Leica DMi8 A User manual

Installation Manual
Leica Microsystems CMS GmbH •Manual 11934095, Revision 1.0 from 2015-01-15
Leica DMi8 A
Leica DMi8 C
Leica DMi8 M

Coyprights
Revision 1.0, published on 2015-01-15 by:
Leica Microsystems CMS GmbH
Ernst-Leitz-Strasse 17-37
D-35578 Wetzlar, Germany
http://www.leica-microsystems.com
Responsible for contents:
Marketing CMS
Copyright
All rights to this documentation are held by Leica Micro-
systems CMS GmbH. Reproduction of text or illustrations
(in whole or in part) by print, photocopy, microfilm or other
method (including electronic systems) is not allowed with-
out express written permission from Leica Microsystems
CMS GmbH.
The instructions contained in the following documentation
reflect state-of-the-art technology. We have compiled the
texts and illustrations as accurately as possible. Still, we
are always grateful for comments and suggestions regard-
ing potential mistakes within this documentation.
The information included in this manual may be changed with-
out prior notice.
2

Contents
Contents
1. Important Notes about this Manual .......................... 4
1.1 Text symbols and their meanings............................... 4
2. Safety Notes .................................................................. 6
2.1 General safety notes .................................................... 6
2.2 Technical data ............................................................... 6
3. Overview of Components............................................ 8
4. Unpacking.................................................................... 12
5. Assembling the Microscope .................................... 14
5.1 Assembling the DIC Module
and DIC objective prisms........................................... 15
5.2 Assembling the specimen stage .............................. 16
5.3 Inserting the eyepieces ............................................. 18
5.4 Inserting the objectives ............................................. 18
5.5 Assembling the incident light illuminator................ 19
5.5.1 Assembling the LED lamp housing........................... 19
5.5.2 Assembling the fluorescence illuminator............... 19
5.6 Equipping the incident light reflector turret ........... 20
5.7 Assembling the polarizer and analyzer................... 21
5.8 Assembling the transmitted light
illuminator arm (TL)..................................................... 22
5.8.1 Tiltable transmitted light illuminator arm ................ 22
5.8.2 Fixed transmitted light illuminator arm.................... 23
5.9 Assembling the condensers
for the tiltable transmitted light illuminator arm .... 24
5.10 Assembling the transmitted light lamp housing..... 30
5.11 Wiring............................................................................ 31
5.11.1 Wiring for microscopes with
transmitted light illuminator arm .............................. 32
6. Startup .......................................................................... 33
6.1 Adjusting the diaphragms
for the incident light illuminator................................ 33
6.2 Inspecting the phase contrast ring ......................... 34
6.3 Setting the polarizer ................................................... 36
6.4 Condenser centering.................................................. 37
7. Index ............................................................................. 38
3

1. Important notes about thIs manual
1. Important Notes about this Manual
Numbers in parentheses, e.g. (Fig. 1.2), refer to illustrations,
in the example to Fig. 1, Item 2.
Numbers with an arrow, e.g. →p. 20, refer to a specific
page of the manual.
WARNING indicates a hazard with a medium degree of risk
that, if not avoided, can result in death or serious injury.
CAUTION indicates a hazard with a low degree of risk that,
if not avoided, can result in minor or moderate injury.
Caution!
In this manual, additional safety notes are indicated with
the triangle symbol shown here, and have a gray back-
ground.
Caution! The instrument and accessories can be damaged
when operated incorrectly.
Caution!
This Installation Manual is an essential component of
the product; it must be read carefully before the product
is assembled, put into operation or used, and must be
kept for later reference.
This Installation Manual includes important instructions
and information related to the operating safety and mainte-
nance of the instrument.
1.1 Text symbols and their meanings
(Fig. 1.2)
→p.20
!
WARNING
CAUTION
4

1. IMPORTANT NOTES ABOUT THIS MANUAL
Warning of hazardous electrical voltage! Danger of electric
shock!
Danger due to hot surface
Warning of optical radiation! Never look directly into the
light beam! Wear safety goggles!
Warning of electromagnetic field
Warning of UV radiation! Wear safety goggles!
Warning of hazardous laser radiation – take appropriate
safety precautions!
Warning of hand injuries
Instructions for disposing of the instrument, its acces-
sories and consumables.
Connection to ground
Explanatory note
Item not contained in all equipment configurations
Manufacturing date, e.g. 11 / 2011 for November 2011
China RoHS 50 years EFUP
(Environmentally friendly use period)
*
MM/YYYY
5

2. Safet y NoteS
2. Safety Notes
2.1 General safety notes
Caution!
Make sure to follow the safety notes in the Leica DMi8
User Manual.
Caution!
Also see the instructions and warnings contained in this
Installation Manual.
Caution!
The instruments and accessories described in this In-
stallation Manual have been safety-tested and checked
for possible hazards.
The responsible Leica representative or the main plant
in Wetzlar must be consulted whenever the device is
altered, modified or used in conjunction with non-Leica
components that are outside of the scope of this manual!
Unauthorized alterations to the instrument or noncom-
pliant use shall void all rights to any warranty claims!
2.2 Technical data
Compact Leica CTR electronics box
For indoor use only.
Supply voltage: 100 – 240 VAC
Frequency: 50 / 60 Hz
Power consumption: max. 150 VA
Fuses: 3.15 A, slow-blowing,
breaking capacity H, 250 VAC
Size: 5x20 mm
Ambient temperature: 15° - 35°C
Relative humidity: 90% up to 30°C,
non-condensing
Protection class: I
Overvoltage category: II
Pollution degree: 2
Advanced/Advanced+ Leica CTR electronics box
For indoor use only.
Supply voltage: 100 – 240 VAC
Frequency: 50 / 60 Hz
Power consumption: max. 290 VA
Fuses: 6.3 A, slow-blowing,
breaking capacity H, 250 VAC
Size: 5x20 mm
Ambient temperature: 15° - 35°C
Relative humidity: 90% up to 30°C,
non-condensing
Protection class: I
Overvoltage category: II
Pollution degree: 2
6

2. Safet y NoteS
Leica DMi8 M microscope (manual)
For indoor use only.
Supply voltage: 100 – 240 VAC
Frequency: 50 / 60 Hz
Power consumption: max. 55 VA
Fuses: 1.6 A, slow-blowing,
breaking capacity H, 250 VAC
Size: 5x20 mm
Ambient temperature: 15° - 35°C
Relative humidity: 90% up to 30°C,
non-condensing
Protection class: I
Overvoltage category: II
Pollution degree: 2
Leica DMi8 C microscope (manually encoded)
For indoor use only.
Supply voltage: 100 – 240 VAC
Frequency: 50 / 60 Hz
Power consumption: max. 55 VA
Fuses: 1.6 A, slow-blowing,
breaking capacity H, 250 VAC
Size: 5x20 mm
Ambient temperature: 15° - 35°C
Relative humidity: 90% up to 30°C,
non-condensing
Protection class: I
Overvoltage category: II
Pollution degree: 2
Leica DMi8 A microscope (automated variants)
For indoor use only.
Supply voltage: 100 – 240 VAC (→ Leica CTR)
Frequency: 50 / 60 Hz (→ Leica CTR)
Power consumption: see Leica CTR
Fuses: see Leica CTR
Ambient temperature: 15° - 35°C
Relative humidity: 90% up to 30°C,
non-condensing
Protection class: I (→ Leica CTR)
Overvoltage category: II (→ Leica CTR)
Pollution degree: 2 (→ Leica CTR)
Leica EL6000*
For indoor use only.
Supply voltage: 100 – 240 VAC
Frequency: 50 / 60 Hz
Power consumption: max. 200 VA
Fuses: 2.5 A, slow-blowing,
breaking capacity H, 250 VAC
Size: 5x20 mm
Ambient temperature: 0° - 40°C
Relative humidity: 90% up to 30°C,
non-condensing
Protection class: I
Overvoltage category: II
Pollution degree: 2
(* See provided Instructions for Use)
7

3. Overview Of COmpOnents
Fig. 1: Right side of the Leica DMi8 A
7 Aperture diaphragm
8 Field diaphragm
9 Polarizer slot
10 Analyzer slot
11 Cover for right side-port
12 Cover for motorized incident light turret disk
3. Overview of Components
1 Objective turret with objectives
2 Tube
3 Eyepieces and eyepiece tubes
4 Specimen stage
5 LED lamp housing
6 Colour filter slot
5
6
1
87
10
4
2
3
912 11
8

3. Overview Of COmpOnents
Fig. 2: Left side of the Leica DMi8 A
1 Motorized DIC objective prism disk
2 Cover plate (not used)
3 Service port
4 Left side-port with camera
1
3
4
2
9

3. Overview Of COmpOnents
Fig. 3: Left side of the Leica DMi8 C
1 Eyepieces and eyepiece tubes
2 Tube
3 Top-port with camera
4 Cover plate (not used)
5 Incident light turret disk
6 Cover for left side-port
7 Manual DIC objective prism disk
8 LED lamp housing
9 Objective turret with objectives
10 Specimen stage
1
2
4
67
8
9
10
5
3
10

3. Overview Of COmpOnents
Variants with transmitted light illuminator arm
Fig. 4: Left side of the Leica DMi8 with tiltable transmitted light illumi-
nator arm
1 Tiltable transmitted light illuminator arm
2 Condenser height adjustment
3 Manual polarizer
4 Manual condenser, alternatively: motorized condenser
5 Condenser head
6 Condenser height stop
1
3
4
2
5
6
Fig. 5: Right side of the Leica DMi8 with tiltable transmitted light
illuminator arm
1 Transmitted light filters
2 Transmitted light lamp housing receptacle
3 Condenser with motorized polarizer
Fig. 6: Left side of the Leica DMi8 with fixed transmitted light illumina-
tor arm
1 Stop lever for condenser height adjustment
2 Fixed transmitted light illuminator arm
3 Transmitted light lamp housing
4 Receptacle for transmitted light filter
5 Light ring slide
6 Manual condenser with aperture diaphragm
2
1
3
4
6
5
1
3
2
11

4. Unpacking
4. Unpacking
The microscope is delivered in several packages depend-
ing on instrument configuration and the scope of the sys-
tem.
The Leica CTR electronics box, Leica SmartMove control el-
ement, Leica STP4000/8000 control panel, movable stages/
stage accessories and Leica EL6000 compact light source
are delivered in separate packages.
Caution!
The microscope may only be set up, installed and put
into operation by authorized Leica personnel!
Caution!
The microscope may only be operated by personnel who
have been instructed by an authorized Leica employee!
Please compare the contents of the package you receive
carefully with the packing slip, the delivery note or the in-
voice. We highly recommend keeping a copy of these docu-
ments with the manual in order to have delivery date and
delivery package information available when (for example)
placing repeat orders or making service calls. Please make
sure that no small parts are lost in the packaging materials.
Some of our packaging materials have symbols to help in
environmentally responsible recycling.
First, carefully remove all components from the transporta-
tion and packaging materials.
Caution!
If an instrument or its packaging have visible damage,
do not put the instrument into operation.
Note:
If at all possible, avoid touching optical surfaces such as
the lens surfaces on the objective. If fingerprints do appear
on the glass surfaces, remove them with a soft leather or
linen cloth. Even small traces of finger perspiration can
damage the surfaces in a short time. See the chapter on
"Care of the Microscope"→ for additional instructions in
the Instructions for Use.
Caution!
Do not connect the microscope and peripherals to the
power supply at this point under any circumstances!
Installation location
Work with the microscope should be performed in a dust-
free room, which is free of oil vapors, other chemical va-
pors and extreme humidity. At the workstation, large tem-
perature fluctuations, direct sunlight and vibrations should
be avoided. These conditions can distort measurements
and longer microscopic scans.
Permitted ambient conditions:
Temperature 15–35°C
Relative humidity maximum 90% up to 30°C
non-condensing
Microscopes in warm and warm-damp climatic zones re-
quire special care in order to prevent fungus contamination.
For additional instructions, see the chapter on "Care of the
Microscope" in the→ User Manual.
Caution!
The microscope must be set up so that the power volt-
age input is not obstructed and the instrument can
quickly be removed from the power supply if necessary.
12

4. Unpacking
Caution!
Electrical components must be placed at least 10 cm
away from the wall and away from flammable substances.
Weight
The weight of the microscope depends on the respective
equipment configuration.
CAUTION
At a full load, the microscope weighs more than 18kg.
The user must take corresponding precautions for trans-
porting the instrument.
13

5. Assembling the microscope
The microscope components +) are logically assembled
in this order:
• Only for microscopes with transmitted light illuminator
arm (chapter 5.8-5.10):
• Transmittedlightilluminatorarm
• Condenserwithcondenserhead
• Transmittedlightlamphousing
• DICmoduleandDICobjectiveprisms
• Specimenstage
• Eyepieces
• Objectives
• Incidentlightilluminator(LEDlamphousing)
• Equipmentfortheincidentlightturret
• Polarizerandanalyzer
When using other systems and advanced optical accesso-
ries, the sequence may vary.
Only a few commonly used screwdrivers and keys are
necessary for assembly; these are included in the delivery
package.
5. Assembling the Microscope
+) Depending on scope of delivery
14

5. Assembling the microscope – Dic moDule AnD Dic objective prisms
5.1 Assembling the DIC Module and DIC objective
prisms
If the microscope is not equipped with DIC, please jump
ahead to Chapter 5.2.
In microscopes of the Leica DMi8 M series, the DIC prisms
are already installed in the DIC disk (Fig. 7) below the objec-
tive turret. Motorized, manually encoded and manual DIC
disks are offered. Assembly is identical for all variants.
When upgrading the IC prism disk, follow these steps:
• Removethefrontpanel(Fig.8)undertheobjectiveturret
after loosening the Allen screws (Fig. 9).
• Insert the DIC prism disk (Fig. 7) evenly into the recep-
tacle. Start by gently turning one screw using the 3 mm
hexagonal key provided. Then, tighten both Allen screws.
!Caution!
Insert the prism disk so that the electronics board is fac-
ing downwards. Do not touch the electronics (especially
the contacts) with your fingers!
Upgrading individual IC prisms:
• LoosenbothAllenscrewsandremovetheprismdisk.
• Placethe prismsagainstthe peg(Fig.7.3) andthe shim
between the prism and screw (Fig. 10) and lightly tighten
to avoid tension. Insert the prisms so that the identifying
letter, or ID, is facing upwards and is legible.
• Afterassemblingtheprisms,reinserttheprismdiskinto
the receptacle.
Alternatively:
Insert the objective prism slide (Fig. 11) into the slot below
the objective turret. The code letter must match the code
letter on the objective.
Fig. 7: DIC objective prism turret (encoded and motorized)
1 IC objective prism in mount
2 Identifying letter (ID)
3 Orientation pin
3 2 1
Fig. 8: Front panel for DIC prism disk
Fig. 9: Removing the front panel
Fig. 10: IC objective prism
1 Objective prism in mount
2 Washer and screw
21
Fig. 11: Objective prism slide
15

5. Assembling the microscope – specimen stAges
5.2 Assembling the specimen stage
There is a wide variety of available specimen stages.
The accompanying figures depict a few examples.
The assembly of each of these stages is identical. The
stages are permanently affixed to the microscope using 3
screws (Fig. 14). In the case of fixed stages, a mechanical
stage can be attached (Fig. 15).
!Caution!
Screw lengths may vary. The following applies to the ship-
ment of different screws: Always use the shorter of the
3 screws for the front hole and both screws of equal length
for the rear holes.
• Alignthestagesothatbothholespointbacktowardsthe
illumination axis and the single hole is facing in the direc-
tion of the tube.
• Align the fastening holes in the stage with the holes in
the contact surface. If the holes are not visible, as in the
case of the 3-plated cross-stage or scanning stage, gen-
tly move the upper tabletop until the opening is visible.
• Startbygentlyturningthefrontscrewusingtheprovided
3 mm hexagonal key. It is important that the shorter of the
3 screws is always placed in the front hole, because a
screw that is too long can damage the focus hub.
• Then,tightenbothrearscrews.
• Finally,retightenthefrontscrew.
Fig. 12: Fixed stage
Fig. 13: Mechanical 3-plate stage
Fig. 14: a, b assembly screws for 3-plate cross-stage
a b
16

5. Assembling the microscope – specimen stAges
Fixed stage
Mechanical stages for fixing holders for various culture
flasks are also optionally available for fixed stage plates
(Fig. 15).
• The mechanical stage has 2 screws. Tighten these
screws into the threaded holes on the bottom of the fixed
stages using the 3 mm hexagon socket wrench and re-
tighten regularly after frequent use of the mechanical
stage.
The mechanical stage is preadjusted at the factory. If the
mechanical stage shifts out of focus when moving from
right to left, this can be corrected by Leica Technical Ser-
vice.
• Now,removetheorderedstageinsertorinserts(Fig.16)
from the packaging and guide one stage insert into the
precise click system. The stage, mechanical stage and
stage insert are now ready to use.
Self-adhesive scales for reading the coordinate adjustment
are included with some (not all) inserts.
• Finally,afxthesescalesintherecessesofthemechani-
cal stage.
Motorized 3-plate or scanning stage
3-plate stages and scanning stages:
• Afterassemblingthestage,rstconnect(inthecaseof
motorized stages) the provided stage cable to the socket
on the stage and then to the electronics box. The corre-
sponding mark on the box is called "XY- Stage".
Various inserts are offered with the normal 3-plate or scan-
ning stages (including heated inserts).
• Carefully place these inserts into the corner provided
with spring clips from above at an angle. The stage in-
serts click into place once positioned correctly.
!Caution!
Only press on the spring clips from the sides.
Never press on the insert plate at the spring clips from
above at an angle, because then the insert will not be
plane-parallel to the stage and cannot be bent.
Fig. 15: Mechanical stage for fixed micromanipulation stage
Fig. 16: Inserts for mechanical stage (fixed stage)
For additional examples, see special brochure
17

5. Assembling the microscope – eyepiece And objectives
5.3 Inserting the eyepieces
• Inserttheeyepiecesintotheeyepiecetubes.
Note:
We recommend using the LAS software to teach in eye-
pieces that are not included in the scope of delivery.
This ensures that the total magnification specified on the
touchscreen is correct.
See LAS software manual:
Fig. 17: Eyepieces
5.4 Inserting the objectives
• Screwtheobjectivesintotheobjectiveturret.
The receptacles on the objective turret are numbered
(Fig. 18). Depending on your equipment, specific positions
have already been assigned to the individual objectives at
the factory.
For details on the exact positions of the objectives,
please refer to the enclosed "Identification sheet".
!Caution!
Close vacant threads in the turret using dust protection
caps!
Please note that the front lenses of the objectives face up-
wards. This subjects them to increased exposure to con-
tamination compared to objectives on upright microscopes.
Check the front lens more often for cleanliness for this rea-
son.
Note:
We recommend carrying out a parfocality adjustment via
the LAS software.
See LAS software manual:
Fig. 18: Objective turret
Fig. 19: Objective turret, equipped
18

5. Assembling the microscope – illuminAtion
5.5 Assembling the incident light illuminator
Caution!
Ensure that the lamp housing has been disconnected
from the power supply. Unplug the power plug and the
power supply during assembly.
CAUTION
Light sources pose a potential irradiation risk (blinding,
UV radiation, IR radiation). Therefore, lamps may only be
operated in closed housing and in an installed condition.
Never look directly into the beam path (blinding hazard).
5.5.1 Assembling the LED lamp housing
• Placethelamphousingintotheincidentlightlamphous-
ing receptacle (Fig. 20) and fasten it with the side clamp-
ing screw.
Leica DMi8 M with manual incident light method:
• Connect the cable of the lamp housing to the port (Fig.
21.1) on the rear side of the microscope.
Leica DMi8 A and C with motorized incident light method:
• Connectthecableofthelamphousingtothepowersup-
ply for transmitted light for the Leica CTR electronics box
("Lamp RL" port).
1
Fig. 20: Fastening the lamp housing to the incident light axis
1 Fastening screw
5.5.2 Assembling the fluorescence illuminator
Leica EL6000
See separate manual.
CAUTION
When using the Leica EL6000 compact light source, it is
essential to observe the safety notes in the separate in-
structions included.
CAUTION
Just switch on the light source once the light guide is
connected to the microscope.
Uncontrolled light output from the light guide poses a
blinding hazard!
There is also a burn hazard at the light guide output!
• ScrewtheLeicaEL6000microscopeadapterontotherear
side of the microscope in the lamp housing receptacle.
• Closethelightguideaccordingtotheinformationinthe
Leica EL6000 manual.
Fig. 21: Manually connecting the lamp housing for the Leica DMi8
1 Incident light lamp housing connection
1
19

5. Assembling the microscope – incident light turre t equipment
5.6 Equipping the incident light reflector turret
!Caution!
First, completely read through this section before you begin
equipping the reflector turret disk.
The reflector turret disk can be accessed from both the
right side and the left side of the microscope (Fig. 22).
The positions on the reflector turret disk are numbered. De-
pending on how they are equipped, the individual filter and/
or reflector cubes are set in pre-assigned positions at the
factory. For details, check the identification sheet included
with your order.
•Removethecoverfromthereectorturretdisk(Fig.22.1).
The cover is secured in place by a magnetic holder and
can be detached from the front.
• Withtheholderfacingyousquarely,slidetheltercube
or reflector cube forwards into the holder as described in
the identification sheet provided.
•Checktoensurethatthecubeisplacedproperly.Aloose
cube can be damaged when rotating the turret disk or
can block the disk.
• Oncealllterblocksand reectorblockshavebeenin-
serted, put the cover back in place.
Note:
Is the microscope equipped with a 2-fold filter turret, the
turret must be pushed across the arrester position to ex-
change the filter cubes. It is not possible to insert the filter
cubes when the turret is in the arresting position.
1
Fig. 22: Access to turret disk
1 Cover
Fig. 23: Equipping the turret disk
Fig. 24: Turret disk with used filter cubes
20