GE MOA 280i User manual

Sievers*Nitric Oxide Analyzer NOA 280i
Operation and
Maintenance Manual
Firmware Version 3.00 and later
6060 Spine Road
Boulder, CO 80301 USA
phone [phone] • 303.444.2009
fax [phone] DLM 14291 Rev A
www.geinstruments.com Printed in USA ©2006

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IDENTIFICATION RECORDS
Record the following numbers as they are listed on the identification labels
located on the back panel of the NOA and the front of the vacuum pump.
Analyzer serial no.
Pump serial no.
Warranty Start Date
Date of receipt/Installation
*Trademark of General Electric Company; may be registered in one or more countries.

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PRINTING HISTORY
The information contained in this document may be revised without notice.
Sievers Instruments makes no warranty of any kind with regard to this material.
Sievers Instruments shall not be liable for errors contained herein or for incidental,
or consequential damages in connection with the furnishing, performance or use of
this material.
No part of this document may be photocopied or reproduced without the prior
written consent of Sievers Instruments, Inc.
Initial Printing December 2000
DLM 14290-01 Revision A March 2001
DLM 14291 Revision A May 2006
Printed in USA

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Standard Limited Warranty
GE Analytical Instruments warrants its products (Sievers®, GE Analytical™ and
Leakwise™) against defects in materials and workmanship. GE Analytical
Instruments will, at its option, repair or replace instrument components that prove
to be defective with new or remanufactured components (i.e., equivalent to new).
The warranty set forth is exclusive and no other warranty, whether written or
oral, is expressed or implied.
Warranty Term
The GE Analytical Instruments warranty term is thirteen (13) months ex-works, or
twelve (12) months from installation or start up by GE Analytical Instruments
certified service personnel. In no event shall the standard limited warranty
coverage extend
beyond thirteen (13) months from original shipment date.
Warranty Service
Warranty Service is provided to customers through telephone support (1-800-255-
6964), Monday - Friday, from 8:00 a.m. to 5:00 p.m. (Mountain Time), excluding
all company and legal holidays. Telephone support is provided for troubleshooting
and determination of parts to be shipped from GE Analytical Instruments to the
customer in order to return the product to operation. If telephone support is not
effective, the product may be returned to GE Analytical Instruments for repair or
replacement. In some instances, suitable instruments may be available for short
duration loan or lease.
GE Analytical Instruments warrants that any labor services provided shall conform
to the reasonable standards of technical competency and performance effective at
the time of delivery. All service interventions are to be reviewed and authorized
as correct and complete at the completion of the service by a customer
representative, or designate. GE Analytical Instruments warrants these services for
30 days after the authorization and will correct any qualifying deficiency in labor
provided that the labor service deficiency is exactly related to the originating

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event. No other remedy, other than the provision of labor services, may be
applicable.
Repair components (parts and materials), but not consumables, provided in the
course of a repair, or purchased individually, are warranted for 90 days ex-works
for materials and workmanship. In no event will the incorporation of a warranted
repair component into an instrument extend the whole instrument’s warranty
beyond its original term.
Consumables (e.g., dilution standards, verification solutions, and UV lamps, etc.)
are warranted to the extent of their stated shelf life, provided these items are
maintained within the stated environmental limitations. Warranty claims for
consumables and verification standards are limited to the replacement of the
defective items, prorated from the time of claim to the expiration of shelf life.
Shipping
A Repair Authorization Number (RA) must be obtained from the Technical Support
Group before any product can be returned to the factory. GE Analytical
Instruments will pay freight charges, exclusive of any taxes and duties, for
replacement or repaired products shipped to the customer site. Customers shall
pay freight charges, including all taxes and duties, for all products returning to GE
Analytical Instruments. Any product returned to the factory without an RA number
will be returned to the customer.

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TABLE OF CONTENTS
1. INTRODUCTION...................................................................................................................................... 1-1
SAMPLE INLET SYSTEMS ..................................................................................................................................... 1-5
SAMPLE FLOW CONTROL DEVICE........................................................................................................................ 1-6
OZONE FLOW CONTROL MODULE....................................................................................................................... 1-6
OZONE GENERATOR.......................................................................................................................................... 1-6
CHEMILUMINESCENT REACTION CHAMBER AND OPTICAL FILTER ............................................................................ 1-6
PHOTOMULTIPLIER TUBE AND COOLED HOUSING ................................................................................................. 1-7
VACUUM PUMP AND OZONE DESTRUCTION TRAP................................................................................................. 1-7
ELECTRONICS.................................................................................................................................................... 1-7
ANALOG, PRINTER AND RS-232 OUTPUTS ......................................................................................................... 1-8
EXHALATION PRESSURE TRANSDUCER ................................................................................................................. 1-9
THERMAL MASS FLOW METER ............................................................................................................................ 1-9
2. SPECIFICATIONS..................................................................................................................................... 2-1
3. MENUS AND CONTROL OVERVIEW ..................................................................................................... 3-1
MAIN MENU...................................................................................................................................................... 3-1
STATUS SCREEN................................................................................................................................................ 3-1
ANALYSIS ......................................................................................................................................................... 3-2
MEASUREMENT MENU ........................................................................................................................................ 3-3
MAIN MENU OPTIONS........................................................................................................................................ 3-6
Control..................................................................................................................................................... 3-6
Calibration.............................................................................................................................................. 3-7
Messages.................................................................................................................................................. 3-7
Maintenance............................................................................................................................................ 3-8
TIME-OUT FUNCTION ........................................................................................................................................ 3-8
4. INSTALLATION........................................................................................................................................ 4-1
LOCATION ........................................................................................................................................................ 4-1
POWER REQUIREMENTS ..................................................................................................................................... 4-1
ENVIRONMENTAL CONSIDERATIONS..................................................................................................................... 4-2
TOOLS AND ADDITIONAL SUPPLIES...................................................................................................................... 4-2
Tools......................................................................................................................................................... 4-2
Gases........................................................................................................................................................ 4-2
Data Collection....................................................................................................................................... 4-2
VACUUM PUMP SETUP....................................................................................................................................... 4-4

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Step 1 – Add Oil to the pump...............................................................................................................4-4
Step 2 - Install Pump Inlet Fitting ......................................................................................................4-4
Step 3- Install the Chemical Trap Mounting Bracket.......................................................................4-5
Step 4 - Install the Pump Outlet Fitting............................................................................................ 4-6
Step 5 - Install the Chemical Trap and Vacuum Hoses....................................................................4-6
Step 6 - Connect Power Cord to Vacuum Pump and Turn On Pump Power Switch ....................4-9
CONNECTIONS TO NOA.....................................................................................................................................4-9
Vacuum Pump Power Cord and Vacuum Hose................................................................................... 4-9
Vacuum Test............................................................................................................................................4-9
Gas for Ozone Generator....................................................................................................................4-11
Frit Restrictor....................................................................................................................................... 4-12
Computer, Printer and Analog Signal Connections........................................................................ 4-12
Setting the Clock..................................................................................................................................4-13
CONFIGURATION MENU OPTIONS......................................................................................................................4-14
Com Port................................................................................................................................................4-14
Pressure Units.......................................................................................................................................4-15
SETTING THE CONSUMABLES INSTALLATION DATA..............................................................................................4-16
START-UP.......................................................................................................................................................4-16
5. INSTALLATION AND SETUP: GAS-PHASE MEASUREMENTS............................................................5-1
INSTALLATION OF GAS SAMPLING PACKAGE.........................................................................................................5-1
INSTALLATION OF THERMAL MASS FLOWMETER....................................................................................................5-2
NOA SETUP FOR GAS-PHASE MEASUREMENTS..................................................................................................... 5-4
Exhalation Mode.....................................................................................................................................5-4
Nitric Oxide Mode..................................................................................................................................5-7
6. CALIBRATION..........................................................................................................................................6-1
ZERO GAS CALIBRATION ....................................................................................................................................6-1
Calibration with Zero Air Filter..........................................................................................................6-2
Calibrating with Zero Air Cylinder.....................................................................................................6-2
Zero Gas Calibration Warnings............................................................................................................ 6-4
NO CALIBRATION GAS.......................................................................................................................................6-4
Calibration Gas Warnings.....................................................................................................................6-6
Calculation of Gas Concentration.......................................................................................................6-8
INDEPENDENT CALIBRATION OF PPB AND PPM RANGES ..........................................................................................6-8
ACCURACY OF PPB LEVEL MEASUREMENTS USING PPM LEVEL CALIBRATION ............................................................. 6-9
FLOW/RESPONSE CHARACTERISTICS OF NOA 280I...........................................................................................6-10
CALIBRATION AT LOWER FLOW RATES ..............................................................................................................6-11

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7. ON-LINE EXHALED NITRIC OXIDE ....................................................................................................... 7-1
ASSEMBLY OF THE ACCURATE NO BREATH KIT.................................................................................................... 7-2
CONNECTION OF THERMAL MASS FLOWMETER..................................................................................................... 7-3
CONNECTION OF GAS SAMPLING AND PRESSURE TUBING ...................................................................................... 7-3
INSPIRATORY GAS CONNECTIONS........................................................................................................................ 7-4
Inspiratory Gas Filter............................................................................................................................ 7-4
NOA SETUP..................................................................................................................................................... 7-4
PERFORMING THE MANEUVER............................................................................................................................. 7-5
SELECTION OF NO PLATEAU.............................................................................................................................. 7-6
FLOW/PRESSURE CHARACTERISTICS OF ACCURATE NO RESTRICTORS ................................................................... 7-8
MODELS OF NITRIC OXIDE PRODUCTION IN THE AIRWAYS ..................................................................................... 7-8
CLEANING THE ACCURATE NO BREATH KIT AND FLOWMETER............................................................................... 7-9
Disassemble the Valve........................................................................................................................... 7-9
Prewash the Components................................................................................................................... 7-10
Sterilization.......................................................................................................................................... 7-10
Rinsing.................................................................................................................................................... 7-10
Drying..................................................................................................................................................... 7-10
CHECKING THE INSPIRATORY GAS FILTER.......................................................................................................... 7-11
8. OFF-LINE EXHALED NITRIC OXIDE (BAG SAMPLING) ...................................................................... 8-1
ASSEMBLY OF VITAL CAPACITY BAG COLLECTION KIT........................................................................................... 8-1
ASSEMBLY OF DEADSPACE DISCARD BAG COLLECTION KIT .................................................................................... 8-2
CLEANING THE BAGS......................................................................................................................................... 8-5
COLLECTING THE SAMPLES – VITAL CAPACITY BAG KIT........................................................................................ 8-6
Connecting the bag to the filler......................................................................................................... 8-7
Instructing the Subject and Collecting the Samples ....................................................................... 8-7
Disconnecting the bag from the filler and sealing the bag........................................................... 8-8
COLLECTING THE SAMPLES – DEADSPACE DISCARD BAG KIT.................................................................................. 8-9
Connecting the bag to the filler......................................................................................................... 8-9
Instructing the Subject and Collecting the Samples ..................................................................... 8-10
Disconnecting the bag from the filler and sealing the bag......................................................... 8-11
ANALYZING THE SAMPLES ................................................................................................................................8-11
Analysis Setup....................................................................................................................................... 8-11
NOA SETUP................................................................................................................................................... 8-11
CLEANING THE BAG KITS ................................................................................................................................. 8-13
Vital Capacity Bag Kit......................................................................................................................... 8-13
Deadspace Discard Bag Kit ................................................................................................................. 8-14

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FLOW/PRESSURE CHARACTERISTICS OF BAG KITS..............................................................................................8-14
STABILITY OF NO IN MYLAR BAGS....................................................................................................................8-15
OFF-LINE VERSUS ON-LINE EXHALED NO MEASUREMENTS.................................................................................. 8-17
TESTING BAGS FOR PINHOLE LEAKS..................................................................................................................8-17
9. BREATH-BY-BREATH AND CHAMBER SAMPLING FOR EXHALED NITRIC OXIDE..........................9-1
BREATH-BY-BREATH MEASUREMENTS..................................................................................................................9-1
Spontaneously Breathing Subjects......................................................................................................9-1
Ventilated Subjects...............................................................................................................................9-2
NOA SETUP.....................................................................................................................................................9-3
NO/Pressure Offset................................................................................................................................9-3
Humidified Circuits................................................................................................................................9-3
CHAMBER SAMPLING..........................................................................................................................................9-4
NOA SETUP.....................................................................................................................................................9-6
10. NASAL NITRIC OXIDE......................................................................................................................10-1
RECOMMENDED SETUP.....................................................................................................................................10-1
PERFORMING THE MANEUVER........................................................................................................................... 10-2
NOA SETUP...................................................................................................................................................10-3
11. INSTALLATION AND SETUP: LIQUID MEASUREMENTS..............................................................11-1
SUPPLIES........................................................................................................................................................11-1
Gases......................................................................................................................................................11-1
Reagents................................................................................................................................................ 11-1
Lab Equipment......................................................................................................................................11-1
SETUP OF PURGE VESSEL................................................................................................................................. 11-2
Connections of tubing to glassware..................................................................................................11-3
Procedure for Tightening Swagelok Fittings.................................................................................................11-4
DILUTION OF ANTI-FOAMING AGENT................................................................................................................. 11-7
NOA SETUP FOR LIQUID MEASUREMENTS..........................................................................................................11-8
DEPROTEINIZATION PROCEDURES....................................................................................................................11-10
Cold ethanol precipitation...............................................................................................................11-11
Zinc Sulfate/Sodium Hydroxide precipitation..............................................................................11-11
12. MEASUREMENT OF NITRIC OXIDE AND NITRITE IN LIQUID SAMPLES....................................12-1
APPARATUS FOR NITRITE REDUCTION ...............................................................................................................12-1
PREPARATION OF THE NITRITE REDUCING AGENT ..............................................................................................12-2
ADJUSTMENT OF PURGE GAS FLOW RATE .........................................................................................................12-3