Showa Denko Shodex RSpak DS-413 User manual

Operation Manual Shodex RSpak DS-413 Version 191023E
Operation Manual
Shodex™ RSpak DS-413
Europe, Middle East, Africa, Russia
www.shodex.de
Sales Office:
Showa Denko Europe GmbH
Shodex Business
Konrad-Zuse-Platz 3
81829 Munich
Germany
Technical Support:
[email protected]
+49 (0)89 / 93 99 62 37
Quotes and Orders:
[email protected]
+49 (0)89 / 93 99 62 34

Operation Manual Shodex RSpak DS-413 Version 191023E
2
Operation Manual
Shodex™ RSpak DS-413
(Please read this manual carefully to achieve the best and consistent column performance for a long time)
Important Handling Instructions
Caution! • Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the
column and understand their proper handling methods to prevent potential health
hazards or death from occurring.
• Please wear appropriate personal protective equipment such as lab goggles and
gloves when handling organic solvents and acid and alkaline reagents. Avoid any direct
physical contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag,
and enclosed Certificate of Analysis (CoA) are matching and correct.
1. Introduction
Thank you for purchasing the Shodex™product. Shodex RSpak DS-413 is a polymer-based reversed-
phase chromatography column. It can be used in a wide pH range from acidic to alkaline (pH 1 to 13).
The columns fulfill USP L21 requirements.
2. Column Components
3. HPLC System Preparation
Wash entire LC system prior to the column installation, including all flow-lines and sample loop by
switching the valve, and then replace the washing solution with the eluent to be used. When desired
new eluent has low miscibility/solubility to the eluent of previous analysis, first use the eluent that is
miscible/soluble to both eluents, and then replace it with the desired eluent.
(Ex. 1) When replacing chloroform to acetonitrile/water, first run methanol and then
introduce acetonitrile/water.
(Ex. 2) When replacing buffer solution (or salt solution) to acetonitrile/water, first run 100 %
pure water and then replace it with the desired eluent.

Operation Manual Shodex RSpak DS-413 Version 191023E
3
Attention! • If the eluent left in the system is not compatible with the column to be used, it may
damage the column.
• A drastic change in the eluent compositions may remove substances adsorbed on
the system and they may enter and deteriorate the column.
4. Column Installation
(1) Connect the column to LC system by following the "flow direction arrow" (->) indicated on the
column name tag. Position the guard column in front (before the inlet) of the analytical column.
(2) Make sure to insert the tubing all the way to the end fitting and secure it with the male nut. It is
important that there is no extra space between the tubing and the column side of the end fitting.
Presence of an extra space will allow the sample to spread out and result in wide peaks.
(3) Set the initial flow rate at 0.3 mL/min and start the system. If using the column at an elevated
temperature, keep a low flow rate untilthe temperature of the column reaches to the set temperature,
and then gradually increase the flow rate to the desired value.
Caution! • Verify that there is no solvent leak. It may cause electronic leakage, rust, and/or
chemical injury.
Attention! • Make sure not to let air bubbles enter the column while installing the column. The
air bubbles may damage the column.
• When restarting the system after column installation or after holding the eluent
flow, start the system at half of the recommended flow rate. A rapid increase in
pressure can damage the column.
• If the column was used at an elevated temperature, lower the flow rate to less than
half of the recommended flow rate at the end of analysis. Then, turn off the column
oven, and let the column temperature return to room temperature before stopping
the pump. If the pump was stopped while the eluent inside the column was still hot,
as the eluent temperature decreases, its volume also decreases. This results in
creating an empty space in the column and may deteriorate the column.
5. Column Specifications
Product Code
Product Name
Column Size (I.D.
x Length) (mm)
Particle Size
(µm)
Pore Size
(Å)
Theoretical Plate
Number (Per Column)
F7001012
RSpak DS-413
4.6 x 150
3.5
200
≥ 11,000
F6700140
RSpak DS-G
4.6 x 10
10
-
(Guard Column)
Base Material: Spherical porous particles of styrene divinylbenzene copolymer
Column Housing: SUS-316 (stainless steel)
Screw Type: Internally-threaded type No. 10-32 UNF
Shipping Solvent: Water/Acetonitrile/THF=40/30/30

Operation Manual Shodex RSpak DS-413 Version 191023E
4
6. Usable Conditions
Product Name
Flow Rate (mL/min)
Maximum Pressure
(MPa)
pH Range
Temperature
Range (°C)
Recommended
Maximum
RSpak DS-413
0.6 - 1.0
1.0
20
1 to 13*1
20 to 50*2
RSpak DS-G
-
-
-
2 to 12*1
*1: Do not use hydrochloric acid as it corrodes stainless steel.
*2: When THF is not contained in eluent, the maximum usable temperature increases to 70°C.
Attention! • Use the column within above stated flow rate, pressure, and temperature ranges.
Using the column outside the given range may damage the column and lower its
performance.
7. Eluents
(1) The eluent cannot be 100 % water. Be sure to mix with organic solvent. Mix 30 % or more for
methanol. 10 % or more for acetonitrile and 5 % or more for THF. The mentioned organic solvents can
be used up to 100 %.
(2) Buffer bases such as phosphate, acetate, tris-HCI and salts such as sodium chloride, potassium
chloride, sodium sulfate can be added up to 0.5 M. However, when adding chloride ion, please use at
pH 4.0 or above.
(3) Degas the eluent fully to prevent the formation of air bubbles.
(4) Presence of small debris or insoluble substances may result in deterioration of the column and/or
they appear as noise on the chromatograms. Filter the eluent with a 0.45 µm disposable filter to
prevent the problems.
(5) Check miscibility/solubility of the desired new eluent and eluent previously used in the system. If
the new eluent has low miscibility/solubility to the eluent previously used, first use the eluent that is
miscible/soluble to both eluents, and then replace it with the desired eluent.
(Ex.) When replacing buffer solution (or salt solution) to acetonitrile/water, first run 100 %
pure water and then replace it with the eluent.
(6) Start the system at less than 0.3 mL/min when replacing the solvent.
(7) Use of viscous solvents will increase the column pressure. Make sure to adjust the flow rate and/or
column temperature so that thecolumn pressure remains below the maximum column pressure stated
in section "6. Usable Conditions".
(8) As the eluent composition changes during the gradient elution, it may increase the column pressure
to above the column's maximum pressure. Make sure to adjust the flow rate and/or column
temperature so that the column pressure remains below the maximum column pressure.
Attention! • Always use freshly prepared solvents. Solvents stored for a long time may have
changed their compositions and may influence elution patterns and/or damage the
column.
• Frequent solvent replacement can deteriorate the column, and thus not
recommended.
• When using highly corrosive salts such as sodium chloride, wash out the salts after
the analysis. The metal parts of the devices and/or the columns may rust.
Note • Use of on-line degasser is recommended.
• It is recommended to set the pump limiter to avoid use exceeding the maximum
pressure.

Operation Manual Shodex RSpak DS-413 Version 191023E
5
8. Sample Preparation
(1) If possible, use the eluent for analysis to dissolve or dilute samples. If this is difficult, use a solvent
which has a composition that is as close as possible to the eluent's composition, but which fully
dissolves or dilutes the sample. When gradient elution is used, it is recommended to use the initial
eluent to prepare the sample.
(2) Filter the sample solution using disposable 0.45 μm filter to prevent the column from clogging or
deteriorating.
(3) Recommended sample injection volume is less than 20 μL per column.
Note •Use of guard column is recommended to protect the analytical column.
9. Column Cleaning
Problems in peak shapes and elution time changes or elevated column pressure etc. are often caused
by insoluble or adsorbing components present in the eluent and reagents being deposited inside the
column. These problems may be resolved by cleaning the column.
If a guard column is used with the analytical column, first remove the guard column and check the
performance of the analytical column alone. If the problem is solved, most likely the cause is from the
guard column. In this case, clean the guard column.
If the problem is not solved by removing the guard column, clean both guard and analytical columns.
Make sure to clean the guard and the analytical columns separately. When washing the column, let
the washing solution flow from the column outlet go directly into the waste container and not let the
solution go through the detector.
(1) Insoluble components that block the column inlet may be removed by reversing the flow direction,
i.e., introducing the eluent from the column outlet, with flow rate at less than half of the recommended
flow rate.
(2) Follow below cleaning steps for adsorbing components. For an efficient cleaning, reverse the
direction and waste the washing solution directly from the column outlet into the waste container
without the detector and reduce the flow rate at less than 0.3 mL/min. A suggested cleaning solvent
volume is 5 - 10 times the column volume. Make sure to clean the guard and the analytical columns
separately.
(Ex. 1: Alkaline washing) -When the analysis condition is under acidic to neutral conditions-
Flow 10 mM sodium hydroxide aq./acetonitrile=80/20 through the column as cleaning solvent.
(Ex. 2: Acid washing) -When the analysis condition is under alkaline to neutral conditions-
Flow 50 mM phosphoric acid aq./acetonitrile=90/10 through the column as cleaning solvent.
(Ex. 3: Organic solvent washing) -When the analysis condition is under conditions with high water ratio-
Flow water/acetonitrile=20/80 through the column as cleaning solvent.
Attention! • Presence of acidic or alkaline solvent for a long time may accelerate deterioration of
the column. Do not stop in the middle of column washing until the replacement of the
in-column solvent with the eluent is complete.
• A strongly alkaline solvent such as aqueous sodium hydroxide may damage the
detector cell. Do not connect the detector and be sure to connect the cleaning solution
directly from the column outlet to the waste container.

Operation Manual Shodex RSpak DS-413 Version 191023E
6
10. Column Storage
Remove the column from the system after replacing the in-column solvent with water. Securely tighten
the end caps and store the column at a location with stable temperature (a cool and dark space is
recommended). Refer to section “7. Eluents”when replacing the eluent.
Attention! • Never allow inside of the column to dry. It can damage the column.
11. Column Inspection
Inspection method is described in the Certificate of Analysis (CoA). Theoretical Plate Number (N) and
Asymmetry Factor (Fas) were calculated using the below equations.
(1) Theoretical Plate Number (N)
(2) Asymmetry Factor (Fas)
12. Additional Warnings
(1) Do not remove end fittings.
(2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface.
Refer to the Shodex website (www.shodex.de) for product details and their applications. Contact
through the Shodex website or Shodex partners for additional assistance.

Operation Manual Shodex RSpak DS-413 Version 191023E
7
13. Warranty (Version 4)
(1) Showa Denko K.K. warrants that the Shodex™product, at the time of delivery to the user, will
conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used
in accordance with the attached operating manual. The foregoing warranty is exclusive and is in lieu
of all other warranties with respect to the Shodex product, whether written, oral, implied, statutory or
otherwise. No warranties by Showa Denko K.K. are implied or otherwise created, including, but not
limited to, the warranty of merchantability and fitness for particular purposes.
(2) Any claim of inconformity to the specification must be notified to Showa Denko K.K. within ten (10)
days after delivery to the user. User’s exclusive remedy and Showa Denko K.K.‘s exclusive liability for
such claim are limited to the replacement of the Shodex product in question. In no event is Showa
Denko K.K. liable for any indirect, incidental or consequential damage arising out of in connection with
the Shodex Column, whether or not such damage is allegedly based on breach of warranty, negligence
or otherwise.
(3) No warranty is made in any of the following cases:
3-1 If the Shodex product is not used in accordance with the operating manual
3-2 If the Shodex product is remodelled by anyone other than person or firm designated by Showa
Denko K.K.
3-3 If the Shodex product is disposed of
3-4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K.K.
3-5 If the performance of the Shodex product is not conform to the specification of the attached
CERTIFICATE OF ANALYSIS due to any of the reasons below:
a. Computer virus
b. Impurities contained in the sample, reagent, gas air or cooling water provided by the user
c. Breakdown or malfunction of equipment, apparatus or component used in combination with the
Shodex product
d. Force majeure such as fire, earthquake, flood, other natural disaster, crime, riot, act of terrorism,
war or radioactive contamination
(4) In no event is Showa Denko K.K. liable for
4-1 the results of analyses or preparations using the Shodex product or any portion of the same,
including, but not limited to, the reliability, accuracy, efficacy and safety of said results, and
4-2 the occupational hazard in the use of the Shodex product, whether or not such use is made in
accordance with the attached Conditions for use.
(5) The Shodex Column is for laboratory use only. It must not be used for clinical diagnosis. Showa
Denko K.K. is not liable for any use of the Shodex product except laboratory use.