Leica RM2255 User manual

Instructions for Use
Leica RM2255
Rotary Microtome
Instructions for Use
Leica RM2255 V 2.2, English 09/2016
Order No. 14 0502 80101 RevI
Always keep this manual with the instrument.
Read carefully before working with the instrument.

1
Leica RM2255
The information, numerical data, notes and value
judgments contained in this manual represent
the current state of scientific knowledge and
state-of-the-art technology as we understand it
following thorough investigation in this field.
We are under no obligation to update the present
manual periodically and on an ongoing basis ac-
cording to the latest technical developments, nor
to provide our customers with additional copies,
updates etc. of this manual.
To the extent permitted in accordance with
the national legal system as applicable in each
individual case, we shall not be held liable for
erroneous statements, drawings, technical illus-
trations etc. contained in this manual.
In particular, no liability whatsoever is accepted
for any financial loss or consequential damage
caused by or related to compliance with state-
ments or other information in this manual.
Statements, drawings, illustrations and other
information as regards contents or technical
details of the present Instructions for Use are not
to be considered as warranted characteristics of
our products.
These are determined only by the contract
provisions agreed between ourselves and our
customers.
Leica reserves the right to change technical
specifications as well as manufacturing process-
es without prior notice. Only in this way is it pos-
sible to continuously improve the technology and
manufacturing techniques used in our products.
This document is protected under copyright laws.
All copyrights to this documentation are held by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or
of any parts thereof) by means of print, photo-
copy, microfiche, web cam or other methods
– including any electronic systems and media
– requires express prior permission in writing by
Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the identification
label attached to the instrument.
© Leica Biosystems Nussloch GmbH
NOTE
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: [phone]
Fax: [phone]
Internet: http://www.LeicaBiosystems.com
Assembly contracted to Leica Microsystems Ltd. Shanghai

2Instructions for Use, V 2.2 RevI – 09/2016
1. Important Information......................................................................................................................................4
1.1 Symbols in the text and their meanings ................................................................................................... 4
1.2 Qualification of personnel........................................................................................................................... 5
1.3 Intended use of instrument......................................................................................................................... 5
1.4 Instrument type............................................................................................................................................. 5
2. Safety..................................................................................................................................................................6
2.1 Safety notes .................................................................................................................................................. 6
2.2 Warnings ....................................................................................................................................................... 6
2.3 Integrated safety devices ........................................................................................................................... 9
3. Instrument Components and Specifications.............................................................................................11
3.1 Overview — instrument components..................................................................................................... 11
3.2 Instrument specifications ......................................................................................................................... 12
3.3 Technical Data............................................................................................................................................ 13
4. Instrument Setup ............................................................................................................................................15
4.1 Installation site requirements .................................................................................................................. 15
4.2 Standard delivery ....................................................................................................................................... 15
4.3 Unpacking and installation ....................................................................................................................... 16
4.4 Assembling the handwheel ...................................................................................................................... 18
4.5 Electrical connections............................................................................................................................... 18
5. Operation.............................................................................................................................................................21
5.1 Operating elements and their functions................................................................................................. 21
5.1.1 Instrument control panel........................................................................................................................... 21
5.1.2 Control panel............................................................................................................................................... 22
5.2 Switching on the instrument .................................................................................................................... 23
5.3 Display and control elements................................................................................................................... 24
5.4 Inserting the knife holder.......................................................................................................................... 35
5.5 Inserting the universal cassette clamp .................................................................................................. 36
5.6 Adjusting the clearance angle................................................................................................................. 37
5.7 Clamping the specimen............................................................................................................................. 38
5.8 Clamping the knife / disposable blade.................................................................................................... 38
5.9 Trimming the specimen............................................................................................................................. 40
5.9.1 Trimming in manual operation ................................................................................................................. 40
5.9.2 Trimming in motorized operation............................................................................................................. 40
5.10 Sectioning.................................................................................................................................................... 41
5.11 Changing the specimen or interrupting sectioning .............................................................................. 41
6. Optional Accessories....................................................................................................................................42
6.1 Assembly for fixture for specimen clamps ............................................................................................ 42
6.1.1 Rigid fixture for specimen clamps........................................................................................................... 42
Table of Contents

3
Leica RM2255
6.1.2 Directional fixture for specimen clamps ................................................................................................ 42
6.1.3 Fine-directional fixture for specimen clamps........................................................................................ 43
6.1.4 Quick clamping system ............................................................................................................................. 44
6.2 Specimen clamps and holders................................................................................................................. 45
6.2.1 Standard specimen clamp........................................................................................................................ 45
6.2.2 Vee insert..................................................................................................................................................... 46
6.2.3 Foil clamp type 1......................................................................................................................................... 47
6.2.4 Universal cassette clamp ......................................................................................................................... 48
6.2.5 Holder for round specimens..................................................................................................................... 49
6.2.6 Super mega-cassette clamp .................................................................................................................... 50
6.3 Knife holder base and knife holder ......................................................................................................... 51
6.3.1 Knife holder base, without lateral displacement feature .................................................................... 51
6.3.2 Knife holder E/E-TC .................................................................................................................................... 52
6.3.3 Knife holder N/NZ....................................................................................................................................... 55
6.4 Blades/knives.............................................................................................................................................. 57
6.4.1 Disposable blades...................................................................................................................................... 57
6.4.2 Knife.............................................................................................................................................................. 57
6.5 Section waste tray ..................................................................................................................................... 59
6.6 Backlighting ................................................................................................................................................ 59
6.7 Tray............................................................................................................................................................... 60
6.8 Freezer pack................................................................................................................................................ 60
6.9 Universal microscope carrier ................................................................................................................. 61
6.10 Magnifying lens ......................................................................................................................................... 63
6.11 Cold light source ........................................................................................................................................ 64
6.12 Fiber-optic light guide................................................................................................................................ 64
6.13 Ordering information.................................................................................................................................. 65
7. Troubleshooting..............................................................................................................................................67
7.1 Instrument malfunctions ........................................................................................................................... 67
7.1.1 Error reports................................................................................................................................................ 67
7.1.2 Malfunctions, possible causes and troubleshooting ........................................................................... 67
7.2 Possible faults............................................................................................................................................. 69
8. Cleaning and Maintenance..........................................................................................................................71
8.1 Cleaning the instrument ............................................................................................................................ 71
8.2 Maintenance............................................................................................................................................... 73
8.2.1 Replacing fuses .......................................................................................................................................... 73
8.2.2 Maintenance instructions......................................................................................................................... 74
8.2.3 Lubricating the instrument........................................................................................................................ 75
9. Warranty and Service....................................................................................................................................76
10. Decontamination Certificate........................................................................................................................77
Table of Contents

4Instructions for Use, V 2.2 RevI – 09/2016
1. Important Information
1.1 Symbols in the text and their meanings
(5)
RUN/
STOP
Manufacturer
This product fulfills the requirements of
the Council's Directive 98/79/EC con-
cerning in vitro diagnostics (IVD) medical
devices.
In vitro diagnostics (IVD) medical device
Observe the Instructions for Use
Order No.
Serial number
The CSA test mark means that a prod-
uct has been tested and fulfills the
applicable safety and/or performance
standards, including the relevant stan-
dards defined or administered by the
American National Standards Institute
(ANSI), Underwriters Laboratories (UL),
the Canadian Standards Association
(CSA), the National Sanitation Founda-
tion International (NSF) and others.
Symbol for labeling electrical and elec-
tronic equipment in accordance with
Section 7 of the German Electrical and
Electronic Equipment Act (ElektroG).
ElektroG is the law on the bringing into
circulation, return and environmentally
compatible disposal of electrical and
electronic equipment.
Environmental protection symbol of the
China RoHS directive. The number in
the symbol indicates the "Environment-
friendly Use Period" of the product. The
symbol is used if a substance restricted
in China is used in excess of the maxi-
mum permitted limit.
Date of Manufacture
Function keys which must be pressed
on the control panel are shown in the
text in bold capital letters.
Numbers in parentheses refer to item
numbers in illustrations.
Notes,
i.e. important user information appears
in a gray box and is marked by an infor-
mation symbol .
Warnings
appear in a gray box and are marked by
a warning triangle .
It allows maximum 3 stacks layers.
The package contents are fragile and
must be handled with care.
The package must be kept in a dry en-
vironment.
Indicates the correct upright position of
the package.
Indicates the temperature range permit-
ted for transporting the package.
Minimum -29 °C, Maximum +50 °C

5
Leica RM2255
1.4 Instrument type
All information provided in these Instructions for
Use applies only to the instrument type indicated
on the title page.
A nameplate with the serial number is fastened
to the left side of the instrument (this figure is
only symbolic).
1.2 Qualification of personnel
• The Leica RM2255 may be operated by trained
laboratory personnel only.
• All laboratory personnel designated to operate
the Leica instrument must read these Instruc-
1. Important Information
Fig. 1
tions for Use carefully and must be familiar
with all technical features of the instrument
before attempting to operate it.
1.3 Intended use of instrument
The Leica RM2255 is a fully automatic, motor-
ized rotary microtome with a separate control
panel for creating thin sections of specimens of
varying hardness for use in routine and research
laboratories in the fields of biology, medicine and
industry.
It is designed for cutting both soft paraffin-em-
bedded and harder specimens, as long as they
are suitable for being cut manually or automati-
cally.
Any other use of the instrument will be consid-
ered as improper use!
Indicates the temperature range per-
mitted for storing the package.
Minimum +5 °C, Maximum +50 °C
Indicates the humidity range permit-
ted for storing and transporting the
package.
Minimum 10 % r.H., Maximum 85 % r.H
Tip-n-Tell indicator to monitor wheth-
er the shipment has been transported
and stored in upright position accord-
ing to your requirements. With a pitch
of 60° or more, the blue quartz sand
flows into the arrow-shaped indicator
window and sticks there permanently.
Improper handling of the shipment is
immediately detectable and can be
proven definitively.
In the Shockwatch system, a precision
glass tube shows shocks or impacts
that are above a specified intensity
through red coloration. Exceeding a de-
fined acceleration (g value) destroys
the surface tension of the liquid in the
interior of the tube. This causes the
indicator tube to change color.
Indicates the item can be recycled
where correct facilities exist.

6Instructions for Use, V 2.2 RevI – 09/2016
2. Safety
2.1 Safety notes
These Instructions for Use include important
information related to the operating safety and
maintenance of the instrument.
The Instructions for Use are an important part of
the product, and must be read carefully prior to
startup and use and must always be kept near
the instrument.
This instrument has been built and tested in ac-
cordance with the safety regulations for electri-
cal measuring, control, regulating and laboratory
devices.
To maintain this condition and ensure safe
operation, the user must observe all notes and
warnings contained in these Instructions for Use.
For current information about applicable stan-
dards, please refer to the CE declaration of con-
formity on our Internet site:
www.LeicaBiosystems.com
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis
for accident prevention. Operating the instrument safely is, above all, the responsibility of the
owner, as well as the designated personnel who operate, service or clean the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.
The safety and caution notes in this chapter must be observed at all times.
Be sure to read these notes even if you are already familiar with the operation and use of other
Leica products.
These Instructions for Use must be ap-
propriately supplemented as required
by the existing regulations on accident
prevention and environmental safety in
the operator‘s country.
The protective devices located on the instrument and the accessories must not be removed or
modified. Only service personnel qualified by Leica may repair the instrument and access the
instrument's internal components.

7
Leica RM2255
2. Safety
Warnings – Safety notes on the instrument itself
Warnings – Transport and installation
Personal safety precautions
Safety notes on the instrument itself, which are marked with a warning triangle, indicate that
the correct operating instructions (as defined in these Instructions for Use) must be followed
when operating or replacing the item marked.
Failure to adhere to these instructions may result in an accident, personal injury, damage to
the instrument or accessory equipment.
• Once unpacked, the instrument may be transported only in an upright position.
• Never lift the instrument by the handwheels or the cassette clamp. Always remove the sec-
tion waste tray before transporting the instrument.
• Caution! The voltage selector has been preset at the factory.
• Before connecting the instrument to the line voltage, please check that this setting complies
with the local power requirements of your laboratory.
• The mains cable inlet is closed with an adhesive tape that indicates the actual voltage set-
ting of the instrument upon arrival. Severe damage can be caused to the instrument if the
voltage selector is set to an incorrect voltage!
• When adjusting the voltage selector setting, the instrument must be disconnected from the
mains.
• The instrument must be connected to mains only with one of the supplied power cords and
only to grounded sockets. Do not interfere with the grounding function by using an extension
cord without a ground wire.
• The instrument is ready for operation only if a dummy plug or foot switch (optional) is con-
nected. If you hear a beeping sound (continuous) after switching on the instrument, check
that the control panels are connected correctly.
• The instrument may not be operated in hazardous locations.
• Extreme temperature fluctuations between storage facility and setup site as well as high
humidity may cause condensation to form. If this is the case, wait at least two hours before
switching on the instrument. Failure to comply with this may cause damage to the instrument.
• The protective devices located on the instrument/accessories must not be removed or modi-
fied.
• When working with microtomes, personal safety precautions must always be taken. It is
mandatory to wear work safety shoes, safety gloves, a mask and safety goggles.

8Instructions for Use, V 2.2 RevI – 09/2016
2. Safety
Safety instructions – working with the instrument
• Take care when handling microtome knives and disposable blades. The cutting edge is
extremely sharp and can cause serious injuries!
• Always remove the knife / blade before detaching the knife holder from the instrument.
Always put the knives back into the knife case when not in use!
• Never place a knife anywhere with the cutting edge facing upwards and never try to catch a
falling knife!
• Always clamp the specimen block BEFORE clamping the knife or blade.
• Lock the handwheel and cover the knife edge with the knife guard prior to any manipulation
of knife or specimen clamp, prior to changing the specimen block and during all work breaks!
• ALWAYS turn the handwheel clockwise; otherwise, the brake will not work properly.
• Always take appropriate safety precautions when sectioning brittle specimens! Specimens
may splinter!
• Ensure that liquids do not enter the interior of the instrument during work!
• Do not attempt to clamp, approach or orient the specimen during the retraction phase.
• If a block is oriented during retraction, the block will advance by the retraction value PLUS
the selected section thickness before the next section. This may cause damage to both speci-
men and knife!
• The handle of the handwheel must always be centered while in motorized sectioning mode.
Do not touch the handwheel while it is running – there is a danger of injury from the hand-
wheel lock.
• Prior to sectioning, check that the specimen is securely clamped in the specimen clamp –
failure to observe this poses the risk of damaging the specimen.
• Only authorized and qualified service personnel may access the internal components of the
instrument for service and repair!
• Before each cleaning, switch off the instrument, disconnect the power plug, remove the knife
holder completely and clean it separately.
• Always remove the blade before detaching the knife holder from the instrument.
• Lock the handwheel before each cleaning!
• Do not use any solvents containing acetone or xylene for cleaning!
• Ensure that liquids do not enter the interior of the instrument during cleaning!
• Do not turn the instrument on before it is completely dry!
• When using cleaners, please comply with the safety instructions of the manufacturer and the
laboratory safety regulations!
• Turn the instrument off with the power switch and disconnect the power plug before re-
placing the fuses! Only use fuses of the same specification! For fuse specifications refer to
Chapter 3.3 - "Technical data".

9
Leica RM2255
2.3 Integrated safety devices
2. Safety
3
1
4
2
5
Handwheel lock (manual operation only)
• To lock the handwheel, push the lever (5) outwards and
continue to turn the handwheel slowly until it locks exactly
in the 12 o'clock position. The LED (4) in the LOCK display
lights up.
Handwheel brake
Using the lever (3) on the right side of the microtome base
plate, the handwheel can be braked in any position.
• To lock the handwheel, pull the lever forward forcefully.
• To unlock the handwheel brake, push the locking lever (3)
back to its original position.
Emergency-stop function
The emergency-stop function is activated with the red
EMERGENCY STOP switch (1) on the upper left of the front
of the microtome. The sectioning motor stops immediately
when the EMERGENCY STOP switch is pressed. The red LED
in the E-STOP field (2) on the control panel of the instrument
lights up, indicating that the emergency stop function has
been activated.
To deactivate this function, turn the EMERGENCY STOP
switch in the direction of the arrow.
2
12
Important!
The LED (4) in the M-STOP field indicates only that the instrument cannot be started. It does not
provide any indication that the handwheel brake is active.
The lever (3) must be pulled completely forward with force so that the handwheel brake is
applied. The handwheel is securely locked only when the lever (5) is in the 12 o'clock position.
Caution!
Never operate the lever (5) during motorized section-
ing.
Fig. 2
Fig. 3

10 Instructions for Use, V 2.2 RevI – 09/2016
2. Safety
Knife guard on the knife holder
Each knife holder is equipped with a tightly
mounted knife guard (8, 9). This makes it possible
to cover completely the cutting edge in every
knife or blade position.
Knife holder N/NZ
The knife guard (8) of the knife holder N/NZ
can be easily positioned via the two handles (7)
(Fig. 5).
To cover the knife edge, push both cover strips
of the knife guard to the center.
Knife holder E
The knife guard on knife holder E consists of a
red foldaway handle. To cover the cutting edge,
fold the knife guard handle (9) upwards as illus-
trated in Fig. 6.
7
78
Centering the handle
For safety reasons, the handle of the handwheel
must always be centered while in motorized
sectioning mode.
• Activate the handwheel lock.
• To center the handwheel, lightly pull the han-
dle (6) outwards and swivel it into the center
of the handwheel (12) (Fig. 4).
• The handle will then lock automatically when
released.
9 1011
6
12 12
Fig. 4
Fig. 5
Fig. 6
For the new knife holder E, the two
clamping levers (10, 11) must always
remain in the position shown. Clamp-
ing lever for the blade (10) at the right,
clamping lever for the lateral displace-
ment (11) at the left.

11
Leica RM2255
3.1 Overview – instrument components
3. Instrument Components and Specifications
Handwheel handle
with centering
function
Leica RM2255
Knife guard on
the knife holder
Smooth-turning
Handwheel
Lever for
activating the
Handwheel
brake
Handwheel
locking
mechanism
Separate control
panel
Fig. 7
Knife holder base
Knife holder NZ
Section waste tray
Standard clamp
Emergency-stop
switch
Instrument
control panel
Clamping lever
of knife holder base
Socket for
backlighting

12 Instructions for Use, V 2.2 RevI – 09/2016
3. Instrument components and specifications
Fig. 8
Motor
protection
fuse
Heat sink
Voltage selector switch
with fuses
Main switch
Power supply
Connecting
cable for control
panel
Connecting jack
for foot switch
3.2 Instrument specifications
The Leica RM2255 is a motorized rotary micro-
tome.
• The specimen feed system with zero-backlash
and maintenance-free cross roller guides and
the stepper motor operated coarse feed sys-
tem are located in a dust-proof plastic housing.
• The instrument is equipped with a safety hand-
wheel with a handle that can be centered, as
well as a mechanical handwheel lock.
• All controls and LEDs are centralized in a
separate control panel.
All control elements are logically arranged in
functional groups and easily identifiable.
• The specimen retraction can be turned off.
In manual operation the retraction can be
adjusted. In motorized operation the retrac-
tion value varies with the selected sectioning
speed. An LED illuminates while the sample is
in retraction.
• The electric coarse feed operates at two
speeds. In the sectioning mode, the coarse
feed buttons have a STEP function.
• Three motorized sectioning modes (CONT,
SINGLE, and STEP) are available as well as
one manual sectioning mode, the rocking
mode ROCK.
In rocking mode, it is enough to move the
handwheel a short distance back and forth in
order to create a section.
Rear side of instrument
Magnet for holding
the wrench

13
Leica RM2255
3.3 Technical Data
3. Instrument Components and Specifications
General
Approvals: The instrument-specific marks are located next to the identi-
fication label.
Nominal supply voltages: 100 / 120 / 230 / 240 V AC ±10 %
Nominal frequency: 50/60 Hz
Maximum power consumption 340 VA
Protection class : I - according to IEC-1010, UL 3101, EN 61010
Power fuses 2 x T 3.15 A, UL listed
Pollution degree : 2 - according to IEC-1010, UL 3101, EN 61010
Overvoltage category II - according to IEC-1010, UL 3101, EN 61010
Maximum heat emission: 340 J/s
Operating temperature range: +10 °C to +35 °C
Temperature range during storage: +5 °C to +50 °C
Relative humidity: max. 80 %, non-condensing
Storage humidity: min. 10 % r.H., max. 85 % r.H.
Dimensions and weight
Basic instrument
Width (including handwheel): 413 mm
Width (excluding handwheel): 300 mm
Depth (including waste tray): 563 mm (small) / 618 mm (large)
Height (total): 305 mm (with tray on the hood)
Working height (knife blade): 100 mm (measured from the base plate)
Working height (knife blade): 168 mm (measured from the table)
Weight (without accessories): approx. 37 kg
Control panel
Width: 121 mm
Depth: 166 mm
Height: 50 mm
Height (in inclined position): 81 mm
Weight (net): approx. 0.660 kg

14 Instructions for Use, V 2.2 RevI – 09/2016
3. Instrument components and specifications
Microtome
Sectioning thickness setting:
Section thickness setting range:
Setting values:
0.50 - 100 µm
from 0.50 - 5.0 µm
from 5.0 - 20.0 µm
from 20.0 - 60.0 µm
from 60.0 - 100.0 µm
in 0.5 µm increments
in 1.0 µm increments
in 5.0 µm increments
in 10.0 µm increments
Trimming section thickness setting range:
Setting values:
1 - 600 µm
from 1.0 - 10.0 µm
from 10.0 - 20.0 µm
from 20.0 - 50.0 µm
from 50.0 - 100.0 µm
from 100.0 - 600.0 µm
in 1.0 µm increments,
in 2.0 µm increments,
in 5.0 µm increments,
in 10.0 µm increments,
in 50.0 µm increments.
Specimen feed: 24 mm ±1 mm, feed motion via step motor
Vertical stroke: 70 mm
Maximum sectioning area w/o retraction: 65 mm without specimen orientation
Maximum sectioning area with retraction: 60 mm
Specimen retraction:
in manual sectioning mode: 5 - 100 µm in 5 µm increments; can be turned off
in motorized sectioning mode: Varies with the sectioning speed; can be turned off
Electric coarse feed: 300 µm/s and 800 µm/s
Sectioning speed: 0 - 420 mm/s ± 10 %
Return stroke speed: approx. 120 - 420 mm/s ± 10 %
Repositioning of knife holder base
North-south: ± 24 mm
Lateral displacement function via blade
holder CE clamping top:
East-West: 3 positions or ± 23 mm
Maximum specimen size (L x H x W): 50 x 60 x 40 mm
Specimen orientation
Horizontal:
Vertical:
± 8°
± 8°

15
Leica RM2255
4. Instrument Setup
4.2 Standard delivery
4.1 Installation site requirements
Never operate the instrument in rooms with an explosion hazard.
• Stable, vibration-free laboratory bench with horizontal, even stage plate; practically vibration-
free floor.
• No other instruments nearby which might cause vibrations.
• Room temperature consistently between 10 °C and 35 °C.
• Obstruction-free access to the handwheel.
The Leica RM2255 standard delivery includes:
1 Leica RM2255 basic instrument
1 handwheel, complete .................................................................................................... 14 0502 37734
1 external control panel .................................................................................................... 14 0502 37950
1 section waste tray .......................................................................................................... 14 0502 37931
1 dummy plug ...................................................................................................................... 14 0443 30420
1 tool set - consisting of: ................................................................................................... 14 0502 37965
1 Allen key with handle, size 5 ................................................................................... 14 0194 04760
1 Allen key with handle, size 4 ................................................................................... 14 0194 04782
1 Allen key size 3 .......................................................................................................... 14 0222 04138
1 screwdriver 3x50, 186 long....................................................................................... 14 0170 11568
1 bottle (50 ml) of oil for drives, type 405 .................................................................. 14 0336 06086
2 micro-fuses 3.15 AT................................................................................................... 14 6000 04805
1 "Leica" brush, with magnet....................................................................................... 14 0183 40426
1 dust protective cover................................................................................................ 14 0212 30350
1 Instructions for Use printed (German/English,
with Language CD 14 0502 80200) .............................................................................. 14 0502 80001
The accessories are included at the top of the package (item 2, Fig. 9).
Check the delivery carefully against the packing list, delivery note and your order.
Should you find any discrepancies, please contact your Leica sales office without delay.
The country specific power cord needs to be ordered separately. Please find a list of all power
cords available for your device on our website www.LeicaBiosystems.com within the product
section.

16 Instructions for Use, V 2.2 RevI – 09/2016
4. Instrument Setup
4.3 Unpacking and installation
Fig. 9
When the instrument is delivered, check the tilt indicators on the
packaging.
If the arrowhead is blue, the shipment was transported laying flat, was
tilted at too great an angle or fell over during transport.
Note this on the shipping documents and check the shipment for pos-
sible damage.
Fig. 10
1
1
3
4
4
4
4
5
• Remove the packing strap and the ad-
hesive tape (1).
• Remove the carton lid (2).
• Take the accessory carton (optional ac-
cessories) (3) and the cartons (4) from
the standard scope of delivery.
The transport carton and in-
cluded retaining elements
should be kept in case a re-
turn shipment is necessary
later.
2

17
Leica RM2255
4.3 Unpacking and installation (cont.)
Fig. 10a
10
• Place the instrument on a stable laboratory
table.
The two sliding elements (10) located on
the rear of the base plate make it easier to
move the instrument on the table.
• To move the instrument, hold it by the front
of the base plate, lift it up gently and slide
it on its slides.
4. Instrument Setup
Never hold the instrument for
transport by the handwheel or the
rotary knob for section thickness
adjustment.
Observe the correct resting angle
to the table to avoid pinching your
fingers.
6
5
8
9
7
• Take out the fixing module (5). To do so, hold
it by the top edge of the module and in the
recessed grip (6) and pull it out by pulling
upwards.
• Remove the outer carton wall (7).
• Lift the instrument* (8) by holding it by the
base plate at the front and under the instru-
ment on the back and lift it out of the formed
cushion (9).
• (*= the instrument illustration is provided as
an example only.)

18 Instructions for Use, V 2.2 RevI – 09/2016
4. Instrument Setup
The feather key (4) is loosely placed in the hand-
wheel shaft (1) and fixed in place with a cable tie
during transport.
• Remove the cable tie (3). Caution!
Make sure not to lose the feather key!
• Place the handwheel (2) on the handwheel
shaft (1) as shown.
• Tighten the screw (2a) located in the center
hole of the handwheel with an Allen key size 4.
• Remove the cover foil from the self-adhesive
cover disk and fix the cover disk on the hand-
wheel.
4.4 Assembling the handwheel
4.5 Electrical connections
Checking the voltage
The Leica RM2255 can be connected to various
electrical power grids (depending on voltage and
frequency) and, for this reason, is always deliv-
ered with a set of various power cables.
New instruments are factory-set to 230 volts.
This is documented by a yellow label (230 VOLT)
on the rear of the instrument, which covers the
power switch and power socket.
2
2a
3
4
1
The handwheel has to be assembled before attempting to use the instrument.
The necessary parts and tools can be found in the toolkit.
Fig. 11
The instrument MUST be connected to a grounded power socket. Use only the power
cable provided that matches the power supply (outlets) of the country of use. Do not use
an extension cable without a ground wire!
Before connecting the instrument to the
power supply, be absolutely certain to
check that the voltage selector is set to
the voltage in use in your area!
Severe damage can be caused to the in-
strument if the voltage selector is set to
an incorrect voltage!
Never change the setting of the voltage
selector while the instrument is con-
nected to the power supply.