PerkinElmer Caliper IVIS Spectrum User manual

IVIS Spectrum
In Vivo and In Vitro
3D Optical Imaging
Bioluminescence and Fluorescence
Spectral Unmixing
Transmission and Reflectance Imaging
®
Hardware Manual
March 2012

© 2007-2012 Caliper Corporation. All rights reserved.
121450_Rev01
Caliper LIfe Sciences
68 Elm Street
Hopkinton, MA 01748
Main Phone: [phone]
Fax: [phone]
E-mail: [email protected]
www.caliperls.com
Discovery in the Living Organism, IVIS Imaging System and Living Image are either registered trademarks or
trademarks of Xenogen Corporation. The names of companies and products mentioned herein may be the
trademarks of their respective owners. Apple, Macintosh and QuickTime are registered trademarks of Apple
Computer, Inc. Microsoft, PowerPoint and Windows are either registered trademarks or trademarks of Microsoft
Corporation in the United States and/or other countries. Adobe and Illustrator are either registered trademarks or
trademarks of Adobe Systems Incorporated in the United States and/or other countries.

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IVIS® Spectrum Hardware Manual
Contents
1 Welcome . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.1 About the IVIS Spectrum Imaging System . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2 IVIS Imaging Systems Technology Support . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
2 Important Safety Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.1 Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.2 Instrument Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.3 Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.4 Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.5 Fluorescence Light Source Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.6 Environmental Considerations for the System Components . . . . . . . . . . . . . . . . . . . 7
2.7 Power Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.8 Cleaning or Moving the System Components . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.9 Other Equipment, Chemicals, or Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.10 Servicing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3 Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.1 Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.2 Burn Hazard & Eye Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.3 Mechanical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.4 Chemical & Biological Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.5 Panels, Covers & Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4 Legal Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.1 Limited Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.2 Patents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4.3 Trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.1 Electrical Power Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.2 CCD Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.3 Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.4 Fluorescence Imaging Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.5 Environmental Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Contents
ii
5.6 Weights & Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
6IVIS
®Spectrum Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
6.1 CCD Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6.2 ThermoCube Chiller Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6.3 Imaging Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.4 Optical Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
6.5 Specimen Warming System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
6.6 Laser/Galvanometer System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
6.7 Crash Avoidance System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
6.8 Base Enclosure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
6.9 Acquisition Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.10 Accessory Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.11 High Reflectance Hemisphere . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
7 Operating the IVIS®Spectrum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
7.1 Restarting the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
7.2 Gas Plumbing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
7.3 Door Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
7.4 Centering a Subject in the Field of View . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
7.5 Fluorescent Imaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
7.6 Imaging Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
7.7 System Shut Down Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
8 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
8.1 Measured Temperature Does Not Equal the Demand Temperature . . . . . . . . . . . . . . . 45
8.2 Photographic Image Is Unacceptable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
8.3 Luminescent Image Is Unacceptable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
8.4 No Image Is Produced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
8.5 Hardware Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
8.6 Accessing the Area Under the Imaging Platform . . . . . . . . . . . . . . . . . . . . . . . . 50
9 Care & Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
9.1 Cleaning the IVIS Spectrum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
9.2 Cleaning the Lens Protection Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
9.3 Changing the Emission Filters Used in Fluorescence Imaging . . . . . . . . . . . . . . . . . 53

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IVIS® Spectrum Hardware Manual
9.4 Moving the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
10 Servicing & Maintaining the ThermoCube Chiller Unit . . . . . . . . . . . . . . . . 57
10.1 Required Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
10.2 Turning Off Electric Power to the Main Console . . . . . . . . . . . . . . . . . . . . . . . 57
10.3 Removing the Console Rear Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
10.4 Sliding the Tray Out . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
10.5 Filling the ThermoCube Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
10.6 Reassembling the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
10.7 Restarting the IVIS Spectrum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Appendix A Spare Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Contents
iv

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IVIS® Spectrum Hardware Manual
1 Welcome
About the IVIS Spectrum Imaging System . . . . . . . . . . . . . . . . 1
IVIS Imaging Systems Technology Support . . . . . . . . . . . . . . . 3
1.1 About the IVIS Spectrum Imaging System
The IVIS®Spectrum Imaging System is a high-sensitivity, low noise, in vivo
imaging technology platform that enables noninvasive visualization and
tracking of cellular and genetic activity within a living organism in real time
(Figure 1.1). The system provides both bioluminescence and fluorescence
imaging capability.
For fluorescence imaging, the instrument can operate in reflectance or
transillumination mode. Filtered light from a broad-band lamp provides the
excitation source in both modes. In the reflectance mode, light is delivered to
four reflectors that are located on the ceiling of the imaging chamber. In the
transillumination mode, the excitation light is delivered to an x-y translation
assembly under the stage and focused to a 2 mm diameter beam that can be
directed to a particular location on the underside of the animal subject. The
system includes ten excitation and 18 emission filters that enable spectral
scanning of reporters over the range from 480-850 nm.
3D Tomography The system also includes a structured light projector that enables reconstruction
of the surface topography. The 3D location and concentration of fluorescent
sources can be computed from structured light and transillumination fluorescent
images. The 3D location and strength of luminescent sources is computed from
structured light and luminescent image data.

1. Welcome
2
The IVIS Spectrum is an integrated imaging system that includes a:
•CCD camera mounted on a light-tight imaging chamber
•Camera power supply
•Thermoelectric cooling unit
•Fluorescence light source
•Windows®-based computer system for data acquisition and analysis.
The sound-limiting enclosure beneath the imaging chamber contains the
thermoelectric cooling unit, camera power supply, and other imaging chamber
functions. All components except the computer are integrated into a single,
moveable chassis.
This manual explains how to operate and maintain the equipment, and provides
guidelines for obtaining the best bioluminescent or fluorescent images. Before
using the IVIS Spectrum, please read this manual carefully to obtain safe,
optimum performance and a maximum service life from the unit. For
instructions on using the system software, please see the Living Image®
Software User’s Manual.
If you have questions regarding this manual or the IVIS Imaging System, please
call IVIS Imaging Systems Technology Support.
Figure 1.1 IVIS®Spectrum Imaging System
High resolution monitor
XWS-260 Workstation
CCD
camera
Imaging
chamber
Compartment that contains
the ThermoCube chiller unit
and camera power supply
Acquisition
computer
XGI-8 Gas
Anesthesia
System

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IVIS® Spectrum Hardware Manual
1.2 IVIS Imaging Systems Technology Support
For technical support, please contact Caliper at:
Telephone [phone] (US)
1.508.435.9500
E-mail [email protected]
Fax [phone]
Mail Caliper Life Sciences
US Corporate Headquarters
68 Elm Street
Hopkinton, MA 01748

1. Welcome
4
[This page intentionally blank.]

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IVIS® Spectrum Hardware Manual
2 Important Safety Instructions
Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Instrument Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Environmental Considerations for the System Components . . . . . . 7
Power Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Cleaning or Moving the System Components . . . . . . . . . . . . . . 9
Other Equipment, Chemicals, or Supplies . . . . . . . . . . . . . . . . 9
Servicing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.1 Definitions
When you see the caution, warning, or voltage symbol, pay particular attention
to the safety information presented. Observance of safety precautions will help
avoid actions that could damage or adversely affect the performance of the IVIS
Spectrum. If the equipment is used in a manner not specified in this manual, the
protection provided by the equipment may be impaired.
CAUTION
!
CAUTION! This symbol indicates a potentially hazardous situation
which, if not avoided, may result in minor or moderate injury and/or
mechanical damage. It is also used to alert against unsafe practices. It
reminds you that all safety instructions should be read and understood
before installation, operation, maintenance, or repair of this instrument.
WARNING
!
WARNING! Used when an action or condition may potentially cause serious
personal injury or loss of life. Mechanical damage may also result.
VOLTAGE
VOLTAGE! Indicates high voltage or risk of electric shock.

2. Important Safety Instructions
6
2.2 Instrument Labels
Pay careful attention to the labels on the instrument.
2.3 Instructions
Read Instructions Read and understand all the safety and operating instructions before you install,
operate, or perform maintenance on this product. Make sure that you fully
understand the following safety instructions, warnings, and disclaimers before
proceeding to the rest of the manual.
Retain Instructions Retain the safety and operating instructions for future reference.
Follow Instructions Follow all operating and handling instructions. Failure to follow operating or
handling instructions may void any warranty covering this product.
Heed Warnings Abide by all warnings on the product and in the operating instructions. Failure
to adhere to warnings or safety precautions may void any warranty covering this
product.
Label Description
Laser caution label
Note: The actual warning
label mounted on the laser
module at the service
access inside the unit uses
non-fluorescing colors and
materials.
The Caliper IVIS®Spectrum Imaging Instrument contains a Class 3R Laser module.
The instrument itself is classified as a Class 1 device because the user is safely
protected from the laser beam. The potential hazard of exposure to service
personnel is clearly indicated with a warning label per IEC 60825 and CDRH
requirements.
Read manual label
Where located on the IVIS system, the read manual label indicates that important
information about the labeled area can be found in the IVIS Spectrum Hardware
Manual.
Pinch hazard label
Where located on the IVIS system, the pinch hazard label indicates the location of
a potential pinch point.

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IVIS® Spectrum Hardware Manual
2.4 Laser Safety
The IVIS Spectrum is equipped with Class 3R laser (532 nm (green) laser
source, 5 mW maximum continuous (CW), which is suitably embedded and
limited in power to less than 1mW where accessible in the imaging
compartment behind the front door.
WARNING
!
WARNING! A laser beam can be harmful to the eyes. ABSOLUTELY DO
NOT look directly into the laser beam when the laser is turned on. Be aware
that the laser light can be easily reflected off of shiny surfaces and that the
reflected light can be dangerous. When the laser is on, remove all watches
and jewelry before placing hands in the imaging chamber.
2.5 Fluorescence Light Source Safety
WARNING
!
WARNING! The IVIS Spectrum is equipped with a strong bottom illumination
fluorescence light source. Do not stare directly into this source.
2.6 Environmental Considerations for the System Components
Location for the IVIS®
Spectrum
Before the IVIS®Spectrum is installed, consider the proper environment for the
components.
Install the equipment in an environment where:
•The temperature does not change or fluctuate widely. Choose an
environment where the temperature is maintained between 15-25°C (59-
78°F).
•The humidity does not exceed 80%.
•No strong electric or magnetic fields exist.
•No vibrations are present.
•No corrosive gases are present.
•High amounts of dust are not present.
•No open flame is present.
•There is sufficient space behind the IVIS Spectrum equipment. A
minimum space of six inches from the flat surface of the rear panel should
be provided behind the IVIS Spectrum to provide unobstructed air flow
and access to the main power on/off switch.
•The floor is level and structurally sound.
Heat The system should be situated away from heat sources such as open flames,
radiators, heat registers, stoves, and other heat-generating electrical equipment.

2. Important Safety Instructions
8
Water & Moisture
VOLTAGE
VOLTAGE! Do not use this product near water (for example, near a sink or
wet room) due to risk of electric shock, electrical damage, and/or failure.
Laboratory Space &
Ventilation Requirements
The IVIS®Spectrum requires a minimum of 100 square feet (9 square meters)
of laboratory space for adequate ventilation. At least of six inches of space is
required behind the rear flat panel to provide unobstructed air flow.
Allocate an area at least 36 inches wide and 51 inches deep for the imaging
system console to allow the door to be fully opened and provide the
recommended air space behind the instrument.
An additional area that is 48 inches wide should be provided (preferably on the
right hand side) for a table or cart to hold the computer and provide a work
surface.
2.7 Power Considerations
Power Sources The IVIS®Spectrum is configured for the voltage requirements of the
installation locality that was specified at the time of order. If the system is
moved to another area, check to make sure that the same voltage requirements
exist.
An IVIS Spectrum that operates on 120VAC requires a dedicated 20 Amp
circuit that is not shared with other loads except the computer and the optional
XGI-8 Gas Anesthesia System. Make sure that all equipment is plugged into a
properly grounded AC supply.
The computer should share the same circuit to avoid ground loops. Never use
auxiliary power outlets on other equipment to supply the IVIS Spectrum. Since
the system contains internal surge protection, it must be powered directly from
the main electrical supply wall socket. Use the surge protector provided by
Caliper for the computer and other accessories.
An IVIS Spectrum configured for 230 VAC requires a dedicated minimum 10
Amp circuit subject to the same conditions noted above. For more details on the
operating requirements, see Electrical Power Requirements, page 21.
VOLTAGE
VOLTAGE! The IVIS Spectrum can operate at multiple voltages (100, 120,
220, 240 VAC); however, you are not permitted to change the input voltage
to any of the system components. Several internal modifications are required
for voltage change. If the operating voltage must be changed, contact IVIS
Imaging Systems Technology Support.
Power Cord Protection Power supply cords should be routed so that they are unlikely to be walked on
or pinched by items placed upon or against them. Pay close attention to
receptacles and to points of connection between cords and equipment.
Lightning & Power Line
Surges
The IVIS®Spectrum is supplied with an internal surge protector that is not
customer serviceable. Contact IVIS Imaging Systems Technology Support
regarding equipment servicing.

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IVIS® Spectrum Hardware Manual
A separate surge protector is provided for the computer and other accessory
equipment.
Facilities should be adequately wired according to local building codes.
Power Outages If the IVIS Imaging System experiences a loss of supply power, turn off the
power switch for all components and do not restart the system until reliable
power has been restored. For more details on how to restart the system, see
Startup Procedure, page 36.
Overloading
WARNING
!
WARNING! Do not overload wall outlets, extension cords, or integral
convenience receptacles as this can result in a risk of fire or electric shock.
See Electrical Power Requirements, page 21 for requirements.
2.8 Cleaning or Moving the System Components
Cleaning/Liquid Entry
VOLTAGE
VOLTAGE! Do not use liquid or aerosol cleaners and never spill liquid of any
kind on any of the IVIS®Spectrum components. Sprays and liquids that come
into contact with the IVIS System may result in damage to the system or
electrocution. For more details on proper care of the system, see Cleaning the
IVIS Spectrum, page 51.
Moving the IVIS®Spectrum The IVIS®Spectrum is a sensitive, scientific instrument and should be moved
with care. Pay particular attention when rolling the instrument on its casters to
avoid toppling the equipment.
The total height of the instrument is 83 inches (211cm). If necessary, the plastic
dome can be removed for easier passage through doorways. For more details on
removing the dome, see page 55.
If you have any questions about moving the instrument, contact IVIS Imaging
Systems Technology Support.
2.9 Other Equipment, Chemicals, or Supplies
Use of any equipment other than that recommended by this manual has not been
evaluated for safety and, therefore, is the sole responsibility of the user.
No chemicals are required for the operation of the IVIS Spectrum. Other user-
supplied chemicals or materials may be required as part of your specific
biological testing procedures.

2. Important Safety Instructions
10
2.10 Servicing
Refer all servicing to IVIS Imaging Systems Technology Support. If the product
is damaged and requires service, unplug the product from the outlet and contact
technical support. Servicing by anyone other than those authorized by Caliper
voids any warranty covering this product.

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IVIS® Spectrum Hardware Manual
3 Warnings
Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Burn Hazard & Eye Safety . . . . . . . . . . . . . . . . . . . . . . . . 12
Mechanical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Chemical & Biological Safety . . . . . . . . . . . . . . . . . . . . . . 15
Panels, Covers & Modules . . . . . . . . . . . . . . . . . . . . . . . . 15
3.1 Electrical Safety
WARNING
!
WARNING! DO NOT attempt to service the IVIS®Spectrum yourself. Contact
IVIS Imaging Systems Technology Support for electrical service needs.
Although there are no voltages in excess of 45V inside the imaging chamber,
local line voltages can be present inside the electronics cabinets.
CAUTION
!
CAUTION! If cleaning is necessary, wipe the exterior surfaces of the
IVIS Spectrum with a soft, damp cloth only. Do not allow fluids of any
kind to enter the system interior under any circumstances. For more
details on cleaning the system, see page 51.

3. Warnings
12
3.2 Burn Hazard & Eye Safety
Laser
The IVIS Spectrum Imaging Instrument contains an embedded Class 3R Laser
module: 532 nm (visible green) laser source, 5 mW maximum continuous
(CW). The instrument itself is classified as a Class 1 device because the user is
safely protected from the laser beam. The potential hazard of exposure to
service personnel is clearly indicated with a warning label per IEC 60825 and
CDRH requirements.
CAUTION
!
CAUTION! The use of controls or adjustments or performance of
procedures other than those specified herein may result in hazardous
radiation exposure.
NEVER remove back, side, front or internal panels of the instrument while the
laser is powered on. Note that laser light can be easily reflected off of shiny
surfaces. Remove all watches and jewelry before placing hands in the imaging
chamber.
Complies with FDA performance standards for laser products except for
deviations pursuant to Laser Notice No. 50, dated 6/24/2007.
Complies with IEC/EN 60825-1:2007.
Light Source Module The IVIS Spectrum is equipped with a fluorescence light source module and
connecting fiber optic cables that are capable of producing intense light that
could be damaging to the eyes. Additionally, the system is equipped with a
Class 3R laser, which could be an eye hazard.
3.3 Mechanical Safety
The IVIS Spectrum has many internal motorized components that can move
only when the door is closed and locked. Because the imaging platform moves
frequently during routine use, keep loose objects away from the edges of the
platform where they could become jammed.
Note: The actual warning label
mounted on the laser module at
the service access inside the unit
uses non-fluorescing colors and
materials
Figure 3.1 Laser beams for alignment grid

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IVIS® Spectrum Hardware Manual
CAUTION
!
CAUTION! DO NOT defeat any of the safety interlocks. Do not place
anything under the imaging platform.
The imaging platform cannot be moved unless the door is closed and locked. If
the platform moves when the door is unlocked, shut down the system and
contact IVIS Imaging Systems Technology Support.
The IVIS Spectrum contains several delicate optical and mechanical
components. Do not touch these components and avoid sprays or other
contaminates that may damage lenses or other optical-mechanical components.
CAUTION
!
CAUTION! The laser/galvanometer is a very delicate optical
component that is visible when the filter wheel side access panel is
removed (Figure 3.3). DO NOT touch this device or attempt to clean or
adjust it.
Figure 3.2 IVIS Spectrum, imaging chamber door

3. Warnings
14
The laser beam is directed into the imaging chamber through an opening at the
top of the chamber. Never place any object, tool, or fingers into this opening,
otherwise damage to the galvanometer will result.
WARNING
!
WARNING! DO NOT look directly into this opening when the laser is
operating.
Figure 3.3 Filter wheel removable panel
Filter wheel panel