Okolab H301-PRIOR-NZ100-H117 User manual

H301-PRIOR-NZ100-H117 User Manual
REV 02
www.oko-lab.com
Page1
H301-PRIOR-NZ100-H117
Compatible with the following Z piezo stages
Compatible with the following Okolab Controllers
•Prior: Prior NZ100; NZ 200 in H117
•H301-T-BL-PLUS
•UNO-COMBINED-CONTROLLER
•H401-T-DUAL

H301-PRIOR-NZ100-H117 User Manual
REV 02
www.oko-lab.com
Page2
Index
1. Components and dimensions................................................................................................... 3
2. Insertion of the Sample Feedback Temperature Sensor.................................................................. 4
3. Working with 35 Petri Dish-.................................................................................................... 4
4. Working with 1”x3’’ and 1”x2’’chamber slides............................................................................ 5
5. Connection of the Gas Supply ................................................................................................. 6
6. Working with Perfusion ......................................................................................................... 6
7. Connection of the Chamber with Z piezo stage ............................................................................ 7

H301-PRIOR-NZ100-H117 User Manual
REV 02
www.oko-lab.com
Page3
1. Components and dimensions
H301-PRIOR-NZ100-H117 includes the following components:
•Chamber main body with embedded temperature sensor
•Heated glass Lid with Indium Tin Oxide (ITO) conductive coating and embedded temperature sensor
•Chamber riser. It is a removable frame increasing the height of the chamber from 23 to 28 mm
Distance between focal plane
and upper side of the glass
lid without the chamber riser
Distance between focal plane
and upper side of the glass
lid with the chamber riser
Chamber top view
Lid
Chamber Riser
Chamber Main Body
Sample Holder-Order Separately
Figure 1. H301-PRIOR-NZ100-H107 Components and Dimensions.

H301-PRIOR-NZ100-H117 User Manual
REV 02
www.oko-lab.com
Page4
2. Insertion of the Sample Feedback Temperature Sensor
Insert the Sample Feedback Temperature Sensor through one of 12 perfusion holes (see par 6. Figure 7) located in the
H301-PRIOR-NZ100-H107 chamber riser (see Figure 2, Frontal and 3D views). Small screws plug the perfusion holes
when not in use. (Grub screws M3x6). Remove small screw as necessary before introducing the sample Feedback Tem-
perature Sensor.
1.Frontal view 2.3D view
Temperature Sensor dedicated opening
Temperature Sensor dedicated opening
Figure 2. Insertion of the temperature sensor inside the chamber.
3. Working with 35 Petri Dish
The closing parts (A in Image1 of Figure 3) and the flat springs (B in Image 3 of Figure 3) prevent movement of 35 dish
inside of the sample holder (See Figure 3)
1 2 3
A
B
Figure 3. Plate adapter for 35 mm Petri-dish holder.

H301-PRIOR-NZ100-H117 User Manual
REV 02
www.oko-lab.com
Page5
4. Working with 1”x3’’ and 1”x2’’chamber slides
Flat springs prevent movement of 1”x3’’ and 1”x2’’ chamber slides inside of the sample holder.
A
A
12
Figure 4. Sample holder for1”x3” chambered cover glass holder.
21
AA
Figure 5. 1 Sample holder for 1”x2” chambered cover glass holder.

H301-PRIOR-NZ100-H117 User Manual
REV 02
www.oko-lab.com
Page6
5. Connection of the Gas Supply
A single silicon tubing carries output gas from the Okolab Gas Controller to the H301-PRIOR-NZ100-H117. Silicon tubing
connects to a gas input - brass opening - located on a corner of the H301-PRIOR-NZ100-H117. See Figure 6. Connect by
gently pushing silicon tubing onto brass opening.
Gas Input
Figure 6.Connection with gas supply.
6. Working with Perfusion
The Chamber Riser included with H301-PRIOR-NZ100-H117 features 12 perfusion holes for the insertion of perfusion
tubing up to 2.5 mm in outer diameter. Small screws plug the perfusion holes when not in use. (Grub screws M3x6).
Remove small screws as necessary before introducing perfusion tubing.
Figure 7 shows location of perfusion holes.
1.Frontal view 2.3D view
Perfusion dedicated opening
Figure 7. Perfusion

H301-PRIOR-NZ100-H117 User Manual
REV 02
www.oko-lab.com
Page7
7. Connection of the Chamber with Z Piezo Stage
Follow the steps shown in the images of Figure 8 and listed below in order to correctly connect the chamber with Z
piezo stage:
1. Place the sample holder on the stage and tighten the four M2x3 Pan head screws, as shown in Figure 8 Image 1
2. Place the chamber main body on the stage and tighten the two M2x3 Pan head screws, as shown in Image 2 and 3
Figure 8
3. Place the lid on the chamber, as shown in Image 3 of Figure 8
1 2
4
3
Screw M2x3
Screw M2x3
Figure 8. How to assemble the chamber to the stage.

H301-PRIOR-NZ100-H117 User Manual
REV 02
www.oko-lab.com
Page8
8. Cleaning
•Turn the system off and pull the mains plug out the socket
•Remove the lid from the chamber main body, and keep it separate from the chamber main body while the
chamber cools down.
•To clean the body and the glass lid of the chamber, wipe with a soft micro-fiber cloth. For stubborn smudges,
you can damp the soft micro-fiber cloth with ethyl alcohol (product code UN1170). Do not put any liquid di-
rectly on the chamber. While cleaning the glass lid, do not apply strong force to the surface of the glass lid
because it can be damaged.
H301-PRIOR-NZ100-H117_User Manual Rev 02_Published in January 2020