Thermo Scientific Sorvall Legend X1 User manual

50120979-3 October 2009
Thermo Fisher Scientific
Sorvall Legend X1
Sorvall Legend X1R
Instruction Manual

© 2009 Thermo Fisher Scientific Inc. All rights reserved.
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Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
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The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-
free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
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Sale shall govern all conflicting information between the two documents.
Release history: 50120979-3 printed in October 2009.
For Research Use Only. Not for use in diagnostic procedures.

WEEE Conformity
This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE)
Directive 2002/96 It is marked by the following symbol:
Thermo Fisher Scientific has entered into agreements with recycling and disposal companies in all EU
Member States for the recycling and disposal of this device. For information on recycling and disposal
companies in Germany and on the products of Thermo Fisher Scientific, which fall under the RoHS
Directive (Restriction of the use of certain hazardous substances in electrical and electronic
equipment), please visit the website www.thermo.com/WEEERoHS

Thermo Scientific Legend X1 / X1R i
T
Preface ....................................................................................................................................... iii
Scope of Supply ....................................................................................................................... iii
Intended Purpose ..................................................................................................................... iii
Accident Prevention .................................................................................................................iv
Precautions ................................................................................................................................iv
Chapter 1 Introduction and Description ............................................................................................... 1-1
Characteristics of the Sorvall Legend X1/X1R .......................................................... 1-2
Technical Data ........................................................................................................ 1-3
Directives, Standards and Guidelines ...................................................................................... 1-4
Functions and Features ........................................................................................................... 1-5
Mains Supply ........................................................................................................... 1-5
Rotor Selection ........................................................................................................ 1-6
Chapter 2 Before use ............................................................................................................................... 2-1
Before Setting up ..................................................................................................... 2-2
Transporting the Centrifuge .................................................................................... 2-2
Location .................................................................................................................. 2-2
Aligning the Centrifuge ........................................................................................... 2-3
Mains Connection ................................................................................................... 2-3
Storage ..................................................................................................................... 2-4
Chapter 3 Control panel .......................................................................................................................... 3-1
Keys ......................................................................................................................... 3-2
Control Panel .......................................................................................................... 3-2
Chapter 4 Operation ................................................................................................................................. 4-1
Switch on Centrifuge ............................................................................................... 4-2
Lid Opening ............................................................................................................ 4-2
Close Lid ................................................................................................................. 4-2
Rotor Installation ..................................................................................................... 4-3
Entering Parameters ................................................................................................. 4-4
Centrifugation ....................................................................................................... 4-10
Temperature Adaptation during Standstill ............................................................. 4-11
Short-term Centrifugation ..................................................................................... 4-11
Removing the Rotor .............................................................................................. 4-12
Aerosol-tight Rotors ............................................................................................... 4-12
Switching the Centrifuge off .................................................................................. 4-12
Table of contents

Table of contents
ii Legend X1 / X1R Thermo Scientific
Chapter 5 System Menu .......................................................................................................................... 5-1
Chapter 6 Maintenance and Care ......................................................................................................... 6-1
Cleaning Intervalls ................................................................................................... 6-2
Cleaning .................................................................................................................. 6-2
Disinfection ............................................................................................................. 6-3
Decontamination ..................................................................................................... 6-4
Autoclaving .............................................................................................................. 6-5
Service of Thermo Fisher Scientific .......................................................................... 6-5
Chapter 7 Troubleshooting ..................................................................................................................... 7-1
Mechanical Emergency Lid Release .......................................................................... 7-2
Troubleshooting by User ......................................................................................... 7-3
When to contact Customer Service .......................................................................... 7-6
Chemical Compatibility Chart ..............................................................................A-1
Contact Information ................................................................................................B-1

Thermo Scientific Legend X1 / X1R iii
P
Preface
Before starting to use the centrifuge, read through this instruction manual carefully and follow the
instructions.
The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is
forbidden to copy or pass on this information without explicit approval.
Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the sellers warranty.
Scope of Supply
If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.Thermo
Fisher Scientific
Intended Purpose
•This centrifuge is an IVD accessory, and therefore subject to the Directive 98/79/EC of the
European Parliament and the Council of October 27, 1998 on in vitro diagnostic medical devices.
•This centrifuge is a laboratory product designed to separate components by generation of Relative
Centrifugal Force. It separates human samples (e.g. blood, urine and other body fluids) collected
in appropriate containers, either alone or after addition of reagents or other additives.
•As general-purpose centrifuge, it is designed to also run other containers filled with chemicals,
environmental samples and other non-human body samples.
•This centrifuge should be operated by trained specialists only.
Article Number Quantity Check
Centrifuge Sorvall Legend X1/X1R 1
Power supply cable 1
50120979 Instruction manual 1
70009824 Anti-corrosion oil 1

Preface
iv Legend X1 / X1R Thermo Scientific
Accident Prevention
Prerequisite for the safe operation of the Sorvall Legend X1/X1R is a work environment in compliance
with standards, directives and trade association safety regulations and proper instruction of the user.
The safety regulations contain the following basic recommendations:
•Maintain a radius of at least 30 cm around the centrifuge.
•Implementation of special measures which ensure that no one can approach the centrifuge for
longer than absolutely necessary while it is running.
•The mains plug must be freely accessible at all times. Pull out the power supply plug or disconnect
the power supply in an emergency.
Precautions
In order to ensure safe operation of the Sorvall Legend X1/X1R, the following general safety
regulations must be followed:
•Do not manipulate the safety devices
•The centrifuge should be operated by trained specialists only.
•The centrifuge is to be used for its intended purpose only.
•Plug the centrifuge only into sockets which have been properly grounded.
•Do not move the centrifuge while it is running.
•Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher
Scientific. Exceptions to this rule are commercially available glass or plastic centrifuge tubes,
provided they have been approved for the speed or the RCF value of the rotor.
•Do not use rotors which show any signs of corrosion and/or cracks.
•Do not touch the mechanical components of the rotor and do not make any changes to the
mechanical components.
•Use only with rotors which have been properly installed. Follow the instructions on the Autolock
in section “Rotor Installation” on page 4-3.
•Use only with rotors which have been loaded properly. Follow the instructions given in the rotor
manual.
•Never overload the rotor. Follow the instructions given in the rotor manual.
•Never start the centrifuge when the lid is open.
•Never open the lid until the rotor has come to a complete stop and this has been confirmed in the
display.

Preface
Thermo Scientific Legend X1 / X1R v
•The lid emergency release may be used in emergencies only to recover the samples from the
centrifuge, e.g. during a power failure (see section “Mechanical Emergency Lid Release” on
page 7-2).
•Never use the centrifuge if parts of its cover panels are damaged or missing.
•Do not touch the electronic components of the centrifuge or alter any electronic or mechanical
components.
•Please observe the safety instructions.
Please pay particular attention to the following aspects:
•Location: well-ventilated environment, set-up on a level and rigid surface with adequate
load-bearing capacity.
•Rotor installation: make sure the rotor is locked properly into place before operating the
centrifuge.
•Especially when working with corrosive samples (salt solutions, acids, bases), the accessory parts
and vessel have to be cleaned carefully.
•Always balance the samples.
Centrifuging hazardous substances:
•Do not centrifuge explosive or flammable materials or substances which could react violently with
one another.
•The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an
explosion-prone environment.
•Do not centrifuge inflammable substances.
•Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without
suitable safety precautions.
When centrifuging microbiological samples from the Risk Group II (according to the Bio-safety
Manual" of the World Health Organization WHO), aerosol-tight biological seals have to be used.
For materials in a higher risk group, extra safety measures have to be taken.
•If toxins or pathogenic substances have gotten into the centrifuge or its parts, appropriate
disinfection measures have to be taken (see “Disinfection” on page 6-3).
•Highly corrosive substances which can cause material damage and impair the mechanical stability
of the rotor, should only be centrifuged in corresponding protective tubes.
IF A HAZARDOUS SITUATION OCCURS, TURN OFF THE POWER SUPPLY TO THE
CENTRIFUGE AND LEAVE THE AREA IMMEDIATELY.
Normal use
The centrifuge is to be used for separating materials of different density or particle size suspended in a
liquid.
Maximum sample density at maximum speed: 1,2 g
cm3
-----------

Preface
vi Legend X1 / X1R Thermo Scientific
This symbol refers to general hazards.
ATTENTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.

Thermo Scientific Legend X1 / X1R 1-1
1
Introduction and Description
Contents
•“Characteristics of the Sorvall Legend X1/X1R” on page 1-2
•“Technical Data” on page 1-3
•“Functions and Features” on page 1-5
•“Rotor Selection” on page 1-6

1Introduction and Description
Characteristics of the Sorvall Legend X1/X1R
1-2 Legend X1 / X1R Thermo Scientific
Characteristics of the Sorvall Legend X1/X1R
The Sorvall Legend X1/X1R is an in-vitro diagnostics device according to the In-Vitro Diagnostics
Directive 98/79/EC.
Several rotors with a wide range of tubes can be used.
The set speed is reached within seconds. The maintenance-free induction motor ensures quiet and
low-vibration operation even at high speeds, and guarantees a very long lifetime.
The user-friendly control panel makes it easy to pre-set the speed, RCF value, running time,
temperature, and running profile (acceleration and braking curves). You can choose between the
display of speed and RCF or the entry mode.
These settings can be changed even while the centrifuge is running.
With the help of the PULSE-key , you can also centrifuge a sample for just a few seconds, if called
for.
The Sorvall Legend X1/X1R is equipped with various safety features:
•The housing and rotor chamber consist of steel plate, the interior of armour steel, while the front
panel is made of high-impact resistant plastic.
•The lid is equipped with a view port and a lock.
•The lid of the centrifuge can only be opened while the centrifuge is switched on and the rotor
has come to a complete stop. The centrifuge cannot be started until the lid has been closed
properly.
•The integrated rotor detection systems ensures that no inadmissible speed settings can be
preselected.
•Electronic imbalance recognition
•Lid emergency release: For emergencies only, e.g. during power failures (see “Mechanical
Emergency Lid Release” on page 7-2)

1 Introduction and Description
Technical Data
Thermo Scientific Legend X1 / X1R 1-3
Technical Data
The technical data of the Sorvall Legend X1/X1R is listed in the following table.
Table 1-1. Technical Data Sorvall Legend X1
Feature Value
Environmental conditions -Use in interior spaces
-Altitudes of up to 2,000 m above
sea level
-max. relative humidity 80% up to
31 °C; decreasing linearly up to
50% relative humidity at 40 °C.
permissible ambient temperature +2° C to +35° C +2° C to +35° C +2° C to +35° C
Overvoltage category II II
Pollution degree 2 2
Heat dissipation refrigerated ventilated
230V 120V 230V 120V
4778 BTU/h 4096 BTU/h 3447 BTU/h 2901 BTU/h
IP 20 20
running time unlimited unlimited
maximum speed nmax 15200 rpm 15200 rpm
minimum speed nmax 300 rpm 300 rpm
maximum RCF value at nmax 25830 xg 25830 xg
maximum kinetic energy <62,5 kJ <51,7 kJ
noise level at maximum speed <63 dB(A) <63 dB(A)
temperature setting range -10 °C to +40 °C
Dimensions refrigerated ventilated
Height
Height with lid open
Width
Depth
Table top height
360 mm
870 mm
623 mm
605 mm
310 mm
360 mm
870 mm
440 mm
605 mm
310 mm
weight without rotor 91.5 kg 57.5 kg

1Introduction and Description
Directives, Standards and Guidelines
1-4 Legend X1 / X1R Thermo Scientific
Directives, Standards and Guidelines
Table 1-2. Directives, standards and guidelines
Tension /
Frequency
Produced and inspected according to the following
standards and guidelines
230V 50/60Hz 2006/95/EC Low Voltage Directive:
2006/42/EC & 98/37/EC Machine Directive:
2004/108/EC EMC Directive
98/79/EC In-vitro-Diagnostika (IvD)
EN 61010-1, 2nd Edition
EN 61010-2-020, 2nd Edition
EN 61010-2-101
EN 61326-1
EN 61326-2-6
EN 55011B
EN 61000-6-2
EN ISO 13485
230V 60Hz
120V 60Hz
100V 60Hz
The centrifuges are produced and inspected
according to the following standards and
guidelines
UL 61010-1, 2nd Edition
CAN/CSA-C22.2 No. 61010-1, 2nd Edition
IEC 61010-2-20, 2nd Edition
(Pollution degree 2, Overvoltage category II)
IEC 61010-2-101
Emitted interference FCC Part 15 CLASS A
NOTE: This equipment has been tested and found to comply with
the limits for a Class A digital device, pursuant to Part 15 of the FCC
Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can
radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful
interference to radio communications. Operation of this equipment
in a residential area is likely to cause harmful interference in which
case the user will be required to correct the interference at his own
expense.
100V 50Hz IEC 61010-1 2nd Edition
IEC 61010-2-020 2nd Edition
IEC 61010-2-101
EN 61326-1
EN 61326-2-6
EN 55011A
EN 61000-6-2
EN ISO 13485

1 Introduction and Description
Functions and Features
Thermo Scientific Legend X1 / X1R 1-5
Functions and Features
The following table gives an overview of the important functional and performance characteristics of
the Sorvall Legend X1/X1R.
Mains Supply
The following table contains an overview of the electrical connection data for the Sorvall Legend
X1/X1R. This data is to be taken into consideration when selecting the mains connection socket.
Table 1-3. Functions and features
Component / Function Description / Features
Structure / Housing Galvanized steel chassis with armoured plating
Chamber Stainless steel
Drive Induction drive without carbon brushes
Keys and display Easy-to-clean keypad and display surface
Controls Microprocessor-controlled
Internal memory The most recent data is saved
Functions RCF-selection, temperature control, pretemp with cooling equipped devices
Acceleration / braking profiles 9 acceleration and 10 braking curves
Rotor recognition Automatic
Imbalance recognition Electronic, contingent on rotor and speed
Lid lock Automatic lid closing and locking starting from an initial hold position
Table 1-4. Electrical connection data of the Sorvall Legend X1/X1R
Cat. # Mains
voltage Frequency Rated current
Power
consumption
Equipment
fuse
Building
fuse
7500 4260 refrigerated 230 V 50 / 60 Hz 8 A 1400 W 15 AT 16 AT
7500 4261 refrigerated 120 V 60 Hz 12 A 1200 W 15 AT 15 AT
7500 4263 refrigerated 100 V 50 / 60 Hz112,5 A 1000 W 15 AT 15 AT
7500 4220 ventilated 230 V 50 / 60 Hz 6 A 1010 W 15 AT 16 AT
7500 4221 ventilated 120 V 60 Hz 9,5 A 850 W 15 AT 15 AT
7500 4223 ventilated 100 V 50 / 60 Hz 9,5 A 750 W 15 AT 15 AT
1Please contact the Thermo Fisher service to run the unit at 60Hz Line frequency

1Introduction and Description
Rotor Selection
1-6 Legend X1 / X1R Thermo Scientific
Rotor Selection
The Sorvall Legend X1/X1R is supplied without a rotor.
Various rotors are available to choose from.
The technical data of the rotors and the corresponding adapters and reduction sleeves for various
commercially available containers can be found in the corresponding rotor operating manuals.
For more information visit our website at: http://www.thermo.com
TX-400 7500 3629
with round buckets 7500 3655
TX-200 7500 3658
with round buckets 7500 3659
BIOShieldTM 720 7500 3621
M-20 7500 3624
Microliter 30x2 sealed 7500 3652
CLINIConicTM 30x15 7500 3623
8x50 sealed 7500 3694
FIBERLiteTM F15-6x100 7500 3698
FIBERLiteTM F13-14x50c 7500 3661
FIBERLiteTM F15-8x50c 7500 3663
FIBERLiteTMM F21-48x2 7500 3664
Microliter 48x2 sealed 7500 3602
HIGHConicTM II 7500 3620

Thermo Scientific Legend X1 / X1R 2-1
2
Before use
Contents
•“Before Setting up” on page 2-2
•“Transporting the Centrifuge” on page 2-2
•“Location” on page 2-2
•“Aligning the Centrifuge” on page 2-3
•“Storage” on page 2-4

2Before use
Before Setting up
2-2 Legend X1 / X1R Thermo Scientific
Before Setting up
1. Check the centrifuge and the packaging for any shipping damage.
Inform the shipping company and Thermo Fisher Scientific immediately if any damage is
discovered.
2. Remove the packaging.
3. Check the order for completeness (see “Scope of Supply” on page iii).
If the order is incomplete, please contact Thermo Fisher Scientific.
Transporting the Centrifuge
•Due to its weight (see “Technical Data” on page 1-3), the centrifuge should be carried by several
people.
•Always lift the centrifuge at both sides.
Figure 2-1. Lifting the centrifuge at both sides.
•The centrifuge can be damaged by impacts.
•Transport the centrifuge upright and if at all possible in its packaging.
Location
The centrifuge should only be operated indoors.
The set-up location must fulfil the following requirements:
•A safety zone of at least 30 cm must be maintained around the centrifuge.
People and hazardous substances must be kept out of the safety zone while centrifuging.
WARNING Always lift the centrifuge on both sides.
Never lift the centrifuge by its front or the back panel.
Always remove the rotor before moving the centrifuge.

2 Before use
Aligning the Centrifuge
Thermo Scientific Legend X1 / X1R 2-3
•The supporting structure must be stable and free of resonance, for example a level laboratory
bench.
•The supporting structure must be suitable for horizontal setup of the centrifuge.
•The centrifuge should not be exposed to heat and strong sunlight.
•The set-up location must be well-ventilated at all times.
Aligning the Centrifuge
The horizontal alignment of the centrifuge must be checked every time after moving it to a different
location.
The supporting structure must be suitable for horizontal setup of the centrifuge.
Mains Connection
Figure 2-2. Mains connections
1. Turn off the power supply switch on the back (press "0").
2. Plug the centrifuge into grounded electrical sockets only.
3. Check whether the cable complies with the safety standards of your country.
WARNING UV rays reduce the stability of plastics.
Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.
CAUTION If the centrifuge isn’t level, imbalances can occur and the centrifuge can be
damaged.
Do not place anything under the feet to level the centrifuge.
Main Power Switch
Mains plug

2Before use
Storage
2-4 Legend X1 / X1R Thermo Scientific
4. Make sure that the voltage and frequency correspond to the figures on the rating plate.
Figure 2-3. Rating plate of the Sorvall Legend X1R
5. Establish the connection to the power supply with the connecting cable.
Storage
•Before storing the centrifuge and the accesories it must be cleanedand if necessary desinfected
and decontaminated.
•Store the centrifuge in a clean, dust-free location.
•Be sure to place the centrifuge on its feet.
•Avoid direct sunlight.