Okolab H301-LG-DLS User manual

H301-LG-DLS User Manual
September 2016
www.oko-lab.com
Page1
H301-LG-DLS
Compatible with the following XY stages
Compatible with the following Okolab Controllers
LEICA super Z Galvo stage (SP5/SP8)
H301-T-BL-PLUS
UNO-STAGE-TOP-INCUBATOR
H401-T-DUAL
Vers. 01.16

H301-LG-DLS User Manual
September 2016
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Index
1. Components and dimensions................................................................................................... 3
2. Sample Holders................................................................................................................... 4
2.1 Available Sample Holders....................................................................................................... 4
3. Insertion of the Sample Feedback Temperature Sensor.................................................................. 5
4. Changing the Microscope Pillar Cover........................................................................................ 6
5. Removing and Inserting the Lid ............................................................................................... 6
6. Insertion of Sample Holder into Chamber ................................................................................... 7
7. Working with 35 or 60 mm Petri Dishes ....................................................................................10
8. Connection of the Gas Supply ................................................................................................12
9. Working with Perfusion ........................................................................................................12
10. Connection of the Chamber with Super Z-Galvo stage...................................................................12

H301-LG-DLS User Manual
September 2016
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1. Components and dimensions
H301-LG-DLS includes the following components:
Chamber base with embedded temperature sensor
Chamber body, it owns 12 holes for the insertion of perfusion tubing up to 2.0 mm
Objective adapter, it fits Leica HC APO L 10x/0.3 W or Leica HC FLUOTAR L 25x/0.95 W.
Sample Holder, it allows the Petri to be hold in the chamber.
Lid, it features a magnetic system allowing to lift and release the Lid surrounding the objective, together with
it.
Total chamber weight, 133.15g.
Figure 1. H301-LG-DLS Components and Dimensions.

H301-LG-DLS User Manual
September 2016
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2. Sample Holders
2.1 Available Sample Holders
The following sample holders are available.
NOTE: Please contact [email protected] if you cannot find the sample holder you are looking for. We are constantly
adding new inserts to the list.
1x35-DLS
#1 35mm Petri-dish
1x60-DLS
#1 60mm Petri-dish
Figure 2. Available sample holders.

H301-LG-DLS User Manual
September 2016
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3. Insertion of the Sample Feedback Temperature Sensor
Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H301-LG-DLS main body
(see Figure 3 Frontal and 3D views).
1. Frontal View
2. 3D View
Figure 3. Insertion of the temperature sensor inside the chamber.

H301-LG-DLS User Manual
September 2016
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4. Changing the Microscope Pillar Cover
The H301-LG-DLS is equipped with a magnetic Lid which directly holds into the provided Okolab Microscope Pillar Cov-
er. To install Okolab Microscope Pillar Cover follow the instructions as shown in Figure 4.
Figure 4. Changing the Microscope Pillar Cover
5. Removing and Inserting the Lid
The H301-LG-DLS is equipped with a magnetic Lid which directly holds into the provided microscope pillar cover.
If you want to access the specimen or you want to remove the sample holder, you should remove the Lid first; to re-
move it follow the steps shown in the images of and listed below:

H301-LG-DLS User Manual
September 2016
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Figure 5. Removing and Inserting the Lid.
6. Insertion of Sample Holder into Chamber
Sample holders fit into the chamber base and are held in place by captive screws embedded the holder. Screw the
sample holder using a 1.5 mm Allen key, don’t care about the orientation since the sample holder is symmetric, as
illustrated in Figure 6.

H301-LG-DLS User Manual
September 2016
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Figure 6. Introduction of the Specimen Holder inside the Chamber Base.
If you want to change the sample holder follow the steps shown in the images of Figure 7 and listed below:
1. Remove the chamber body by lifting it from the workspace, as shown in Figure 7 Image 1 and 2
2. Unscrew the sample holder captive screws using a 1.5 mm Allen key
3. Lift and replace the sample holder, as shown in Figure 7 Image 4

H301-LG-DLS User Manual
September 2016
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Figure 7. Introduction of the Specimen Holder inside the Chamber Base.

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September 2016
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7. Working with 35 or 60 mm Petri Dishes
Flat springs prevent movement of 35 and 60 mm dishes inside of the sample holder (See Figure 8 and Figure 9). To
place the Petri, please follow the steps below:
1. Slightly loose the sample holder screws denoted as A in Image 1 of Figure 8 and Figure 9, using a flat head screw-
driver, in order to have the locks released
2. Place the Petri in its holder as in Image 2 1 of Figure 8 and Figure 9
3. Block the sample holder screws denoted as B in Image 1 of Figure 8 and Figure 9, using a flat head screwdriver, in
order to have Petri locked in position
Figure 8. Plate adapter for 35 mm Petri-dish holder.

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Figure 9. Plate adapter for 60 mm Petri-dish holder.
Note. On the sample holders for petri dishes there are two water reservoirs (see Image 4 of Figure 8 and Figure 9). Fill
them with water to ensure a high humidity percentage inside the chamber.

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8. Connection of the Gas Supply
A single silicon tubing carries output gas from the Okolab Gas Controller to the H301-LG-DLS. Silicon tubing connects
to a gas input - brass opening - located on a corner of the H301-LG-DLS. See Figure 10. Connect by gently pushing sili-
con tubing onto brass opening.
Figure 10.Connection with gas supply.
9. Working with Perfusion
The H301-LG-DLS features 12 perfusion holes on the sides for the insertion of perfusion tubing up to 2.0 mm in outer
diameter. Small screws plug the perfusion holes when not in use. (Grub screws M2,5x3). Remove small screws as nec-
essary before introducing perfusion tubing.
Figure 11 shows the location of perfusion holes.
1. Frontal View
2. 3D View
Figure 11. Perfusion
10. Connection of the Chamber with Super Z-Galvo stage
H301-LG-DLS fits on the Super Z-Galvo stage. It can be screwed onto the Galvo Stage as shown in Figure 12; place the
chamber on the stage and screw the four captive screws. Screws housings are indicated with letter A in Figure 12.

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September 2016
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Figure 12. How to fix the H301-LG-DLS on the Super Z Galvo stage