WPI LWCC-M-10 User manual

LWCC-M-10
Low Volume Flow Cell
INSTRUCTION MANUAL
Serial No._____________________
102809
LWCC-M-10
Warranty
WPI (World Precision Instruments, Inc.) warrants to the original purchaser that this equipment, including its
components and parts, shall be free from defects in material and workmanship for a period of 30 days from the
date of receipt. WPI’s obligation under this warranty shall be limited to repair or replacement, at WPI’s option, of
the equipment or defective components or parts upon receipt thereof f.o.b. WPI, Sarasota, Florida U.S.A. Return of a
repaired instrument shall be f.o.b. Sarasota.
The above warranty is contingent upon normal usage and does not cover products which have been modified
without WPI’s approval or which have been subjected to unusual physical or electrical stress or on which the
original identification marks have been removed or altered. The above warranty will not apply if adjustment, repair
or parts replacement is required because of accident, neglect, misuse, failure of electric power, air conditioning,
humidity control, or causes other than normal and ordinary usage.
To the extent that any of its equipment is furnished by a manufacturer other than WPI, the foregoing warranty
shall be applicable only to the extent of the warranty furnished by such other manufacturer. This warranty will not
apply to appearance terms, such as knobs, handles, dials or the like.
WPI makes no warranty of any kind, express or implied or statutory, including without limitation any warranties
of merchantability and/or fitness for a particular purpose. WPI shall not be liable for any damages, whether direct,
indirect, special or consequential arising from a failure of this product to operate in the manner desired by the
user. WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use
of this product.
Claims and Returns
• Inspect all shipments upon receipt. Missing cartons or obvious damage to cartons should be noted on the
delivery receipt before signing. Concealed loss or damage should be reported at once to the carrier and an
inspection requested. All claims for shortage or damage must be made within 10 days after receipt of shipment.
Claims for lost shipments must be made within 30 days of invoice or other notification of shipment.
Please save damaged or pilfered cartons until claim settles. In some instances, photographic documentation may
be required. Some items are time sensitive; WPI assumes no extended warranty or any liability for use beyond the
date specified on the container.
• WPI cannot be held responsible for items damaged in shipment en route to us. Please enclose merchandise in its
original shipping container to avoid damage from handling. We recommend that you insure merchandise when
shipping. The customer is responsible for paying shipping expenses including adequate insurance on all items
returned.
• Do not return any goods to WPI without obtaining prior approval and instructions (RMA#) from our returns
department. Goods returned unauthorized or by collect freight may be refused. The RMA# must be clearly
displayed on the outside of the box, or the package will not be accepted. Please contact the RMA department for
a request form.
• Goods returned for repair must be reasonably clean and free of hazardous materials.
• A handling fee is charged for goods returned for exchange or credit. This fee may add up to 25% of the sale
price depending on the condition of the item. Goods ordered in error are also subject to the handling fee.
• Equipment which was built as a special order cannot be returned.
• Always refer to the RMA# when contacting WPI to obtain a status of your returned item.
• For any other issues regarding a claim or return, please contact the RMA department
Warning: This equipment is not designed or intended for use on humans.
World Precision Instruments, Inc.
USA: International Trade Center, 175 Sarasota Center Boulevard, Sarasota FL 34240-9258 USA
Tel: [phone] • Fax: [phone] • E-mail: [e-mail] • Internet: http://www.wpiinc.com
Germany: Zossener Str. 55, 10961 Berlin, Germany • Tel: [phone] • Fax: [phone] • E-mail: [e-mail]
UK: Astonbury Farm Business Centre • Aston, Stevenage, Hertfordshire SG2 7EG England • Tel: [phone] • E-mail: [e-mail]
China & Hong Kong: WPI Shanghai Trading Co., Ltd. • Tel: [phone] • E-mail: [e-mail]
www.wpiinc.com

LWCC-M-10
MicroLWCC (WPI #LWCC-M-10) is a new fiber optic, low volume flow cell for UV/
VIS/NIR absorbance analysis.
Based on WPI’s established
liquid core waveguide tech-
nology, the analyte solution
functions as the core of a fluid
filled light waveguide. Wetted
parts in the sample cell light
path are PEEK, fused silica and
Teflon. Optical fibers are used
to transport light to and from
the sample cell. The cell can be
used in biochemistry for DNA,
RNA and protein quantification,
colorimetric nutrient and trace
metal analysis, drug discovery
and dissolution testing, process
control and HPLC analysis.
ASSEMBLY
Light source and detector can be connected to the LWCC-M-10 via two SMA-termi-
nated fiber optic cables with a core diameter of 600µm. The fiber optic connections
are interchangeable in that either connector can be used to connect to the light
source or to the spectrometer.
As with the fiber optic connectors, the liquid ports are also interchangeable. It makes
no difference which port is set-up as inflow and which port serves as the outflow.
However, if an in-line filter is to be installed, it should be connected on the input side
of the liquid flow path. Use standard HPLC type 10-32 coned PEEK 1/16th tubing fit-
tings (WPI #800827) to make connections to the pump and waste receptacle.
OPERATING INSTRUCTIONS
The following procedures can be used to verify the transmission, noise and refractive
index of your LWCC-M.
Transmission Verification
1. Connect one 600mm diameter fiber optic assembly to a light source and one
600mm diameter fiber optic assembly to the detector.
2. Use an SMA bulkhead adapter (WPI #13395) to butt-couple the fibers. This is the
reference for the measurement of transmission.
3. Remove the SMA bulkhead adapter and insert the LWCC-M.
4. Record the percent transmission. Values for the 254nm, 280nm and 550nm
should exceed 40%.
Noise Determination
Noise is determined using the ASTME685-93 standard as reapproved in 2000. Use
methanol as the solvent at a flow rate of 1.0mL/min. Using the WPI test setup, noise
values are below 35uAU. See www.astm.org/Standards/E685.htm to obtain a copy of
the standards document.
Refractive Index
The refractive index between methanol and cyclohexane is measured using a flow
rate of 3.0mL/min. Because of the immiscibility of the solvents, ethanol is used be-
tween the solvents to prevent unwanted interaction between methanol and clyclohex-
ane. An RI value between zero and seven is acceptable at the 280nm wavelength.
MAINTENANCE
Cleaning
With normal use, the LWCC-M-10 will rarely need cleaning. If measurement results
diminish, clean your LWCC-M-10. Use the cleaning kit for liquid waveguides (WPI
#501609).
1. Connect the exit tubing (for example, silicon or equivalent) from the fluid output
of the LWCC-M-10 to a waste container.
2. Rinse the cell thoroughly using Ultra Pure water. Obtain a new reference intensity
and take a baseline absorbance reading.
3. Inject 1cc “Cleaning Solution 1” into the LWCC-M-10 fluid input port.
4. Inject 1cc “Methanol Solution 2” into the fluid input port of the LWCC-M-10.
5. Inject 1cc “HCl Solution 3” into the fluid input port of the LWCC-M-10.
6. Flush out the cleaning solutions with distilled, Ultra Pure, reverse osmosis or
equivalent quality water and take an absorbance reading.
7. Repeat these cleaning cycles until a stable absorbance signal can be obtained.
8. If the UV readings are still low after cleaning, swab the fiber optic surfaces of the
sample cell with a fiber optic Q-tip dipped in isopropyl alcohol.
Storage
To store the instrument, clean the LWCC-M-10, and then seal the fluid input and
output ports using either the caps provided or an alternative.
SPECIFICATIONS
LWCC-M-10
Optical pathlength 10mm
Internal volume 2.4mL
Wavelength Range 200–1000nm
Fiber Connection (SMA) 600µm
Transmission at 254nm* > 40%
Liquid Connection 10-32 coned port for 1/16th tubing
Maximum Pressure 1000 PSI
Refractive Index at 280nm** < 7 mAU
Wetted materials PEEK, Fused Silica, Teflon
* Reference: Two 600µm fibers, butt-coupled
** Measured using ASTM E 685 - 93
WPI U.S. Patents: 5,444,807; 5,570,447; 5,604,587; 6,603,556; 6,385,380.
Light Input
600µm SMA Light Output
600µm SMA
Fluid Input
800827 Connector
Fluid Output
800827 Connector
1.00"
1.38”
Fig. 1–LWCC-M-10 showing connections.