N-BIOTEK NB504CIR User manual

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NB504CIR Operation Manual
Model : NB504CIR

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TABLE OF CONTENTS
NO.
CONTENTS
PAGE
1
SAFETY AND GENERAL PRECAUTION
3
2
TRANSPORTATION, STORAGE, AND LOCATION OF INSTALLATION
5
3
PREREQUISIT AND CONFIGURATION
7
4
FEATURES AND SPECIFICATIONS
10
5
CONTROL PANEL
11
6
OPERATION
12
7
SAFETY SWITCH & MAINTENANCE
14
8
TROUBLESHOOTING
15

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1. SAFETY AND GENERAL PRECAUTION
1. 1 General Information on Precaution
This equipment must be operated as described in this manual. if operational guideline is not
followed, equipment damage and personal injury can occur.
Please follow this mark to prevent equipment damage of personal injury.
If you don't keep this caution, you can get injured as well as a property loss.
1.1.1 Safety warning symbols
1.2 Precaution for using the power cable
Do not make the power plug be pressed by back of the product.
(A space between the product and the plug must be 30cm at least.)
The power outlet must be only for this product.
(Using various products simultaneously can cause a fire.)
Clean the power plug with a dry towel and connect it properly.
(Foreign substances or unsafe connection can cause a fire.)
Do not bend the power cable hardly and do not make it to be pressed by
heavy products. (When it is damaged, it can cause a fire.)
Do not touch the power cord with wet hands.
(It can cause an electric shock.)
Do not use the damaged power cord and outlet.
(It can cause an electric shock and a fire.)
When you see smoke coming from the product or smell burning, you have to pull out
the power cord and stop using it.
(It can cause an electric shock and a fire.)

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1.3 Precaution for ground connection
Please ground before use the product, if you don't ground, you could get an electric shock
when malfunction or an electric leakage occurs.
At the place where you can't ground,
* Please buy the equipment to prevent any electrical leakage.
* An electric shock, an electric leakage and a fire can be occurred without an electric
leakage breaker.
Do not ground to these places; Gas Pipe, water pipe, pipe, lighting rod,
telephone wire, etc.
* Wrong ground connection can cause electrical leakage which eventually results in fire.
If you don't have the outlet for AC 220V, then bury it under the ground after connecting
the ground line to copper plate.
* No ground connection can bring an electrocution, an electric leakage and a fire.
1.4 Precaution for use
You must not disassemble, fix and remodel the product by yourself.
(You can damage the product throughout a fire and malfunction or get a property loss as
well as experimental loss.)
Do not use the product for different purpose.
(It can cause malfunction or poor function. Consequently, you will get a wrong result.)
Do not use an inflammable spray near the product.
(The switch and other electric connection parts can cause a fire.)
When you use inflammable substances such as benzene, thinner, alcohol and LP gas,
please be careful. (It can cause a fire and an explosion.)
To prevent water and experiment material from going into the control panel during
the experiment, make sure to clean the control panel with a dry cloth.
(It can cause an electric leakage and a fire.)
Do not wash the product with excessive quantity of water, thinner, benzene and Petroleum.
(It can cause an electric leakage, and malfunction or damage on the surface.)

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When you don't use the product or clean it, please pull out the power plug.
(It is to prevent an eclectic leakage.)
Open and close the door softly and please use a door knob.
(A heavy shock can damage the product and breakdown the operating part.
Also, your hands can be stuck between the door and body.)
Do not detach the built-in lamp and electrical devices.
(It can cause an electric shock and a fire.)
Please be sure to prevent foreign substances from getting into the sealing silicon of the door.
(The inflow of open air can cause the change of temperature in chamber and discoloration of
the packing part by a foreign substance.)
2. TRANSPORTATION, STORAGE, AND LOCATION OF INSTALLATION
2.1 Transportation
DO NOT try to slide or tilt the unit
Lift the unit on each corner of bottom with the aid of 2 people.
Permissible ambient temperature range for transport: -10℃to 60℃.
2.2 Storage
Do not keep it at place in High Humidity. Permissible ambient humidity: max. 70%
storage in a cold location is the place you transfer the unit to the installation site for start-up,
condensation may form. In this case, wait at least one hour until the CO2 incubator has
attained temperature and is completely dry.
Please check the voltage & Hertz written on serial label.
(Over-voltage, under-voltage can damage the product and poor performance.)
Do not install at a humid place.
(It causes an electric leakage accident and a corrosive of the product.)
Keep this product out of the direct ray of sun and do not install at a hot place or
a place that is near an electric heat.
(The proper indoor temperature is 20℃~ 30℃.)

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2.3 Location of installation and ambient conditions
Do not put flammable substances near the product. (It may cause a fire.)
When you install the product, you are required to keep the distance at least 30cm
from the wall. To completely separate the unit from the power supply, power plug
must be disconnected. Install the unit in the way that the power plug is easily accessible
and can be easily pulled in case of danger.
Install the unit at a flat surface, free from vibration and in a well-ventilated location.
(If the ground is not flat, it may cause an excessive vibration of the product.)
When you move the product, do not lay down to its side or reverse the head
to bottom. (It may cause a malfunction.)
When you move the product, hold the door and other movable parts of
the product with a tape. (When the product is moved, the movable door can cause
injury of you and damage of the product.)
When you move the product, you must hold up the product.
(Pushing or pulling the product can damage the bottom part of the product.)

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3. PREREQUISIT AND CONFIGURATION
3.1 Prerequisit
Inspection of Boxes
When you have received the instrument which is packed on pallet, inspect the box carefully for any
damages that may have caused any damages to product during shipping.
Please report any damage to the carrier or to your local N-BIOTEK distributor immediately.
Location
The incubator is designed to operate at temperature 4℃. Maximum Room Temperature is 32℃.
To avoid place for use this incubator is as below.
1. Near heater or freezer (if it may generate heat and affect temperature control of incubator)
2. Near equipment generating heat or cold air to incubator
3. Directly sunlight exposed to incubator
4. Uneven ground or table head
5. The place where is being vibrated
Caution
Make sure that the rotor fixing nut is securely fastened before running.
If the unit runs with rotor loosen, it may cause the serious motor shaft damage in which could lead the
breakage inside of the unit.
Make sure that the cold trap bottle is well fitted with cold trap.
Make sure that the cold trap where produces -20℃should be maintained in clean condition for better effect.
Make sure that the passive vacuum release is at right position.
Make sure that the IR lid is well closed.

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3.2 Configuration
<Figure #1>
1. Safety Lid
6. Chamber, Rotor
2. Vacuum Gauge
7. Front Cover
3. Control Panel
8. Main Power
4. Cold Trap
9. Safety Switch
5. IR Lid
<Figure #2>
Note:
There is a vertical empty gap on rotor.
That gap must face to chamber, not to
rotor inside in order to create centrifugal force.

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<Figure #3>
Passive vacuum release valve at CLOSED position at OPEN position
(When you need to force the vacuum out)
(e.g. In case of power failure(black out) during use)
<Figure #4>
At the rear of NB504CIR, there is a port where air in chamber comes out as vacuum fills up in chamber.
There might be a sound of air coming through this port until chamber gets to vacuum condition.
<Figure #5>
Temperature sensor for chamber on LID

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4. FEATURES AND SPECIFICATIONS
4.1 Features
1. IR concentrator uses centrifugal force with heat, IR and vacuum to evaporate solvents faster
2. Transparent IR-emitting glass lid
→With the use of infrared ray, the sample can be concentrated efficiently, quickly under
vacuum condition.
3. Cold trap (-20℃)
→Cold trap where produces -20℃helps evaporate more faster and efficiently plus
minimize contamination and sample loss.
4. Brushless DC motor
→Induction motor barely makes noise and vibration. Plus, this gives less maintenance required.
5. User-friendly operation designed
6. Compact & mobile system
→It is suited for individual researchers with limited footprint.
4.2 Specification
Items
NB504CIR
Temperature
Range
+4℃to 80℃
Increment
0.1℃
Controller
Microprocessor Digital PID
Heating Mode
3 Selectable mode (IR, IR/HEAT, HEAT)
Timer
99hrs 59min
Capacity
1.5ml micro-tube x 132ea, 15ml tube x 12ea, 50ml tube x 8ea,
250ml x 4eq, 96 well microplate x 2ea
RPM
Max. up to 1,500RPM
(no display of RPM on control panel)
Vacuum
Pump
Chemical resistance PTFE coated Diaphragm pump
Ultimate pressure
2 mbar
Gauge
Analogue vacuum gauge
Cold Trap
-20℃, 2 liter trap
Weight
138 kg
Overall dimension
600(W) x 630(D) x 1,085(H)mm
Power
110/220V, 50/60Hz

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5. CONTROL PANEL
When overheating is detected by the safety device, GREEN light will be on
Shows which mode of Heating is on by displaying different colors
- IR: GREEN light
- IR/HEAT: ORANGE light
- HEAT: RED light
Use to select IR, IR/HEAT, or HEAT mode
- IR: IR lid heating only
- IR/HEAT: IR lid and chamber heating simultaneously
- HEAT: Chamber heating only
Use to select OFF, STANDBY(preheat), or RUN mode
- OFF: Power off the centrifuge, vacuum pump, and heater (display will be turned off)
- STANDBY: Heating up without centrifuge operation prior to concentration (Preheat)
- RUN: Centrifuge operation and vacuum start
[Note] On STANDBY mode, the set time continues to flow regardless of whether the centrifuge is operating
or not.
Shows the set value and actual measured values of temperature and time
Use to display TIME remaining / press again to get back to Temperature
Use to set TEMPERATURE and TIME
Use to adjust the set temperature and time in setting mode
Use to START or STOP the operation (and reset the timer - reset to the previous set value)
Pilot Lamp of Overheating
Pilot Lamp of Heating
HEAT Mode Selector
OPERATION Mode Selector
Temperature and Time Control Display
TIME Button
SET Button
INCREASE / DECREASE Button
START / STOP Button
2
3
1
1
2
4
4
3
5
5
6
8
9
7
6
7
8
9

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6. OPERATION
The location where you are desired to use it should be flat, balanced surface to keep the unit stable.
Before connecting the power cable to the plug, make sure that the unit is turned off to avoid any
electric shock.
[Start Operating]
1) Power On
- Please check figure #1(pg. 8) where you can see main power switch.
2) Check Cold Trap
- Make sure that cold trap is well fitted in its place. If not, proper vacuum won’t be generated.
- If the cold trap is necessary, turn Cold trap Power S/W on.
(* After turn the operation mode to “STANDBY”)
There is a switch for cold trap(TRAP) right next to control panel.
3) Rotor Installation
- Make sure that rotor and tube or bottle should be balanced for installation.
- Unbalance installation on those parts will cause a severe malfunction.
[Temperature Setting]
1) Turn the operation mode to “STANDBY”. Then, display shows the current temperature of chamber.
2) Choose the desired heating mode (IR / IR&HEAT / HEAT).
3) Press SET button once and LED display will flicker with showing the current set value of
temperature.
In this status, you can adjust the set temperature by using INCREASE & DECREASE button.
4) After setting, press SET button again to SAVE.
At the same time as saved, it will move on to next step, Time Setting.
[Note] If the set value is higher than current temperature of chamber, then heating starts with HEAT
pilot lamp on. When the temperature is almost reached to set temperature, the pilot lamp will be
flickering.
[Note] Temperature setting range is from 4°C to 80°C. If operating temperature is needed to be below
ambient, please turn on CHAMBER to activate compressor. Otherwise, compressor remains off
for longer life span.

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[Time Setting]
1) At the same time as set temperature saved, it will move on to Time Setting.
On LED display, ‘HOUR’ section will flicker.
2) Adjust to set ‘HOUR’ section by using INCREASE & DECREASE button.
3) Press SET button again and it will move on to ‘MINUTE’ section.
Adjust to set ‘MINUTE’ section by using INCREASE & DECREASE button.
4) After setting all, press SET button again to SAVE.
“SAVE” will be shown on the display and then move on to current temperature.
[Note] For non-stop operation, the time setting on the timer should be at “00:00”.
[Note] When the set time is expired, the device will automatically stop the operation and “END” will be
shown on the display. To restart the operation, press START/STOP button.
(The set value will be reset to the previous set value.)
[Operating]
After Temperature & Time setting is completed, turn the operation mode to “RUN”.
When rotor starts to rotating, concentration is begun.
[Note] Vacuum gauge at 85~90 is regarded as normal operation.
[Note] Make sure to keep away from the LID during operation if IR heat mode is set.
[General Information]
- In IR or IR&HEAT mode, temperature set-up range is limited up to 65°C, because IR lid has limited
heating capacity.
- In IR mode, temperature of IR lid is higher than set temperature because only IR lid is heating and
warming up the air inside the chamber.
- There are 4 ways to stop the centrifuge during the operation.
1) Turn the operation mode to “STANDBY” : It will stop the centrifuge and vacuum pump.
The display will keep show the current temperature.
[Note] On STANDBY mode, the set time continues to flow regardless of whether the centrifuge
is operating or not.
2) Turn the operation mode to “OFF” : It will stop the centrifuge, vacuum pump, and heater.
The display will be turned off.
3) Press START/STOP Button : It will stop the centrifuge, vacuum pump, and heater.
The display will show “STOP”.
4) Turn off the main power : It will immediately stop all actions.

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[Note] When restart the operation or turn on the power after 2), 3), or 4) : The set value will be reset
to the previous set value.
- Cold trap power can be turned on only when the main power is on.
- Cold trap power is turned on/off only through the Cold Trap Power S/W.
Cold trap power cannot be turned off from the control panel.
[Note] Turning off the main power S/W also turns off the cold trap power. In this status, turning on
the main power S/W leads to cold trap power on automatically.
To turn off the cold trap power properly, it is recommended to use the cold trap power S/W.
Also, it is recommended to turn off the cold trap power first and then turn off the main power
when turning off the main power.
- When you turned off and on the cold trap power, it is recommended to wait about 20 minutes for the
compressor working properly.
7. SAFETY SWITCH & MAINTENANCE
[Safety Switch]
Safety S/W is a device to shut off the overheating in the chamber and
temperature rising by malfunction.
Place safety dial at least 10°C higher than the set temperature as its
accuracy is more than 8°C.
If the temperature of concentrator is not able to increase, check if this
safety dial blocks the temperature increasing.
This is the safety device for preventing temperature from rising caused by any circumstances.
You can identify its activation by checking lighting of pilot lamp.
[Maintenance]
In order to keep in good condition for longer use of unit, it is highly recommended to clean and check the all
components after finishing each concentration. when you clean inside chamber or some part, you should
disconnect the power first.
①Clean up all spilled solvent in chamber
→Remove liquids in chamber and decontaminate all remains on the surface of chamber.
②Clean up the trap and cold trap
→Make sure that the trap is cleaned after end of each experiment.
③Deforest inside cold trap
→Make sure that the cold trap is cleaned after end of each experiment.

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④Remove the rotor nut and clean the rotation shaft for the next use.
⑤Clean the exterior surface of unit with the dried cloth
⑥After use of chamber compressor, you are required to run the chamber at 50°C or higher in order to
remove cold inside. This is an important procedure for longer life-span and for normal concentration
speed next time.
8. TROUBLESHOOTING
PROBLEM
CAUSE
CORRECTIVE ACTION
Unit will not operate
Unit not connected to
electrical power
Connect unit to proper
electrical receptacle
Circuit breaker blown
Correct electrical problem and
reset circuit breaker
Lid open
Close lid
Excessive vibration
Sample vials not located
symmetrically in rotor
Reposition sample vials
Sample odor in lab
Vent hose exhausting into
lab area
Redirect hose to fume hood
Evaporation rate is
reduced
Heater inoperable
Contact N-BIOTEK
Vacuum pump failure
Check pump
Liquid un vacuum line
Clear vacuum line
Evaporation rate is
reduced
(continued)
Obstruction in hose
Remove obstruction or replace
hose
Lack of adequate vacuum
See below
Temperature set point too low
Increase temperature set point
No vacuum/poor vacuum
Liquid in glass trap
Empty trap
Leaks in lines or
connectors or gasket
Locate and repair
Foreign material on lid gasket
Clean gasket and lid
Manufactured by : N-BIOTEK, Inc.
402-803 Techno park, 655 Pyeongcheon-ro,
Yakdae-dong, Wonmi-gu, Bucheon-si,
Gyeongi Province, Korea
Tel : [phone] | E-mail : [e-mail]