cytiva Ettan IPGphor 3 User manual

Ettan™ IPGphor™ 3
Operating Instructions
Original instructions
cytiva.com

Table of Contents
1Introduction ........................................................................................................ 4
1.1 Important user information ....................................................................................................................... 5
1.2 About this manual ......................................................................................................................................... 6
1.3 Associated documentation ....................................................................................................................... 7
2 Safety instructions ............................................................................................. 8
2.1 Safety precautions ........................................................................................................................................ 9
2.2 Labels ................................................................................................................................................................. 15
2.3 Emergency procedures ............................................................................................................................... 16
3 System description ............................................................................................ 18
3.1 Illustrations of Ettan IPGphor 3 ............................................................................................................... 19
3.2 Ettan IPGphor 3 instrument ....................................................................................................................... 23
3.3 Ettan IPGphor 3 control software ........................................................................................................... 24
3.4 Strip holders ..................................................................................................................................................... 25
4 Installation .......................................................................................................... 27
4.1 Site requirements ......................................................................................................................................... 28
4.2 Unpacking and transport ............................................................................................................................ 30
4.3 Setup ................................................................................................................................................................... 31
5 Operation ............................................................................................................ 36
5.1 Preparations before start ........................................................................................................................... 37
5.1.1 Use the strip holders ................................................................................................................................... 38
5.1.2 Use the Ettan IPGphor Cup Loading Manifold .................................................................................. 49
5.2 Start up the instrument ............................................................................................................................... 54
5.3 Perform a run from the control panel ..................................................................................................... 55
5.3.1 Ettan IPGphor 3 control panel functionality ...................................................................................... 56
5.3.2 Protocol parameters ................................................................................................................................... 59
5.3.3 Set protocol parameters ........................................................................................................................... 60
5.3.4 Isoelectric focusing from the control panel ....................................................................................... 67
5.3.5 Pause or stop a protocol ............................................................................................................................ 72
5.4 Run a pre-programmed protocol from the PC .................................................................................... 73
5.4.1 Setting up a run ............................................................................................................................................. 74
5.4.2 Perform a run ................................................................................................................................................. 79
6 Maintenance ....................................................................................................... 84
6.1 Cleaning before planned service ............................................................................................................. 86
6.2 Cleaning Ettan IPGphor 3 ........................................................................................................................... 87
6.3 Replace fuses .................................................................................................................................................. 90
7 Troubleshooting ................................................................................................. 92
8 Reference information ....................................................................................... 94
8.1 Technical specifications .............................................................................................................................. 95
8.2 Recycling information .................................................................................................................................. 97
8.3 Regulatory information ............................................................................................................................... 98
Table of Contents
Ettan IPGphor 3 Operating Instructions 28963551 AF 2

8.3.1 Contact information .................................................................................................................................... 99
8.3.2 European Union and European Economic Area .............................................................................. 100
8.3.3 Great Britain ................................................................................................................................................... 101
8.3.4 Eurasian Economic Union (Евразийский экономический союз) .......................................... 102
8.3.5 North America ............................................................................................................................................... 104
8.3.6 Regulatory statements .............................................................................................................................. 105
8.3.7 Declaration of Hazardous Substances (DoHS) ................................................................................ 106
8.4 Ordering information ................................................................................................................................... 108
8.5 Health and Safety Declaration Form ...................................................................................................... 109
Index ........................................................................................................................... 111
Table of Contents
Ettan IPGphor 3 Operating Instructions 28963551 AF 3

1 Introduction
About this chapter
This chapter contains information about these Operating Instructions, important user
information, and lists of associated documentation.
In this chapter
Section See page
1.1 Important user information 5
1.2 About this manual 6
1.3 Associated documentation 7
1 Introduction
Ettan IPGphor 3 Operating Instructions 28963551 AF 4

1.1 Important user information
Introduction
This section contains important user information about the product and this manual.
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating, or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not install, operate, or perform maintenance on the product in any other way than
described in the user documentation. If you do, you may be exposed or expose others
to hazards that can lead to personal injury and you may cause damage to the equip-
ment.
Intended use
Ettan™ IPGphor™ 3 Isoelectric Focusing System performs the first-dimension isoelec-
tric focusing (IEF) step in 2-dimensional (2-D) electrophoresis. The system comprises
Cytiva's Ettan IPGphor 3, combined with separate strip holders or a manifold. Two
types of manifolds are available; the ceramic manifold and the light manifold. The IEF
separation protocols are programmed, run and controlled from the Ettan IPGphor 3
control panel or from a PC running the Ettan IPGphor 3 control software.
Ettan IPGphor 3 is intended to be used as a laboratory unit for research purposes and
must only be used by properly trained personnel.
Ettan IPGphor 3 shall not be used in any clinical procedures, or for diagnostic purposes.
For larger sample volumes the Ettan IPGphor 3 Cup Loading Manifold facilitates paper
bridge loading and in gel rehydration loading, for more information see Ettan IPGphor
Cup Loading Manifold User Manual.
1 Introduction
1.1 Important user information
Ettan IPGphor 3 Operating Instructions 28963551 AF 5

1.2 About this manual
Introduction
This section contains information about the purpose and scope of this manual, notes
and tips, and typographical conventions.
Purpose of this manual
This manual provides information needed to install, operate and maintain the product
in a safe way.
Scope of this manual
The document provides you with the instructions needed to use the Ettan IPGphor 3
Isoelectric Focusing System together with Ettan IPGphor 3.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
Text that the user must either type exactly as shown in the manual, or that the software
displays as a response (not a regular part of the graphic user interface), is shown by a
monospaced typeface (for example, Recipe Information).
Tip: The text can include clickable hyperlinks to reference information.
1 Introduction
1.2 About this manual
Ettan IPGphor 3 Operating Instructions 28963551 AF 6

1.3 Associated documentation
Introduction
This section describes associated documentation and how to find related literature
that can be downloaded or ordered from Cytiva.
•Ettan IPGphor Cup Loading Manifold User Manual contains instructions for first-
dimension isoelectric focusing of proteins on IPG strips.
•2-D Electrophoresis, principles and methods guide
•Instructions attached to each package of Immobiline™ DryStrip.
1 Introduction
1.3 Associated documentation
Ettan IPGphor 3 Operating Instructions 28963551 AF 7

2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
instrument. In addition, the chapter describes emergency and recovery procedures.
In this chapter
Section See page
2.1 Safety precautions 9
2.2 Labels 15
2.3 Emergency procedures 16
Important
WARNING
All users must read and understand the entire contents of
this general safety chapter, and the specific safety precau-
tions information in each subsequent chapter of this manual
to become aware of the hazards involved.
2 Safety instructions
Ettan IPGphor 3 Operating Instructions 28963551 AF 8

2.1 Safety precautions
Introduction
Before operating the instrument, you must be aware of the hazards described in the
user documentation. Follow the instructions provided to avoid personal injury or
damage to the equipment.
The safety precautions in this section are grouped into the following categories:
•General precautions, on page 10
•Personal protection, on page 10
•Using flammable liquids, on page 11
•Maintenance, on page 14
•System operation, on page 12
•Maintenance, on page 14
Definitions
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
2 Safety instructions
2.1 Safety precautions
Ettan IPGphor 3 Operating Instructions 28963551 AF 9

General precautions
WARNING
Risk assessment. Perform a risk assessment for the process or
process environment. Evaluate the effects the use of the product
and the operational processes may have on the classification of the
hazardous area. The process can cause the hazardous area to
increase or the zone classification to change. Implement the risk
reduction measures needed, including use of personal protective
equipment.
WARNING
The customer must make sure that all installation, maintenance,
operation and inspection is carried out by qualified personnel who
are adequately trained, understand and adhere to local regulations
and the operating instructions, and have a thorough knowledge of
the product and the entire process.
WARNING
Do not operate the product in any other way than as described in
the user documentation.
WARNING
Only properly trained personnel are allowed to operate and main-
tain the product.
WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by Cytiva may be used for maintaining or
servicing the product.
Personal protection
WARNING
Always use appropriate Personal Protective Equipment (PPE)
during operation and maintenance of this product.
2 Safety instructions
2.1 Safety precautions
Ettan IPGphor 3 Operating Instructions 28963551 AF 10

WARNING
Hazardous substances and biological agents. When using
hazardous chemical or biological agents, take all suitable protec-
tive measures, such as wearing protective clothing, glasses and
gloves resistant to the substances used. Follow local and national
regulations for safe operation and maintenance of this product.
WARNING
Spread of biological agents. The operator must take all neces-
sary actions to avoid spreading hazardous biological agents. The
facility must comply with the national code of practice for
biosafety.
Using flammable liquids
WARNING
Fire Hazard. Before starting the system, make sure that there is no
leakage.
WARNING
Ventilation system. A fume hood or similar ventilation system
must be used when working with flammable or noxious
substances.
Installation
WARNING
Supply voltage. Before connecting the power cord, make sure
that the supply voltage at the wall outlet corresponds to the
requirements for the instrument.
WARNING
Protective ground. The instrument must be connected to a
grounded power outlet.
2 Safety instructions
2.1 Safety precautions
Ettan IPGphor 3 Operating Instructions 28963551 AF 11

WARNING
Power cord. Only use power cords with plugs delivered or
approved by Cytiva.
WARNING
Access to power switch and power cord. The power switch
must always be easy to access. The power cord must always be
easy to disconnect.
NOTICE
Any computer used with the equipment must comply with
IEC 60950 or IEC 62368-1 and be installed and used according to
the manufacturer's instructions.
System operation
WARNING
Hazardous voltage. Make sure that the high voltage is turned off
before opening the safety lid. The HV ON indicator on the front
panel should not light.
WARNING
Do not use the product if it is not working properly, or if it has
suffered any damage including:
•damage to the power cord or its plug,
•damage caused by dropping the product,
•damage caused by splashing liquid onto the product.
WARNING
The instrument must never be used if any safety functions are out
of order. Contact your Cytiva service operator for more informa-
tion.
2 Safety instructions
2.1 Safety precautions
Ettan IPGphor 3 Operating Instructions 28963551 AF 12

WARNING
Ettan IPGphor 3 is a high voltage instrument that can cause fatal
electrical shock if the safety features are disabled. The safety lid
must be securely closed before starting a protocol.
WARNING
In a situation where there is a risk of injury, turn off the instrument
by switching the mains power switch (located on the rear panel) to
position 0. Malfunctions must be rectified before Ettan IPGphor 3
Isoelectric Focusing System is restarted.
WARNING
The vents at the front, back, and bottom of Ettan IPGphor 3 must
not be obstructed. Air must be free to circulate for the cooling
apparatus to function correctly. There should be a minimum of 10
cm unobstructed space behind the rear panel of the instrument.
WARNING
Avoid spilling liquids on the body of the instrument. If large volumes
of liquid have penetrated the casing of the instrument and come
into contact with the electrical components, switch off the instru-
ment immediately and contact an authorized service technician.
CAUTION
Always wear protective gloves when working with rehydration
solution.
CAUTION
Always wear gloves when handling IPG strips and the equipment
that comes in contact with them. This will help minimize protein
contamination which can result in artifactual spots in stained 2-D
gel patterns.
2 Safety instructions
2.1 Safety precautions
Ettan IPGphor 3 Operating Instructions 28963551 AF 13

CAUTION
The safety lid must be properly closed before power is applied.
Maintenance
WARNING
Disconnect power. Always disconnect power from the instru-
ment before performing any maintenance task.
WARNING
Ettan IPGphor 3 covers must not be opened by the user. It contains
electrical circuits which can give a lethal electric shock. Service and
planned maintenance should be performed by personnel author-
ized by Cytiva.
WARNING
Do not remove the main cover. There are no user serviceable
components inside, and you can be exposed to high voltage.
WARNING
Disconnect power. The power must be disconnected before
replacing fuses.
CAUTION
Do not use any organic solvents during cleaning.
2 Safety instructions
2.1 Safety precautions
Ettan IPGphor 3 Operating Instructions 28963551 AF 14

2.2 Labels
Introduction
This section describes the system label and the safety labels on Ettan IPGphor 3.
System label
The system label is located on the back of the equipment. The system label identifies
the equipment and shows electrical data, regulatory compliance, and warning
symbols.
System label description
Label Description
Code no. Instrument Assembly Number
Serial no. Instrument serial number
Mfg Year Manufacturing year and month
Voltage Voltage
Frequency Frequency
Max Power Max Power rating
Fuse Fuse
Warning! Read the user documentation before using the
system. Do not open any covers or replace parts unless
specifically stated in the user documentation.
Safety label description
Safety labels (see figure below) are attached to both the light protective cover and the
transparent lid of the safety lid. The safety label warns the user of risk for personal
injury. Do not proceed until the instructions are clearly understood and all stated
conditions are met.
2 Safety instructions
2.2 Labels
Ettan IPGphor 3 Operating Instructions 28963551 AF 15

2.3 Emergency procedures
Introduction
This section describes how to perform an emergency shutdown of Ettan IPGphor 3 and
the result in the event of power failure.
Emergency shutdown
Switch off power to the instrument by pressing the Power switch to the 0 position (1). If
required, disconnect the mains power cord (2). The run is interrupted immediately.
This will ensure that the power is cut immediately even if an uninterruptible power
supply (UPS) is used.
1
2
Power failure
In the event of system shutdown due to power failure, emergency stop or process
interruption, malfunctions must be rectified before Ettan IPGphor 3 is restarted.
The result of a power failure depends on which unit is affected.
Power failure to... will result in...
Ettan IPGphor 3 instru-
ment
•Any experiments that are running will stop.
•The time for the stop will be logged in the instrument.
•The run is interrupted in an undefined state.
Computer •The Ettan IPGphor computer shuts down.
•Any running experiments will be continued and
completed.
•Scanned images will be saved and uploaded to the
computer when computer contact has been re-
established.
Note: A UPS can eliminate data loss during and after a power failure, and allow
time for a controlled shut-down of Ettan IPGphor 3.
2 Safety instructions
2.3 Emergency procedures
Ettan IPGphor 3 Operating Instructions 28963551 AF 16

Restart after emergency shutdown/
power failure
When the power returns to normal no experiments that were running at the time of
emergency shutdown will be continued. Proceed as follows:
Step Action
1Start the instrument, see Turn on power and run diagnostic program, on
page 54.
2Restart the software.
When the system is connected, the log event will be stored in the system log.
3If you wish to continue a run, set up and run a new experiment with the
appropriate/remaining steps to complete the run in the best way.
2 Safety instructions
2.3 Emergency procedures
Ettan IPGphor 3 Operating Instructions 28963551 AF 17

3 System description
About this chapter
This section provides a description of Ettan IPGphor 3 Isoelectric Focusing System and
an overview of its components.
In this chapter
Section See page
3.1 Illustrations of Ettan IPGphor 3 19
3.2 Ettan IPGphor 3 instrument 23
3.3 Ettan IPGphor 3 control software 24
3.4 Strip holders 25
3 System description
Ettan IPGphor 3 Operating Instructions 28963551 AF 18

3.1 Illustrations of Ettan IPGphor 3
Ettan IPGphor 3
Isoelectric Focusing System
1
2
Part Function
1 Ettan IPGphor 3 control software
2 Ettan IPGphor 3 instrument
Note: Ettan IPGphor 3 instrument is used together with separate strip holders or a
manifold. Two types of manifolds are available; the ceramic manifold and
the light manifold.
Ettan IPGphor 3 Isoelectric Focusing System also includes Immobiline
DryStrip gel strips, which contain an immobilized pH gradient (IPG).
For more information about the manifolds, see Ettan IPGphor Cup Loading
Manifold User Manual.
3 System description
3.1 Illustrations of Ettan IPGphor 3
Ettan IPGphor 3 Operating Instructions 28963551 AF 19

Ettan IPGphor 3 platform
1
2
3
4
Part Function
1 Safety lid
2 Positive (anode) electrode area
3 Negative (cathode) electrode area
4 Control panel
3 System description
3.1 Illustrations of Ettan IPGphor 3
Ettan IPGphor 3 Operating Instructions 28963551 AF 20