cytiva Monitor UVis-920 User manual

Monitor UVis-920
Operating Instructions
Original instructions
Translation disc included
cytiva.com

Page intentionally left blank

Table of Contents
1 Introduction ........................................................................................................ 5
1.1 About this manual ......................................................................................................................................... 6
1.2 Important user information ....................................................................................................................... 7
1.3 Associated documentation ....................................................................................................................... 9
2 Safety instructions ............................................................................................. 10
2.1 Safety precautions ........................................................................................................................................ 11
2.2 Labels ................................................................................................................................................................. 15
2.3 Emergency procedures ............................................................................................................................... 16
3 Instrument description ...................................................................................... 17
3.1 Instrument overview .................................................................................................................................... 18
3.2 Monitor principle ............................................................................................................................................ 21
3.3 Flow cell principle ........................................................................................................................................... 23
4 Installation .......................................................................................................... 24
4.1 Site requirements .......................................................................................................................................... 25
4.2 Unpacking ......................................................................................................................................................... 26
4.3 Transport .......................................................................................................................................................... 27
4.4 Installing the cell holder .............................................................................................................................. 28
4.5 Installing the flow cells ................................................................................................................................. 30
4.5.1 UV Flow Cell 2 mm and UV Flow Cell 10 mm ..................................................................................... 31
4.5.2 UV Flow Cell ÄKTApilot 1/2/5 mm and Industrial Flow Cell 8 mm and 1 inch ..................... 37
4.6 Electrical connections ................................................................................................................................. 39
5 Operation ............................................................................................................ 42
5.1 Menu selection ............................................................................................................................................... 43
5.2 Starting the instrument ............................................................................................................................... 46
5.3 Main menu overview ..................................................................................................................................... 47
5.4 Setting wavelength ....................................................................................................................................... 48
5.5 Custom filters .................................................................................................................................................. 49
5.6 Autozero ............................................................................................................................................................ 50
5.7 Reading absorbance values ....................................................................................................................... 51
5.8 Setup menu ...................................................................................................................................................... 52
5.9 Check menu ..................................................................................................................................................... 57
5.10 UV cell calibration .......................................................................................................................................... 60
5.11 Changing flow cell .......................................................................................................................................... 61
6 Maintenance ....................................................................................................... 62
6.1 Cleaning before planned service ............................................................................................................. 63
6.2 Cleaning the instrument housing ............................................................................................................ 64
6.3 Cleaning the flow cell and optical connectors .................................................................................... 65
6.4 Storage .............................................................................................................................................................. 67
7 Troubleshooting ................................................................................................. 68
7.1 Problems and corrective actions ............................................................................................................. 69
Table of Contents
Monitor UVis-920 Operating Instructions 29055049 AF 3

7.2 Error messages ............................................................................................................................................... 71
8 Reference information ....................................................................................... 72
8.1 Specifications .................................................................................................................................................. 73
8.2 Recycling information .................................................................................................................................. 78
8.3 Regulatory information ............................................................................................................................... 79
8.3.1 Contact information .................................................................................................................................... 80
8.3.2 European Union and European Economic Area .............................................................................. 81
8.3.3 Eurasian Economic Union
Евразийский экономический союз .................................................................................................. 82
8.3.4 Regulations for North America ............................................................................................................... 84
8.3.5 Regulatory statements .............................................................................................................................. 85
8.3.6 Declaration of Hazardous Substances (DoHS) ................................................................................ 86
8.4 Ordering information ................................................................................................................................... 88
8.5 Health and Safety Declaration Form ...................................................................................................... 91
Table of Contents
4 Monitor UVis-920 Operating Instructions 29055049 AF

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, regu-
latory information, intended use of the Monitor UVis-920 instrument, and lists of asso-
ciated documentation.
In this chapter
Section See page
1.1 About this manual 6
1.2 Important user information 7
1.3 Associated documentation 9
1 Introduction
Monitor UVis-920 Operating Instructions 29055049 AF 5

1.1 About this manual
Purpose of this manual
The Operating Instructions provide you with the information needed to install, operate
and maintain the product in a safe way.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
In electronic format, references in italics are clickable hyperlinks.
1 Introduction
1.1 About this manual
6 Monitor UVis-920 Operating Instructions 29055049 AF

1.2 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documenta-
tion. If you do, you may be exposed to hazards that can lead to personal injury and you
may cause damage to the equipment.
Intended use of the product
Monitor UVis-920 is a UV and visible light absorption monitor for use in liquid chroma-
tography.
Monitor UVis-920 is intended for research use only, and shall not be used in any clinical
procedures, or for diagnostic purposes.
Prerequisites
In order to operate Monitor UVis-920 in the way it is intended:
•The user must be acquainted with the use of bioprocessing equipment and with the
handling of biological materials.
•The user must read and understand the Safety instructions chapter in the Operating
Instructions.
•The system must be installed according to the instructions in the Installation
chapter of the Operating Instructions.
Safety notices
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
1 Introduction
1.2 Important user information
Monitor UVis-920 Operating Instructions 29055049 AF 7

CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
1 Introduction
1.2 Important user information
8 Monitor UVis-920 Operating Instructions 29055049 AF

1.3 Associated documentation
Introduction
This section describes the user documentation that is delivered with the product.
System-specific documentation
In addition to the Operating Instructions manual, the documentation package supplied
with Monitor UVis-920 also includes one or more Product Documentation binders
containing detailed specifications and traceability documents.
The most important documents in the documentation package, with regard to tech-
nical aspects of Monitor UVis-920, are listed in the table below.
Documentation Main contents
Monitor UVis-920 Operating Instruc-
tions
Instructions needed to install, operate
and maintain Monitor UVis-920 in a safe
way.
Declaration of Conformity Declaration of Conformity for EU and/or
other regions.
1 Introduction
1.3 Associated documentation
Monitor UVis-920 Operating Instructions 29055049 AF 9

2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition, the chapter describes emergency and recovery procedures,
and provides recycling information.
In this chapter
Section See page
2.1 Safety precautions 11
2.2 Labels 15
2.3 Emergency procedures 16
2 Safety instructions
10 Monitor UVis-920 Operating Instructions 29055049 AF