Gilson Pipetman Ultra U2 User manual

EN ENGLISH User's Guide
PIPETMAN®ultra

2
PIPETMAN®ultra
1  INTRODUCTION
Congratulations on acquiring your new Pipetman
Ultra. It is a quality product from Gilson, which is
fullyISO8655compliant,CElabeled(conformstothe
EC directives on in vitro diagnostic medical devices,
electromagneticcompatibilityandsafety).Enjoythe
following features.
Easy of use:
- Continuously-adjustable parallax-free electronic
display for easy volume setting,
Contents Page
1  INTRODUCTION 2
2  PARTS CHECK LIST 3
3  DESCRIPTION 4
4  SWITCHING ON 5
5  VOLUME INDICATOR  LCDWINDOW 6
6  SETTING THEVOLUME 7
7  GILSON DIAMOND®TIPS 8
8  PIPETTING 10
9  PERSONALIZINGYOUR PIPETTE 12
10  GLP FEATURES 14
11  TROUBLESHOOTING 15
12  MAINTENANCE 16
13  CLEANING AND DECONTAMINATION 26
14  LEAKTEST 28
15  USER ADJUSTMENT 29
16  SPECIFICATIONS 31
17  EQUIPMENT DISPOSAL 33
18  SPARE PARTS 34
APPENDIX  RESET PROCEDURE 40
EC DECLARATION OF CONFORMITY 41

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- Flashing indicators if volume set is out of range,
- Ergonomic design, maximum comfort for left or
right-hand users,
- Low tip-ejection force, light, balanced,
- Simple to maintain and adjust,
- Other GLP features.
Performance:
- Accurate and precise,
- Unbeatable results with Gilson Diamond Tips,
- Easy and secure tip loading from Gilson Tipacks,
- New auto-shut off system to maximize battery
life.
Applications:
- For any type of lab.
- Suitable for most liquids.
- Molecular studies, kinetic studies, DNA sequenc-
ing, enzyme-assays, and the measurement
and transfer of general aqueous solutions.
2  PARTS CHECK LIST
Just take a moment to verify that the following items
are present:
- Pipetman Ultra pipette,
- Certificate of conformity (including bar-code
sticker),
- User's Guide,
- Identity tags (5 different colors),
- Safety bag,
- Recalibration (user adjustment) tool,
-
Tip-holderreassemblytool(Multichannelmodels
only),
- Lubricant tube (except for U2 and U10),
- Tip ejector extension (U2 and U10 only),
- Sample pack of tips.

4
PIPETMAN®ultra
3  DESCRIPTION
See below the legends to the opposite figure.
Push-buttonforswitchingon,settingthevolume,
aspirating,anddispensing(buttoniscolorcoded
with the volume range printed on the top).
Ergonomic stabilizer, to make the pipette more
comfortable to use, and to reduce fatigue.
Thumbwheel, to set and lock the volume.
Tip-ejector button, can be positioned for left or
right-handed operation.
a
Liquid Crystal Display (LCD) window for volume-
setting and status indicators.
b Identity-tag window (see “GLP features”).
Connecting nut.
Tip-ejector stroke adjustment-wheel.
Tip-holders–removableforcleaningandservicing .
Ejector: removable to access piston assemblies
and tip-holders.
Table - Operating Ranges
Model Reference Range Color code
U2 F21021 0.2 - 2 μL Orange
U10 F21022 1 - 10 μL Red
U20 F21023 2 - 20 μL Pale yellow
U100 F21024 20 - 100 μL Peach
U200 F21025 20 - 200 μL Yellow
U1000 F21026 200 - 1000 μL Blue
U5000 F21027 1 - 5 mL Purple
U10ml F21028 1 - 10 mL Clear blue
8x20 F21040 1 μL to 20 μL Pale yellow
12x20 F21041 1 μL to 20 μL Pale yellow
8x300 F21042 20 μL to 300 μL Green
12x300 F21043 20 μL to 300 μL Green
Pipetman Ultra specifications are only valid with the use
of Diamond tips.

5
Body
(Handle)
Volumetric
module
(lower part)
4  SWITCHING ON
Press the push-button to the second stop (purge)
to switch on the pipette. The information displayed
in the LCD window depends on the status of the
pipette and the volume currently set (see below).
When the volume is locked , Pipetman Ultra turns itself
off after 3 min of inactivity – just press the push-button to
the second stop (or unlock the thumbwheel) to reactivate
the pipette.

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PIPETMAN®ultra
If the volume is unlocked for 15 sec, you will see
LOCKED/UNLOCKED flashing
during 10 min to warn you to
lock the thumbwheel.
If you havenot lockedthepipette,Pipetman
Ultraturnsitselfoffafter10minofinactivity.
Justlockthethumbwheel(volumesetting)and
“Init 1” will appear.
Follow the instruc-
tions of Appendix to
reset the pipette.
5  VOLUME INDICATOR  LCD WINDOW
What you normally see...
Volume setting
Units (μL or mL)
Volume status (locked or
unlocked)
What may appear...
Calibration status:“RECAL”this means that your
pipette has been readjusted.
Battery status: failing–“LOWBATT”here,orfailed
– “BATT” in place of volume setting.
Electronic counters:
Holding down the push button to the second stop
for 5 seconds, successively displays counters at the
right-hand side of an otherwise blank screen. Are
displayed in order:
a) Cycle counter since the last volume setting
– the counter indicates the
number of pipetting cycles
(max. 199); for example 126
cycles.
b) Cycle counter since the pipette was made – the
counter indicates the number of pipetting cycles
|
00
LOW BAT UNLOCKED RECAL
μl


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(maximum 999,999); for
example 285,396 cycles.
c) Readjustment counter since
the pipette was made – the
counter indicates the num-
ber of times the pipette has
been readjusted (maximum
99); for example 03 readjustment operations.
Refer to “Troubleshooting” for possible error messages.
6  SETTING THE VOLUME
You must perform the following steps:
Switch on: press the push-button to the second
stop. LCD shows the volume currently set.
Unlock: push the thumbwheel upwards. LCD
shows UNLOCKED (also visible on the thumb-
wheel).
Set the volume: rotate the thumbwheel or the
push-button (clockwise to decrease the volume,
counterclockwise to increase):
- when decreasing the volume, reach the
required setting slowly, making sure not to
overshoot the mark.
- whenincreasingthevolume,passtherequired
value by 1/3 of a turn, then decrease the vol-
ume slowly, making sure not to overshoot the
mark.
Lock the volume: push the thumbwheel down-
wards. LCD shows LOCKED.
03
RECAL
To unlock
To lock
Thumbwheel
LCD
|00
UNLOCKED
μl


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PIPETMAN®ultra
Toreducebatteryconsumption,lockthevolume
before storing Pipetman Ultra.
• You are strongly advised to lock the volume, to avoid ac-
cidentally changing the setting when pipetting.
• Theunitsindicatorflashes,ifyousetavolumebelow90%of
theminimumrecommended;youcandispenseanaspirated
volume below the minimum recommended. However, in
this case, the specifications cannot be guaranteed.
• The volume indicator flashes, if you try to set a volume
abovethe maximumpermitted;youmustnot try topipette
a volume that is greater than the maximum permitted.
7  GILSON DIAMOND TIPS
GilsonDiamondTipsaremadetothehighestspecifica-
tions; strict quality control is maintained throughout
the manufacturing process.
Diamond Tips are used to calibrate
Pipetman Ultra, therefore for opti-
mumperformance,youarestrongly
advised to use Gilson’s Diamond
Tips with your pipette. Diamond
Tips have the Gilson logo engraved
on their collar, ensuring that you
have a genuine Gilson product.
To ensure accuracy and precision, Gilson’s Quality
Assurance System focuses on the following critical
parameters.
• Diamond Tips are made from pure polypropylene
(virgin, metal-free, to avoid the possibility of con-
tamination). They are available sterilized and with
filters.
• They may be autoclaved at 121 °C for 20 minutes
at 0.1 MPa.
• Gilson's sterilized DiamondTips are certified free of
detectable RNases, DNases, DNA, RNA, pyrogens,
and trace metal.
• Optimized shape (revised collar for optimum seal-
ing, thin walls, and fine point), making them easier

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to mount, more flexible, with no vortexing, and
improved precision.
• Mold and cavity references are marked on the col-
lar, ensuring the traceability for quality assurance
purposes, batch numbers appear on all packages
(bags and boxes).
• They form an air-tight seal with the tip-holder,
preventing the leaks that cause poor accuracy and
a lack of precision.
Tipack - Rocky Rack
Diamond Tips are best
fitted from a Rocky Rack
(patented). Rocky Rack is
the dome-shaped part of
the pack that contains the
tips. Rocky Rack makes it
easy to securely fit the tips
to a multichannel pipette,
ensuring an airtight seal
onallchannelswithoutthe
need to use undue pres-
sure or to touch the tips.
Table - Tips to use for best results
Pipette Diamond Tips Volume (Range)
U2 D10, DL10 0.2 μL to 2 μL
U10 D10, DL10 1 μL to 10 μL
U20 D200 2 μL to 20 μL
8X20 DL10, D200 1 μL to 20 μL
12X20 DL10, D200 1 μL to 20 μL
U100 D200 20 μL to 100 μL
U200 D200 20 μL to 200 μL
8X300 D200, D300 20 μL to 300 μL
12X300 D200, D300 20 μL to 300 μL
U1000 D1000 200 μL to 1 mL
U5000 D5000 1 mL to 5 mL
U10 ml D10 ml 1 mL to 10 mL

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PIPETMAN®ultra
8  PIPETTING
Operating procedure
1) Optionally,forUltra Multichannelsettheheadto
any position that is comfortable and practicable
for your application. To do this, simply turn the
head to the desired position.
2) Fit new Diamond Tips.
You must fit tips to your pipette before aspirating and
dispensing any liquid. Plastic tips are for a single ap-
plication – they must not be cleaned for reuse.
3) Pre-rinse the tips.
Some liquids (e.g. protein-containing solutions
andorganicsolvents)canleaveafilm of liquidon
the inside the wall of the tip; pre-rinse the tip to
minimize any errors that may be related to this
phenomenon. Pre-rinsing consists of aspirating
the first volume of liquid and then dispensing it
back into the same vessels (or to waste). Subse-
quent volumes that you pipette will have levels
of accuracy and precision within specifications.
4) Aspirate.
Press the push-
button to the
first stop (this
corresponds to
the set volume of
liquid).
Hold the pipette
vertically (± 20°)
and immerse the
tip in the liquid (see
table for immersion
depth).
Release the push-button slowly and smoothly
(to top position) to aspirate the set volume of
liquid.
Wait onesecondthenwithdrawthepipette-tip
from the liquid.
Model Immersion Wait Time
Depth (mm) (seconds)
U2 1 1
U10 1 1
U20 2-3 1
8x20, 12x20 2-3 1
U100 2-4 1
U200 2-4 1
8x300, 12x300 2-3 1
U1000 2-4 2-3
U5000 3-6 4-5
U10 ml 5-7 4-5
Table - Immersion Depth
and Wait Time