Biotage TurboVap User manual

TurboVap®
User Manual


TurboVap®User Manual | © Biotage 2017
Contents
TurboVap®
User Manual
CONTENTS
System Overview
1 Evaporation Modes
1 Manifolds
2 Racks and Tube Sizes
2 Water Bath
2 Gas Vortex Shearing Action
2 Ventilation
3 Sleep Mode and Automatic Wake-Up
3 Audible Alarm and Lighting
Determine Optimal Evaporation Conditions
4 Determine the Appropriate Water Bath Temperature
4 Determine the Appropriate Gas Flow
5 Determine the Appropriate Evaporation Mode
Define System Settings
6 Sleep Mode and Automatic Wake-Up
7 Calibrate the Water Bath Temperature
Prepare the system
8 Change the Manifold
8 Adjust the Water Level
9 Adjust the Nozzles
9 Set the Water Bath Temperature
 Evaporate
10 Set Up and Start an Evaporation Run
11 Monitor an Evaporation Run
11 Edit an Evaporation Run in Progress
11 End of an Evaporation Run
11 Pause or Abort an Evaporation Run
11 Unload the Samples
 Define Methods
12 Edit or Rename a Method
12 Evaporation Parameters
 Maintenance
13 Clean the Exterior of the System
13 Clean the Racks and Manifolds
13 Clean the Water Bath
14 Replace a Multi Rack Height Adjustment Handle
14 Replace a Multi Rack Vial Spring
14 Replace a Nozzle
14 Replace an End-Point Sensor
14 Replace the Fuse
15 Long Term Storage
 Troubleshooting
16 End-Point
16 Evaporation
16 Gas Supply
16 Sleep Mode and Automatic Wake-Up
16 Water Bath
 General Information
17 Accessories and Spare Parts
17 Manufacturer
17 Contact Us


System Overview
System Overview
TurboVap®is a solvent evaporation system that can easily be
modified between three different system configurations:
» TurboVap LV can process up to 48 samples
in many types and sizes of tubes including
centrifuge tubes and micro-centrifuge tubes.
» TurboVap II can process up to 6 samples in
50 or 200 mL evaporation tubes using a rack
with or without end-point detection.
» TurboVap EH can process up to two Extrahera sample/
collection racks with 24 samples (in each rack) in
12 x 75 mm, 16 x 75 mm, or 18 x 75 mm tubes.
Evaporation Modes
TurboVap enables you to:
» Evaporate until stopped manually.
» Evaporate for a set period of time.
» Evaporate until the end-point sensor detects a liquid
level of 0.2 or 0.7 mL depending on the glassware.
Each sample tube is controlled individually.
» Evaporate for a set period of time after the end-point sensor
detects a liquid level of 0.2 or 0.7 mL depending on the
glassware. Each sample tube is controlled individually.
» Evaporate using a step or ramp gradient of gas flow and
time settings with a maximum of three segments.
» Evaporate using a combination of a
gradient and end-point detection.
End-Point Detection
The TurboVap II rack with end-point sensors (see page 2)
enables the system to automatically stop the evaporation in
a rack position when the liquid level in the tube passes below
the sensor. The final volume of the evaporation depends on
the stem size of the tube. Evaporation tubes with a 0.5 mL stem
concentrate to 0.2 mL, and tubes with a 1.0 mL stem concentrate
to 0.7 mL. They all have a ±5% calibrated mark on the tube for
you to reconstitute to 0.5 or 1.0 mL; see Figure 1.
The end-point sensor tracks any slow changes in the optical
density of the sample to assure that darkening or brightening
of samples does not stop the evaporation prematurely. Once
the sample solution passes below the end-point sensor, the
system stops the evaporation in that rack position or continues
to evaporate for the additional time. The racks with end-point
sensors can be used at bath temperatures up to 60°C.
Figure 1.200 mL evaporation tube with a 1.0 mL stem.
Manifolds
The manifold is selected according to the rack you want to use
(see page 2). There are three manifolds to choose from:
» TurboVap LV manifold with 48 gas nozzles;
see Figure 2. This manifold is used with the
TurboVap LV multi racks. When using the rack with
24 positions, half of the nozzles must be plugged.
» TurboVap II manifold with 6 gas nozzles; see
the left manifold in Figure 3. This manifold
is used with the TurboVap II racks.
» TurboVap EH manifold with 2 x 24 gas nozzles; see the
right manifold in Figure 3. This manifold is used with
the TurboVap EH sample/collection rack holder.
In the software, it is possible to activate or deactivate each
nozzle row (TurboVap LV or EH) or nozzle (TurboVap II) separately.
If not using all rack positions in an activated nozzle row, single
nozzles can be plugged.
Note that the gas flow displayed in the software is always the
gas flow per nozzle without any nozzles plugged. To have the
correct gas flow shown when using the 24-position multi rack
or the TurboVap EH sample/collection rack holder with only
one rack, i.e. when 24 nozzles are plugged on the manifold
(see Figure 2 on how to plug the TurboVap LV manifold), the
24-position or Extrahera 1 x 24 manifold option must be
selected in the software's settings view (see page 6).
Figure 2.TurboVap LV manifold with 48 nozzles. In the picture to the right,
24 nozzles are plugged for usage with the multi rack with 24 positions.
Figure 3.TurboVap II manifold with 6 nozzles (left) and TurboVap EH
manifold with 2 x 24 nozzles (right).

TurboVap®User Manual | © Biotage 
System Overview
Racks and Tube Sizes
The following TurboVap racks are available:
» TurboVap LV multi racks:
» 48 positions for 10–16 mm (OD),
<45 mm (length) mini vials
» 48 positions for 10–20 mm (OD), 75–165 mm
(length) tubes (see left rack in Figure 4)
» 24 positions for 12–30 mm (OD), 75–165 mm
(length) tubes (see right rack in Figure 4)
» TurboVap II rack with or without end-point sensors;
see Figure 5. The rack has 6 positions for 50 or 200 mL
evaporation tubes with 0.5 or 1.0 mL end volume (C128506,
C128507, C128508, C128511, and C128512).
» TurboVap EH sample/collection rack holder with 2 positions
for Extrahera sample/collection racks with 24 positions for
12 x 75 mm, 16 x 75 mm, or 18 x 75 mm tubes; see Figure 6.
Figure 4. TurboVap LV multi racks with 48 positions (left) and
24 positions (right).
Figure 5
. TurboVap II rack with (left) and without (right) end-point sensors.
Figure 6. TurboVap EH sample/collection rack holder (left) can hold
two Extrahera sample/collection racks with 12 x 75 mm tubes (middle),
16 x 75 mm tubes, or 18 x 75 mm tubes (right).
Water Bath
The water bath operates over a temperature range from ambient
to 90°C. Racks with end-point sensors can only be used at bath
temperatures up to 60°C. The evaporation is automatically
paused (the gas is turned off) when the lid is opened and
resumed when the lid is closed.
The water bath must be kept clean and only be filled with
deionized water. The bath can easily be emptied by connecting
the drain tube supplied with the system to the drain port on the
front right corner.
Gas Vortex Shearing Action
The system uses a gas vortex shearing action to maintain high
evaporation rates under mild thermal conditions regardless of
the sample height in the tube.
A helical flow (see Figure 7) is created by the stream of gas
directed into each sample tube through the nozzles on the
manifold. The helical flow sets up a vortex shearing action that
provides for sample homogeneity and continuous rinsing of the
tube wall. The vapor-laden gas exits via an unobstructed path up
the center portion of the tubes and is removed by an exhaust fan.
Figure 7. Gas vortex shearing action.
Ventilation
Solvent vapors are removed by an exhaust fan and routed to
the exhaust port at the rear of the system. While a laboratory
fume hood is an ideal location for installation of the system,
any location is acceptable if the system is adequately vented
through a proper ventilation system. For more information, see
the TurboVap®Installation and Safety document.


System Overview
Sleep Mode and Automatic Wake-Up
The system can be put in sleep mode either manually or by
scheduling a sleep time. It will also enter sleep mode if the lid is
left open for more than 90 minutes. When entering sleep mode,
the system turns off the heater and the water bath light (if on),
and activates the screen saver. When the water bath temperature
drops below 35°C, the exhaust fan speed is lowered.
The sleep mode feature will not interrupt an ongoing run. If the
system is used at the scheduled sleep time, the system will
wait 30 minutes after the last user interaction before entering
sleep mode.
The system will wake up either at a scheduled wake-up time
or when a user deactivates the sleep mode. The system turns
on the heater and the water bath light (if enabled) and starts
heating the water bath to the set starting bath temperature
(when started at scheduled wake-up time) or the last used water
bath temperature (when started manually).
Audible Alarm and Lighting
To provide better visibility for the user, there is a light strip
located inside the water bath. The light can be turned on or off
in the software.
When the specified evaporation time is reached or the end-point
level (+ any additional time) is reached for all tubes, the system
turns off the gas flow, changes the color of the light strip to
green (see Figure 8), and sounds an alarm. The alarm continues
to beep every 30 seconds until the Evaporation Finished dialog
is closed or the lid is opened.
An audible alarm will also sound (but only once) when the
target bath temperature is reached, the end-point level (+ any
additional time) is reached for a single tube, or an error occurs.
The alarm volume can be set to Low, Medium, High, or OFF.
Figure 8.When a run is completed, the light in the water bath changes to
green.

TurboVap®User Manual | © Biotage 
» As the sample volume decreases during a
concentration, increase the gas flow manually or by
using a gradient (see method mode on the next page).
This will reduce the overall evaporation time.
» Plugged nozzles will increase the gas flow. This is not
reflected by the software. The gas flow displayed in
the software is always the flow per nozzle without any
nozzles plugged. To have the correct gas flow shown
when using the 24-position multi rack or the TurboVap EH
sample/collection rack holder with only one rack, i.e. when
24 nozzles are plugged on the manifold (see Figure 2 on
page 1 on how to plug the TurboVap LV manifold), the
24-position or Extrahera 1 x 24 manifold option must be
selected in the software's settings view (see page 6).
Convert Pressure to Gas Flow
If you want to use a method from a previous system model,
the table below shows how to convert the pressure on the old
system to the gas flow on the new system. We recommend that
these gas flow values are only considered as guidelines and
that the gas flow is re-optimized for the new system according
to the bullet list above.
Pressure Gas Flow
PSI Bar LV (L/min) II (L/min)
20.1 0.7 1.3
50.3 1.2 1.8
70.5 1.5 2.2
10 0.7 1.8 2.8
12 0.8 2.0 3.2
14 1.0 2.2 3.5
16 1.1 2.5 3.8
18 1.2 2.7 4.2
20 1.4 2.9 4.5
Table 1.The pressure set on an old system converted to the gas flow on
the new system.
Determine Optimal Evaporation Conditions
Several variables, including sample tube size, sample volume,
and solvent boiling point, influence the settings required for an
evaporation.
Determine the settings that will best concentrate your samples
by performing a test run using a tube with the approximate
amount of solvent you want to evaporate. Start with the gas
flow set to the lowest setting and then slowly increase the gas
flow until a swirling action without splashing is observed inside
the tube. If you will be using any of the evaporation modes that
require a time setting, check the elapsed time in the software's
status field when the sample has been evaporated to dryness or
to the desired liquid level.
Determine the Appropriate
Water Bath Temperature
The water bath eliminates hot spots and improves sample
recovery for more volatile compounds, and the system’s high
evaporation rates eliminate the need for a high temperature
water bath.
The system is not designed to cool, but the water bath
temperature can be decreased from ambient by as much as
15°C by using the evaporation as a cooling source. Cooling
ability varies with the evaporation rate.
Consider the following when selecting the water bath
temperature:
» Higher water bath temperature speeds up the
evaporation and can increase recoveries. However,
highly volatile samples can be lost if allowed to sit for
extended periods of time in a warm water bath.
» Boiling the solvent may incorrectly trigger the end-point
sensors and can risk loss of sample. Select a water bath
temperature below the boiling point of your solvent.
» Racks with end-point sensors can only be
used at bath temperatures up to 60°C.
Determine the Appropriate Gas Flow
Consider the following when selecting the operating gas flow:
» Higher gas flow causes faster evaporation rates.
» Excessively high gas flow can cause loss of sample
and cross-contamination due to splashing.
» For the best results, always use the highest gas
flow possible without causing splashing.
Determine Optimal Evaporation Conditions


Determine the Appropriate
Evaporation Mode
There are five evaporation modes available (see Figure 9):
» Manual: Evaporate until the user presses the Stop button.
» Time: Evaporate for a set period of time.
» End Point: Evaporate until the end-point sensors
detect a liquid level of 0.2 or 0.7 mL depending on the
glassware. Each sample tube is controlled individually.
» End Point + Time: Evaporate for a set period of time after the
sensor detects a liquid level of 0.2 or 0.7 mL depending on
the glassware. Each sample tube is controlled individually.
» Method: There are several options:
» Evaporate using a step or ramp gradient of gas flow
and time settings with a maximum of three segments.
» Evaporate using end-point detection; see
End-Point and End-Point + Time above.
» Evaporate using a combination of a
gradient and end-point detection.
» Evaporate for a set period of time.
Notes
» The rack with end-point sensors cannot be used if:
» The water bath temperature is above 60°C.
» Sample coats the glass or darkens too rapidly.
» The desired end-point volume is
greater than 0.2 or 0.7 mL.
» The two end-point detection modes are only
enabled when a TurboVap II end-point rack has
been connected to the system's AUX port.
Figure 9
Evaporate Settings System Ready
12:44 Wed 23 Nov 2016
Manual
Time
Mode
Edit
Nozzle Setup
Start
Stop
Gas Flow
L/min
0.0
1.2 L/min
Bath Temperature
75 °C
°C
34
0 min
Time Elapsed
Time
End-Point
Manual
End-Point + Time
Method
Mode
.The different evaporation modes that can be selected in the
software.
Determine Optimal Evaporation Conditions


TurboVap®User Manual | © Biotage 2017
Define System Settings
Define System Settings
Enter the software's settings view (see Figure 10) by pressing
Settings in the menu bar. The following system settings are
available:
» Sound Level: The system will sound when the target
bath temperature is reached, the end-point level
(+ any additional time) is reached for a single tube,
a run is completed, or an error occurs. The sound
level can be set to Low, Medium, High, or OFF.
» Brightness: The brightness of the touch screen.
» Manifold Setup: The installed manifold. For more
information, see "Manifolds" on page 1. For instructions
on how to change the installed manifold, see page 8.
» Sleep Mode: See "Sleep Mode and Automatic Wake-Up" below.
» Temp Unit: The temperature unit in use by the system,
degrees Celsius or Fahrenheit. Toggle by pressing Change.
» Date: The current date.
» Time: The current time (24 hour time).
» Bath Light: The bath light can be turned ON or OFF.
By pressing the More button in the settings view, the following
information and features will be available: 1) software versions,
instrument serial number, and end-point board serial number
(if connected), 2) trademarks and copyright information,
3) button for factory reset, 4) button for accessing the
calibration of the water bath temperature, and 5) button for
accessing the wizard for calibrating the touch screen.
Figure 10
Evaporate Settings System Ready
16:48 Wed 23 Nov 2016
Time & Date
Time 16 48
:
Date 2016Nov23
Manifold Setup
48 Positions
Sound Level
Medium
Sleep Mode
Edit
Temp Unit
°C
Brightness
- +
Bath Light
OFF ON
More »
Change
.The Settings view.
Sleep Mode and Automatic Wake-Up
The system can be put in sleep mode either manually by
pressing the orange button in the settings view (see Figure 10)
or automatically by scheduling a sleep time in the sleep mode
view (see Figure 11). The system will also enter sleep mode if the
lid is left open for more than 90 minutes.
When entering sleep mode, the system turns off the heater and
the water bath light (if on), and activates the screen saver. When
the water bath temperature drops below 35°C, the exhaust fan
speed is lowered.
The sleep mode feature will not interrupt an ongoing run. If the
system is used at the scheduled sleep time, the system will
wait 30 minutes after the last user interaction before entering
sleep mode.
The system will wake up either at a scheduled wake-up time
or when a user deactivates the screen saver (by touching the
screen) and then presses Wake Up. The system turns on the
heater and the water bath light (if enabled) and starts heating
the water bath to the set starting bath temperature (when
started at scheduled wake-up time) or the last used water bath
temperature (when started manually).
Sleep Mode and Automatic Wake-Up Parameters
The following parameters are available in the sleep mode view:
» Starting Bath Temp: The target water bath temperature when
starting up from sleep mode at the scheduled wake-up time.
» Wake-Up Time: The time when the system will turn on
the heater and the water bath light (if enabled) and
start heating the water bath to the set starting bath
temperature. The system will start in manual mode.
» Sleep Time: The time when the system will
turn off the heater and the water bath light
(if on), and activate the screen saver.
» Weekdays: The weekdays that the sleep
mode settings will be used.
Figure 11
Evaporate Settings System Ready
Time Elapsed:
OK
Weekdays
55 °C
Starting Bath Temp
7 8 9
0 Clear
4 5 6
1 2 3
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
07 30
:
OFF ON
18 10
:
OFF ON
Sleep Mode
Wake-Up Time Sleep Time
Save
Cancel
.The Sleep Mode view.


Define System Settings
Calibrate the Water Bath Temperature
The water bath temperature can be adjusted after calibrating at
two temperatures that encompass (above and below) the range
of temperatures used in your applications.
1. Set the evaporation mode to Manual or Time.
2. Set the first water bath temperature by pressing the button
in the Bath Temp field.
3. Lower a temperature probe into the water bath and close
the lid.
4. Allow the water bath temperature to stabilize and then take
note of the measured temperature.
5. Repeat steps 2 through 4 with the second water bath
temperature.
6. When you are done, press More in the settings view and
then Calibrate Temp.
7. Enter the values of set and measured temperatures into the
Temp 1 and Temp 2 fields; see Figure 12.
8. Press Calculate to calculate new temperature coefficients to
be used by the system.
9. Press Save.
10. Verify the calibration by repeating steps 2 through 5.
Figure 12
Evaporate Settings System Ready
Time Elapsed:
Current Cal
New Cal
789
0 . Clear
456
123
Calibrate Bath Temp Sensor
42 °C
39.2 °C
65 °C
10.0
0.0
2.1
0.0
65.4 °C
Temp 1
Set Set
C0
C0
C1
C1
Measured Measured
Temp 2
Calculate
Save
Cancel
.The Calibrate Temperature view.


TurboVap®User Manual | © Biotage 2017
Prepare the System
1. If a rack is present in the water bath, carefully lift it out of the
bath, clean it using a non-alkaline detergent, and let it dry.
2. Lower the water level to the minimum level marked on the
water bath:
a. To avoid the risk of burning yourself with the water or on
the drain connector, lower the water bath temperature to
below 55°C.
b. When the water bath temperature is below 55°C, insert
the drain tube supplied with the system into a waste
container that can take at least 12 liters.
c. Push in the drain tube connector into the DRAIN port on
the front right corner of the system; see Figure 14A.
d. When the water level is at the minimum level marked on
the water bath (see Figure 14B), disconnect the drain tube
by pressing on the top of the DRAIN port (see Figure 15A).
e. Empty the waste container.
3. Gently lower the desired rack, with empty tubes in all
positions except one, into the water bath.
4. Place a funnel (not supplied) in the empty rack position and
add deionized water until the water’s surface is as high as
the initial liquid level will be in the tubes but not higher than
just below the blue water bath top cover; see Figure 15B.
5. Add water treatment according to the supplier's
recommendations. We recommend Aqua Stabil
(P/N 415656SP).
Figure 14
AB
MIN
.A: Insert the drain tube into a waste container before pushing
in the drain tube connector into the DRAIN port. B: The minimum level on
the water bath.
Figure 15
AB
. A: Disconnecting the drain tube by pressing on the top of the
DRAIN port. B: Add deionized water until the water’s surface is as high as
the initial liquid level will be in the tubes but not higher than just below the
blue water bath top cover.
Prepare the System
Change the Manifold
Warning
» The nozzles have sharp edges and may have come into contact
with harmful sample residues. Avoid contact.
There are three different manifolds available for the system.
The manifold is selected based on the rack you want to use.
For more information, see "Manifolds" on page 1.
1. With the lid closed, remove the four screws on the top of the
lid (see Figure 13A).
2. Open the lid and remove the cover for the gas connections
on the manifold.
Figure 13
AB
.A: The four screws on the top of the lid holds the manifold in
position. B: The gas connections on the manifold.
3. Disconnect the six gas tubes from the manifold. A tube is
released by pushing the tube in, pushing the release collar
into the fitting, and then pulling out the tube. See Figure 13B.
4. Clean the manifold using a non-alkaline detergent and let it
dry. Store the manifold in a clean and dust free environment.
5. Connect the gas tubes to the desired manifold. The gas
tubes and nozzle rows or nozzles are numbered from 1 to 6.
6. Place the cover over the gas connections.
7. Close the lid.
8. Fasten the manifold to the lid using the four screws.
Adjust the Water Level
Warning
» Always ensure that the drain tube is inserted into a waste
container of a suitable size before connecting the drain tube to
the system’s drain port.
» The temperature of the water bath and the drain connector can
be up to 90°C. Avoid contact.
The water level must be adjusted for the selected rack and
sample tube type. To avoid flooding the water bath, we
recommend the following procedure.


Prepare the System
Adjust the Nozzles
Warning
» The nozzles have sharp edges and may have come into contact
with harmful sample residues. Avoid contact.
Note: The nozzles on the TurboVap II manifold have fixed
positions and cannot be adjusted.
Note: Incorrectly adjusted nozzles will not generate a vortex
shearing action and result in poor evaporation speeds, and
may also result in broken tubes and samples leaking into the
water bath.
The nozzles must be adjusted for the selected rack and tube type.
To avoid broken tubes, we recommend the following procedure.
1. Ensure that the water level is correct; see instructions above.
2. If using the TurboVap LV multi rack with 24 positions or the
TurboVap EH sample/collection rack holder with only one
rack, plug the nozzles that are not in use. See Figure 2 on
page 1 on how to plug the TurboVap LV manifold.
3. Ensure that the desired rack is available in the water bath
with one empty tube of the correct type.
4. Adjust the nozzle row with the empty tube so that the
nozzles are as far to the left as possible.
5. Close the lid and adjust the nozzle row with the empty tube
so that the nozzles are as far to the right as possible without
the nozzle touching the side of the tube.
6. Open the lid and adjust the other nozzle rows into the
same position using the engraved adjustment lines on the
manifold; see Figure 16.
Figure 16.The engraved adjustment lines on the manifold.
Set the Water Bath Temperature
Warning
» The temperature of the water bath and the drain connector can
be up to 90°C. Avoid contact.
» Do not operate the system without water in the water bath.
1. If applicable, close the lid and turn on the system. The mains
switch is located on the right side of the system.
Note: The system should always be on whenever it contains
samples so that the fan can evacuate solvent vapors.
2. Set the water bath temperature by pressing the button in the
Bath Temp field. For more information, see "Determine the
Appropriate Water Bath Temperature" on page 4. Note that
this button is not enabled in method mode; see page 12.
3. When the target water bath temperature has been reached,
the user will be notified by a dialog on the touch screen (see
Figure 18) and an audible alarm.
Figure 17
Evaporate Settings System Ready
10:30 Wed 23 Nov 2016
Manual
Mode
Edit
Nozzle Setup
Start
Stop
Gas Flow
L/min
0.0
1.2 L/min
Bath Temp
75 °C
°C
34
Status
0:00
Elapsed (min:sec)
.When the heater is on, a red, blinking dot is displayed in front
of the current water bath temperature.
Figure 18
Manual
Time
Mode
Edit
Nozzle Setup
0 min
Time Elapsed
Start
Stop
Gas Flow
L/min
0.0
1.2 L/min
Bath Temperature
55 °C
°C
75
Evaporate System Ready
10:30 Wed 23 Nov 2016
Settings
Target bath temperature
has been reached.
OK
.The system alerts the user when the target bath temperature
has been reached.


TurboVap®User Manual | © Biotage 2017
Evaporate
Note: If the system is not used for an extended period of time,
we recommend that it is put in sleep mode. See "Sleep Mode
and Automatic Wake-Up" on page 6.
Note: To prevent condensation within the system, the lid should
always be left open when the system is turned off and the water
bath contains water.
Set Up and Start an Evaporation Run
1. Prepare the system as described on page 8 to page 9.
2. Check that the incoming gas supply is turned on and has
sufficient reserve for the run.
3. If using a rack with end-point sensors, gently lower the rack
(empty) into the water bath and connect the sensor connection
box to the AUX port on the right side of the system.
4. Select Evaporate from the menu bar.
5. Set up the evaporation parameters; see "Evaporation
Parameters" below.
6. When the target water bath temperature has been reached
(see Figure 18 on page 9), either load the sample tubes
into the rack when positioned inside the water bath or gently
lower the rack with the sample tubes into the water bath. The
latter is not possible if using a rack with end-point sensors.
7. Close the lid and press Start.
Evaporation Parameters
The following evaporation parameters are available:
» Mode: The selected evaporation mode; see "Determine
the Appropriate Evaporation Mode" on page 5.
Note: The two end-point detection modes are only enabled
when a TurboVap II end-point rack has been connected to
the AUX port. After connection, it will take a few seconds
before the two modes are enabled.
» Method: The selected evaporation method. Only available
in method mode. To modify a method, see page 12.
» Time: The evaporation time (when in time mode) or
the amount of time the evaporation continues in a
sample tube after its liquid level passes below the
sensor (when in the end-point + time mode).
» Gas Flow: The internal gas flow directed at each
of the sample tubes. For more information, see
"Determine the Appropriate Gas Flow" on page 4.
To change for a method, see page 12.
» Bath Temp: The target water bath temperature. For
more information, see "Determine the Appropriate
Water Bath Temperature" on page 4. To change
for a method, see page 12.
» Nozzle Setup: Defines whether a nozzle row (when
using racks with 24 or 48 positions) or a nozzle
(when using racks with 6 positions) is enabled (ON)
or not (OFF). Ensure that the nozzles for all positions
that will contain sample tubes are enabled.
Note: If not using all rack positions in an activated nozzle
row, single nozzles can be plugged. Note that the gas flow
displayed in the software is always the gas flow per nozzle
without any nozzles plugged. To have the correct gas
flow shown when using the 24-position multi rack or the
TurboVap EH sample/collection rack holder with only one rack,
i.e. when 24 nozzles are plugged on the manifold (see Figure 2
on page 1 on how to plug the TurboVap LV manifold),
the 24-position or Extrahera 1 x 24 manifold option must be
selected in the software's settings view (see page 6).
Figure 19
Evaporate Settings System Ready
16:21 Wed 23 Nov 2016
Method
Method 3
Method
65 °C1.2 L/min
Mode
Gas Flow
Start
Stop
L/min
0.0 °C65
Edit
Nozzle Setup
Bath Temp
Status
0:00
Elapsed (min:sec)
54:00
Remaining (min:sec)
.The Evaporate view when method mode is selected.
Figure 20
Evaporate Settings Evaporating
15:51 Wed 23 Nov 2016
Edit
Evaporate
7:35 min Done
6:53 min
Done
4:01 min
55 °CPause
Stop
End-Point + Time
Mode
Nozzle Setup
°C
55
1.2 L/min
Gas Flow
L/min
1.2
15 min
+Time
Status
Bath Temp
45:55
Elapsed (min:sec)
.The run view when end-point + time mode is selected.
Evaporate


Evaporate
Monitor an Evaporation Run
During the evaporation run, the current and set values for the
water bath temperature, gas flow, and time can be monitored on
the touch screen; see Figure 20. If the difference between the
actual and the set water bath temperature is more than ±2°C,
the user will be notified by a dialog on the touch screen and an
audible alarm. If using end-point sensors, it is also possible to
see when a specific end-point sensor is triggered and when the
tube is completed.
If running in method mode, the evaporation parameters for the
selected method can be displayed by pressing the button in the
Method field.
Edit an Evaporation Run in Progress
During the run, it is in most cases possible to change the gas
flow and the water bath temperature, and enable or disable
nozzle rows or nozzles. When running in method mode, it is not
possible to change the gas flow or bath temperature, and when
using a rack with end-point sensors it is not possible to enable
or disable nozzles.
End of an Evaporation Run
If the evaporation run is not ended automatically (when manual
mode is selected), end the run by pressing Stop.
When the specified evaporation time is reached or the end-point
level (+ any additional time) is reached for all tubes, the system
turns off the gas flow, changes the light inside the water bath
to green (see Figure 8 on page 3), and sounds an alarm. The
alarm continues to beep every 30 seconds until the Evaporation
Finished dialog (see Figure 21) is closed or the lid is opened.
Figure 21
Evaporate
00:00
Time Remaining (min:sec)
Finalizing
12:44 Wed 23 Nov 2016
Time
Settings
54 min
Time
31 min
Time Elapsed
Pause
Stop
1.2 L/min 75 °CEdit
Mode
Gas Flow Bath Temperature Nozzle Setup
54 min
L/min
1.2 °C75
Status
54:00 min
Total Time Elapsed
Evaporation Finished
OK
.The Evaporation Finished dialog is displayed when the specified
evaporation time is reached or the end-point level (+ any additional time)
is reached for all tubes.
An audible alarm will also sound (but only once) each time the
end-point level (+ any additional time) is reached for a single tube.
Note: The alarm and water bath light can be turned off in the
settings view;
see page 6 .
Pause or Abort an Evaporation Run
To pause the run, either press Pause or lift the lid. The gas flow
is turned off and the timer is paused. The evaporation can be
resumed by pressing Resume or, if the pause was triggered by
the lid being opened, by closing the lid.
To abort the run before the specified evaporation time has been
reached or the end-point level has been reached for all tubes,
press Stop.
To turn off the gas flow for a single nozzle row or nozzle, disable
it by pressing the Edit button in the Nozzle Setup field. Note that
this is not possible when using a rack with end-point sensors.
Unload the Samples
Open the lid and remove the sample tube(s) or carefully lift the
whole rack out of the water bath.
If using end-point sensors, reconstitute to the desired volume
(0.5 or 1.0 mL) and, if necessary, use this volume to rinse the
angled part of the tube and end-point stem several times to
remove any sample residues on the tube wall.
Note: Prompt removal of sample tubes is important since highly
volatile compounds can be lost if allowed to sit for an extended
period of time.
Figure 22.A rack left shortly above the water bath to drain.


TurboVap®User Manual | © Biotage 2017
Define Methods
Define Methods
It is possible to save up to ten different evaporation methods.
The methods can be set up to:
» Evaporate using a step or ramp gradient of gas flow and
time settings with a maximum of three segments.
» Evaporate using end-point detection; see the End-Point
and End-Point + Time modes on page 5.
» Evaporate using a combination of a
gradient and end-point detection.
» Evaporate for a set period of time.
Edit or Rename a Method
If you want to edit or rename a method before your run it,
proceed as follows:
1. Select Evaporate from the menu bar.
2. Press the button in the Mode field and select Method in the
appearing dialog.
3. Press the button in the Method field. The Method dialog
opens; see Figure 23.
4. Select the method you want to modify and then press Edit or
Rename.
5. In the appearing dialog, edit the desired evaporation
parameters (see"Evaporation Parameters" below) or enter
the desired name.
6. When done, press Save.
7. In the method view, press OK to return to the Evaporate view.
Figure 23
Evaporate Settings System Ready
Ramp gradient
Method 2
Method 6
Method 3
Method 7
Method 4
Method 8
Method 5
Method 9
Method 10
Method Summary
Bath Temp: 50 °C
1.2 L/min
Ramp
2.2 L/min
2.8 L/min
5 min
15 min
15 min
ON
10 min
Step 1:
Gradient:
Step 2:
Step 3:
End-Point:
Rename
Edit OK
Method
.The Method view where you select the method you want to run,
or modify a method and then run it.
Evaporation Parameters
The following evaporation parameters are available in the edit
method view (see Figure 24):
» Gradient Type: A step or ramp gradient with up to three
segments with different gas flows and lengths (time).
If you only want to evaporate for a set period of time or
using end-point detection, turn OFF the gradient feature.
» Step 1–3: Each gradient segment consists of a setting
for the gas flow directed at each of the sample tubes and
the duration time. If the gradient is turned OFF, enter
the gas flow and, if not using end-point detection, the
evaporation time in the Step 1 field. For more information,
see "Determine the Appropriate Gas Flow" on page 4.
» End-Point Detection: If this option is enabled, the
evaporation is ended in a rack position when the end-point
sensor detects a liquid level of 0.2 or 0.7 mL depending
on the tube’s stem size. If a time is also entered, the
evaporation continues for the amount of time that is
specified after the liquid level is detected. The end-point
detection option can be used in combination with a gradient.
» Bath Temp: The target water bath temperature. For
more information, see "Determine the Appropriate
Water Bath Temperature" on page 4.
Figure 24
Evaporate Settings System Ready
789
0 . Clear
456
123
1.2 L/min
5 min
2.2 L/min
15 min
2.8 L/min
15 min
Gradient Type
50 °C
Save
Cancel
Edit Method
Ramp gradient
Step 1 Step 2 Step 3
Bath Temp
Step Ramp OFF
OFF ON
0 min
+ Time:
End-Point Detection
.The Edit Method view.


Maintenance
Maintenance
Warning
» Always turn off the system, unplug the power cord, and let the
water bath cool down before performing maintenance.
» There are no user serviceable parts inside the system. Covers
and safety shields may only be removed by an authorized
Biotage service engineer. Potential electrical hazard exists due
to high voltage circuits inside the system.
» The power cord should be inspected periodically and replaced if
damaged or altered. Use only a power cord supplied by Biotage.
» Handle chemical and liquid waste according to the Safety Data
Sheets and to local/national guidelines on laboratory safety
procedures.
Clean the Exterior of the System
Clean the exterior of the system as often as required. If the
touch screen has been contaminated by chemicals, it must be
cleaned immediately.
1. Turn off the system and disconnect the power cord.
2. Clean the touch screen and the exterior of the system with a
soft and clean cloth. The cloth can be lightly dampened with
isopropanol or ethanol.
Clean the Racks and Manifolds
Warning
» The nozzles have sharp edges and may have come into contact
with harmful sample residues. Avoid contact.
The racks and manifolds must be cleaned after spillage or
before storage. Clean using a non-alkaline detergent. Store in
a clean and dust free environment.
Clean the Water Bath
Warning
» If spilling sample or solvent into the water bath, immediately
clean the water bath.
Clean the water bath as often as required but at least once a
week.
1. To avoid the risk of burning yourself on the water or the drain
connector, lower the water bath temperature to below 55°C.
2. If a rack is present in the water bath, carefully lift it out of the
bath, clean it using a non-alkaline detergent, and let it dry.
3. When the water bath temperature is below 55°C, turn off the
system and disconnect the power cord.
4. Empty the water bath:
a. Insert the drain tube supplied with the system into a
waste container that can take at least 12 liters.
b. Push in the drain tube connector into the DRAIN port on
the front right corner of the system; see Figure 25A.
c. When the water bath is empty, disconnect the drain tube
by pressing on the top of
the DRAIN port (see Figure 25B).
d. Empty the waste container.
Figure 25
AB
.Connecting (A) and disconnecting (B) the drain tube.
5. Clean the bath walls with an appropriate cleaning solution
and then rinse.
6. Empty the water bath; see step 4.
7. Pour 4.5 liters of deionized water into the water bath; see
Figure 26A. The water level should now be at the minimum
level marked on the water bath.
8. Gently lower the desired rack, with empty tubes in all
positions except one, into the water bath.
9. Place a funnel (not supplied) in the empty rack position and
add deionized water until the water’s surface is as high as
the initial liquid level will be in the tubes but not higher than
just below the blue water bath top cover; see Figure 26B.
10. Add water treatment according to the supplier's
recommendations. We recommend Aqua Stabil
(P/N 415656SP).
Figure 26
AB
4.5 liters
.The water’s surface must be above the minimum level marked
on the water bath but not higher than just below the blue water bath
top c over.


TurboVap®User Manual | © Biotage 2017
Maintenance
Replace an End-Point Sensor
If the software reports a calibration error, there might be air
bubbles around the sensor optics. Dislodge the air bubbles
by moving the tube up and down a couple of times. If this
does not solve the problem, replace the end-point sensor as
described below.
1. Remove the broken end-point sensor by sliding it to the
middle position and then to the side; see Figure 29.
Figure 29.Removing a broken end-point sensor.
2. Unsnap the sensor cable from the five or six clips holding it
in position.
3. Disconnect the sensor cable from the sensor connection box.
4. Install a new end-point sensor (P/N 415229SP). See Figure 30
on how to route the cable.
Figure 30











.The wiring of the end-point sensors.
Replace the Fuse
Warning
» Use only exact replacement fuses supplied by Biotage. Incorrect
fuses create a potential fire hazard and personal injury.
1. Turn off the system and disconnect the power cord.
2. Using a flat-bladed screwdriver, push and turn the slotted
center of the fuse holder counterclockwise until the holder
pops out; see Figure 31.
3. Clean the new fuse (P/N 415578SP) using a cloth lightly
dampened with ethanol and wipe it dry with a dry cloth.
Note: Do not touch the metal surfaces after the fuse has
been cleaned.
Replace a Multi Rack Height
Adjustment Handle
A multi rack height adjustment handle only has to be replaced
when broken.
1. Remove the two screws holding the broken handle using a
T8 Torx screwdriver.
2. Slide the handle down to the bottom of the rack and remove it.
3. Fasten a new height adjustment handle (P/N 415232SP) to
the height adjustment bar; see Figure 27. Note that this part
comes in pairs; one left and one right handle.
Figure 27.Replacing a height adjustment handle.
Replace a Multi Rack Vial Spring
A multi rack vial spring only has to be replaced when broken.
1. Release the vial spring by squeezing it together at the top
below the rack’s top plate; see Figure 28.
2. Remove the vial spring by lifting it straight up.
3. Insert a new vial spring (P/N 415231SP).
Figure 28.Releasing a vial spring.
Replace a Nozzle
Warning
» The nozzles have sharp edges and may have come into contact
with harmful sample residues. Avoid contact.
A nozzle only has to be replaced when broken or clogged.
Replace the nozzle (P/N 414892SP) and finger tighten, and then
tighten 1/6 turn using a 5 mm hexagon socket wrench.


Maintenance
4. Replace the old fuse.
5. Put the fuse holder back in place by pushing it in and turning
it clockwise until it locks.
If the fuse blows shortly after replacing it, please contact
Biotage®1-Point Support™.
Figure 31.Removing the fuse holder.
Long Term Storage
Warning
» The nozzles have sharp edges and may have come into contact
with harmful sample residues. Avoid contact.
1. Turn off the system and disconnect the power cord.
2. Turn off the gas supply.
3. If a rack is present in the water bath, carefully lift it out of the
bath, clean it using a non-alkaline detergent, and let it dry.
4. Empty and clean the water bath as described in steps 4
through 6 in "Clean the Water Bath" on page 13, and allow
it to dry.
5. Clean the exterior of the system as described on page 13.
6. If necessary, clean the manifold using a non-alkaline
detergent.
7. Store the system in a dust free environment with the lid
closed.


TurboVap®User Manual | © Biotage 2017
Troubleshooting
Troubleshooting
End-Point
» If the end-point modes are disabled in the
software, ensure that an end-point rack has
been connected to the system's AUX port.
» If the software reports a calibration error, there might
be air bubbles around the sensor optics. Dislodge the
air bubbles by moving the tube up and down a couple
of times. If this does not solve the problem, replace
the end-point sensor as described on page 14.
Evaporation
» If the evaporation is uneven between tubes, one or more
nozzles may be leaking and need to be tightened or
replaced. A nozzle should be finger tightened and then
tightened 1/6 turn using a 5 mm hexagon socket wrench.
Check for leakage by applying water around the suspected
area (see image below) and starting a run with the lid
open (press Override in the open lid warning dialog).
» If the evaporation rate is too slow:
» Ensure that the nozzles are adjusted properly;
see "Adjust the Nozzles" on page 9.
» Ensure that the water level is not too low; see
"Adjust the Water Level" on page 8.
» Ensure that the water bath temperature is
not too low; see "Determine the Appropriate
Water Bath Temperature" on page 4.
» Ensure that the gas flow is not too low; see "Determine
the Appropriate Gas Flow" on page 4.
» If the evaporation rate is too fast or splashing occurs:
» Ensure that the water bath temperature is not too high
and causing the solvent to boil; see "Determine the
Appropriate Water Bath Temperature" on page 4.
» Ensure that the gas flow is not too high; see "Determine
the Appropriate Gas Flow" on page 4.
» If there is no gas flow:
» Ensure that the gas supply is turned on and that
there is enough gas available for the run.
» Ensure that the gas inlet tube is not obstructed.
» If there is no evaporation in a row of tubes, ensure
that the nozzles for all rack positions that contain
sample tubes are enabled in the software.
» If there is no or slow evaporation in a single tube:
» When using a system with TurboVap II configuration,
ensure that the nozzles for all rack positions that
contain sample tubes are enabled in the software.
» The nozzle may be blocked. Replace it; see page 14.
» If the water bath temperature and gas flow settings
are disabled in the Evaporate view, the evaporation
mode is set to Method. To change the temperature
and/or gas flow settings for a method, see "Define
Methods" on page 12. Note that it is not possible
to change these settings when running a method.
» If the recovery of sample is low:
» Ensure that the water bath temperature is not too high
and causing the solvent to boil; see "Determine the
Appropriate Water Bath Temperature" on page 4.
» Ensure that the sample is not left too long in the
water bath after the evaporation is completed.
» Ensure that the gas flow is not too high
and causing splashing; see "Determine the
Appropriate Gas Flow" on page 4.
» When using a system with TurboVap II configuration,
check if there is sample residues on the tube wall.
Reconstitute to the desired volume, 0.5 or 1.0 mL,
and use this volume to rinse the angled part of
the tube and end-point stem several times.
Gas Supply
» If there is a leakage at the gas inlet (the N2port):
» Ensure that the gas inlet tube is securely attached.
» Ensure that a gas inlet tube with the correct
outer diameter is used, i.e. 6 mm. Always use
tubing and adapters supplied by Biotage.
Sleep Mode and Automatic Wake-Up
» If the system turns on or off by itself, the sleep
mode feature is activated. To disable or change
the sleep mode settings, see page 6.
Water Bath
» If the water is turbid, clean the water bath and add water
treatment according to the supplier's recommendations.
See "Clean the Water Bath" on page 13.
» If the heating of the water bath is too slow, ensure
that the voltage selector switch (located on the right
side of the system) is in the correct position.


General Information
General Information
Accessories and Spare Parts
Only genuine Biotage accessories must be used in the system.
To order consumables and accessories, see contact information on
the back of this document or visit our website www.biotage.com.
Manifolds and Racks
Part No. Description Qty
415408 TurboVap LV Manifold (48 Nozzles) 1
415489 TurboVap LV Multi Rack
(48 Positions, 10–16 mm Mini Vials)
1
414964 TurboVap LV Multi Rack
(48 Positions, 10–20 mm Tubes)
1
415129 TurboVap LV Multi Rack
(24 Positions, 21–30 mm Tubes)
1
415222 TurboVap II Manifold (6 Nozzles) 1
415535 TurboVap II Rack with End-Point Sensors
(6 Positions, 50 mL Tubes)
1
415100 TurboVap II Rack with End-Point Sensors
(6 Positions, 200 mL Tubes)
1
415494 TurboVap II Rack without End-Point Sensors
(6 Positions, 50/200 mL Tubes)
1
415490 TurboVap EH Manifold (2 x 24 Nozzles) 1
415510 TurboVap EH Sample/Collection Rack Holder
(2 Positions)
1
415491 Sample/Collection Rack
(24 Positions, 12 x 75 mm Tubes)
1
415585 Sample/Collection Rack
(24 Positions, 16 x 75 mm Tubes)
1
415492 Sample/Collection Rack
(24 Positions, 18 x 75 mm Tubes)
1
414892SP Replacement Nozzle for TurboVap 8
C46811 Nozzle Plug for TurboVap 25
415231SP Multi Rack Vial Spring 10
415232SP Multi Rack Height Adjustment Handles (Pair) 2
415229SP Replacement End-Point Sensor for TurboVap II 1
Tubes and Caps
Part No. Description Qty
C48985 10 x 75 mm Test Tube 1000
C44651 12 x 75 mm Test Tube 1000
C40707 13 x 100 mm Test Tube 1000
413282 16 x 75 mm Test Tube 1000
C40708 16 x 100 mm Test Tube 1000
C45273 16 x 125 mm Test Tube 1000
414574 18 x 75 mm Test Tube 304
C40709 20 x 150 mm Test Tube 500
Part No. Description Qty
C47811 10 mL Conical Centrifuge Test Tube 125
C44941 15 mL Conical Centrifuge Test Tube 125
C47816 15 mL Volumetric Test Tube for AutoTrace 12
C44942 Snap Caps for 15 mL Conical Centrifuge Test
Tubes (Polypropylene)
500
C128506 Evaporation Tube TurboVap II, 200 mL, 1 mL
End-Point
12
C128507 Evaporation Tube TurboVap II, 200 mL, 0.5 mL
End-Point
12
C128508 Evaporation Tube TurboVap II, 50 mL, 0.5 mL
End-Point
12
C128511 Evaporation Tube TurboVap II, 50 mL, 1 mL
End-Point
12
C128512 Evaporation Tube TurboVap II, 50 mL,
Centrifuge
12
Misc
Part No. Description Qty
415656SP Aqua Stabil, 100 mL 1
C43067 Venting Hose 50.8 mm (2") ID, 3.8 meters
(12.5 feet) long
1
414742SP Fuse, 10AF/250VAC Fast, 6.3 x 32 mm 1
415814SP Air/N2 Inlet Tubing, 6 mm OD 1
352281SP Pressure Regulator 1
353480SP Supply Connection Set 1
For a complete list, please visit our website www.biotage.com.
Manufacturer
Biotage GB Limited United Kingdom for
Biotage Sweden AB
Contact Us
Biotage Sweden AB
Box 8
SE-751 03 Uppsala
SWEDEN
Visiting address: Vimpelgatan 5
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Website: www.biotage.com
Please contact your local Biotage representative. See contact
information on the back of this document or visit our website
www.biotage.com.

© Biotage 2017
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Part Number: 415390-C
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