Leica ES2 User manual

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Leica ES2
Leica EZ4
Leica EZ4 HD
Manual

Leica E Series Manual 2
Congratulations!
You have just purchased a Leica E Series stereo-
microscope - an excellent choice! The Leica E
series is perfectly suited for practical instruc-
tion in schools and universities as well as for
routine tasks in the laboratory and industrial
production and testing. Viewing entire objects
under magnication imparts perspectives and
discoveries that would not be possible with the
naked eye.
In developing our stereomicroscopes, we have
placed great emphasis on simple, self-explana-
tory operation. However, please take the time
to read the instruction manual and the notes on
operating safety, to learn about all the features
and capabilities of your instrument so that
you can use it safely and to its best advantage.
Should you have any questions, please consult
your local Leica representative. We are gladly at
your service!

Leica E Series Manual 3
Chapter Overview
Safety and Use 6
Leica ES2 10
Leica EZ4 and Leica EZ4 HD 18
Eyepieces (only for Leica EZ4) 29
Photography Using the Leica EZ4 HD 37
The Camera Menu 51
Care and Transport 62
Specications 64
Dimensions 67

Leica E Series Manual 4
Leica ES2
Overview: Leica ES2 11
Transport, Placement and Storage 12
The Correct Interpupillary Distance 13
Using the LED illuminator 14
Focusing 15
Magnication Display 16
Adjusting the Resistance of the Focus Drive 17
Leica EZ4 and Leica EZ4 HD
Overview: Leica EZ4 19
Instrument variant: Leica EZ4 HD 20
Transport, Placement and Storage 21
The Correct Interpupillary Distance 22
Switching on the LED Illuminator 23
Controlling the Automatic Shuto 24
Illumination types 25
Focusing 26
Magnication Display 27
Adjusting the Resistance of the Focus Drive 28
Table of Contents
Eyepieces (only for Leica EZ4)
Changing Eyepieces (Only for Leica EZ4, Open Version) 30
Eyepieces and Eyecups (Only for Leica EZ4, Open Version) 31
Dioptric Correction (Only for Leica EZ4, Open Version) 32
Graticules (Only for Leica EZ4, Open Version) 34
Graticules: Preparation 35
Inserting the Graticules 36
Photography Using the Leica EZ4 HD
Leica EZ4 HD: Overview of the instrument 38
General Instructions 39
Introduction 40
Standard delivery 41
HD mode and PC mode 42
Capturing Images without a Computer in HD Mode 43
High-resolution Video Display without a Computer (HD mode) 45
PC Mode: Use with a Computer 46
PC Mode: Limitations 47
PC Mode: Additional Use of a HDMI Device 48
Conguring the White Balance 49
Restoring Factory Settings 50

Leica E Series Manual 5
Contents (Continued)
The Camera Menu
Optional Remote Control 52
Viewing Images and Movies with the Optional Remote Control 53
Calling up the Camera Menu 54
COLOR (Automatic White Balance) 55
COLOR (Manual White Balance) 56
EXPOSURE 57
RESOLUTION 58
SETUP CAMERA (Camera Settings) 59
SETUP USER (User-dened Settings) 60
Predened Illumination Scenarios 61
Care and Transport
Care and Transport 63
Specications
Camera Module 65
Dimensions
Leica ES2 68
Leica EZ4 HD 70
Leica EZ4, 10× Eyepieces 72
Leica EZ4 without Eyepieces 74

Leica E Series Manual 6
Safety and Use

Leica E Series Manual 7
Before installing, operating or using the instrument, it is mandatory to read this
user manual. In particular, please observe all
safety instructions.
The "Safety Concept" brochure contains additi-
onal safety information regarding service work,
requirements and the handling of the micro-
scope, accessories as well as general safety inst-
ructions.
You can combine individual system articles with
articles from external suppliers. Please read the
user manual and the safety requirements of the
supplier.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Warning of a danger
This symbol indicates especially impor- tant information that is mandatory to
read and observe. Failure to follow these inst-
ructions may pose hazards to personnel, cause
instrument malfunctions or damage the instru-
ment.
Hazardous electrical voltage
This symbol indicates especially impor- tant information that is mandatory to
read and observe. Failure to follow these inst-
ructions may pose hazards to personnel, cause
instrument malfunctions or damage the instru-
ment.
Important information
This symbol indicates additional infor- mation or explanations that intend to
provide clarity.
EC Declaration of Conformity
Electrically operated accessories are construc-
ted based on the state of the art of technology
and are provided with an EC Declaration of
Conformity.
Safety Notes
Contact address
Leica Microsystems (Switzerland) Ltd.
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)

Leica E Series Manual 8
Intended use
The E Series microscopes are optical instru-
ments for improving the visibility of objects
through magnication and illumination. It is
used for observing and documenting.
Place of use
Use the instrument in enclosed,
dust-free rooms at +10 °C to
+40 °C only. Protect it from oil, chemicals and
extreme humidity. It must be at least 10 cm
from the wall and away from ammable subs-
tances.
Interference
Avoid large temperature uctuations, direct
sunlight and vibrations. These conditions
can distort measurements and micrographic
images.
In warm and warm-damp climatic zones, the individual components require
special care in order to prevent the build-up of
fungus.
Use in clean rooms
Leica E Series microscopes can be used in clean
rooms without any problems.
Handling electrical components
Never install any other plug (NEMA 5-15P) or unscrew any mechanical
components unless expressly instructed to do
so in the instructions.
The microscope must be connected to a grounded outlet.
Always position the microscope so that you can disconnect it from the power
supply at any time. The power cable is provided
as the power disconnect device.
Instructions for Use

Leica E Series Manual 9
Intervention from the user
The microscopes of the E Series contain no components that can be maintained
or repaired by the user. Exceptions are menti-
oned specically in this manual.
Unless otherwise specied in this manual, repair and service work may
be carried out by authorized Leica technicians
only.
Unauthorized alterations to the instru- ment or noncompliant use shall void all
rights to any warranty claims.
Maintenance
Leica E Series microscopes are basically maintenance-free. To ensure that it
always operates safely and reliably, we recom-
mend that you take the precaution of contac-
ting the responsible service organization. Here
you can arrange for periodic inspections and
conclude a maintenance contract (recommen-
ded).
Accessories
Only those accessories may be used that are
listed in this user manual or for which safe use
has been conrmed by Leica Microsystems.
Danger of infection
Direct contact with eyepieces is a poten- tial transmission method for bacterial
and viral infections of the eye. The risk can be
kept to a minimum by using personal eyepieces
for each individual or detachable eyecups.
Instructions for Use (Continued)

Leica E Series Manual 10
Leica ES2

Leica E Series Manual 11
Overview: Leica ES2
1
2
3
5
7
6
4
1 10× xed eyepieces for eyeglass wearers
2 Carrying handle
3 Two-level magnication changer 10× and 30×
4 Focusing drive
5 Integrated LED incident light illumination
6 Integrated LED transmitted illumination
7 Switch for incident and transmitted light

Leica E Series Manual 12
Built-in carrying device
All Leica E Series microscopes are equip- ped with a carrying handle for conveni-
ent and safe transport.
Setting up the microscope
1. Place the microscope on a at tabletop.
2. Connect the power supply to a grounded
socket.
Transport, Placement and Storage
Packing the device away
1. After use, wind up the cable as shown
in the illustration.
2. Store the instrument so that it is protected
against dust.

Leica E Series Manual 13
Setting the eye distance
1. Look into the eyepieces.
2. Hold the eyepieces with both hands. Push
the eyepieces together or separate them
until you see a circular image.
Note for eyeglass wearers
If you wear glasses, fold the eyecups back – otherwise, fold the eyecups forwards.
The Correct Interpupillary Distance
The Ideal Image
The interpupillary distance is correctly set if you see a single circular image eld
when looking at a specimen.
You may need a short time to become familiar
with using the instrument. Not to worry—after
a little while, it will become automatic.
✓
✗

Leica E Series Manual 14
Iluminating the specimen
1. Position a specimen in the middle of the
baseplate.
2. Switch the two LED illuminators on or o as
desired.
After switching o, the LEDs remain lit for about another 4 seconds.
Tips on use
ϘUse incident light for plastic, opaque
objects.
ϘUse transmitted light for transmitted light
specimens or transparent objects.
ϘUse the combination of both LED illumina-
tors for partially transparent objects.
Using the LED illuminator

Leica E Series Manual 15
Focusing raises and lowers the stereomi- croscope using the focusing drive. The
specimen is brought into sharp focus as soon as
it is in the focal point of the objective.
The focusing drive can be operated either left-
or right-handed.
Focusing
1. Set the magnication changer to the lowest
level (10).
2. Set the working distance to approx.
100mm for coarse focusing.
3. Set the magnication changer to the
second level (30).
4. Use the focusing drive once again for ne
focus.
After you have adjusted the image sharp- ness at high magnication, it is maintai-
ned even if you switch to a lower magnication
(parfocal).
Focusing
Focusing drive torque

Leica E Series Manual 16
Magnication Display
You can read the congured magnica- tion on the right-hand rotary knob of the
Leica ES2. The additional magnication provi-
ded by the eyepieces is already incorporated in
the scale.

Leica E Series Manual 17
Adjusting the Resistance of the Focus Drive
Adjusting the resistance
Is the focusing drive too easy or too dicult
to adjust? No problem - the resistance can be
adjusted individually depending on your perso-
nal preferences:
1. Grip the drive knobs with both hands and
turn them towards each other until the
desired resistance is reached during focu-
sing.

Leica E Series Manual 18
Leica EZ4 and Leica EZ4 HD

Leica E Series Manual 19
Overview: Leica EZ4
1
2
3
5
7
6
4
1 10x xed eyepieces for eyeglass wearers
2 Carrying handle
3 Magnication changer
4 Focusing drive
5 Integrated LED incident light illumination
6 Illumination control
7 Integrated LED transmitted illumination

Leica E Series Manual 20
Instrument variant: Leica EZ4 HD
1
2
3
5
6
9
8
7
4
1 10x xed eyepieces for eyeglass wearers
2 Integrated digital camera with SD card slot
3 Carrying handle
4 Magnication changer
5 IR receiver for optional remote control
6 Focusing drive
7 Integrated LED incident light illumination
8 Illumination control
9 Integrated LED transmitted illumination