Cole Parmer 74900-20 User manual

Dual & Multiple
Syringe Infusion Pump
Users Manual
Publication 5607-012-REV-

EU Directives WEEE and RoHS
To Our Valued Customers:
We are committed to being a good corporate citizen. As part of that commitment,
we strive to maintain an environmentally conscious manufacturing operation. The
European Union (EU) has enacted two Directives, the first on product recycling
(Waste Electrical and Electronic Equipment, WEEE) and the second limiting the use
of certain substances (Restriction on the use of Hazardous Substances, RoHS).
Over time, these Directives will be implemented in the national laws of each EU
Member State.
Once the final national regulations have been put into place, recycling will be offered
for our products which are within the scope of the WEEE Directive. Products falling
under the scope of the WEEE Directive available for sale after August 13, 2005 will
be identified with a “wheelie bin” symbol.
Two Categories of products covered by the WEEE Directive are currently exempt
from the RoHS Directive – Category 8, medical devices (with the exception of
implanted or infected products) and Category 9, monitoring and control instruments.
Most of our products fall into either Category 8 or 9 and are currently exempt from
the RoHS Directive. We will continue to monitor the application of the RoHS
Directive to its products and will comply with any changes as they apply.
• Do Not Dispose Product with Municipal Waste
• Special Collection/Disposal Required
WEEE/RoHS Compliance Statement

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Cole- armer®Dual & Multiple Syringe ump
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Contents
Specifications..........................................................................................................................3
General Safety Summary......................................................................................................4
General Description ..............................................................................................................5
Features ................................................................................................................................5-7
Operating Instructions
Power Switch ............................................................................................................8
Syringe Loading........................................................................................................8
Withdrawal Operation ............................................................................................8
Push/Pull Syringe Pump ..........................................................................................9
Menu Features ........................................................................................................10
Keypad Functions....................................................................................................10
Fast Forward, Fast Reverse ....................................................................................10
Syringe Diameter Entry ..........................................................................................11
Volume Setting ........................................................................................................11
Units Setting ............................................................................................................12
Rate Setting..............................................................................................................12
Power Interruption (power up) ..............................................................................12
Run/Stop ................................................................................................................12
Change or Review Volume Setting While Running ............................................13
Mode Selection ......................................................................................................13
Infusion
Withdrawal
Infusion/Withdrawal
Withdrawal/Infusion
Continuous
Manual Stall Setting and Microliter Syringes ....................................................14
Glass Syringes ........................................................................................................14
Clearing a Stall Condition ....................................................................................15
NV Ram Failure ......................................................................................................15
RS232 Connections................................................................................................16
RS232C Commands ........................................................................................16-19
RS232 Format ........................................................................................................19
Programmable Option
General Information ..............................................................................................20
Features....................................................................................................................21
Menu Operation ..............................................................................................22-24
Running the Program..............................................................................................25
Commands and Responses ............................................................................26-29
RS232 Commands ..............................................................................................................30
TTL Specifications ................................................................................................................31

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Fuses ......................................................................................................................................31
Maintenance ........................................................................................................................32
Standard Table of Syringe Diameters ..............................................................................33
Japanese Table of Syringe Diameters ..............................................................................34
Standard Minimum and Maximum Flowrates..................................................................35
Japanese Minimum and Maximum Flowrates ................................................................36
Limited Warranty..................................................................................................................37
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Specifications
Model Dual Infusion Pump
Syringe size 10 microliter to 140 milliliter
Electrical Rating US model 115 V~, 0.25 A
CE model 230 V~, 0.16 A
Fuse 5 x 20 mm, 250 V~, Slow low, 0.25 A
Voltage operating range US model 110-120 V~, 50/60 Hz
CE model 200-240 V~, 50/60 Hz
Drive mechanism Microprocessor controlled stepper motor
1/2 - 1/16 microstepping, driving a leadscrew
through a belt and pulley drive mechanism
Pusher advance per microstep (1/16 step) - 0.165 micron (or .0000064 inch)
Volume per microstep (1/16) with 60ml D syringe - 0.0919µl
Max stepping rate 1600 (1/2 step) /sec
Min step rate 1 (1/16 step) /120 secs
Linear Travel Rate Min: 4.95 x10-4 cm/hr
Max: 12.67 cm/min
Flowrate range 2.757 µl/hr to 70.56 ml/min (60 ml syringe)
Nominal linear force 40 lb
Dimensions 11x9x5.5 inch (28x23.5x14 cm)
Weight 9 lb (4 kilo)
Atmospheric Specifications
Temperature 5˚C - 40˚C (41˚F - 104˚F)
Humidity 20% - 80% RH
Mode of Operation Continuous
Classification Class I
Pollution Degree 2
Installation Category II
Output N/A
Physiological Effects N/A
Cooling Conditions No special considerations
Mechanical Stability No special considerations
Protective Packaging No special considerations
Earth Terminals No External connections required
Removable Protective Means N/A
Supplier Name Cole-Parmer®Instrument Company
Address 625 East unker Court
Vernon Hills, Illinoius 60061-1844
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General Safety Summary
Please read the following safety precautions to ensure proper use of your syringe pump.
To avoid potential hazards and product damage, use this product only as instructed in this
manual. If the equipment is used in a manner not specified by the manufacturer, the protection
provided by the equipment may be impaired.
To revent Hazard or Injury:
USE PROPER POWER SUPPLY
The pump is supplied with an approved power supply and line cord.
USE PROPER LINE CORD
Use only the line cord shipped with the product and make sure line cord is certified for coun-
try of use.
GROUND PRODUCT
This product is grounded through the grounding conductor of the power cord. To avoid electric
shock, use only approved line cord with the product and insure it is connected to earth ground.
MAKE PROPER CONNECTIONS
Make sure all conections are made properly and securely.
ORIENT EQUIPMENT PROPERLY
Do not position the equipment such that it is difficult to reach the disconnecting device.
OBSERVE ALL TERMINAL RATINGS
Review the operating manual to learn the ratings on all connections.
AVOID EXPOSED CIRCUITRY
Do not touch any electric circuitry inside the product.
DO NOT OPERATE WIT SUSPECTED FAILURES
If damage is suspected on or to the product do not operate the product. Contact qualified
service personnel to perform inspection.
AVOID PINC AZARD
A pinch hazard may exist between the Pusher lock and End locks. Avoid placing fingers
between these points while the pump is running.
OBSERVE ALL WARNING LABELS ON PRODUCT
Read all labels on product to ensure proper usage.
CAUTION
This pump is not registered with the FDA and is not for clinical use
on human or veterinary patients. It is intended for research use only.
Protective Ground
Terminal
CAUTION
Refer to Manual
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General Description
Operation of the pumps is simplified by using a keypad to select features from a menu on the
alphanumeric display.
All control functions are performed automatically by the pump microcontroller and are based
on the syringe diameter and linear motion of the pusher block to deliver a known volume.
After entering the syringe diameter, either directly or from a table in memory, a dispense
volume and flow rate can be entered, and then all calibration and control functions are
performed by the pump automatically.
model number syringes infusion withdrawal push/pull
74900-10 2x
74900-20 2xx
74900-30 10 x
74900-40 10 xx
74900-70 4x
74902-60 4x
74901-50 4x
Features
1) Syringe identification
Look up Table
The pump contains a table of standard syringes arranged by manufacturer, material
and size. Once the syringe is identified in the table the pump automatically enters the
appropriate diameter.
Direct Entry
If the syringe used is not included in the table, the internal diameter of the syringe
barrel can be measured in millimeters and entered directly from the keypad.
2) INFUSION AND REFILL RATES
The infusion rate and, where applicable, the withdrawal rate can be set independently
and can be changed while the pump is running. After the operating mode selection is
made the program will prompt only for the relevant rates associated with that mode.
3) VOLUME
A target volume can be entered for infusion and refill independently, and the pump
automatically stops when this volume is reached. The pump displays an initial volume of
zero and increases as the dispense proceeds to the target volume. The target volume
can be reviewed or changed as the pump continues to operate.
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Features (continued)
4) MODES OF OPERATION
Infusion
Rate and volume settings: pump infuses to the set volume and stops.
Rate setting only: pump runs until manually stopped or stalls.
Withdrawal
Rate and volume settings similar to above.
Infusion/withdrawal
Infusion automatically followed by withdrawal. Rate and volume settings can be made
independently for infusion and withdrawal, hence the pump can infuse at one rate and
volume and then change to a different withdrawal flowrate and volume setting.
Withdrawal/infusion
Withdrawal immediately followed by infusion. Separate settings for rate and volume
can be made for withdrawal and infusion.
Continuous operation
The pump cycles from infusion to withdrawal continuously. The volume is identical in
infuse and withdrawal directions.
Note: The displayed menu which prompts the operator for Rate and Volume settings
changes with Mode selection. For convenience, only the relevant settings associated
with the selected mode are prompted. For example, in the Withdrawal / Infusion mode
the menu prompts for withdraw and then infuse volumes, followed by withdraw and
infuse rates. In Infusion only mode, the menu prompts only for infusion volume and infu-
sion rate. In the Continuous mode only one volume is prompted for followed by infusion
and withdrawal flow rates.
5) RS232C INTERFACE
Multiple pumps can be controlled in a “daisy chain” by a single PC.
Programming is reduced to a small number of simple commands.
6) TTL
Input and output controls are available, such as, direction change, run indicator,
footswitch or timer control , and valve or relay actuation.
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Features (continued)
7) STALL DETECTION
The motor is monitored by an optical encoder to confirm the programmed movement. If
the back pressure increases due to jamming or flow restriction then the motor may stall.
Stall detection by the encoder results in a pump shutdown.
The display will read “Stalled”. The Stall message can be cleared with the Select key.
8) POWER DISRUPTION
When power is returned after a temporary power disruption the pump can be pro-
grammed to resume operation or remain stopped.
However, if a dispense volume is set then the pump always remains stopped.
9) NON-VOLATILE MEMORY
All operational settings are stored in non-volatile memory for convenience, and are
used to set the pump when first switched on.
10) SELECTION OF RATE AND VOLUME UNITS
Units of volume (µl or ml) and flowrate (µl/ml per min/hr) can be changed if required.
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To simplify syringe loading, the pusher block can be disengaged from the leadscrew by turn-
ing the knob(1) and manually moved along the guide rods. Alternately, the Fast forward,
Fast reverse feature can be used (press Run and respective Arrow key simultaneously).
Knob (1) is rotated to release the drive nut from the leadscrew. When the white line on the
knob is facing toward the syringe barrel the drive nut is engaged and when the white line
faces the keypad the nut is disengaged.
Raise and rotate the spring loaded retaining arm (2) and place the syringe barrel in the “V”
of the syringe holder. With the syringe in place release the retaining arm so that it clamps
down on the barrel and holds it securely in place.
Release the pusher block mechanism and slide the block along the guide rods until the block
presses firmly against the syringe plunger. Rotate the knob (1) to re-engage the drive nut.
MODEL 210 AND MODEL 230 WIT DRAWAL OPERATION
For withdrawal, or refill operation, the syringe plunger and barrel flange must be secured by
additional brackets.
Loosen screws (3) on the syringe holder and, after loading the syringe, press the retaining
bracket against the barrel flange to secure the flange between the bracket and syringe
holder. Tighten screws (3).
To restrain the plunger, rotate knob (4) to release the retaining bracket and position the
syringe plunger between the pusher block and retaining bracket. Turn knob (4) clockwise to
tighten the retaining bracket and then re-engage the drive nut knob (1).
The mechanism is designed to accommodate a wide range of syringe sizes however, there
are some syringes which require additional attention. (See page 14)
Operating Instructions
POWER SWITC
The power switch is located on the right corner of the rear panel.
SYRINGE LOADING
(4) Anti-syphon knob (1) Drive nut knob
Anti-syphon bracket (3) Withdraw screw
(2) Syrin e clamp
Cole- armer®Dual & Multiple Syringe ump