Endress+Hauser analytikjena SPECORD PLUS User manual

Operating Manual
SPECORD PLUS
UV/Vis Spectrophotometer

Manufacturer Analytik Jena GmbH
Konrad-Zuse-Strasse 1
07745 Jena / Germany
Phone: [phone]
Fax: [phone]
Email: [email protected]
Technical Service AnalytikJenaGmbH
Konrad-Zuse-Strasse 1
07745 Jena / Germany
Phone: [phone]
Fax: [phone]
Email: [email protected]
General Information http://www.analytik-jena.com
Documentation Number 10-2210-012-23
Edition B (11/2021)
Technical Documentation Analytik Jena GmbH
© Copyright 2021, Analytik Jena GmbH
For a proper and safe use of this product follow the instructions. Keep the
operating manual for future reference.

SPECORD PLUS Table of contents
3
Table of contents
1 About this operating manual ......................................................................................................................................... 5
2 Intended use of the SPECORD PLUS.............................................................................................................................. 6
3 Security............................................................................................................................................................................ 7
3.1 Safety labeling on the device ........................................................................................................................... 7
3.2 Requirements for the operating personnel..................................................................................................... 8
3.3 Safety instruction–explosion protection........................................................................................................ 8
3.4 Safety instructions: during operation .............................................................................................................. 8
3.4.1 General safety instructions during operation.................................................................................................. 8
3.4.2 Safety instructions– electrical equipment ...................................................................................................... 8
3.4.3 Handling of samples and reagents.................................................................................................................. 9
3.5 Safety instructions–maintenance and repair ................................................................................................ 9
3.6 Behavior during emergencies .......................................................................................................................... 9
4 Design and function........................................................................................................................................................ 10
4.1 Connections and display elements .................................................................................................................. 10
4.2 Device configuration and function................................................................................................................... 12
5 Installation and commissioning..................................................................................................................................... 18
5.1 Installation conditions...................................................................................................................................... 18
5.1.1 Installation location requirements................................................................................................................... 18
5.1.2 Power supply..................................................................................................................................................... 18
5.1.3 Spatial requirements ........................................................................................................................................ 19
5.2 Installing the device.......................................................................................................................................... 19
5.3 Removing and fastening the transport locks .................................................................................................. 20
5.4 Checking and correcting the adjustment......................................................................................................... 21
6 Operation ........................................................................................................................................................................ 23
6.1 Switching the device on and off....................................................................................................................... 23
6.2 Switching the lamps on and off ....................................................................................................................... 24
6.3 Carrying out a first measurement with the SPECORD PLUS .......................................................................... 24
6.4 Accessories........................................................................................................................................................ 27
6.4.1 Cell holders for standard cells.......................................................................................................................... 27
6.4.2 Measuring location for opaque samples ......................................................................................................... 29
6.4.3 Additional accessories ...................................................................................................................................... 30
7 Care and maintenance.................................................................................................................................................... 31
7.1 Cleaning the device........................................................................................................................................... 31
7.2 Replacing lamps and dessicant cartridges....................................................................................................... 31
7.3 Replacing the fuses........................................................................................................................................... 35
8 Transport and storage .................................................................................................................................................... 36
8.1 Moving the device in the laboratory................................................................................................................ 36
8.2 Transport........................................................................................................................................................... 36
8.3 Storage .............................................................................................................................................................. 36

Table of contents SPECORD PLUS
4
9 Disposal ........................................................................................................................................................................... 38
10 Specifications .................................................................................................................................................................. 39
10.1 Physical/optical performance data.................................................................................................................. 39
10.1.1 Optical system................................................................................................................................................... 39
10.1.2 SPECORD 50 PLUS............................................................................................................................................ 39
10.1.3 SPECORD 200 PLUS ......................................................................................................................................... 40
10.1.4 SPECORD 210 PLUS ......................................................................................................................................... 41
10.1.5 SPECORD 250 PLUS ......................................................................................................................................... 42
10.2 General technical data of the SPECORD PLUS................................................................................................ 43
10.3 Standards and directives .................................................................................................................................. 44

SPECORD PLUS About this operating manual
5
1 About this operating manual
Content This user manual provides information on the design and function of the SPECORD
PLUS and the necessary know-how for safe handling of the device and its components.
Furthermore, the user manual includes information on the maintenance and care of the
device as well as indications for potential causes for malfunctions and their correction.
User requirements These instructions are intended for qualified users with knowledge of UV/VIS analysis.
These instructions are limited to describing the functionality of the SPECORD PLUS.
For safe operation of the SPECORD PLUS, knowledge of the "ASpect UV" and "SPECORD
PLUS accessories" operating manuals is also required.
Conventions Instructions for actions are combined into action units and marked with a triangle ( ).
Warnings are indicated by a warning triangle and signal word. The type, source and
consequences of the hazard are stated together with notes on preventing the hazard.
Elements of the control and analysis program are indicated as follows:
¡Program terms are in bold (e.g., the File menu).
¡Buttons are indicated by square brackets (e.g., [Ok]).
¡Menu items are separated by vertical lines (e.g., File | Open)
Symbols and signal words used
in this manual
The user manual uses the following symbols and signal words to indicate hazards or in-
structions. These warnings are always placed before an action.
WARNING
This signal word indicates medium risk hazards which can lead to death or serious in-
juries if not avoided.
CAUTION
This signal word indicates low risk hazards which can lead to minor or moderate injuries
if not avoided.
NOTICE
Provides information on potential material or environmental damage.

Intended use of the SPECORD PLUS SPECORD PLUS
6
2 Intended use of the SPECORD PLUS
The spectrophotometers of the SPECORD PLUS series have been designed for mea-
suring the transmission, extinction, reflectance and energy of liquid, solid and
gaseous samples.
The individual devices have different optical concepts and implement different spec-
tral resolutions:
SPECORD 50 PLUS 1.4nm, split beam with internal reference channel
SPECORD 200 PLUS 1.4nm, double-beam spectrophotometer
SPECORD 210 PLUS 0.2; 0.5; 1; 2 and 4nm, double-beam spectrophotometer
with extended measurement range
SPECORD 250 PLUS 0.2; 0.5; 1; 2 and 4nm, double-beam spectrophotometer
with upstream monochromator
The SPECORD PLUS is compliant with DAB (German pharmacopoeia) regulations as
well as international regulations, such as Ph. Eur., BP, USP or JPXIII.
Together with the ASpect UV software, the SPECORD PLUS is especially suited for use
in routine laboratories with high sample throughput, but also provides solutions for
special applications with its versatile accessories.
The SPECORD PLUS may only be used for the analyses listed in the user manual.
Only this specified use is regarded as the intended use, ensuring the safety of the
user and the device.

SPECORD PLUS Security
7
3 Security
For your own safety and to ensure error-free and safe operation of the device, please
read this chapter carefully before commissioning.
Observe all safety instructions listed in this user manual and all messages and infor-
mation displayed on the monitor by the control and analysis software.
3.1 Safety labeling on the device
Warning and mandatory action labels have been attached to the device and must al-
ways be observed.
Damaged or missing warning and mandatory action labels can cause incorrect ac-
tions leading to personal injury or material damage. The labels must not be removed.
Damaged warning and mandatory action labels must be replaced immediately!
The following warning and mandatory action labels have been attached to the de-
vice:
Warning symbol Meaning Comment
Warning against hot
surface
The lamp covers become very hot
when the lamps are switched on.
Switch off the lamps and allow them
to cool down before replacing the
lamps or opening the lamp covers.
Warning against UV ra-
diation
The lamp radiation can cause eye
damage. Do not look into the beam of
the UV lamp directly or indirectly via a
mirror.
Mandatory signs/
information sym-
bols
Meaning Comment
Disconnect the power
supply before opening
the device cover.
On the power switch/power input: Be-
fore opening the device cover, switch
off the device and disconnect the
mains plug from the mains socket.
Observe the operating
manual
Before starting work, read the operat-
ing manual.
For People's Republic of
China only
The device contains controlled sub-
stances. Analytik Jena GmbH warrants
that these substances will not be re-
leased from the device within the next
25years provided the device is em-
ployed as intended.

Security SPECORD PLUS
8
3.2 Requirements for the operating personnel
The device must only be operated by qualified specialist personnel instructed in the
use of the device. This instruction also include teaching the contents of this user
manual and of the user manuals of the connected system components. We recom-
mend training by qualified employees of Analytik Jena or its representatives.
In addition to the safety instructions in this user manual, the general applicable
safety and accident prevention regulations of the respective country the device is op-
erated in must be observed and adhered to. The operator must ensure the latest ver-
sion of these regulations.
The user manual must be accessible to the operating and service personnel.
3.3 Safety instruction–explosion protection
The device may not be operated in an explosive environment.
3.4 Safety instructions: during operation
3.4.1 General safety instructions during operation
The operator must make sure that the device and its safety equipment is in sound
condition each time before starting up the device. This applies especially after any
modification or adaptation of the device or any repair.
Observe the following:
¡The device may only be operated if all protective equipment (e.g., covers for elec-
tronic components) is in place, properly installed and fully operational.
¡The sound condition of the protection and safety equipment must be checked
regularly. Any defects must be corrected as soon as they occur.
¡Protective and safety equipment must never be removed, modified or switched
off during operation.
¡Ensure that no liquid enters the interior of the device, for example at cable con-
nections. There is a danger of electric shock.
¡Caution when handling glass components. Risk of broken glass and therefore risk
of injury!
¡Never look into the lamp directly or indirectly, e.g., via a mirror. For this reason,
switch off the device when replacing the lamp. A risk of eye damage due to UV
radiation is present.
3.4.2 Safety instructions– electrical equipment
Lethal voltages may occur in the device! Contact with live components may cause
death, serious injury or painful electrical shock.
¡The power plug must be connected to a proper power outlet to ensure that the
device meets protection class I (ground connector). The device may only be con-
nected to power sources whose nominal voltage is the same as that on the rating
plate of the equipment. Do not replace the removable power cable of the device
with a power cable that does not meet the specifications (with no protective
ground conductor). Extensions of the supply cable are not permitted!

SPECORD PLUS Security
9
¡The device, the accessories and the PC may only be connected to the mains net-
work when the device is switched off.
¡Electrical connection cables between the device and the system components may
only be connected or disconnected when the device is switched off.
¡Before opening the device, the device must be switched off via the main switch
and the power plug must be disconnected from the power outlet!
¡Work on the electronics may only be carried out by the customer service of Ana-
lytik Jena and specially authorized technicians.
3.4.3 Handling of samples and reagents
¡The operator is responsible for the selection of substances used in the process, as
well as for their safe handling. This is particularly important for radioactive, in-
fectious, poisonous, corrosive, combustible, explosive and otherwise dangerous
substances.
¡The operator is responsible for carrying out suitable decontamination should the
device be contaminated with dangerous substances on the outside or in the sam-
ple chamber.
¡Splashes, drops or larger reagent spills must be removed and cleaned using an
absorbent material such as laboratory wipes or paper towels.
3.5 Safety instructions–maintenance and repair
¡Maintenance of the SPECORD PLUS is always carried out by the customer service
department of Analytik Jena or its authorized and trained personnel.
¡The user may only perform the maintenance tasks described in this operating
manual (→ "Care and maintenance" 31).
¡The exterior of the SPECORD PLUS may only be cleaned with a damp, not drip-
ping, cloth after the device has been switched off.
¡Always only carry out any maintenance and repair work on the device when it is
switched off (unless otherwise indicated).
¡A risk of burns from hot lamps and surfaces is present. Only carry out mainte-
nance tasks and the replacement of system components (e.g., lamps) after a suf-
ficiently long cooling phase.
¡If necessary, remove the protective cap from the VIS lamp after insertion. A risk
of fire is present!
¡Use only original spare parts, wear parts and consumables. They have been
tested and ensure safe operation. Glass part are wear parts and are not subject
to the warranty.
3.6 Behavior during emergencies
If there is no immediate risk of injury and if this is possible, immediately switch the
device switch of the SPECORD PLUS and the connected system components (PC, ac-
cessories) to the "0" position during dangerous situations or in case of accidents and
disconnect the mains plugs from the mains outlets!
Note: For the PC, there is a risk of data loss and damage to the operating system!

Design and function SPECORD PLUS
10
4 Design and function
4.1 Connections and display elements
The connections and display elements of the SPECORD PLUS are attached to the front
face and the right side wall of the device for easy access. The folding cover of the sample
chamber sits on the sample chamber walls and seals the sample chamber light-proof.
On the bottom side, there are two ducts sealed with plugs for the waste hose of the sip-
per system and the hoses of the temperature-controlled accessories.
Figure1 Connections on the device front
1, 3 Ducts for the waste hose of the sipper system and the hoses of temperature-controlled
cell holders and changers
2 Sample chamber cover
4 Status lights for mains voltage, UV and VIS lamps
The status lamps (LEDs) indicating mains voltage and lamp operation are located on the
right front side of the SPECORD PLUS. The status lamps are lit when the SPECORD PLUS
is switched on or the lamps are on:
¡Green: Mains voltage LED on
¡Yellow: UV lamp LED on
¡Red: VIS lamp LED on

SPECORD PLUS Design and function
11
Figure2 Connections and display elements on the right side of the device
1 Status lamps 2 Mains switch
3 Connection for Peltier-controlled acces-
sories (ACC1)
4 Connection for a second Peltier-con-
trolled accessory and the autosampler
(ACC2)
5 USB-B PC connection 6 RS232 PC connection
7 Socket for mains connection and fuse
holder
Figure3 Lamp housing and type plate on the rear of the device
1 Type plate
2 Service flap for lamp and desiccant cartridge replacement
1
2
3
4
5
6
7
Figure4 Information on the type plate
1 Device type 2 Electrical connection data
3 Fuse type 4 Serial number
5 Do not dispose of device as household
waste
6 CE marking
7 Manufacturer address

Design and function SPECORD PLUS
12
4.2 Device configuration and function
SPECORD PLUS modules The SPECORD PLUS consists of the following modules:
Module Function
Radiation sources The UV lamp (deuterium lamp) and the VIS lamp (halogen lamp)
generate the light that irradiates the sample after monochroma-
tization.
Upstream monochroma-
tor
(SPECORD 250 PLUS
only)
The upstream monochromator is a holographic grid moving syn-
chronously with the monochromator grid. By first dispersing the
light, the stray light is reduced.
Spectrometer system The spectrometer system includes the monochromator which
disperses the light of the radiation sources. After this, the light
emitted from the monochromator is split into a measuring and a
comparison channel. The optics generate the beam cross-section
in the sample chamber. The spectrometer system is protected
against extraneous light and dust by its cover.
SPECORD 200/210/250
PLUS
sample chamber
The samples are measured in the sample chamber. The sample
chamber has two sample chamber rods to hold various accessory
units. The two adapter plates for the cell holders are attached to
the front sample chamber wall.
SPECORD 50 PLUS
sample chamber
Only the measuring beam path is directed through the sample
chamber. The accessories are mounted on the sample chamber
rods. The adapter plate for the cell holder is attached to the front
sample chamber wall.
Receiver circuit board Two photodiodes serve as radiation receivers. The photodiodes
are Peltier-controlled on SPECORD210/250PLUS devices.
Power supply modules The power supply modules provide the electrical voltage for the
electronics and the fans for the thermoelectric temperature con-
trol of the receivers in the SPECORD PLUS.
Device controller PC The device controller coordinates the modules involved in the
measuring process.
Spectrometer system

SPECORD PLUS Design and function
13
1
2
3
4
5
6
7
8
910
11
12
13
14
15
Figure5 SPECORD 200/210/250 PLUS general design
1 VIS lamp 2 Deuterium lamp
3 Upstream monochromator grid or toric
lighting mirror
4 Filter wheel
5 Entrance slit 6 Concave grid
7 Exit slit 8 Beam splitter
9 Toric mirror 10 Toric mirror
11 Plane mirror 12 Sample chamber
13 Cells 14 Measuring locations for opaque samples
15 Receiver

Design and function SPECORD PLUS
14
1
2
3
4
5
6
7
8
910
11
12 13
14
15
Figure6 SPECORD 50 PLUS general design
1 VIS lamp 2 UV lamp
3 Toric mirror 4 Filter wheel
5 Entrance slit (hidden) 6 Concave grid
7 Exit slit 8 Spherical mirror
9 Plane mirror 10 Quartz plate
11 Comparison channel receiver 12 Sample chamber
13 Cell 14 Measuring location for opaque samples
15 Measuring channel receiver
The spectrometer system with the filter wheel, concave grid and slit group elements
shown above functions as a monochromator.
The following filters are mounted on the filter wheel:
Filter Function
Colored glass filters Suppression of the undesired radiation in the monochromator
Holmium filter Standard for the automatic recalibration of the wavelengths
2 empty slots Allows undispersed light to pass through
Locking cone Dark signal measurement
The inlet and outlet slits of the spectrometer system are mounted on the slit carrier. The
following spectral slit widths are implemented in this manner on the various device
types:
Device Slit widths
SPECORD 50 PLUS 1.4nm
SPECORD 200 PLUS 1.4nm
SPECORD 210/250
PLUS
0.2; 0.5; 1; 2; 4nm.
The imaging grid disperses the incoming light and fans out the spectrum.
Computer-controlled step motors drive the filter wheel, slit carrier and linear actuator
for the grid movement. The low number of moving parts in the spectrometer system
leads to high reliability of the optical parameters of the SPECORD PLUS.

SPECORD PLUS Design and function
15
In the photometer chamber of the SPECORD 200/210/250 PLUS, the fixed 50/50
beam divider splits the beam arriving from the monochromator into a measuring and
reference beam. The SPECORD 50 PLUS uses the portion of the radiation reflected on a
quartz plate as an internal reference to compensate for intensity fluctuations. The imag-
ing mirrors generate the necessary beam cross-sections in the sample chamber.
Sample chamber The large sample chamber of the SPECORD PLUS has a variable design. The measuring
and reference beam enter the sample chamber of the SPECORD 200/210/250 PLUS
from the rear. Two windows protect the photometer chamber against dust and contami-
nation from reagents.
In the SPECORD 50, the high energy measuring beam goes though the middle of the
sample chamber. The reference beam for compensating energy fluctuations is directed
internally to the receiver diode.
The holders for standard cells are slid directly into the adapter plates on the front sam-
ple chamber wall and are located close to the receiver. Cells with opaque samples of
10mm layer thickness can be placed in special cuvette ducts directly in front of the re-
ceiver.
As an optical bench, the support rod system provides space for large accessories, e.g.,
the 8-cell changer or the integrating sphere. Additionally, your own experimental con-
structs can be placed in the sample chamber in an optically reproducible manner and ex-
changed.
In the right sample chamber wall are the connection sockets for the electrical accessories
or the identification connectors for the accessories.
Two large ducts closed with plugs are located at the bottom of the front sample cham-
ber wall. The waste hose of the sipper system or the hoses for temperature-controlled
accessories can be routed through these ducts. Other ducts of varying sizes are located in
both the left and right sample chamber walls. These are closed via white plugs in a rub-
ber plug. The connection hoses for the sipper system, temperature-controlled acces-
sories and optical cables for external sample probes can be routed through these ducts.
After removing the attachment screws, the side components of the sample chamber can
be removed and exchanged to convert the spectrometer to a flow measurement system
or to use air-cooled Peltier accessories.
The beam paths in the SPECORD PLUS and the connections for the electrical accessories
are marked:
¡"M" – Measuring beam path or connection for accessories in the measuring beam
path
¡"R" – Reference beam path or connection for accessories in the reference beam path

Design and function SPECORD PLUS
16
Figure7 SPECORD 200/210/250 PLUS sample chamber
1 Measuring beam path 2 Reference beam path
3 Connections for electrical accessories 4, 11 Removable side components
5, 10 Attachment screws for side components 6, 9 Plates for holding the cell holders
7, 8 Cell ducts for dispersing samples
Figure8 Right sample chamber wall
1 Connections for electrical accessories 2 Removable side component
3 Ducts for accessory hoses and cables 4 Side component attachment screw

SPECORD PLUS Design and function
17
Figure9 SPECORD 200/210/250 PLUS sample chamber dimensions
Figure10SPECORD 50 PLUS sample chamber
1 Measuring beam path 2 Connections for electrical accessories
3, 8 Removable side components 4, 7 Side component attachment screws
5 Plate for holding the cell holder 6 Cell duct to hold dispersing samples
Figure11SPECORD 50 PLUS sample chamber dimensions

Installation and commissioning SPECORD PLUS
18
5 Installation and commissioning
5.1 Installation conditions
5.1.1 Installation location requirements
Ambient conditions The climate conditions for the installation location are listed in the technical specifica-
tions (→ "General technical data of the SPECORD PLUS" 43). If required, ensure that
the room is temperature-controlled.
Laboratory conditions The device is only approved for indoor use. The installation location should have the
characteristics of a chemical laboratory. It must meet the following conditions:
¡Environment with low dust levels
¡No vibrations
Installation location require-
ments
The requirements for the installation location of the device are as follows:
¡No caustic vapor in the immediate vicinity of the device and its system components.
These can corrode the device connections and modules.
¡No drafts; do not install the device close to windows or doors
¡Away from electromagnetic sources of interference
¡No direct sunlight and away from radiant heaters
¡Do not obstruct the air vents of the device with other equipment
5.1.2 Power supply
WARNING
Risk of electric shock
The device may only be connected to a properly earthed power outlet in accordance with
the voltage specifications on the type plate.
The SPECORD PLUS operates on single-phase alternating current.
Electrical connection require-
ments Operating voltage 85 to 264 V/AC
Line frequency 50 to 60 Hz
Power consumption 200 VA
Device fuses T 3.15AH/250V, type 19181

SPECORD PLUS Installation and commissioning
19
5.1.3 Spatial requirements
Figure12SPECORD PLUS dimensions
The device is designed as a tabletop device and weighs approximately 22kg. A stable
table with a load capacity of 50kg is recommended as the installation location.
The device requires a minimum installation surface area of 680 x 720mm due to its di-
mensions and the required approx. 10cm clearance on the side of the electrical connec-
tions. Also, provide sufficient space for possible accessories and the control computer
The ventilation slits on the top and bottom sides of the device must not be covered.
Device dimensions Device Width x length x height
SPECORD PLUS 590 x 690 x 290mm
APG autosampler (optional) 500 x 540 x 470mm
Temperature control device for Peltier-con-
trolled accessories (optional)
225 x 200 x 130mm
Heat exchanger for Peltier-controlled acces-
sories (optional)
225 x 200 x 175mm
The temperature control device and the heat exchanger can be stacked upon each other
to save space.
5.2 Installing the device
WARNING
Risk of electric shock
Check that the mains connection conditions match those indicated on the type plate on
the rear of the device.
}Remove the SPECORD PLUS and accessories from the transport packaging. Remove
the protective sleeve from the device.
Caution! Transport the SPECORD PLUS in upright position only and do not overturn
it when unpacking. Observe the markings on the transport packaging.
}Place the device and the PC on a suitable surface (laboratory desk).
}Allow the device to acclimatize until it has reached the ambient temperature.
}Remove the transport lock (→ "Removing and fastening the transport locks" 20).
}Connect one mains cable each to the SPECORD PLUS and the PC. Connect mains con-
nectors to sockets connected to the same power supply phase (if possible via socket
strips).
Do not connect the USB cable yet.

Installation and commissioning SPECORD PLUS
20
}Switch on the PC and install the ASpect UV program (see "ASpect UV software for
SPECORD PLUS" operating manual).
}Connect the USB port of the SPECORD PLUS and the PC with the supplied USB cable.
Switch on the SPECORD PLUS and start ASpect UV.
When the SPECORD PLUS is connected for the first time, a device detection proce-
dure is performed and the AJ driver is assigned after the device is switched on.
}After initialization of the SPECORD PLUS and activation of the lamps, wait for a run-
in time of 2hours and then adjust the device with the aid of the software (→ "Check-
ing and correcting the adjustment" 21).
üThe SPECORD PLUS is now ready for operation.
5.3 Removing and fastening the transport locks
NOTICE
Before commissioning the SPECORD PLUS, remove or disable all transport locks!
The SPECORD PLUS has been fitted with transport locking screws on the monochroma-
tor and with foam blocks in the sample chamber for transport to prevent damage to the
device and its optics. The locking screw of the monochromator is located on the right
rear device side on the base plate. The SPECORD 250 PLUS has a second locking screw
to protect the upstream monochromator on the left rear device side. The Allen key for
removing the locking screw is inserted in one of the foam blocks in the sample chamber.
Removing the transport locks }Remove the white foam blocks between the sample chamber rods and take the Allen
key out of the block.
Figure13Foam blocks protecting the support rods
}Turn the transport locking screw on the left rear side counterclockwise (to "free" on
the sticker) with the Allen key until it stops.
}SPECORD 250 PLUS only
Turn the second transport locking screw protecting the upstream monochromator on
the right rear side counterclockwise with the Allen key until it stops.