Open Power Source(fig.1)
Plug power source, toggle the main switch in ‘－’state，
which on the back of microscope.
3.2 Adjust Lighting and Switch Light
Source (fig 2)
1. Rotating the handwheel according to the direction
indicated by an arrow on the figure 2, the brightness will
increase. If not, brightness will reduce.
2. As shown in figure 2, when the light switch
button is push-in, the light on the top is opening, as
picture . When the light switch button is
push-out, the light on the bottom is opening, as picture
.
3.. 3.3 Light Source Socket(fig 3)
As shown in figure 3, the light socket and
the plug all with red dots. Before plugging in the socket,
please adjusting the direction until their red dots are
aligned, then plugging the plug.
☆the silk-screen is corresponding with the switch
button of lights. As shown in the figure, is
the socket of above light source. is the
socket of under light source.
Red dot

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BS-2081F,2083F
Fig 4
Fig 5
Fig 6
★when switching light button, if found the button
is not matched with the shown in the figure,
please check that
3.4 Mount Specimen And Adjust
Stage(fig 4, fig 5)
Push the cover glass slowly into the jaw, then clamp
glass gently.
Rotate handwheel to move ruler athwartships or
length ways. The specimen will be moved to a place
you need.
★Be careful to change objectives. When you have
observed the specimen with a Low-power objective
and needed to change objective, objective may
encounter specimen.
★The degree of tightness of handwheel can be
adjusted. Please Draw down handwheel A and find
two adjusting ring(B,C),then rotate ring. B is the
adjusting ring of X direction. C is the adjusting ring
of Y direction.
★the altitude of handwheel also can be adjusted.
Draw down handwheel A and D can rise integral
handwheels. You can also adjust it to negative
direction and rise handwheels.

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Fig 7
Fig 8
Fig 9
3.5 Adjust Interpupillary Distance(fig 6)
The range of interpupillary distance is 47mm~78mm. when
observing with binocular, holding the prism table and
pivoting to adjust the interpupillary distance, until two sides
of field roll into one and observe with binocular comfortably.
3.6 Three Light Path Switching(fig 7)
Icon
Operation
Proportion of camera
brightness
Push rod in
completely
100:0
Push rod to
middle
20:80
Push rod out
completely
0:100
3.7 Focusing(fig8,fig 9)
1. When the camera is not used.
Completely push the light path switching rod (fig.7) and
observe by objective of 10X. In order to avoid the specimen
and objective collision, we should rise mechanical object
stage and let the specimen near the objective. Widdershins
rotating the coarse adjustment knob slowly and dropping the
specimens, then searching image with the objective of 10X.
At last, focusing to clear with fine-turning handwheel.
2. When the camera is used
Push the light path switching rod completely(fig.7) and
binocular observing, until the image is clear, then pulling out
the rod, observing the image through the video, which
connects with microscope.
Lable of switching
light path
Pushing rod
Handwheel for adjusting
tightness of focus

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Fig 10
Fig 11
Fig 12
On both sides of the fine focusing handwheels are
removable. According to the direction of arrow, hold one
end of handwheel and make another cock. Then stir it from
the cock end. About install it, only need to align the center
of handwheel and assemble it. This function is helpful to
exchange two fine adjusting handwheels and avoid cause
the collision between fine adjusting handwheel and stage.
3.8 Handwheel For Adjusting The
Tightness Of Focusing (fig.9)
Accord the direction indicated by an arrow in figure 8 to
adjust the tightness of handwheel. The more rotating, the
tighter of the focusing handwheel, otherwise looser.
3.9 The Limit of Focusing (fig 10)
In actual use, if you need to lock the focusing limit
position(the height of platform), you can rotate the
handwheel tightly at the focusing limit position according to
the direction indicated by an arrow on the figure 3.
3.10 Adjust The Pendulum Type
Condenser (fig 11, fig 12)
● Condenser center should be coaxial with optical axis of the
objectives, which has been adjusted well before leaving
factory, so users need not to adjust by self.
● The top of the condenser, which has been adjusted before
leaving factory, so users need not to adjust by self.
●Rotating the focusing handwheel of condenser①,
condenser will move up and down. when using high power
objective, condenser will up. When using low power
objective, condenser will move down.
Handwheel for limiting and
locking focusing

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Fig 13
Condenser centering:
1. Rotating the focusing handwheel of condenser① and
rising the condenser to the top position.
2. Focusing the specimen with the objective 10x.
3. Rotating the field diaphragm ②, shrinking image of field
diaphragm to view.
4. Rotating the focusing handwheel ① to focus the image
of field diaphragm.
5. Using spanner to rotate screw③ and move the field
diaphragm to view center.
6. Opening the field diaphragm step by step. If the image of
field diaphragm at center and in a field of view, the
condenser has been centered.
7. In an actual use, increase the field of diaphragm and the
image will exterior contact with view.
8. The adjustment of aperture diaphragm④
Aperture diaphragm is to adjust numerical aperture, not to
adjust brightness. When the aperture diaphragm is opened
to the 70%~80% of objective aperture, it will get enough
good image contrast.
9. The controlling of the field of aperture diaphragm
When operating, rotating the field diaphragm ② and
shrink the field of aperture diaphragm, then observe it. If
the image is blurry, you can rotate the focusing handwheel
of condenser. rising bracket of condenser, the image will be
clear, then rotating field diaphragm and adjusting the image
fulls of eyepiece, to reduce the noise of light and produce
the quality of image.
Knob of switching filter

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Fig 14
Fig 15
Fig 16
3-11 Using Frosted Sheet(figure 13)
As shown in figure 13, the state of using filter is push in
the rod. When push out the rod, filter is in an empty
state. LBD is a filter of increasing color temperature.
ND25 is a filter of 25% light transmittance. ND6 is a filter
of 6% light transmittance.
3-12 The Using Of Extensional
Function Board (fig 14)
As shown in fig13, converter reserves a slot to hold the
extensional function board. The ordinary light field
board has been inset the slot before The lens of
microscope leaving factory. If using other extensional
function board, only need to tear down and loosen
②, pull out the standard light field board, then inset the
extensional function board into the slot and freeze the
bolt of ② .
3-13 “ECO” Function Key(fig 15)
When people leave microscope after 15 minutes, the
light will be off and the indicator light will flash once
every 3 seconds. When people back, press ECO function
key, which will prompt light open.
Long press button for 3 seconds will cause red indicator
off and floodlight always lighting. If again Long press
button for 3 seconds, it will result in red indicator light
lighting and recover the first time working state.
ECO function
key

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Fig 17
Fig 18
3-14 Replace The Fuse (fig 16,
fig17)
As shown in the figure 16, first using the
general wrench push aside the drawer of fuse.
Then referencing figure 17, through the square
hole under of the fuse drawer gently drag the fuse
upward.
There is a thin glass in the middle of the fuse,
please operate carefully and gently when pushing
aside the drawer and drag the fuse.
★there is a thin glass in the middle of fuse, Before
pushing drawer and dragging fuse, please operate
gently.
3-15 “ECO” Device(fig 18)
As shown in the figure 17, the device can detect
the front object within 1meter.
When people observing within one meter ahead,
the device displays that red light has been bright,
and the light of microscope does not turn off.
When the light is not on, which shows the
microscope light source is in an off state

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4.Installation BS-2081F,2083F
4.1 Installation Diagram
The diagram as below shows the order of each component’s installation. The figures in the
diagrams show the installation steps.
★Before installing, please ensure that all components are clean. Don’t cut any component or
surface of glass.
★Keep the hex wrench provided well, which can be used When you changing the components.













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4-2Procedure BS-2081F,2083F
Fig 1
Fig 2
Fig 3
Fig 4
4-2-1 Install the Mechanical Slide
Bracket (fig 1, fig 2)
★Install the platform bracket according to the path
of ① in figure 1. The dovetail slot needs to
align with the slot of platform bracket, sliding it
until the screw fix it. Then use the hex wrench
tighten the screw shown as I in figure 2.
4-2-2 Install The Mechanical
Object Stage (fig 3, fig 4)
★Install the mechanical object stage according to
the path of number ② in figure 2. First align
the object stage’s hole center and the bracket
circle center, then object stage downward slides
the bracket locating ring
After placing the object stage steady, using the
hex wrench screw the bolt, which shown as I in
the figure 4, and the object stage will not waggle.
Ⅱ

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Fig 5
Fig 6
4-2-3 Install Nosepiece (fig 5)
Connect the plug of nosepiece with socket, then
inset the dovetail groove of microscope according
to the path ③ shown in figure 5. At last, use hex
wrench tight up the bolt shown as Ⅲ.
4-2-4 Install Camera Lens And
Photo tube/0.5X C-mount
(fig 6)
1. Install camera lens
Install camera lens into the microscope
groove according to the path ④ shown in the
figure 6, then use hex wrench tight up the bolt
shown as Ⅳand fix the camera lens.
2. Install photo acc(optional)
Accord the path of ⑤ shown in figure 6 to insert
the trinocular viewing unit into the microscope
head. Then use the hex wrench screw the fixed
bolt shown asⅤ.
★During install camera lens, please always pay
attention to hold camera lens, and prevent it from
falling and breaking.

Ⅲ

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Fig 7
Fig 8
★If not use the ternary camera lens and the photo
accessories, please use the corresponding dust cover
to cover the ternary attachment interface and
eyepiece interface, to prevent into ashes.
4-2-5 Install Eyepiece(fig 7)
1.Inset eyepiece into the eyepiece sleeve
according to the path ⑥ shown in the figure 7.
4-2-6 Install Objectives(fig 8)
1. Adjust the coarse focusing handwheel, until
object stage of the mechanical device stent down to
its lower limit.
2. Twist the minimum objective to a hole of the
nosepiece. Then move the nosepiece and clockwise
twist other objectives to the corresponding hole
according to the order of magnification from low to
high.
This installation method can ensure that during
using, it will be easy to change the magnification.
★Clean the objectives regularly. Because the
objective lens is sensitive to dust.

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Fig 9
Fig 10
Fig 11
★when operating, first under of the objective 10x
search the sample and focus, then switching to
other ratio of objective lens.
★When switching objectives, turn the objective
nosepiece. It does not ensure the objective lens
into the heart of the light path until hear the “click”
sound
4-2-7 Install the Electric
Condenser (fig 9)
Drop the small stent to the lowest location, and
Inset condenser into the small stent according to
the path ⑦ shown in the figure 9, until the Ⅷbolt
piles into the Ⅶslot completely. Then screwing in
the Ⅵslot to fasten the condenser according to
the shown direction and stick electric condenser
tightly.
4-2-8 Replace the Camera
Interface (optional) (fig 10,11)
As shown in the figure 10, spin out the Ⅹbolt a
distance in the direction shown by the arrow and
don’t tight the camera interface, then spin out it.
As shown in the figure 11, screw the camera
interface into the ternary camera barrel to an
appropriate position. Then screw in the number XI
bolt to fasten the camera interface.

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Fig 12
★In order to ensure the appropriate position of
camera interface, first use eyepiece to observe
and adjust the sample to a clean imaging position.
Then install the camera and observe. At the same
time, accordingly adjust the position of camera
interface until to the clearest position, then screw
the number Ⅺbolt.
★the way to ensure the appropriate position of
camera interface: first observe through eyepiece
and adjust specimen to a clear imaging position.
Then install camera and observe image. At the
same time, adjust camera interface until to a
appropriate position. Then screw bolt Ⅺtightly.
4-2-9 Install The LED Lamp
Chamber(fig 12)
Insert the lamp box into the position at back of
chassis base according to the path ⑨ shown in
the figure 12.
Then use wrench screw the Ⅻbolt, until the lamp
box is tight.