Hanna Instruments HI 96812 User manual

HI96812
Refractometer for Sucrose
in Wine and Grape Products
Measurements
INSTRUCTION MANUAL

Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using
this instrument.
This manual will provide you with the necessary information
for correct use of this instrument, as well as a precise idea
of its versatility.
If you need additional technical information, do not hesitate
to e‑mail us at [email protected] or view our worldwide
contact list at www.hannainst.com.
Dear
Customer,
All rights are reserved. Reproduction in whole or in part is prohibited
without the written consent of the copyright owner, Hanna Instruments
Inc., Woonsocket, Rhode Island, 02895, USA.

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TABLE OF CONTENTS
Preliminary Examination .......................................................
General Description...............................................................
Specifications.......................................................................
Principle of Operation...........................................................
Functional Description...........................................................
Display Elements..................................................................
Measurement Guidelines .......................................................
Calibration Procedure............................................................
Measurement Procedure........................................................
Making a Standard % Brix Solution ......................................
To Change Temperature Unit .................................................
Battery Replacement.............................................................
Error Messages.....................................................................
Accessories...........................................................................
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Remove the instrument from the packing materials and examine carefully
to ensure no damage has occurred during shipping. If any damage has
occurred, contact your local Hanna Instruments Office.
Each HI96812 instrument is supplied with:
• 9 V battery
• Instruction manual
• Plastic pipette
• Quality Certificate
Note: Save all packing material until you are sure that the instrument
functions correctly. A defective instrument must be returned in its
original packing.
PRELIMINARY EXAMINATION

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The HI96812 Digital Wine Refractometer is rugged portable, water resistant
devices that benefit from Hanna Instruments’ years of experience as a
manufacturer of analytical instruments. Hanna Instruments offers five related
wine refractometers to fill the differing requirements found in the industry.
All are equally valid ways to measure the sugar content of grape or must
samples in the field or winery.
The HI96812 is optical instruments that are based on the measurement of
the refractive index of a solution. The measurement of refractive index is
simple and quick and provides the vintner a standard accepted method for
sugar content analysis. Samples are measured after a simple user calibration
with deionized or distilled water. Within seconds the instrument measures
the refractive index of the grape. These digital refractometers eliminate the
uncertainty associated with mechanical refractometers and are easily por‑
table for measurements in the field. Temperature (in °C or °F) is displayed
simultaneously with the measurement on the large dual level display along
with icons for Low Power and other helpful message codes.
Key features include:
• Dual‑level LCD
• Automatic Temperature Compensation (ATC)
• Easy setup and storage
• Battery operation with Low Power indicator (BEPS)
• Automatically turns off after 3 minutes of non‑use
• Single‑point calibration with distilled or deionized water
• Waterproof models offers IP65 waterproof protection
• Quick,preciseresultsreadingsaredisplayedinapproximately1.5 seconds
• Small sample size as small as 2 metric drops.
GENERAL DESCRIPTION

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SPECIFICATIONS
Range 0.0 to 28.0 °Baumé/ 0.0 to 80.0 °C
(32.0 to 176.0 °F)
Resolution 0.1 °Baumé/ 0.1 °C (0.1 °F)
Accuracy ± 0.1 °Baumé /
± 0.3 °C (±0.5 °F)
Temperature Compensation Automatic between 10.0 and 40.0 °C
(50.0 ‑ 104.0 °F)
Measurement Time Approximately 1.5 seconds
Minimum Sample Volume 100 µL (cover prism totally)
Light Source Yellow LED
Sample Cell Stainless Steel ring and flint glass prism
Case Material ABS
Enclosure Rating IP65
Battery Type / Life 9V / 5000 readings
Auto‑Off After 3 minutes of non‑use
Dimensions 192 x 102 x 69 mm (7.6 x 4.1 x 2.7”)
Mass 350 g (12.3 oz)

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The °Baumé scale is based on density and was originally designed to mea‑
sure the mass of sodium chloride in water. °Baumé is used in wine making
to measure the sugar in must. The HI96812 converts the %Brix reading
to °Baumé based on the table found in the Official Methods of Analysis
of AOAC International, 18th Edition. 1 °Baumé is approximately equal to
1.8 %Brix, and 1 °Baumé is roughly equivalent to 1 % alcohol when the
wine is fully fermented.
In the HI96812, light from an LED passes through a prism in contact with
the sample. An image sensor determines the critical angle at which the light
is no longer refracted through the sample.
The °Baumé determination is made by measuring the refractive index of a
solution. Refractive Index is an optical characteristic of a substance and the
number of dissolved particles in it. Refractive Index is defined as the ratio of
the speed of light in empty space to the speed of light in the substance. A
result of this property is that light will “bend”, or change direction, when it
travelsthroughasubstanceofdifferentrefractiveindex.Thisiscalledrefraction.
When passing from a material with a higher to lower refractive index, there
is a critical angle at which an incoming beam of light can no longer refract,
but will instead be reflected off the interface. The critical angle can be used
to easily calculate the refractive index according to the equation:
sin (θcritical) = n2/ n1
Where n2is the refractive index of the lower‑density medium; n1is the
refractive index of the higher‑density medium.
PRINCIPLE OF OPERATION

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FUNCTIONAL DESCRIPTION
1. Liquid Crystal Display (LCD)
2. Secondary Display
3. Primary Display
4. READ Key (User Measurement)
5. ZERO Key (User Calibration)
6. ON/OFF
7. Stainless Steel Sample Well and Prism
TOP VIEW
8. Battery Cover
9. Battery Compartment
BOTTOM

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1. Battery (blinks when low battery condition detected)
2. Primary Display (displays measurement and error messages)
3. Measurement in Progress Tag
4. SETUP: Factory Calibration Tag
5. CAL: Calibration Tag
6. Measurement Units
7. Automatic Temperature Compensation
(blinks when temperature exceeds 10.0‑40.0 °C /50.0‑104.0 °F range)
8. Temperature Units
9. Secondary Display (displays temperature measurements; when
blinking, temperature has exceeded operation range: 0.0‑80.0 °C /
32.0‑176.0 °F)
DISPLAY ELEMENTS

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• Handle instrument carefully. Do not drop.
• Do not immerse instrument under water.
• Do not spray water to any part of instrument except the “sample well”
located over the prism.
• The instrument is intended to measure Wine and Grape products. Do not
expose instrument or prism to solvents that will damage it. This includes
most organic solvents and extremely hot or cold solutions.
• Particulate matter in a sample may scratch the prism. Absorb sample
with a soft tissue and rinse sample well with deionized or distilled water
between samples.
• Use plastic pipettes to transfer all solutions. Do not use metallic tools
such as needles, spoons or tweezers as these will scratch the prism.
• To reduce the effects of evaporation or absorption of water when taking
readings over a period of time, the prism and sample well can be covered
with plastic wrap.
MEASUREMENT GUIDELINES