Zeiss Auto Focus User manual

Operating Manual
Auto Focus equipment

COPYRIGHT
Auto Focus equipment Carl Zeiss
06/2013 425131-7044-001 3
Prior knowledge of this manual is essential for proper operation of the device. You are thus advised to
familiarize yourself with its contents and, equally importantly, to follow the special notes and instructions
regarding safe handling of the device.
We reserve the right to make changes as deemed appropriate in the interests of ongoing technical
improvement. This manual is not subject to updating or revision services of any kind.
© This document may not be passed on to a third person, nor may its contents be duplicated, used or
disseminated in any other way unless express approval to do so has been obtained. Offenders will
be held liable for the payment of damages.
All rights, including rights created by patent grant or registration of a utility model or design, are
reserved.
All company or product names mentioned in this operating manual may be trademarks or
registered trademarks. References to products of other manufacturers are provided for information
only. They are not intended to express approval or recommendation for use of such products.
Carl Zeiss Microscopy GmbH will not assume any liability as to the performance or the use of such
products.
Editor: Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
microscop[email protected]m
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Königsallee 9-21
37081 Göttingen, Germany
Order number: 425131-7044-001
Date of issue: Version 7, June 21, 2013

TABEL OF CONTENTS
Carl Zeiss Auto Focus equipment
4 425131-7044-001 06/2013
TABLE OF CONTENTS
Page
1INTRODUCTION............................................................................................................7
1.1 Notes regarding operational safety..................................................................................7
1.2 Warning and information labels ....................................................................................10
1.3 Notes on warranty........................................................................................................10
2PRODUCT DESCRIPTION.............................................................................................11
2.1 Product designation, intended use ................................................................................11
2.2 Restrictions...................................................................................................................11
2.3 General view of components ........................................................................................12
2.4 Compatible Objectives for use with Auto Focus .............................................................13
2.5 Selectable stages ..........................................................................................................15
2.6 Functionality.................................................................................................................16
2.7 Technical data ..............................................................................................................17
2.7.1 Dimensions and weight ................................................................................................17
2.7.2 Ambient conditions ......................................................................................................17
2.7.3 Operating data .............................................................................................................18
3INSTALLATION ...........................................................................................................19
3.1 Connecting Auto Focus ................................................................................................19
3.2Camera deflection ........................................................................................................21
3.3 Auto Focus objectives ...................................................................................................21
3.4 Insert damping filter .....................................................................................................21
4OPERATION ................................................................................................................22
4.1 Switching on/off...........................................................................................................22
4.2 Setting up Auto Focus ..................................................................................................23
4.3Auto Focus operation via the TFT display .......................................................................27
4.4 Activate / Deactivate Auto Focus ...................................................................................28
4.5 Auto Focus in "On" and "Once" status.........................................................................29
4.6 Auto Focus in "Standby" status ....................................................................................29
4.7 Set Load Position ..........................................................................................................30
4.8 Setting focus reference values.......................................................................................31
5MAINTENANCE, REPLACEMENT OF FUSES AND SERVICE.........................................32
5.1 Maintenance ................................................................................................................32
5.2 Replacement of fuses in the fc12 Auto Focus controller .................................................32
5.3 Troubleshooting ...........................................................................................................33
5.4 Error messages and typical background situations..........................................................34

CONTENTS
Auto Focus equipment Carl Zeiss
06/2013 425131-7044-001 5
5.5 Other fault events ........................................................................................................ 35
5.6 Service......................................................................................................................... 36
6INTELLECTUAL PROPERTY......................................................................................... 37
7INDEX......................................................................................................................... 38

TABEL OF CONTENTS
Carl Zeiss Auto Focus equipment
6 425131-7044-001 06/2013
LIST OF ILLUSTRATIONS
Page
Fig. 1 Warning and information labels.........................................................................................10
Fig. 2 General view of Auto Focus components ...........................................................................12
Fig. 3 Function diagram of Auto Focus ........................................................................................16
Fig. 4 Auto Focus working modes ...............................................................................................16
Fig. 5 Connection diagram for Auto Focus and controller to the Axio Imager 2 stand ...................19
Fig. 6 Auto Focus sensor module.................................................................................................20
Fig. 7 Rear view of controller and connections.............................................................................20
Fig. 8 Manual camera deflection (425103-0000-000) for left side port .........................................21
Fig. 9 Front view of controller .....................................................................................................22
Fig. 10 TFT display, Home screen with Auto Focus status ...............................................................23
Fig. 11 TFT display, Auto Focus settings.........................................................................................23
Fig. 12 TFT display, Microscope / xyz / Auto Focus / Options / Period ..............................................24
Fig. 13 TFT display, options in the Auto Focus tab..........................................................................27
Fig. 14 Display forms of the status icons for Auto Focus on the TFT display in On / Standby status
(left and central) and visualization of error messages. .........................................................28
Fig. 15 Replace the fuses in the controller .....................................................................................32

INTRODUCTION
Auto Focus equipment Notes regarding operational safety Carl Zeiss
06/2013 425131-7044-001 7
1INTRODUCTION
1.1 Notes regarding operational safety
Auto Focus has been designed, manufactured and tested to comply with DIN EN 61010-1 (IEC 61010-1)
Safety requirements for electrical equipment for measurement, control and laboratory use.
The device complies with EC Directives 2006/95/EC, Low Voltage Directive and 2004/108/EC,
Electromagnetic Compatibility. The device must be disposed of in accordance with the WEEE Directive
2002/96/EC.
Auto Focus carries a mark.
This operating manual contains information and warnings that must be followed by the owner/operator
personnel.
The warnings and advisory notes used throughout this operating manual have the following meanings:
WARNING
This symbol indicates a potential hazard to the user.
WARNING
This symbol indicates a potential danger of IR radiation exposure created by operator action.
WARNING
Disconnect mains plug before opening the device!
CAUTION
This symbol indicates a potential hazard to the instrument or system.
NOTE
This symbol designates information that should be closely followed.
STANDBY
The Auto Focus should not be used in any other way than as described in this operating manual. The
microscope manual should also be consulted where appropriate.

INTRODUCTION
Carl Zeiss Notes regarding operational safety Auto Focus equipment
8 425131-7044-001 06/2013
The following notes should be strictly observed:
Class 3B IR radiation, invisible LED radiation while the sensor module is open. Do not expose eyes
to IR radiation.
The Auto Focus sensor module does not include any special facilities providing protection from
samples with a corrosive, potentially infectious, toxic, radioactive or other health-damaging
effect. You are under obligation to comply with all legal requirements, in particular national
accident prevention regulations, when handling samples of this kind.
Dirt or dust may adversely impact the device’s operating capability. For this reason, steps must be
taken to eliminate such influences as much as possible.
The device may not be opened except by trained personnel or service technicians. The
Auto Focus may not be operated in an explosive atmosphere.
Always pull the main power plug before removing the device or replacing a fuse! Refer to
section 5.2 on page 32.
Only fuses of a type specified in the technical data section may be used. The use of makeshift
fuses and shorting of fuse holders is prohibited.
The manufacturer cannot accept liability if the device, or individual assemblies or parts thereof,
have been used for any other purpose. The same will apply if service or repair work of any kind
is not performed by authorized service personnel. In this ca
se all rights to claim
warranty / guarantee will be null and void.
The main power plug may only be inserted into a socket with PE contact, and its built-in
protection must not be rendered ineffective by using a cable extension without a PE conductor.
Should it be determined that protective measures are no longer effective, shut off the power to
the device and take precautions to ensure that it cannot be inadvertently switched on again.
Contact the Zeiss Customer Service or Carl Zeiss Microscopy Service to have the device repaired.
Clogged or covered ventilation slots may result in heat build-up with a damaging effect on the
device. Always keep the ventilation slots unobstructed and refrain from inserting or dropping
objects into a ventilation slot.
A defective controller does not classify as domestic waste. It must be disposed of in accordance
with currently valid provisions of law.
Likewise, samples must be disposed of in accordance with currently valid provisions of law and
internal work instructions.

INTRODUCTION
Auto Focus equipment Notes regarding operational safety Carl Zeiss
06/2013 425131-7044-001 9
Devices may only be operated by duly instructed personnel who must have received proper
instructions about the potential danger involved in operation of the Auto Focus. The focus-
maintaining controller, which controls the focus drive, is a
precision instrument. It may suffer
impaired functionality or even destruction as a result of unqualified intervention.
The detachable power cables may not be replaced by cables of inadequate dimensions.
The fc12 focus controller is designed to work within a voltage range of 100 V to 240 V ±10 %
at 50 to 60 Hz without additional voltage conversion.
Voltage to the Axio Imager 2 is supplied from the separate internal power supply or from the
VP232-2 power supply which is an integral part of the stand. Voltage to the Axio Imager Vario is
supplied from the VP232-2 power supply which is an integral part of the stand.
No voltage conversion is required for operation in a voltage range of 100 V to 240 V ±10
%, at
frequencies from 50 to 60 Hz.
Switching the Auto
Focus controller to the "Off" position will only turn off the internal
computer. The mains voltage of the controller or the Auto Focus sensor will not be disconnected.
To interrupt the mains supply, the main power plug must be pulled.

INTRODUCTION
Carl Zeiss Warning and information labels Auto Focus equipment
10 425131-7044-001 06/2013
1.2 Warning and information labels
1.3 Notes on warranty
The manufacturer warrants this device to be free from faults in material and workmanship at the time of
delivery. Any defects occurring must be notified immediately upon detection and maximum efforts must
be undertaken to restrict the damage to a minimum. Upon receiving notice of a defect, the manufacturer
will be under obligation to remedy it at his own discretion, either by repairing the device or delivering a
faultless replacement. No warranty will be accepted in cases of natural wear (in particular wearing parts)
or improper handling.
The manufacturer will refuse any liability for damage resulting from incorrect operation, negligence or
unauthorized intervention in the device, in particular from the removal or replacement of device parts or
use of accessory parts from other manufacturers. Any such case will void the user’s claim for warranty.
Except for activities explicitly described in this operating manual, no maintenance or repairs of any kind
may be performed on the microscopes. Only Zeiss Customer Service personnel or specifically authorized
representatives are allowed to handle repair work.
In the event of a malfunction, contact the Carl Zeiss Microscopy Service in Germany (see page 36) or the
responsible Carl Zeiss service agency.
Fig. 1 Warning and information labels

PRODUCT DESCRIPTION
Auto Focus equipment Product designation, intended use Carl Zeiss
06/2013 425131-7044-001 11
2PRODUCT DESCRIPTION
2.1 Product designation, intended use
Manufacturer’s name: Auto Focus
The Auto Focus is designed for use with the Axio Imager 2 and Axio Imager Vario stands with motorized
focus. The left camera deflection port serves as the point of coupling.
Auto Focus allows the focal plane to be found automatically and quickly on the sample surfaces and to
be maintained when moving the samples along the X or Y axis, or to be adjusted in line with the surface
structure.
The focus adjustment is carried out automatically by the focus controller, which controls the motorized
focus drive (Z axis) of the microscope.
Use of Auto Focus is recommended for reflective samples such as semiconductors or metals. It is
distinguished by its considerably shorter control time in comparison to the software autofocus function
and its wide capture range of several hundred depths of field.
The suitability of Auto Focus should be checked for all other surface properties (e.g. partially reflective,
diffuse). A selection of different samples / surface properties can be found in Table 3 on page 26.
2.2 Restrictions
The capture range and the accuracy of Auto Focus (see Table 2, page 15) should be taken into
consideration for the different objective magnifications. These differ depending on the system
configuration, work mode and sample surface. The maximum capture range of Auto Focus (see Table 2,
page 15) has been set using highly reflective samples.
Optimum quality of the Auto Focus signal can only be guaranteed if the special left camera deflection for
the Auto Focus is used with the appropriate Auto Focus installation set.
Use of the camera deflection prevents use of the Analyzer slider (fixed or rotatable). For transmitted light
applications requiring a rotating polarizer / analyzer, the rotating polarizer (427706-0000-000) and the
P&C reflector module (424941-9050-000) can be used, and for reflected light applications, the rotating
polarizer (427704-9901-000) and the P&C reflector module (424941-9050-000) can be used.
The DIC / Pol ACR P&C reflector module (424939-0000-000) can be used for reflected light polarization
applications with fixed polarizers and for DIC applications.
Use of the brightfield reflector module (424928-9901-000) is only possible without grey filter.
Use of the Auto Focus equipment may be limited with polarization applications, especially at greater
magnifications.
Use of Auto Focus may be limited with DIC applications.
Use of Auto Focus with C-DIC and fluorescence applications is not possible.
Use of a linear focus sensor (430703-0000-000) for Axio Imager 2 and for Axio Imager Vario (425300-
9000-000) in combination with Auto Focus is not possible.

PRODUCT DESCRIPTION
Carl Zeiss General view of components Auto Focus equipment
12 425131-7044-001 06/2013
2.3 General view of components
The Auto Focus components include:
•Auto Focus equipment with controller (425131-9000-000)
•Auto Focus installation set (425131-9030-000)
Also required:
•camera deflection (side port) left, manual (425103-0000-000) or successor
or
•camera deflection (side port) left, motorized (425104-0000-000) or successor
The following firmware and software versions (or newer versions)
are needed to operate the
Auto Focus on the Axio Imager 2 and Axio Imager Vario 2 stands:
−Axio Imager 2: 4.051, 4.551
−Axio Imager Vario 2: 5.536
−MTB2011: 2.1.0.8
−AxioVision: DVD 48 Release 4.9.1
It is not currently possible to operate the Auto Focus with ZEN.
Fig. 2 General view of Auto Focus components

PRODUCT DESCRIPTION
Auto Focus equipment Compatible Objectives for use with Auto Focus Carl Zeiss
06/2013 425131-7044-001 13
2.4 Compatible Objectives for use with Auto Focus
Series Objective Item number
EC Epiplan EC Epiplan 5x/0.13 H W0.8 442020-9901-000
EC Epiplan 5x/0.13 H M27 422030-9901-000
EC Epiplan 5x/0.13 H M27 422030-9902-000
EC Epiplan 5x/0.13 HD M27 422030-9960-000
EC Epiplan 5x/0.13 HD M27 422030-9961-000
EC Epiplan 10x/0.2 H W0.8 442030-9902-000
EC Epiplan 10x/0.2 H M27 422040-9901-000
EC Epiplan 10x/0.25 H M27 422040-9902-000
EC Epiplan 10x/0.2 HD M27 422040-9960-000
EC Epiplan 10x/0.25 HD M27 422040-9961-000
EC Epiplan 10x/0.2 Pol W0.8 442033-9901-000
EC Epiplan 10x/0.2 Pol M27 422043-9901-000
EC Epiplan 10x/0.25 Pol M27 422043-9902-000
EC Epiplan 20x/0.4 H W0.8 442040-9901-000
EC Epiplan 20x/0.4 H M27 422050-9902-000
EC Epiplan 20x/0.4 H M27 422050-9903-000
EC Epiplan 20x/0.4 HD M27 422050-9960-000
EC Epiplan 20x/0.4 HD M27 422050-9961-000
EC Epiplan 40x/0.6 M27 422060-9901-000
EC Epiplan 40x/0.6 HD M27 422060-9961-000
EC Epiplan 50x/0.7 H W0.8 442060-9900-000
EC Epiplan 50x/0.7 H M27 422070-9901-000
EC Epiplan 50x/0.75 H M27 422070-9902-000
EC Epiplan 50x/0.7 HD M27 422070-9960-000
EC Epiplan 50x/0.75 HD M27 422070-9961-000
EC Epiplan 50x/0.7 Pol W0.8 442063-9900-000
EC Epiplan 50x/0.7 Pol M27 422073-9901-000
EC Epiplan 50x/0.75 Pol M27 422073-9902-000
EC Epiplan 100x/0.8 H W0.8 442080-9900-000
EC Epiplan 100x/0.8 H M27 422090-9901-000
EC Epiplan 100x/0.85 H M27 422090-9902-000
EC Epiplan 100x/0.8 HD M27 422090-9960-000
EC Epiplan 100x/0.85 HD M27 422090-9961-000
EC Epiplan-
Apochromat
EC Epiplan-Apochromat 10x/0.3 HD DIC M27 422642-9960-000
EC Epiplan-Apochromat 20x/0.6 HD DIC M27 422652-9960-000
EC Epiplan-Apochromat 50x/0.95 HD DIC M27 422672-9960-000
EC Epiplan-Apochromat 100x/0.95 HD DIC M27 422692-9960-000

PRODUCT DESCRIPTION
Carl Zeiss Compatible Objectives for use with Auto Focus Auto Focus equipment
14 425131-7044-001 06/2013
Series Objective Item number
EC Epiplan-
Neofluar
EC Epiplan-Neofluar 1.25x/0.03 M27 422310-9900-000
EC Epiplan-Neofluar 2.5x/0.06 M27 422320-9900-000
EC Epiplan-Neofluar 2.5x/0.06 HD M27 422320-9960-000
EC Epiplan-Neofluar 2.5x/0.06 HD M27 422322-9960-000
EC Epiplan-Neofluar 2.5x/0.06 M27 422322-9900-000
EC Epiplan-Neofluar 2.5x/0.06 Pol 442313-9902-000
EC Epiplan-Neofluar 2.5x/0.06 Pol M27 422323-9900-000
EC Epiplan-Neofluar 5x/0.13 DIC M27 000000-1156-511
EC Epiplan-Neofluar 5x/0.13 HD DIC M27 000000-1156-514
EC Epiplan-Neofluar 5x/0.13 Pol 442323-9901-000
EC Epiplan-Neofluar 5x/0.13 Pol M27 000000-1156-512
EC Epiplan-Neofluar 5x/0.13 HD M27 422330-9960-000
EC Epiplan-Neofluar 10x/0.25 HD M27 422340-9960-000
EC Epiplan-Neofluar 10x/0.25 DIC M27 422342-9900-000
EC Epiplan-Neofluar 10x/0.25 Pol 442333-9901-000
EC Epiplan-Neofluar 10x/0.25 Pol M27 422343-9900-000
EC Epiplan-Neofluar 10x/0.25 HD DIC M27 422342-9960-000
EC Epiplan-Neofluar 20x/0.50 HD M27 422350-9960-000
EC Epiplan-Neofluar 20x/0.5 DIC M27 000000-1156-521
EC Epiplan-Neofluar 20x/0.5 HD DIC M27 000000-1156-524
EC Epiplan-Neofluar 20x/0.5 Pol 000000-1170-667
EC Epiplan-Neofluar 20x/0.5 Pol 000000-1156-522
EC Epiplan-Neofluar 50x/0.80 HD M27 422370-9960-000
EC Epiplan-Neofluar 50x/0.8 DIC M27 000000-1156-527
EC Epiplan-Neofluar 50x/0.8 HD DIC 000000-1156-528
EC Epiplan-Neofluar 50x/0.8 Pol 000000-1170-666
EC Epiplan-Neofluar 50x/0.8 Pol 000000-1156-526
EC Epiplan-Neofluar 50x/1.0 Oil Pol 422373-9900-000
EC Epiplan-Neofluar 100x/0.90 HD M27 422390-9960-000
EC Epiplan-Neofluar 100x/0.90 DIC M27 422392-9900-000
EC Epiplan-Neofluar 100x/0.90 HD DIC 422392-9960-000
EC Epiplan-Neofluar 100x/0.90 HD DIC 442385-9902-000
EC Epiplan-Neofluar 100x/0.90 Pol 442383-9903-000
EC Epiplan-Neofluar 100x/0.90 Pol M27 422393-9900-000
LD EC Epiplan-
Neofluar
LD EC Epiplan-Neofluar 20x/0.22 DIC M27 422452-9900-000
LD EC Epiplan-Neofluar 20x/0.22 HD DIC M27 422452-9960-000
LD EC Epiplan-Neofluar 50x/0.55 DIC M27 422472-9900-000
LD EC Epiplan-Neofluar 50x/0.55 HD DIC M27 422472-9960-000
LD EC Epiplan-Neofluar 100x/0.75 DIC 000000-1156-532
LD EC Epiplan-Neofluar 100x/0.75 HD DIC M27 000000-1156-538
Table 1 Compatible objectives for use with Auto Focus

PRODUCT DESCRIPTION
Auto Focus equipment Selectable stages Carl Zeiss
06/2013 425131-7044-001 15
Objective magnification, primary capture range and precision of Auto Focus:
Objective
magnification
Max. capture range
in µm (highly
reflective plain
surface)
Maximum precision of the focus
position (exact)
(~0.3 depth of field of the
objective) in µm
Minimum size of
focused object in µm
1.25 > 12000 ~170.00 ~2000
2.5 > 10000 ~42.00 ~1000
5 > 10000 ~8.90 ~500
10 > 8000 ~2.50 ~250
20 > 4000 ~0.60 ~125
50 > 700 ~0.25 ~50
100 > 150 ~0.20 ~25
Table 2 Objective magnification, primary capture range, maximum precision and minimum object
size
The precision of the focus position of the Auto Focus varies depending on the type of objective, the
objective magnification and the optical characteristics of the sample.
The positioning accuracy and the speed of the Auto Focus vary depending on the type of objective, the
objective magnification, the optical characteristics of the sample and the work mode (see section 4.2 on
page 23) of the Auto Focus.
Appropriate HD, DIC, and pol objectives, and objectives with W 0.8 thread are also suitable.
Only EC Epiplan objectives (with magnification of 10x or less) and EC Epiplan-Neofluar objectives
(with magnification of 10x or less) on the list of objectives can be used for samples with a cover
slip.
2.5 Selectable stages
The Auto Focus equipment is compatible with all stages (manual / motorized) on the Axio Imager 2 with
motorized Z drive stand and Axio Imager Vario stand with focusing objective turret.

PRODUCT DESCRIPTION
Carl Zeiss Functionality Auto Focus equipment
16 425131-7044-001 06/2013
2.6 Functionality
The structured light generated by an LED in the sensor module of the Auto Focus is deflected through the
objective onto the sample and reflected from its surface. The Auto Focus automatically seeks the
maximum of the reflection signal, thereby bringing the surface of the sample into sharp focus.
Auto Focus can be operated in three different surface property modes (reflective / partially reflective /
diffuse) and with three different levels of precision (exact / balanced / fast). Further details can be found
in section 4.2 on page 23.
Changes in the focus position are registered by the sensor, with any deviations being automatically
compensated by the direct access of the controller to the Z drive of the microscope.
Samples can be used with or without a cover slip.
Only EC Epiplan objectives (with magnification of 10x or less) and EC Epiplan-Neofluar objectives
(with magnification of 10x or less) on the list of objectives can be used for samples with a cover
slip.
1LED
2Sensor module
3Sensor
4Beam splitter
5Objective
6Sample
Fig. 3 Function diagram of Auto Focus
Fig. 4 Auto Focus working modes
Sample
Cover slip
Standard mode of Auto Focus, focusing on the
surface of the sample
Standard mode for samples with cover slip,
focusing through the cover slip on the sample
Sample

PRODUCT DESCRIPTION
Auto Focus equipment Technical data Carl Zeiss
06/2013 425131-7044-001 17
2.7 Technical data
2.7.1 Dimensions and weight
Dimensions
Sensor module (length x width x height) ....................................................130 mm x 105 mm x 116 mm
Controller (length x width x height) ...........................................................220 mm x 250 mm x 158 mm
Weight
Sensor module ......................................................................................................................ca. 1300 g
Controller...............................................................................................................................ca. 4800 g
2.7.2 Ambient conditions
Transportation (in packed state):
Permissible ambient temperature .................................................................................. -40 °C to +70 °C
Storage
Permissible ambient temperature ................................................................................... +5 °C to +40 °C
Permissible relative air humidity (no condensation) ....................................................max. 75 % at 35 °C
Operation
Permissible ambient temperature ................................................................................. +10 °C to +40 °C
Permissible relative air humidity (no condensation) ....................................................max. 75 % at 35 °C
Max. altitude of operating site ....................................................................................................2000 m
Atmospheric pressure .............................................................................................800 hPa to 1060 hPa
Degree of pollution...............................................................................................................................2

PRODUCT DESCRIPTION
Carl Zeiss Technical data Auto Focus equipment
18 425131-7044-001 06/2013
2.7.3 Operating data
Sensor module with IR-LED
Complete device ....................................................................................................................LED class 1
Internal LED ..........................................................................IRED 850 nm / max. 950 mW / LED class 3B
conforming to DIN EN 60825-1:2003
Auto Focus....................................................................... Free group conforming to DIN EN 62471:2009
fc12 focus controller
Type of operation site.............................................................................................Closed room facilities
Protection class .................................................................................................................................... I
Ingress protection rating..................................................................................................................IP 20
Electrical safety ....................................................................................to DIN EN 61010-1 (IEC 61010-1)
conforming to CSA and UL regulations
Overvoltage category ............................................................................................................................II
Radio interference suppression ................................................................................ to EN 55011 Class A
Noise immunity ..........................................................................................................to DIN EN 61326-1
Line input voltage ................................................................................. 100 V AC to 240 V AC (± 10 %)
No line voltage conversion required!
Line frequency........................................................................................................................ 50 / 60 Hz
Power consumption ..............................................................................................................max. 80 VA
Fuses according to IEC 60127
fc12 focus controller .....................................................................................T 2.0 A/H; 250 V; 5x20 mm
Operating data for Axio Imager 2 / Axio Imager Vario, motorized with external VP232-2 power
supply pack.
Type of operating site.............................................................................................Closed room facilities
Protection class .................................................................................................................................... I
Ingress protection rating..................................................................................................................IP 20
Electrical safety ....................................................................................to DIN EN 61010-1 (IEC 61010-1)
conforming to CSA and UL directives
Overvoltage category ............................................................................................................................II
Radio interference suppression .................................................................................to EN 55011 Class B
Noise immunity ..........................................................................................................to DIN EN 61326-1
Line input voltage with external power supply VP232-2 ...................................100 V AC to 240 V ±10 %
No line voltage conversion required!
Line frequency........................................................................................................................ 50 / 60 Hz
Max. power consumption Axio Imager, motorized................................................................max. 190 VA

INSTALLATION
Auto Focus equipment Connecting Auto Focus Carl Zeiss
06/2013 425131-7044-001 19
3INSTALLATION
The Auto Focus consists of the following main assemblies
•Auto Focus equipment and controller (425131-9000-000) or successor
•Auto Focus installation set (425131-9030-000) or successor
Also required:
•Camera deflection left, manual (425103-0000-000) or successor
or
•Camera deflection left, motorized (425104-0000-000) or successor
An Axio Imager 2 / Axio Imager Vario stand with motorized Z-axis drive /
focusing objective
turret is required in order to operate Auto Focus. Retrofitting to existing older Axio Imager 2 or
Axio Imager Vario stands must be carried out by a Carl Zeiss customer service employee. Axio
Imager 2 stands without motorized Z-
axis drive and all Axio Imager Vario stands without
focusing objective turret cannot be equipped with Auto Focus.
Retrofitting of Axio Imager "1" stands is not possible.
3.1 Connecting Auto Focus
Fig. 5 Connection diagram for Auto Focus and controller to the Axio Imager 2 stand

INSTALLATION
Carl Zeiss Connecting Auto Focus Auto Focus equipment
20 425131-7044-001 06/2013
Switch off the power supply of the stand
and the PC before connecting the
controller to the microscope.
Ensure that the cable from the
Auto
Focus sensor to the controller and
from the
controller to the microscope
stand is not too tight, thereby permitting
the Z-axis drive and the focusing
objective turret to function correctly.
•Convert the standard camera deflection left
port with the Auto Focus installation set
(425131-9030-000) or successor.
•Fasten the Auto Focus to the left side camera
deflection.
•Connect the Auto Focus to the controller. To do
so, insert the Firewire cable into the connections
of the Auto Focus (Fig. 6/2) and the controller
(Fig. 7/4). Then insert the control cable into the
connections on the Auto Focus (Fig. 6/1) and
the controller (Fig. 7/3).
•Connect the controller to the microscope stand
using the CAN cable. To do so, insert the CAN
cable into the connection on the controller
(Fig. 7/2) and on the microscope.
•Connect the controller to the power supply
(Fig. 7/1).
1Control cable connection
2Signal cable connection (Firewire)
3Scale for manual adjustment
Fig. 6 Auto Focus sensor module
1Power connection
2CAN connection
3Control cable connection
4Signal cable connection (Firewire)
Fig. 7 Rear view of controller and
connections