cytiva TE62 User manual

TE62 Standard Transfer Tank
Operating Instructions
Original instructions
cytiva.com

Table of Contents
1Introduction ........................................................................................................ 3
1.1 About this manual ......................................................................................................................................... 4
1.2 Important user information ....................................................................................................................... 5
2 Safety instructions ............................................................................................. 6
2.1 Safety precautions ........................................................................................................................................ 7
2.2 Labels ................................................................................................................................................................. 13
2.3 Emergency procedures ............................................................................................................................... 14
3 System description ............................................................................................ 15
4 Installation .......................................................................................................... 18
5 Operation ............................................................................................................ 19
5.1 Preparations .................................................................................................................................................... 21
5.2 Assembly ........................................................................................................................................................... 23
5.3 Electrotransfer ................................................................................................................................................ 26
6 Maintenance ....................................................................................................... 29
7 Troubleshooting ................................................................................................. 31
8 Reference information ....................................................................................... 35
8.1 Specifications .................................................................................................................................................. 36
8.2 Recycling information .................................................................................................................................. 37
8.3 Regulatory information ............................................................................................................................... 38
8.3.1 Contact information .................................................................................................................................... 39
8.3.2 European Union and European Economic Area .............................................................................. 40
8.3.3 Eurasian Economic Union
Евразийский экономический союз .................................................................................................. 41
8.3.4 Declaration of Hazardous Substances (DoHS) ................................................................................ 43
8.4 Ordering information ................................................................................................................................... 45
8.5 Health and Safety Declaration Form ...................................................................................................... 46
Index ........................................................................................................................... 48
Table of Contents
2 TE62 Standard Transfer Tank Operating Instructions 29281927 AB

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, and
intended use of the Transphor instrument.
In this chapter
Section See page
1.1 About this manual 4
1.2 Important user information 5
1 Introduction
TE62 Standard Transfer Tank Operating Instructions 29281927 AB 3

1.1 About this manual
Purpose of this manual
The Operating Instructions provide you with the information needed to install, operate
and maintain the product in a safe way.
Scope of this manual
The Operating Instructions cover the Transphor instrument. The illustration below
shows the Transphor instrument.
Illustrations
The images and annotations in this document are for illustrative purposes only. The
configuration of individual products may vary, and therefore illustrations may not
reflect the actual system delivered.
1 Introduction
1.1 About this manual
4 TE62 Standard Transfer Tank Operating Instructions 29281927 AB

1.2 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documenta-
tion. If you do, you may be exposed to hazards that can lead to personal injury and you
may cause damage to the equipment.
Intended use of the product
Transphor transfers proteins, DNA, or RNA from up to four polyacrylamide or agarose
gels onto a membrane. Gels and membranes are held by a cassette, which is
submerged into the transfer tank. Molecules migrate under an electric field to the
membrane, where they are bound.
The transfer buffer temperature can be controlled by circulating cooled liquid through
the heat exchanger in the base. The buffer is separated from the coolant by a heatcon-
ducting alumina plate.
Transphor must not be used in any clinical procedures, or for diagnostic purposes.
Prerequisites
In order to operate Transphor in the way it is intended:
•The user must have a general understanding of electrophoresis and blotting techni-
ques.
•The user must read and understand the Safety Instructions chapter in the Oper-
ating Instructions.
•Transphor must be installed in accordance with the site requirements and instruc-
tions in the Operating Instructions.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
1 Introduction
1.2 Important user information
TE62 Standard Transfer Tank Operating Instructions 29281927 AB 5

2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition, the chapter describes emergency and recovery procedures.
Important
WARNING
Before installing, operating or maintaining the product, all
users must read and understand the entire contents of this
chapter to become aware of the hazards involved.
In this chapter
Section See page
2.1 Safety precautions 7
2.2 Labels 13
2.3 Emergency procedures 14
2 Safety instructions
6 TE62 Standard Transfer Tank Operating Instructions 29281927 AB

2.1 Safety precautions
Introduction
Transphor is powered by an external power supply and handles materials that are
considered hazardous material. Before installing, operating or maintaining the system,
you must be aware of the hazards described in this manual.
Follow the instructions provided to avoid injury to the operator or other
personnel, to the product, or to other equipment in the area.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Personal protection
•Using flammable liquids
•Installing and moving the product
•Power supply
•Operation
•Maintenance
Always follow the instructions below to avoid injury when using the Transphor.
Definitions
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
2 Safety instructions
2.1 Safety precautions
TE62 Standard Transfer Tank Operating Instructions 29281927 AB 7

General precautions
WARNING
Before installing, operating or maintaining the product, all
users must read and understand the entire contents of this
chapter to become aware of the hazards involved.
WARNING
Only properly trained personnel may operate and maintain the
product.
WARNING
Do not operate the Transphor in any other way than described in
the Transphor Operating Instructions.
WARNING
Do not damage the power supply cord by bending, twisting, heating
or allowing it to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.
If the power supply cords are damaged, contact your local Cytiva
representative for replacements.
WARNING
The safety lid must be in place before connecting the power leads
to a power supply.
WARNING
Any liquid on the equipment must be dried off before connecting
the power supply.
CAUTION
The product is designed for indoor use only.
2 Safety instructions
2.1 Safety precautions
8 TE62 Standard Transfer Tank Operating Instructions 29281927 AB

Personal protection
WARNING
Always use appropriate Personal Protective Equipment (PPE)
during operation and maintenance of this product.
WARNING
Hazardous substances and biological agents. When using
hazardous chemical and biological agents, take all suitable protec-
tive measures, such as wearing protective clothing, glasses and
gloves resistant to the substances used. Follow local and/or
national regulations for safe operation and maintenance of this
product.
WARNING
Spread of biological agents. The operator must take all neces-
sary actions to avoid spreading hazardous biological agents. The
facility must comply with the national code of practice for
biosafety.
CAUTION
Handle the glass components with care! Wear appropriate
personal protective equipment (PPE).
Using flammable liquids
WARNING
A fume hood or similar ventilation system shall be installed when
flammable or noxious substances are used.
Installing and moving the product
CAUTION
Turn off the power switch and remove connecting cables before
moving the equipment.
2 Safety instructions
2.1 Safety precautions
TE62 Standard Transfer Tank Operating Instructions 29281927 AB 9

CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
CAUTION
When lifting and moving the instrument be careful not to drop it.
This may cause injury.
CAUTION
The electrophoresis unit is heavy, especially when filled with buffer.
Handle the unit with care to avoid personal injury.
Power supply
WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by Cytiva.
WARNING
Make sure that there is access to the instrument power supply cord
at all times.
Operation
WARNING
The high voltage power supply must always be disconnected when
the safety lid of the electrophoresis unit is taken off. The high
voltage power supply must never be switched on unless the safety
lid is on the electrophoresis unit.
WARNING
Never exceed the operating limits stated in this document and on
the system label. Operation of the product outside these limits can
damage equipment and cause personal injury or death.
2 Safety instructions
2.1 Safety precautions
10 TE62 Standard Transfer Tank Operating Instructions 29281927 AB

WARNING
Turn all power supply controls off and disconnect the power leads
before removing the safety lid.
WARNING
Rinse only the electrodes (not the banana plugs) with distilled
water before use.
CAUTION
Circulate coolant through the heat exchanger to minimize heating.
Overheating will cause irreparable damage to the unit! Do not
connect the heat exchanger to a water tap or any coolant source
where the water pressure is unregulated.
CAUTION
Circulate only water or 50/50 water/ethylene glycol through the
heat exchanger. Never use anti-freeze or any organic solvent in the
heat exchanger.
CAUTION
Never introduce anti-freeze or any organic solvent into any part of
the instrument. Organic solvents will cause irreparable damage to
the instrument.
CAUTION
Buffer above the maximum buffer level line may cause corrosion of
the electrical contacts.
2 Safety instructions
2.1 Safety precautions
TE62 Standard Transfer Tank Operating Instructions 29281927 AB 11

Maintenance
WARNING
Decontaminate before maintenance. To avoid personnel being
exposed to potentially hazardous substances, make sure that the
Transphor is properly decontaminated and sanitized before main-
tenance or service.
WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by Cytiva may be used for maintaining or
servicing the product.
WARNING
Disconnect power. Always disconnect power from the instru-
ment before performing any maintenance task.
WARNING
Decommissioning. Decontaminate the equipment before
decommissioning to ensure that hazardous residues are removed.
2 Safety instructions
2.1 Safety precautions
12 TE62 Standard Transfer Tank Operating Instructions 29281927 AB

2.2 Labels
Introduction
This section describes the system label and other safety or regulatory labels that are
attached to the product.
Description of symbols on the system
label
The table below describes the various symbols that may be found on the system label.
Label Meaning
Warning! Read the user documentation before using the system.
Do not open any covers or replace parts unless specifically stated
in the user documentation.
Serial no.: Serial number of the product
Manufac-
tured:
Year (YYYY) and month (MM) of manufacture
Safety labels
The table below describes the various symbols that may be found on the product.
Symbol/text Description
Warning! Read the user documentation before using
the system. Do not open any covers or replace parts
unless specifically stated in the user documentation.
Warning! High Voltage. Always make sure that the
system is disconnected from electric power before
removing the lid.
2 Safety instructions
2.2 Labels
TE62 Standard Transfer Tank Operating Instructions 29281927 AB 13

2.3 Emergency procedures
Introduction
This section describes how to shut down the product in an emergency situation, and
the procedure for restarting the product.
The section also describes the result in the event of power failure.
Safety precautions
WARNING
Make sure that there is access to the instrument power supply cord
at all times.
Emergency shutdown
In an emergency situation, shut down the power supply in accordance with its emer-
gency procedure.
Power failure
In case of power failure to the product, the run is interrupted immediately.
Restart after emergency shutdown or
power failure
To restart the run after an emergency shutdown or power failure, follow these steps:
Step Action
1Make sure all connections are in place.
2Start the power supply as described in the power supply's User Manual.
2 Safety instructions
2.3 Emergency procedures
14 TE62 Standard Transfer Tank Operating Instructions 29281927 AB

3 System description
About this chapter
This chapter gives an overview of the Transphor, and a brief description of its function.
Illustration of the instrument
The illustration below shows the main parts of the instrument.
1
2
3
4
5
6
7
3 System description
TE62 Standard Transfer Tank Operating Instructions 29281927 AB 15

Part Function
1 Color-coded leads
2 Safety lid
3 Color-coded electrode panels
4 Cassette hook and holder
5 Tank fill levels
6 Coolant ports (2 ×)
7 Coolant safety valve
Illustration of the transfer stack
assembly
The illustration below shows the main parts of the instrument.
6
1
2
3
4
5
Part Function
1 Sponge (3 mm)
2 Blotting paper
3 Membrane
4 Gel
3 System description
16 TE62 Standard Transfer Tank Operating Instructions 29281927 AB

Part Function
5 Blotting paper
6 Sponge (3 mm) for gels > 1.5 mm
Sponge (6 mm) for gels ≤ 1.5 mm
3 System description
TE62 Standard Transfer Tank Operating Instructions 29281927 AB 17

4 Installation
Introduction
This chapter gives important information that must be considered when unpacking the
product.
Safety precautions
CAUTION
When lifting and moving the instrument be careful not to drop it.
This may cause injury.
CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
Unpacking procedure
Unwrap all packages carefully.
Inspect all visible parts for damage or missing pieces. If any damage is observed, record
this on the receiving documents and inform your Cytiva representative. Make sure to
keep all packing material for damage claims or to use should it become necessary to
return the unit.
4 Installation
18 TE62 Standard Transfer Tank Operating Instructions 29281927 AB

5 Operation
About this chapter
This chapter gives instructions on how to operate the product in a safe way.
Precautions
WARNING
The high voltage power supply must always be disconnected when
the safety lid of the electrophoresis unit is taken off. The high
voltage power supply must never be switched on unless the safety
lid is on the electrophoresis unit.
WARNING
Never exceed the operating limits stated in this document and on
the system label. Operation of the product outside these limits can
damage equipment and cause personal injury or death.
WARNING
Turn all power supply controls off and disconnect the power leads
before removing the safety lid.
WARNING
Rinse only the electrodes (not the banana plugs) with distilled
water before use.
CAUTION
Circulate coolant through the heat exchanger to minimize heating.
Overheating will cause irreparable damage to the unit! Do not
connect the heat exchanger to a water tap or any coolant source
where the water pressure is unregulated.
5 Operation
TE62 Standard Transfer Tank Operating Instructions 29281927 AB 19

CAUTION
Circulate only water or 50/50 water/ethylene glycol through the
heat exchanger. Never use anti-freeze or any organic solvent in the
heat exchanger.
CAUTION
Never introduce anti-freeze or any organic solvent into any part of
the instrument. Organic solvents will cause irreparable damage to
the instrument.
In this chapter
Section See page
5.1 Preparations 21
5.2 Assembly 23
5.3 Electrotransfer 26
5 Operation
20 TE62 Standard Transfer Tank Operating Instructions 29281927 AB