Fisher Scientific accuSpin 3 User manual

accuSpin™ 3 / 3R
OPERATING
INSTRUCTIONS

How to use this manual
Use this manual to get acquainted with your centri-
fuge and its accessories.
The manual helps you to avoid inappropriate han-
dling. Make sure to keep it always close to the centri-
fuge.
A manual that is not kept handy cannot provide
protection against improper handling and thus
against damage to persons and objects.
The manual comprises chapters on
• Safety regulations
• Instrument description
• Rotor program and accessories
• Transportation and hook-up
• Use of the centrifuge
• Maintenance and care
• Troubleshooting
• Technical data
• Index
Please fold out
Overleaf you will find a graphic
representation of the control panel
with a survey of the most important
functions

speed/RCF
Quick run
run time
open lid
stop
start
The mains switch is located on the right-hand
side panel.
Pressed up = ON I
Pressed down = OFF 0
(only for instruments with refrigeration unit)
temperature
program selection
Bucket selection
rpm/RCF
switch key
display "set" keys
"set" keys
pre-temperature-regulation
Before switching on the centrifuge
please read this manual
deceleration
profiles
acceleration
profiles

Control panel of the accuSpin™ 3 / 3R
Display panels
Program selection
Key 1 - 4 : store or recall programs.
Acceleration profiles (1= slowest ...... 9= fastest)
permanent
display: acceleration profile last set 1- 9
Deceleration profiles (1= slowest ...... 9= fastest)
permanent
display: deceleration profile last set 1- 9
Speed / RCF
run: current value of speed or RCF after activation of
switch key
end: "End"
lid open: "OPEN"
"Lift Lid" (if lid is not automatically lifted off)
(before start)
lid closed: "0" with flashing point
(rotor not yet identified)
error code: will flash in display
Run time
time selection: - remaining run time to 0
continuous - run time passed (HLD)
operation (hld) (in hours, minutes)
"Quick Run": - run time passed (as long as button is held;
in minutes and seconds)
Temperature*
run: current sample temperature in °C
(in temperature equilibrium)
* only for instruments with refrigeration unit
Keys
start : normal start of the centrifuge
stop : manual stop of a run
open lid: open lid (possible only with the instrument
switched on)
Quick run: short-term operation of the centrifuge as long as
key remains pressed
rpm/RCF
switch: switching between rpm and RCF display
Bucket set.: Setting of the bucket number
Pretemp : Pre-temp-function*
"set" keys: stepwise increase/decrease of setpoint values
Short pressing of any of the "set" keys: switch from current to preset
value, signalled by flashing display.
Error codes (troubleshooting see chapter "Troubleshooting"):
E-19: Unpermissible rotor
E-20: Rotor not identified
E-29: Motor or rotor blocked
E-31: Overtemperature in motor
E-32: Overtemperature in electronic
E-33: Excessive pressure in the refrigeration unit
E-34: Overvoltage
E-35: Overcurrent
Lift Lid : Smoothly lift the lid
rotor: set speed higher than permissible speed of
the rotor
bAL : unbalance

Contents
1
Contents
For your safety............................................ 3
Proper use................................................................ 3
Improper use ............................................................ 3
Centrifuging hazardous materials ............................ 3
Handling the centrifuge ............................................ 4
Conformity to current standards............................... 5
Safety instructions in this manual ............................ 5
The accuSpin™ 3 / 3R ................................ 7
Description ............................................................... 7
Safety systems......................................................... 8
Parts supplied .......................................................... 8
Function and features .............................................. 9
Before use................................................. 13
Centrifuge transport and installation ...................... 13
Proper location ....................................................... 13
Main connection ..................................................... 14
Rotors and accessories ........................... 15
Rotors for the accuSpin™ 3................................... 17
Rotors for the accuSpin™ 3R................................. 18
Handling the rotor................................................... 23
Aerosol-tight operation........................................... 25
Checking of aerosol-tight bio-containment......... 27
Operation .................................................. 29
Switching on the centrifuge .................................... 29
Actuating the lid...................................................... 29
Opening the lid ................................................... 29
Closing the lid ..................................................... 29
Installing the rotor................................................... 30
Loading the rotor .................................................... 31
Maximum loading ............................................... 31
Filling the centrifuge tubes ................................. 31
Maximum permissible load difference................ 32
Inserting the centrifuge tubes ............................. 32
Entering parameters............................................... 35
Deceleration curves ............................................... 35
Switching from speed to RCF display .................... 35
Bucket selection for swinging bucket rotors........... 35
Selecting speed...................................................... 36
Entering the RCF value.......................................... 36
More about the RCF value ................................. 37
Selecting run time .................................................. 37
Run time selection.............................................. 37
Continuous operation ......................................... 38
Extended time mode........................................... 38
Selecting the temperature ...................................... 39
Pretemp function .................................................... 39
Starting the centrifuge ............................................ 40

Contents
2
Imbalance display................................................... 40
Changing the settings during the run ..................... 40
Stopping the centrifuge .......................................... 41
Stopping with preset run time............................. 41
Stopping with continuous operation ................... 41
Temperature control during standby ...................... 41
Working with programs........................................... 42
Program display.................................................. 42
Entering/changing a program ............................. 42
Centrifuging with a program ............................... 42
”Quick Run” ............................................................ 43
Removing the rotor................................................. 43
Audible alarm ......................................................... 44
Turning the centrifuge off ....................................... 44
Maintenance and care .............................. 45
Maintenance to be performed by the customer ..... 45
Cleaning.............................................................. 45
Disinfection ......................................................... 46
Decontamination................................................. 48
Autoclaving ......................................................... 48
Troubleshooting ....................................... 49
Emergency lid release............................................ 49
Error troubleshooting.............................................. 51
Contacting Fisher ScientificService..................... 60
Technical Data .......................................... 61
Electrical connections / fuses................................. 63
Appendix ................................................... 65
Acceleration and deceleration profiles ................... 65
Speed/RCF diagrams............................................. 70
Index .......................................................... 73

For your safety
3
For your safety
Fisher Scientificcentrifuges are manufactured accord-
ing to current technical standards and regulations.
Nonetheless, centrifuges may pose danger to individu-
als and surrounding if
• they are not used as designed
• they are operated by untrained personnel
• their design is improperly changed
• the safety instructions are not followed
Therefore, personnel involved with operation and
maintenance of the centrifuge must read and follow
the safety instructions.
In addition, the pertinent regulations for prevention of
accidents must be strictly followed
This manual is an integral part of the centri-
fuge assembly and must be kept close at
hand at all times.
When damages at the power cord or at
casing were noticed the centrifuge has
to be set out of operation!
Proper use
The centrifuge is designed to separate liquid-
suspended materials having different densities and
particle size, respectively (maximum sample density is
1.2 g/cm³ {ml} at maximum speed).
Improper use
During a run, a safety zone of 30 cm around the centri-
fuge must be maintained where neither persons nor
hazardous materials may be present.
The centrifuge may cause harm to user or other per-
sons or may damage goods if safety measures are not
followed:
Centrifuging hazardous materials
• The centrifuge is neither made inert, nor is it explo-
sion-proof. Therefore never use the centrifuge in an
explosion-prone environment.
• Do not centrifuge explosive or flammable sub-
stances must not be centrifuged. The same holds
for substances prone to react violently with each
other.

For your safety
4
• Do not centrifuge toxic or radioactive substances or
pathogenic microorganisms without suitable safety
systems.
If microbiological samples of risk group II (according
to "Laboratory Bio-safety Manual" of WHO) are be-
ing centrifuged, aerosol-tight bio-seals have to be
used.
For materials with a higher risk group, more than
one precaution is required.
• Should toxins or pathogenic substances enter the
centrifuge or its parts, you must perform appropriate
procedures for disinfection (see "Maintenance and
care – Disinfection").
• Strongly corrosive substances that may cause
damage to materials and reduce the mechanical
strength of the rotor, may be centrifuged only inside
protective tubes.
Handling the centrifuge
• Use only original accessories for the centrifuge. The
only exception are common glass or plastic centri-
fuge tubes, if they are approved for the rotor speed
and RCF values.
• Never use the centrifuge unless the rotor is properly
installed.
• You may use the centrifuge only with a properly
loaded rotor. You must not overload the rotor.
• Strictly follow the rules and regulations for cleaning
and disinfection
• If the rotor or the rotor lid shows signs of corrosion
or wear, you must stop using it.
• Never open the lid manually if the rotor still turns.
• You may use the emergency lid release only in
case of emergency, e.g. during an interruption of
power supply
(see chapter "Troubleshooting").
• Never use the centrifuge with the lid open.
• Never use the centrifuge if the front panel has been
partially or totally removed.
• Changes in mechanical or electrical components of
the centrifuge may be carried out only by individuals
authorized by Fisher Scientific.

For your safety
5
Conformity to current standards
Fisher Scientificcentrifuges are manufactured and
tested according to the following standards and regula-
tions:
- for all voltages
• IEC 61010
- for 120 V only
•
- for 230 V only
•
Details of the test standards take you please from the
technical data.
Safety instructions in this manual
This symbol denotes potential hazards to
persons.
This symbol denotes potential damage to
the centrifuge or parts in its immediate
surroundings.
General hints are marked with this
symbol.

For your safety
6
Notes