Integra PIPETBOY acu 2 User manual

PIPETBOY acu 2 Operating instructions
466079_V13

Declaration of conformity
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the devices
comply with:
EU Regulations
Standards for EU
Standards for Canada and USA
Zizers, November 4, 2016
Description Models
PIPETBOY acu 2 155000, 155015, 155016, 155017, 155018, 155019
PIPETGIRL 155021
EU Directives (DoW: Date of Withdrawal) Before DoW DoW After DoW
Low Voltage Equipment 2006/95/EC 20.04.2016 2014/35/EU
Electromagnetic Compatibility 2004/108/EC 20.04.2016 2014/30/EU
Restriction of Hazardous Substances 2011/65/EU
Waste Electrical and Electronic Equipment 2012/19/EU
Battery Directive 2006/66/EC
Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) 1907/2006
Capacity Labelling of Portable Secondary Batteries 1103/2010
Ecodesign - Power supplies 278/2009
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
EN 61010-1: 2010
Electrical equipment for measurement, control and laboratory use -
EMC requirements.
EN 61326-1: 2013
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
CAN/CSA-C22.2
No. 61010-1
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
UL 61010-1
Operation is subject to the following two conditions: (1) this device may not
cause harmful interference, and (2) this device must accept any interfer-
ence received, including interference that may cause undesired operation.
Part 15 of the
FCC Rules
Class A
Elmar Morscher
CEO
Thomas Neher
Quality Manager

PIPETBOY acu 2 - Operating instructions V13
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Table of Contents
1 Introduction ................................................................................................................ 4
2 Description of the device............................................................................................ 5
3 Installation .................................................................................................................. 6
4 Operation ................................................................................................................... 7
5 Maintenance............................................................................................................. 10
6 Technical Data ......................................................................................................... 11
7 Accessories.............................................................................................................. 12
Imprint
© 2018 INTEGRA Biosciences AG
This operating instruction manual applies to serial number 1350 000 or higher of
PIPETBOY acu 2 / PIPETGIRL.
Manufacturer
Customer service
Please contact your local INTEGRA Biosciences representative, see
www.integra-biosciences.com or contact [email protected].
INTEGRA Biosciences AG
CH-7205 Zizers, Switzerland
T [phone]
F [phone]
INTEGRA Biosciences Corp.
Hudson, NH 03051, USA
T [phone]
F [phone]
[e-mail]
www.integra-biosciences.com

PIPETBOY acu 2 - Operating instructions V13 Introduction
4 INTEGRA Biosciences AG
1 Introduction
1.1 Intended use
PIPETBOY acu 2 is a pipette controller designed for aspirating and dispensing aqueous
solutions with plastic or glass pipettes of 1 to 100 ml volumes. It is intended for
measurement, control and laboratory use. Any use of this instrument in a medical or IVD
setting is under the sole responsibility of the user.
PIPETGIRL is a special model of PIPETBOY acu 2 and provides the same functions as
PIPETBOY acu 2.
1.2 Safety notes
1) Do not use or charge PIPETBOY acu 2 in an atmosphere with danger of explosion.
Also, do not pipette highly flammable liquids such as acetone or ether.
2) When handling dangerous substances, comply with the material safety data sheet
(MSDS) and with all safety guidelines such as the use of protective clothing and safety
goggles. Never point a pipette in anyone’s direction.
3) Avoid pipetting of liquids whose vapours could attack the materials PA (polyamide),
POM (polyoxymethylene), FPM (fluor-rubber), NBR (nitrile-rubber), CR (chloroprene),
silicone. Corrosive vapours could also damage metallic parts inside the device.
4) Use an original INTEGRA Biosciences mains adapter only and protect it from moisture,
otherwise PIPETBOY acu 2 might be damaged.
5) Prolonged exposure of PIPETBOY acu 2 to UV-light can cause discolouration and/or
yellowing of the plastic housing. However, this will not affect the performance of the
device in any way.
6) Old Li-ion batteries may cause a safety risk. We recommend to replace the battery after
3 years of use. Also replace the battery if the charging intervals are unusually short or
if the charging takes much longer than usual (4 hours or more). These are indicators
that the battery has reached the end of its life-cycle.
7) Li-ion technology bears the risk of thermal runaway and cell rupture if the battery was
damaged. Do not expose the battery to heat (> 60°C) and avoid mechanical stress.
Batteries which were subject to deep discharges may develop internal short circuits,
leading to an increased self-discharge rate and heating during battery charging. This
may also result in thermal runaway and cell rupture.
8) To extend the battery life-cycle, it is recommended to charge the battery every 2
months if the pipette controller is not used regularly. If the pipette controller is not used
for more than 6 months, remove the battery from the instrument.
Regardless of the listed safety notes, additionally applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com on a regular basis for up to date
information regarding REACH classified chemicals contained in our products.

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2 Description of the device
2.1 Scope of delivery
• PIPETBOY acu 2 / PIPETGIRL device
• 1 rechargeable Li-ion battery
• Mains adapter
• Wall mount
• Hydrophobic sterile filter 0.45 µm (spare)
• Quick Start Guide
2.2 Overview of PIPETBOY acu 2
1 Aspiration button
2 Dispensing button
3 Thumb wheel to set maximum speed
4 Battery charge indicator
5 Socket for mains adapter cable
6 Handle
7 Sterile module complete (=nose piece)
7 Sterile module complete (=nose piece)
7a O-ring
7b Housing of sterile module, upper part
7c Filter rubber
7d Hydrophobic filter
7e Pipette mount
7f Housing of sterile module, lower part
1
2
4
5
7
6
3
c
d
e
f
b
7a

PIPETBOY acu 2 - Operating instructions V13 Installation
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3 Installation
3.1 Charging the battery
A full charge takes 3.5 hours. Before the first use, PIPETBOY acu 2 should be charged
until the battery charge indicator (4) turns green, showing that the battery is full.
When the battery charge indicator (4) starts flashing red, PIPETBOY acu 2 can be used
for around 100 pipetting cycles before shutting down. It should thus be recharged
immediately.
PIPETBOY acu 2 has an integrated protection: it will not overcharge even if it is connected
to power for indefinite time. To avoid unnecessary power consumption, it is recommended
to unplug the power supply when the charge indicator is green. PIPETBOY acu 2 can be
used while it is being charged.
The battery charge indicator provides various information:
3.2 Replacing the battery
3.3 Mounting of the wall mount
The wall mount serves to park PIPETBOY acu 2.
To mount the wall mount, remove the protective foil from the adhesive tape at the back of
the holder. Hold it with the sign <up> facing upwards and press it to the desired place.
Make sure that the surface onto which the wall mount is mounted is smooth, clean and
grease-free. Wait 24 hours before using the wall mount for the first time. Alternatively the
wall mount can be fixed with the included screws.
Battery charge indicator Battery status and information
Flashes red Battery is low. Charging is needed.
Is red and power supply is connected Battery is being charged.
Is green and power supply is connected Battery is fully charged.
Flashes alternately red and green Battery error. Check if the correct type of
battery with correct polarity (+/-) and/or
power supply are used.
1) Move the lid of the battery compartment upwards and
remove it (a).
2) Replace the old battery with an original
INTEGRA Biosciences rechargeable battery (see “7
Accessories” on page 12) and make sure that it is
inserted with the correct polarity (+/-).
3) Close the battery compartment with the lid (a).
a

PIPETBOY acu 2 - Operating instructions V13 Operation
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4 Operation
4.1 Inserting the pipette
WARNING
Do not insert pipettes with force into PIPETBOY acu 2, because they can break
and cause injury, particularly thin pipettes made of glass.
4.2 Pipetting
Press the aspiration button (1) to fill the pipette and the dispensing button (2) to empty it.
The aspiration and dispensing speed can be controlled in two manners:
• Fine speed adjustment by varying the finger pressure on the buttons (1, 2).
• Step-less presetting of the maximum pump speed by turning the thumb wheel (3) to
optimally match the pipette volume (turning to the left = slower pump speed, for small
pipettes; to the right = faster, for large pipettes).
To empty the pipette by gravity force, press the dispensing button only slightly in order to
avoid reaching the trigger point where the pump starts running. Gravity dispensing is used
for “to deliver” (TD) pipettes that are not of the “blow-out” type (blow-out pipettes have two
thin rings or a frosted band around the neck).
PIPETBOY acu 2 is featured with a “TURBO” mode. Plug the mains adapter cable into the
pipette controller and turn the thumb wheel completely to the right for maximal speed.
The silicone pipette mount (7e) has a special conical
channel to guarantee a firm and leak-proof grip of the
pipette independently of its diameter.
Disassemble the nose piece (see “5.1 Cleaning and
servicing” on page 10) and orient the pipette mount:
a) with the large opening facing down for pipettes > 2 ml
(factory setting), or
b) with the small opening facing down for pipettes < 2 ml.
a) b)

PIPETBOY acu 2 – Operating instructions V13 Operation
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4.3 Troubleshooting Problem Probable cause Remedy Pipette drips (leak in the system).
Pipette is damaged or not fully inserted in the nose piece (7).
Reinsert a new pipette and push it all the way into the nose piece. Make sure that the pipette mount orientation is correct (see section 4.1).
The inside of the pipette mount (7e) is
damaged resulting in insufficient sealing of the pipette neck.
Replace the pipette mount (#151 020).
The filter rubber (7c) or the filter (7d) in the
nose piece are damaged or missing causing a leak.
Replace the filter rubber (#153225) and/or the filter (Part No. see “7 Accessories” on page 12).
Reduced aspiration efficiency or no liquid aspiration.
The filter (7d) is wet or dirty. Replace filter.
The nose piece (7) is not tight. Tighten the nose piece, or replace defective parts.
The battery is discharged (battery charge indicator flashes red)
Charge the battery.
The battery is missing. Insert the battery or connect the instrument to the
mains adapter.
The battery is defective. Replace the battery.
The battery is wrongly inserted. Insert correctly, note polarity (+) and (-).
Reduced operating time with fully charged battery.
The battery is worn. Replace the battery. Wrong battery type is inserted. Use only original Li-ion battery (#155 066).
Extremely long charging time of battery.
Wrong mains adapter is used. Use only original mains adapter (see “7
Accessories” on page 12).
Extremely short charging and operating time.
Wrong battery type is inserted. Use only original Li-ion battery (#155066).

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Battery is not charging. The battery is wrongly inserted. Insert correctly, note polarity (+) and (-).
Wrong battery type is inserted. Use only original Li-ion battery (#155066). Wrong mains adapter is used. Use only original mains adapter (see section 7).
Battery operation not possible.
The battery is wrongly inserted. Insert correctly, note polarity (+) and (-). Wrong battery type is inserted. Use only original Li-ion battery (#155066). The battery is missing. Insert the battery.
Problem Probable cause Remedy

PIPETBOY acu 2 - Operating instructions V13 Maintenance
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5 Maintenance
After maintenance work, perform a leak test to ascertain correct functioning of
PIPETBOY acu 2: liquid should not leak out of a filled pipette before the dispensing button
is pressed.
5.1 Cleaning and servicing
PIPETBOY acu 2 can be cleaned with a cloth moistened with soapy water or with a 70 %
ethanol.
Disassembly of the nose piece:
It is recommended to change the hydrophobic filter (7d) every three months. Should the
filter get wetted or soiled, it has to be changed immediately. The filter must be oriented
with the blue (0.45 µl) / red (0.2 µl) side facing upwards towards PIPETBOY acu 2.
5.2 Decontamination
The nose piece housing (7b, 7f), the pipette mount (7e) and the filter rubber (7c) can be
autoclaved at 121 °C, 1 bar overpressure for 20 minutes. Silicone may become brittle after
extensive autoclaving. Replace the pipette mount and filter rubber if they are damaged.
If the housing of the PIPETBOY acu 2 have been in contact with biohazardous material,
it must be decontaminated in accordance to good laboratory practice. Do not spray directly
on the instrument but use a lint-free cloth, lightly soaked with a disinfectant and wipe dry
directly after decontamination. Never use acetone or other solvents! Follow the
instructions provided by the disinfectant manufacturer.
5.3 Equipment disposal
PIPETBOY acu 2 device must not be disposed of with unsorted municipal
waste. Do not dispose of the device in a fire.
PIPETBOY acu 2 contains a Li-ion battery. Do not modify the battery in any
way. Dispose of the PIPETBOY acu 2 device and the battery separately in
accordance with the laws and regulations in your area governing disposal of
devices containing Li-ion batteries.
Unscrew the nose piece (7) from the handle by turning it
counter clockwise. Hold the upper part of nose piece (7b),
press the lower part (7f) firmly against the upper part (7b)
and turn it counter clockwise (left). The lower part of nose
piece housing (7f) will disengage after about 1/8th of a
turn.
Remove the pipette mount (7e), the filter (7d) and the
filter rubber (7c), if required.

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6 Technical Data
6.1 Specifications
6.2 Chemical compatibility
The table below lists PIPETBOY acu 2 parts that come into contact with the aspirated
liquid or its aerosols and vapors, and rates the compatibility of these parts to a few of the
chemicals commonly used in laboratories. To determine the compatibility of a component
to a chemical not listed in the table, please consult one of the several tables available on
the internet. Note that the rating refers to soaking in the concentrated chemical; however,
more relevant here is the attenuated effect resulting from vapors and the diluted chemical.
It is recommended to test the compatibility of relevant components to a specific chemical
prior to extensive use.
INTEGRA Biosciences does not warrant that the information in the table is accurate or
complete and that any material is suitable for any purpose.
Pipetting speed max. 13.5 ml/s (with a 50 ml serological pipette)
Battery Type: rechargeable, Li-ion, min. 500 mAh
Typical charging time: 3.5 hours
Charging cycles: 500–1000 (when charging as indicated)
Running time: at least 5500 cycles of aspiration and
dispensing of 25 ml.
Electricity supply Mains adapter input: 100–240 VAC, 50/60 Hz
Device Input: 16–19 VDC, 3.1 W
Materials Housing: PA
Nose piece housing: POM
Pipette mount: Silicone
Filter rubber: Silicone
Dimensions (H x W x D) 125 x 130 x 35 mm
Weight 195 g
Ambient conditions Operation: 5–40°C, max. 80% RH
Storage: -10–50°C, max. 95% RH

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12 INTEGRA Biosciences AG
Chemical compatibility chart
Compatibility ratings:
A = Good: no or minor effects.
B = Fair: moderate effects, not recommended for continuous use.
C = Critical: not recommended, suitability to be determined by test.
7 Accessories
Parts Materials
JAVEL (e.g. NaClO)
Acetic acid
Ethanol
Isopropyl alcohol
NaCl saturated
Sodium hydroxide (50%)
Sodium acetate
(3M, pH 5.2)
Hydrochloric acid (20%)
Chloroform
Acetone
Handle PA A C B A A A A C C A
Nose piece housing POM C C A A A A A B A A
Pipette mount,
Filter rubber, tubings
Silicone A B A A A A C A C C
Internal parts
(e. g. pump)
FPM AAAAAC C AAC
NBR ABBAAA A ACC
CR AAAAAA A ABA
Metal CCAABC A CAA
Accessories Part No.
Stand for
PIPETBOY
for a well organized work area, to park PIPETBOY acu 2 with
inserted pipette
155065
Wall mount for holding PIPETBOY acu 2 on the wall 155521
Mains adapter
(100-240 VAC,
50/60 Hz)
EU version 153210
US/JP version 153211
UK version 153214
AU version 153216

PIPETBOY acu 2 - Operating instructions V13 Accessories
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WARNING
Consumables Part No.
Sterile module
(nose piece)
complete (7)
turquoise for PIPETBOY acu 2 classic and transparent;
with filter 0.45 µm, unsterile
155025
classic (grey) for PIPETBOY acu 2 green, red, blue, purple
and PIPETGIRL; with filter 0.45 µm, unsterile
155070
Sterile module
(nose piece)
housing (7b,7f)
turquoise, for PIPETBOY acu 2 classic and transparent 155230
classic (grey), for PIPETBOY acu 2 green, blue, red, purple
and PIPETGIRL
155060
Filter 0.45 µm
(7d)
sterile 153015
unsterile 153016
Filter 0.2 µm
(7d)
sterile 156608
unsterile 156607
Pipette mount
(7e)
silicone, for holding pipette in the sterile module (nose piece) 151020
Filter rubber (7c) silicone, for holding the filter in the sterile module (nose
piece)
153225
O-ring (7a) 153235
Battery
compartment lid
classic (grey) 155203
green 155160
red 155161
blue 155162
transparent 155163
purple 155164
pink (PIPETGIRL) 155166
Battery Li-ion for PIPETBOY acu 2 / PIPETGIRL
(with serial number >=1350001)
155066
Only use the battery Li-ion for PIPETBOY acu 2
(serial number >=1350001; LED is white when inactive,
red or green when active)!
The NiMH battery for PIPETBOY acu is not compatible!
serial number