cytiva Ettan IPGphor 3 User manual

Ettan™ IPGphor™ 3
Operating Instructions
Original instructions
cytiva.com

Table of Contents
1Introduction ........................................................................................................ 4
1.1 Important user information ....................................................................................................................... 5
1.2 About this manual ......................................................................................................................................... 6
1.3 Associated documentation ....................................................................................................................... 7
2 Safety instructions ............................................................................................. 8
2.1 Safety precautions ........................................................................................................................................ 9
2.2 Labels ................................................................................................................................................................. 15
2.3 Emergency procedures ............................................................................................................................... 16
3 System description ............................................................................................ 18
3.1 Illustrations of Ettan IPGphor 3 ............................................................................................................... 19
3.2 Ettan IPGphor 3 instrument ....................................................................................................................... 23
3.3 Ettan IPGphor 3 control software ........................................................................................................... 24
3.4 Strip holders ..................................................................................................................................................... 25
4 Installation .......................................................................................................... 27
4.1 Site requirements ......................................................................................................................................... 28
4.2 Unpacking and transport ............................................................................................................................ 30
4.3 Setup ................................................................................................................................................................... 31
5 Operation ............................................................................................................ 36
5.1 Preparations before start ........................................................................................................................... 37
5.1.1 Use the strip holders ................................................................................................................................... 38
5.1.2 Use the Ettan IPGphor Cup Loading Manifold .................................................................................. 49
5.2 Start up the instrument ............................................................................................................................... 54
5.3 Perform a run from the control panel ..................................................................................................... 55
5.3.1 Ettan IPGphor 3 control panel functionality ...................................................................................... 56
5.3.2 Protocol parameters ................................................................................................................................... 59
5.3.3 Set protocol parameters ........................................................................................................................... 60
5.3.4 Isoelectric focusing from the control panel ....................................................................................... 67
5.3.5 Pause or stop a protocol ............................................................................................................................ 72
5.4 Run a pre-programmed protocol from the PC .................................................................................... 73
5.4.1 Setting up a run ............................................................................................................................................. 74
5.4.2 Perform a run ................................................................................................................................................. 79
6 Maintenance ....................................................................................................... 84
6.1 Cleaning before planned service ............................................................................................................. 86
6.2 Cleaning Ettan IPGphor 3 ........................................................................................................................... 87
6.3 Replace fuses .................................................................................................................................................. 90
7 Troubleshooting ................................................................................................. 92
8 Reference information ....................................................................................... 94
8.1 Technical specifications .............................................................................................................................. 95
8.2 Recycling information .................................................................................................................................. 97
8.3 Regulatory information ............................................................................................................................... 98
Table of Contents
Ettan IPGphor 3 Operating Instructions 28963551 AF 2

8.3.1 Contact information .................................................................................................................................... 99
8.3.2 European Union and European Economic Area .............................................................................. 100
8.3.3 Great Britain ................................................................................................................................................... 101
8.3.4 Eurasian Economic Union (Евразийский экономический союз) .......................................... 102
8.3.5 North America ............................................................................................................................................... 104
8.3.6 Regulatory statements .............................................................................................................................. 105
8.3.7 Declaration of Hazardous Substances (DoHS) ................................................................................ 106
8.4 Ordering information ................................................................................................................................... 108
8.5 Health and Safety Declaration Form ...................................................................................................... 109
Index ........................................................................................................................... 111
Table of Contents
Ettan IPGphor 3 Operating Instructions 28963551 AF 3

1 Introduction
About this chapter
This chapter contains information about these Operating Instructions, important user
information, and lists of associated documentation.
In this chapter
Section See page
1.1 Important user information 5
1.2 About this manual 6
1.3 Associated documentation 7
1 Introduction
Ettan IPGphor 3 Operating Instructions 28963551 AF 4

1.1 Important user information
Introduction
This section contains important user information about the product and this manual.
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating, or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not install, operate, or perform maintenance on the product in any other way than
described in the user documentation. If you do, you may be exposed or expose others
to hazards that can lead to personal injury and you may cause damage to the equip-
ment.
Intended use
Ettan™ IPGphor™ 3 Isoelectric Focusing System performs the first-dimension isoelec-
tric focusing (IEF) step in 2-dimensional (2-D) electrophoresis. The system comprises
Cytiva's Ettan IPGphor 3, combined with separate strip holders or a manifold. Two
types of manifolds are available; the ceramic manifold and the light manifold. The IEF
separation protocols are programmed, run and controlled from the Ettan IPGphor 3
control panel or from a PC running the Ettan IPGphor 3 control software.
Ettan IPGphor 3 is intended to be used as a laboratory unit for research purposes and
must only be used by properly trained personnel.
Ettan IPGphor 3 shall not be used in any clinical procedures, or for diagnostic purposes.
For larger sample volumes the Ettan IPGphor 3 Cup Loading Manifold facilitates paper
bridge loading and in gel rehydration loading, for more information see Ettan IPGphor
Cup Loading Manifold User Manual.
1 Introduction
1.1 Important user information
Ettan IPGphor 3 Operating Instructions 28963551 AF 5

1.2 About this manual
Introduction
This section contains information about the purpose and scope of this manual, notes
and tips, and typographical conventions.
Purpose of this manual
This manual provides information needed to install, operate and maintain the product
in a safe way.
Scope of this manual
The document provides you with the instructions needed to use the Ettan IPGphor 3
Isoelectric Focusing System together with Ettan IPGphor 3.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
Text that the user must either type exactly as shown in the manual, or that the software
displays as a response (not a regular part of the graphic user interface), is shown by a
monospaced typeface (for example, Recipe Information).
Tip: The text can include clickable hyperlinks to reference information.
1 Introduction
1.2 About this manual
Ettan IPGphor 3 Operating Instructions 28963551 AF 6

1.3 Associated documentation
Introduction
This section describes associated documentation and how to find related literature
that can be downloaded or ordered from Cytiva.
•Ettan IPGphor Cup Loading Manifold User Manual contains instructions for first-
dimension isoelectric focusing of proteins on IPG strips.
•2-D Electrophoresis, principles and methods guide
•Instructions attached to each package of Immobiline™ DryStrip.
1 Introduction
1.3 Associated documentation
Ettan IPGphor 3 Operating Instructions 28963551 AF 7

2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
instrument. In addition, the chapter describes emergency and recovery procedures.
In this chapter
Section See page
2.1 Safety precautions 9
2.2 Labels 15
2.3 Emergency procedures 16
Important
WARNING
All users must read and understand the entire contents of
this general safety chapter, and the specific safety precau-
tions information in each subsequent chapter of this manual
to become aware of the hazards involved.
2 Safety instructions
Ettan IPGphor 3 Operating Instructions 28963551 AF 8

2.1 Safety precautions
Introduction
Before operating the instrument, you must be aware of the hazards described in the
user documentation. Follow the instructions provided to avoid personal injury or
damage to the equipment.
The safety precautions in this section are grouped into the following categories:
•General precautions, on page 10
•Personal protection, on page 10
•Using flammable liquids, on page 11
•Maintenance, on page 14
•System operation, on page 12
•Maintenance, on page 14
Definitions
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
2 Safety instructions
2.1 Safety precautions
Ettan IPGphor 3 Operating Instructions 28963551 AF 9

General precautions
WARNING
Risk assessment. Perform a risk assessment for the process or
process environment. Evaluate the effects the use of the product
and the operational processes may have on the classification of the
hazardous area. The process can cause the hazardous area to
increase or the zone classification to change. Implement the risk
reduction measures needed, including use of personal protective
equipment.
WARNING
The customer must make sure that all installation, maintenance,
operation and inspection is carried out by qualified personnel who
are adequately trained, understand and adhere to local regulations
and the operating instructions, and have a thorough knowledge of
the product and the entire process.
WARNING
Do not operate the product in any other way than as described in
the user documentation.
WARNING
Only properly trained personnel are allowed to operate and main-
tain the product.
WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by Cytiva may be used for maintaining or
servicing the product.
Personal protection
WARNING
Always use appropriate Personal Protective Equipment (PPE)
during operation and maintenance of this product.
2 Safety instructions
2.1 Safety precautions
Ettan IPGphor 3 Operating Instructions 28963551 AF 10