CORNING 6928 User manual

Corning®
X-WASH®System
Instruction Manual
Catalog Numbers:
6928
6929
6930
6931
6932
6933

3
Instruction Manual
Table of Contents
Introduction .................................................... 4
Safety. .......................................................... 9
Corning® X-WASH® System......................................14
X-SERIES® DataTrak Software ...................................22
Corning X-WASH System Operating Instructions .................28
X-SERIES DataTrak Software: System Setup ......................51
X-SERIES DataTrak Administration Mode .........................53
X-SERIES DataTrak Operations Mode ............................59
Maintenance, Cleaning, and Disinfection ........................63
Troubleshooting................................................64
Technical Support ..............................................67
Specifications ..................................................67
Product Disposal ...............................................74
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
11.0
12.0
13.0

4CORNING X-WASH SYSTEM
1.0 Introduction
The Corning® X-WASH® System is a semi-automated, functionally closed,
system that separates, washes, and volume-reduces cell samples to a
programmable volume. The X-WASH System is used with a commercially
available centrifuge and personal computer (not supplied).
The system consists of four major components:
• Corning X-WASH Disposable Cartridge, single-use
• Corning X-WASH Control Module, rechargeable
• Corning X-WASH Docking Station, AC-powered
• X-SERIES® DataTrak Software
The Corning X-WASH Control Module component of the X-WASH System
is a microprocessor-controlled device, which works in combination with the
Corning X-WASH Disposable Cartridge during centrifugation to direct the
transfer of cells into a separate compartment within the Disposable Cartridge.
After centrifugation, the X-WASH Control Module is placed on the Corning
X-WASH Docking Station to download processing data using the X-SERIES
DataTrak Software.
Intended Use
The Corning X-WASH System is general lab equipment to be used to wash
and/or concentrate cell suspensions.
Overview
For the purposes of the intended use, the input volume is limited to a range
of approximately 40 mL to 240 mL, with an output max (or cell concentrate
volume) of approximately 40 mL. Average processing time is approximately
17 minutes (single wash).
The sample of thawed blood along with thaw media to be processed is
placed in the sterile, functionally closed X-WASH Disposable Cartridge for
processing. High-speed and low-speed centrifugation is used to separate
cells from cryoprotectant using a commercially available programmable
centrifuge, such as the Thermo Scientific Sorvall™ Legend™ XT or XTR, and
the Hettich ROTANTA 460.
NOTE: For the purpose of this instruction manual, thawed blood samples
will be used in all examples and process steps, with the goal of collecting
a cell fraction that has been washed and resuspended. Thawed blood
demonstrates the basic system operation. For information about specific
processing differences or requirements for other cellular products, please
contact Corning Scientific Support at USA/Canada: 1.800.492.1110 Option
3, +1.978.442.2200 Option 3, or email [email protected].
Outside of the US, visit www.corning.com/lifesciences or contact your local
support office (see the back of instruction manual).

5
Instruction Manual
During the initial high-speed portion of the centrifugation (2,000 RCF), the
cells in the thawed blood pellet at the base of the cone in the Disposable
Cartridge. The speed is then lowered to 50 x g, and the cells are directed
to the Disposable Cartridge harvest compartment, which has been pre-
filled with resuspension media. The bulk of media and cryopreservative is
retained in the central compartment of the cartridge.
Once the centrifuge cycle ends, the operator places the Control Module on
the Docking Station and follows the DataTrak software screen instructions
to download the processing data. The battery within the Control Module is
also recharged for the next blood separation procedure.
Symbol Keys
The following symbols and abbreviations are used on the Corning®
X-WASH® System labeling, packaging, or within this instruction manual.
Sterile uid path Sterile uid path
using irradiation
Expiration date Do not re-use
Non-pyrogenic Do not trash as municipal waste
Indicates that a product has
been evaluated to applicable
European directives
Batch code
Catalog number Serial number
Manufacturer Caution to be observed by the
user
Warning to be observed
by the user Temperature limit
Keep dry Humidity
Pressure Do not use if package is
damaged
Follow Instructions for Use Direct Current
Consult Instructions for Use

6CORNING X-WASH SYSTEM
Glossary of Definitions and Abbreviations
Below are abbreviations that are used in this Instruction Manual and their
associated definitions.
Term Denition
Cell Concentrate The targeted concentration of white blood cells separated from cryoprotectant
and media.
Control Module Provides the mechanisms that open and close the valve on the Disposable
Cartridge to direct components of blood to their respective compartments.
CSV Comma Separated Values
DataTrak Software Proprietary data management system which collects processing and system
information through a user interface and stores it in a searchable, sortable
database.
Disposable Cartridge Single-use device with a sterile, non-pyrogenic uid path.
Docking Station Provides communication link to DataTrak Software and enables charging of
the device battery.
IEC International Electrotechnical Commission
LED Light Emitting Diode
PC Personal Computer
PDF Portable Document Format
Corning® X-WASH®
System
Cell processing system that includes: Disposable Cartridge, Control Module,
Docking Station, and DataTrak Software
RCF Relative Centrifugal Force or g-force
RF Radio Frequency

7
Instruction Manual
Warranties
Corning Incorporated has reviewed the contents of this manual. All
statements, technical information and recommendations contained in
this manual are current and believed to be reliable. Manual contents are
not intended to be and should not be understood as representations or
warranties for the Corning® X-WASH® System. The contents of this manual
are provided “as is” and without warranties of any kind, either express or
implied.
The Corning X-WASH System is covered by specific and limited warranties,
as described in this chapter.
For questions about Corning X-WASH System warranties, or if warranty
repairs are needed, contact an authorized distributor or Corning
Incorporated.
Fill out the box below for future reference:
Distributor Name __________________________________________________________
Contact Name _____________________________________________________________
Email ____________________________________________________________________
Phone Number_____________________________________________________________
Hours of Operation _________________________________________________________
Corning Incorporated
Life Sciences
836 North St.
Building 300, Suite 3401
Tewksbury, MA 01876
t 800.492.1110
t 978.442.2200
f 978.442.2476
Scienti[email protected]
www.corning.com/lifesciences
Outside of the U.S., visit www.corning.com/lifesciences or contact your local
support office (see the back of instruction manual).
When calling for service or repairs, have available:
• Detailed information about the problem
• Serial numbers and date/place of purchase of Control Module and
Docking Station
• Disposable lot number and expiration date
• Service record given at time of training

8CORNING X-WASH SYSTEM
Limited Warranty
Corning warrants to the original purchaser that the Corning® X-WASH®
Control Module and X-WASH Docking Station (referred to as a “X-WASH
Instrument”) will be free from defects in material or workmanship for one
(1) year from date of shipment. Installation documents must be completed
and returned to Corning for the warranty to be recognized. Failure to
provide such documentation may result in the warranty being considered
null and void.
In the event that an X-WASH Instrument does not conform to the foregoing
warranty, Corning will, at its option, repair the nonconforming X-WASH
Instrument, provide a free replacement, or grant you an appropriate credit.
These alternatives shall be your sole and exclusive remedy in the event of a
failure to comply with the foregoing warranty.
Corning also warrants that replacement parts of the X-WASH Instruments
will be free from defects in material and workmanship for the remaining
portion of the original warranty, or, if longer, the minimum time required by
applicable local law, from date of shipment of replacement part.
X-WASH Instruments may fail or malfunction for reasons other than those
caused by defects in materials or workmanship (such as improper handling
of the machine, misuse, accident, or non-compliance with the Instruction
Manual. Any such failure is not covered under the Corning warranty and
such X-WASH Instrument will be replaced or repaired at the cost of the end-
user.
Any alterations or modification made to an X-WASH Instrument (other than
those made by Corning) will render the warranty null and void.
Corning shall under no circumstances be liable to you or any other party,
under any circumstances, for any special, consequential, indirect or punitive
damages such as loss of capital, loss of use, substitute performance, loss of
production, loss of profits, loss of business opportunity, or any other claims
for damages, even if such losses or damages are reasonably foreseeable.
THE FOREGOING WARRANTIES, AS SET FORTH HEREIN, ARE EXCLUSIVE
AND ARE IN LIEU OF, AND YOU HEREBY WAIVE, ALL OTHER WARRANTIES,
EXPRESS OR IMPLIED, INCLUDING, WITHOUT LIMITATION, ANY IMPLIED
WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR
PURPOSE OR WARRANTY OF NON-INFRINGEMENT.

9
Instruction Manual
2.0 Safety
Warnings
A WARNING is a statement preceded by this symbol that alerts
the user to the possibility of injury, death, or other serious adverse
reactions associated with the use or misuse of the device.
• Read this instruction manual completely prior to using the Corning®
X-WASH® System.
• Always follow internal policy for personal protective equipment such as
gloves, gowns, eyewear, or masks when using the X-WASH System.
• Always follow established procedures for handling and discarding
biohazardous materials.
• The X-WASH System has been optimized for 40 mL to 240 mL input
volume and other volumes will yield unknown results.
• Operators should be trained on the X-WASH System prior to initial use
• Follow aseptic procedures.
• The Corning X-WASH Disposable Cartridge is a single-use device with a
sterile, non-pyrogenic fluid path. Do not use if packaging or product is
damaged. Do not re-sterilize. Follow internal policy for proper disposal of
used Disposable Cartridge.
• No modification of this equipment is allowed. Only Corning authorized
replacement parts may be used.
• MEDICAL/LABORATORY ELECTRICAL EQUIPMENT needs special
precautions regarding EMC and needs to be installed and put into service
according to the EMC information provided in this manual.
• Do not exceed 2,000 RCF centrifugation at any time as an inappropriate
transfer of cellular components between compartments may occur,
causing a loss of cell functionality requiring the sample to be discarded.
Cautions
A CAUTION is a statement preceded by this symbol that alerts the
user to the possibility of a problem with the device associated with
its use or misuse. Such problems include device malfunction, device
failure, damage to the device, or damage to other property. The
caution statement includes the precaution that should be taken to
avoid the hazard.
• Operators should be trained on the Corning X-WASH System prior
to initial use.
• Do not insert any objects, including fingers, into the Corning X-WASH
Control Module while powered on or at any other time.

10 CORNING X-WASH SYSTEM
• Follow aseptic procedures.
• Avoid liquid contact with the X-WASH Control Module internal
components. In the event that liquids enter the X-WASH Control Module,
contact Corning.
• Handle the X-WASH Control Module and Docking Station with care.
• When connected to another device, the overall system must be evaluated
to IEC 60601-1 3rd edition by the end user.
• Avoid dropping any of the components of the X-WASH System as this
may cause damage.
• Keep the X-WASH Disposable Cartridge upright during and after filling
to prevent fluids from entering the 0.2 micron air filter as this may affect
airflow through the filter.
• The X-WASH Control Module battery should be charged in the X-WASH
Docking Station overnight or for at least 6 hours prior to initial use.
• Only use the X-SERIES® DataTrak Software for downloading and storage
of processing information.
• Ensure that the computer is virus-free and has the latest update of
antivirus software prior to installing DataTrak Software.
• Update the operating system, service pack, and antivirus software on a
regular basis.
• Do not leave an internet connection active while installing or using
DataTrak.
• The user-provided Sample Label barcode must be in ISBT or code 128
format to be compatible with the DataTrak Software.
• Prior to starting centrifugation, ensure the following:
• Battery status indicator on the Corning Control Module is green when
placed on the Corning Docking Station
• Corning® X-WASH® Control Module is OFF before latching the X-WASH
Disposable Cartridge
• Corning X-WASH Disposable Cartridge is fully latched into the X-WASH
Control Module
• Control Module is turned ON and “0” is displayed in the STATUS
DISPLAY window before centrifugation.
• Prior to cleaning, power down the X-WASH Control Module, and unplug
the X-WASH Docking Station.
• The X-WASH System has been optimized for 40 mL to 240 mL input
volume and other volumes will yield unknown results.

11
Instruction Manual
Standards and Regulations
The Corning® X-WASH® System was tested and found to meet all
regulations and recommendations contained in this document, which are
in compliance with the appropriate international safety standards. This
includes electrical instruments used in laboratory, protection of laboratory
staff and electrical safety.
EMC Compliance Information
Unit complies with requirements within the European Union’s
Electromagnetic Compatibility (EMC) Directive 2014/30/EU. Limits are
designed to provide reasonable protection against harmful interference
in a typical facility. This equipment generates, uses and can radiate
radio frequency energy, and, if not installed and used in accordance with
manufacturer’s instructions, may cause harmful interference to other
devices in the vicinity. There is no guarantee, however, that interference
will not occur in a particular facility. If this equipment causes interference
with other devices, which may be determined by turning equipment off and
on, user is encouraged to try and correct interference by one or more of the
following measures:
• Reorient or relocate device receiving interference
• Increase separation between equipment
• Connect equipment to a different outlet than the one connected to the
other device
• Consult manufacturer or field service technician for help
MEDICAL/LABORATORY ELECTRICAL EQUIPMENT needs special
precautions regarding EMC and needs to be installed and put into
service according to the EMC information provided in this manual.

12 CORNING X-WASH SYSTEM
NOTE: The EQUIPMENT is intended for use in the electromagnetic
environment specified below. The customer or the user of the EQUIPMENT
should assure that it is used in such an environment.
Guidance and Manufacturer’s Declaration – Electromagnetic Emissions for All Manufacturer’s
Equipment and Manufacturer’s Systems
Emissions Test Compliance Electromagnetic Environment Guidance
RF emissions CISPR 11 Group 1 The Corning® X-WASH® System uses RF energy only
for its internal function. Therefore, its RF emissions
are very low and are not likely to cause any interfer-
ence in nearby electronic equipment.
RF emissions CISPR 11 Class B The Corning X-WASH System is suitable for use in all
establishments, including domestic establishments
and those directly connected to the public low voltage
power supply network that supplies buildings used for
domestic purposes.
Harmonic emissions
IEC 61000-3-2
Class A
Voltage uctuations/icker
emissions IEC 61000-3-3
Complies
Guidance and Manufacturer’s Declaration – Electromagnetic Immunity for All Manufacturer’s
Equipment and Manufacturer’s Systems
Immunity Test IEC 60601 Test Level Compliance Level Electromagnetic Environment
Guidance
Electrostatic discharge
(ESD) IEC 61000-4-2
±6 kV contact
±8 kV air
±6 kV contact
±8 kV air
Floors should be wood, con-
crete, or ceramic tile. If oors
are covered with synthetic
material, the relative humidity
should be at least 30%.
Electrical fast transient/
burst IEC 61000-4-4
±2 kV for power
supply lines
±1 kV for input/
output lines
±2 kV for power
supply lines
±1 kV for input/
output lines
Mains power quality should be
that of a typical commercial or
hospital environment.
Surge
IEC 61000-4-5
±1 kV line(s) to
line(s)
±2 kV line(s) to earth
±1 kV line(s) to
line(s)
±2 kV line(s) to
earth
Mains power quality should be
that of a typical commercial or
hospital environment.
Voltage dips, short
interruptions and volt-
age variations on power
supply input lines IEC
61000-4-11
<5% UT (>95% dip in
UT) for 0,5 cycles
40% UT (60% dip in
UT) for 5 cycles
70% UT (30% dip in
UT) for 25 cycles
<5% UT (>95% dip in
UT) for 5 s
<5% UT (>95%
dip in UT) for 0,5
cycles
40% UT (60% dip
in UT) for 5 cycles
70% UT (30%
dip in UT) for 25
cycles
<5% UT(>95% dip
in UT) for 5 s
Mains power quality should be
that of a typical commercial
or hospital environment. If the
user of the Corning X-WASH
System requires continued
operation during power mains
interruptions, it is recommend-
ed that the X-WASH System be
powered from an uninterrupt-
ible power supply or a battery.
Power frequency (50/60
Hz) magnetic eld IEC
61000-4-8
3 A/m 3 A/m Power frequency magnetic
elds should be at levels char-
acteristic of a typical location in
a typical commercial or hospital
environment.
NOTE: UT is the A.C. mains voltage prior to application of the test level.

13
Instruction Manual
NOTE: The EQUIPMENT is intended for use in the electromagnetic
environment specified below. The customer or the user of the EQUIPMENT
should assure that it is used in such an environment.
Guidance and Manufacturer’s Declaration – Electromagnetic Immunity for Equipment and Systems that
are not Life Supporting
Immunity Test IEC 60601 Test Level Compliance Level Electromagnetic Environment
Guidance
Conducted RF
IEC 61000-4-6
Radiated RF
IEC 61000-4-3
3 Vrms
150 kHz to 80 MHz
3 V/m
80 MHz to 2,5 GHz
3 Vrms
3 V/m
Portable and mobile RF communications
equipment should be used no closer
to any part of the Corning® X-WASH®
system, including cables, than the
recommended separation distance cal-
culated from the equation applicable
to the frequency of the transmitter.
Recommended separation distance
d = 1.2 √P
d = 1.2 √P 80 MHz to 800 MHz
d = 2.3 √P 800 MHz to 2,5 GHz
Where P is the maximum output
power rating of the transmitter in
watts (W) according to the transmitter
manufacturer and d is the recommend-
ed separation distance in meters (m).
Field strengths from xed RF transmit-
ters, as determined by an electromag-
netic site surveya, should be less than
the compliance level in each frequency
rangeb.
Interference may occur in the vicinity
of equipment marked with the follow-
ing symbol:
NOTE 1: At 80 MHz and 800 MHz, the higher frequency range applies.
NOTE 2: These guidelines may not apply in all situations. Electromagnetic propagation is affected by
absorption and reection from structures, objects and people.
aField strengths from xed transmitters, such as base stations for radio (cellular/cordless) telephones
and land mobile radios, amateur radio, AM and FM radio broadcast and TV broadcast cannot be predict-
ed theoretically with accuracy. To assess the electromagnetic environment due to xed RF transmitters,
an electromagnetic site survey should be considered. If the measured eld strength in the location
in which the X-WASH System is used exceeds the applicable RF compliance level above, the X-WASH
System should be observed to verify normal operation. If abnormal performance is observed, additional
measures may be necessary, such as re-orienting or relocating the X-WASH System.
bOver the frequency range 150 kHz to 80 MHz, eld strengths should be less than 3 V/m.

14 CORNING X-WASH SYSTEM
NOTE: The X-WASH System is intended for use in an electromagnetic
environment in which radiated RF disturbances are controlled. The customer
or the user of the X-WASH System can help prevent electromagnetic
interference by maintaining a minimum distance between portable and
mobile RF communications equipment (transmitters) and the X-WASH
System as recommended below, according to the maximum output power
of the communications equipment.
Recommended Separation Distances Between Portable and Mobile RF Communications Equipment and
the Corning X-WASH System
Rated Maximum
Output Power of
Transmitter W
Separation Distance According to Frequency of Transmitter M
150 kHz to 80 MHz
d = 1.2 √P
80 MHz to 800 MHz
d = 1.2 √P
800 MHz to 2,5 GHz
d = 2.3 √P
0.01 0.12 0.12 0.23
0.1 0.38 0.38 0.73
1 1.2 1.2 2.3
10 3.8 3.8 7.3
100 12 12 23
For transmitters rated at a maximum output power not listed above, the recommended separation
distance d in meters (m) can be estimated using the equation applicable to the frequency of the
transmitter, where P is the maximum output power rating of the transmitter in watts (W) according
to the transmitter manufacturer.
NOTE 1: At 80 MHz and 800 MHz, the separation distance for the higher frequency range applies.
NOTE 2: These guidelines may not apply in all situations. Electromagnetic propagation is affected by
absorption and reection from structures, objects and people.
3.0 Corning® X-WASH® System
The Corning X-WASH System is composed of the following components:
Cat. No. Description
5712 Corning X-WASH Disposable Cartridge
6928 Corning X-WASH Control Module with associated rmware
6929 Corning X-WASH Docking Station with associated rmware
6930 X-SERIES® DataTrak Software
Corning provides the following suggested products for use with the
Corning X-WASH System:
Cat. No. Description
6923 Corning X-Balance Ring Kit
6934 Corning X-Mixer
6935 Corning X-WASH Accessory Kit
6922 Low
Corning X-Counterweight6921 Medium
6920 High

15
Instruction Manual
The Corning® X-WASH® System requires the following instrumentation for
operation. These are not provided by Corning:
• Programmable centrifuge with 750 mL buckets (Thermo Scientific
Sorvall™ Legend™ XT or XTR, Hettich ROTANTA 460)
• Computer (e.g., desktop, laptop PC, or tablet) with Windows® 7 or 10
Operating System and at least one available USB port (additional USB
port required for optional barcode scanner)
• Tube Sealer (Genesis Rapid Seal II™ SE540)
Materials and equipment that are required but not supplied:
• 40 mL to 240 mL of cellular sample to be processed
• Biological safety cabinet or clean room area
• Digital scale
• Label printer(s) for component labeling (if applicable)
• Syringes for sample (if applicable)
• Labels for components and sample tubes (if applicable)
Corning X-WASH Disposable Cartridge
The Corning X-WASH Disposable Cartridge is a dedicated sterile, plastic,
single-use processing container and comprises the fluid path of the system.
The thawed sample and thaw media is transferred into the Disposable
Cartridge through a 150 micron clot filter. The Corning Disposable Cartridge
is latched into the Corning X-WASH Control Module for subsequent
centrifugation following a preprogrammed profile described below.
Integrated within the Disposable Cartridge is a mechanism that pinches or
releases the tubing, allowing for transfer of the cellular components during
centrifugation. The Control Module manages this operation.
Blood Thaw Wash Centrifuge Profile Example
• The centrifuge accelerates to 1,000 RCF to allow the cell suspension to
pellet into the cone at the base of the Disposable Cartridge.
• The centrifuge then lowers to 50 RCF and the pelleted cells are
transferred through transfer tubing to the Harvest Compartment leaving
the bulk of supernatant retained in the processing compartment.
• The centrifuge decelerates and stops spinning, the washed cells can now
be removed.

16 CORNING X-WASH SYSTEM
Corning® X-WASH® Disposable Cartridge Components
Needleless Port A
Needleless
Port B
Air Filter
Disposable
Cartridge Housing
Clamps
Dual Bag Spikes
The components of the Corning X-WASH Disposable Cartridge are
described below:
• Air Filter: A 0.2 micron hydrophobic filter that facilitates air management
during filling.
• Disposable Cartridge Housing: Contains the individual harvest
compartments, the central funnel compartment, and integrated pinch
mechanisms.
• Needleless Port A is used for input of thaw media.
• Needleless Port B is used for removal of sample from sample pillow.
• Sample Access Tube is used for the collection of harvested cells and is
located on the opposite side of the lot number label (not shown).
• Sample Output Luer Lock is a female luer lock through which the harvest
is removed by syringe (not shown).
• Dual Bag Spikes are used for insertion into the sample bag
• Clot Filter: A 150 micron filter to remove particles from cellular input
during sample input.
• Pinch Clamps located throughout the input line are used to control fluid
flow.
Clamp
Clot Filter

17
Instruction Manual
Corning® X-WASH® Control Module
The Corning X-WASH Control Module is a battery-operated, microprocessor-
controlled, electromechanical device with two motor/gear/cam assemblies
that rotate in order to pinch or release tubing in the Corning Disposable
Cartridge to direct the cell concentrate to the harvest compartment.
The X-WASH Control Module is also equipped with an accelerometer to
measure acceleration and four optical sensors that detect interfaces cell
concentrate and the supernatant, all powered by a lithium ion battery. The
paired sensors can detect the boundary between the supernatant and cell
concentrate when the passage of infrared light is blocked, then unblocked.
The Control Module uses the detected boundary to open and close the
transfer tubes in the Disposable Cartridge. The Control Module’s firmware
gathers and stores the accelerometer’s output and the timing and release
of the pinch mechanism during each processing run for upload to the
X-SERIES DataTrak Software.
Corning X-WASH Control Module Components
Electrical Connectors
Device Information Label
Serial Number Label
User Interface Label
Orientation Point Optical Sensors/
Receivers Latch Point
for Disposable
Cartridges
• Optical Sensors and Receivers: Four optical sensors and receivers that
detect the cell interfaces during centrifugation
• Latch Point for Disposable Cartridges: The two connections for latching
the Disposable Cartridge to the Control Module
• Disposable Cartridge Orientation Points: The three points for the
Disposable Cartridge feet that insure correct orientation when
connecting
• Device Information Label: Provides information specific to the Control
Module as well as Corning contact information
• Electrical Connectors: The connectors that make contact with the
Docking Station and facilitate data transmission and the Control Module
battery charging

18 CORNING X-WASH SYSTEM
• Serial Number Label: Control Module barcode and human readable text
label
• User Interface Label: Contains the power button, as well as displays
battery status, error codes, and power status
Corning User Interface Label
Battery Status Indicator Power Button Status Display
Attention IndicatorPower Status Indicator
Battery Status Indicator
When power button is pressed, steady green indicates that the battery has
a sufficient charge to perform a harvest process.
When power button is pressed, steady red indicates that the battery is
insufficiently charged. The operator should immediately charge the battery.
When the Control Module is placed on the Docking Station for charging,
the Battery Status Indicator will blink at the following rates in response to
battery charge levels:
• Green 1-blink pattern Leave on Docking Station to charge
• Green 2-blink pattern Charge soon
• Green 3-blink pattern Ready to use
• Green Steady Fully charged and ready to use
Power Button
• Press this button for approximately one (1) second to power ON the
Control Module.
• Press and hold for approximately three (3) seconds to power OFF the
Control Module.
Power Status Indicator
• Green indicates that the Control Module is powered ON.
• No illumination indicates that the Control Module is powered OFF.

19
Instruction Manual
Status Display
Displays the status or error code of the Control Module. Standard Status
Codes are described below. Refer to Troubleshooting (Chapter 10) for error
codes and additional information.
Character Denition Process Description
0Ready to operate The Control Module is ready for processing
P Pass The process is has completed successfully
dP Data present The data needs to be downloaded in preparation for a new
processing run
Attention Indicator
• Green indicates that the Control Module is ready for processing or has
successfully completed a centrifugation process.
• Red indicates that the Control Module has failed a centrifugation
process, has gone into an error state, or the Control Module was powered
down prior to downloading the data. The operator should assess which
of these situations has occurred, and take appropriate remedial actions
(see Troubleshooting Chapter 10)
Corning® X-WASH® Docking Station
The Corning X-WASH Docking Station is a multifunctional system
component that concurrently charges the battery of the Corning Control
Module and enables transmission of data to and from the Control Module
onto a host Windows® computer.
Docking Station Components
The components of the Docking Station are described below:
• Connector Pins: Retractable pins which align and contact with electrical
connectors on the bottom of the docked Control Module for data
communication and battery charging.
• User Interface Label: Identifies the device and allows the user to see an
LED with three states:
• Green indicates that a Control Module is present and charging.
• Red indicates that no Control Module is present, or the Control Module is
present but not seated properly, or Control Module is not functional. The
operator should assess which of these situations has occurred and take
appropriate actions to remedy.
• No illumination indicates that the Docking Station is not receiving power.
• USB Data Port: Port for connection to the host computer via the USB cable.
• Power Cord Port: Port for connection to the external power source via the
power cable. To remove mains power, unplug the cable from the wall.
Connector Pins
User Interface Label
USB Data Port
Power Cord Port
USB Cable

20 CORNING X-WASH SYSTEM
• Device Information Label: Provides information specific to the Docking
Station as well as Corning contact information.
• USB Cable: Connects the Docking Station to the Windows Computer or
tablet to allow for the transfer of Control Module data.
Accessories
Corning® X-Balance Ring Kit
The Corning X-Balance Rings are specifically designed for use with the
Corning X-WASH® System for balancing the contents of the centrifuge cup
prior to centrifugation. They are provided in 2.5 and 7.4 gram weights.
Corning X-Counterweights
The Corning X-Counterweight set is specifically designed for use with the
Corning X-WASH System for balancing the contents of the centrifuge prior
to centrifugation. Three different weights: LOW, MEDIUM, and HIGH are
provided to accommodate a range of different input volumes.
X-Counterweight LOW MEDIUM HIGH
Cat. No. 6922 6921 6920
Input Volume (mL) 40 to 110 111 to 170 171 to 240
Image
Device Information
Label
Link

21
Instruction Manual
The Disposable Cartridge Holder holds the Disposable Cartridge during the
mixing cycles.
The Resuspend Button resuspends pelleted cells after the supernatant has
been removed.
The Continuous Button mixes larger volumes within the Disposable Cartridge.
The Fan prevents the X-Mixer from overheating.
On/Off Power Switch turns the X-Mixer on or off. Press to turn on (I) or off (O).
The Power Cord Connection is used for connection to the external power
source via AC/DC adapter and the power cord.
The Air tubing with male connector attaches to the air filter of the Disposable
Cartridge and a peristatic pump to facilitate fluid transfer in and out of the
Disposable Cartridge.
Disposable Cartridge caps help prevent the air filter on the Disposable
Cartridge from getting wet during mixing.
Pinch Clamps are used to control fluid flow in and out of the Disposable
Cartridge.
Disposable
Cartridge Holder
On/Off
Switch
Product/
Serial Label
Power Supply
Connection
Resuspend
Button
Fan
FRONT BACK
Continuous
Button
Corning® X-Mixer
Corning® X-WASH® Accessory Kit
Air tubing with
male connector (2x)
Disposable
Cartridge caps (2x)
Clamps (2x)