Bioneer ExiPrep 16 Plus User manual

User Manual (Ver. 1.1)
ExiPrep™16 Plus
Fully Automated Nucleic Acid Extraction System

Copyright © 2010 by Bioneer Corporation. All rights reserved.
Contents
I. Getting Started .............................................................................................................................................. 1
II. Safety Warnings and Precautions .............................................................................................................. 2
III. System Components and Specifications .................................................................................................. 5
1. System Components ............................................................................................................................................................... 5
2. Specifications ............................................................................................................................................................................. 6
3. System Views ............................................................................................................................................................................. 6
IV. Installing the
ExiPrep ™16 Plus .................................................................................................................. 7
V. DNA/ RNA Extraction ................................................................................................................................... 9
1. Contamination shield installation ...................................................................................................................................... 9
2. Sample Preparation .............................................................................................................................................................. 10
3. RUN ............................................................................................................................................................................................. 12
VI.
ExiPrep ™16 Plus Setup ............................................................................................................................. 15
1. Main Menu .............................................................................................................................................................................. 15
2. Registering a New User ...................................................................................................................................................... 19
3. Viewing Run History ............................................................................................................................................................ 20
4. Managing the Login Mode ............................................................................................................................................... 21
5. Managing the Automatic UV-Sterilization Mode .................................................................................................... 22
6. Controlling System Configuration .................................................................................................................................. 23
7. Administrator Management .............................................................................................................................................. 24
VII. Updating the
ExiPrep
TM16 Plus ............................................................................................................... 26
VIII. DNA/ RNA Extraction Program Number List ....................................................................................... 29
IX. Troubleshooting ......................................................................................................................................... 33
X. Warranty ...................................................................................................................................................... 37

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Fully Automated Nucleic Acid Extraction System
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I. Getting Started
Thank you for purchasing this Bioneer product.
We will try our best to provide satisfactory results to our customers.
This manual contains practical guidelines and cautions to be taken regarding the instrument.
Please read this manual carefully and thoroughly before using the instrument.
Website
▪Please visit us online at http://www.bioneer.com to obtain more information about ExiPrepTM 16
Plus. You can download up-to-date product information and new protocols.
General information
▪ ExiPrep™16 Plus is a trademark of Bioneer Corporation.
▪ The information contained in this manual is under copyright protection. It is unlawful to
reproduce part or all of the contents of this manual without the expressed written consent of
Bioneer Corporation.
▪ Bioneer Corporation reserves the right to alter, modify and otherwise make changes to the
instrument and manuals without prior notice.
▪ You must be used carefully UV Lamp. Detail can be found in Safety warning and Precautions.
Reversion
Cat. NumberA-5030 Rev. 1.1
April/2011

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Fully Automated Nucleic Acid Extraction System
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II. Safety Warnings and Precautions
The warnings and precautions stated below are for the correct and safe operation of the instrument. Please
heed all information for your safety. Bioneer Corporation does not assume responsibility for non-compliance
with the safety warnings and precautions stated below.
1. User and experimental precautions
1) Make sure that the power supply (100-240V, ~50/60Hz) is correctly connected to the power adapter
and that the power adapter is correctly connected to the instrument. Incorrect connection of the
power adapter and power supply can result in instrument damage or failure to turn on.
2) This instrument is intended for nucleic acid extraction. Please use as such.
3) The instrument may stop if the LCD panel is touched while connected to a PC via LAN cable. If
operating the instrument via PC software, please allow for the instrument to finish its programmed
movement before operating the LCD panel.
4) Please install the instrument on a flat surface.
5) Do not operate the instrument with wet hands as this may result in shock or instrument malfunction.
Please touch the power adapter cord with dry hands.
6) If the instrument is stopped either from operator error including improper accessory insertion or
manually halting the instrument during normal operation, you must re-initialize the instrument before
pulling out the Base Plate. Pulling out the Base Plate without prior initialization can lead to
instrument damage from movement interferences including a raised Heating Block or other
accessories stopped in motion. If Buffer Cartridges are inserted into the Base Plate, please re-
initialize the instrument or pull out the Buffer Cartridges to make sure the Heating Block is not in the
way of normal Base Plate movement.
2. Precautions regarding the operation environment
1) If the power plug is loose, do not use the instrument. Plug overheating may result in shock or fire.
2) Do not operate multiple instruments out of a single wall outlet. The load may cause overheating and
may lead to fire.
3) When plugging or unplugging the power adapter cord from a wall outlet, make sure your hands are
completely dry. Wet or moist hands may cause electric shock.
4) Avoid placing objects in the front and rear of the instrument.
5) Avoid installing the instrument in a dusty environment. Excessive dust may cause malfunction or
damage to the instrument.
6) Avoid installation near heat sources. This can cause fire.
7) Avoid installation near sources of water or damp locations. This can cause electrical shock, fire or
instrument malfunction.

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Fully Automated Nucleic Acid Extraction System
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8) Do not install near sources of flammable or corrosive gas. If there is a gas leak, do not touch the
power plug but open a window to circulate fresh air. Sparks from the power plug can cause fire and
explosions.
9) Do not disassemble or modify the instrument in any way. This can result in fire, electrical shock or
malfunction, and also voids your warranty.
3. Precautions and warnings regarding instrument installation
1) This is a precision instrument. Do not install in a location exposed to direct sunlight.
2) Install the instrument on a flat, solid surface that is flat and does not move.
3) When installing the instrument, make sure at least 15 cm separate the instrument from the nearest
wall.
4) Take caution not to damage the cooling fan mesh (located on the front-bottom) while installing.
4. Precautions and warnings regarding instrument operation
1) Dust off the power plug and insert the plug so that the connection is firm and does not wiggle.
Incomplete electrical contacts may cause fire.
2) Operate the instrument in an ambient temperature range of 15℃~30℃. Excessive exposure to heat
may affect the instrument and yield inexact results.
3) Operate the instrument within the recommended humidity range (20~80%, no condensation). Humid
conditions may cause corrosion or malfunction.
4) Do not place any objects next to or behind the instrument. The instrument may malfunction.
5) This instrument contains precision machined parts. Do not drop or severely agitate the instrument.
This can break the instrument and compromise the safety of the product.
6) When not using the instrument for a long period of time, turn the instrument off and unplug from the
wall outlet. Overheating and fire may occur.
7) The instrument automatically turns off the UV lamp and its operation when the instrument’s door is
open. However, just in case it does NOT turns off when the door is open, please make sure the UV
light does not directly expose to your eyes and skin.
5. Precautions and warnings regarding product usage and maintenance
1) This product must only be used for nucleic acid extraction and automatic aliquot. Do not use the
instrument for any use other than explicitly stated in the User Manual.
2) Do not modify or delete instrument-related information installed within the instrument.
3) Operate the LCD touchscreen using a non-sharp object. Nails and other sharp objects may damage
the product.
4) The instrument UV lamp will only operate if the door is completely shut. Make sure the door sensor
is free of foreign materials or obstructions.

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Fully Automated Nucleic Acid Extraction System
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5) Do not use powerful detergents or solvents to clean the outside of the instrument as this may cause
discoloration. If such chemicals are spilled on the instrument, immediately clean with a soft cloth.
6) Do not keep the instrument in an environment with high humidity. Damage from storage in these
conditions is classified as water damage and is not covered by warranty. Also, damage arising from
this type of exposure may be irreparable.
7) Disassembly and/or modification of the instrument voids the warranty and may be refused service.
8) Do not unplug the power adapter from the instrument while the instrument is in use. This may cause
the instrument to break.
9) If a burning smell is detected or the instrument seems to be excessively hot during operation,
immediately stop using the instrument and call your service representative.
10) Do not drop or impact the instrument. This is a direct cause of instrument damage and may void the
warranty.
11) Always verify that the Heating Block is in normal position before pulling out the Base Plate. If the
Base Plate is pulled out while the Heating Block is not in its normal, initialized position, the
interference in movement can cause Heating Block and other internal component damage and lead
to instrument malfunction. Since installed Buffer Cartridges obscure the view, re-initialize the
instrument or take out the Buffer Cartridges and visually inspect the position of the Heating Block
before pulling out the Base Plate.
12) The instrument automatically turns off the UV lamp and its operation when the instrument’s door is
open. However, just in case it does NOT turns off when the door is open, please make sure the UV
light does not directly expose to your eyes and skin.
13) When there is liquid in the Waste Tray in the equipment, take extra caution to push-in or pull-out the
base plate so the liquid does NOT overflow to the instrument inside. If the liquid overflows inside the
instrument, it may damage the instrument or cause the electrocution.
6. UV Lamp
1) UV lamp operation may create Ozone molecules. For the safety issue, the instrument is pre-
programmed the UV lamp operation for 5 minutes only. Please do NOT extensively use UV lamp
operations.
2) The Ultraviolet (UV) ray can seriously damage your eyes and skin when exposed directly (even
through indirectly). When you deal with UV lamp, make sure you are wearing proper protective
equipment.

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Fully Automated Nucleic Acid Extraction System
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III. System Components and Specifications
1. System Components
Part Name Cat. No. Qty. Check
ExiPrep™16 Plus A-5030 1 ea □
User’s manual 1 ea □
Waste tray 1 ea □
Elution tube rack 1 ea □
Disposable tip rack 1 ea □
Setup tray 1 ea □
Contamination Shield 1 ea □
Hole puncher (6 hole) 1 ea □
Adapter 1 ea
□
(Option) Multi Puncher -

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Fully Automated Nucleic Acid Extraction System
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2. Specifications
Dimensions (mm) 320 (W) x 487 (H) x 535 (D)
Weight 22 kg
Operating temperature 15 – 30°C
Operating humidity 20 - 80%, no condensation
Operating system Stand-alone
Electrical (Voltage / Frequency) 100-240 V, 50/60 Hz
Network support TCP/IP protocol
User interface 320 x 240 touch screen graphic LCD, 18 bit color
3. System Views

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Fully Automated Nucleic Acid Extraction System
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IV. Installing the ExiPrep™16 Plus
- Clean the area where the ExiPrep™16 Plus will be installed.
- Open the instrument door and remove the tie wrap from the belt.
- Make sure that all components are included. Refer to the list of components on page 5.
1. Open the door and remove the Screw-bolt (A) from the rail.
2. Remove the Plate (B) from the rail and the Syringe Block.
3. Remove the Screw-bolt (C) from the locking block which
immobilizes the belt.
4. Separate the Locking block (D, E) from the belt and remove
it.

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Fully Automated Nucleic Acid Extraction System
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IV. Installing the ExiPrep™16 Plus (continued)
5. Connect the power cable to the rear of the instrument.
6. Turn on the instrument. A power button will display on the
LCD touch screen to indicate normal power on.
7. Press the power button on the LCD screen to initialize the
instrument. A progress bar on the lower portion of the LCD
touchscreen will indicate initialization progress.
8. Open the door and remove the Sponge block (F) from the
Base Plate after initialization.
※If the instrument does not start even when power was supplied properly but initialization does not
complete in approximately 5 minutes, please contact Customer Service or your local Bioneer distributor
immediately.

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Fully Automated Nucleic Acid Extraction System
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V. DNA/ RNA Extraction
1. Contamination shield installation
1. From the ‘Menu’ screen, click ‘MISC SET’.
¾Pull out the Syringe block all the way front (outside)
Contamination shield.
CAUTION!
In order to use contamination shield, this must be installed on
the Base plate accessory.
2. Place the contamination shield on the lower-right side of the
Syringe block.
¾Contamination shield has a magnet which means if you place
on the upside-down right, it will stick to the holding bar.
3. From the ‘Menu’ screen, click ‘MISC SET’.
¾The base plate moves back to the initialization position
(inside the instrument).

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Fully Automated Nucleic Acid Extraction System
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V. DNA/ RNA Extraction (continued)
2. Sample Preparation
1. Set the Setup Tray (A) on a flat laboratory surface.
2. Insert the Elution Tube Rack (B), Disposable Tip Rack (C),
and Buffer Cartridge ①(D) into the Setup Tray.
¾The Elution Tube Rack and Disposable Tip Rack are
included with the instrument. The Buffer Cartridges,
Disposable Tips and Elution Tubes necessary for DNA/RNA
extraction are included in the DNA/RNA extraction kits
(sold separately).
3. Make sure you have purchased the correct kit for the sample
type and target nucleic acid type you desire. Insert the
desired number of Elution tubes (E) into the Elution Tube
Rack.
※Make sure the direction of the Elution Tube caps are laid out
as on the left when inserting into the Elution Tube Rack.
¾Each Elution tube is labeled individually from A1~H1, A2~H2
to A12~H12 for a total of 96.
¾For convenience, you may arrange your eluted nucleic acids
in the provided 96-well tube storage box
4. Insert the Disposable Tips (F) into the Disposable tip rack in
the same quantity and relative position as the Elution tubes.

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Fully Automated Nucleic Acid Extraction System
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V. DNA/ RNA Extraction (continued)
2. Sample Preparation (continued)
5. Punch holes in the sealing film of Buffer Cartridge ①
corresponding to the relative location and number of tubes and
tips, using the 6-Hole punch tool.
6. Punch holes in sealing the film of Buffer Cartridge ②using the
6-Hole punch tool in the same pattern as Buffer Cartridge ①.
7. Load samples into the ‘Sample Loading Well’ of Buffer
Cartridge ①.
8. Preparation for DNA/ RNA extraction is complete.
※Before punching holes through the sealing film, briefly shake Buffer Cartridges ①and ②to collect
reagents to the bottom of the wells and dissociate the silica magnetic beads that may have clumped
during storage. Clumping of the magnetic beads is natural and will not affect your results.

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Fully Automated Nucleic Acid Extraction System
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V. DNA/ RNA Extraction (continued)
3. RUN
1. Press the ‘START’ button to access the PREP SETUP screen.
2. Refer to the code list within this Manual or purchased Kit
Manual to select the three-digit code applicable to your
desired nucleic acid and sample source type.
3. Verify the ‘Prep Type’ and ‘Sample SRC’ of the three-digit
code you have entered.
4. Press the ‘Enter’ button to access the ‘elution volume’
selection menu.
5. Select the ‘elution volume’from the LCD touchscreen.
6. After selecting the desired ‘elution volume’press ‘ok’ to
complete PREP SETUP.
7. Open the instrument door and pull out the Base Plate.
8. Place all racks and Buffer Cartridges in their respective
locations on the Base Plate according to the CHECK LIST on
the LCD touchscreen.

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※Setup process according to the CHECK LIST
8a. Insert Buffer Cartridge ②on the Base plate.
¾Make sure the Buffer Cartridge fits snugly and exactly into its place.
Misplacement of the Buffer Cartridge may result in instrument break
down and malfunction.
8b. Insert Buffer Cartridge ①on the Base plate.
¾Make sure the Buffer Cartridge fits snugly and exactly into its place.
Misplacement of the Buffer Cartridge may result in instrument break
down and malfunction.
8c. Place the Elution tube rack on the Base plate.
¾Make sure the direction and location of the Elution tube rack is correct.
8d. Place the Disposable tip rack on the Base plate.
¾Make sure the direction and location of the Disposable tip rack is
correct.
8e. Place the Waste tray into the gap between Buffer Cartridges ①and ②.
8f. Push the Base Plate in completely and close the door.
¾Press the ‘ok’ button to complete.

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Fully Automated Nucleic Acid Extraction System
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V. DNA/ RNA Extraction (continued)
3. RUN (continued)
9. Verify the name of the target nucleic acid type and sample
source type (two-letter code: XX/XX) on the Running Mode
screen, and press the ‘RUN’ button.
¾Progress of the extraction run can be checked through the
progress bar on the lower portion of the LCD touchscreen.
10. You may press the ‘STOP’ button during the run to terminate
the extraction.
¾If you press ‘STOP’ during an extraction run, a popup prompt
asking you whether you are sure (‘Are you sure?’) will
appear. Select ‘Yes’ to terminate the run, or ‘No’ to cancel
the stop and proceed with the extraction run.
¾You may select ‘PAUSE’ to temporarily stop the run and
‘RUN’ to resume.
11. After the extraction run is complete, pull out the Base Plate and
remove the Elution tubes, Buffer Cartridges and all racks from
the Base Plate. After removing all accessories, push the Base
Plate back in completely and close the door.
12. You are given three options at this point:
¾Still remains same work: Repeat the current protocol.
¾Do other work: Perform an extraction run using a different
protocol for another nucleic acid and sample source type.
¾Finish: Finish and exit.
13. If the automatic UV-sterilization option is enabled, a popup
prompt will appear warning you not to open the door as UV-
sterilization is in progress. Refer to page 22 for details on
enabling automatic UV-sterilization.
14. Press the ‘START’ button to initiate sterilization
¾Select ‘SKIP’ if you wish to pass sterilization.
15. The sterilization process takes 5 minutes. Progress can be
checked through the progress bar.
※Upon completion of the extraction run, immediately close the Elution tube caps to prevent spillage and
label the tubes to avoid confusion later on.

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Fully Automated Nucleic Acid Extraction System
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VI. ExiPrep™16 Plus Setup
1. Main Menu
Main Menu
Once the initialization has completed successfully, the
LCD touchscreen will display the MENU as shown
below.
Please contact Bioneer Customer Service or your local
sales representative if the initialization progress bar
does not change for over 5 minutes during initialization
or if the MENU screen does not appear after
initialization.
Icon Details
DNA/ RNA extraction setting menu
¾Extract nucleic acids with ExiPrepTM16 Plus.
UV lamp ON/ OFF selection menu
¾Sterilize the internal cavity with the built-in UV lamp. Press the ‘UV lamp’
button to begin sterilization. The sterilization runs for 5 minutes. To cancel,
press the ‘UV lamp’ button again.
¾ExiPrepTM16 Plus provides an automatic UV sterilization option to run after
every DNA/ RNA extraction run. Details are described in page 22.
System setting menu
¾Set up or cancel the system. You could check out user registration or
cancelation, the setup of user restricted menu, password, and system, and
history easily. More detailed information is described in page 18.
Contamination protection accessory installation
¾This icon is to determine the status of the syringe block for setting the
contamination shield.
Power OFF button

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Fully Automated Nucleic Acid Extraction System
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VI. ExiPrepTM 16 Plus Setup (continued)
1. Main Menu (continued)
1) PREP SETUP
MENU screen PREP SETUP screen
Selecting ‘Start’ from the Main Menu will bring up the ‘PREP SETUP’ screen where
you can enter the three-digit code for the extraction and sample source type.
Refer to the code list within this Manual to select the three-digit code(29 page) applicable
to your desired nucleic acid and sample source type.

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Fully Automated Nucleic Acid Extraction System
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VI. ExiPrepTM 16 Plus Setup (continued)
1. Main Menu (continued)
2) UV sterilization (UV lamp)
Main Menu screen UV Sterilization screen
Use the built-in UV-lamp to sterilize the internal cavity of the instrument.
Press the ‘UV lamp’ icon to initiate the UV sterilization process. The icon will turn
yellow as UV sterilization proceeds.
The sterilization runs for 5 minutes. The progress can be tracked through the progress
bar displayed on the bottom portion of the LCD touchscreen. To cancel the sterilization
process, press the ‘UV lamp’ button again.
ExiPrepTM 16 Plus provides an automatic UV sterilization after every DNA/ RNA
extraction run. Details on this function are described in page 22.

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Fully Automated Nucleic Acid Extraction System
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VI. ExiPrepTM 16 Plus Setup (continued)
1. Main Menu (continued)
3) System setup menu (SETUP)
Main Menu Screen Setup Screen
Icon Description
User registration menu
¾You may create new accounts through this menu. Details on
account creation can be found in page 19.
System configuration menu
¾Allows you to restrict non-registered users from accessing
features such as UV sterilization and system preferences. Details
on system configuration can be found in page 23.
History
¾Enabled by selecting the user login option.
¾Allows you to audit up to 99 most recent runs by displaying
information such as user ID, operation record and the instrument
status (successful, cancelled) of a particular run.
¾Details can be found in page 20.
Self Test
¾This icon is for testing each motor initialization and heater block
Temperature.
Tip Out
¾This icon is for removing the Disposable Tips from the instrument
Syringe Block. Pressing this icon will release the tips
immediately.