Biosan CH-100 User manual

2
Contents
About this edition of the operating manual...............................................................2
Safety precautions ...................................................................................................3
General information..................................................................................................4
Getting started..........................................................................................................5
Operation .................................................................................................................7
Calibration..............................................................................................................10
Specification...........................................................................................................12
Maintenance...........................................................................................................13
Warranty and Claims. Registration.........................................................................14
EU Declaration of conformity..................................................................................15
About this edition of the operating manual
The manual applies to following model and versions of heating/cooling system:
CH-100 versions V.7A01, V.7A02 and V.7A03
CH 3-150 versions V.2AD and V.2AE

3
Safety precautions
The following symbols mean:
Caution! Make sure you have fully read and understood the present Manual
before using the equipment. Please pay special attention to sections
marked by this symbol.
Caution! Surfaces can become hot or cold during use.
GENERAL SAFETY
Use only as specified in the Operating Manual provided.
Protect the unit from shocks or falling.
Store and transport the unit at ambient temperatures between -20°C and +60°C and
maximum relative humidity of 80%.
After transportation or storage, keep the unit under room temperature for 2-3 h before
connecting to electric circuit.
Before using any cleaning or decontamination methods except those recommended
by the manufacturer, check with the manufacturer that the proposed method will not
damage the equipment.
Do not make modifications to the design of the unit.
ELECTRICAL SAFETY
Connect only to external power supply (CH-100) or electric circuit (CH 3-150) with
voltage corresponding to that on the serial number label.
Use only the external power supply (CH-100) provided with the unit.
Do not use an ungrounded power socket or an ungrounded extension lead.
Ensure that the switch and the plug are easily accessible during use.
If liquid penetrates into the unit, disconnect it from electric circuit and have it checked
by a repair and maintenance technician.
Disconnect the unit from electric circuit before moving.
Do not operate the unit in premises where condensation can form. Operating
conditions of the unit are defined in the Specification section.
DURING OPERATION
Use only tubes of standard size.
Do not check the temperature by touch. Use a thermometer.
(For model CH 3-150) Remove the blocks only using the included extractor tool.
Do not operate the unit in environments with aggressive or explosive chemical
mixtures. Please contact manufacturer for possibility of unit operation in specific
atmosphere.
Do not operate the unit if it is faulty or has been installed incorrectly.
Do not use outside laboratory rooms.
Do not leave the operating unit unattended.
BIOLOGICAL SAFETY
It is the user’s responsibility to carry out appropriate decontamination if hazardous
material is spilt on or penetrates into the equipment.

4
General information
CH-100 and Combitherm-2 CH 3-150, dry-block thermostats with heating and cooling
function, are designed to thermally stabilize materials at a wide range of temperatures. Built-in
Peltier elements allow fast and precise cooling, and heating plates –heating of the materials
according to used methods.
Model CH-100 with built-in blocks (on request) is intended for micro quantities of
reagents, in volume range from 0.5 to 2.0 ml. Temperature setting range is from -10°C to
+100°C. Model features exceptional temperature maintenance stability and uniformity, as well
as high heating and cooling speed.
Model Combitherm-2 CH 3-150 with changeable blocks is designed for increased
versatility. Temperature setting range is from -3°C to +20°C for cooling block and from +25°C
to +150°C for heating block. Combitherm-2 consists of two independent modules for
interchangeable blocks,cooling and heating,combined ina single unit. Control panel isdivided
in two parts for separate control of cooling and heating blocks. Blocks are controlled
independently, with possibility of setting up to 16 custom programs for different temperature
and thermostating time. Seven thermoblocks are available, for different size and quantity of
tubes. Models can be used in:
Molecular and cell biology, for nucleic acid extraction and analysis.
Protein analysis (denaturation).
Biochemistry, for analysis of enzyme processes.
Chemical laboratories, in matter composition analysis.
Food and environmental investigation laboratories, in composition analysis of food
and cosmetics.
Depending on the model, different quantities of microtubes of different volumes can
be placed in the thermostat. Table 1. Block capacity, by model
Model and block
Capacity
Tube types
Model CH-100
CH-1
20 / 12
0.5 / 1.5 ml Eppendorf-type microtubes
CH-2
20
1.5 ml Eppendorf-type microtubes
CH-3
20
2 ml Eppendorf-type microtubes
Model CH 3-150
B23-1.5
23
1.5 ml Eppendorf-type microtubes
B18-12
18
Ø12 mm tubes
B10-13
10
Ø13 mm tubes
B10-16
10
Ø16 mm tubes
B6-25
6
Ø25 mm tubes
B5-29
5
Ø29 mm flasks
B2-50
2
Ø50 mm beakers

5
Getting started
4.1. Unpacking.
Remove packing materials carefully and retain them for future shipment or storage of
the unit. Examine the unit carefully for any damage incurred during transit. The
warranty does not cover in-transit damage. Warranty covers only the units
transported in the original package.
4.2. Complete set. Package contains:
4.2.1. CH 3-150 Combitherm-2
- CH 3-150 Combitherm-2 heating/cooling dry block system .............................1 pce.
- Transparent block lids......................................................................................2 pcs.
- Spare fuse (inside fuse holder) .......................................................................1 pce.
- Power cable .....................................................................................................1 pce.
- Operating manual, declaration of conformity ..................................................1 copy
- B23-1.5 block ........................................................................................on request
- B18-12 block .........................................................................................on request
- B10-13 block .........................................................................................on request
- B10-16 block .........................................................................................on request
- B6-25 block ...........................................................................................on request
- B5-29 block ...........................................................................................on request
- B2-50 block ...........................................................................................on request
- Extractor tool ...................................................................... 1 pce. with each block



6
4.2.2. CH-100
- CH-100 heating/cooling dry-block system........................................................1 pce.
- Transparent block lid........................................................................................1 pce.
- External power supply......................................................................................1 pce.
- Power cable .....................................................................................................1 pce.
- Operating manual, Certificate..........................................................................1 copy
4.3. Setup.
- Place the unit upon even horizontal non-flammable surface at least 20 cm away
from any flammable materials;
- Do not place any objects 40 cm behind the unit to ensure unimpeded air circulation;
- Remove protective film from the display;
- (Model CH 3-150) Plug the power cable into the socket on the rear side of the unit,
and position the unit with easy access to the power switch and plug.
- (Model CH-100) Connect the external power supply and the power cable. Plug the
external power supply into the socket on the rear side of the unit and position the
unit with easy access to the power switch and plug.
4.4. Heating or cooling block installation and replacement (model CH 3-150).
Caution! Heating/cooling block surface may become very hot or cold during
the operation. Avoid touching the block or heating/cooling surfaces.
Change the blocks only at room temperature.
- Use the extractor tool for removing blocks. Screw the tool into a threaded hole in
the middle of a heating/cooling block and lift it out. Unscrew the tool.
- Ensure that the heater bed and the block are clean in order to ensure good thermal
contact between unit and the block, resulting in optimal temperature control
performance.

7
Operation
Recommendations during operation
Please check the tubes before using, be sure that tubes are heat resistant. Don’t heat
the tubes over the melting point of the material they are made of. Remember that
thin-walled tubes have a higher thermoconducting factor.
Microtube caps can open under the action of high temperature (> 85°C), thus causing
sample volume shrinkage or potential health risk when working with infected material.
To prevent such cases, use microtubes with cap lock of Safe-Lock®type.
Do not fill tubes more than 3-5 mm over the level they are immersed in the heat block.
5.1. Working with model CH-100.
Fig. 1. Control panel of CH-100
5.1.1. Connect the external power supply to a grounded power socket. Switch on (position I)
the power switch on the rear panel of the unit.
5.1.2. The unit will turn on and the following readouts will be shown on the display:
- In the upper line Set: previously set time and temperature parameters;
- In the lower line Actual: operation status (S –stopped), timer indication STOP and
current temperature.
5.1.3. Temperature setting. Use the ▲ and ▼ T (ºC) keys (fig. 1/5) to set the required
temperature. Values are shown on display (upper line, fig. 1/3). Temperature incre-
ment is 0.1ºC. Pressing the key down for more than 2 s makes the values change
quicker.
5.1.4. Press the RUN/STOP T (ºC) key (fig. 1/8) to start heating or cooling. The corres-
ponding operation status is indicated on the display, Hfor heating, Cfor cooling
(fig. 1/1). Current temperature value is shown on display (lower line, fig. 1/5).
5.1.5. To stop the heating or cooling, press the RUN/STOP T (ºC) key again. It takes a few
moments before the heating or cooling process stops and the operation mode
indicator shows S, for stopped.
5.1.6. The set temperature can be changed during operation without stopping the process.
5.1.7. After thermal stabilisation of the unit (i.e. after set and current temperatures equalize,
fig. 1/3), open the block lid, place samples and close the lid.
5.1.8. Timer setting. The unit is equipped with an independent timer for convenient control
over the samples heating/cooling time.
5.1.9. Use the ▲ and ▼ TIME keys (fig. 1/4) to set the required time period. Values are
shown on display (upper line, fig. 1/2). Time increment is 1 minute. Pressing the key
down for more than 2 s makes the values change quicker.
5.1.10.Press the RUN/STOP TIME key (fig. 1/7) once to start the timer. The elapsed time is
indicated in the lower line of the display (fig.1/2). When the set time is reached, the
timer stops and a sound signal is heard.

8
Caution! After the set time elapses and the timer stops, the device does not
stop the heating or cooling process. Press the RUN/STOP T (ºC) key
(fig. 1/7) manually to stop the heating or cooling.
5.1.11.The timer can be stopped before the set time elapses if required by pressing the
RUN/STOP TIME key. Pressing the key again restarts the timer.
5.1.12.The set time value can be changed during operation without stopping the timer.
5.1.13.After finishing the operation, turn off (position O) the unit switching off the power
switch on the rear panel. Disconnect the external power supply from electric circuit.
5.2. Working with model CH 3-150.
Fig. 2. Control panel of CH 3-150
5.2.1. Connect the power cord to a grounded power socket and switch on (position I) the
power switch located on the rear panel of the unit.
5.2.2. The unit will switch on and the following readouts will be shown on the display:
- In the upper line Set p.: previously set time and temperature parameters;
- In the lower line Actual p.: program indication STOP and current temperature.
Caution! Temperature starts changing automatically after switching on
according to selected settings. Time of temperature stabilization
depends on the initial temperature.
5.2.3. The unit has 16 cooling and 16 heating programs for temperature and time setup.
One cooling and three heating programs are factory pre-set. The programs have
following settings:
Program
Temperature
Time, hh:mm
Program
Temperature
Time, hh:mm
Cooling [P1]
-3 °C
1:00
Heating [P1]
+80 °C
0:30
Cooling [P2-16]
-3 °C
0:00
Heating [P2]
+105 °C
0:07
Heating [P3]
+150 °C
2:00
Heating [P4-16]
+25 °C
0:00

9
5.2.4. Selecting a program. Press the PCkey (fig. 2/6) to select cooling programs or the
PHkey (fig. 2/9) to select heating programs. Each press selects next program in cycle.
5.2.5. Changing current program parameters. All 32 programs can be changed.
Note. Holding the key pressed for more than 2 seconds increases the
parameter change speed.
Note. The settings cannot be changed during operation of the timer.
5.2.5.1Cooling programs. Press and hold the PCkey for 4 seconds until <symbol appears
on the display (fig. 2/1).
Use the ▲ and ▼ Temp. keys (fig. 2/4) to set the required temperature. Cooling
increment is 0.1ºC. Set values are shown on display on the left in the upper line, Set
p. (fig. 2/2).
Use the ▲ and ▼ Time keys (fig. 2/5) to set the required work time interval in hours and
minutes. Time increment is 1 minute. Set values are shown on display on the left in the
upper line, Set p. (fig. 2/3).
Press the PCkey to save settings and return to work mode.
5.2.5.2Heating programs. Press and hold the PHkey for 4 seconds until >symbol appears
on the display (fig. 2/14).
Use the ▲ and ▼ Temp. keys (fig. 2/11) to set the required temperature. Heating
increment is 1ºC. Set values are shown on display on the right in the upper line, Set
p. (fig. 2/13).
Use the ▲ and ▼ Time keys (fig.2/10) to set the required work timeintervalin hours and
minutes. Time increment is 1 minute. Set values are shown on display on the right in the
upper line, Set p. (fig. 2/12)
Press the PHkey to save settings and return to work mode.
5.2.6. Program execution. When the necessary program is selected with PCor PHkey,
heating or cooling processes start automatically.
5.2.7. After thermal stabilisation of the unit (i.e. after set and current temperatures equalize),
open the heating or cooling block lid, place samples and close the lid.
Caution! Surfaces can become hot or cold during use. Use of protective cotton
gloves is advised.
5.2.8. Press the Start Stop key (fig. 2/7 or 2/8) to start the cooling or heating timer.
5.2.9. After the set time elapses, timer stops, display shows the flashing STOP indication
and the unit makes a repeating sound signal. Press the corresponding Start Stop
key to turn off the signal.
Caution! Stopping the timer doesnot stop the heating andcooling process. Set
the temperature higher than 20 °C when cooling or lower than 25 °C
when heating, with ▲ and ▼ Temp keys.
5.2.10.The running timer can be stopped at any time by pressing the Start Stop key.
Pressing Start Stop key again restarts the timer.
5.2.11.After finishing the operation, turn off the unit by setting the power switch on the rear
panel to position O(off) and disconnect the power cord from electric circuit.

10
Calibration
6.1. The device is precalibratedat the factory (calibrating coefficient is1.000) foroperation
with temperatures measured by a sensor in the heating block.
6.2. To change the calibration coefficient, hold the RUN/STOP TIME key (fig. 1/7, CH-
100) or Start Stop (fig. 2/7, CH 3-150) key pressed for more than 8 s to activate
calibration mode. The calibration mode appears on the display (fig. 3).
6.3. Restoring factory settings. Set 1.000 value using the ▲ and ▼ Temp or T (ºC)
keys as shown on fig. 3/1 to restore the factory settings. Press the RUN/STOP TIME
or Start Stop key once to save the changes and exit the calibration mode.
Note. For model CH 3-150, coefficient value changes are recommended
after the unit has reached 30°C temperature.
6.4. Calibration procedure.
6.4.1. Install independent sensor (0.5°C accuracy) into microtubes placed into the block
sockets.
6.4.2. Set the required temperature in operation mode (e.g. 40°C).
Fig. 3. Control panel in calibration mode
6.4.3. After the unit reaches the set temperature (when the set and current temperature
readings equal) leave the unit for 30 min for thermal stabilization.
6.4.4. Let us assume that the readings of independent sensor is 39°C, but the display’s
actual temperature is 40°C (fig. 3/4). Then it is necessary to add 1°C correction.
6.4.5. Hold the RUN/STOP TIME or Start Stop key pressed for more than 8 s to activate
calibration mode. The following parameters will be shown on the display (fig. 3).
6.4.6. Using the ▲ and ▼ Temp or T (ºC) keys, change the calibration coefficient (fig. 4/1)
so that the new temperature value (fig. 4/2) corresponds to the independent sensor
temperature. In our example, the calibration coefficient will be 0.974.
Note. Calibration coefficient can be changed in range from 0.936 to 1.063
with increment of 0.001. This calibrating coefficient will correct
temperature through all the operation range of the block.
Note. For model CH 3-150, coefficient value changes are recommended
after the unit has reached 30°C temperature.
6.4.7. After finishing the calibration, press the RUN/STOP TIME or Start Stop key once to
save the changes and exit the calibration mode.
6.4.8. The display will show calibrated temperature as shown on fig. 5/1 and the unit will
continue thermal stabilization according to the previously set temperature.

11
Fig. 4. Control panel in calibration and operation mode
Fig. 5. Control panel in operation mode after calibration

12
Specification
The unit is designed for operation in cold rooms, incubators (except CO2incubators)
and laboratory rooms at ambient temperature from +4C to +40°C in a non-condensing
atmosphere and maximum relative humidity 80% for temperatures up to 31°C decreasing
linearly to 50% relative humidity at 40°C.
Temperature specifications
CH-100
CH 3-150
Setting range
Cooling
-10°C … +100°C
-3°C … +20°C
Heating
+25°C … +150°C
Control range
Cooling
30°C below RT*… +100°C
23°C below RT …
5°C below RT
Heating
5°C above RT … +150°C
Setting resolution
Cooling
±0.1°C
±0.1 °C
Heating
±1 °C
Stability
±0.1°C
Uniformity at +37°C
±0.1°C
Heat up time from 25°C to 100°C
16 min
12 min
Cool down time from 25°C
21 min (to -10°C)
60 min (to -3°C)
Temperature calibration option
yes
Calibration
coefficient range
Heating
0.936 … 1.063 (±0.063)
0.936 … 1.063 (±0.063)
Cooling
-
General specifications
CH-100
CH 3-150
Digital time setting
1 min –96 h
1 min –99 h 59 min
Digital time setting increment
1 min
LCD Display
2x16 symbols
2x24 symbols
Dimensions
240x260x165 mm
295x285x220 mm
Weight**
3.2 kg
5.6 kg
Power consumption
60 W
430 W
Input current
DC 12 V, 5.0 A
AC 230 V, 50 Hz, 1.9 A or
AC 120 V, 60 Hz, 3.6 A
External power supply
In AC 100-240 V, 50/60 Hz
Out DC 12 V
-
Table 2. Built-in block capacity in CH-100
Built-in block
Capacity
Catalogue number
CH-1
20 x 0.5 ml and 12 x 1.5 ml microtubes
BS-010410-BAI
CH-2
20 x 1.5 ml microtubes
BS-010410-BAI
CH-3
20 x 2 ml microtubes
BS-010410-BAI
* Room temperature
** Accurate within ±10%.

13
Table 3. Exchangeable block capacity in CH 3-150
Block
model
Description
Form of block
socket bottom
Catalogue number
B23-1.5
23 sockets for 1.5 ml microtubes
Conical
BS-010418-DK
B18-12
18 sockets Ø12 mm, depth 58 mm
Round
BS-010418-EK
B10-13
10 sockets Ø13 mm, depth 30 mm
Flat
BS-010418-LK
B10-16
10 sockets Ø16 mm, depth 56 mm
Flat
BS-010418-BK
B6-25
6 sockets Ø25 mm, depth 40 mm
Flat
BS-010418-CK
B5-29
5 sockets Ø29 mm, depth 40 mm
Flat
BS-010418-KK
B2-50
2 sockets Ø48 mm, depth 58 mm
Flat
BS-010418-AK
Biosan is committed to a continuous program of improvement and reserves the right
to alter design and specifications of the equipment without additional notice.
Maintenance
8.1. If the unit requires maintenance electric circuit, disconnect the unit from electriccircuit
and contact Biosan or your local Biosan representative.
8.2. All maintenance and repair operations must be performed only by qualified and
specially trained personnel.
8.3. Standard ethanol solution (75%) or other cleaning agents recommended for cleaning
of laboratory equipment can be used for cleaning and decontamination of the unit.
8.4. Fuse replacement (model CH 3-150)
- Disconnect from electric circuit.
- Remove the power plug from the rear side of the unit.
- Pull out the fuse holder by applying leverage in recess
(fig. 6/A).
- Remove the fuse from the holder.
- Check and replace with the correct fuse if necessary, M2
A for 230 V or M 3.15 A for 120 V, (type M- time lag:
Medium).
Fig. 6. Fuse replacement
8.5. Error codes in case of a defect.
Some malfunctions trigger an error code to appear on display, accompanied by a
sound signal every 8 s. Press RPM RUN/STOP key to turn off the signal. Error code
format is letters ER and a number from 1to 5.
Disconnect the unit from the electric circuit and report the error code to Biosan or
your local Biosan representative.

14
Warranty and Claims. Registration
9.1. The manufacturer guarantees the compliance of unit with the requirements of
specifications, if the customer follows the operation, storage and transportation
instructions.
9.2. The warranted service life of unit from date of delivery to the customer is 24 months.
For extended warranty, see p. 9.5.
9.3. Warranty covers only the units transported in the original package.
9.4. If any manufacturing defects are discovered by the Customer, an unsatisfactory
equipment report shall be compiled, certified and sent to the local distributor address.
To obtain the claim form, visit section Technical support on our website at link
below.
9.5. Extended warranty.
For CH 3-150, a Premium class model, one year of extended warranty is available
free of charge after registration, during 6 months from the date of sale. Online
registration form can be found in section Warranty registration on our website at
the link below.
For CH-100, a Basic Plus class model, extended warranty is a paid service. Contact
your local Biosan representative or our service department through the Technical
support section on our website at the link below.
9.6. Description of the classes of our products is available in the Product class de-
scription section on our website at the link below.
Technical support
Warranty registration
Product class description
biosan.lv/en/support
biosan.lv/register-en
biosan.lv/classes-en
9.7. The following information will be required in the event that warranty or post-warranty
service comes necessary. Complete the table below and retain for your records.
Model
CH-100 / CH 3-150 Combitherm-2
Dry block heating/cooling system
Serial number
Date of sale

15
EU Declaration of conformity
Unit type Dry block thermostats
Models Bio TDB-100, TDB-120, CH-100, CH3-150,
DB-4S, DB-10C
Serial number 14 digits styled XXXXXXYYMMZZZZ, where XXXXXX is
model code, YY and MM –year and month of production,
ZZZZ –unit number.
Manufacturer SIA BIOSAN
Latvia, LV-1067, Riga, Ratsupites str. 7/2
Applicable Directives EMC Directive 2014/30/EU
LVD Directive 2014/35/EU
RoHS2 2011/65/EU
WEEE 2012/19/EU
Applicable Standards LVS EN 61326-1: 2013
Electrical equipment for measurement, control and
laboratory use. EMC requirements. General requirements.
LVS EN 61010-1: 2011
Safety requirements for electrical equipment for
measurement, control, and laboratory use. General
requirements.
LVS EN 61010-2-010: 2015
Particular requirements for laboratory equipment for the
heating of materials.
We declare that this product conforms to the requirements of the above Directives
____________________ ____________________
Signature Signature
Svetlana Bankovska Aleksandr Shevchik
Managing director Engineer of R&D
____________________ ____________________
Date Date

Biosan SIA
Ratsupites 7, build.2, Riga, LV-1067, Latvia
Phone: [phone] Fax: [phone]
http://www.biosan.lv
Edition 2.-7.01 –February 2017