Leica DM100 User manual

Leica DM100
User Manual
Ryf AG
Bettlachstrasse 2
2540 Grenchen
tel [phone]
fax [phone]
www.ryfag.ch

Leica DM100 User Manual 2
Congratulations!
Congratulations on purchasing the Leica DM100
Compound Microscope. This model’s exclu-
sive design features and full range of acces-
sories make it a truly versatile, high quality-
instrument.
Though the reliability and robustness of
Leica microscopes is already practically prover-
bial, they still require a certain degree of care
and attention. Therefore, we recommend that
you read this User Manual. It contains all the
information you need regarding operation,
safety and maintenance. Simply observing a
few guidelines will ensure that even after years
of intensive use, your microscope will continue
to work as smoothly and reliably as on the very
first day.
We wish you the best of success in your work!

Leica DM100 User Manual 3
Important Notes
Symbols Used in this User Manual 5
Important Safety Notes 6
Instructions for Use 8
Health risks and dangers of use 10
Information for the Person Responsible for the Instrument 11
Accessories, maintenance and repair 12
Electrical data and ambient conditions 13
Assembling the Leica DM100
Unpacking 15
Identifying your Tube 16
Fitting the tube 17
Removing and Inserting Objectives 18
Installing the Mirror (optional) 20
Installing the Polarizer Kit (optional) 21
Using the Leica DM100
Switching on the Microscope 23
Selecting an Objective 24
Preparing for Viewing 25
Focusing 26
Adjusting the Binocular Tubes 27
Adjusting the Eyecups 29
Oil Immersion Technique (only 100× objective) 30
Care of the Microscope
Maintenance instructions 33
General maintenance 34
Dimensions
Dimensions 37
Table of Contents

Leica DM100 User Manual Important Notes 4
Important Notes

Leica DM100 User Manual Important Notes 5
Symbols Used in this User Manual
Warning! Safety hazard!
This symbol indicates especially impor-
tant information that must be read and
complied with.
Failure to comply can cause the following:
OHazards to personnel.
OInstrument malfunctions and damage.
Warning of hazardous electrical Voltage
This symbol indicates information that
must be read and observed.
Failure to comply can cause the following:
OHazards to personnel.
OInstrument malfunctions and damage.
Important Information
This symbol indicates additional infor-
mation or explanations that intend to
provide clarity.

Leica DM100 User Manual Important Notes 6
Important Safety Notes
Before installing, operating or using the
instrument, it is mandatory to read this
User Manual. In particular, please observe all
safety instructions.
The instruments and accessories
described in this User Manual have been
tested for safety and potential hazards.
Original condition
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in this User Manual.
Non-intended use
Using the instrument in any way other
than that described in this User Manual
may result in personal injury or damage to
property. Doing so can impair the functions of
the protective measures provided.
The responsible Leica affiliate must be
consulted whenever the instrument is
altered, modified or used in conjunction with
non-Leica components that are outside of the
scope of this User Manual! Unauthorized altera-
tions to the instrument or noncompliant use
shall void all rights to any warranty claims.
"Safety Concept" Brochure
The "Safety Concept" brochure contains addi-
tional safety information regarding service
work, requirements and the handling of the
microscope, the electrical and other accessories
as well as general safety instructions.

Leica DM100 User Manual Important Notes 7
Disconnecting from the Power Supply
Always position the Leica DM100 so
that you can disconnect it from the
power supply at any time. The power cable
must remain accessible at all times, because the
power cable is intended as a power disconnect
device.
Plug
Never install any other plug
(NEMA 5-15P) or unscrew any mechani-
cal components unless expressly instructed to
do so in the instructions.
Legal Regulations
Adhere to general and local regulations rela-
ting to accident prevention and environmental
protection.
EC Declaration of Conformity
Electrically operated accessories are construc-
ted based on the state of the art of technology
and are provided with an EC Declaration of
Conformity.
Important Safety Notes (continued)

Leica DM100 User Manual Important Notes 8
Instructions for Use
This User Manual describes the Leica DM100
and includes important instructions related to
operating safety, maintenance and accessories.
Your Leica DM100 includes an interactive
CD-ROM with all relevant User Manuals in
additional languages. Keep it in a safe place,
and readily accessible to the user. You can also
download User Manuals and updates from our
website at www.leica-microsystems.com.
Intended Use
The Leica DM100 is an optical instrument for
improving the visibility of objects through
magnification and illumination. It is used for
observing.
Place of use
The Leica DM100 microscope may be used only
in closed rooms and must be placed on a solid
surface.
The Leica DM100 can be used at +10°C to +40°C.
Protect the microscope from oil, chemicals and
extreme humidity. If it is used outdoors, the
microscope must be protected from dust and
moisture. Never use electrical instruments
outdoors and place them at least 10 cm away
from the wall and from flammable substances.
Avoid large temperature fluctuations, direct
sunlight and vibrations. These conditions
can distort measurements and micrographic
images.
In warm and warm-damp climatic zones,
the individual components require
special care in order to prevent the build-up of
fungus.

Leica DM100 User Manual Important Notes 9
Instructions on Use (continued)
Accessories from third-party suppliers
You can combine individual system articles with
articles from external suppliers. Please read the
User Manual and the safety requirements of the
supplier.
Integration in third-party products
When installing Leica products into third-party
products, the manufacturer of the complete
system or its dealer is responsible for following
all applicable safety instructions, laws and
guidelines.
Transport
If at all possible, use the original packaging for
shipping or transporting individual modules.
In order to prevent damage from vibrations,
all moving parts that (according to the User
Manual) can be assembled and disassembled
by the customer should be packed separately.
Disposal
Disposal must comply with locally applicable
laws and regulations.

Leica DM100 User Manual Important Notes 10
Health Risks and Dangers of Use
Health Risks
Workplaces with microscopes facili-
tate and improve the viewing task, but
they also impose high demands on the eyes
and hand muscles of the user. Depending on
the duration of uninterrupted work at such a
workplace, impaired vision and problems with
the musculoskeletal system may occur. For this
reason, appropriate measures for reduction of
the workload must be taken:
OOptimum workplace layout
OFrequent changes of activity
OThorough training of the personnel, giving
consideration to ergonomic and organiza-
tional aspects
The ergonomic optical design and construction
of the Leica microscopes are intended to reduce
the exertion of the user to a minimum.
Danger of Infection
Direct contact with eyepieces can be a
potential transmission path for bacterial
and viral infections of the eye.
The risk can be minimized by the use of
personal eyecups.
Dangers during Use
OThe Leica DM100 may be connected to a
grounded socket only.
OThe Leica DM100 may be used when it is in
proper working condition only.
LED Class 1 /LED 1 Laser Product
The illumination meets laser class 1 require-
ments in accordance with IEC 60825-1 "Safety
of Laser devices".
Caution: Hazardous radiation may occur if
controls, adjustment jigs, or procedures other
than those outlined in this User Manual are
used!
LED Class 1
Class 1
LED Product

Leica DM100 User Manual Important Notes 11
Information for the Person responsible for the Instrument
Information for the Person responsible for
the Instrument
OEnsure that the Leica DM100 is used only by
persons qualified to do so.
OEnsure that this User Manual is always avail-
able at the place where the microscope is
in use.
OCarry out regular inspections to make
certain that the authorized users are adher-
ing to safety requirements.
OWhen instructing new users, do so thor-
oughly and explain the meanings of the
warning signs and messages.
OAllocate responsibilities for commission-
ing, operation and maintenance. Monitor
compliance with this.
ODo not use the Leica DM100 unless it is in
perfect condition.
OInform your Leica representative or Leica
Microsystems (Schweiz) AG, Industry
Division, 9435 Heerbrugg, Switzerland,
immediately of any product defect that
could potentially cause injury or harm.
OIf you use accessories from other manu-
facturers with the microscope, make sure
that these manufacturers confirm that
the combination is safe to use. Follow the
instructions in the User Manual for those
accessories.
OModifications to or service on the micro-
scope may be carried out only by techni-
cians who are explicitly authorized by Leica
to do so.
OOnly original Leica replacement parts may
be used in servicing the product.
OAfter service work or technical modifica-
tions, the unit must be reconfigured with
observance to our technical requirements.
OIf the unit is modified or serviced by unau-
thorized persons, isimproperly maintained
(as long as maintenance was not carried
out by Leica Microsystems), or is handled
improperly, Leica Microsystems will not
accept any liability.
OThe building’s electrical wiring must
comply with the relevant national stand-
ard; in other words, we recommend using a
current-operated ELCB system (RCB).

Leica DM100 User Manual Important Notes 12
Accessories, Maintenance and Repair
Accessories
Only the following accessories may be used
with the Leica DM100:
OThe Leica Microsystems accessories
described in this User Manual.
OOther accessories, provided that these have
been expressly approved for use by Leica
Microsystems with regard to safety tech-
nology.
Maintenance
OThe Leica DM100 microscope is basically
maintenance-free. To ensure that it always
operates safely and reliably, we recommend
that you take the precaution of contacting
the responsible service organization.
OWe recommend concluding a service
contract with Leica Microsystems.
Repairs and Service Work
OOnly original Leica Microsystems spare
parts may be used.
OBefore opening the instruments, switch off
the power and unplug the power cable.
OAvoid contact with powered electrical
circuits, which can lead to injury.
Service Address
In case of problems, please contact us as
follows:
Contact address
Leica Microsystems (Schweiz) AG
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)

Leica DM100 User Manual Important Notes 13
Electrical Data and ambient Conditions
In order to maintain this condition and to ensure safe operation,
the user must follow the instructions and warnings contained in
this instruction manual.
Environment
Temperature for use +10 °C … +40 °C
Storage temperature -20 °C … +52 °C
+50 °F to +104 °F
Manipulation shock 25 mm on 50 mm hard wood
Transport shock (unpacked) 100 g / 6 ms
Transport shock (packed) 800 mm free fall
Transport vibrations (unpacked) 5–200 Hz / 1.5 g
Air pressure during use and storage 500–1 060 mBar
Humidity during use and storage 20–90 %
Installation Category II (Overvoltage Category)
Pollution degree 2
Fuse Replacement
Unplug the instrument before changing any fuses. The
Leica DM100 has two fuses, which are located behind the power
cord receptacle.
Only use fuses of the following type: 5×20 mm, 1.0 A/250 V, high
breaking capacity (# 13RFAG30003)
Electrical data
Input: 100–240 V, 50/60 Hz, 5 W (3 W LED)
General safety notes
This instrument of safety class 1 has been built and tested in accordance
with the following safety requirements for electrical equipment for measu-
rement, control, and laboratory use:
EN 61010-1: 2002-08
EN 61010-2: 2008-06
IEC 61010-1: 2010-06
EN 61326-1: 2006-10
EN 61326-2-6: 2006-10
EN 55011: 2007+A2: 2010-05
EN 60825-1: 2008-05
IEC 60825-1: 2007-03
LED Class 1

Leica DM100 User Manual Assembling the Leica DM100 14
Assembling the Leica DM100

Leica DM100 User Manual Assembling the Leica DM100 15
Unpacking
OCarefully remove the microscope and any
components from the packaging.
OCheck the components against the planned
configuration.
OOptional items such as contrast accessories,
camera adapters, cameras, and carrying
cases are not shipped as part of the stan-
dard equipment. These items are delivered
in separate packages.
OPlease do not discard any of the packing
materials. They should be used for safely
storing and transporting the instrument
should the need arise.

Leica DM100 User Manual Assembling the Leica DM100 16
Identifying your Tube
Identifying your ube
There are three types of viewing tubes.
Identify which viewing tube you have:
Assembly
Installation of the tube is described
on page 17 using the monocular
"EZTube". Other tubes, however, are installed
exactly the same way.
Orientation
All tubes are connected via a dovetail
and can be installed in any orientation.
Type 1: Monocular "EZTube"
Type 3: Open binocular tube
Type 2: Binocular "EZTube"

Leica DM100 User Manual Assembling the Leica DM100 17
Fitting the tube
Tools used
OAllen key
The monocular "EZTube" is included in
the standard delivery package of the
Leica DM100. Other tubes are optionally availa-
ble (refer also to page 16). Your Leica repre-
sentative will gladly provide you with more
help.
The eyepieces are already integrated in
the Leica "EZTube" and preset; for this
reason, the eyepieces do not require adjust-
ment or assembly.
Assembly
1. Loosen the setscrews (on top of the stand)
using the Allen key provided.
2. Insert the dovetail of the monocular tube
into the mount.
3. Turn the tube in the direction you desire.
4. Tighten the set screws on the left and right,
without applying too much force.

Leica DM100 User Manual Assembling the Leica DM100 18
The objectives are already installed
on the Leica DM100 at delivery. The
following description shows how to (un)install
the objectives if they have to be removed for
cleaning or replaced by other objectives.
Your Leica DM100 is normally supplied
with objectives of magnification levels
4×, 10×, and 40×. The 100× objective, which is
also used for oil immersion, can be retrofitted
at any time.
Always turn the objective nosepiece by
its knurled ring. Never turn the objective
nosepiece by using an objective as a handle.
Removing Objectives
1. Unscrew the objectives to remove them.
The Proper Sequence
When inserting the objectives, you can
begin with any available space on the
microscope. Continuation of the sequence,
however, has to be selected such that the
magnification always increases when turning
clockwise:
Continued on next page.
Removing and Inserting Objectives
4× 40×
100×
10×

Leica DM100 User Manual Assembling the Leica DM100 19
Inserting Objectives
1. Screw the 4× objective into any available
space on the objective nosepiece.
2. Turn the objective nosepiece clockwise one
unit.
3. Screw in the next-largest objective.
4. Repeat steps 2 and 3 until all objectives
have been installed.
Removing and Inserting Objectives (continued)

Leica DM100 User Manual Assembling the Leica DM100 20
Installing the Mirror (optional)
The mirror is available as an optional
accessory. By means of this, the ambient
light can be used for illuminating the sample if
no power source is available for the built-in LED
illuminator.
The mirror is equipped with a flat and
a concave surface. Try them out to see
which side works better.
Installing the Mirror
1. Position the microscope at a well-lit loca-
tion, for example before a window.
2. Press the mirror onto the mount of the illu-
minator.
3. Look through the eyepieces and align the
mirror so that it works best.
Removing the Mirror
1. Simply pull the mirror out of the holder.