Leica M125 C User manual

Leica M125 C
Leica M165 C
Leica M205 C
Leica M205 A
User Manual

Leica M Series User Manual 2
General Instructions
Safety concept
Before using your microscope for the first
time, please read the "Safety concept" booklet
included with your instrument. It contains addi-
tional information about handling and care.
Use in clean rooms
The Leica M series can be used in clean rooms
without any problems.
Cleaning
ODo not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.
ONever use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces,
and specimens could be contaminated by
abraded particles.
OIn most cases, we can provide special solu-
tions on request. Some products can be
modified, and we can offer other accesso-
ries for use in clean rooms.
Servicing
ORepairs may only be carried out by Leica
Microsystems-trained service technicians.
Only original Leica Microsystems spare
parts may be used.
Responsibilities of person in charge of
instrument
OEnsure that the Leica stereo microscope
is operated, maintained and repaired by
authorized and trained personnel only.

Leica M Series User Manual 3
Safety Concept
The individual modules of the Leica M stereo
microscopy series include an interactive
CD-ROM with all relevant user manuals in 20
other languages. Keep it in a safe place, and
readily accessible to the user. User manu-
als and updates are also available for you
to download and print from our web site at
www.stereomicroscopy.com.
This operating manual describes the special
functions of the individual modules of the Leica
M stereo microscopy series and contains impor-
tant instructions for their operational safety,
maintenance, and accessories.
The "Safety concept" booklet contains addi-
tional safety information regarding the service
work, requirements and the handling of stereo
microscope, accessories and electrical accesso-
ries as well as general safety instructions.
You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the user manual
and the safety requirements of the supplier.
Before installing, operating or using the instru-
ments, read the user manuals listed above. In
particular, please observe all safety instructions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.

Leica M Series User Manual 4
Symbols Used
Warning of a danger
This symbol indicates especially impor-
tant information that must be read and
complied with. Failure to comply can cause the
following:
OHazards to personnel
OFunctional disturbances or damaged
instruments
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that, if not observed,
can cause the following:
OHazards to personnel
OFunctional disturbances or damaged
instruments
Danger due to hot surface
This symbol warns against touching hot
surfaces, e.g. those of light bulbs.
Important information
This symbol indicates additional infor-
mation or explanations that intend to
provide clarity.
Action
OThis symbol refers to actions described in
the text that are to be carried out.
Figures
(1) Numbers in brackets within the descrip-
tions relate to the figures and the items
within those figures.

Leica M Series User Manual 5
Safety Instructions
Description
The individual modules fulfill the highest
requirements for observation and documenta-
tion of Leica stereo microscopes of the Mseries.
Intended use
ORefer to "Safety Concept" booklet
Non-intended use
ORefer to "Safety Concept" booklet
Never use M series microscopes or their compo-
nents for surgical procedures (e.g. on the eye)
unless they are specifically intended for that
purpose.
The instruments and accessories described in
this operating manual have been tested for
safety and potential hazards. The responsible
Leica affiliate must be consulted whenever
the instrument is altered, modified or used in
conjunction with non-Leica components that
are outside of the scope of this manual.
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims.
Place of use
ORefer to "Safety Concept" booklet
OElectrical components must be placed at
least 10 cm away from the wall and from
flammable substances.
OAvoid large temperature fluctuations,
direct sunlight and vibrations. These condi-
tions can distort measurements and micro-
graphic images.
OIn warm and warm-damp climatic zones,
the individual components require special
care in order to prevent the build-up of
fungus.
Responsibilities of person in charge of
instrument
ORefer to "Safety Concept" booklet
Ensure that:
OThe M series stereo microscopes and
accessories are operated, maintained and
repaired by authorized and trained person-
nel only.
OAll operators have read, understood and
observe this User Manual, and particularly
the safety instructions.

Leica M Series User Manual 6
Safety Instructions (Continued)
Repairs, service work
ORefer to "Safety Concept" booklet
OOnly original Leica Microsystems spare
parts may be used.
OBefore opening the instruments, switch off
the power and unplug the power cable.
Touching the live circuit can cause
injury.
Transport
OUse the original packaging for shipping or
transporting the individual modules of the
Leica M stereo microscopy series and the
accessory components.
OIn order to prevent damage from vibrations,
disassemble all moving parts that (accord-
ing to the user manual) can be assembled
and disassembled by the customer and
pack them separately.
Integration in third-party products
ORefer to "Safety Concept" booklet
Disposal
ORefer to "Safety Concept" booklet
Legal requirements
ORefer to "Safety Concept" booklet
EC Declaration of Conformity
ORefer to "Safety Concept" booklet
Health risks
Workplaces with stereo microscopes
facilitate and improve the viewing task,
but they also impose high demands on the eyes
and holding muscles of the user. Depending on
the duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
this reason, appropriate measures for reduction
of the workload must be taken:
OOptimal arrangement of workplace, work
assignments and work flow (changing tasks
frequently).
OThorough training of the personnel, giving
consideration to ergonomic and organiza-
tional aspects.
OThe ergonomic design and construction of
the Leica M stereo microscopy series are
intended to reduce the exertion of the user
to a minimum.

Leica M Series User Manual 7
Safety Instructions (Continued)
Direct contact with eyepieces is a
potential transmission method for
bacterial and viral infections of the eye.
The risk can be kept to a minimum by using
personal eyepieces for each individual or
detachable eyecups.
Light sources: Safety regulations
Protective measures of the manufacturer:
OUV protection screen in front of the speci-
men plane prevents the user from looking
directly into the UV rays.
ODummy filter carriers in the free positions
of the rapid filter changer prevent direct UV
radiation from reaching the eyes.
OUV filters are installed in the observation
beam paths to protect the eyes.
OThe stray-light protection on the lamp
housing prevents irradiation of the hands.
Warning
UV radiation could damage the eyes.
Therefore:
ONever look into the light spot on the speci-
men plane without a UV protection screen.
ONever look into the eyepieces if no excita-
tion filter is in the beam path.
ODo not select a white, strongly reflective
background for the specimen.

Leica M Series User Manual 8
Safety Instructions (Continued)
Supply unit
Always unplug the supply unit from the power
supply:
OWhen installing and disassembling the
lamp housing
OBefore opening the lamp housing
OWhen replacing the high-pressure mercury
lamp and other parts, such as the heat-
absorbing filter or the collector
ODuring maintenance work on the supply
unit
Lamp housing
Never open the lamp housing while the
lamp is switched on. Risk of explosion,
UV exposure, blinding!
Before opening the lamp housing, allow
it to cool off for at least 15 minutes.
Danger of explosion!
Never cover the air duct on the lamp
housing. Danger of fire!
Mercury lamp
OFollow the user manual and safety instruc-
tions provided by the lamp manufacturer,
and, in particular, the section on how to
proceed if a lamp breaks and releases
mercury.
OFor transport, remove the mercury lamp,
transport it in its original packaging and
protect moving parts in the lamp housing
using the transport anchors.
OWhen it has reached the end of its rated life
(follow the manufacturer's specifications
and the minute meter on the supply unit).
OTo minimize the risk of explosion, replace a
discolored mercury lamp promptly.
OLeica Microsystems assumes no liability
for damage caused by exploding, incor-
rectly installed or improperly used mercury
lamps.

Leica M Series User Manual 9
Contents
Transmitted-light Base Leica TL BFDF: Before First Use 30
Transmitted-light Base Leica TL BFDF 31
Leica TL RC / Leica TL RCI 32
Leica IsoPro Manual Cross-stage: Assembly 33
Leica IsoPro Motorized Cross-stage: Assembly 36
Cables: Terminals 40
Cables: Cable Duct 41
Cables: Diagram 42
Leica LED5000 MCI 43
Leica LED5000 MCI: Alternative Assembly 44
Leica LED5000 RL: Assembly 45
Leica LED5000 RL: Installing Optional Accessories 47
Leica LED5000 RL: Use 48
Leica LED5000 NVI: Assembly on High-Performance Stereo
Microscopes (Leica M125 C / Leica M165 C / Leica M205 C) 50
Leica LED5000 NVI: Installing Optional Accessories 53
Quick Start Guide
The Fastest Route to Success 56
Overview of an M Series Microscope 57
The Correct Interpupillary Distance 58
Using the Eyepieces 59
Focusing 60
Adjusting the Resistance of the Focus Drive 61
General Instructions 2
Safety Concept 3
Symbols Used 4
Safety Instructions 5
The Leica M Series
Congratulations! 13
A Step Towards Infinity 14
The Electronics: Comfort, Convenience and Safety for Your Experiments15
The Modular Design: Everything is Relative 16
Maximum Compatibility 17
On We Go 18
Assembly
Base and Focusing Column 20
Optics Carrier 21
Tube 22
Eyepieces 23
Objective 24
Objective Nosepiece – Assembly 25
Objective Nosepiece – Adjusting Parfocality 26
AX Carrier – Preparation 27
AX Carrier – Assembly 28
Transmitted-light Base Leica TL ST 29

Leica M Series User Manual 10
Changing the Magnification (Zoom) 62
Ratchet Steps and Magnification Levels 63
Parfocality: More Comfort and Convenience for Your Work 64
Iris Diaphragm 65
Eyepieces
Magnification Factors of the Eyepieces 67
Health Notes 68
Dioptric Correction 69
Dioptric Correction and Parfocality 70
Graticules 71
Photography & Video
Photography & Video 73
Photo Tubes and C-mounts 74
Trinocular Video/Phototube 50 % 75
Trinocular Video/Phototube 100 % 76
Microscope Carrier
The Microscope Carrier AX 78
The Objective Nosepiece 80
Objectives and Optical Accessories
The Different Types of Objectives 82
Bases
Transmitted-light Base Leica TL ST: Controls 84
Transmitted-light Base Leica TL ST: Operation 85
Transmitted-light Base Leica TL ST: Lamp Replacement 86
Transmitted-light Base Leica TL BFDF: Controls 87
Transmitted-light Base Leica TL BFDF: Operation 88
Leica TL RC / Leica TL RCI: Controls 89
Leica TL RCI: The Deflection Mirror 90
Leica TL RCI: Color Intensity and Temperature 91
Leica TL RC / Leica TL RCI: Operation 92
Leica TL RCI: Methods in Transmitted Light 93
Leica TL RCI: Relief Images 94
Using Filters 96
Leica IsoPro (Non-motorized): Controls 97
Leica IsoPro (Motorized): Controls 98
Accessories
Leica Hand Wheel and Foot Switch 100
Leica SmartTouch 101
Leica PSC Controller 102
Contents (Continued)

Leica M Series User Manual 11
Dimensional Drawings
Leica M125 C / Leica M165 C 104
Leica M205 C 108
Leica LED5000 MCI 111
Leica LED5000 RL 112
Leica LED5000 NVI 113
Specifications for the Bases
Transmitted-light Base Leica TL ST 115
Transmitted-light Base Leica TL BFDF 116
Leica TL RC / Leica TL RCI 117
Leica IsoPro Motorized XY Stage 118
Appendix
Calculating the Total Magnification and Field of View Diameter 120
Care, Maintenance, Contact Persons 121
Contents (Continued)

Leica M Series User Manual The Leica M Series 12
The Leica M Series

Leica M Series User Manual The Leica M Series 13
Congratulations!
Congratulations on obtaining your new Leica M series stereo microscope.
We are convinced it will exceed your expectations, as never before have
we applied our decades of experience in the areas of optics, mechanical
engineering and ergonomics in such an uncompromising manner.
The M series embodies all the qualities you associate with the name Leica
Microsystems: excellent objectives, high-quality engineering, and reli-
ability. Furthermore, the modular design ensures that the M series adapts
perfectly to your needs – no matter which accessories you require for your
tasks.
Though the reliability and robustness of Leica stereo microscopes is
legendary, like any high-tech product, the M series requires a certain
degree of care and attention. Therefore, we recommend that you read
this manual. It contains all the information you need regarding operation,
safety and maintenance. Simply observing a few guidelines will ensure
that even after years of intensive use, your stereo microscope will continue
to work as smoothly and reliably as on the very first day.
We wish you the best of success in your work – after all, you are now
equipped with the best tool!

Leica M Series User Manual The Leica M Series 14
A Step Towards Infinity
Ever since their introduction by Horatio S.
Greenough, stereo microscopes have worked
according to the optical principles based
primarily on Ernst Abbe's research. For over a
century, ingenious optics designers and engi-
neers have worked to push magnification, reso-
lution and image fidelity to the limit permitted
by optics.
In doing so, they have always been constrained
by the interrelation between three factors: the
higher a microscope's resolution, the lower the
available working distance. If one increases the
distance of the optical axes, the three-dimen-
sional image seen by the observer becomes
distorted – a sphere becomes an ellipse, a flat
surface curves toward the observer.
Limits are made to be broken.
The Leica M205 C is the world's first stereo
microscope with a zoom range of 20.5:1. This
accomplishment, however, was not enough for
Leica's engineers. With the new FusionOptic in
the M205 C, they have succeeded in going yet
another step beyond previous limits. In addition
to the increase in magnification, the resolution,
too, has been increased to up to 1,050 lp /mm*,
which corresponds to a resolved structure size
of 472 nm.
Of course, this performance increase benefits
your everyday work. Set up your specimens
on the microscope with comfortable freedom
of movement and discover details in stereo
microscopy that you could never see before.
* Leica M205 C with 1.0× planapochromat and 10× eyepieces

Leica M Series User Manual The Leica M Series 15
The Electronics: Comfort, Convenience and Safety for Your Experiments
Never before have electronics been used as
extensively in a Leica series as in the new
Mseries.Opticscarrier, tripod,base and illumina-
tor are all connected using electrical contacts –
which provides a number of advantages.
Reliability for your experiments
Exact reproducibility of results is an increas-
ingly common demand, particularly in research
and development. The continuous encoding
captures parameters such as the magnifica-
tion, the illumination, the position of the iris
diaphragm and more to the Leica LAS software.
Thus you always know the conditions under
which an image was acquired.
Fewer cables
A large part of the cables have been routed in
the interior of the column. Data are also trans-
ferred via the interface between the column,
the optics carrier and the carrier. The direct
result is that you need fewer cables – this not
only makes your workstation neater and more
comfortable, it even makes it appear larger.
Contacts not only transmit data, but also supply
the power.
Leica Application Suite (LAS) evaluates the
transmitted data and can restore the test
situation later.

Leica M Series User Manual The Leica M Series 16
Have a special request? Let us know!
Leica Microsystems enjoys an exceptional repu-
tation when it comes to devising customer-
specific solutions. If you have a special request
that cannot be met with standard parts, contact
your Leica consultant. We have a solution for
every problem.
The Modular Design: Everything is Relative
The Leica M series provides maximum flexibil-
ity in choosing equipment, thanks primarily to
the modular configuration and the compatibil-
ity that Leica has painstakingly maintained for
decades. The optics carriers, eyepieces, bases
and more can be combined in any way you
choose, allowing you to create the microscope
that best suits your needs.
Despite this, you will notice that the controls
and individual components do not differ signif-
icantly. Whichever configuration you choose,
you will quickly feel right at home.

Leica M Series User Manual The Leica M Series 17
Leica engineers were careful to ensure that the
new M series – like its predecessors – remains
compatible with existing series. This means
that objectives, bases, tubes and so on can be
reused.
Objectives
All new objectives of the M series are parfocal,
meaning that when used with the objective
nosepiece, they can be replaced while keeping
the specimen in perfect focus.
If you prefer, you can continue to use the previ-
ous objective series. In this case, parfocality is
no longer guaranteed.
Tubes
The interface between the optics carrier and
the tube has remained the same, so exist-
ing tubes fit the new M series. The new tubes
are designed for eyepieces with field number
23,
while the predecessor models were only
designed for field number 21, resulting in a
smaller object field.
Eyepieces
However, the new M-series eyepieces models
have an audible and tangible click to provide
immediate feedback in case of accidental
adjustment.
Maximum Compatibility

Leica M Series User Manual The Leica M Series 18
On We Go
If your new Leica microscope has already been
assembled and commissioned by your Leica
consultant, click here to skip through the instal-
lation instructions and go directly to the Quick
Start Guide on page 55.
If, on the other hand, you are assembling the
microscope yourself, continue with the "Assem-
bly" chapter, which begins on page 19.

Leica M Series User Manual Assembly 19
Assembly

Leica M Series User Manual Assembly 20
The first step is to connect the focusing column
of the M series to the corresponding base.
Tools used
OHex socket screwdriver, 3 mm
Assembling the column adapter
1. Securely install the column adapter on the
column using the four included screws.
Assembling the focusing drive
2. Securely screw the focusing column to the
base using the six included screws.
Base and Focusing Column