cytiva Monitor UVis-920 User manual

Monitor UVis-920
Operating Instructions
Original instructions
Translation disc included
cytiva.com

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Table of Contents
1 Introduction ........................................................................................................ 5
1.1 About this manual ......................................................................................................................................... 6
1.2 Important user information ....................................................................................................................... 7
1.3 Associated documentation ....................................................................................................................... 9
2 Safety instructions ............................................................................................. 10
2.1 Safety precautions ........................................................................................................................................ 11
2.2 Labels ................................................................................................................................................................. 15
2.3 Emergency procedures ............................................................................................................................... 16
3 Instrument description ...................................................................................... 17
3.1 Instrument overview .................................................................................................................................... 18
3.2 Monitor principle ............................................................................................................................................ 21
3.3 Flow cell principle ........................................................................................................................................... 23
4 Installation .......................................................................................................... 24
4.1 Site requirements .......................................................................................................................................... 25
4.2 Unpacking ......................................................................................................................................................... 26
4.3 Transport .......................................................................................................................................................... 27
4.4 Installing the cell holder .............................................................................................................................. 28
4.5 Installing the flow cells ................................................................................................................................. 30
4.5.1 UV Flow Cell 2 mm and UV Flow Cell 10 mm ..................................................................................... 31
4.5.2 UV Flow Cell ÄKTApilot 1/2/5 mm and Industrial Flow Cell 8 mm and 1 inch ..................... 37
4.6 Electrical connections ................................................................................................................................. 39
5 Operation ............................................................................................................ 42
5.1 Menu selection ............................................................................................................................................... 43
5.2 Starting the instrument ............................................................................................................................... 46
5.3 Main menu overview ..................................................................................................................................... 47
5.4 Setting wavelength ....................................................................................................................................... 48
5.5 Custom filters .................................................................................................................................................. 49
5.6 Autozero ............................................................................................................................................................ 50
5.7 Reading absorbance values ....................................................................................................................... 51
5.8 Setup menu ...................................................................................................................................................... 52
5.9 Check menu ..................................................................................................................................................... 57
5.10 UV cell calibration .......................................................................................................................................... 60
5.11 Changing flow cell .......................................................................................................................................... 61
6 Maintenance ....................................................................................................... 62
6.1 Cleaning before planned service ............................................................................................................. 63
6.2 Cleaning the instrument housing ............................................................................................................ 64
6.3 Cleaning the flow cell and optical connectors .................................................................................... 65
6.4 Storage .............................................................................................................................................................. 67
7 Troubleshooting ................................................................................................. 68
7.1 Problems and corrective actions ............................................................................................................. 69
Table of Contents
Monitor UVis-920 Operating Instructions 29055049 AF 3

7.2 Error messages ............................................................................................................................................... 71
8 Reference information ....................................................................................... 72
8.1 Specifications .................................................................................................................................................. 73
8.2 Recycling information .................................................................................................................................. 78
8.3 Regulatory information ............................................................................................................................... 79
8.3.1 Contact information .................................................................................................................................... 80
8.3.2 European Union and European Economic Area .............................................................................. 81
8.3.3 Eurasian Economic Union
Евразийский экономический союз .................................................................................................. 82
8.3.4 Regulations for North America ............................................................................................................... 84
8.3.5 Regulatory statements .............................................................................................................................. 85
8.3.6 Declaration of Hazardous Substances (DoHS) ................................................................................ 86
8.4 Ordering information ................................................................................................................................... 88
8.5 Health and Safety Declaration Form ...................................................................................................... 91
Table of Contents
4 Monitor UVis-920 Operating Instructions 29055049 AF

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, regu-
latory information, intended use of the Monitor UVis-920 instrument, and lists of asso-
ciated documentation.
In this chapter
Section See page
1.1 About this manual 6
1.2 Important user information 7
1.3 Associated documentation 9
1 Introduction
Monitor UVis-920 Operating Instructions 29055049 AF 5

1.1 About this manual
Purpose of this manual
The Operating Instructions provide you with the information needed to install, operate
and maintain the product in a safe way.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
In electronic format, references in italics are clickable hyperlinks.
1 Introduction
1.1 About this manual
6 Monitor UVis-920 Operating Instructions 29055049 AF

1.2 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documenta-
tion. If you do, you may be exposed to hazards that can lead to personal injury and you
may cause damage to the equipment.
Intended use of the product
Monitor UVis-920 is a UV and visible light absorption monitor for use in liquid chroma-
tography.
Monitor UVis-920 is intended for research use only, and shall not be used in any clinical
procedures, or for diagnostic purposes.
Prerequisites
In order to operate Monitor UVis-920 in the way it is intended:
•The user must be acquainted with the use of bioprocessing equipment and with the
handling of biological materials.
•The user must read and understand the Safety instructions chapter in the Operating
Instructions.
•The system must be installed according to the instructions in the Installation
chapter of the Operating Instructions.
Safety notices
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
1 Introduction
1.2 Important user information
Monitor UVis-920 Operating Instructions 29055049 AF 7

CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
1 Introduction
1.2 Important user information
8 Monitor UVis-920 Operating Instructions 29055049 AF

1.3 Associated documentation
Introduction
This section describes the user documentation that is delivered with the product.
System-specific documentation
In addition to the Operating Instructions manual, the documentation package supplied
with Monitor UVis-920 also includes one or more Product Documentation binders
containing detailed specifications and traceability documents.
The most important documents in the documentation package, with regard to tech-
nical aspects of Monitor UVis-920, are listed in the table below.
Documentation Main contents
Monitor UVis-920 Operating Instruc-
tions
Instructions needed to install, operate
and maintain Monitor UVis-920 in a safe
way.
Declaration of Conformity Declaration of Conformity for EU and/or
other regions.
1 Introduction
1.3 Associated documentation
Monitor UVis-920 Operating Instructions 29055049 AF 9

2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition, the chapter describes emergency and recovery procedures,
and provides recycling information.
In this chapter
Section See page
2.1 Safety precautions 11
2.2 Labels 15
2.3 Emergency procedures 16
2 Safety instructions
10 Monitor UVis-920 Operating Instructions 29055049 AF

2.1 Safety precautions
Introduction
Monitor UVis-920 is powered by mains voltage and handles materials that can be
hazardous. Before installing, operating or maintaining the system, you must be aware
of the hazards described in this manual.
Follow the instructions to avoid injury to the operator or other personnel,
damage to samples or other substances handled by the equipment, to the
product, or to other equipment in the area.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Personal protection
•Installing and moving the product
•Operation
•Maintenance
General precautions
WARNING
Do not operate the product in any other way than described in the
user documentation.
WARNING
Only properly trained personnel may operate and maintain the
product.
Personal protection
WARNING
Always use appropriate Personal Protective Equipment (PPE)
during operation and maintenance of this product.
2 Safety instructions
2.1 Safety precautions
Monitor UVis-920 Operating Instructions 29055049 AF 11

WARNING
Hazardous substances and biological agents. When using
hazardous chemical and biological agents, take all suitable protec-
tive measures, such as wearing protective clothing, glasses and
gloves resistant to the substances used. Follow local and/or
national regulations for safe operation and maintenance of this
product.
WARNING
Spread of biological agents. The operator must take all neces-
sary actions to avoid spreading hazardous biological agents. The
facility must comply with the national code of practice for
biosafety.
Installing and moving the product
WARNING
High intensity UV light. This product uses high intensity ultra-
violet light. Do not disconnect the optical fibers while the lamp is
on.
WARNING
Protective ground. The product must always be connected to a
grounded power outlet.
WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by Cytiva.
WARNING
Access to power switch and power cord with plug. Do not
block access to the power switch and power cord. The power switch
must always be easy to access. The power cord with plug must
always be easy to disconnect.
2 Safety instructions
2.1 Safety precautions
12 Monitor UVis-920 Operating Instructions 29055049 AF

WARNING
If an external computer is used, the computer must be installed
and used according to the instructions provided by the manufac-
turer of the computer. In particular regarding additional precau-
tions required in the presence of moisture or liquids.
CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
Operation
WARNING
If liquid is spilled on the equipment, the electrical power supply
must be disconnected immediately. The equipment must be
completely dry on the inside and the outside before reconnecting
the power supply.
Maintenance
WARNING
Electrical shock hazard. All repairs should be done by service
personnel authorized by Cytiva. Do not open any covers or replace
parts unless specifically stated in the user documentation.
WARNING
Disconnect power. Always disconnect power from the instru-
ment before performing any maintenance task.
WARNING
Hazardous chemicals or biological agents. When using
hazardous chemicals or biological agents, make sure that the
entire system has been flushed thoroughly with bacteriostatic
solution (e.g., NaOH) and distilled water before service and mainte-
nance.
2 Safety instructions
2.1 Safety precautions
Monitor UVis-920 Operating Instructions 29055049 AF 13

WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by Cytiva may be used for maintaining or
servicing the product.
WARNING
Corrosive substance. NaOH is corrosive and therefore dangerous
to health. When using hazardous chemicals, avoid spillage and
wear protective glasses and other suitable Personal Protective
Equipment (PPE).
CAUTION
Hazardous chemicals or biological agents in UV flow cell.
Make sure that the entire flow cell has been flushed thoroughly
with bacteriostatic solution (e.g., NaOH) and distilled water, before
service and maintenance.
2 Safety instructions
2.1 Safety precautions
14 Monitor UVis-920 Operating Instructions 29055049 AF

2.2 Labels
Introduction
This section describes the product nameplate (label) and other safety and regulatory
labels attached to the product.
System label
The system label is located on the back of the equipment. The system label identifies
the equipment and shows electrical data, regulatory compliance, and warning
symbols.
Description of symbols on the system
label
The following symbols and text may be present on the system label:
Symbol/text Meaning
Warning! Read the user documentation before using the system.
Do not open any covers or replace parts unless specifically stated
in the user documentation.
CAN
ICES-1/
NMB-1
CAN ICES-1/NMB-1 indicates that this product complies with the
Canadian standard ICES-001 concerning technical requirements
relative to radiated noise emissions from Industrial, Scientific and
Medical radio frequency generators.
Code no Instrument assembly number
Serial no Instrument serial number
Mfg Year Year (YYYY) and month (MM) of manufacture
Voltage
Frequency
Max Power
Electrical requirements:
•Voltage (VAC )
•Frequency (Hz)
•Max. power (VA)
2 Safety instructions
2.2 Labels
Monitor UVis-920 Operating Instructions 29055049 AF 15

2.3 Emergency procedures
Introduction
This section describes how to shut down the Monitor UVis-920 instrument in an emer-
gency situation, and the procedure for restarting the Monitor UVis-920 instrument.
The section also describes the result in the event of power failure.
Emergency shutdown
In an emergency situation, follow the steps below to stop the run:
Step Action
1Switch off power to the instrument by switching the On/off switch on the
rear panel to the (O) position.
Result:
The run is interrupted immediately.
2Disconnect the mains power cord.
Power failure
In the event of power failure the run is interrupted immediately.
Restart after power failure
If the power supply to the instrument is interrupted, the instrument automatically
restarts itself and displays the main operating menu. All set values are retained in the
instrument but the instrument starts with the lamp switched off (standby).
2 Safety instructions
2.3 Emergency procedures
16 Monitor UVis-920 Operating Instructions 29055049 AF

3 Instrument description
About this chapter
This chapter gives an overview of the Monitor UVis-920 instrument, and a brief descrip-
tion of its function.
In this chapter
Section See page
3.1 Instrument overview 18
3.2 Monitor principle 21
3.3 Flow cell principle 23
3 Instrument description
Monitor UVis-920 Operating Instructions 29055049 AF 17

3.1 Instrument overview
Introduction to Monitor UVis-920
The Monitor UVis-920 is a UV and visible light absorption monitor which can be used in
wavelengths range between 200-700 nm by changeable fixed wavelength filters. The
instrument contains no internal user replaceable items.
Illustration of the instrument
The illustration below shows the Monitor UVis-920 instrument.
12
Part Function
1 Main unit
2 UV cell holder with cover
3 Instrument description
3.1 Instrument overview
18 Monitor UVis-920 Operating Instructions 29055049 AF

Illustration of the front panel
The illustration below shows the front panel of Monitor UVis-920.
1
2
3
4
5 6 7 8
9
10
Part Function
1 Eject button for filter unit
2OK key
3run/end key
4 Display
5 Arrow up key
6 Arrow down key
7event mark key
8autozero key
9Esc key
10 Filter unit insert
3 Instrument description
3.1 Instrument overview
Monitor UVis-920 Operating Instructions 29055049 AF 19

Illustration of the rear panel
The illustration below shows the rear panel of Monitor UVis-920.
Voltage 100 - 240 V
Frequency 50 - 60 Hz
Power max 35 VA
1
2
3
4
5
Part Function
1 Analog out 0-1 V. Recorder output, 1 channel 0 V to 1 V
2 For service use only
3 Remote. Digital control signals.
4 Instrument on/off switch
5 Mains. Supply voltage, grounded.
3 Instrument description
3.1 Instrument overview
20 Monitor UVis-920 Operating Instructions 29055049 AF