Gilson PIPETMAN P Manual

Decontamination Procedures
for
Gilson Pipettes
(defined for Users to Incorporate in SOPs)
in Accordance with ISO8655 Standard
Procedure LT802288/C - © 2003 Gilson SAS All rights reserved October 2003
Standard Operating Procedure for Pipettes

Page 2
Contents
II
II
Inn
nn
ntrtr
trtr
troducoduc
oducoduc
oductiontion
tiontion
tion 3
Decontamination Room 3
Equipment and Clothing 3
Solutions for Decontaminating and Cleaning 4
Precautions 4
PP
PP
Prr
rr
rococ
ococ
oc
eduredur
eduredur
edure fe f
e fe f
e for End-useror End-user
or End-useror End-user
or End-user’’’’’ss
ss
s5
CC
CC
Completomplet
ompletomplet
omplete Pe P
e Pe P
e Prr
rr
rococ
ococ
oc
eduredur
eduredur
edure fe f
e fe f
e for Gilson Distror Gilson Distr
or Gilson Distror Gilson Distr
or Gilson Distributibut
ibutibut
ibutorsors
orsors
ors 6
Checking the Outside of the Package for Radioactivity 6
Unpacking the Pipettes and Checking for Radioactivity 6
Disassembly PDisassembly P
Disassembly PDisassembly P
Disassembly Prr
rr
rococ
ococ
oc
eduredur
eduredur
edureses
eses
es 8
DD
DD
Decec
ecec
econon
onon
ontaminatamina
taminatamina
tamination Mtion M
tion Mtion M
tion Methodsethods
ethodsethods
ethods 10
Manual Method 10
Immersion Method 11
AA
AA
Associassocia
ssociassocia
ssociatt
tt
ted Ded D
ed Ded D
ed Documenocumen
ocumenocumen
ocumentsts
tsts
ts 12
NotNot
NotNot
Noteses
eses
es 13

Page 3
Introduction
This document contains Decontamination Procedures suitable for
Pipetman® P, Pipetman® F, Pipetman® Ultra (Single and Multichannel),
Pipetman® 8X200, Microman® and Distriman®; it describes how to
decontaminate specific component parts of these pipettes.
Only after preliminary checks for radioactivity may the pipette be
unpacked and disassembled for subsequent cleaning and decontami-
nation, using disposable gloves.
DD
DD
Decec
ecec
econon
onon
ontaminatamina
taminatamina
tamination startion star
tion startion star
tion starts with rts with r
ts with rts with r
ts with radioacadioac
adioacadioac
adioactivittivit
tivittivit
tivity checksy checks
y checksy checks
y checks using a
validated Geiger-Müller counter equipped with probes capable of
detecting gamma, beta and X-rays. Cleaning is integrated in the
combined chemical and biological Decontamination Procedure.
Complete servicing consists of three mandatory procedures, to be
performed in the following order: decontamination, maintenance [see
respective Maintenance Procedure*] and calibration [see respective
Adjustment Procedure*].
Parts that are found to have been damaged or to have been attacked
chemically must be replaced with new ones before reassembling the
pipette. Any parts that you remove from the pipette because they
suffered chemical or mechanical damage should be decontaminated
before being discarded.
DD
DD
Decec
ecec
econon
onon
ontaminatamina
taminatamina
tamination Rtion R
tion Rtion R
tion Roomoom
oomoom
oom
The decontamination room must be separate from all other rooms in
the laboratory. For safety reasons, only authorized personnel should
be allowed to enter the decontamination room.
EE
EE
Equipmenquipmen
quipmenquipmen
quipment and Clothingt and Clothing
t and Clothingt and Clothing
t and Clothing
1) Protective clothing, safety glasses, and disposable gloves.
2) Geiger-Müller counter for detecting beta, gamma and X-rays.
3) Benches, one equipped with a safety hood (without filter).
4) Disposable bench covers and safety bags.
5) A safety box (e.g. a lead box).
6) A separate identifiable plastic box for each pipette.
7) For the manual method, small round brushes for cleaning the in-
sides of the tip-holders.
8) For the alternative immersion method: an ultrasonic bath, lattice
boxes and lattice container, plus a thermostatically controlled oven
(50 °C to 60 °C).
End-users mEnd-users m
End-users mEnd-users m
End-users must decust dec
ust decust dec
ust decon-on-
on-on-
on-
taminattaminat
taminattaminat
taminate their pipe their pip
e their pipe their pip
e their pipettett
ettett
etteses
eses
es
beforbefor
beforbefor
before sending them te sending them t
e sending them te sending them t
e sending them too
oo
o
their loctheir loc
their loctheir loc
their local Gilson Sal Gilson S
al Gilson Sal Gilson S
al Gilson Serer
erer
ervicvic
vicvic
vicee
ee
e
CC
CC
Centent
entent
enterer
erer
er
.....
* To be carried out by suitably quali-
fied people only. End-users should
refer to their local distributor for fur-
ther information.
Damaged parts: contact your local
Gilson distributor, who will either
supply you with genuine Gilson user-
level replacements parts or who will
service the pipette for you.

Page 4
Introduction
SS
SS
Solutions folutions f
olutions folutions f
olutions for Dor D
or Dor D
or Decec
ecec
econon
onon
ontaminatamina
taminatamina
taminating and Cleaningting and Cleaning
ting and Cleaningting and Cleaning
ting and Cleaning
Gilson recommends the use of a solution containing a mixture of
chemicals: a detergent for chemical cleaning and a disinfectant for
biological decontamination. For example: Aniospray 41 and Aniosyme
P.L.A. from Anios; if you have difficulty in obtaining either of these solutions,
you may contact Gilson for a list of distributors.
The solutions mentioned above are compatible with pipette materials
and have a wide spectrum of action. Other solutions may be used,
however you must check with your supplier that the solution you use
does not attack any of the following materials: aluminium, stainless
steel, nitrile, ABS (acrylonitrile butadiene styrene), PBT (polybutene
terephtalate), PC (polycarbonate), PE (polyethylene), PEI (polyether
imide), POM (polyoxymethylene), PPS (polyphenylene sulfide), PTFE
(polytetrafluoroethylene), PVDF (polyvinylidene fluoride) and PMP
(polymethylpentene).
PP
PP
Prr
rr
recautionsecautions
ecautionsecautions
ecautions
Decontamination implies that for any person in contact with returned
pipettes there is a risk of contamination. Therefore, systematic
vaccination against hepatitis B of all persons in contact with pipettes is
highly recommended. Because other pathogens could be present,
great care should be taken when handling pipettes.
Use disposable gloves and any other protective clothing that may be
necessary throughout the Decontamination Procedure. Disposable
gloves must be worn at all times during the cleaning and
decontamination procedures.
Follow the regulations in force in your country for handling and
disposing of material that has been exposed to radioactivity. Dispose
of any damaged parts and used water or chemical solutions safely, in
accordance with the regulations in force in your country.
Record the serial number, which is engraved on the body of the
pipette, and identify the boxes used to contain the component parts
of the pipette with this serial number.

Page 5
Procedure for End-users
If your pipettes are used for dispensing radioactive liquids, you must
check each pipette separately for radioactivity.
If a pipette is found to be radioactive, put it in a safety box and pro-
ceed according to the regulations that apply in your country.
If your pipettes are not used for dispensing radioactive liquids or are
found not to be radioactive:
- disassemble the pipettes in accordance with the instructions given
on Page 8.
- decontaminate and dry the pipettes in accordance with the instruc-
tions given on Page 10.

Page 6
Complete Procedure for Gilson Distributors
The complete procedure comprises the following steps:
- checking the outside of the packaging for radioactivity,
- unpacking the pipettes and checking each pipette separately for
radioactivity,
- disassembling the pipettes in accordance with the instructions given
in the Maintenance Procedure,
- decontaminating and drying; cleaning-up the work area,
- transfering the pipettes in their boxes to the repair room for reas-
sembly.
CheckCheck
CheckCheck
Checking the Outside of the Ping the Outside of the P
ing the Outside of the Ping the Outside of the P
ing the Outside of the Packack
ackack
ackage fage f
age fage f
age for Ror R
or Ror R
or Radioacadioac
adioacadioac
adioactivittivit
tivittivit
tivityy
yy
y
1) Place a disposable absorbent cover on the bench, below the safety
hood.
2) Upon receipt, put the package containing the pipette(s) on the
disposable cover, below the safety hood.
3) Before undoing the package, check it for radioactivity (beta,
gamma, and X-rays) using a Geiger-Müller counter
4) If the level of radioactivity exceeds 10 Bq for beta and gamma rays
or 25 Bq for X-rays, store the package in a safety box or dispose of
it.
5) We recommend that if the level of radioactivity is less than or equal
to 10 Bq for beta and gamma rays or 25 Bq for X-rays, you may unpack
the pipette(s). However, if the laws and directives (national and in-
ternational) that apply in your country prescribe different limits, you
should use the lowest limit.
UnpackUnpack
UnpackUnpack
Unpacking the Ping the P
ing the Ping the P
ing the Pipettipett
ipettipett
ipettes and Checkes and Check
es and Checkes and Check
es and Checking fing f
ing fing f
ing for Ror R
or Ror R
or Radioacadioac
adioacadioac
adioactivittivit
tivittivit
tivityy
yy
y
1) Place a disposable absorbent cover on the bench, below the safety
hood.
2) Unpack the pipette(s) and place them on the disposable cover,
below the safety hood. Take out the customer’s shipping docu-
ments before disposing of the packaging.
3) Check the pipettes individually for radioactivity (beta, gamma, and
X-rays) using a Geiger-Müller counter.
4) If the level of radioactivity exceeds 10 Bq for beta and gamma rays
or 25 Bq for X-rays store the pipette in a safety box or dispose of it.
Procedures for storage and disposal must be in accordance with
the laws and directives (national and international) that apply in
your country.
Procedures for storage and disposal
must be in accordance with the laws
and directives (national and interna-
tional) that apply in your country.

Page 7
Complete Procedure for Gilson Distributors
5) We recommend that if the level of radioactivity is less than or equal
to 10 Bq for beta and gamma rays or 25 Bq for X-rays, you may
disassemble the pipette(s). However, if the laws and directives (na-
tional and international) that apply in your country prescribe dif-
ferent limits, you should use whichever of the limits are the lower.

Page 8
Disassembly Procedures
The following are brief summaries of disassembly procedures. We
strongly recommend you to refer to the appropriate User’s guide for more
information. Please refer also to the opposite schematics.
PP
PP
Pipetman P and Pipetman P and P
ipetman P and Pipetman P and P
ipetman P and Pipetman Fipetman F
ipetman Fipetman F
ipetman F
1) Push the tip-ejector button and remove the tip-ejector.
2) Unscrew the connecting nut.
3) Remove the piston assembly from the tip-holder.
4) Remove the seal and the O-ring from the piston.
PP
PP
Pipetman 8x200ipetman 8x200
ipetman 8x200ipetman 8x200
ipetman 8x200
1) Unscrew the connecting nut.
2) Undo the 6 screws of the liquid end box.
3) Lift out the nozzle assemblies.
4) Remove the pistons from the nozzles.
5) Wipe the silicon grease off the 8 pistons.
MM
MM
Micricr
icricr
icromanoman
omanoman
oman
1) Unscrew the capillary-holder.
2) Remove the clamp assembly and return spring from the capillary-holder.
DistrDistr
DistrDistr
Distrimaniman
imaniman
iman
Disassemble the piston retaining assembly.
1) Hold your Distriman in the horizontal position with the red flag in front
of you.
2) Hold the filling knob at its lower position using the thumb of on hand.
3) Press lightly on the white locking tab with a screwdriver.
4) Move the filling knob toward the upper position to separate it from
the assembly.
5) Pull the assembly out of the Distriman’s neck, taking care not to lose
the small red flag.
PP
PP
Pipetman Ultripetman Ultr
ipetman Ultripetman Ultr
ipetman Ultra single channela single channel
a single channela single channel
a single channel
1) Push the ejection button and remove the tip-ejector by rotating it
gently counterclockwise.
2) Unscrew both parts of tip-holder (remove also O-ring and seal).
3) Set the counter to zero.
4) Unscrew the connecting nut.
5) Pull on the spring guide to remove the piston from the body.
PP
PP
Pipetman Ultripetman Ultr
ipetman Ultripetman Ultr
ipetman Ultra Multichannela Multichannel
a Multichannela Multichannel
a Multichannel
1) Push the tip-ejector button and remove the ejector clip by rotating it
gently counterclockwise.
2) Set the counter to zero.
It is recommended that you
replace the seal and O-ring with
new ones. Your local distributor
is able to supply you, genuine
Gilson spare parts and to advise
you about the way to service
your pipette.

Page 9
Disassembly Procedures
Pipetman Ultra - You may wipe or immerse the
following parts:
- tip ejector,
- tip-holder (upper and lower parts),
- piston spring and spring guide,
- connecting nut.
Pipetman Ultra Multichannel - You may wipe or immerse the
entire head or individually the following parts:
- piston tray,
- cover-ejector,
- tip-holder,
- piston and spring,
- connecting nut,
- clips.
Wipe only, Do not immerse.
3) Unscrew the connecting nut to separate the head from the body.
4) Take the head and remove the two screws and pull the cover-ejector
away.
5) Remove the wire clip(s), and push awards (the piston cover) to flip
upwards it.
6) Remove the tip holders and pistons.
7) Pull each piston out of each tip holder.
Upper Part (Body)
Lower Part
Pipetman P/F
Pipetman Ultra Pipetman Ultra
Multichannel

Page 10
Decontamination Methods
Two methods are described below: the manual method, which can
be used with any part of the pipette, and the immersion method,
which is only for the parts that can be removed from the upper part
(body) of the pipette. TT
TT
The body of the pipetthe body of the pipett
he body of the pipetthe body of the pipett
he body of the pipette must not bee must not be
e must not bee must not be
e must not be
immersed in animmersed in an
immersed in animmersed in an
immersed in any liquidy liquid
y liquidy liquid
y liquid.....
The manual method is quicker to perform than the immersion
method. If an ultrasonic bath is not available you can still immerse
the specified parts after cleaning them manually.
When the decontamination process is finished, you should disinfect
the empty boxes, the brushes (manual method), the ultrasonic bath
(immersion method) and the bench with a 10 % v/v sodium hypochlo-
rite solution; then rinse with water.
MM
MM
Manual Manual M
anual Manual M
anual Methodethod
ethodethod
ethod
1) Wipe the body of the pipette with a tissue soaked in Aniospray 41.
2) Place the body of the pipette in a clean box.
3) Spray the other disassembled pipette parts with Aniospray 41.
4) Clean inside the tip-holder with small (soft plastic) brushes (both
parts).
5) Dispose of the O-rings and/or piston seals and any damaged parts
(after cleaning them).
6) Place the remaining clean parts from one pipette in a separate box
(identified by serial number for cross-referencing with upper part
(body).
7) Wipe with a tissue soaked in distilled water, then leave the pipettes
to dry (about 15 minutes).
II
II
Immersion Mmmersion M
mmersion Mmmersion M
mmersion Methodethod
ethodethod
ethod
1) Fill the ultrasonic bath with hot water, then add the Aniosyme P.L.A
to a concentration of 0.5 % (refer to the instructions given by Anios).
2) Place the component parts from an individual pipette in a separate
lattice-box (identified with that pipette’s serial number). Put any
damaged parts from the batch of pipettes in another box and wash
them before disposing of them.
3) Place all of the lattice boxes in a lattice container.
4) Immerse the lattice container in the ultrasonic bath for 15 minutes.
5) Remove the lattice container (containing the lattice boxes and
pipette parts) from the ultrasonic bath.
Note: You may find it difficult to clean
inside the lower part of the smaller
pipettes (e.g. P2 or U2), in which case
you should either replace the part with
a new one or use an ultrasonic bath
(immersion method).
Liquid must not enter the upper
part (body) of the pipette.

Page 11
Decontamination Methods
6) Rinse the lattice container (containing the lattice boxes and pipette
parts) thoroughly with warm water (60 °C maximum).
7) Place the component parts (still in their individual lattice-boxes)
in an oven for about 30 minutes at 50 °C to 60 °C.
8) Wipe the upper part (body) of each pipette with a tissue soaked
with Aniospray 41, then wipe it with a tissue soaked with distilled
water.
9) Reunite the upper part (body) of the pipette with its component
parts by placing it in the lattice-box having the same serial number.
DD
DD
Damaged Pamaged P
amaged Pamaged P
amaged Parar
arar
arts:ts:
ts:ts:
ts:
CC
CC
Contacontac
ontacontac
ontact yt y
t yt y
t your Gilson Dour Gilson D
our Gilson Dour Gilson D
our Gilson Distributistribut
istributistribut
istributor tor t
or tor t
or to obtain geno obtain gen
o obtain geno obtain gen
o obtain genuine Gilson sparuine Gilson spar
uine Gilson sparuine Gilson spar
uine Gilson sparee
ee
e
parpar
parpar
parts for ants for an
ts for ants for an
ts for any damaged pary damaged par
y damaged pary damaged par
y damaged partsts
tsts
ts. Y. Y
. Y. Y
. Your distributour distribut
our distributour distribut
our distributor will also be able tor will also be able t
or will also be able tor will also be able t
or will also be able too
oo
o
manage the sermanage the ser
manage the sermanage the ser
manage the servicing of yvicing of y
vicing of yvicing of y
vicing of your pipour pip
our pipour pip
our pipettett
ettett
etteses
eses
es.....

Page 12
Associated Documents
DD
DD
Documenocumen
ocumenocumen
ocumentsts
tsts
ts Gilson RGilson R
Gilson RGilson R
Gilson Refef
efef
eferer
erer
erencenc
encenc
encee
ee
e
Pipetman Ultra User’s Guide LT801441
Pipetman P User’s Guide LT801117
Pipetman F User’s Guide LT801118
Pipetman 8X200 User’s Guide LT801236
Pipetman Ultra Multichannel LT801462
Microman User’s Guide LT801367
Distriman User’s Guide LT801285
Verification Procedure for Accuracy and Precision LT802292

Page 13
Notes

World Wide Web : www.gilson.com
E-mail : [email protected], [email protected], [email protected]
World Headquarters
Gilson, Inc.
3000 W. Beltline Hwy., P.O. Box 620027, Middleton, WI 53562-0027, USA
Telephone: [phone] or (1) 608-836-1551 • Fax: [phone]
Gilson SAS
19 avenue des Entrepreneurs, B.P. 145
95400 Villiers-le-Bel, France
Telephone: [phone] • Fax: [phone]