CEM Razor User manual

Razor®
Rapid Peptide Cleavage System
Manual

THIS PAGE INTENTIONALLY LEFT BLANK

Contents
Safety
Safety Notations 1
Introduction
About the Instrument 2
Recommended Cleavage Parameters 2
Important Safeguards
Instrument Safeguards 3
Instrument Overview
Razor Hardware 4
Accessory Kit 5
System Installation
System Setup 6
Assign the Set Temperature 8
Peptide Cleavage
Prepare the Razor 9
Rinse Resin Prior to Cleavage 10
Cleavage 12
Precipitation and Analysis 12
Sample Position Cleaning 13
Maintenance
Cavity Ceiling Cleaning 14
Sample Position Clog 14
System Specifications
Location 15
Electrical Requirements 15
Environmental Constraints 15
Warranty
Limited Warranty Information 16
Technical Assistance
Applications Support 18
Technical Support 18
Contact Information
CEM Corporation Headquarters 19
CEM International Subsidiaries 19
CEM Distributors 20

THIS PAGE INTENTIONALLY LEFT BLANK

1Safety
Safety
Safety Notations
This manual uses three safety alert words at points in the documentation where the user should be aware of
potential hazards. The safety alerts are shown in color-coded boxes. The three words – NOTE, CAUTION, and
WARNING – indicate differing levels of observation or action as described below:
NOTE
A NOTE is intended to provide emphasis on procedures that may be misinterpreted or overlooked, or to
clarify confusing situations.
CAUTION
A CAUTION is intended to provide essential information and to emphasize procedures that, if not strictly
followed, may result in improper instrument operation.
WARNING
A WARNING is intended to emphasize dangerous or hazardous conditions that may result in personal
injury to the user and/or damage or destruction of the instrument.

2 Introduction
Introduction
About the Instrument
The Razor parallel cleavage system is an isolated heating module designed for performing the peptide cleavage
step. The isolated module provides the advantage of protecting the Liberty Blue™system from the harsh reagents
required for the cleavage all while allowing the use of heat to provide high quality peptides in excellent yields in a
fraction of the time.
This manual is intended for use by both novice and experienced users for the operation and maintenance of the
Razor.
Recommended Cleavage Parameters
In Fmoc synthesis, the removal of the peptide from the solid support is typically accomplished with triuoroacetic
acid (TFA). Because the side chain protecting groups used in Fmoc synthesis are acid labile, a single step both
cleaves the peptide from the resin and removes the protecting groups. Various scavenger molecules are added
to the TFA to prevent the cleaved protecting groups from reattaching to the peptide. The particular scavengers
used depend on the specic peptide sequence. Common scavengers include water (scavenges t-butyl cations),
triisopropyl silane (TIS, scavenges trityl and Pbf cations), ethane dithiol (EDT, scavenges t-butyl cations, reduces
oxidation of Cys/Met side chains), dioxa-1,8-octane-dithiol (DODT, scavenges t-butyl cations, suppresses oxidation
of Cys/Met side chains), phenol (protects Tyr and Trp side chains from oxidation), and thioanisole (aids in removal
of Pbf protecting groups from Arg(Pbf), suppresses oxidation of Cys/Met side chains).
CEM recommends using a cocktail of TFA/TIS/H2O/DODT (92.5/2.5/2.5/2.5).

3Important Safeguards
Important Safeguards
General guidelines for safe operation of the Razor system are presented below and all specic safety messages
are located throughout the manual.
Instrument Safeguards
• Service can only be performed by an authorized CEM service technician. The system must be disconnected from
power supply prior to servicing.
• Do not tamper with the Razor, including actions such as removing any components of the housing.
• The Razor must be installed in a ventilated fume hood pulling at least 30.5 CFM.
• Adequate ventilation should be provided for preparation of reagents and solvents for use on the system.
• If any damage to the instrument is noted, do not attempt instrument operation.
• Cleavage vessels must be purchased directly from CEM Corporation or through its authorized dealer network.
• If it is necessary to use an extension cord, use only a 3-wire extension cord that has a 3-blade grounding plug
and a 3-slot receptacle that will accept the plug from the instrument. The marked rating of the extension cord
must be equal to or greater than the electrical rating of the instrument.
• If instrument is used in a manner not specied by CEM Corporation in this manual, then the protection provided
by the equipment may be impaired.
• Proper precautions must be taken to avoid contact with reagents or reagent vapors. Protective gear should be
worn as outlined in the user’s safety program for hazardous materials and the reagent manufacturer’s safety
data sheet. Refer to these guidelines for proper handling and disposal of the reagents. Dispose of all waste in
accordance with all applicable local, state, and federal health and safety recommendations.
• Do not position the Razor so it is difcult to access the power, heat or vacuum switches

4 Instrument Overview
Instrument Overview
For a list of parts and part numbers, visit our website at www.cem.com. A list of parts can be found on the
“Products” page under “Razor.”
Razor Hardware
1
11
9
8
7
6
5
4
3
2
10
12
Item Item
1 Vessel Hot Block 7 Light Switch
2 Valve Panel: Sample Positions, Vent, and Drain knobs 8 Power Switch
3Vessel Tray 9 Heat Switch
4 Temperature Control Panel 10 Vacuum Switch
5 Cavity 11 Vacuum Gauge
6 Locking Lever 12 Ports: Vent, Vacuum, and Waste

5Instrument Overview
Accessory Kit
Vessel Rack Vessel Rack Cover Waste Tubing Bundle
P/N 547205 P/N 170875 P/N 547225
GL45 Waste Cap Razor Power Supply Position Cleaning Tool
P/N 167545 P/N 267080 P/N 547300
1 L Glass Bottle (no cap) Vessel Caps 50 mL Centrifuge Tubes
P/N 167550 P/N 170810 P/N 330090 (Set of 25)
Cleavage Vessel Spill Tray
P/N 170897 (Pack of 60) P/N 170530
P/N 170900 (Pack of 120)
*Power Cord not pictured as it is region specic.

6 System Installation
System Installation
System Setup
1. Remove the Razor from it’s shipping box and place the system in a well ventilated fume hood.
WARNING
The Razor must be installed in a ventilated fume hood pulling at least 30.5 CFM.
2. Connect the power cord to the power supply box. Attach the power supply to the connector on the rear of the
system, and plug the power cord into an appropriate outlet.
3. Connect the waste tubing.
3.1. Connect the tubing bundle to the appropriate ports on the side of the system: vacuum and waste.
3.2. Attach the opposite end of the tubing bundle to the GL45 cap. Both ports on the cap are the same so
tube placement is indifferent.