Hygiena BAX System X5 User manual

User Guide

© Copyright 2017 Qualicon Diagnostics LLC, a Hygiena™ Company or its affiliates. All rights reserved worldwide. No part of this
publication may be reproduced or transmitted in any form, or by any information storage and retrieval system, without the prior
agreement and written permission of Hygiena.
Information in this document is subject to change without notice. Hygiena assumes no responsibility for any errors that may appear in
this document. This document is believed to be complete and accurate at the time of publication. In no event shall Hygiena be liable
for incidental, special, multiple, or consequential damages in connection with or arising from the use of this document.
Part Number: UG-002 Rev 01 v1.3
Effective Date: 29 AUG 2017
TRADEMARKS
The Hygiena™ logo and BAX® are trademarks or registered trademarks of Hygiena or its affiliates. SYBR is a registered trademark
of Thermo Fisher Scientific or its subsidiaries in the U.S. and/or certain other countries. MATLAB is a registered trademark of The
Mathworks, Inc. Microsoft Windows® is a registered trademark of Microsoft Corporation. All other trademarks are property of their
holders.
FIELD OF USE
Data obtained from the BAX® System should not be used for human diagnostic or human treatment purposes. Equipment is not
approved by the United States Food and Drug Administration or any other U.S or non-U.S. regulatory agency for use in human
diagnostics or treatment. The BAX® System should not be used as the sole basis for assessing the safety of products for release to
consumers. The information generated is only to be used in conjunction with the user’s regular quality assurance program. Use for
research and development, quality assurance and quality control under supervision of technically qualified persons. Read the
Limitation of Warranty and Liability before using product.
LICENSING
MATHWORKS
MATLAB 1984-2005 The MathWorks, Inc. is deployed with the BAX® System application and governed bya certain license agreement
between Hygiena and MathWorks. License agreement files (MathWorks or Third-Party) that are included with the MCR Libraries
(“MCR Library License”) may not be modified or removed.
THERMO FISHER SCIENTIFIC
The SYBR® Green I stain in some BAX® System PCR assays is sold under license from Life Technologies Corporation, a Thermo
Fisher Scientific Company, for use only in homogenous polymerase chain reaction assays in combination with the DNA detection
technology of Hygiena, and is covered by pending and issued patents.
PROMEGA CORPORATION
GoTaq® Hot Start Polymerase in some BAX® System PCR assays is sold under distribution agreement with Promega Corporation.
GoTaq® Hot Start Polymerase is manufactured by and licensed to Promega Corporation under U.S. Patent Nos. 5,338,671 and
5,587,287 and their corresponding foreign patents and sold to Hygiena for distribution.

CONTENTS
Introduction and Overview ......................................................................................................................................1
About the BAX® System.........................................................................................................................................1
User Assumptions...................................................................................................................................................2
Text Conventions....................................................................................................................................................2
Ordering Information...............................................................................................................................................3
Other Required Equipment and Supplies................................................................................................................4
Storage and Disposal Information...........................................................................................................................5
Shipping Instructions...............................................................................................................................................6
Technical Assistance..............................................................................................................................................6
I. The BAX® System X5 Instrument........................................................................................................................7
Instrument Specifications........................................................................................................................................7
Environmental Parameters......................................................................................................................................7
Instrument Components..........................................................................................................................................8
Safety Precautions..................................................................................................................................................9
Instrument Installation...........................................................................................................................................11
Instrument Configuration.......................................................................................................................................11
Instrument Maintenance .......................................................................................................................................13
Instrument Decontamination.................................................................................................................................13
Capping/Decapping Tools.....................................................................................................................................15
II. The BAX® System X5 Software........................................................................................................................17
The BAX® System X5 Software Window..............................................................................................................17
Creating a Rack File .............................................................................................................................................19
Printing Reports....................................................................................................................................................20
Installing and Upgrading the BAX® System Software...........................................................................................22
III. The BAX® System X5 PCR Assays.................................................................................................................24
BAX® System Assay, Assay Validation................................................................................................................24
Using the E. coli O157:H7 Assay........................................................................................................................255
Using the Genus Listeria Assay..........................................................................................................................311
Using the L. monocytogenes Assay....................................................................................................................377
Using the Salmonella Assay ...............................................................................................................................433
Reviewing BAX® System Results.........................................................................................................................50
Best Practices for Sample Enrichment and Preparation………………………………………………………………..52
Using Analog Heating and Cooling Blocks with the BAX® System Assays ........................................................524
IV. Troubleshooting the BAX® System...............................................................................................................555
Accessing Log Files............................................................................................................................................555

Troubleshooting Indeterminate or Error Results .................................................................................................555
Troubleshooting Process Interruptions ...............................................................................................................577

1 BAX® System X5 User Guide
Introduction and Overview
About the BAX® System
The BAX®System is an automated molecular detection method for identifying foodborne pathogens, spoilage
organisms and other microbes in raw ingredients, finished products and environmental samples. For more than 20
years, food companies, service labs and government regulators around the world have relied on the BAX® System to
help them get products to market faster while protecting both quality and reputation.
The BAX® System was the first molecular food testing method to use Polymerase Chain Reaction (PCR) technology,
which rapidly creates millions of copies of the targeted DNA fragment, if present, in a very short time. The result is a
clearly detectable “yes-or-no” answer within hours of starting the assay, without the need for expert interpretation.
The BAX® System combines the power of PCR with automated detection to significantly reduce hands-on time,
minimize the potential for cross-contamination, and provide for consistent results based on computerized algorithms
for analysis. Simply load your prepared samples, run the program and read the results on screen.
To further simplify the process, the BAX® System assays provide all requisite PCR primers, polymerase and
nucleotides as a stable, dry, manufactured tablet in sealed PCR tubes to eliminate the need for reagent preparation.
After amplification, these tubes remain sealed for the detection phase, significantly reducing the potential for sample
or laboratory contamination with the amplified PCR product.
About the Polymerase Chain Reaction (PCR)
PCR is an analytical tool for quickly replicating a targeted DNA fragment. Each BAX® System assay targets specific
DNA fragments unique to the organism of interest, providing a stable, reliable indicator that the organism is present
and vastly reducing the potential for cross reactions with similar organisms.
In a typical application, sample DNA is combined with DNA polymerase, nucleotides and primers that are specific for
a given nucleotide sequence. This mixture then goes through a series of timed heating and cooling cycles. Heating
denatures the DNA, separating it into single strands. As the mixture cools, the primers recognize and anneal (bind) to
the targeted sequence. DNA polymerase then uses the provided nucleotides to extend the primers, thus creating two
copies of the targeted DNA fragment (amplification). Repeating the cycle of denaturing, annealing, and extending
produces an exponential increase in the number of target DNA fragments, creating millions of copies in a very short
time. If the target sequence is not present, no detectable amplification takes place.
About Automated Detection
As the target DNA is amplified through PCR in the BAX® System instrument, fluorescent dye in the BAX® System
PCR tablets binds to the double-strands, causing it to emit a fluorescent signal. After amplification, the BAX® System
begins a detection phase where the fluorescent signal is measured. During detection, the temperature of the samples
is raised to the point where the DNA strands separate (denature), releasing the dye and lowering the signal. This
change in fluorescence is plotted against temperature to generate a melting curve, which is interpreted by the BAX®
System software as positive or negative results.

Introduction and Overview
2 BAX® System X5 User Guide
User Assumptions
•Users are qualified lab personnel who follow good microbiology laboratory practices, including the safe
handling and disposal of potentially pathogenic materials.
•Users are familiar with Microsoft® Windows® operating system, including navigation and backup
procedures.
Text Conventions
This manual uses the following text conventions to indicate software commands/selections/messages:
•Menu selections appear in UPPER CASE separated by > to indicate sequence.
•Field names and screen names appear in bold lower case.
•Screen buttons appear in upper case in a BOX
•System messages appear in “quotes.”
•Notes appear in italics.
•Safety symbols are detailed in Chapter I, but the main categories are as follows:
General alert or warning
Hot surface! Avoid touching.
Live power may still be available even with power off or failed fuse.

Introduction and Overview
BAX® System X5 User Guide 3
Ordering Information
For more information about the BAX® System and other products from Qualicon Diagnostics, contact your local
representative or visit www.hygiena.com.
Note: The BAX® System X5 instrument is designed for use with BAX® System X5 assays and associated materials only. BAX®
System Q7 assays cannot be used with the BAX® System X5 instrument.
BAX® System Equipment and Supplies
Part Number
Description
Contents
MCH2005
(D15407117)
BAX® System X5 Instrument
Package
BAX® System X5 instrument
BAX® System X5 reference card
Bag of empty, white PCR tubes for sample balancing (96)
Power supply and cables
Network cable
USB memory drive with user documentation and software
Flight case for transport/shipping
MCH2003
(D15407134)
BAX® System X5 Computer
Package
Laptop computer pre-loaded with BAX® System X5
software
Computer mouse
Security cable lock
MCH2000
(D15407120)
BAX® System X5 Auxiliary
Package
* Analog heating and cooling
blocks may be used in place of
the Automated Thermal Block.
See “Using Analog Heating and
Cooling Blocks with the BAX®
System Assays”for details.
Automated Thermal Block with metal tube holders and
power supply
32-well aluminum cold block with insulator for PCR tubes
32-well sample carriers (2)
Capping/decapping tools (2)
Multi-channel pipette (1)
Adjustable mechanical pipettes (2)
Repeating pipette (1)
Pipette tips with barriers
Cluster tubes with caps and racks
Powder-free nitrile gloves
BAX® System Enrichment Media
Part Number
Description
Contents
MED2016
(D12745725)
BAX® System MP Media -
StatMedia™ soluble packets
20 pouches x 5 packets per pouch x 33.75 g per packet
MED2003
(D12404925)
BAX® System MP Media -
bulk powder
2.5-kg tub
MED2002
(D12232794)
BAX® System Media for
Listeria - bulk powder
2.5-kg tub

Introduction and Overview
4 BAX® System X5 User Guide
BAX® System X5 PCR Assays
Part Number
Description
Contents
KIT2022
(D15407214)
BAX® System X5 PCR Assay
for E. coli O157:H7
Lysis buffer (2 x 12 mL)
Protease (400 µL)
PCR tubes with tablets (64)
Flat optical caps for PCR tubes (96)
KIT2024
(D15407193)
BAX® System X5 PCR Assay
for Genus Listeria
Lysis buffer (2 x 12 mL)
Protease (400 µL)
Lysing agent 2 (1.1 mL)
PCR tubes with tablets (64)
Flat optical caps for PCR tubes (96)
KIT2023
(D15407201)
BAX® System X5 PCR Assay
for L. monocytogenes
Lysis buffer (2 x 12 mL)
Protease (400 µL)
Lysing agent 2 (1.1 mL)
PCR tubes with tablets (64)
Flat optical caps for PCR tubes (96)
KIT2025
(D15407187)
BAX® System X5 PCR Assay
for Salmonella
Lysis buffer (2 x 12 mL)
Protease (400 µL)
PCR tubes with tablets (64)
Flat optical caps for PCR tubes (96)
Other Required Equipment and Supplies
In addition to the BAX® System X5 instrument, start-up package, and PCR assays, the following items are required
to use the BAX® System method. These items are not supplied by Hygiena.
•Stomacher capable of homogenizing samples between 200-260 rpm (or equivalent)
•Filtered stomacher bags
•Incubators capable of maintaining designated temperatures within ±2°C
•Hypochlorite bleach (10% household solution)
•Standard solutions, consumables and media

Introduction and Overview
BAX® System X5 User Guide 5
Storage and Disposal Information
Equipment Storage
•Clean and maintain required equipment according to the instructions of the manufacturer.
•Cooling blocks should be kept refrigerated at 2-8°C and used within 30 minutes of removal from refrigerator.
•Pipettes should be calibrated to deliver within 10% of required volumes. Barrier tips are recommended for all
pipettes.
Assay Storage
•Keep BAX® System reagents and unused PCR tubes refrigerated at 2–8°C. Do not freeze.
•If storing PCR tubes with tablets in an open kit for more than 3 weeks, seal the Mylar bag of PCR tubes into
a larger bag with desiccant or store at 4ºC in a desiccation unit, if possible.
Reagent Storage
•Reagents should be used by the expiration date stamped on the individual labels.
•After protease has been added to the lysis buffer, shelf life of the solution is two weeks when stored at 2-
8°C.
•For BAX® System X5 Listeria assays, lysis reagent made with protease, Lysing Agent 2 and lysis buffer can
be stored for up to one week at 2-8°C

Introduction and Overview
6 BAX® System X5 User Guide
Shipping Instructions
In the event your BAX® System X5 instrument requires service, contact Hygiena or your local distributor to obtain a
return authorization number and instructions for returning the unit:
•Thoroughly clean the unit of any biohazardous substances.
•To avoid damage to the instrument, use ONLY the BAX® System X5 flight case for shipping the instrument.
•Ship the unit to the address on the return form. Purchasing insurance from your shipper is strongly
recommended.
Technical Assistance
If you have any questions or comments on the BAX® System, contact your local distributor or Hygiena representative
for technical assistance.
You can also contact Hygiena Diagnostics Support directly at [e-mail].
Hygiena Diagnostics Support
United States and Canada
Phone: [phone]
Fax: [phone]
Outside the United States and Canada
Phone [phone]

BAX® System X5 User Guide 7
I. The BAX® System X5 Instrument
Instrument Specifications
Item
Specification
Dimensions (width × depth × height)
9.45 in × 10.63 in × 9.06 in (24 cm × 27 cm × 23 cm)
Weight
15.4 lbs (7 kg)
Power supply
100-240 VAC; 50-60 Hz
Power usage
Operates at 24 V with 6.25 A; maximum usage 170 VA
Mains power consumption
170 W
Noise level
< 40 dB (A)
Heat output during run (mean value)
~58 Btu/h or 170 kJ/h
Heat output in standby
~34 Btu/h or 36 kJ/h
Sample capacity
Up to 32 samples per run
Processing time
3.5 hours for standard PCR protocols
Fluorescence spectrometer excitation
Filtered LED, 500 nm
Fluorescence spectrometer detection
Spectrometer, 2 nm (emission wavelength)
Channels
120 optical channels
Temperature range
40-99°C
Heating rate
4°C/s
Cooling rate
3°C/s
Thermal homogeneity
±0.25°C
Thermal accuracy
±0.50°C
Environmental Parameters
Item
Specification
Minimum bench space required (width × depth × height)
9.45 in × 12.21 in × 14.96 in (24 cm × 31 cm × 38 cm)
Temperatures allowed during transportation, storage and
packaging
-20 to +60°C
Relative humidity allowed during transportation, storage
and packaging
10-95%, no condensation
Altitude/pressure allowed during transportation, storage
and packaging
0-3,000 m above sea level / 70-106 kPa
Temperatures allowed during operation
15-32°C
Relative humidity allowed during operation
Max. 80% at 32°C, no condensation / Min. 30% at 15-32°C
Altitude/pressure allowed during operation
0-2,000 m above sea level / 80-106 kPa

Chapter I - The BAX® System X5 Instrument
8 BAX® System X5 User Guide
Instrument Components
Instrument Lid
The lid of the BAX® System X5 instrument shows different colors based on the status of the instrument:
When instrument is not processing samples
Color
Instrument Status
Blue (pulsing)
The instrument is powering on.
Red (steady)
The instrument lid is open.
Red (flashing)
An instrument error is detected.
During a process run
Color
Instrument Status
Blue (steady)
The instrument is pre-heating for a process run.
Green (steady)
The instrument is ready for a process run. Samples are not loaded yet.
Red (pulsing)
The instrument is pre-heating during a process run.
Red and Blue
(alternating)
The instrument is alternating between heating and cooling samples during a
process run.
Blue (pulsing)
The instrument is acquiring optical data during a process run
Cyan (steady)
The process run is complete. Samples can be removed from the instrument.
Instrument Mount
The instrument mount accepts up to four strips of eight BAX® System X5 PCR tubes each or up to 32 single tubes.
The rows and columns of the instrument mount are numbered according to the display in the plate view.

Chapter I –The BAX® System X5 Instrument
BAX® System X5 User Guide 9
If you are processing fewer than 32 samples in the BAX® System X5 instrument, it is important to load samples in a
symmetric pattern to ensure the instrument is balanced and to prevent potential damage to the instrument lid. We
recommend loading samples first in Row A and Row D, starting at the corners. Once the two outer rows are full, then
use Row B and Row C to load samples in a symmetric pattern.
Note: BAX® System Q7 PCR tubes cannot be used with the BAX® System X5 instrument.
Safety Precautions
Instrument Safety
WARNING - PHYSICAL INJURY HAZARD. Use this instrument as specified by Hygiena to avoid personal
injury or damage to the instrument
DANGER - ELECTRICAL HAZARD. Use properly configured and approved line cords for the voltage supply
in your facility. Use only the power cables and Ethernet connector supplied with the system package.
Caution! Any break in the electrical ground wire, whether inside or outside the instrument, or disconnection of
the electrical ground connection, could create a hazardous condition.
Caution! Do not under any circumstances attempt to modify or deliberately override the safety features of this
system.
The BAX® System X5 instrument is for indoor use only.
Do not place the instrument in direct sunlight or close to radiators or heating devices.
Do not place the BAX® System X5 instrument next to any source of vibration or electromagnetic interference, or
instrumentation that has high inductance (e.g., refrigerators, centrifuges, or mixers).
Do not place anything on top of the instrument.
Do not to place anything under the instrument, such as sheets of paper, since these could block the air inlet.

Chapter I - The BAX® System X5 Instrument
10 BAX® System X5 User Guide
Biological Safety
Samples analyzed with this instrument may contain potentially bio-hazardous material such as bacterial, fungal
or viral agents. As with all potentially biohazardous specimens, universal safety precautions should be taken when
handling and processing samples.
The BAX® System method includes sample preparation enrichment procedures that nourish the growth of
potential pathogens to detectable levels. Because pathogens can cause human illness, appropriate safety
precautions must be taken when handling samples, media, reagents, glassware and other supplies and equipment
that could be contaminated with potentially pathogenic bacteria. In particular, individuals who are pregnant or
immunocompromised should avoid any potential contact with Listeria monocytogenes.
Reagents used with the BAX® System assays should pose no hazards when used as directed. Before using this
product, please review the Safety Data Sheets (SDS), available on the web at www.Hygiena.com. Refer to your site
practices for safe handling of materials at extreme temperatures.
Wear protection gloves and clothing.
Never eat, drink, or smoke in the laboratory.
Wash your hands before and after working.
Safety and Electromagnetic Capability (EMC) Standards
U.S. and
Canadian Safety
Standards
This instrument has been tested to and complies with standard UL 3101-1,
“Safety Requirements for Electrical Equipment for Laboratory Use, Part 1:
General Requirements
This instrument has been tested to and complies with standard CSA 1010.1,
“Safety Requirements for Electrical Equipment for Measurement, Control, and
Laboratory Use, Part 1: General Requirements.”
Canada EMC
Standard
This instrument has been tested to and complies with ICES-001, Issue 3:
Industrial, Scientific, and Medical Radio Frequency Generators
European Safety
and EMC
Standards
Safety
This instrument meets European requirements for safety (Low Voltage Directive
73/23/EEC). This instrument has been tested to and complies with standards EN
61010-1:2001, “Safety Requirements for Electrical Equipment for Measurement,
Control and Laboratory Use, Part 1: General Requirements,” and EN 61010-2-
010, “Particular Requirements for Laboratory Equipment for the Heating of
Materials.”
EMC
This instrument meets European requirements for emission and immunity (EMC
Directive 89/336/EEC). This instrument has been tested to and complies with
standard EN 61326 (Group 1, Class B), “Electrical Equipment for Measurement,
Control and Laboratory Use –EMC Requirements.”
Australian EMC
Standards
This instrument has been tested to and complies with standard AS/NZS 2064,
“Limits and Methods Measurement of Electromagnetic Disturbance
Characteristics of Industrial, Scientific, and Medical (ISM) Radio-frequency
Equipment.”

Chapter I –The BAX® System X5 Instrument
BAX® System X5 User Guide 11
Instrument Installation
Review the Environmental Parameters, Instrument Specifications and Safety Precautions in this
User Guide before installing the BAX® System X5 instrument.
1. Review the laboratory space intended for the BAX® System X5 instrument and confirm that the appropriate
space requirements are met (see “Environmental Parameters” and “Instrument Specifications” for details).
2. Open the flight case. Lift the BAX® System X5 instrument out of the flight case and place it on a solid, level
surface.
3. Lift the bottom panel divider out of the flight case. Check to ensure that all components are present and
intact.
4. Connect one end of the Ethernet cable to the Ethernet port of the BAX® System X5 computer. Connect the
other end of the Ethernet cable to the Ethernet port on the rear of the BAX® System X5 instrument.
Note: A LAN network connection may also be used to connect the BAX® System X5 instrument and computer. To
connect using an existing LAN, use the appropriate IP addresses provided by your network. The connections can be on
any network ports in the same subnet and still allow the software to find the instrument. If necessary, hubs or switches
can be used to create more access points.
5. Connect the AC adapter to the rear of the BAX® System X5 instrument.
6. Keep the flight case and packaging in case of return.
Instrument Configuration
After installing a new BAX® System X5 instrument, you may need to configure the instrument for your software. You
may also need to configure the instrument with your software if you are using multiple BAX® System X5 instruments
with a single computer and switch between instruments.
Ensure that the BAX® System X5
computer is connected to the BAX®
System X5 instrument. When the
instrument has a valid connection, you
will see a green and yellow light above
the connector.
From the BAX® System menu bar, select
TOOLS > CONFIGURE INSTRUMENT…
A dialog box opens to display information
about the instrument.
Under “Available Instruments”, use the
drop-down menu to select the desired
instrument. (In the example photo,
instrument “A81187D2CD” is selected.)
Click OK to confirm the selection.

Chapter I - The BAX® System X5 Instrument
12 BAX® System X5 User Guide
Note: In some cases, the BAX® System
X5 software may detect that the
instrument does not have the latest
firmware version. If a newer firmware
version is available, the dialog box will
display a message above the OK button
that recommends updating instrument
firmware version.
To update the instrument firmware
version, click the UPDATE FIRMWARE
button. A status appears in the dialog
box to display the progress of the update.
After the update is complete, click OK to
confirm the instrument selection.
After configuration, the name of the
selected BAX® System X5 instrument
will appear in the bottom-right corner of
the software screen.

Chapter I –The BAX® System X5 Instrument
BAX® System X5 User Guide 13
Instrument Maintenance
The BAX® System X5 instrument does not require any regular maintenance procedures. If desired, basic cleaning
and upkeep can be performed by following the instructions below.
Check the area around the BAX® System X5 instrument to ensure that the air flow is unrestricted.
Cleaning the Instrument Casing
Remove dust and debris by wiping the instrument surfaces with a lint-free cloth. If needed, the casing can be wiped
off with a damp cloth using mild soap or common disinfectants, such as 70% ethanol. Do not use cleansers
containing iodine or acetone.
Cleaning the Sample Wells
1. Use a lint-free swab soaked in 70% ethanol to gently clean the inside of the wells.
2. Dry the wells with a lint-free cloth of dry swab and remove small particles with an air duster.
Instrument Decontamination
When samples undergo PCR, the amplification process produces billions of copies of a specific DNA sequence from
the targeted bacteria. If tubes are then unsealed, these copies can travel throughout the lab via aerosols, liquids and
surface contact.
The BAX® System avoids this problem by processing only sealed tubes. However, as with any PCR technology, the
risk of contamination cannot be completely eliminated. If the tubes are not properly sealed or are inadvertently
opened after amplification, contamination of the lab should be assumed and care should be taken to minimize
aerosols.
Follow these procedures to remove known amplicon contamination or as instructed by Diagnostics Support.
Decontaminating Sample Wells
1. Dilute a commercially available 5% bleach solution with pure water to create a 1% sodium hypochlorite
solution.
2. Use a lint-free swab moistened with 1% sodium hypochlorite solution to thoroughly wet each sample well.
3. Let the solution sit in the sample wells for 10 minutes.
4. Use a lint-free swab moistened in pure water to wipe each sample well. Be sure to remove the sodium
hypochlorite solution completely.
5. Wipe each sample well with a lint-free swab soaked in 70% ethanol.
6. Dry the wells and remove small particles with an air duster.

Chapter I - The BAX® System X5 Instrument
14 BAX® System X5 User Guide
Decontaminating the Instrument Mount and Exterior Surfaces
1. Dilute a commercially available 5% bleach solution with pure water to create a 1% sodium hypochlorite
solution.
2. Wipe the exterior surfaces of the BAX® System X5 instrument and the mount with a cloth dampened –but
not dripping wet –with 1% sodium hypochlorite solution.
3. Let the solution sit on the instrument for 10 minutes.
4. Use a lint-free cloth moistened in pure water to thoroughly wipe the BAX® System X5 instrument and the
mount. Be sure to remove the sodium hypochlorite solution completely.
5. Use a lint-free cloth soaked in 70% ethanol to wipe the BAX® System X5 instrument and the mount.
6. Let the instrument air-dry for a minimum of 10 minutes before using the instrument.

Chapter I –The BAX® System X5 Instrument
BAX® System X5 User Guide 15
Capping/Decapping Tools
With your purchase of the BAX® System X5 start-up package, you receive a tool* that facilitates the decapping and
resealing of the PCR tubes. This tool allows you to easily remove the domed caps from a strip of up to eight PCR
tubes at once without jarring the contents of the tubes (A). The rounded end is used to reseal the tubes with flat
optical caps using a rolling motion (B).
A.Removing domed caps from PCR tubes
Place tool flange behind row of
caps.
Tilt the tool forward and push
down so that it locks over the
caps.
Tilt the tool backward so that
the caps lift off.
B. Sealing PCR tubes with flat optical caps
Place rounded edge of tool over
first cap.
Roll the tool over the strip to
lock the caps in place.
Visually inspect the caps to
make sure they are seated.
*U.S. patent 5.967.001
Note: A separate capping/decapping tool is provided for use with cluster tubes for sample lysis.