Hygiena Dualo 32 R2 Instruction sheet

Product Instructions
Dualo 32®R2
Product No. MCH230003

Product Instructions:
Revision A, July 2023
Dualo 32® R2
Product No.: MCH230003

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MCH230003 - Dualo 32R2
TABLE OF CONTENTS
TABLE OF CONTENTS
This user guide will teach you what you need to know to start running your Dualo 32®R2
instrument. It describes everything from connecting your instrument to the network to data
analysis for your qPCR experiments.
Contents include
1. SETUP
Learn how to install your Dualo 32 R2 - from setting it up on your workbench,
to configuring the instrument and installing the software.
2. TIPS
Learn some useful tips for using your instrument.
3. EXPERIMENT
Learn the basics of setting up an experiment on your Dualo 32 R2.
4. ANALYSIS
Learn how to easily analyze your results.
5. EXPORT
Learn how to export your data for use with other software.
6. MAINTENANCE
Learn how to look after your Dualo 32 R2.
7. TROUBLESHOOTING
If you ever have a problem with your instrument, learn how to troubleshoot it here.

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MCH230003 - Dualo 32R2
SETUP
1. SETUP
Installing your Dualo 32 R2
You should have the following items in the Dualo 32 R2 flight case:
1. Dualo 32 R2 qPCR Instrument
2. Power Supply Unit and Mains Cable
3. Ethernet Cable
4. USB Drive
Please keep the flight case and outer box the Dualo 32 R2 came in, in case you need to protect
your Dualo 32 R2 during storage or shipment in future.
Lid
The lid ensures that tubes are seated correctly in the wells, provides optical isolation, includes
a heated lid to reduce condensation, and prevents dust falling into empty wells.
USB Port
Insert the USB drive here to run an experiment from the USB drive.
Lid
USB Port

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MCH230003 - Dualo 32R2
Display LEDs
The Dualo 32 R2 uses display LEDs to help you understand what the instrument is doing.
Here is a summary of these display LEDs.
Blue Initializing
Green Idle
Red Lid open - please close the lid
Yellow Tubes loaded
Blue Running, low temperature
Pink Running, medium temperature
Red Running, high temperature
Cyan Experiment complete
Red-Flashing Fault detected
Dualo 32 R2 Connections
The Dualo 32 R2 has three connections. One in the front and two in back as shown below:
This is to connect your Dualo 32 R2 to the
provided power supply unit.
This is for connecting your Dualo 32 R2 to
your LAN or computer.
Ethernet PortPower
SETUP
Ethernet Port
Power

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MCH230003 - Dualo 32R2
Powering on your Dualo 32 R2
Place the Dualo 32 R2 on your lab bench and then connect AC power to the power
supply unit. After a few seconds, your instrument will turn on. The display LEDs will light up
blue and then turn green if a lid is closed or red if not. Your Dualo 32 R2 is ready to run.
The Dualo 32 R2 uses a 3-pin IEC mains connector. If you are not using a grounded supply then
you must provide an additional ground connection.
Connecting your Dualo 32 R2
to a Network, PC or Laptop
Connect one end of the Ethernet cable to the instrument and the other end to a LAN port, or
directly to your computer. Once your Dualo 32 R2 is connected to a LAN, you can connect to
your instrument via Wi Fi if your network supports it.
Dualo 32 R2 Connection Modes
USB
USB
1. Direct network connection
3. USB drive connection
2. Local area network connection
LAN
SETUP

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MCH230003 - Dualo 32R2
Dualo 32 R2 Heated Lid
The Dualo 32 R2 heated lid will get hot. Please do not touch it. The heated lid will be preheated
to 105 ºC if user activity is detected. This enables your run to start as soon as possible. After
5 minutes of inactivity, the heated lid will be turned off to conserve energy.
Installing your Dualo 32 R2 Software
WINDOWS (MAC OS X also available)
Double-click on the Dualo 32 R2-Windows-Installer and follow the on-screen instructions to
install your Dualo 32 R2 software on Windows.
HEATED LID
DO NOT TOUCH
SETUP

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MCH230003 - Dualo 32R2
SETUP

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MCH230003 - Dualo 32R2
Configuring your Dualo 32 R2
Please select Configuration
Please select Dualo 32 R2
To add your new Dualo 32 R2, select the Add button.
You will now be presented with a list of available instruments.
SETUP

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Double-click on the instrument
you wish to connect to, or press
Select with the instrument
selected. The instrument will
now be added to the list of
Registered Instruments for
use in the software.
SETUP