Nippon AQUA User manual

AQUA kit for MA-3 Solo
INSTRUCTION MANUAL
NIC-TD-0000187-01
NIPPON INSTRUMENTS CORPORATION

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation
CONTENTS
1. OUTLINE ..........................................................................................................................1
SAFETY PRECAUTIONS .........................................................................................1
1.1.1 MEANINGS AND EXPLANATIONS OF SYMBOLS ........................................ 1
1.1.2 CAUTIONS CONCERNING INSTALLATION ...................................................1
1.1.3 CAUTIONS CONCERNING HANDLING ..........................................................1
2. OUTLINE OF INSTRUMENT........................................................................................2
SIMPLIFIED EXPLANATION ..................................................................................2
SAMPLING FLOW ....................................................................................................2
CONSTITUTION .......................................................................................................3
3. INSTALLATION ..............................................................................................................5
4. OPERATION.....................................................................................................................6
NECESSITIES FOR MEASUREMENT....................................................................6
ARTICLE RECOMMENDED FOR MEASUREMENT............................................6
PREPARATIONS OF REAGENTS............................................................................6
BLANK PROCESS OF MERCURY COLLECTOR TUBE.......................................7
OPERATIONAL PROCEDURE.................................................................................7
CLEANING OF GLASSWARE ............................................................................... 10
5. MAINTENANCE............................................................................................................ 11
DAILY INSPECTION............................................................................................... 11
RECOMMENDED REPLACEMENT COMPONENTS.......................................... 11
ERRORS AND WARNINGS.................................................................................... 11
LEAK CHECK..........................................................................................................12
6. SPECIFICATION ...........................................................................................................13
7. CONTACT ADDRESS ...................................................................................................14

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation 1
1. OUTLINE
SAFETY PRECAUTIONS
The AQUA kit for MA-3 Solo is an option unit for the MA-3 Solo.
Before using this unit, read the instruction manual of the MA-3 Solo carefully.
To use this product safely, observe the following precautions.
Nippon Instruments Corporation will not take any responsibility for accidents caused
by a user’s carelessness.
In the case of mishandling, a user may be killed or suffer a serious
injury immediately.
In the case of mishandling, a user may be killed or suffer a serious
injury.
In the case of mishandling, a user may suffer an injury or material
damage is expected.
Install the instrument on a horizontal place with sufficient
strength.
Otherwise, an injury or an instrument failure caused by an
overturn or a fall may result.
Before using a reagent, procure and administer the safety data
sheet (SDS).
When using a reagent, wear protective equipment and carry out
ventilation sufficiently. Reagents to be used for the instrument
include acute toxic and corrosive substances.
Pay special attention when handling the glass parts. There is a
risk of injury.
Dispose of effluent appropriately after using the instrument.
When handling a mercury collector tube for a measurement or
the like, take care not to get burned.
When handling, put on protectors such as heat-resistant gloves,
and use boat tongs.
Danger
Warning
Caution
Caution
Warning
Caution

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation 2
2. OUTLINE OF INSTRUMENT
SIMPLIFIED EXPLANATION
This unit is the option for MA-3 Solo for the measurement of extremely low concentration
liquid samples like environmental water. The mercury contained in the liquid samples is
reduced and vaporized, and then collected to the mercury collector tube. The amount of
mercury is determined by MA-3 Solo.
SAMPLING FLOW
When sulfuric acid is added to sample and then stannous chloride solution is added, bivalent
mercury ions (Hg2+) are reduced to zerovalent metal mercury and changed to mercury gas
by bubbling. The collection concentration is carried out selectively for the mercury gas as
gold amalgam.
Hg2+＋SnCl2 → Hg0↑
*Acid mist and steam generated by bubbling are removed using a KOH solution and a mist
catcher because they obstruct a measurement.
* Although sample with small quantities of interfering substances, such as raw water for tap
water, can be measured only by adding sulfuric acid, it is impossible to obtain a precise and
accurate measurement result for sample containing interfering components.
Exhaust Port
Sample
Reagent (sulfuric acid,
stannous chloride solution)
KOH solution
Mist catcher
Mercury
Collector Tube

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation 3
CONSTITUTION
No.
Name
Qty
Description
1
Impinger outside cylinder
5
2
Impinger inside cylinder
2
3
Reagent container
5
4
Impinger stand
1
5
Cleaning bottle
1
6
Joint Clamp
2
7
Mist catcher
2
8
Syringe
10
9
L-Cysteine (1 g)
1
10
Tygon Tube
2m
φ6 Including spare
11
Mercury Collector Tube connection piping
1
Including one mist catcher
12
PTFE Tube
0.3m
φ4×6 Including spare
1
2
3
4
12
11
10
9
8
7
6
5

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation 4
No.
Name
Qty
Description
13
Mercury Collector Tube
5
65L
14
Collector Tube Holder
5
No.
Name
Qty
Description
15
Carrying Case
1
13
14
15

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation 5
3. INSTALLATION
Install the instrument on a horizontal place with sufficient
strength.
Otherwise, an injury or an instrument failure caused by an
overturn or a fall may result.
Connect the tubing as follows.
<Rear side>
<Front side>
*During the installation, do not close the inside cylinder of the 1st impinger.
Caution
PTFE Tube (approx. 5cm)
Tygon Tube (approx. 80cm)
Tygon Tube (approx. 80cm)
OUTLET – 1st Impinger IN
Tygon Tube (approx. 25cm)
1st Impinger OUT – 2nd Impinger IN
Mercury collector tube
connecting tube

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation 6
4. OPERATION
Before using a reagent, procure and administer the safety data
sheet (SDS).
When using a reagent, wear protective equipment and carry out
ventilation sufficiently. Reagents to be used for the instrument
include acute toxic and corrosive substances.
Pay special attention when handling the glass parts. There is a
risk of injury.
Dispose of effluent appropriately after using the instrument.
NECESSITIES FOR MEASUREMENT
(1) Distilled Water (used to clean impinger after measurement)
(2) Effluent Tank (used to keep effluent after measurement)
(3) Reagents
10% Stannous Chloride Solution
1:1 Sulfuric Acid
KOH (Potassium Hydroxide)
(4) Standard Mercury Liquid
ARTICLE RECOMMENDED FOR MEASUREMENT
(1) Apparatus for Dispensing Solution, Such As Micropipette
PREPARATIONS OF REAGENTS
*Use reagents that contain little mercury, such as ones for mercury analyses, for precise
analyses or for toxic metal measurements.
Sulfuric Acid (1:1)
Preparation
Procedure
Pour distilled water into a beaker and add the same volume of sulfuric
acid slowly while cooling and stirring the solution.
*To prevent an accident caused by heating at the time of dilution,
we recommend that the following reagent be used:
Recommended
Reagent
64% sulfuric acid 500 mL [198-11565] manufactured by Wako Pure
Chemical Industries
Warning
Caution

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation 7
10% Stannous Chloride Solution
Preparation
Procedure
*To prevent deterioration caused by a lapse of time, we recommend
that the following reagent be used:
Recommended
Reagent
Stannous chloride for mercury analysis [36522-08] manufactured by
Kanto Chemical
1 mol/L KOH Solution (Potassium Hydroxide Solution)
Preparation
Procedure
Melt 11.2 g of potassium hydroxide using pure water to make the whole
volume 200 mL.
BLANK PROCESS OF MERCURY COLLECTOR TUBE
Before the sampling, make sure to reduce the amount of blank of mercury collector tube.
(For the detail, refer to the instruction manual of MA-3 Solo)
OPERATIONAL PROCEDURE
During the installation, do not close the inside cylinder of the
1st impinger.
Do not insert/remove the mercury collector tube during the
measurement. It can affect the measurement result.
When handling a mercury collector tube for a measurement or
the like, take care not to get burned.
When handling, put on protectors such as heat-resistant gloves,
and use boat tongs.
The sampling method is the same for calibration curve measurement and unknown sample
measurement.
(1) Turn on MA-3 Solo and PC. Start “MA3SoloWin”.
(2) Perform the Self Check.
(3) Drip 20mL of 1mol/L KOH solution to the 2nd Impinger. Fix with the Joint Clamp.
Caution

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation 8
(4) Attach the mercury collector tube to the mercury collector tube connecting tube.
(5) Drip “20 mL of distilled water and standard mercury liquid” or “20mL of unknown
sample” into the impinger.
(6) Add 1 mL of 1:1 sulfuric acid and 1 mL of 10% stannous chloride solution.
(7) Select “COLLECTOR TUBE / AQUA kit” as the measurement method. Click “START”
button.
(8) Close the inside cylinder of the impinger.
*Check if it is bubbling.
If not, there is a leakage. Open the impinger inside cylinder, stop the sampling, and
fix the leakage. (Refer to 5.4 LEAK CHECK)
(9) When the measurement is completed, remove the inside cylinder of the impinger or
remove Mercury Collector Tube.
(10) Attach the mercury collector tube to the holder. Measure it with MA-3 Solo.
(For the detail, refer to the instruction manual of MA-3 Solo)
METHOD: COLLECTOR TUBE

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation 9
The calibration curve can be created by measuring the standard solution by the thermal
vaporization method or mercury collector tube measurement by using this unit.
However, the atomization method can be varied due to the deterioration or the filling
condition of the mercury collector tube. For the accurate measurement, create the
calibration curve by mercury collector tube measurement by using this unit.
(11) Discard the effluent after the measurement into a tank or the like, and clean the outside
cylinder and inside cylinder of the impinger.
*If the stannous chloride solution is left, mercury will volatilize during the next
measurement and a precise and accurate measurement will be impossible.
<Sampling of 2nd time and after>
Repeat step 4) - 11).
<To perform the sampling and measurement at the same time>
1) Install the mercury collector tube to measure to MA-3 Solo.
2) Perform the sampling.
3) After the measurement, remove the mercury collector tube from MA-3 Solo, and
install the mercury collector tube that has finished sampling.

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation 10
CLEANING OF GLASSWARE
On returning from on-site work, be sure to carry out the alkaline cleaning and acid cleaning
of the glassware. Insufficient cleaning may cause the dispersion of measurement values.
The mercury collector tube cannot be cleaned. Perform the blank process after the usage.
(Refer to “4.4 BLANK PROCESS OF MERCURY COLLECTOR TUBE”)
(1) After rinsing the glassware with water, put the glassware in a container into which
alkaline detergent has been poured, and carry out ultrasonic cleaning for 30 minutes.
If alkaline detergent is not available, 0.1 mol/L sodium hydroxide solution will also be
effective.
If an ultrasonic cleaner is not available, keep the glassware in detergent overnight, and
then scrub the glassware using a brush.
(Brushing after ultrasonic cleaning is more effective.)
(2) After rinsing the glassware sufficiently with water, carry out ultrasonic cleaning for 30
minutes using nitric acid (1:10) in the same way.
(3) After rinsing the glassware with water, rinse the glassware with pure water.
(4) Carry out drying and keep the glassware free of dust and pollution from air.

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation 11
5. MAINTENANCE
DAILY INSPECTION
Classification
Relevant Item
Inspection Standard
Measures Against
Abnormality Etc.
Inspection
Before Start
of Work
Tube
There shall be no dirt or wetting.
Cleaning
The tube shall not be disconnected.
Connection
Warning/error
A warning or an error shall not
occur at a startup.
See the instruction manual of
the MA-3 Solo.
Reagent
There shall be no cloudiness.
Disposal of reagent, cleaning
of container
Glassware
There shall be no crack or chip.
Disposal
Mist catcher
Waterdrops shall not stick.
Replacement
Checking at
Time of
Measurement
Bubbling
Bubbling shall be carried out.
Checking of tube connection.
Checking of impinger
connection.
Calibration curve
The correlation coefficient (r) shall
be 0.999 or more.
Preparing new reagents.
Replacement of mercury
collector tube.
RECOMMENDED REPLACEMENT COMPONENTS
We recommend that the following components be replaced periodically to use the product
stably, though their lifetimes vary depending on the usage condition:
*The recommended replacement period is not the guaranteed lifetime.
ERRORS AND WARNINGS
These messages are the same as that of the MA-3 Solo main unit. For details, see the
instruction manual of the MA-3 Solo main unit.
Name
Recommendation
Replacement Criterion
1
Mist catcher
1 year
When waterdrops have stuck
2
Impinger inside
cylinder
2 years
When the diffuser tube has been clogged.
3
Impinger outside
cylinder
2 years
When it is impossible to remove white dirt
4
Tube piping
0.5 year
When the sensitivity has lowered or high-
concentration contamination has occurred

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation 12
LEAK CHECK
This is the method to check the leakage of this unit. For the leak check of MA-3 Solo, refer
to the instruction of MA-3 Solo.
(1) Open Hardware Check window. (MA3 Solo Win - Maintenance – Hardware Check)
(2) Connect the tubing as follows.
PTFE tube and Tygon tube
Connect 2nd impinger OUT – MA-3 Solo INLET
Bent Tygon tube
PTFE tube and Tygon tube
Connect 2nd impinger OUT – MA-3 Solo INLET

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation 13
(3) In Hardware Check window, Check if the reading of FLOW METER is decreased.
If not, there is a leakage.
※It takes 3-4min for the reading to become 0L/min.
(4) Slowly open the inlet of 1st impinger.
6. SPECIFICATION
Measurement Range
5ng/L - 50 g/L (for measurement of 20 mL)
Precision
RSD 3% (for 15ng/L or more)
Sample Volume
20 mL
Dimensions
464366176 (carrying case)
Weight
6 kg(※includes the carrying case)

Instruction Manual： AQUA kit for MA-3 Solo NIC-TD-0000187-01
Nippon Instruments Corporation 14
7. CONTACT ADDRESS
Nippon Instruments Corporation
14-8 Akaoji-cho Takatsuki-shi, Osaka, 569-1146 Japan
Please contact the distributor or the following.
Technical Service
E-mail
shar[email protected].jp
URL
https://www.hg-nic.biz