Hygiena RiboPrinter User manual

DNA Prep Carrier
3 Inspect wells to ensure they are not empty:
1. Make sure most of the blue liquid is at the bottom of the well.
2. Make sure there are no air bubbles.
3 Gently shake down the carrier to release any liquid that may adhere to the lid.
DNA Prep Enzyme Vial
3 Tap the vial to release any liquid that may have adhered to the cap.
3 Remove the cap.
3 Press the vial firmly into the DNA Prep Carrier.
Membrane
3 Touch only the black frame around the membrane.
3 Hold the black frame with the membrane facing you and the cut-out square in the upper-left corner of the frame.
3 Load the membrane into the front slot of the S/T chamber.
MP Base
3 Make sure the base has been incubated at 37°C for 24 hours.
3 Make sure each well in the base is nearly full of liquid and the liquid levels are relatively consistent.
3 Invert each insert 3-5 times to loosen the powder.
3 Push each insert firmly into position so that no parts extend above the base.
Gel Cassette
3 Open the foil packet and check that a small amount of buffer (about 1 mL) is present in the packet. If no buffer is present,
use a different gel.
3 Slowly remove the rubber comb from the cassette.
3 Fold the handle of the cassette toward you. You will hear an audible click when the handle snaps into place.
3 Inspect the gel for anomalies (bubbles, shrinkage, excess agarose or uneven lanes). If any of these are present, use a
different gel.
RiboPrinter®Operations Ready Reference

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Batch Loading Time Chart Load up to 4 batches in an 8-hour shift
Start Batch 1 @ 8:00 AM
RR-007 Rev 01
Start Batch 2 in 2hrs @ 10:00 AM
Start Batch 3 in 2:20hrs @ 12:20 PM
Start Batch 4 in 3 hrs @ 3:20 PM
Start Batch 5 in 3 hrs @ 6:20 PM
Start Batch 6 in 3 hrs @ 9:20 PM
8:00 AM 10:00 AM 12:00 PM 2:00 PM 4:00 PM 6:00 PM 8:00 PM 10:00 PM 12:00 AM 2:00 AM 4:00 AM