Leica DMI6000 B User manual

DMI6000 instructions
The screen is not tactile!
On the right!!!
Fribourg, March 2009 Author: Sylvie DUCREUX
Your attention please: this manual is only a light version of the complete DMI6000
operating manual from Leica, if you need more details, please feel free to read it, one
copy is located inside our cupboard or ask me for the pdf file.

DMI6000 instructions
March 2009
Created by Sylvie Ducreux Page 2
Motorized platform = Z stage
SmartMove = the remote control element
Function keys on the right side of the stand
Z ↑to set the focus position, press at the same time Z↑
SET and SET (idem if you want to clear the previous set
Z ↓focus)
Light Adjustments
Defined function keys
1. Move in x-direction
2. Move in y-direction
3. Focus (= Z direction; but you’re free to use the regular wheel)
4. Height adjustment (you must not play with it, thanks!)
5. Variable function keys: Left XY fine Right Z fine
XY fast Z coarse
1. TL/IL: transmitted light/ incident light
2. AP: Aperture diaphragm
3. Light intensity (free increase with TL but fixed
step in IL= 10%, 17%, 30%, 55%, 100%)
4. Field diaphragm

DMI6000 instructions
March 2009
Created by Sylvie Ducreux Page 3
Objectives (see Special Notes)
You can exchange objective either by
1) pressing the bottom button to exchange between
DRY/IMMERSION then use the button or to
rotate the obejective turret to higher or smaller
magnification
Unlike the DM6000 screen, the DMI6000 Leicascreen is NOT tactile!!!
1)
2) or directly turning the objective turret NOT RECOMMENDED!!!!
Avoid switching manually the objectives, as you may inadvertently rotate the objective
ring, or even worse, the objective itself.
3) Third alternative, you can also exchange objectives automatically inside the LAS-AF
software in the “Acquire” tab
Dry = x10
Immersion = x20 (water/glycerol with a special knurled ring) can be also use with
oil but need to be carefully cleaned after!
x63 blue ring for water immersion + additional knurled ring
x63 silver ring for glycerol immersion+ additional knurled ring
Notes:
The x63 objectives are common for both microscopes!
Meaning that if one is missing on one side, it must be on the other microscope, just unscrew it and
exchange it with the black plastic cache from the other side, don’t let the empty position open
exposed to dust!
Water and 80% are provided for common use and placed in the cupboard.
Nevertheless, please, don’t let stocks empty without informing the responsible person
Objectives must be cleaned after use…
!!! Attention!!! Inverted orientation carefully clean objective, revolver platform, especially if you
had some leakage

DMI6000 instructions
March 2009
Created by Sylvie Ducreux Page 4
Fluorescence = 2 cubes
N2.1
I3
Additional cubes are available
A
CFP
YFP
L5
Cubes can be exchanged by opening the drawer on the microscope right side.
If you don’t know how to do it, don’t try!
Ask for help!
(or see detailed protocol in the DMI6000 operating
manual)