CORNING LSE 6765-HS User manual

INSTRUCTION MANUAL
Models:
6765-HS -- 120V US
6766-HS -- 230V EU
6767-HS -- 230V UK
6768-HS -- 100V JP
CLS-EQ-102 March 2010, Document 01 , Revision 01

Warnings
The following precautions should be taken when operating or working near the
CorningLSEHigh Speed Microcentrifuge:
NEVER use the centrifuge in any manner not specified in these
instructions.
NEVER operate the centrifuge without a rotor properly attached to the
shaft.
NEVER tighten rotor nut by hand only.
NEVER fill tubes while they are in the rotor. Liquid spillage may harm
unit.
NEVER put hands in the rotor area unless the rotor is completely stopped.
NEVER move the centrifuge while the rotor is spinning.
NEVER use solvents or flammables near this or other electrical
equipment.
NEVER centrifuge flammable, explosive or corrosive materials.
NEVER centrifuge hazardous materials outside of a hood or proper
containment facility.
ALWAYS load the rotor symmetrically. Each tube should be counter
balanced by another tube of the same type and weight.
ALWAYS locate the centrifuge within easy access to an electrical outlet.
ALWAYS use only microcentrifuge tubes made from plastic and designed to
withstand centrifugal forces of at least 16,500 xg.
ALWAYS use a wrench to tighten the rotor nut.

Contents
GeneralInformation 1
Description
Safety precautions
Technical data
Accessories supplied with unit
Warranty
Installation 2
Unpacking the centrifuge
Required space
Installing the centrifuge
Installing the Rotor 3
Rotors and accessories
Rotor maintenance
Removing and installing the angle rotor
Loading the rotor
Overloading rotors
Operation 5
Attaching rotor lid
Closing the lid
Lid release
Lid lock
Speed selection (RPM/ g force)
Selection of operating time, momentary operation, Start/Stop
ServiceandMaintenance 7
Service
Cleaning
Disinfection
Replacing fuses
Troubleshooting 8
WheretoCall 9
Determination of g-values 9
Warranty Statement 10
Declaration of Conformity Statement 11
Equipment Disposal 12

1
1. General Information
This manual provides important safety information for the CorningLSEHigh Speed
Microcentrifuge. It should be kept near the centrifuge for quick and easy reference.
1.1 Description
The Corning LSE High Speed Microcentrifuge is a small benchtop centrifuge
designed for separation of various research samples. The motor is brushless and
requires no routine maintenance. The Corning LSE High Speed Microcentrifuge is
supplied with a 24 x 1.5/2.0 mL rotor for micro samples. Adapters are available for
tubes smaller than 1.5 mL. The Corning LSE High Speed Microcentrifuge reaches
speeds of up to 13,300 rpm/16,300 x g.
1.2 Safety precautions
Note: All users of the centrifuge must read the Safety Precautions section of
this manual before attempting to operate the unit!
If this equipment is used in a manner not specified by the manufacturer, the
protection provided by the equipment may be impaired.
DO NOT OPERATE the centrifuge IF any of the following conditions exist:
The centrifuge has not been installed properly
The centrifuge is partially dismantled
Service has been attempted by unauthorized or unqualified personnel
The rotor has not been installed securely on the motor shaft
Rotors and accessories not belonging to the standard range are being used
without permission being obtained from the manufacturer to use such rotors
and/or accessories in the centrifuge
Exception: Microcentrifuge tubes made of plastic, normally available in the
laboratory
The centrifuge is located in an explosive atmosphere
Materials to be centrifuged are combustible and/or explosive
Materials to be centrifuged are chemically reactive
The rotor load is not properly balanced
The rotor nut was not tightened with a wrench

2
1.3 Technical data
Dimensions
Width 9.25 inches (23.5 cm)
Depth 11.5 inches (29.2 cm)
Height 8.5 inches (21.6 cm)
Maximum speed 13,300 rpm
Maximum RCF 16,300 x g
Maximum volume 24 x 1.5/2.0 mL
Admissible sample density 1.2 kg/dm3
Electrical/fuse rating 120V~, 50-60Hz, 1.9A, 2.5 A
230V~, 50-60Hz, 1.1A, 1.25 A
100V~, 50-60Hz, 2.0A, 2.5A
Operating temp./humidity 0ºC to 40ºC / 80%RH
1.4 Accessories supplied with centrifuge
Each unit is supplied with 1 instruction manual, 1 warranty card, 1 power cord, a
standard rotor and a rotor removal tool.
1.5 Warranty
This centrifuge has been subjected to thorough testing and quality control. In the
unlikely event of a manufacturing fault, our one year warranty (from the date
of delivery) covers the centrifuge and the rotor. This warranty becomes invalid in
the case of incorrect operation, use of nonstandard spare parts or accessories
and unauthorized modification of the rotor or centrifuge.
Corningreserves the right to make technical modifications. Please see the
complete limited warranty statement on page 12
2. Installation
2.1 Unpacking the centrifuge
Before unpacking the centrifuge, inspect the outside of the carton for any shipping
damage.
The centrifuge is delivered in a carton with protective foam cushioning. Remove
the centrifuge from the carton. Retain the carton and cushioning until it has been
established that the centrifuge is working properly.
Inspect the centrifuge for any visible signs of shipping damage. Shipping damage
is the responsibility of the transportation carrier. Any claims for damage must be
filed within 48 hours with the carrier that delivered the centrifuge.
The accessories supplied with the centrifuge should be kept with the instruction
manual near the centrifuge’s place of installation.

3
2.2 Required space
The centrifuge should be installed on a rigid, even surface such as a stable laboratory
bench, countertop, etc. To guarantee sufficient ventilation, ensure that the centrifuge
has at least 15 cm (6 inches) of free space on all sides, including the rear.
The centrifuge should not be located in areas subject to excessive heat such as in
direct sunlight or near radiators or the exhaust of a compressor, as a buildup of heat
may occur within the chamber.
2.3 Installation
Before operating the centrifuge, check that the power source (electrical outlet on
the wall) corresponds to that on the manufacturer’s rating label, then connect the
power cord to the centrifuge and the power source.
3. Installation of rotors and rotor maintenance
3.1 Rotors and accessories
The following accessories are included or available for the CorningLSE
High Speed Microcentrifuge:
Included:
Angle rotor for 24 x 1.5 mL tubes
Tube measurement 1.5 mL / 2.0 mL (10 x 40 mm)
Max. speed 13,300 rpm
Centrifuging radius
Max/ RCF (g-value) 8.23 cm 16,276 x g
Rotor Wrench
Rotor Lid
Rotor Securing Screw
Available by contacting Corning Customer Service:
Adapter for 0.5 mL tubes (pk/6) Adapter for 0.4 mL tubes
(pk/6)
Tube measurement 8 x 30 mm Tube measurement 6 x 47 mm
Max. speed 13,300 rpm Max. speed 13,300rpm
Centrifuging radius 7.53 cm Centrifuging radius 8.23 cm
RCF (g-value) 14,892 x g RCF (g-value) 16,276 x g
Adapter for 0.2ml tubes (pk/6)
Tube measurement 6 x 21mm
Max. speed 13,300 rpm
Centrifuging radius 7.03 cm
RCF (g-value) 13,903 x g

4
3.2 Rotor maintenance
The rotor should be cleaned thoroughly after each use. Thorough cleaning must be
performed when spinning samples containing phenol or phenol chloroform.
Periodically inspect the rotor for dents, dings, scratches, discoloration and cracks. If any
damage to the rotor is found, discontinue use of the rotor immediately and replace.
3.3 Removing and installing the angle rotor
The CorningLSEHigh Speed Microcentrifuge comes complete with a standard 24
place rotor installed. To remove the rotor for cleaning, remove the rotor securing
screw from the motor shaft by turning the screw counterclockwise, using the rotor
wrench. Lift the rotor directly upward in a straight vertical motion.
To replace rotor, first make sure the motor shaft and rotor mounting hole are clean.
Place the rotor on the motor shaft. Reinstall the rotor securing screw on the motor
shaft by turning it clockwise. Hold the rotor with one hand and tighten the rotor
securing screw, using the rotor wrench.
Figure 1. Loading the rotor to achieve balance
3.4 Loading the rotor
Tubes to be loaded should be filled equally by eye. The difference in the weight
between the tubes should not exceed 0.1 gram. Tubes should always be loaded so that
there is equal spacing between all tubes. One or two additional loaded tubes may need
to be added to achieve this. Refer to Figure 1. to see one typical balancing scheme.

5
3.5 Overloading rotors
The maximum load of the rotor and the maximum speed have been established by the
manufacturer. Do not attempt to exceed these values. The maximum speed of the rotor
has been established for liquids having a homogeneous density of 1.2 g/ml or less. It is
necessary to reduce the speed in order to centrifuge liquids with a higher density.
Failure to reduce the speed may result in damage to the rotor and centrifuge. The
revised maximum speed can be calculated with the following formula:
Reduced speed (nred) = x max speed (n max)
Example:
Where the density of the liquid is 1.7, the new maximum speed would be calculated as
follows:
nred = x 13,300 = 11,174 rpm
If in doubt concerning maximum speeds, please contact the manufacturer for
assistance.
4. Operation
ATTENTION: Never attempt to operate the centrifuge with rotors or adapters
that show signs of corrosion or mechanical damage. Never centrifuge
strongly corrosive materials that may damage the rotors, accessories, or bowl
of the unit.
4.1 Attaching rotor lid
After the rotor has been properly secured and loaded, attach the rotor lid to
the rotor. Always use the rotor lid for safety and to allow the rotor to reach
proper speed. Make sure that the rotor lid snaps securely into place, by
pressing down on center catch.
4.2 Closing the lid
Close the centrifuge lid. The CorningLSEMicrocentrifuge has a lid lock that
activates only when a run is started.
4.3 Lid release
The lid will remain locked during a centrifuge run. Once the run has been
completed and the rotor has come to a stop, a beep will indicate the end
of a run, and the lid will unlock automatically.

6
WARNING: Do not attempt to open the lid of any centrifuge until the rotor has
come to a complete stop.
In the event of a power failure or malfunction, it may be necessary to open the
lid manually.
1. Disconnect the power cord from the wall socket.
2. Remove the plastic plug, located on the left side of the unit.
3. Pull the wire (attached to the plug) to open the lid lock manually.
4.4 Lid lock
The centrifuge can be started only with the lid securely closed. When a run
is started, the lid lock automatically activates. Do not attempt to open the lid
during a centrifuge run. At the end of the run, the lid will automatically unlock.
Never attempt to override the lid lock mechanism. Doing so is dangerous
and could damage the centrifuge.
4.5 Speed selection (see Figure 2.)
The speed (rpm or g-force) can be selected from 500 to 13,300 rpm in 100
rpm increments or from 100 to 16,300 x g with the knob on the right. “g-force”
or “rpm” display is set by pressing the knob. Turn the knob to increase or
decrease the value.
4.6 Selection of operating time, momentary operation, Start/Stop
Operating time can be selected from 0.5 minutes to 30 minutes by turning the
timer knob on the left. The time can be set in 0.5 minute increments from 0 to
10 minutes and in 1 minute increments from 10 to 30 minutes. After 30
minutes, the display shows “--” which indicates continuous run. In this mode,
the centrifuge will run until manually stopped. To start a run, press the timer
knob.
When the preselected time expires, the centrifuge will stop automatically.
To stop the centrifuge prior to the expiration of set time, press the timer
knob.
The centrifuge may be operated for a short run by pressing and holding the
timer knob. The centrifuge will continue to run as long as the timer knob is
depressed and the time, in seconds will count up on the time display.

7
Figure 2. CorningLSEHigh Speed Microcentrifuge control panel layout
5. Service and Maintenance
5.1 Centrifuge service
The brushless motor in the CorningLSEHigh Speed Microcentrifuge
requires no routine maintenance. Any required service should be performed by
authorized, qualified personnel only. Repairs performed by unauthorized
personnel may void the warranty.
5.2 Cleaning the centrifuge
Always keep the centrifuge housing, rotor chamber, rotor and rotor accessories
clean. All parts should be wiped down periodically with a soft cloth. For more
thorough cleaning, use a neutral cleaning agent (pH between 6 and 8) applied
with a soft cloth. Excessive amounts of liquid should be avoided. Liquid should
not come into contact with the motor. After cleaning, ensure that all parts are
dried thoroughly by hand or in a warm air cabinet (maximum temperature 50ºC).
5.3 Cleaning the rotor
The rotor should be cleaned after each use. When spinning samples containing
phenol or phenol chloroform, the rotor should be cleaned immediately after use.
5.4 Disinfection
Should a spill of infectious materials occur within the rotor or chamber, the unit
should be disinfected. This should be performed by qualified personnel with
proper protective equipment.
5.5 Replacing fuses
Check the fuse when it is recommended in the Troubleshooting Guide located
in this manual. The fuse holder is located in the power inlet on the rear of the
unit. Disconnect the power cord from the power inlet. Open the fuse holder
drawer by inserting a small screwdriver under the tab and prying it open.
Remove the innermost (operative) fuse from its retaining tabs and replace the
fuse if necessary. A spare fuse is located in the outermost chamber of the fuse
drawer. Replace only with a fuse of exactly the same value as the original.
(Fuse type may be found in the Technical data section of this manual.)