Leica VT1000 S User manual

Instruction Manual
Leica VT1000S V1.6 English - 11/2007
Always keep this manual near the instrument.
Read carefully prior to operating the instrument.
Leica VT1000 S
Vibrating-blade
microtome

3
Leica VT1000 S – Microtome
Issued by :
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Telephone: [phone]
Facsimile: [phone]
eMail: [email protected]
Internet: http://www.histo-solutions.com
These are determined only by the contract provi-
sions agreed between ourselves and our custom-
ers.
Leica reserves the right to change technical speci-
fications as well as manufacturing processes with-
out prior notice. Only in this way is it possible to
continuously improve the technology and manufac-
turing techniques used in our products.
This document is protected under copyright laws.
Any copyrights of this document are retained by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any
parts thereof) by means of print, photocopy, micro-
fiche, web cam or other methods – including any
electronic systems and media – requires express
prior permission in writing by Leica Biosystems
Nussloch GmbH.
For the instrument serial number and year of manu-
facture, please refer to the name plate at the back
of the instrument.
© Leica Biosystems Nussloch GmbH
The information, numerical data, notes and value
judgments contained in this manual represent the
current state of scientific knowledge and state-of-
the-art technology as we understand it following
thorough investigation in this field. We are under no
obligation to update the present manual periodically
and on an ongoing basis according to the latest
technical developments, nor to provide our custom-
ers with additional copies, updates etc. of this
manual.
For erroneous statements, drawings, technical il-
lustrations etc. contained in this manual we exclude
liability as far as permissible according to the na-
tional legal system applicable in each individual
case. In particular, no liability whatsoever is ac-
cepted for any financial loss or consequential dam-
age caused by or related to compliance with state-
ments or other information in this manual.
Statements, drawings, illustrations and other infor-
mation as regards contents or technical details of
the present manual are not to be considered as
warranted characteristics of our products.
Note

4Instruction Manual V 1.6 – 11/2007
Table of contents
1. Important notes .............................................................................................................. 5
1.1 Symbols used in this manual and their meaning ...................................................... 5
1.2 Qualification of personnel .............................................................................................. 5
1.3 Designated use / improper use ..................................................................................... 5
2. Safety ................................................................................................................................6
2.1 General safety instructions ............................................................................................ 6
2.2 Safety instructions for handling the instrument ......................................................... 7
3. Instrument properties ................................................................................................... 8
3.1 Technical Data ................................................................................................................. 8
3.2 Overview - VT1000S ........................................................................................................ 9
4. Installation .................................................................................................................... 11
4.1 Standard delivery ........................................................................................................... 11
4.2 Unpacking and installing the instrument .................................................................. 12
5. Operation........................................................................................................................ 13
5.1 Setting up the instrument ............................................................................................. 13
5.2 The VT 1000 S controls and their function ................................................................ 14
5.3 Adjusting the amplitude ............................................................................................... 18
5.4 Working with the VT 1000 S on a daily base ............................................................ 19
5.5 Daily routine maintenance / switching the VT1000S off......................................... 22
6. Trouble shooting .......................................................................................................... 23
7. Cleaning and maintenance ....................................................................................... 28
7.1 Cleaning the instrument ............................................................................................... 28
7.2 Exchanging the main fuses .......................................................................................... 29
8. Ordering information: replacement parts, accessories and consumables .. 30
8.1 Additional accessories for standard size specimens (functional description) .. 31
8.2 Additional accessories for large specimens (functional description) ................ 31
8.3 Foot switch (functional description) ........................................................................... 32
8.4 Magnifier, fiber optics, cold light source .................................................................. 33
9. Warranty and service ................................................................................................. 34
10. EC Declaration of Conformity .................................................................................... 35
11. Peoples Republic of China......................................................................................... 36

5
Leica VT1000 S – Microtome
1.1 Symbols used in this manual and
their meaning
Warnings
appear in a grey box and are marked
by a warning triangle:
Useful notes,
i.e. important user information ap-
pear in a grey box and are marked by
an information symbol:
Figures in brackets refer to item
numbers in drawings or to the draw-
ings themselves.
1. Important notes
(5)
(Fig.5)
Instrument type:
All information given in this instruction manual
applies only to the VT1000 S.
A name plate, indicating the instrument serial
number, is attached to the back of the instru-
ment.
Required information for all inquiries:
For any inquiries please specify:
• Instrument type
• Serial number
1.2 Qualification of personnel
The Leica VT1000S should be operated by
trained laboratory personnel only.
Prior to starting work with the instrument, all
laboratory personnel designated to operate the
instrument must carefully read the present in-
struction manual and must be familiar with all
technical features of the instrument.
1.3 Designated use / improper use
The VT1000S has been designed for section-
ing of specimens in medicine, biology and in-
dustry, especially for sectioning fixed or fresh
tissue immersed in buffer solution.
The instrument must be operated exclusively
according to the instructions contained in this
manual.
Any other use of the instrument is considered
improper.

6Instruction Manual V 1.6 – 11/2007
This instruction manual includes important instructions and information
regarding the operating safety and maintenance of the instrument.
The instruction manual is an important part of the product. It must be read
carefully before using the instrument for the first time and must always be
kept with the instrument.
If additional requirements, which exceed the scope of this manual, are imposed
by regulations and/or laws on accident prevention and environmental
protection in the country of operation, appropriate instructions for compliance
with such requirements must be added to this manual.
Read this instruction manual carefully before attempting to work on or operate
the instrument.
2. Safety
2.1 General safety instructions
These instruments have been built and tested in accordance with the
safety regulations for electrical measuring, control, regulating and labo-
ratory devices.
In order to maintain this condition and to ensure safe operation, the user
must follow the instructions and warnings contained in this Operating
Manual.
For current information about applicable standards, please refer to the
CE Declaration of Conformity on our Internet site:
www.histo-solutions.com

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Leica VT1000 S – Microtome
Caution / danger
Caution: risk of injury when touching
the knives and blades as these are
extremely sharp.
Warning: risk of infection when
working with fresh tissue or with
material where an infection cannot
be excluded.
Caution: When not in use, cover
magnifier with corresponding lid to
avoid risk of fire.
Warning: Avoid touching live parts
under any circumstances!
2. Safety
2.2 Safety instructions for handling the instrument
Correct behavior
Be sure to handle knives and blades very cau-
tiously!
Never touch the cutting edge of knives and
blades!
Do not leave knives, blades and bladed knife
holders unprotected.
Take adequate protective measures to elimi-
nate risk of infection!
Protective clothes according to safety regula-
tions "Working with harmful substances"
(Safety mask, gloves, protective clothing) must
be worn!
The magnifier must be covered while the in-
strument is not in use, as it may act as a burn-
ing glass when not covered!
In case of emergency, press the red EMER-
GENCY STOP switch (at the right side of the in-
strument). To release the switch, turn it in the
direction of the arrow.
The instrument cover may only be removed by
qualified service personnel!
Before removing the cover, ensure that the in-
strument is unplugged.

8Instruction Manual V 1.6 – 11/2007
3. Instrument properties
3.1 Technical Data
General:
Sectioning frequency (± 10 %) ......................................................................................................... 0 - 100 Hz
Amplitude .................................................................................. adjustable in 5 steps: 0.2; 0.4; 0.6; 0.8; 1 mm
Sectioning speed (± 10 %) ...................................................................................................... 0.025 - 2.5 mm/s
Return stroke speed (± 10 %)................................................................................................................ 5 mm/s
Total vertical specimen stroke ......................................................................................... 15 mm (motorized)
Sectioning range .......................................................................................................... 1 - 40 mm (adjustable)
Specimen retraction .............................................................. 0 - 999 μm (adjustable; can be deactivated)
Maximum specimen size:
with standard knife holder ........................................................................................................ 33 x 40 mm
with knife holder L ...................................................................................................................... 70 x 40 mm
Specimen orientation.................................................................................................................................. 330°
Section thickness selection .................................................................................. 1 - 999 μm, in 1-μm steps
Magnifier, assy. (Standard accessory) ...................................................................................................... 2 x
Ambiance conditions:
Working temperature range .................................................................................... min. 10 °C to max. 40 °C
Relative humidity of air ..................................................................................................................... max. 60 %
Altitude (site): ....................................................................................................max. 2,000 m above sea level
Electrical specifications:
Nominal voltage range (± 10 %): ................................................................................................100 V - 240 V
Rated frequency (± 10 %): ................................................................................................................ 50 - 60 Hz
Power draw ................................................................................................................................................ 35 VA
Mains fuse............................................................................................................................................... T 1.25 A
Pollution degree ................................................................................................................................................. 2
Overvoltage installation category ................................................................................................................. II
Overload protection ...................................................................................................................................... yes
Internal current limiter of electronics ....................................................................................................... yes
Dimensions:
L x W x H ................................................................................................................ 480 mm x 360 mm x 200 mm
Height with magnifier............................................................................................................................. 285 mm
Weight:
(without magnifier) ..................................................................................................................................... 17 kg
(magnifier only) ............................................................................................................................................. 2 kg
(total) ............................................................................................................................................................. 19 kg

9
Leica VT1000 S – Microtome
3. Instrument properties
3.2 Overview - VT1000 S
Buffer tray S
Emergency stop switch
(not visible here)
Fixture
for magnifier
Fiber optics (optional)
Magnifierglass
Cold light source
Leica CLS 100 (optional)
Knife holder
Control panel
Magnifier
carrier
Cutting head

10 Instruction Manual V 1.6 – 11/2007
3. Instrument properties
Control knob for sectioning
frequency
LED mode indication:
'Totalized section thickness’
'Section thickness’
CLR-Clear button
DISP-Programming button
Selector button
'Single/ Continuous stroke'
(LEDs indicate selected mode)
Toggle switch for knife
forward and return stroke
Toggle switch
'Buffer tray height adjustment
(LEDs indicate limit positions)
Pause button -
stops sectioning process
Control knob
for sectioning speed
Indication of selected section
thickness or totalized section
thickness in μm
+/- Selection button
for sectioning thickness
(1 - 999 μm selectable),
retraction and/or volume
Button for setting the limit
stops of the sectioning
window
Start button
for single / continuous
sectioning stroke
Emergency stop button
Fixing device for drain tube
Connecting socket for power cord
Mains switch
9-Channel plug for foot
switch
Button for maximum advance
speed