Leica AutoStainerXL User manual

Instructions for Use
Leica AutoStainerXL, V2.3 English – 10/2012
Order-No.: 14 0456 80101 RevC
Always keep this manual near the instrument!
Read carefully prior to operating the instrument!
Leica
AutoStainer XL
Automated slide stainer

3
Leica AutoStainer XL – Automated slide stainer
The information, numerical data, notes and value
judgments contained in this manual represent the
current state of scientific knowledge and state-
of-the-art technology as we understand it follow-
ing thorough investigation in this field.
We are under no obligation to update the present
manual according to the latest technical devel-
opments, nor to provide our customers with ad-
ditional copies, updates etc. of this manual.
For erroneous statements, drawings, technical
illustrations etc. contained in this manual we ex-
clude liability as far as permissible according to
the national legal system applicable in each indi-
vidual case. In particular, no liability whatsoever
is accepted for any financial loss or consequen-
tial damage caused by or related to compliance
with statements or other information in this
manual.
Statements, drawings, illustrations and other in-
formation as regards contents or technical de-
tails of the present manual are not to be consid-
ered as warranted characteristics of our
products.
These are determined only by the contract provi-
sions agreed between ourselves and our custom-
ers.
Leica reserves the right to change technical
specifications as well as manufacturing pro-
cesses without prior notice. Only in this way is it
possible to continuously improve the technology
and manufacturing techniques used in our prod-
ucts.
This document is protected under copyright laws.
Any copyrights of this document are retained by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – includ-
ing any electronic systems and media – requires
express prior permission in writing by Leica
Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Biosystems Nussloch GmbH
NOTE
Published by:
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: [phone]
Fax: [phone]
Internet: http://www.LeicaB iosystems.com

Instructions for Use V 2.3 – 10/2012
4
Table of contents
1. Important information .................................................................................................... 6
1.1 Symbols occurring in the text and ............................................................................................6
1.2 Designated use / improper use of the instrument .................................................................. 7
1.3 Qualification of personnel .......................................................................................................... 7
2. Safety................................................................................................................................. 8
2.1 Safety regulations ....................................................................................................................... 8
3. Instrument features ........................................................................................................ 9
3.1 Overview – instrument .............................................................................................................. 10
3.2 Standard delivery - packing list .............................................................................................. 12
3.3 Technical Data ........................................................................................................................... 13
4. Installation ..................................................................................................................... 14
4.1 Site requirements ...................................................................................................................... 14
4.2 Connection.................................................................................................................................. 14
4.2.1 Power .......................................................................................................................................... 14
4.2.2 Water supply .............................................................................................................................. 15
4.3 Battery backup - UPS (optional)..............................................................................................16
4.4 Remote alarm (optional) ........................................................................................................... 17
4.5 Fume control system ................................................................................................................. 17
4.6 Oven ............................................................................................................................................. 17
5. Operation ........................................................................................................................ 18
5.1 The control panel....................................................................................................................... 19
5.2 The main menu........................................................................................................................... 20
5.3 Menu map ................................................................................................................................... 21
5.4 Edit a program ............................................................................................................................ 22
5.4.1 Entering steps ............................................................................................................................ 23
5.4.2 Erasing steps .............................................................................................................................. 24
5.4.3 Inserting a blank step into a program .................................................................................... 24
5.4.4 Removing blank steps in a program ....................................................................................... 25
5.4.5 Saving a program ...................................................................................................................... 25
5.4.6 Deleting a program.................................................................................................................... 25
5.4.7 Copying a program .................................................................................................................... 26
5.4.8 Viewing a program .................................................................................................................... 27
5.4.9 Checking program compatibility .............................................................................................27
5.5 User-adjustable parameters .................................................................................................... 29

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Leica AutoStainer XL – Automated slide stainer
Table of contents
5.6 Oven ............................................................................................................................................. 29
5.7 Agitation (Dips) .......................................................................................................................... 30
5.8 Rack movement times ............................................................................................................... 31
5.9 Staining ....................................................................................................................................... 31
5.9.1 Reagent containers ................................................................................................................... 32
5.9.2 Wash system .............................................................................................................................. 32
5.9.3 Water saving .............................................................................................................................. 33
5.9.4 Loading slide racks ................................................................................................................... 33
5.9.5 Unloading racks from the exit drawer.................................................................................... 34
5.9.6 Unloading racks from other stations ...................................................................................... 34
5.9.7 Interrupting staining .................................................................................................................. 35
5.9.8 Aborting a rack .......................................................................................................................... 36
6. Cleaning and Maintenance ........................................................................................ 37
6.1 Cleaning the instrument ........................................................................................................... 37
6.1.1 Wash containers ....................................................................................................................... 37
6.1.2 Reagent containers ................................................................................................................... 37
6.1.3 Slide racks .................................................................................................................................. 37
6.1.4 Oven ............................................................................................................................................. 37
7. Trouble shooting ........................................................................................................... 38
7.1 Instrument failures .................................................................................................................... 39
7.2 Information and warnings ........................................................................................................ 40
7.2.1 During staining ........................................................................................................................... 40
7.2.2 During editing programs ........................................................................................................... 41
7.2.3 During SetUp .............................................................................................................................. 42
8. Warranty and service .................................................................................................. 43
APPENDIX 1 ................................................................................................................... 44
User-adjustable parameters ............................................................................................................... 44
APPENDIX 2 ................................................................................................................... 45
Consumables and accessories ........................................................................................................... 45
APPENDIX 3 ................................................................................................................... 46
Compatible staining programs ............................................................................................................ 46
Glossary .......................................................................................................................... 48

6Instructions for Use V 2.3 – 10/2012
1.1 Symbols occurring in the text and
Warnings and cautions appear in a
grey box and are marked by a
warning triangle .
Notes,
i. e. important information for the
user appear in a grey box and are
marked by an information sign .
Numbers in parentheses refer to
positions in the illustrations or to
the illustrations themselves.
Instrument type:
All information in this Instructions for Use applies
only to the instrument type indicated on the title
page.
A nameplate with the instrument serial number is
fixed on the back of the instrument.
Required information:
For all inquiries it is important to state the follow-
ing:
• instrument type
• serial number.
The Instructions for Use for the Leica
AutoStainer XL includes chapters dealing
with the following subjects:
Chapter 1 Manual structure including:
• Table of contents
• Important information
on this handbook.
Chapter 2 Safety
• Read this chapter before you
attempt to operate the
instrument!
Chapter 3 Instrument features
• General description
• Technical data
Chapter 4 Installation
• Site requirements
• Installation
Chapter 5 Operation
• Control elements
• Menu map
Chapter 6 Trouble shooting
Chapter 7 Cleaning and maintenance
Chapter 8 Warranty and service
Appendix 1 User-adjustable parameters
Appendix 2 Consumables
and accessories
Appendix 3 Compatible staining programs
Glossary
1. Important information
(5)
(Fig. 5)
their meaning

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Leica AutoStainer XL – Automated slide stainer
General
This Instructions for Use includes important information related to the oper-
ating safety and maintenance of the instrument.
The Instructions for Use is an important part of the product. It must be read
carefully and completely before setup and first use of the instrument and
must be kept near the instrument at all times.
If additional requirements on accident prevention and environmental pro-
tection exist in the country of operation, this Instructions for Use must be
supplemented by appropriate instructions to ensure compliance with such
requirements.
Be sure to read and comply with the safety instructions,
warnings and cautions in chapter 2, even if you are already
familiar with the operation and use of other Leica products.
1.2 Designated use / improper use of the instrument
- The AutoStainer XL has been designed for staining applications in medi-
cine, biology and industry.
- The instrument may be operated only according to the instructions con-
tained in this manual.
- Any other use is considered an improper use of the product.
1.3 Qualification of personnel
- The AutoStainer XL may be operated only by trained laboratory person-
nel.
- All laboratory personnel designated to operate the AutoStainer XL must
read this Instructions for Use carefully and must be familiar with all tech-
nical features of the instrument before attempting to operate the
AutoStainer XL.
1. Important information
To prevent damages to the instrument and specimens, only
those accessories and spare parts which have been authorized
by Leica may be installed or used with the instrument.

8Instructions for Use V 2.3 – 10/2012
2. Safety
2.1 Safety regulations
This instrument has been built and tested in accordance with the safety
regulations for electrical measuring, control, regulating and laboratory de-
vices.
In order to maintain this condition and ensure safe operation, the operator
must observe all the instructions and warnings contained in this Instructions
for Use.
For current information about applicable standards, please refer to the CE
declaration of conformity on our Internet site:
www.LeicaBiosystems.com

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Leica AutoStainer XL – Automated slide stainer
3. Instrument features
The AutoStainer XL is the result of an extensive research program to pro-
vide an innovative stainer which meets the quality requirements of the mod-
ern laboratory, as well as:
• high throughput,
• flexibility,
• safety.
The AutoStainer XL achieves its high throughput by means of an innovative
slide rack transfer mechanism which allows continuous loading of up 11
racks of 30 slides each.
The flexibility of AutoStainer XL also permits simultaneous processing of
slide racks according to different staining protocols so that Papanicolau
and Hematoxylin/Eosin staining can be conveniently performed at the same
time, without reprogramming or reagent changes.
The AutoStainer XL incorporates all of the features which ensure high flex-
ibility, convenience and above all, quality staining. A fan-forced oven is avail-
able to quickly dry slides and optimized wash stations result in rapid re-
moval of excess reagent. The minimum-carryover design of the slide racks
ensures that there are no drips and reagent life is extended.
The AutoStainer XL is safe to use and has an integral fume control system.
Rack loading and unloading is achieved by a unique two-drawer system
which means virtually no exposure to fumes.
The outstanding flexibility, throughput and quality-staining capability of
AutoStainer XL has set a new standard in staining excellence.

Instructions for Use V 2.3 – 10/2012
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3. Instrument features
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3.1 Overview – instrument