GE EPS 301 User manual

Electrophoresis Power Supply
EPS 301
GE Healthcare
User Manual
Manuel d’Utilization
Bedienungsanleitung
Manual del usuario
Manuale dell’operatore

Fig 1. The front panel of the EPS 301.
Fig 1. Le panneau avant de l’EPS 301.
Abb 1. Die Fronttafel des EPS 301.
Fig 1. Panel frontal de la EPS 301.
Fig 1. Pannello anteriore dell’EPS 301.
Fig 2. The rear panel of the EPS 301.
Fig 2. Le panneau arrière de l’EPS 301.
Abb 2. Die Rückwand des EPS 301.
Fig 2. Panel posterior de la EPS 301.
Fig 2. Pannello posteriore dell’EPS 301.
100-120 220-240
Voltage
Frequency
Power
100-120/220-240 V AC
50/60 Hz
120W 1.5 A MAX
Certified in accordance with IEC 1010-1
by SEMKO AB, Sweden
MAINS
Disconnect before servicing
HV on
Electrophoresis Power Supply - EPS 301
view
STOP
run
set
enter

Important user information
All users must read this entire manual to fully understand the
safe use of EPS 301.
WARNING!
The WARNING! sign highlights instructions that must
be followed to avoid personal injury. It is important
not to proceed until all stated conditions are met and
clearly understood.
CAUTION!
The CAUTION! sign highlights instructions that must be followed
to avoid damage to the product or other equipment. It is
important not to proceed until all stated conditions are met and
clearly understood.
Note
The Note sign is used to indicate information important for
trouble-free and optimal use of the product.
CE Certifying
This product meets the requirements of applicable CE-directives.
A copy of the corresponding Declaration of Conformity is
available on request.
The CE symbol and corresponding declaration of conformity, is
valid for the instrument when it is:
– used as a stand-alone unit, or
– connected to other CE-marked GE Healthcare instruments, or
– connected to other products recommended or described in
this manual, and
– used in the same state as it was delivered from GE Healthcare
except for alterations described in this manual.
Recycling
This symbol indicates that the waste of electrical
and electronic equipment must not be disposed
as unsorted municipal waste and must be
collected separately. Please contact an authorized
representative of the manufacturer for information
concerning the decommissioning of your equipment.
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Safety Standards
This product meets the requirement of the Low Voltage Directive
(LVD) 73/23/EEC through the following harmonized standards:
• EN 61010-1
• IEC 61010-1
• CAN/CSA-C22.2 No. 61010-1
• UL61010-1
EMC Standards
This device meets the requirements of the EMC Directive 89/336/
EEC through the following harmonized standards:
• EN 61326 (emission and immunity)
• EN 55011, GR 1, Class A (emission)
• This device complies with part 15 of the FCC rules (emission).
Operation is subject to the following two conditions:
1 This device may not cause harmful interference.
2 This device must accept any interference received,
including interference that may cause undesired
operation.

4Electrophoresis Power Supply EPS 301 User Manual 18-1130-18 Edition AC
ENGLISHFRANCEDEUTCHESPAÑOLITALIANO
Contents
ENGLISHFRANCEDEUTCHESPAÑOLITALIANO
Contents
1 Introduction
2 Safety information
2.1 Safety precautions........................................................................................................................ 6
2.2 Built-in safety features................................................................................................................ 6
3 Unpacking and installation
3.1 Unpacking ......................................................................................................................................... 7
3.2 Mains connection........................................................................................................................... 7
3.3 Connection of the electrophoresis unit(s)........................................................................... 7
4 Technical description
4.1 Materials............................................................................................................................................. 9
4.2 Front panel........................................................................................................................................ 9
4.3 Display................................................................................................................................................. 9
4.4 Keyboards ......................................................................................................................................... 9
4.5 Output sockets ..............................................................................................................................10
4.6 Rear panel .......................................................................................................................................10
5 Operation
5.1 Connecting the electrophoresis unit(s) ..............................................................................11
5.2 Programming.................................................................................................................................11
5.3 Running.............................................................................................................................................11
5.4 Short instructions.........................................................................................................................12
6 Maintenance
6.1 Recycling..........................................................................................................................................14
7 Trouble shooting
7.1 Mains power failure ....................................................................................................................15
7.2 Serious error...................................................................................................................................15
7.3 Recycling..........................................................................................................................................15
8 Technical specifications
9 Ordering information

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Introduction 1
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Electrophoresis Power Supply EPS 301 User Manual 18-1130-18 Edition AC
The GE Healthcare Electrophoresis Power Supply EPS 301 is a high quality, high precision
and safe power supply for electrophoresis applications that require a maximum of
300 volts, 400 mA and 80 W.
EPS 301 is primarily designed for the following techniques:
1. Submarine DNA electrophoresis
2. Electroblotting
3. Immuno electrophoresis
4. Mini polyacrylamide gel electrophoresis
Electrophoresis separations can be controlled by voltage or current. The EPS 301
automatically switches over the controlling parameter according to programmed limits
and conductivity variations in the system.
Two electrophoresis units can be connected to the EPS 301 and run with the same
programmed method at one time.
1 Introduction

6Electrophoresis Power Supply EPS 301 User Manual 18-1130-18 Edition AC
2 Safety information
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2.1 Safety precautions
WARNING! Because this instrument can develop sufficient voltage and current to
produce a lethal shock, extreme caution should be exercised in its operation.
The power supply should only be used by properly trained operators. Read this entire
manual before using this power supply and use only according to the manufacturer’s
instructions.
1. This instrument is designed for indoor use only.
2. The instrument must always be used with the earth lead of the power cord correctly
grounded to earth at the mains outlet.
3. To permit sufficient cooling, ensure that the vents in the rear and sides of the
instrument are not covered.
4. Do not operate the instrument in extreme humidity (above 95%). Avoid
condensation by letting the unit equilibrate to ambient temperature when taking
the power supply from a colder to a warmer environment.
5. Keep the instrument as dry and clean as possible. Wipe regularly with a soft damp
cloth. Let the power supply dry completely before use. If wetted, unplug the power
supply until the instrument is dry.
6. Use only undamaged electrical wire and equipment specified for the voltages you
will use. High voltage electrical wires should be in accordance with IEC 1010-2-
031:1993. All equipment connected to high voltage should be in accordance with
IEC 1010-1:1993.
2.2 Built-in safety features
The EPS 301 has been tested and complies with the IEC 61010-1 (EN 61010-1) electrical
safety standard.
2 Safety information

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Unpacking and installation 3
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Electrophoresis Power Supply EPS 301 User Manual 18-1130-18 Edition AC
3.1 Unpacking
Check the contents against the packing list supplied. Inspect for any damage that may
have occurred during transit. Report any damage immediately to your local
GE Healthcare representative and to the transport company concerned.
3.2 Mains connection
Select the appropriate voltage range, 100–120 or 220–240 V. See fig 2 inside front cover.
WARNING! If the power supply is connected to 220–240 V with the range set to
100–120 V, the instrument can be severely damaged.
WARNING! Only use mains cables delivered or approved by GE Healthcare.
WARNING! Do not block the rear panel of the system. The mains power switch must
always be easy to access.
Select the appropriate mains cable and connect one end to the mains socket on the
EPS 301 power supply, see fig 2 inside front cover, and the other end to an AC grounded
outlet.
Switch on the power. Each time the instrument is turned on a self diagnostic test is done.
If an error is detected during the test a message will appear on the display and an alarm
will sound.
3.3 Connection of the electrophoresis unit(s)
Connect the leads from the electrophoresis unit (red to red, and black or blue to blue).
The red lead is the positive and black or blue is the negative.
WARNING! Use only undamaged electrical cables and equipment approved for the
voltage you will use. High voltage electrical cables should be in accordande with
IEC 1010–2–031:1993. All equipment connected to high voltage electrical cables
should be in accordance with IEC 1010-1:1993.
3 Unpacking and installation

8Electrophoresis Power Supply EPS 301 User Manual 18-1130-18 Edition AC
3 Unpacking and installation
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Local regulation for Great Britain
IMPORTANT WARNING
This appliance must be earthed.
The wires in the mains lead are coloured in accordance with the following code:
Green and yellow Earth
Blue Neutral
Brown Live
If the plug provided is unsuitable for your socket outlets, the plug must be cut off
and a suitable plug fitted. The cut-off plug should be disposed of and must not be
inserted into any socket as this can result in electric shock. The plug or adapter or
the distribution panel should be provided with a 13 amp fuse. As the colours of the
wires in the mains lead of this appliance may not correspond with coloured markings
identifying the terminals in your plug, proceed as follows:
The green and yellow wire must be connected to the terminal in the plug which is
marked with the letter E or by the earth symbol, or coloured green, or green and
yellow.
The blue wire must be connected to the terminal which is marked with the letter N or
coloured black.
The brown wire must be connected to the terminal which is marked with the letter L
or coloured red.
Note: After replacing or changing a fuse, the fuse cover in the plug must be replaced
with a fuse cover which corresponds to the colour of the insert in the base
of the plug or the word that is embossed on the base of the plug, and the
appliance must not be used without a fuse cover.
Only 13 Amp fuses approved to B.S. 1362 A.S.T.A. should be used.

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Technical description 4
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Electrophoresis Power Supply EPS 301 User Manual 18-1130-18 Edition AC
4.1 Materials
The main components in the EPS 301 power supply are made of the following materials.
Instrument box Aluminium
Display holder Polypropene (PP). UL94V0
Keyboard Polyester
Output sockets Acetalplastic (POM). UL94V0
Rubber feet Polyurethane
4.2 Front panel
On the front panel there is a numerical display illustrated with the symbols, V, mA, and
h.m., a keyboard with six membrane keys, a light emitting diode that lights when voltage
is applied (HV on) and output sockets for two electrophoresis units.
4.3 Display
A four digit numeric display guides you through the programming, shows actual
parameter values during the electrophoresis and final parameter values afterwards. It
also shows error messages.
Figure 1, inside front cover, shows the display in the End position when power is switched
on.
4.4 Keyboards
SET/ENTER
Puts the instrument into its program mode. Pressing SET/ENTER in the program mode
enters a value, if valid, and moves the program to the next parameter.
In the RUN mode, pressing SET/ENTER allows you to make changes in the program
during a run.
Valid values are: voltage 5–300 V, current 10–400 mA, time 0.01–23.59 h, or timer
off - - - -.
UP/DOWN
Changes the parameter value V, mA, and h.m. when the display is flashing. Numerical
values are changed in an accelerating manner when the key is held down. Clicking /
changes the value in pre-set increments. The values will automatically change from
maximum to minimum value or vice versa, except for the time (h.m.) where the
signs - - - - are placed between the minimum and maximum values indicating that the
timer is switched off.
4 Technical description
set
enter
/

10 Electrophoresis Power Supply EPS 301 User Manual 18-1130-18 Edition AC
4 Technical description
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RUN
Starts the run and puts the program into RUN mode. The light (HV on) is switched on.
VIEW
In run mode, VIEW switches between the actual values for voltage, current and elapsed
time. Pressing VIEW for more than three seconds starts automatic switching between the
actual values for voltage, current and elapsed time. This automatic switching is stopped
by pressing VIEW or RUN.
In End mode, VIEW switches between the end parameter values.
STOP
Stops the run and puts the instrument in End mode. The voltage and the light (HV on) are
switched off. By pressing VIEW the end parameter values are displayed.
4.5 Output sockets
There are two sets of output sockets connected in parallel to allow two electrophoresis
units to be connected and run at the same time, see fig. 1 inside front cover.
4.6 Rear panel
The rear panel is shown in fig 2, see inside front cover. On the rear panel there is:
1. A mains switch. Press in I to switch on the power to the power supply. Press 0 to
switch off the power.
2. A socket for the mains cable.
3. A switch for voltage range. The left position corresponds to 100–120 V and the right
to 220–240 V.
4. Vents.
run
view
STOP