CORNING Lambda EliteMax User manual

Corning®Lambda™EliteMax
Semi-automated Benchtop
Pipettor
Instruction Manual
Catalog Number:
6070

Table of Contents
1.0 Introduction ..........................................1
1.1 Overview ..........................................1
1.2 Symbols and Conventions ..........................1
1.3 Safety Information.................................1
1.4 Instrument Identification ..........................2
1.5 Deck Positions and Orientation Convention .........3
1.6 Package Contents ..................................4
1.7 Specifications......................................5
1.8 Initial Installation..................................6
2.0 Instrument Operation .................................6
2.1 Touch Screen User Interface ........................6
2.1.1 Main Menu ..................................6
2.1.2 Numeric and Alphanumeric Keypad ...........7
2.1.3 Protocol Toolbar Description ..................8
2.1.4 Log In User Information.......................9
2.1.5 My Files User Menu...........................9
2.2 Creating and Running User Protocols ..............10
2.2.1 Plate Filling..................................10
2.2.2 Serial Dilution ...............................13
2.2.3 Plate-to-Plate Transfers ......................17
2.2.5 Linking Simple Protocols .....................26
2.2.6 Collision Detection ..........................27
3.0 Settings: General Configurations ......................28
3.1 Screen Layout and Overview.......................28
3.2 Rack Positions Menu ..............................30
3.2.1 Procedure to Align New Plates or to Check
Current Plate Positions ......................33
3.3 Tips Calibration ...................................34
3.4 Motor Settings ...................................36
3.5 System Information Tab ...........................36
3.6 User Management ................................37
3.7 Miscellaneous Settings............................38
3.8 Software Update..................................40
4.0 Spare Parts and Accessories ...........................41
5.0 Limited Warranty.....................................41
6.0 Equipment Disposal ..................................41

Instruction Manual | 1
1.0 Introduction
1.1 Overview
Thank you for purchasing the Corning® Lambda™ EliteMax Benchtop Pipettor, an affordable and easy-to-use liquid handler. From
simple plate fillings to complex protocols, the instrument is the perfect addition to your lab.
The Corning Lambda EliteMax Benchtop Pipettor can perform a variety of liquid handling tasks including:
Plate fillings from reagents reservoirs
Serial dilutions, plate-to-plate transfers, and plate replication
Well-to-well transfers (cherry picking protocol for single-channel head only)
Accurately and precisely transferring liquids and volumes between microplates
Transferring by row or by column
More complex applications can be performed such as sample preparation, timed reaction, PCR preparation, cell-based assays set up,
tube-to-tube transfers, and more
Please read the manual before operating the instrument.
1.2 Symbols and Conventions
CAUTION: This symbol refers you to important operating and maintenance (servicing) instructions within the product
Instruction Manual. Failure to heed this information may present a risk of damage or injury to persons or equipment.
Tip This symbol provides useful information to the user while using or operating the instrument.
At any time while the instrument is running, you can press the Stop button located at the bottom left of the Runtime Screen.
This will pause the instrument and give you the option to abort or to continue the protocol.
1.3 Safety Information
During the operation, maintenance, or repair of the Corning Lambda EliteMax Benchtop Pipettor, the following safety measures should
be taken or, in consequence, the safeguards provided by the Corning Lambda EliteMax Benchtop Pipettor are likely to be damaged, the
rated safety level to be reduced, and the rated operation conditions to be affected. Corning shall not be in any way responsible for the
consequences resulting from the operator not observing the following requirements.
Grounding
AC power’s grounding should be reliable to safeguard against an electric shock. The 3-pin plug supplied with the Corning
Lambda EliteMax Benchtop Pipettor power cable is a safety device that should be matched with a suitable grounded socket.
Never allow the third ground pin to be floating. If the 3-pin plug cannot be inserted, it is recommended to ask an electrician to
install an appropriate power socket.
Keeping Away from Electric Circuits
The operator should not open the Corning Lambda EliteMax Benchtop Pipettor without first consulting the Customer Service
team. Changing components or adjusting certain parameters inside the device must be performed by certified professional
maintenance personnel only. Do not change any elements while the power is still on.
AC Power Considerations
Before turning on the power, always make sure that the main voltage is within the range of required power supply and that the
rating current of the power socket meets the required specification.
AC External Power Supply Considerations
As an accessory of the Corning Lambda EliteMax Benchtop Pipettor, the external AC power supply should be placed in a safe
area. If it is damaged, the AC power supply cannot be repaired, but instead must be replaced by a new one. The external power
supply should be free of any objects and placed in a ventilated area during the Corning Lambda EliteMax Benchtop Pipettor’s
operation. Keep the power supply away from high traffic areas.
Connect the AC Power Line
While connecting the AC power line, the user should insert the plug firmly to ensure good contact between the plug and socket.
Pull the plug, but not the cable, when the plug needs to be disconnected from the mains.

2| CORNING LAMBDA ELITEMAX SEMI-AUTOMATED BENCHTOP PIPETTOR
Design Environments
The Corning® Lambda™ EliteMax Benchtop Pipettor should be placed in a low-humidity, dust-free, well ventilated room without
caustic gas, corrosive vapors, or powerful magnetic interference. The Corning Lambda EliteMax Benchtop Pipettor should never
be operated near water sources such as pools and water pipes. Never cover or obstruct the openings of the Corning Lambda
EliteMax Benchtop Pipettor as they are designed for ventilation and prevention of the device’s interior from becoming too hot.
When the instrument is running, the shortest distance between its openings and the nearest object should be at least 20 inches
(50 cm). Do not place the device on a soft surface. Place the device on a stable, level bench or hood. Excessive jarring or vibration
during operation may affect the device’s performance. Operating conditions that cause a high temperature environment will
result in the degraded performance or failure of the Corning Lambda EliteMax Benchtop Pipettor. Additionally, the device
should be protected against any kind of heat sources such as sunlight, ovens, or central heating equipment. If the Corning
Lambda EliteMax Benchtop Pipettor is to sit idle for extended periods of time, it is recommended to disconnect the power line
from the mains, and cover the device with a piece of soft cloth or plastic to prevent against dust.
CAUTION: If any of the following events occur, the operator is advised to disconnect the power cable from the mains, and
contact your local Corning representative or ask a certified maintenance engineer for assistance.
Liquid enters the device.
The device malfunctions, giving off an abnormal sound or odor.
The device falls onto the floor or the housing is damaged.
There are significant changes in the device’s performance.
1.4 Instrument Identication
Touch Screen
Controller
Instrument Deck
Disposable Tips
and Plates
(not included)
Liquid Head
Single-channel Head Multi-channel Head
Manually Operated
Tip Ejector

Instruction Manual | 3
1.5 Deck Positions and Orientation Convention
CAUTION: Before running any protocols, make sure ALL tip racks, plates, reservoirs, and accessories share the same orientation
to avoid possible collision that can damage the instrument or the sample plate.
If there is any apparent damage to the system, the user should investigate the nature of the damage before plugging the unit into
the mains to ensure that the powering up of the system will not create a hazardous condition or damage internal components. If
the damage appears significant, call your local Corning representative before connecting the unit to the mains.

4| CORNING LAMBDA ELITEMAX SEMI-AUTOMATED BENCHTOP PIPETTOR
Typical landscape layout orientation.
Typical portrait layout orientation.
NOTE: Portrait adapter between plates.
1.6 Package Contents
Cat. No. Description Qty/Pk
6070 Corning® Lambda™ EliteMax Semi-automated Benchtop Pipettor 1
6071 Touch screen controller with software and license 1
6073 External power supply 1
6104 Portrait adapter stainless steel plate 1
–Power cords (US, EU, UK, CHN) 1
–Instruction manual 1
–Calibration certificate 1

Instruction Manual | 5
1.7 Specications
Performance Specifications
Pipetting Channels Single-channel and 12-channel for 96-well format
Volume Range 1 µL to 200 µL
Plate Formats 96-well standard, shallow, deep well, microcentrifuge, V-, U-, and flat-bottom
Functions and Protocols Plate fillings, serial dilutions, plate-to-plate transfers, plate replication, sample preparation,
cherry picking protocol (for single-channel head only), and other more complex protocols can be
implemented
Accuracy
20 µL <±2.0%
100 µL <±1.5%
200 µL <±1.0%
Precision
20 µL <1.5% CV
100 µL <1.0% CV
200 µL <0.7% CV
NOTE: The instrument uses a programmable calibration algorithm, where aspiration, dispense, and liquid speed can be adjusted for a
particular reagent. Precision and accuracy can be highly improved for a particular reagent and transfer volume.
Precision shown as percentage of coefficient of variation.
Multi-channel specifications represent the performance of all channels of a pipet.
All values shown as reference. Corning EliteMax tips used. See NOTE above.
Technical Data
Dimensions (W x D x H)
Main Unit
Touch screen controller
External power supply
External power cord
Touch screen controller cable
20.5 x 11.3 x 14.2 in. (520 x 285 x 360 mm)
11.3 x 6.3 x 5.3 in. (284 x 160 x 135 mm)
55 in. (140 cm)
72 in. (183 cm)
60 in. (152.4 cm)
Weight
Main Unit
Touch screen controller
44 lbs. (20 kg)
3.3 lbs. (1.5 kg)
Power Requirements
Main power supply
Device input
100-240 VAC, 47-63 Hz
24 VDC, 120W (US, EU, UK, and CHN plug types included)
Operating Temperature 5°C to 40°C (relative humidity 80% up to 30°C or 45% relative humidity at 40°C)
Storage Temperature -10°C to +50°C

6| CORNING LAMBDA ELITEMAX SEMI-AUTOMATED BENCHTOP PIPETTOR
1.8 Initial Installation
To install the unit:
1. Connect the Touch Screen Controller cable into the rear panel of the Corning® Lambda™
EliteMax Benchtop Pipettor.
2. Connect the round connector of the universal external power supply into the rear panel of
the instrument. Push in firmly until you feel it click into position.
3. Connect the AC power cord to the universal external power supply and then to the AC wall
power receptacle. The AC power receptacle should be able to supply approx. 300W and
MUST be grounded to a true earth ground.
4. Power up the unit by rocking the Reset/Off switch located in the back of the Corning
Lambda EliteMax Benchtop Pipettor to the Reset position. The instrument should boot up,
and the home screen will appear in a few seconds.
2.0 Instrument Operation
2.1 Touch Screen User Interface
2.1.1 Main Menu
After powering up the Corning Lambda EliteMax Benchtop Pipettor, you will be presented with the main screen or Home Screen.
Simply touch the desired button to access the submenu.
Plate Filling: Create, run, save, or edit a Plate Filling protocol.
Serial Dilution: Create, run, save, or edit a Serial Dilution protocol.
Plate to Plate Transfer: Create, run, save, or edit a Plate-to-Plate Transfer protocol.
Single Channel: Create, run, save, or edit a Single-channel protocol (this is also known as cherry picking with the single-channel head only).
Settings: Change instrument settings, access calibration data and other configuration tools.
My Files: View user-created protocols and data logged information. From here you are also able to delete unwanted protocols.
Log In: Login under a specific user profile. To access this menu, a user profile must be created first in Settings.
1
2
4

Instruction Manual | 7
2.1.2 Numeric and Alphanumeric Keypad
To change settings, touch the text box, dropdown menu, or other control that you wish to interact with. A numeric or alphanumeric
keypad will automatically appear next to the selected field or control as shown.
If you are unable to edit or change values, the screen is most likely locked or disabled so that you don’t accidentally change any
important values or settings. To enable the screen, just click or touch on the More Options drop down menu, and select Edit
User Program (Protocol Toolbar). Now you should be able to edit or change any desired values. Every time you open or save a
protocol, the screen is automatically disabled for safety reasons.
Numeric keypad
Alphanumeric keypad
Tip

8| CORNING LAMBDA ELITEMAX SEMI-AUTOMATED BENCHTOP PIPETTOR
2.1.3 Protocol Toolbar Description
All protocols share the same top Toolbar as shown here.
Menu Item Description
Use to exit your current page and return to the main menu.
Use this menu to:
Edit the current loaded user protocol.
Open user created protocols.
Save user-created protocols.
Delete user-created protocols.
Reset Next Available Tips (NAT). See more details under the Protocol General
Settings section.
Reset reservoir VOLUME tracking feature. See more details under the Protocol
General Settings section.
Switch current logged on user.
Use to move to a different user protocol without going back to the Home
Screen. This feature works as a shortcut to a different user protocol.
The status window shows the current status of the instrument and the User
Name of the logged-on user.
Use the Run button to execute the current protocol.
CAUTION: Verify that the tip rack and other necessary plates/reservoirs
are in the correct positions before pressing Run. Running the protocol
without the appropriate placement can result in the instrument
crashing and possible damage to the instrument or plate sample.

Instruction Manual | 9
2.1.4 Log In User Information
This feature allows different users to share the instrument while avoiding unintentional modification or deletion of user protocols. If
this feature is enabled, the user needs to enter their credentials to operate the instrument.
After pressing the Log In button, the following screen appears where the user can enter their credentials and log in. User Name and
assword fields are case-sensitive. The login screen and toolbar will show if there is a current logged-on user.
After using the instrument, the user can log out from this same screen.
This feature allows different users to share the instrument while avoiding unintentional modification.
NOTE: The instrument is shipped with security disabled.
See Settings/Users, User Management module Section 3.6 to manage users and to enable or disable this feature.
Just in case, the factory default login credentials are included here:
Username: labmanager
Password: 12341234
It is important that you change or delete the factory default credentials once you have set up at least one other administrative user in
the User Management module. Make sure that the newly created user has administrative privileges, or they will not be able to access
the Settings menu.
2.1.5 My Files User Menu
After pressing the My Files button, the following screen will appear:
In this screen, saved protocols can be seen in a clearer more readable text format. Press the corresponding button to perform the
desired task. NOTE: Deleting files requires Delete permissions which are assigned by an administrator (if this feature is enabled). See
Settings/Users Section 3.6 to manage users and to enable or disable these privileges.

10 | CORNING LAMBDA ELITEMAX SEMI-AUTOMATED BENCHTOP PIPETTOR
2.2 Creating and Running User Protocols
2.2.1 Plate Filling
By pressing the Plate Filling button in the main menu, the following screen will appear:
NOTE: The Plate Filling protocol contains two tabs: General Settings and Plate Filling Protocol. See Table 1 for more details.
If you are unable to edit or change values, the screen is most likely locked or disabled so that you don’t accidentally change any
important values or settings. To enable the screen, just click or touch on the More Options drop down menu and select Edit
User Program (Protocol Toolbar). Now you should be able to edit or change any desired values. Every time you open or save a
protocol, the screen is automatically disabled for safety reasons.
Table 1. General Settings Tab Menu: Plate Filling
Menu Item Description
Name of the current protocol.
Use to set the aspirate and dispense liquid head speed.
Use Low speed for viscous solutions and when working with cells.
Use higher speeds when using thinner solutions or reagents. NOTE: The higher
the speed, the faster the protocol will be completed.
When this box is checked, it allows the user to specify the following protocol
to be executed once the current one is completed. See Linked File Information
(Table 2) for more details.
Utilize this dropdown menu to select the rack of tips to be used with this
protocol.
Tip

Instruction Manual | 11
Table 1. General Settings Tab Menu: Plate Filling (continued)
Menu Item Description
When this box is checked, the instrument will pick up a new row of tips every
time a different row or column is accessed on the microplate.
Use to select how the disposable tips are going to be consumed.
When Specific Row is selected, tips are picked up always from the same
location. Use the plate image on the right side of the screen to specify the
tip’s location.
When Next Available Row is selected, the instrument will pick up the first
unused row of tips from the rack. This is automatically tracked by the
instrument in the NAT (Next Available Tip) feature. To reset NAT, select More
Options/Reset NAT located in the toolbar.
When Always Ask is selected, the instrument will prompt the user for a tip
row each time the protocol is executed.
Linked File Information:
You can link as many protocols as necessary to perform your experiment. The link file settings will be saved with the protocol, and
the linking will occur each time the protocol is executed.
NOTE: The following sub-menu appears only when the Link File box is checked.
It is recommended that you create all of your protocols first, and then add the links between them. This helps to avoid errors
caused by trying to link to a protocol that doesn’t exist.
Specify the name of the protocol to be executed when the current protocol is
completed. If the protocol already exists, click on the Browse button to select
the file you want to link. You can also type in the name of the file.
Specify a time delay value in seconds between completion of the current
protocol and the start of the next linked protocol.
When this box is checked, the instrument will perform a full Home axis routine.
When this box is checked, the instrument will pause between protocols until
the operator instructs the instrument to continue.
NOTE: This setting will override any value in the Link File Delay field.
Table 2. Plate Filling Protocol Tab Menu
Menu Item Description
Use to select the plate to be filled.
Specify the volume [µL] that you want to transfer from the reagent reservoir
into the plate wells.
This is the volume of air [µL] to be displaced after all transfer volume has
been dispensed into the wells.
This feature helps to dispense all excess liquid that remains in the tip after
each dispense. The air volume should be sufficient to assist the separation
of the droplet from the tip into the well, but not so great that air bubbles
become a problem.
The instrument automatically calculates the maximum air volume possible
based on the stroke specified in Tip Air Gap (see Settings). If you enter a very
large volume, the instrument will use the maximum air volume accessible.
The Blowout parameter is most often optimized by trial and error.
Tip

12 | CORNING LAMBDA ELITEMAX SEMI-AUTOMATED BENCHTOP PIPETTOR
Table 2. Plate Filling Protocol Tab Menu (continued)
Menu Item Description
Specify whether or not there is liquid in the target cells before the filling
occurs. The instrument uses this information to determine the liquid
meniscus height to properly position the tips for optimal liquid transfers.
Enter a value to determine how many times the mix is performed on each
row after the liquid transfer has occurred.
Enter a volume [µL] that you would like to aspirate and dispense when
performing the mixes specified in the previous step.
When this box is checked, the instrument will withdraw enough reagent
from the reservoir to fill specified rows without having to refill each time. The
instrument will automatically calculate how much is needed to fill wells with
the specified volume. Allow Aliquot Transfer option speeds up the plate filling
protocol.
Use to select the reservoir that you want to withdraw from.
Enter a value to determine how many times the mix is performed in the
reservoir before any liquid transfer occurs.
Enter a volume [µL] that you would like to aspirate and dispense when
performing the mixes in the reservoir.
Use to determine reservoir condition at start.
Fix volume at start. Enter a value here [mL]. Every time this protocol is
executed, the instrument assumes that the reservoir contains the specified
amount of reagent.
When Track Volume is selected, the instrument will keep track of the
remaining reagent left in the reservoir. When there is not enough reagent
in the reservoir, the instrument will stop and ask the user to refill it. To
reset the reservoir volume, select More Options/Reset VOLUME from the
toolbar.
When Always Ask is selected, the instrument will prompt the user for the
reservoir volume each time the protocol is executed.
Touch a row on the plate image to select or deselect a row to be filled.
Only rows that have been highlighted in green will be filled.
CAUTION: Verify that the tip rack and other necessary plates/reservoirs are in the correct positions before pressing Run. Running
the protocol without the appropriate placement can result in the instrument crashing and possible damage to the instrument
or plate sample.
To save the protocol, select More Options/Save User Program from the toolbar.
To execute the protocol, press Run on the toolbar.

Instruction Manual | 13
At any time while the instrument is running, you can press the Stop button located at the bottom left of the Runtime Screen.
This will pause the instrument and give you the option to abort or to continue the protocol.
After pressing the Run button, the instrument checks for protocol errors or inconsistency such as the filling of a row that will cause
the well to overflow. If any error or inconsistency is detected, the instrument will warn you and give you the opportunity to correct the
issue.
While executing the protocol, the Runtime Screen will be displayed. This screen shows the status of each operation performed by the
instrument in real time. The wells in the plate image, located on the right side of the screen, will turn green as they are filled.
2.2.2 Serial Dilution
By pressing the Serial Dilution button in the main menu, the following screen will appear:

14 | CORNING LAMBDA ELITEMAX SEMI-AUTOMATED BENCHTOP PIPETTOR
NOTE: The Serial Dilution protocol contains two tabs: General Settings and Serial Dilution Protocol. See Table 3 for more details.
If you are unable to edit or change values, the screen is most likely locked or disabled so that you don’t accidentally change
any important values or settings. To enable the screen, just click or touch on the More Options dropdown menu and select Edit
User Program (Protocol Toolbar). Now you should be able to edit or change any desired values. Every time you open or save a
protocol, the screen is automatically disabled for safety reasons.
Table 3. General Settings Tab Menu: Serial Dilution Protocol
Menu Item Description
Name of the current protocol.
Use to set the aspirate and dispense liquid head speed.
Use Low speed for viscous solutions and when working with cells.
Use higher speeds when using thinner solutions or reagents. NOTE: The higher
the speed, the faster the protocol will be completed.
When this box is checked, it allows the user to specify the following protocol
to be executed after the current one is completed. See Linked File Information
(Table 4) for more details.
Use to select the rack of tips to be used in this user protocol.
Use to select where to dispose the used tips after the serial dilution is
completed.
When this box is checked, the instrument will pick up a new row of tips every
time a different row or column is accessed on the microplate.
Use to select how the disposable tips are going to be consumed.
When Specific Row is selected, tips are always picked up from the same
location. Use the plate image on the right side of the screen to choose the
tip’s location.
When Next Available Row is selected, the instrument will pick up the first
unused row of tips from the rack. This is automatically tracked by the
instrument in the NAT (Next Available Tip) feature. To reset NAT, select
More Options/Reset NAT from the toolbar.
When Always Ask is selected, the instrument will prompt the user for a tip
row each time the protocol is executed.
Linked File Information:
You can link as many protocols as necessary to perform your experiment. The link file settings will be saved with the protocol, and
the linking will occur each time the protocol is executed.
NOTE: The following sub-menu appears only when the Link File box is checked.
It is recommended that you create all of your protocols first, and then add the links between them. This helps to avoid errors
caused by trying to link to a protocol that doesn’t exist.
Specify the name of the protocol to be executed when the current protocol is
completed. If the protocol already exists, click on the Browse button to select
the file you want to link. You can also type in the name of the file.
Specify a time delay value in seconds between completion of the current
protocol and the start of the next linked protocol.
Tip
Tip

Instruction Manual | 15
Table 3. General Settings Tab Menu: Serial Dilution Protocol (continued)
Menu Item Description
When this box is checked, the instrument will perform a full Home axis
routine.
When this box is checked, the instrument will pause between protocols until
the operator instructs the instrument to continue. This setting will override
any value in the Link File Delay field.
Table 4. Serial Dilution Protocol Tab Menu
Menu Item Description
Use to select the plate to be filled.
Use to specify the volume [µL] that you want to transfer when performing
the serial dilution.
This is the volume of air [µL] to be displaced after all transfer volume has
been dispensed into the wells.
This feature helps to dispense all excess liquid that remains in the tip after
each dispense. The air volume should be sufficient to assist the separation
of the droplet from the tip into the well, but not so great that air bubbles
become a problem.
The instrument automatically calculates the maximum air volume possible,
based on the stroke specified in Tip Air Gap (see Settings). If you enter a very
large volume, the instrument will use the maximum air volume accessible.
The Blowout parameter is most often optimized by trial and error.
Enter the volume [µL] that is already present in the “source” wells.
The instrument uses this information to determine the liquid meniscus
height to properly position the tips for optimal liquid transfers.
Enter the volume [µL] that is already present in the wells to be diluted.
The instrument uses this information to determine the liquid meniscus
height to properly position the tips for optimal liquid transfers.
Enter a value to determine how many times the mix is performed in each row
before any liquid transfer occurs.
Enter a value to determine how many times the mix is performed in each row
after the liquid transfer occurs.
Enter a volume [µL] that you would like to aspirate and dispense when
performing the mixes specified in the previous steps.
Select where you want the instrument to dispense the carried-over reagent
after the serial dilution is completed.
Source Well: The first accessed row of the serial dilution protocol.
Last Diluted Row: The last accessed row of the serial dilution protocol.
Magazine Tips: Dispose of carried-over reagent in the tips rack when
returning or ejecting tips.
Reservoir: Use the dropdown menu to select a reagent reservoir.

16 | CORNING LAMBDA ELITEMAX SEMI-AUTOMATED BENCHTOP PIPETTOR
Table 4. Serial Dilution Protocol Tab Menu (continued)
Menu Item Description
Touch a row on the plate image to select or deselect a row to be filled. Only
rows that have been highlighted in green will be accessed.
NOTE: The serial dilution is always executed from top to bottom (A-H). The
first row, from top to bottom, is considered the SOURCE row. As shown in the
image, it’s possible to skip rows while performing a serial dilution. The same
principle applies when working with plates in portrait orientation (1-12), top
to bottom.
CAUTION: Verify that the tip rack and other necessary plates/reservoirs are in the correct positions before pressing run. Running
the protocol without the appropriate placement can result in the instrument crashing and possible damage to the instrument
or plate sample.
To save the protocol, select More Options/Save User Program from the toolbar.
To execute the protocol, press Run on the toolbar.
At any time while the instrument is running, you can press the Stop button located at the bottom left of the Runtime screen.
This will pause the instrument and give you the option to abort or to continue the protocol.
After pressing the Run button, the instrument checks for protocol errors or inconsistency such as the filling of a row that will cause
the well to overflow. If any error or inconsistency is detected, the instrument will warn you and give you the opportunity to correct the
issue.
While executing the protocol, the Runtime screen will be displayed. This screen shows the status of each operation performed by the
instrument in real time. The wells in the plate image, located on the right side of the screen, will turn green as they are filled.

Instruction Manual | 17
2.2.3 Plate-to-Plate Transfers
By pressing the Plate-to-Plate Transfer button in the main menu, the following screen will appear:
NOTE: The Plate-to-Plate Transfer protocol contains two tabs: General Settings and Plate-to-Plate Protocol. See Table 5 for more
details.
If you are unable to edit or change values, the screen is most likely locked or disabled so that you don’t accidentally change any
important values or settings. To enable the screen, just click or touch on the More Options drop down menu and select Edit
User Program (Protocol Toolbar). Now you should be able to edit or change any desired values. Every time you open or save a
protocol, the screen is automatically disabled for safety reasons.
Table 5. General Settings Tab Menu: Plate-to-Plate protocol
Menu Item Description
Name of the current protocol.
Use to set the aspirate and dispense liquid head speed.
Use Low speed for viscous solutions and when working with cells. Use higher
speeds when using thinner solutions or reagents. NOTE: The higher the
speed, the faster the protocol will be completed.
When this box is checked, it allows the user to specify the following protocol
to be executed after the current one is completed. See Linked File Information
below for more details.
Use to select the rack of tips to be used with this protocol.
When this box is checked, the instrument will pick up a new row of tips every
time a different row or column is accessed on the microplate.
Tip

18 | CORNING LAMBDA ELITEMAX SEMI-AUTOMATED BENCHTOP PIPETTOR
Table 5. General Settings Tab Menu: Plate-to-Plate protocol (continued)
Menu Item Description
Use to select how the disposable tips are going to be consumed.
When Specific Row is selected, tips are always picked up from the same
location. Use the plate image on the right side of the screen to choose the
tip’s location.
When Next Available Row is selected, the instrument will pick up the first
unused row of tips from the rack. This is automatically tracked by the
instrument in the NAT (Next Available Tip) feature. To reset NAT, select
More Options/Reset NAT from the toolbar.
When Always Ask is selected, the instrument will prompt the user for a tip
row each time the protocol is executed.
Linked File Information:
You can link as many protocols as necessary to perform your experiment. The link file settings will be saved with the protocol, and
the linking will occur each time the protocol is executed.
NOTE: The following sub-menu appears only when the Link File box is checked.
It is recommended that you create all of your protocols first, and then add the links between them. This helps to avoid errors
caused by trying to link to a protocol that doesn’t exist.
Specify the name of the protocol to be executed when the current protocol is
completed. If the protocol already exists, click on the Browse button to select
the file you want to link. You can also type in the name of the file.
Specify a time delay value in seconds between completion of the current
protocol and the start of the next linked protocol.
When this box is checked, the instrument will perform a full Home axis
routine.
When this box is checked, the instrument will pause between protocols until
the operator instructs the instrument to continue. This setting will override
any value in the Link File Delay field.
Table 6. Plate-to-Plate Protocol Tab Menu
Menu Item Description
Select the plate you want to withdraw from or the Source plate.
Enter the volume [µL] that is already present in the Source wells.
The instrument uses this information to determine the liquid meniscus
height to properly position the tips for optimal liquid transfers.
Select the plate where you want to dispense to or Destination Plate. If you
want to transfer within the same plate, select the same plate you selected
previously in Source Plate.
Enter the volume [µL] that is already present in the Destination Plat wells.
The instrument uses this information to determine the liquid meniscus
height to properly position the tips for optimal liquid transfers.
Use to specify the volume [µL] that you want to transfer from the Source
Plate to the Destination Plate.
Tip