Agilent Technologies 1290 Infinity II 2D-LC Solution User manual

Agilent 1290 Infinity II 2D-LC Solution
User Guide
2D-LC - User Guide

2D-LC - User Guide
Notices
Document Information
Document No: SD-29000383 Rev. B
Part No: G2198-90001 Rev. B
EDITION 02/2020
Copyright
© Agilent Technologies, Inc. 2012-2020
No part of this manual may be repro-
duced in any form or by any means
(including electronic storage and retrieval
or translation into a foreign language)
without prior agreement and written con-
sent from Agilent Technologies, Inc. as
governed by United States and interna-
tional copyright laws.
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Warranty
The material contained in this document
is provided “as is,” and is subject to being
changed, without notice, in future edi-
tions. Further, to the maximum extent
permitted by applicable law, Agilent dis-
claims all warranties, either express or
implied, with regard to this manual and
any information contained herein, includ-
ing but not limited to the implied warran-
ties of merchantability and fitness for a
particular purpose. Agilent shall not be lia-
ble for errors or for incidental or conse-
quential damages in connection with the
furnishing, use, or performance of this
document or of any information con-
tained herein. Should Agilent and the user
have a separate written agreement with
warranty terms covering the material in
this document that conflict with these
terms, the warranty terms in the separate
agreement shall control.
Technology Licenses
The hardware and/or software described
in this document are furnished under a
license and may be used or copied only in
accordance with the terms of such
license.
Restricted Rights Legend
U.S. Government Restricted Rights. Soft-
ware and technical data rights granted to
the federal government include only
those rights customarily provided to end
user customers. Agilent provides this
customary commercial license in Soft-
ware and technical data pursuant to FAR
12.211 (Technical Data) and 12.212
(Computer Software) and, for the Depart-
ment of Defense, DFARS 252.227-7015
(Technical Data - Commercial Items) and
DFARS 227.7202-3 (Rights in Commer-
cial Computer Software or Computer
Software Documentation).
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could result
in damage to the product or loss
of important data. Do not proceed
beyond a
CAUTION notice until
the indicated conditions are fully
understood and met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in personal injury or death.
Do not proceed beyond a WARN-
ING notice until the indicated
conditions are fully understood
and met.

2D-LC - User Guide 3
In This Book
This manual covers the Agilent 1290 Infinity II 2D-LC Solution and 2D-LC
Acquisition software (G2198AA).
1Introduction
This chapter describes the product of Agilent 1290 Infinity II 2D-LC Solution.
2Conceptsof2D-LC
This chapter describes the concepts of Agilent 1290 Infinity II 2D-LC Solution.
3 Compatibility Matrix
The compatibility matrix provides information about installation and execution
prerequisites with respect to hardware, firmware and the operating system. Agilent
2D-LC Software is an OpenLAB CDS ChemStation Edition plug-in.
4Installation
This chapter describes the installation of the Agilent 1290 Infinity II 2D-LC Solution.
These installation instructions are valid for the modes standard heart-cutting,
multiple heart-cutting, high resolution sampling and comprehensive 2D-LC except
the connections to the 2D-LC valve. These connections depend on the method, see
chapter "Run the System for detailed information.
5 Method Parameters
This chapter provides background information on method parameters. It helps to
optimize methods in Agilent 1290 Infinity II 2D-LC Solution in the modes standard
heart-cutting, multiple heart-cutting, high resolution sampling and comprehensive
2D-LC.
6 Run the System
This chapter describes how to run the Agilent 1290 Infinity II 2D-LC Solution in the
modes standard heart-cutting, multiple heart-cutting, high resolution sampling and
comprehensive 2D-LC.

2D-LC - User Guide 4
7 Investigate the effects of using different gradients in the 2Dimension
This chapter describes, how shifted gradients in the second dimension can be
used to enlarge the accessible two-dimensional separation space.
8 Data Analysis
This chapter descibes the analyzation of data in 2D-LC and is separated in a
section heart-cutting 2D-LC and a section comprehensive 2D-LC.
9 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic features
and the different user interfaces.
10 Error Information
This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions how to recover from error conditions.
11 Maintenance
This chapter describes the maintenance of the 2D-LC Solution.
12 Parts for Maintenance
This chapter provides information on parts material required for the solution.
13 Alternative ways to install the System
This chapter describes alternative ways to install and setup the system.
14 Agilent 2D-LC Solution with the selectivity of mass selective detection
(MSD)
This chapter describes the different options to use the Agilent 2D-LC Solution with
mass selective detection (MSD).

2D-LC - User Guide 5
15 Theoretical Background
This chapter gives the theoratical background of 2D-LC and describes the system
components (soft- and hardware) of the Agilent 1290 Infinity II 2D-LC Solution.
16 Appendix
This chapter provides addition information on safety, legal and web.

2D-LC - User Guide 6
Contents
1Introduction10
Product Description 11
Features 13
2Conceptsof2D-LC14
Concepts of 2D-LC 15
Heart-Cutting 2D-LC (LC-LC) 16
Multiple Heart-Cutting and High Resolution Sampling 2D-LC 17
Comprehensive 2D-LC (LCxLC) 29
Triggering of 2D-LC 31
Active solvent Modulation (ASM) 35
3 Compatibility Matrix 45
Supported Chromatographic Data Systems 46
Supported Drivers 47
Supported Operating Systems 48
Supported Firmware 49
Available Languages 50
PC Requirements 51
4Installation52
Delivery checklist 53
Options 54
Recommendations for instrument setup 58
Installation of the 2D-Valves 75
2D-LC Software Configuration 84
Installation of Active Solvent Modulation (ASM) 86

2D-LC - User Guide 7
5 Method Parameters 87
Method Parameters Standard Heart-Cutting 2D-LC 88
Method Parameters Multiple Heart-Cutting and High Resolution Sampling
2D-LC 104
Method Parameters Comprehensive 2D-LC 127
Method Development of Active Solvent Modulation (ASM) 141
6 Run the System 146
Connect the capillaries to the 2D-LC valve 147
Standard Heart-Cutting 2D-LC 148
Multiple Heart-Cutting and High Resolution Sampling 2D-LC 159
Comprehensive 2D-LC 175
Active Solvent Modulation (ASM) 189
7 Investigate the effects of using different gradients in the
2Dimension 192
Investigate the effects of using different gradients in the 2Dimension 193
8 Data Analysis 200
Data Analysis for Heartcutting 2D-LC (LC-LC) 201
Data Analysis for Comprehensive 2D-LC (LCxLC) 219
9Troubleshootingand Diagnostics 231
Overview of the Module’s Indicators and Test Functions 232
User Interfaces 233
Agilent Lab Advisor Software 234
The Basic Principle of Troubleshooting 235
Recommended Tests to Conclude Troubleshooting 245
10 Error Information 246
What Are Error Messages 247
General Error Messages 248
Module-Specific Error Messages 254

2D-LC - User Guide 8
11 Maintenance 258
Introduction to Maintenance 259
Warnings and Cautions 260
Overview of Maintenance 262
Cleaning the Module 263
Correcting Leaks 264
Replace Valve Heads 266
Replacing Parts of the Valve Head 272
Replacing the Fuses of the Infinity Valve Drive 274
Replace the Module Firmware 276
12 Parts for Maintenance 277
2D-LC Loops 278
2D-LC Capillaries 279
ASM Capillaries 280
Pressure Release Kit 281
2D-LC Easy Starter Kit 282
Valve Drive Parts 283
Valve Driver Parts Infinity II 284
Valve Head Parts 285
Vale Options Overview (for 2D-LC) 287
MS Diverter Valve 291
Valve Options Overview (for G7116B) 293
Additional Heater Devices 295
Accessories and Consumables (for G7116B) 297
InfinityLab Quick Connect and Quick Turn Fittings 299
Capillary Kits 303
Important Customer Web Links 304
13 Alternative ways to install the System 305
Legacy Stack Configuration 306

2D-LC - User Guide 9
14 Agilent 2D-LC Solution with the selectivity of mass selective detection
(MSD) 311
Agilent 2D-LC Solution with an Agilent Single Quadrupole Detector 312
Agilent 2D-LC Solution with high-end MS detection 318
15 Theoretical Background 322
Theoretical basis of 2D-LC 323
2nd dimension as detector 331
Successful Mode Combinations 333
Solvent Elution Modes 334
Practical Issues 340
16 Appendix 342
General Safety Information 343
Waste Electrical and Electronic Equipment (WEEE) Directive 350
Radio Interference 351
Sound Emission 352
Solvent Information 353
Further Information 354
Agilent Technologies on Internet 355

2D-LC - User Guide 10
1Introduction
Product Description 11
Features 13
This chapter describes the product of Agilent 1290 Infinity II 2D-LC Solution.