Leica M-Series User manual

Leica M-Series
Stereo-
microscopes
User Manual

2
Contents Page
Overview
Safety concept . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
Controls and functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Short instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Use
Ergonomics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Viewing height and interpupillary distance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Eyepoint, eye contact . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Changing the magnification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Focusing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Stereoscopic and axial observation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Dioptric settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Working with the stereomicroscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Assembly
Basic outfit
Overview: Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Spacer rings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Fitting the column to the incident- and transmitted-light bases . . . . . . . . . . . . . . . 18
Microscope carrier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Binocular tubes, optics carrier, optical accessories . . . . . . . . . . . . . . . . . . . . . . . . 19
Graticules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20
Stands
Transmitted-light stand, bright field, 20W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Transmitted-light stands, bright and dark field . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Swinging-arm stand ESD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Large swinging-arm and table-clamp stands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Illuminators
Transformers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Leica L2 cold-light source . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
Fibre-optic light guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Cold-light source Leica CLS Series . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
Mains (line) lamp 25W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Incident lamp 6V/10W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Incident lamp 6V/20W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Coaxial illuminator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Near-vertical illuminator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

3
Page
Accessories
Fitting accessory tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Double-iris diaphragm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Drawing tube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Video-phototubes: Photography, video, TV, filming, digital imaging . . . . . . . . . . . . 34
Attachment for vertical and oblique observation . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Stages, polarization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Special notes
Tips and hints: What to do if … . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Care and maintenance of the stereomicroscope, maintenance . . . . . . . . . . . . . . 39
Calculating total magnification and field diameter . . . . . . . . . . . . . . . . . . . . . . . . . 40
Optical data, MS5, MZ6 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Optical data, MZ75, MZ95. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Optical data, MZ125, MZAPO . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

4
Prohibited uses
• The use of the instrument in a different manner from that
described in this user manual can lead to injury, malfunction
and damage.
• Do not fit different plugs. Do not dismantle optical systems or
mechanical parts unless instructions for doing so are given
in the user manual.
Place of use
• The Leica MS5, MZ6, MZ75, MZ95, MZ125and MZAPO
stereomicroscopes are intended mainly for indoor use.
• If the stereomicroscope is used outdoors, protect it from dirt
and damp. Lamps and electrically-operated stands from
Leica may not be used outdoors.
Use in ESD protected rooms
The optics carrier MS5, MZ6, MZ75, MZ95, MZ125, the binocular
tube, ErgoTubus™, ErgoKeil™ 5°–25°, the swinging-arm stand
ESD and the cold light sources Leica L2 and CLS consist of ESD-
discharging material (surface resistance (<1011 ohms/squared,
discharge time <2 seconds, 1,000V to 100V).
• A connection socket for a 4mm dia. earthing cable is
provided on the microscope carrier.
• There are two connection sockets for 4mm dia. earthing
cables in the base of the swinging-arm stand.
Servicing
Repairs may only be carried out by Leica-trained service
technicians. Only original Leica spare parts may be used.
Responsibilities of person in charge of instrument
• Ensure that personnel who use the instrument have read
and understood this user manual and in particular the safety
instructions.
• Ensure that the Leica MS5, MZ6, MZ75, MZ95, MZ125and
MZAPO stereomicroscopes are operated, maintained
and repaired only by authorized and trained personnel.
To you, the user
Thank you for your trust. We wish you success and satisfaction
in your work with your new stereomicroscope from Leica.
The innovative technology of the new stereomicroscope line
from Leica is expressed externally in the avant-garde form of the
instrument. The rounded shapes, with their elliptical tendencies,
demonstrate that beauty can be practical.
In developing this new stereomicroscope line we have placed
great weight on simple, self-explanatory directions. We have
however put a lot of effort into explaining all functions to you by
means of pictures and text. You will only get the best out of
your stereomicroscope if you take the trouble to study this user
manual in detail.
If you still have a question sometime, just contact your local
Leica agency or get in touch directly with Leica Microscopy
Systems Ltd, Heerbrugg, Switzerland. We are gladly at your
service. Customer service is a big thing with us. Not only before
the sale, but afterwards as well.*
* The business units of Leica Microsystems hold
the management system certificates for the international
standards ISO 9001 and ISO 14001 relating to quality
management, quality assurance and environmental
management.
Safety concept
General directions
Before you try to use the instrument, please read the user
manual and the safety notes.
Permitted uses
The Leica MS5, MZ6, MZ75, MZ95, MZ125and MZAPO stereo-
microscopes are optical instruments which use magnification
to see the finer details of objects close to the eye.
The objects may be either natural, or created by human agency.
The basic outfit, which includes a stand and a light source,
can be supplemented with various modules for photography,
TV, dual station viewing and other applications.

5
Safety regulations
Electrically-operated items
The safety regulations listed below apply to the following items:
– Transmitted-light stand, bright field, 20W (page 20)
– Mains lamp 25W (page 28)
– Regulating transformer 0–7V/40W (page 26)
– Step transformer 4/5/6V (page 26)
– Motor focus (separate instructions)
Liquids
Be careful when handling liquids. If spilt on the equipment,
they can:
– cause the stereomicroscopes and accessories to become
electrically live, endangering personnel
– cause damage to the equipment.
Power cables
Inspect cables regularly for damage. Defective cables can:
– directly endanger personnel
– cause the stereomicroscopes and accessories to become
electrically live, endangering personnel.
Positioning cables
Position cables with care. Make sure that personnel do not get
entangled with them. The instrument could fall over, fall down,
become damaged, damage other equipment, or cause personal
injury.
Opening the instrument
Only authorized Leica personnel are permitted to repair
electrical equipment.
Before opening up the equipment, pull out the power cable.
If you touch the live interior of the equipment, you may receive
an electric shock.
Grid voltage
Ensure that the voltage setting on the equipment is correct.
A wrong setting can cause damage to the equipment.
Connections
When connecting power-consuming devices to the trans-
formers, do not exceed the maximum permitted loading, as this
can cause damage to the equipment.
Changing bulbs
• Before changing a bulb, disconnect the power cable.
• Never attempt to touch the inside of a lamp housing or
transmitted-light stand while the instrument is connected
to the power supply.
• Before changing a bulb, wait until it is cool, otherwise you
may burn your fingers.
Legal requirements
Adhere to general and local regulations relating to accident
prevention and environmental protection.
Conformity with European Community directive
The electrically-operated accessories for the Leica MS5, MZ6,
MZ75, MZ95, MZ125and MZAPO stereomicroscopes are
constructed in accordance with the latest technologies and are
provided with a statement of conformity with EC requirements.
You will find these symbols in the user manual:
Safety direction
This symbol denotes information which must be read
and obeyed.
Failure to respect it can cause injury
Failure to respect it can cause malfunctioning,
or damage to the equipment
Warning about accessible hot places, e.g. bulbs
Failure to respect it can cause burns
Important information
This symbol denotes additional information
or explanations which promote understanding.
Action
왘This symbol within the text indicates that certain operations
must be carried out.
Explanatory notes
• This symbol within the text stands for additional information
and explanations.

6
Overview
Standard outfit: Controls and functions
In this overview
we explain the controls and functions relating to the standard
outfits.
Does your stereomicroscope look different from the one
in the picture?
The modular design enables you to assemble an outfit matched
to the application. Controls and functions which differ from
those in the standard outfit are described in the appropriate
section (see contents list on page 2).
Leica MZAPO see page 8.
Stereomicroscope
1 Magnification changer
Drive knobs with magnification scale.
MS5: 5 steps
MZ6: 6:1 zoom
MZ75: 7.9:1 zoom
MZ95: 9.5:1 zoom
MZ125: 12.5:1 zoom
MZAPO: 10:1 zoom.
2 Engaging ratchet positions
MZ6: Switch engages 7 magnification steps
MZ75: Milled ring engages 8 magnification steps
MZ95: Switch engages 9 magnification steps
MZ125: Switch engages 10 magnification steps
MZAPO: Switch engages 9 magnification steps.
3a Focusing drive
3b Coarse and fine focusing
Larger knob: Coarse focusing
Smaller knob: Fine focusing.
3c Adjustable ring
Changes the ease of movement of the coarse/fine drive.
4 Clamping screw
Holds the optics carrier in the microscope carrier.
5 Clamping screw
Holds the binocular tube (or accessory) to the optics carrier.
6 Interchangeable objective
Various objectives (achromatic, planachromatic,
planapochromatic), depending on the outfit.
Binocular tube
7 Adjustable eyepiece tubes:
Interpupillary distance adjustable within the range
52mm – 76mm.
8 Wide-field eyepieces for spectacle wearers
Dioptric setting adjustable within the range from +5 to –5,
and eyecups adjustable.
9 Clamping screws
These hold the eyepieces in the binocular tube.
10 ErgoTube™
The viewing angle is adjustable within the range 10° – 50°.
MS5
9
7
5
4
6
1
3a
8
Leica Design
by Ernest Igl/Christophe Apothéloz

7
MZ6
MZ95MZ125
MZ75
9
7
5
4
6
1
2
3a
8
7
5
4
6
2
1
3c
3b
8
7
5
4
6
2
1
3c
3b
8
7
5
10
4
6
2
1
3c
3b
8

8
Short instructions
Checklist
In this overview
we have summarized the working sequence. You will find
detailed user instructions on the pages indicated.
Inspect the cleanliness
왘Remove dust and dirt from the instrument (page 39).
왘Clean: Optical accessories, glass stage plates, and any
graticules used (page 39).
Only for variable ErgoModules™
왘Adjust viewing height (page 9)
Only for photomicrographic outfits
왘Lever at position “VIS”.
왘For trinocular tube: Push diaphragm lever to the right.
왘Open double-iris diaphragm (position “OPEN”).
Adjust the interpupillary distance
왘Using both hands, displace the eyepiece tubes (page 9).
Select the degree of eye contact
왘Secure the eyecup in the position required (page 9).
Adjust the dioptric setting
왘Adjust the dioptric setting precisely for each eyepiece
(page 12).
Set the magnification required
왘Turn the rotary knob to the desired magnification (page 10).
Focusing
왘Look into the eyepieces and focus on the object (page 11).
• Only for coarse/fine drive:
Focus approximately with the large knob, and then
accurately with the small knob.
왘If the focusing movement is too loose or too tight, adjust the
ease of movement (page 11).
Adjust the depth of field
왘Adjust the depth of field with the double-iris diaphragm
(page 33).
Microscope carrier AX
왘For measuring, photography and polarization, engage the
vertical beam path (page 12).
MZAPO
7
5
10
4
6
9
1
2
3b
3c
8

9
Use
Ergonomics, viewing height, interpupillary distance,
eye contact
Ergonomics
The reasons why users often cannot work comfortably with the
45° tube are the differing heights of the outfits, the various
accessories and working distances, and the fact that the same
instrument is often used by several people of differing builds.
This problem can be effectively solved by using the Leica
ErgoModules™.
If you have problems with the viewing configuration on your
stereomicroscope, ask your Leica consultant for the most
ergonomic Leica solutions.
Viewing height, viewing angle
• The viewing height is correct when your head and back are
comfortably positioned.
• On the ErgoTube™, the viewing
angle is adjustable from 10° –
50°.
왘Holding the eyepiece tubes at
their bases, swing them up or
down (see arrow).
ErgoModule™
• ErgoWedge™ 5° – 25°
• ErgoWedge™ ±15°
• ErgoModule™ 50mm
• The ErgoTube™ 45° has eyepiece tubes which are 65mm
longer than the standard ones.
• ErgoModule™ 30mm – 120mm
• After several years, regreasing
may be necessary. Send the
ErgoModule™ to the Leica
service workshop.
Interpupillary distance
• The interpupillary distance is correct when you see a single
circular field when viewing with both eyes.
왘Look into the eyepieces and use
both hands to move the eye-
piece tubes closer together or
further apart.
Locating the eyepoint
• For 10×/21B wide-field eyepieces for spectacle wearers,
the distance from eye to eyepiece is about 22mm.
• When you can see the entire field of view without restriction,
you have found the correct position (eyepoint).
왘To find the eyepoint, slowly bring
your eyes closer towards the
eyepieces.
Selecting the degree of eye contact
If you do not wear spectacles, and want closer contact to the
eyepieces:
왘Holding the diopter ring, turn the
eyecup anticlockwise until it is
released.
왘Pull the eyecup upwards.
왘Holding the diopter ring, turn the
eyecup clockwise.
Spectacle wearers need the whole
of the available distance. Move
the eyecups to the lowest position.

10
Use
Magnification changer
MS5: 5 steps
MZ6: 6:1 zoom
MZ75: 7.9:1 zoom
MZ95: 9.5:1 zoom
MZ125: 12.5:1 zoom
MZAPO: 10:1 zoom
•Zoom means continuous change of magnification. Ratchets
can be engaged for certain magnifications (see below).
Changing the magnification
• The magnification changer can be operated from either the
right or the left.
왘Look into the eyepieces.
왘Focus on the object (page 11).
왘Turn the magnification changer
to the position required.
Ratchet steps
MZ6: 0.8, 1.0, 1.25, 1.6, 2, 2.5, 3.2
MZ75: 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4
MZ95: 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5
MZ125: 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5, 6.3, 8
MZAPO: 10, 12.5, 16, 20, 25, 32, 40, 50, 63
• Engage the ratchets whenever magnifications need to be
exactly reproducible.
왘For MZ6, MZ95, MZ125and
MZAPO: Press the switch.
왘For MZ75: Turn the knurled ring
anticlockwise.
Scale of magnifications
MS5, MZ6
Display of magnification factors from 0.63 – 4.
MZ75
Display of magnification factors from 0.63 – 5. For other objective-
eyepiece combinations, appropriate adhesive scales can be
applied to the rotary knobs (page 19).
MZ95
Display of magnification factors from 0.63 – 6.
MZ125
Display of magnification factors from 0.8 – 10.
MZAPO
Display of magnifications with various optical combinations,
e.g. with 1.0×objective and 10×eyepieces: 8×– 80×.
MZAPO: Setting the scale
The magnification scale can be set in accordance with the
optical combination.
Example:
1.0×objective / 25×eyepiece
왘Turn the magnification changer
to the stop at its lowest magnifi-
cation position.
왘Read the magnification range
from the table on page 43 (in the
example given, 20×to 200×).
왘Place a finger in the rear
window of the magnification
changer and displace the
scale so that the position 20
is lined up with the index.
Magnifications and fields of view
The tables between page 41 and page 43 provide information
about the magnifications and field diameters as a function
of the magnification changer position of the eyepiece-objective
combination used.

11
Magnification with MZ125/MZAPO Pan and Planapo objectives
When the MZ125/MZAPO planachromatic and planapochromatic
objectives are used on the Leica MS5, MZ6, MZ75and MZ95,
the magnification is increased by the factor 1.25×. This factor
is taken into account in the tables.
Setting the working distance, focusing = obtaining a sharp picture
• To focus, use the focusing drive to raise or lower the
stereomicroscope until the feature of interest is in focus.
The working distances of the various objectives are listed in
the tables on pages 41–43.
• The focusing drive can be operated from either the right or
the left.
왘Place the object beneath the
objective.
왘Engage the lowest magnification
(page 10).
왘Look into the eyepieces.
왘Using the rotary knob, bring the
object into focus.
Only for coarse / fine drives
왘Focus approximately with the
larger knob.
왘Focus accurately with the
smaller knob.
Adjusting the free running of the focusing drive
Does the focusing drive run too loosely or too tightly, or does
the equipment drift downwards?
You can match the ease of movement to the weight of the outfit
and to your personal requirements.
Focusing drive
왘Grip the drive knobs firmly with
both hands and turn them in
opposite directions until you
obtain the required degree of
resistance during focusing
Focusing drive, coarse / fine
왘Turn the black ring on the
right-hand drive knob until you
obtain the required degree of
resistance during focusing.
Magnification on Magnification on
MZ125/MZAPO MS5/MZ6/MZ75/MZ95
1.0× Plan/Planapo 1.25×
1.6× Planapo 2.0×
0.63×Planapo 0.8×
0.5×Plan 0.63
0.8×Plan 1.0×

12
Use
Microscope carrier: Turn optics carrier sideways,
stereoscopic / axial observation
Microscope carrier for stereoscopic observation
This microscope carrier permits only stereoscopic observation,
using both beam paths.
• The optics carrier can be turned sideways in the microscope
carrier if the user needs to work from the side:
왘Release the clamping screw.
왘Turn the optics carrier sideways
as required.
왘Retighten the clamping screw.
The microscope carrier can be attached to the focusing drive in
either of two positions:
• For short working distances and
for flat objects, use Position 1.
Use this position also in con-
junction with the attachment for
vertical and oblique observation
(see detailed instructions).
• Use Position 2 for long working
distances and for bulky objects.
For assembly, refer to page 18.
Microscope carrier AX
The stereomicroscopes can be equipped with the microscope
carrier AX for stereoscopic and axial observation instead of the
standard microscope carrier.
• The microscope carrier cannot be turned sideways when in
the microscope carrier AX (see page 24).
• The microscope carrier AX can only be fitted in one position
to the focusing drive (see page 18).
For the Leica MZ125and Leica MZAPO, use the spacer ring
(page 16).
Stereoscopic observation
A three-dimensional image is essential for studying and working
on spatial objects.
왘Shift the stereomicroscope to the central position.
Axial observation
The parallax-free imaging produces more precise results in
photography, measuring and polarization.
왘Shift the stereomicroscope to
the left or the right position. The
object is now located directly
beneath either the left or the
right beam path.
• When photographing or measur-
ing, select the beam path above
which the phototube or measur-
ing graticule is located.
Adjusting the dioptric settings and the parfocality
You must adjust the dioptric settings for both eyes
exactly as described on page 13. Only then will the
image be parfocal, i.e. it will remain sharp whenever
the magnification is changed and there will be no need
to refocus. Make the most of this opportunity, which is
not offered by every stereomicroscope.
• The dioptric range is from
+5 to –5.
1
2

13
The following adjustments must be carried out only once
by each user.
The procedures for adjusting the dioptric setting and the
parfocality are somewhat different if an eyepiece contains a
graticule. Refer to the user manuals for the MPS modular
photomicrographic systems or for measuring.
A Preparations
왘Set up the illumination (pages 20–21, 26–32).
왘Set the interpupillary distance and, if necessary, the viewing
height (page 9).
왘On the video-/phototube: Move the lever to position “VIS”
and open the diaphragm.
왘Set the double-iris diaphragm to
“OPEN” (page 33).
왘Adjust the eyecups (page 9).
왘Set both eyepieces to zero.
왘Set the microscope carrier AX
for stereoscopic observation.
B Focus on the test object
1. Flat test object
왘Place such an object beneath
the objective.
2. Lowest magnification
왘Engage the lowest magnification
position (page 10).
왘Use the coarse focusing drive
to set the working distance
(page 11).
3. Look into the eyepieces
왘Using the focusing drive, bring
the test object into focus.
4. Highest magnification
왘Engage the highest magnifica-
tion position.
5. Image sharpness
왘Optimize with focusing drive.
C Adjust the dioptric settings
6. Lowest magnification
왘Engage the lowest magnification
position.
7. Do not look into the eyepieces
왘Rotate the eyepieces anticlock-
wise in the “+” direction as far
as the stop (+5 diopters).
8. Look into the eyepieces
왘Slowly rotate each eyepiece
individually in the “–” direction
until each eye sees the object
sharply imaged.
D Inspect the parfocality
9. Highest magnification
왘Engage the highest magnifica-
tion position.
10. Refocus
왘Refocus if necessary.
11. Magnification changer
왘Slowly move the magnification
changer from the lowest
magnification to the highest.
•The image sharpness must
remain constant (parfocal).
Otherwise, repeat the
procedure.

14
Use
Working with the stereomicroscope
In this section
we will show you how to work smoothly with your stereomicro-
scope and how to study objects routinely.
Preconditions for working comfortably
• Pay great attention when setting up your stereomicroscope.
You will only benefit fully from the outstanding optical and
ergonomic advantages of the instrument if you set it up
precisely in accordance with the directions already given.
• For fatigue-free work, use the ErgoModules™ (page 9).
• Arrange your workplace as well as possible, and pay
attention to the heights of chair and table.
• When sitting, use the entire seat area and also the back
support of the chair.
• Support your forearms.
• During work breaks, stand up and move around for exercise.
Tips for your work
The stereomicroscopes are parfocally matched. This
means that, once you have focused on a feature in the
manner described, taking account of dioptric settings
(page 12), you can zoom the magnification up and down
without refocusing. You only need to refocus if you
want to look at higher or lower planes in the object.
• When focusing on a fresh object, always start with the
lowest magnification, because the larger field of view makes
the object easier to find. Afterwards, select the magnification
you want.
왘Place the object beneath the
objective.
왘Illuminate the object.
왘Select the lowest magnification
position.
왘Using the focusing drive, set the
working distance approximately
(page 11).
왘Look into the eyepieces and use
the focusing drive to bring the
object precisely into focus.
왘Select the highest magnification
position.
왘Use the focusing drive to
refocus.
Now the object should remain in
focus at all zoom magnifications.
Otherwise, check that your dioptric
settings have been adjusted exactly
as described on page 12.

15
1a
2a
2b
1b
7
6
4
5
3
8
Assembly
Basic outfit
The components
1a Incident-light base with stage plate
1b Transmitted-light base with glass stage plate
2a Side-faced column with drive housing, coarse/fine
2b Side-faced column with drive housing
3 Microscope carrier
4 Optics carrier
5 Interchangeable objective
6 Binocular tube
7 Wide-field eyepiece for spectacle wearers, with eyecups
8 Various ErgoModules™ or accessories for video,
photography, drawing, fluorescence, coaxial incident
light etc.

16
Spacer rings
Spacer rings are required in order to attach the objectives
for certain instrument configurations.
• The Leica MZ95is supplied with a spacer ring for the
smaller-diameter achromatic objectives and 1.0×plan-
achromatic objective. When the spacer ring has been
removed by applying the tool provided, the larger-diameter
planachromatic and planapochromatic objectives for
the MZ125/MZAPO can be used.
• The Leica MS5, MZ6 and MZ75can also be equipped with
the larger-diameter planachromatic and planapochromatic
objectives for the MZ125/MZAPO if a spacer ring is fitted.
• When the MZ125/MZAPO planachromatic and planapo-
chromatic objectives are used on the Leica MS5, MZ6, MZ75
and MZ95stereomicroscopes, the magnification is increased
by a factor of 1.25×.
10 445 614 MZ6
10 411 589 1x
10 422 562 1.5x
10 422 561 2x
10 473 832 0.8x
10 445 201 0.63x
10 422 563 0.5x
10 422 564 0.32x
Achromat
10 446 371 MZ75
10 446 272 MZ95
10 446 370 MZ125
10 445 821 MZAPO
10 445 613 MS5
10 446 157 0.5x
10 446 230 1x
10 472 650 1.6x
10 446 236 0.63x
10 447 075 0.8×
10 446 172
10 411 589 1x
10 422 562 1.5x
10 422 561 2x
10 473 832 0.8x
10 445 201 0.63x
10 422 563 0.5x
10 422 564 0.32x
Achromat
10 446 275 1x
Plan
10 446 275 1x
Plan
10 445 819 1x Plan
10 446 157 0.5x
10 446 230 1x
10 472 650 1.6x
10 446 236 0.63x
Plan/Planapo
10 445 352
10 315 306
10 367 929
10 445 352
10 315 306
10 367 929

17
Article no. MS5 MZ6 MZ75MZ95MZ125MZAPO
Achromat
Achromat 1×10 411 589 C C C C
Achromat 1.5×10 422 562 C C C C
Achromat 2×10 422 561 C C C C
Achromat 0.8×10 473 832 C C C C
Achromat 0.63×10 445 201 C C C C
Achromat 0.5×10 422 563 C C C C
Achromat 0.32×10 422 564 C C C C
Plan
Plan 1×10 446 275 C C C C
Plan 1×MZ125/MZAPO 10 445 819 CA (10 446 172) M CA (10 446 172) M CA (10 446 172) M C*M C C
Plan 0.5×MZ125/MZAPO 10 446 157 CA (10 446 172) M CA (10 446 172) M CA (10 446 172) M C*M C C
Plan 0.8×10 447 075 CA (10 446 172) M CA (10 446 172) M CA (10 446 172) M C*M C C
Planapo
Planapo 1×MZ125/MZAPO 10 446 230 CA (10 446 172) M CA (10 446 172) M CA (10 446 172) M C*M C C
Planapo 1.6×MZ125/MZAPO 10 472 650 CA (10 446 172) M CA (10 446 172) M CA (10 446 172) M C*M C C
Planapo 0.63×MZ125/MZAPO 10 446 236 CA (10 446 172) M CA (10 446 172) M CA (10 446 172) M C*M C C
Accessories
Coaxial incident light 10 446 180 C C O (10 446 300) O (10 446 300) C C
Microscope carrier AX 10 445 618 C C C C CA (10 446 172) CA (10 446 172)
Near-vertical illuminator 10 445 198 C C CA 2×(10 446 300) CA (10 446 300) CA (10 446 300) CA (10 446 300)
+ (10 446 393) + (10 446 393)
Attachment for vertical 10 445 156 C C CA 2×(10 446 300) CA (10 446 300) CA (10 446 300) CA (10 446 300)
and oblique observation + (10 446 393) + (10 446 393)
C Compatible
CA Compatible if used with intermediate ring (order separately)
0 Intermediate ring recommended
M Magnification increased by factor 1.25×
* Remove intermediate ring (10 446 393); already included with MZ95.
Objective combinations

Microscope carrier, optics carrier, binocular tube,
optical accessories
In this section
we will explain how to fit the microscope carrier and the optics
carrier to the incident- and transmitted-light stands.
The combination of the optics carrier with the inclinable
drive housing is described on page 22.
The Leica motor focus system is described in a separate user
manual.
Side-faced column ➜drive housing
The microscope carrier can be fitted in either of two positions to
the drive housing (refer also to page 12):
왘Use an Allen screw to secure
the microscope carrier in the
position required.
A connection socket for a 4mm dia.
earthing cable is provided on the
microscope carrier.
Microscope carrier AX
• The microscope carrier AX can be fitted to the drive housing
only in position 1 (see above).
• The optics carrier cannot be turned sideways in the
microscope carrier AX.
• The Leica MZ125and Leica MZAPO require a spacer ring
(page 16) in order to secure the planachromatic and
planapochromatic objectives to the microscope carrier AX.
18
Assembly
In the following sections
we will explain how to assemble a complete stereomicroscope
outfit, including the incident- and transmitted-light stands.
Detailed directions about the transmitted-light stands, the
swinging-arm stand and the fitting of the remaining accessories
are all described in the appropriate sections (see contents list
on page 3).
Side-faced column ➜incident-light base
왘Remove the stage plate.
왘Introduce 3 Allen screws into
the underside of the baseplate
and secure them to the bottom
end of the side-faced column.
왘Insert the stage plate.
The side-faced column with focusing drive is available with
column lengths 300mm and 500mm.
To fit the microscope carrier and the stereomicroscope, see the
next column of text.
Side-faced column ➜transmitted-light base
Transmitted-light stand, bright field, 20W:
Pull the power cable out of the socket in the stand.
•Transmitted-light stand for bright and dark field: Pull the
fibre-optic light guide out of the connector in the stand.
왘Remove the glass stage plate.
왘Carefully invert the transmitted-
light base and remove 7 Allen
screws from the base.
왘Lift off the lower part of
the base.
왘Reverse the upper part of
the base.
왘Introduce 3 Allen screws into
the cover plate from beneath
and secure them to the bottom
end of the side-faced column.
왘Replace and secure the base.
왘Insert the glass stage plate.
왘Fit the microscope carrier and
stereomicroscope as follows:

19
Optics carrier ➜microscope carrier
왘Gently lower the optics carrier
into the microscope carrier.
왘Use the screw to secure the
optics carrier in the position
required.
Apply the adhesive scale of magnifications
Only for MZ75:
왘Remove the screws.
왘Remove the rotary knobs.
왘Apply the adhesive scales.
왘Replace the rotary knobs.
Objective
왘Screw on the selected
objective anticlockwise.
• Some instrument configurations require spacer rings in order
to secure the objectives (page 16). These rings are fitted
and removed using the tool provided.
• The Leica MZ95is supplied with a spacer ring for the
achromatic objectives and for the 1.0×planachromatic
objective. When the ring has been removed by means
of the tool provided, the larger-diameter planachromatic
and planapochromatic objectives for the MZ125/MZAPO
can be fitted.
Binocular tube
왘Release the clamping screw.
• If you have an accessory such
as an ErgoModule™ (page 9),
a coaxial illuminator (page 32),
a video-/phototube (page 35)
or a filter-slide holder (page 35),
fit it now to the optics carrier.
Tighten the clamping screw.
왘Tilt the binocular tube and insert
the edge of its dovetail ring
beneath the two lugs on the
optics carrier.
왘Move the binocular tube from
side to side until the locating
screw on the optics carrier
engages the guide.
왘Tighten the clamping screw.
Eyepieces
왘Insert a graticule if appropriate
(page 20).
왘Release the clamping screws on
the eyepieces.
왘Insert the eyepieces fully into
the eyepiece tubes. Check that
they have seated correctly.
왘Tighten the clamping screw.
• You can extend the overall magnification range by using 16×,
25×and 40×wide-eyepieces for spectacle wearers.

20
Graticules
• The graticules for measuring and counting are in mounts
which can be fitted directly into the eyepieces.
• A highly-precise stage micrometer with a 50mm scale and
0.1mm and 0.01mm divisions enables the graticule to be
calibrated in accordance with the magnification selected.
• It is best to measure with the microscope carrier AX in
vertical position. The measurements are parallax-free and
therefore more precise.
• Focusing and framing graticules indicate the format limits
for all films.
Fitting the graticule
왘Unscrew the sleeve from
beneath the eyepiece.
왘Push the graticule in mount
over the sleeve, ensuring that
it seats properly.
왘Screw the sleeve into position
again.
왘Insert the eyepiece fully into
the eyepiece tube.
왘Looking into the eyepiece, turn
the eyepiece in the eyepiece
tube to align the graticule, and
tighten the clamping screw.
The dioptric adjustment, and setting for parfocality when a
graticule is in the eyepiece, are described in the user manuals
for photomicrographic and measuring systems.
Stands
Transmitted-light stand, bright field, 20W
Follow the safety instructions given
on page 4.
왘Fit the side-faced column to the transmitted-light base as
described on page 16.
왘Fit the microscope carrier and the stereomicroscope as
described on page 16.
Fitting the bulb
Never touch the bulb when it is hot.
You may sustain burns.
• Do not touch the 6V/20W halogen bulb directly with your
fingers, because fingermarks can cause it to crack when
heated. Remove fingermarks immediately with a cloth dipped
in pure alcohol.
왘Insert the slider together with
the bulb socket.
왘Hold the new bulb with a cloth
and insert it fully into the mount.
왘Push the slider carefully but
completely back along the rail to
the stop.
Filters
• The transmitted-light stand contains a KG1 filter.
• You can insert other 50mm diameter filters if you need them:
왘Pull out the slider.
왘Insert the 50mm diameter filter.
왘Push the slider carefully but
completely back along the rail to
the stop.
Starting up
The ex-factory setting of the voltage selector is 230 (for voltages
between 200–240V) and the fuse holder contains two 160mAT fuses.
For 100–120V power supply:
왘Set the voltage selector to 115.
왘 Fit the enclosed two 315mAT fuses
in the fuse holder instead of the
two 160mAT fuses (see section
“Changing fuses”, page 21).
왘Connect the power cable to the
socket of the base and to the
power source.