cytiva TE62 User manual

TE62 Standard Transfer Tank
Operating Instructions
Original instructions
cytiva.com

Table of Contents
1Introduction ........................................................................................................ 3
1.1 About this manual ......................................................................................................................................... 4
1.2 Important user information ....................................................................................................................... 5
2 Safety instructions ............................................................................................. 6
2.1 Safety precautions ........................................................................................................................................ 7
2.2 Labels ................................................................................................................................................................. 13
2.3 Emergency procedures ............................................................................................................................... 14
3 System description ............................................................................................ 15
4 Installation .......................................................................................................... 18
5 Operation ............................................................................................................ 19
5.1 Preparations .................................................................................................................................................... 21
5.2 Assembly ........................................................................................................................................................... 23
5.3 Electrotransfer ................................................................................................................................................ 26
6 Maintenance ....................................................................................................... 29
7 Troubleshooting ................................................................................................. 31
8 Reference information ....................................................................................... 35
8.1 Specifications .................................................................................................................................................. 36
8.2 Recycling information .................................................................................................................................. 37
8.3 Regulatory information ............................................................................................................................... 38
8.3.1 Contact information .................................................................................................................................... 39
8.3.2 European Union and European Economic Area .............................................................................. 40
8.3.3 Eurasian Economic Union
Евразийский экономический союз .................................................................................................. 41
8.3.4 Declaration of Hazardous Substances (DoHS) ................................................................................ 43
8.4 Ordering information ................................................................................................................................... 45
8.5 Health and Safety Declaration Form ...................................................................................................... 46
Index ........................................................................................................................... 48
Table of Contents
2 TE62 Standard Transfer Tank Operating Instructions 29281927 AB

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, and
intended use of the Transphor instrument.
In this chapter
Section See page
1.1 About this manual 4
1.2 Important user information 5
1 Introduction
TE62 Standard Transfer Tank Operating Instructions 29281927 AB 3

1.1 About this manual
Purpose of this manual
The Operating Instructions provide you with the information needed to install, operate
and maintain the product in a safe way.
Scope of this manual
The Operating Instructions cover the Transphor instrument. The illustration below
shows the Transphor instrument.
Illustrations
The images and annotations in this document are for illustrative purposes only. The
configuration of individual products may vary, and therefore illustrations may not
reflect the actual system delivered.
1 Introduction
1.1 About this manual
4 TE62 Standard Transfer Tank Operating Instructions 29281927 AB

1.2 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documenta-
tion. If you do, you may be exposed to hazards that can lead to personal injury and you
may cause damage to the equipment.
Intended use of the product
Transphor transfers proteins, DNA, or RNA from up to four polyacrylamide or agarose
gels onto a membrane. Gels and membranes are held by a cassette, which is
submerged into the transfer tank. Molecules migrate under an electric field to the
membrane, where they are bound.
The transfer buffer temperature can be controlled by circulating cooled liquid through
the heat exchanger in the base. The buffer is separated from the coolant by a heatcon-
ducting alumina plate.
Transphor must not be used in any clinical procedures, or for diagnostic purposes.
Prerequisites
In order to operate Transphor in the way it is intended:
•The user must have a general understanding of electrophoresis and blotting techni-
ques.
•The user must read and understand the Safety Instructions chapter in the Oper-
ating Instructions.
•Transphor must be installed in accordance with the site requirements and instruc-
tions in the Operating Instructions.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
1 Introduction
1.2 Important user information
TE62 Standard Transfer Tank Operating Instructions 29281927 AB 5

2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition, the chapter describes emergency and recovery procedures.
Important
WARNING
Before installing, operating or maintaining the product, all
users must read and understand the entire contents of this
chapter to become aware of the hazards involved.
In this chapter
Section See page
2.1 Safety precautions 7
2.2 Labels 13
2.3 Emergency procedures 14
2 Safety instructions
6 TE62 Standard Transfer Tank Operating Instructions 29281927 AB

2.1 Safety precautions
Introduction
Transphor is powered by an external power supply and handles materials that are
considered hazardous material. Before installing, operating or maintaining the system,
you must be aware of the hazards described in this manual.
Follow the instructions provided to avoid injury to the operator or other
personnel, to the product, or to other equipment in the area.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Personal protection
•Using flammable liquids
•Installing and moving the product
•Power supply
•Operation
•Maintenance
Always follow the instructions below to avoid injury when using the Transphor.
Definitions
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
2 Safety instructions
2.1 Safety precautions
TE62 Standard Transfer Tank Operating Instructions 29281927 AB 7

General precautions
WARNING
Before installing, operating or maintaining the product, all
users must read and understand the entire contents of this
chapter to become aware of the hazards involved.
WARNING
Only properly trained personnel may operate and maintain the
product.
WARNING
Do not operate the Transphor in any other way than described in
the Transphor Operating Instructions.
WARNING
Do not damage the power supply cord by bending, twisting, heating
or allowing it to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.
If the power supply cords are damaged, contact your local Cytiva
representative for replacements.
WARNING
The safety lid must be in place before connecting the power leads
to a power supply.
WARNING
Any liquid on the equipment must be dried off before connecting
the power supply.
CAUTION
The product is designed for indoor use only.
2 Safety instructions
2.1 Safety precautions
8 TE62 Standard Transfer Tank Operating Instructions 29281927 AB

Personal protection
WARNING
Always use appropriate Personal Protective Equipment (PPE)
during operation and maintenance of this product.
WARNING
Hazardous substances and biological agents. When using
hazardous chemical and biological agents, take all suitable protec-
tive measures, such as wearing protective clothing, glasses and
gloves resistant to the substances used. Follow local and/or
national regulations for safe operation and maintenance of this
product.
WARNING
Spread of biological agents. The operator must take all neces-
sary actions to avoid spreading hazardous biological agents. The
facility must comply with the national code of practice for
biosafety.
CAUTION
Handle the glass components with care! Wear appropriate
personal protective equipment (PPE).
Using flammable liquids
WARNING
A fume hood or similar ventilation system shall be installed when
flammable or noxious substances are used.
Installing and moving the product
CAUTION
Turn off the power switch and remove connecting cables before
moving the equipment.
2 Safety instructions
2.1 Safety precautions
TE62 Standard Transfer Tank Operating Instructions 29281927 AB 9

CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
CAUTION
When lifting and moving the instrument be careful not to drop it.
This may cause injury.
CAUTION
The electrophoresis unit is heavy, especially when filled with buffer.
Handle the unit with care to avoid personal injury.
Power supply
WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by Cytiva.
WARNING
Make sure that there is access to the instrument power supply cord
at all times.
Operation
WARNING
The high voltage power supply must always be disconnected when
the safety lid of the electrophoresis unit is taken off. The high
voltage power supply must never be switched on unless the safety
lid is on the electrophoresis unit.
WARNING
Never exceed the operating limits stated in this document and on
the system label. Operation of the product outside these limits can
damage equipment and cause personal injury or death.
2 Safety instructions
2.1 Safety precautions
10 TE62 Standard Transfer Tank Operating Instructions 29281927 AB