Nikon Ee1078 User manual

Nikon Wide Field Microscope Ee1078
Quick start
Erasmus Optical Imaging Centre document version 3
1
Start-up system
•Switch on the power socket (#1)
•Switch on Mercury lamp control unit switch on and ignite (#2)
oWait ~10 seconds for the device to load
oHold ignite button until orange LED is active
•Start the PC before other hardware is active
oUser account Nikon1078, no password required
•Switch on the power socket (#3)
•Switch devices on in the order of:
oHalogen lamp
oIncubator control-box
oMicroscope stand located at the right, behind the serial plug
oStage controller located at the back of the device
oShutter controller
•Start imaging software Metamorph
oSelect "User" account
•Upon error; drag error message from behind
Metamorph logo
oMessage: ASI MS2000 not detected on COM1
oChoose: Retry
Shut down system
•For live cell imaging:
oRemove Tokai Hit incubator from the stage and place it on the table
oClose the Tokai Hit incubator with a plastic well
•Clean objective carefully
oRemove oil with lens paper:
oHold lens paper tight between your fingers and wipe over objective
oWet your lens paper with 2-propanol and wipe 3-4 times more
•Cover stage with lens paper cleaning box
•If you are the last user of the day
•Shut down all devices
oShut down Mercury lamp at last
oCamera power is switched at the adapter box at the floor
•Switch off the power socket located at the floor

Nikon Wide Field Microscope Ee1078
Quick start
Erasmus Optical Imaging Centre document version 3
2
Controlling intensity mercury lamp
•Fluorescence can be detected with use of the Mercury lamp
oChoose illumination settings according to your sample
•Intensity of the light can be controlled with neutral density (ND) filters.
oLocated between the actual lamp (right) and the microscope stand.
oPress inwards to reduce intensity by 4 or 8 times (ND4, ND8)
Taskbar
•When the taskbar is not visible
oLoad the taskbar via: Journals>Taskbar>Load Taskbar
oLocation C:\MM\TASKBARS\
Restore
illumination
settings
Open acquire window
Open stream
window
Open MDA configuration
Move stage with cursor
Select
illumination
setting to
eyepiece
Select illumination
setting to camera and
start Live mode
•If needed: reset the illumination settings with: "Restore OIC settings"
•Open the Acquire window
•Configure Stream acquisition or start MDA configuration
•Move stage by clicking inside live image window (distance from centre equals
movement)
•Select illumination settings for Eyepiece (left) or camera (right)

Nikon Wide Field Microscope Ee1078
Quick start
Erasmus Optical Imaging Centre document version 3
3
Acquire window
•Open via Taskbar button “Acquire”
•Acquire an image
•Set exposure time
•Camera gain settings
•Show/Stop live view
Multi-Dimensional Acquisition (MDA)
Combine multiple tasks: z-stack, timelapse, streaming, multiple positions, multiple
wavelengths, use of journals.
Select acquisition mode(s) in Main
•Continue with the tabs shown
left:
oSaving: select filename and
folder
oSelect illumination settings
o… options for acquisition
modes
•When setup is done press acquire
•Save or load settings of
experiment setup, with Save/Load
State buttons
•Live mode can be started from MDA window