Leica DM Series User manual
Leica DM 750 Manual 3
Contents
Safety regulations 4
Safety concept 5
Symbols used in this operating manual 6
Important Notes 7
Instructions on use 8
Health risks and dangers of use 10
Information for the person responsible
for the instrument 11
Care instructions 12
Accessories, maintenance and repair 13
Electrical data and ambient conditions 14
The Leica DM 750 15
Introduction 16
Unpacking 17
Get Ready! 18
Substage Illumination 19
Attaching the viewing Tubes 20
Leica EZ Viewing Tube –
integrated eyepieces 21
Leica Standard Viewing Tube –
separated eyepieces 23
Eyeguards 24
Installing Objectives 25
Installing the Substage Condenser 26
Get Set! 28
Turning on the microscope 29
Complete Condenser Centration 30
Using the Condenser 32
Prepare to View a Specimen Slide 33
Focusing 34
Viewing Tube Adjustment 35
Koehler Setup 37
Koehler Setup (cont.) 38
Oil Immersion Technique 39
Time Delay Shutoff 41
Go! 42
Ready! Set! Go! 43
Care of the Microscope 44
General maintenance 45
Dimensions 47
Leica DM 750 Manual 5
Safety concept
The individual modules of the Leica DM micros-
copy series include an interactive CD-ROM with
all relevant user manuals several languages.
Keep it in a safe place, and readily accessible
to the user. User manuals and updates are also
available for you to download and print from
our web site at
www.leica-microsystems.com.
This operating manual describes the special
functions of the individual modules of the Leica
DM microscopy series and contains important
instructions for their operational safety, main-
tenance, and accessories.
The "Safety concept" booklet contains addi-
tional safety information regarding the service
work, requirements and the handling of micro-
scope, accessories and electrical accessories as
well as general safety instructions.
You can combine individual system articles with
articles from external suppliers. Please read the
user manual and the safety requirements of the
supplier.
Before installing, operating or using the instru-
ments, read the user manuals listed above. In
particular, please observe all safety instruc-
tions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Leica DM 750 Manual 6
Symbols used in this operating manual
Warning of a danger
• This symbol indicates especially impor-
tant information that must be read and
complied with.
Failure to comply can cause the following:
Hazards to personnelϘ
Functional disturbances or damaged instru-Ϙ
ments
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
Hazards to personnelϘ
Functional disturbances or damagedϘ
instruments
Danger due to hot surface.
This symbol warns against touching
accessible hot surfaces, e.g. those of
light bulbs.
Important information
This symbol indicates additional infor-
mation or explanations that intend to
provide clarity.
Explanatory notes
This symbol within the text points to addi-ϐ
tional information and explanations.
Leica DM 750 Manual 7
Important Notes
Description
The Leica DM 750 microscope meets today's
state of the art of technology. Nevertheless,
hazards may still arise during operation. The
potential risks are described below.
• Before installing, operating or using the
instruments, it is required to read this
user manual. In particular, please observe all
safety instructions.
Contact address
Leica Microsystems (Switzerland) Ltd.
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)
User Manual
This user manual includes important instruc-
tions related to operating safety, maintenance
and accessories,
Your Leica DM 750 microscope comes
with an interactive CD-ROM with all
relevant user manuals. Keep it in a safe place,
and readily accessible to the user. User manu-
als and updates are also available for you
to download and print from our web site at
www.leica-microsystems.com
Accessories from third-party suppliers
You can combine individual system articles with
articles from external suppliers. Please read the
user manual and the safety requirements of the
supplier.
Original condition
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Legal requirements
Adhere to general and local regulations relat-
ing to accident prevention and environmental
protection.
EC Declaration of Conformity
Electricallyoperatedaccessoriesareconstructed
based on the state of the art of technology and
are provided with an EC Declaration of Confor-
mity.
Leica DM 750 Manual 8
Instructions on use
Intended use
The Leica DM 750 microscope is an optical
instrument which uses magnification and illu-
mination to improve the visibility of objects. It
is used for observing and documenting.
The Leica DM 750 microscope may be used only
in closed rooms and must be placed on a solid
floor.
The Leica DM 750 microscope can be used in
clean rooms without any problems.
• Position the Leica DM 750 microscope
so that it can be disconnected from
the power supply at any time. The power cord
must remain accessible at all times, because the
power supply is foreseen as mains disconnect-
ing device.
Place of use
Only use the instruments in closed, dust free
rooms and between +10 °C and +40°C. Protect
the devices from oil, chemicals and extreme
humidity. If using the devices outdoors, protect
them from dust and moisture. Never use electri-
cal devices outdoors. Install electrical devices at
least 10 cm from the wall and away from flam-
mable substances.
Avoid large temperature fluctuations, direct
sunlight and vibrations. These conditions
can distort measurements and micrographic
images.
In warm and warm-damp climatic zones,
the individual components require
special care in order to prevent the build-up of
fungus.
Non-intended use
• Using the instrument in any way
contrary to the specifications in the user
manual can lead to bodily harm and damage to
objects. Doing so can impair the functions of
the protective measures provided.
Never install any other plug (NEMA
5-15P) or unscrew any mechanical
components unless expressly instructed to do
so in the instructions.
The devices and accessories described in
this operating manual have been tested
for safety and potential hazards.
Leica DM 750 Manual 9
Instructions on use (continued)
• The responsible Leica affiliate must be
consulted whenever the instrument is
altered, modified or used in conjunction with
non-Leica components that are outside of the
scope of this manual.
• Unauthorized alterations to the device
or noncompliant use shall void all rights
to any warranty claims.
Transport
If at all possible, use the original packaging for
shipping or transporting individual modules.
In order to prevent damage from vibrations,
disassemble all moving parts that (according to
the user manual) can be assembled and disas-
sembled by the customer and pack them sepa-
rately.
Disposal
Disposal must comply with locally applicable
laws and regulations.
Integration in third-party products
When installing Leica products into third-party
products, the manufacturer of the complete
system or its dealer is responsible for follow-
ing all applicable safety instructions, laws and
guidelines.
Leica DM 750 Manual 10
Health risks and dangers of use
Health risks
Workplaces with microscopes facilitate
and improve the viewing task, but they
also impose high demands on the eyes and
holding muscles of the user. Depending on the
duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
this reason, appropriate measures for reduction
of the workload must be taken:
Optimum workplace layoutϘ
Frequent changes of activityϘ
Thorough training of the personnel, givingϘ
consideration to ergonomic and organiza-
tional aspects
The ergonomic design and construction of the
Leica microscopy series are intended to reduce
the exertion of the user to a minimum.
Danger of infection
• Direct contact with eyepieces is a poten-
tial transmission method for bacterial
and viral infections of the eye.
The risk can be kept to a minimum by
using personal eyepieces for each indi-
vidual or detachable eyecups.
Dangers during use
The Leica DM 750 microscope may only beϘ
connected to a grounded socket.
The Leica DM 750 microscope may not beϘ
operated unless it is in proper functioning
condition.
LED Klasse 1 /LED 1 Laser Product
The illumination meets laser class 1 require-
ments in accordance with IEC 60825-1 "Safety
of LED products".
LED class 1
Class 1
LED Product
Leica DM 750 Manual 11
Information for the person responsible for the instrument
Information for the person responsible for
the instrument
Ensure that the Leica DM 750 microscope isϘ
used only by qualified personnel.
Ensure that this user manual is always avail-Ϙ
able at the Leica DM 750 microscope.
Carry out regular inspections to makeϘ
certain that the authorized users are adher-
ing to safety requirements.
When instructing new users, do so thor-Ϙ
oughly and explain the meanings of the
warning signs and messages.
Assign individual responsibilities for start-Ϙ
ing, operating and servicing the instru-
ment and monitor the observance of these
responsibilities.
Do not use the Leica DM 750 microscopeϘ
unless it is in perfect condition.
Inform your Leica representative or LeicaϘ
Microsystems (Schweiz) AG, Industry
Division, 9435 Heerbrugg, Switzerland,
immediately of any product defect that
could potentially cause injury or harm.
If you use accessories made by third-partyϘ
manufacturers with the Leica DM 750 micro-
scope, be sure that each such manufacturer
confirms the safety-engineering, harmless
usability of the product and observe the
product's user manual.
Modifications and maintenance of the LeicaϘ
DM 750 microscope may only be performed
by professionals expressly authorized by
Leica.
Only original Leica replacement parts mayϘ
be used in servicing the product.
After service work or technical modifica-Ϙ
tions, the unit must be reconfigured with
observance to our technical requirements.
If the unit is modified or serviced by unau-Ϙ
thorized persons, is improperly maintained
(as long as maintenance was not carried
out by us), or is handled improperly, Leica
will not accept any liability.
The electric installation in the buildingϘ
must conform to the national standard, e.g.
current-operated ground leakage protec-
tion (fault-current protection) is suggested.
Leica DM 750 Manual 12
Care instructions
General instructions
Protect the Leica DM 750 microscopeϘ
against damp, vapors, acids, alkalis, and
corrosive substances. Do not store chemi-
cals in the vicinity.
Protect the Leica DM 750 microscope fromϘ
oil and grease. Never grease or oil mechani-
cal parts or sliding surfaces.
Follow the instructions of the disinfectantϘ
manufacturer.
It is advisable to enter a service agreementϘ
with Leica Service.
Cleaning coated parts and plastic parts
Dust and dirt particles should be removedϘ
with a soft brush or lint-free cotton cloth.
Remove coarse debris with a moistenedϘ
disposable cloth.
Clinging dirt can be removed using petro-Ϙ
leum ether or alcohol
Acetone, xylene or nitro-containing thin-Ϙ
ners must NOT be used.
Never use chemicals to clean coloredϘ
surfaces or accessories with rubberized
parts. This could damage the surfaces,
and specimens could be contaminated by
abraded particles.
Cleaning glass surfaces
Remove dust using a fine, dry and grease-Ϙ
free brush made from hair, by blowing with
a squeeze blower, or by using a vacuum.
Remove dirt using a clean cloth that hasϘ
been moistened with distilled water.
Remove tough dirt using alcohol, chloro-Ϙ
form or petroleum ether.
Leica DM 750 Manual 13
Accessories, maintenance and repair
Accessories
Only the following accessories may be used
with the Leica DM 750 microscope:
The Leica accessories described in this userϘ
manual.
Other accessories, provided that these haveϘ
been expressly approved by Leica as being
technically safe in this context.
Maintenance
The Leica DM 750 microscope is basicallyϘ
maintenance-free. To ensure that it always
operates safely and reliably, we recommend
that you take the precaution of contacting
the responsible service organization.
You can arrange for periodic inspections
or, if appropriate, conclude a mainte-
nance contract with them.
It is advisable to enter a service agreementϘ
with Leica Service.
For maintenance and repair, only OEM spareϘ
parts may be used.
Repairs and service work
Only original Leica Microsystems spareϘ
parts may be used.
Before opening the instruments, switch offϘ
the power and unplug the power cable.
Avoid contact with powered electricalϘ
circuits, which can lead to injury.
Service address
In case of problems, please contact us as
follows:
Leica Microsystems (Switzerland) Ltd.
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)
Phone +41 71 726 33 28
Leica DM 750 Manual 14
Electrical data and ambient conditions
Environment
Temperature for use +10 °C … +40 °C
Storage temperature -20 °C … +52 °C
+50 °F … +104 °F
Manipulation shock 25 mm on 50 mm hard wood
Transport shock (unpacked) 100 g / 6 ms
Transport shock (packed) 800 mm free fall
Transport vibrations (unpacked) 5–200 Hz / 1.5 g
Atmospheric pressure "in use" and for storage: 500–1060 mBar
Humidity "in use" and for storage: 20–90%
Installation Category II (Overvoltage Category)
Pollution degree 2
Fuse Replacement
Unplug the instrument before changing any fuses. The
Leica DM 750 has two fuses, which are located behind the
power cord receptacle.
• Use only fuse type: 5×20 mm, 3.15 Amp/250V, Quick
acting (# 13RFAG300002)
Electrical data
Input: 100-240 V, 50/60 Hz, 3 W
Camera module, USB or external power supply
Input: 100-240 VAC, 50/60Hz, 0.5A
Output: 5 VDC (SELV), 2.4 A, 12 W max.
Leica DM 750 Manual 16
Thank you for purchasing the Leica DM 750
Compound Microscope from Leica Microsys-
tems. This model’s exclusive design features and
full range of accessories make it a truly versatile,
high quality-instrument for educational and
routine clinical applications.
• This instrument should only be used as
described. Hazards may result if used
improperly.
Introduction
Leica DM 750 Manual 17
Carefully remove the microscope and anyϘ
components from the packing carton.
Check the components against the plannedϘ
configuration.
Optional items such as contrast accessories,Ϙ
camera adapters, cameras, and carrying
cases are not shipped as part of the stan-
dard equipment. These items are delivered
in separate packages.
Please do not discard any of the packingϘ
materials. They should be used for safely
storing and transporting the instrument
should the need arise.
Unpacking
Leica DM 750 Manual 19
Substage Illumination
The Leica DM 750 is available with two
types of Substage Illumination. Identify
which type of Illumination you have, as this will
be important to know later.
Type 1: Standard Illumination
Adjustable condenser centration with provided
tool.
Type 2: Koehler Illumination
Adjustable condenser centration with thumb-
screws and adjustable Koehler field diaphragm.
Substage Illumination
Leica DM 750 Manual 20
Tools used
Allen keyϘThere are two types of viewing tube.
Identify which viewing tube you have:
Type 1: Leica EZ viewing tube with integrated
eyepieces.
Type 2: Standard viewing tube with separate
eyepieces
1. Slightly unscrew the set screw (located on
top of the stand) with the Allen key tool
provided.
2. Insert the dovetail into the stand support
and tighten the set screw firmly without
forcing it. This precisely positions the view-
ing tube onto the optical axis of the micro-
scope, regardless of the selected rotation.
Attaching the viewing Tubes
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